Cp4.1LG20g00650 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG20g00650
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	t-SNARE coiled-coil homology domain-containing protein
Location	Cp4.1LG20: 298428 .. 303603 (-)
RNA-Seq Expression	Cp4.1LG20g00650
Synteny	Cp4.1LG20g00650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAAGCAGAAAGATGACAAAGATATAGTTCATAGATAGTGAAAGTTGACTTTCTAAGGCGATCAAAGTTGAATTTCAGTCTGAGATTATTCATCGCTCCCTAATTTTTGACCAAATCCATCAACACTGTTTGATCATTTTTTTTTATCCAAAAGATGAATAAGCGGTTAATGACATAAGGTTTGTTTACAGCGCGTCTTCATCGTTGAACTTCCTCGTGGGTGCTAGTTTCCTCTTTCAACAATTTTCTCAAACGCGGTTTCTTGATTGATTCTCTCGTTGCCCGAGTCGTCTATTTTGAACCAACCCACTTCTCTCCATCGCGCCCTTAAATTCTACGATTCGGTTTTGATTTTGCAGTTTCTTAGATTTTGAGTTTGTGGAATTGTTCTGTTCCCTTCGTTCTCTGAACTCTCAAGGTACTCCAATTCTTTTCTTTCTTGGATTTTTGTATCTGGGTATTCATCATCAACTTCCTTCTTCGCCTTTACATGATTTTGTTTGTTAGCTTCTTGTCCAGTTTAGTTTTTGATCGTGCTATCATCTCTTCTGTTCTCACATCCATTCGTCCATTTTGGATCAATTTCTGCTGGAAAACTGATTGAAGTAGTTTAAATAAGAATCTAAATCTTAGAACATCCCACTGTTCTTCAATGAAATTGACTGTTTTGATCGATTTTCCCTTATTGTATTTTGTTTCTTCTGCTGACATGATCTTGATATCTTCCATTTGCAGGAAAAAAACTGTCACCAATTTTTTGCATTCTAAACCCTTGGGAAAATGTTTGGTTCAAGGAAGGCTCATTTGAGGATAGGAAAACATAGTTCTACCCCGAACCCTTTTGAGTCTGACGATGGTGTTAAAGCTAAGCAAAACCAGGTTTCTGCACAAAGGACTTCGTCAGACCCTTCATTAGTGACGGAGAATTTTGGCTCCAATCCTTTTGATGATGATCATGGACATGCTCCCTCTTCTTCAGGTTCACTAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTCCGTGACTCGGGAGGAATAGAAAACCAATCAGTGCAGGAGCTGGAGAATTATGCTGTTTATAAGTCTGAGGAGACAACAAAAGCAGTGAATGGCTGTCTAAAGATTGCTGAAGAGATAAGGGATGATGCTACCCAAACTTTGGTTGCCTTGCATCAGCAGGGTGAGCAAATTAACAGGACTCATATGGTTGCTGCTGACATTGATTATGACCTCAGTCGGGTATGTTCGAAATCCCATTTGTTCACTTTTATGTCTCGGGAGGACTCGACGAAATGCCAAGGATTTGATCTTGGTGTTTCTTTGGTCTAGAAATTACTAGCATTAACTTTGCCTGTATATGTCTAAGCTCTATCATATCTTCATTTGCGTGATGTATTAATCTCCTGTAACTCTATTTTCTAAGTGAGTCTTTTCATGAATGCAATATCAGGGAGAGAAACTGCTGGGAAATCTAGGGGGAATGTTCTCTAAGACATGGAAGCCAAAGAAAACTCGACCAATATCAGGACCTGTTATAAGCTCAGGTTAGCCACTTTCTGCTCATGAGATTCAGGGTTAGATGGTAACTTGTTGAAATTCTGTGCAACTGATCTGTTTATTCCCATTTTATAGAGGATTTAGTTAGAAGAAAGGGCAACCACATGGAGCAGAAGGAAAAGTTAGGATTGACACGTCATAAGGACCGGACCAATACAAAAGCTGCATATGCCGAACCTACCAGTGCAATTCAAAAAGATGAGGTTTGTATTTGCTATCTGTAAATAGTTACTTCAAAACGGTACATTGGAGCATATCAACCTAAACATATTTCAACTATTTAAAATATATGTCTTCGACTAAGAGGTCAAAGGTTCGAAATCTACCACCCTCTCAGCTTATGGGTGGCAGGAATTATTGCATTCCGCATAGATTTGTGGTTTTAGTCATTCTTGAAGATACTGTTGCACAAATCTGTACCATGATACCCAAAGCATAATGATCATTTCCAAAATGCAGTATCATTCGTCTTACATATGCTTTGTGTTTGAAAATTACATACACAGGTAGAAAAGGTGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTAGAGCTGAAGGAAATGGCTGTTGACATGGGTTGTGAAATTGATAGGTTAGTTCGTTTCTATATTCCATAATAACTCGTGTGCTTGTTCTTGCAAATCAAAGACATAAAGACAAGTGTGTACATTAGATATTGAGATGATTTGTTGCTTATTACAGGCAGAACCATGCTCTGGATCACGCTTCTGTTGATGTTGAGGTGATCGGTGACCGAGTTAAAGATGCCAACCGACGGGGTCGTCGTCTGCTCGGGAAATAGAGTTCAAGGCTCTCACATTTGTGATTAGCTTGCTGATACTTATGTACATCTTCCATTTTCCTGGCTTTGTTTTCTTTTTACTTTCAGAAATAGGTATTACCCCATGTCCTAGCTGACGTTGTGTGTTTGAGATCACTAGTTACTTGACTAGGACTAGAAGGTAACTGCACTGATTTTATCATTCTTAGTTCATCTTGAGCAGTGTATACAAACAACCACATTCGAACAATTTCATTTCAATAAATTAGAAACGTGCATTTTCGAACACGGGATGGTTTACCGATCCGAGTTCTGATGGGTTCAAATTCCTAGAACCCCAGACAAACCTACCAGAAAAAATTAGAACTCAGAGGGCCTGGGCGCTTCGGAACCTCGAGCTGAGCTCTGCCGTCGGCGTAGGCGGAGGATATCGTTGAAGGGTCGGAGAGGCTCCGACTTTCTCGGAAGGTATCAATGGTGCAGGGTATGGTGTACTCTCTGATGTTGTAATCGGGAAATTGACGCGCTATTCGCAGGAGAGAACGAAAGAGAGAGAGGATTCGGATCCCGACGGGGATGCCATCTCCGGCGAGAGACGAAAAGTATACTAAGAGATTTCCATTTTCCCATCATATCTTCTCTTACATTCAACTACTCCATAACTAATCTAAAGATCATCCCTGCCCCAAATAAATATTTATCATAAACTTATGATCATCCATGTTCTTGAACTTGTTCTTTTAACTCTAAAAATCATAGCTTTTGCAGATTTATTAATGATCAATAGGGAGAAATCCTTGACGAAAAGAAGGGTTCCATTTTTTAAAATTTGGGAAGTGGGTTAAGCTAAACAAAGAGAAAAAGAAGAAAAAGGTTTTGAGTGTACATAGAAATAGAATCAATGGAAGGCGATGAAGAAGAAAATGCCTTGCTTCGGAATGGAGTTGAGTGCAAAGAAGGCATTTCCTTCGCTTCGCAACTTCCCTGCGTAAAGATACAATACATATTAACTTCATAAACAGTTTCAGAAATTTGAGAGGTATCGATATAAGCTTGAGATTTCAGCACATGGAAACTTTGACTCATCAAAGAAGGTATACTTTTCTTATCTCTTGATCTCCATTCCCCAGTTTGAATATTTGATGTATGTTCATCACTCTTTCATCTGGTTCATCATCATTTACAACCCAAAAAGTTTTACAAGGGATAAAATGATAAGAATCATTGAAACAGAAAAGTAATCGAATCTGATCTCTGAGTCACCATTGGCCAAACTCAGCTCAGATTAGGTTCTACAACACCAAAGAGTTTCCTCTTCTTGTCCTCTTTTATTTGAATACTGAAAGTAATATGGCTATTGACTGCTTTGTAGGCTGAGTTCTTCGGATTCAGGACGACATAATAAAGATGCTTCCATCAAACCAAGAGATGGAATTCCACAGCAAAAAACAAGATCCTCCAAAGAGGGTACTGATAAAAAGGAGTCCAAGAAGTTACGGTGGTATTTTGCAAACGAAATGAATGAGGATTATGATTCAAGGGATTTAGAGTTTGCCACTGCAGCAGCATCAGCTGCATTTGCAATTCGCTCACAAGAAGAAACTGATTTACAATACCAGAAAAAGAAGAGAGGAAGTCTGGAAACGCCATTAACAAAGGTTAAAACCAGAAAAGATGACATAACCAGGCGACCGTCTAACAAAGAAACAACAAATCCTGGTAAAATTTAAACATTGTGCTTAGAAATTTCTTATAAGAATTTCTTGTCACAAAAAGTATCATGTTCATTACACCAGGCCAATCTTCAATAAAGAAGCCAACGGGACATGAAAAGGACTCAATAACTGGAATTCCTTTACCGCCACCAAGACGAAGCCTTGTAGAAACTAGAGCTGATGTGTGGGAGAGGCATAACATGGAGAAGATTAGAAAAAGGTATATACTAATTTCTGCATTAATCAATACTCAGGAGTGGACCTCACAGTTCCAAGTTCTCAAGTTCATGAAACACTTGCACTAAAAGATCTCTCCTCTTAATCCTGAAACTTGCTTATGGAGTTGTTTTCTAAGACAATCTCATATTTCAGGTACGAAAAGATGAAATTGTCAATTCATGCCTGGGAGAATAAGAAGAAAATGCATGCCAAACTTCATAAGGAAAAGAAAATGGTCTTACCCCACCTTTTGGATGTTTTCCTTACTATAATACATTTTAATGTCAACTGAAAAAAACTTCTGTTCATGAAGGCTGAATTGGAGAACAAAAAGGCACTGTTTCTGCAAAATTACCAGGACAACATAGTACGAATCGACCAGATCACGGAGGGAGCAAGGGCACAACTAGAACAAAAAAGAAGAAGGGAAGAGAAGAAAGCAAGAGAAACAGCTAACCGAATCAGGTCGACCGGAAGACTTCCTGTGACTTGTTTCTGCATCCAGTATCACTAACAATGAGACAGTCCTGGTGAAAAGAGAAGCATCAAATCATGCAGCTCCTATTCACTTACACTTCACCTAACAAATTCCAATCCAAAATCAATAACAAGTCATTGTGATGTTGTGTAAATTGTTTCTTATGTTTCTTTCATTGTGGCCTCAAACTCACTCTCAAAGAATGATAGATTTGATTGCAATAACAGAGATAAGTACAGCTTTAATCTTCTAGAATTTTTGCATTGATGTGAGTTTGGTATGGCACAAAATCATGGAGCAAATTAATCAATCATTTCACTTTTTAAACAATAATAATAATGAATCCACCACACAATAAATATAATTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

mRNA sequence

CAGAAGCAGAAAGATGACAAAGATATAGTTCATAGATAGTGAAAGTTGACTTTCTAAGGCGATCAAAGTTGAATTTCAGTCTGAGATTATTCATCGCTCCCTAATTTTTGACCAAATCCATCAACACTGTTTGATCATTTTTTTTTATCCAAAAGATGAATAAGCGGTTAATGACATAAGGTTTGTTTACAGCGCGTCTTCATCGTTGAACTTCCTCGTGGGTGCTAGTTTCCTCTTTCAACAATTTTCTCAAACGCGGTTTCTTGATTGATTCTCTCGTTGCCCGAGTCGTCTATTTTGAACCAACCCACTTCTCTCCATCGCGCCCTTAAATTCTACGATTCGGTTTTGATTTTGCAGTTTCTTAGATTTTGAGTTTGTGGAATTGTTCTGTTCCCTTCGTTCTCTGAACTCTCAAGGAAAAAAACTGTCACCAATTTTTTGCATTCTAAACCCTTGGGAAAATGTTTGGTTCAAGGAAGGCTCATTTGAGGATAGGAAAACATAGTTCTACCCCGAACCCTTTTGAGTCTGACGATGGTGTTAAAGCTAAGCAAAACCAGGTTTCTGCACAAAGGACTTCGTCAGACCCTTCATTAGTGACGGAGAATTTTGGCTCCAATCCTTTTGATGATGATCATGGACATGCTCCCTCTTCTTCAGGTTCACTAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTCCGTGACTCGGGAGGAATAGAAAACCAATCAGTGCAGGAGCTGGAGAATTATGCTGTTTATAAGTCTGAGGAGACAACAAAAGCAGTGAATGGCTGTCTAAAGATTGCTGAAGAGATAAGGGATGATGCTACCCAAACTTTGGTTGCCTTGCATCAGCAGGGTGAGCAAATTAACAGGACTCATATGGTTGCTGCTGACATTGATTATGACCTCAGTCGGGGAGAGAAACTGCTGGGAAATCTAGGGGGAATGTTCTCTAAGACATGGAAGCCAAAGAAAACTCGACCAATATCAGGACCTGTTATAAGCTCAGAGGATTTAGTTAGAAGAAAGGGCAACCACATGGAGCAGAAGGAAAAGTTAGGATTGACACGTCATAAGGACCGGACCAATACAAAAGCTGCATATGCCGAACCTACCAGTGCAATTCAAAAAGATGAGGTAGAAAAGGTGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTAGAGCTGAAGGAAATGGCTGTTGACATGGGTTGTGAAATTGATAGGCAGAACCATGCTCTGGATCACGCTTCTGTTGATGTTGAGGTGATCGGTGACCGAGTTAAAGATGCCAACCGACGGGGTCGTCGTCTGCTCGGGAAATAGAGTTCAAGGCTCTCACATTTGTGATTAGCTTGCTGATACTTATGTACATCTTCCATTTTCCTGGCTTTGTTTTCTTTTTACTTTCAGAAATAGGTATTACCCCATGTCCTAGCTGACGTTGTGTGTTTGAGATCACTAGTTACTTGACTAGGACTAGAAGGTAACTGCACTGATTTTATCATTCTTAGTTCATCTTGAGCAGTGTATACAAACAACCACATTCGAACAATTTCATTTCAATAAATTAGAAACGTGCATTTTCGAACACGGGATGGTTTACCGATCCGAGTTCTGATGGGTTCAAATTCCTAGAACCCCAGACAAACCTACCAGAAAAAATTAGAACTCAGAGGGCCTGGGCGCTTCGGAACCTCGAGCTGAGCTCTGCCGTCGGCGTAGGCGGAGGATATCGTTGAAGGGTCGGAGAGGCTCCGACTTTCTCGGAAGGTATCAATGGTGCAGGGTATGGTGTACTCTCTGATGTTGTAATCGGGAAATTGACGCGCTATTCGCAGGAGAGAACGAAAGAGAGAGAGGATTCGGATCCCGACGGGGATGCCATCTCCGGCGAGAGACGAAAAGTATACTAAGAGATTTCCATTTTCCCATCATATCTTCTCTTACATTCAACTACTCCATAACTAATCTAAAGATCATCCCTGCCCCAAATAAATATTTATCATAAACTTATGATCATCCATGTTCTTGAACTTGTTCTTTTAACTCTAAAAATCATAGCTTTTGCAGATTTATTAATGATCAATAGGGAGAAATCCTTGACGAAAAGAAGGGTTCCATTTTTTAAAATTTGGGAAGTGGGTTAAGCTAAACAAAGAGAAAAAGAAGAAAAAGGTTTTGAGTGTACATAGAAATAGAATCAATGGAAGGCGATGAAGAAGAAAATGCCTTGCTTCGGAATGGAGTTGAGTGCAAAGAAGGCATTTCCTTCGCTTCGCAACTTCCCTGCGTAAAGATACAATACATATTAACTTCATAAACAGTTTCAGAAATTTGAGAGGTATCGATATAAGCTTGAGATTTCAGCACATGGAAACTTTGACTCATCAAAGAAGGCTGAGTTCTTCGGATTCAGGACGACATAATAAAGATGCTTCCATCAAACCAAGAGATGGAATTCCACAGCAAAAAACAAGATCCTCCAAAGAGGGTACTGATAAAAAGGAGTCCAAGAAGTTACGGTGGTATTTTGCAAACGAAATGAATGAGGATTATGATTCAAGGGATTTAGAGTTTGCCACTGCAGCAGCATCAGCTGCATTTGCAATTCGCTCACAAGAAGAAACTGATTTACAATACCAGAAAAAGAAGAGAGGAAGTCTGGAAACGCCATTAACAAAGGTTAAAACCAGAAAAGATGACATAACCAGGCGACCGTCTAACAAAGAAACAACAAATCCTGGCCAATCTTCAATAAAGAAGCCAACGGGACATGAAAAGGACTCAATAACTGGAATTCCTTTACCGCCACCAAGACGAAGCCTTGTAGAAACTAGAGCTGATGTGTGGGAGAGGCATAACATGGAGAAGATTAGAAAAAGGTACGAAAAGATGAAATTGTCAATTCATGCCTGGGAGAATAAGAAGAAAATGCATGCCAAACTTCATAAGGAAAAGAAAATGGCTGAATTGGAGAACAAAAAGGCACTGTTTCTGCAAAATTACCAGGACAACATAGTACGAATCGACCAGATCACGGAGGGAGCAAGGGCACAACTAGAACAAAAAAGAAGAAGGGAAGAGAAGAAAGCAAGAGAAACAGCTAACCGAATCAGGTCGACCGGAAGACTTCCTGTGACTTGTTTCTGCATCCAGTATCACTAACAATGAGACAGTCCTGGTGAAAAGAGAAGCATCAAATCATGCAGCTCCTATTCACTTACACTTCACCTAACAAATTCCAATCCAAAATCAATAACAAGTCATTGTGATGTTGTGTAAATTGTTTCTTATGTTTCTTTCATTGTGGCCTCAAACTCACTCTCAAAGAATGATAGATTTGATTGCAATAACAGAGATAAGTACAGCTTTAATCTTCTAGAATTTTTGCATTGATGTGAGTTTGGTATGGCACAAAATCATGGAGCAAATTAATCAATCATTTCACTTTTTAAACAATAATAATAATGAATCCACCACACAATAAATATAATTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Coding sequence (CDS)

ATGTTTGGTTCAAGGAAGGCTCATTTGAGGATAGGAAAACATAGTTCTACCCCGAACCCTTTTGAGTCTGACGATGGTGTTAAAGCTAAGCAAAACCAGGTTTCTGCACAAAGGACTTCGTCAGACCCTTCATTAGTGACGGAGAATTTTGGCTCCAATCCTTTTGATGATGATCATGGACATGCTCCCTCTTCTTCAGGTTCACTAACTTGGTCAGAAAGGAACAAGTACAAGAATGATTTCCGTGACTCGGGAGGAATAGAAAACCAATCAGTGCAGGAGCTGGAGAATTATGCTGTTTATAAGTCTGAGGAGACAACAAAAGCAGTGAATGGCTGTCTAAAGATTGCTGAAGAGATAAGGGATGATGCTACCCAAACTTTGGTTGCCTTGCATCAGCAGGGTGAGCAAATTAACAGGACTCATATGGTTGCTGCTGACATTGATTATGACCTCAGTCGGGGAGAGAAACTGCTGGGAAATCTAGGGGGAATGTTCTCTAAGACATGGAAGCCAAAGAAAACTCGACCAATATCAGGACCTGTTATAAGCTCAGAGGATTTAGTTAGAAGAAAGGGCAACCACATGGAGCAGAAGGAAAAGTTAGGATTGACACGTCATAAGGACCGGACCAATACAAAAGCTGCATATGCCGAACCTACCAGTGCAATTCAAAAAGATGAGGTAGAAAAGGTGAAGCAAGATGATGCACTATCTGACATGAGCGATATCTTGGTAGAGCTGAAGGAAATGGCTGTTGACATGGGTTGTGAAATTGATAGGCAGAACCATGCTCTGGATCACGCTTCTGTTGATGTTGAGGTGATCGGTGACCGAGTTAAAGATGCCAACCGACGGGGTCGTCGTCTGCTCGGGAAATAG

Protein sequence

MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHGHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK

Homology

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BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot
Match: Q9S7P9 (SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.0e-85
Identity = 175/303 (57.76%), Postives = 220/303 (72.61%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKH------SSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNP 60
           MFG RK+   + KH      SS PNPF+SDD    K     ++RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADM---TNP 60

Query: 61  FDDDH---GHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVN 120
           F  +    G + SS  SL  + + +YKN+FRDSGGIENQSVQELE YAVYK+EETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQI RTH  A +ID+DLSRGEKLLG+LGGMFSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPVISSEDLVRRKGNHMEQKEKLGL-TRHKDRTNTKAAYAEPTSAIQKDEVE 240
           PKKTRPI+GPV++ +D   R+ NH+E++EKLGL +  + ++ T+    E   A Q+ E+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRL 294
           K KQDD LSD+SDIL ELK MAVDMG EI++QN  LDH   DV+ +  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

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BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot
Match: Q9LMG8 (Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNAP30 PE=3 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.4e-71
Identity = 147/259 (56.76%), Postives = 186/259 (71.81%), Query Frame = 0


Query: 35  SAQRTSSDPSLVTENFGSNPFDDDHGHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQE 94
           + +RTSS+P L+T +     FDDD                +KYKN F DSGG+++Q+ +E
Sbjct: 27  AGRRTSSEPILITPD-----FDDD----------------DKYKNGFNDSGGLQSQTTEE 86

Query: 95  LENYAVYKSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSR 154
           LE YAVYK+EETTK VN CLKIAE+IR D  +TL  LHQQGEQINRTH +A D+D DLSR
Sbjct: 87  LEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSR 146

Query: 155 GEKLLGNLGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTK 214
           GEKLL NLGGMFSK WKPKKT+ I+GP+I+ +   ++  NH E++EKLGL   K R++++
Sbjct: 147 GEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLGA-KGRSSSQ 206

Query: 215 AAYAEPTSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVE 274
            A  +PT+A+QK E EK KQDD LSD+SDIL +LK MAVDMG EID+QN ALDH   DV+
Sbjct: 207 PALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVD 263

Query: 275 VIGDRVKDANRRGRRLLGK 294
            +  RV+ AN+R R LL K
Sbjct: 267 ELNSRVQGANQRARHLLSK 263

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BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot
Match: Q9SD96 (SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 1.8e-55
Identity = 125/253 (49.41%), Postives = 166/253 (65.61%), Query Frame = 0


Query: 42  DPSLVTENFGSNPFDDDHGHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVY 101
           D     ++F  NPFDDD                 ++ +  + SGG ENQ+VQELE+YAVY
Sbjct: 13  DEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQELESYAVY 72

Query: 102 KSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGN 161
            SEETTK V GCLK+AEEIR DA++TLV L++QG+QI RTH    D+D+ LSRGEK+LG 
Sbjct: 73  NSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGR 132

Query: 162 LGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRH-KDRTNTKAAYAEP 221
           LGG+FS+TWKPKK+R I+GPVI+  D  +RK   ++ +EKLGL    K ++ T     E 
Sbjct: 133 LGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKT---LPEA 192

Query: 222 TSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRV 281
             A QK ++   KQD+AL+D+S +L ELK MAVDMG  I+RQ + LDH   + + +  RV
Sbjct: 193 VDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRV 251

Query: 282 KDANRRGRRLLGK 294
           K +N+R R LL K
Sbjct: 253 KQSNQRARYLLRK 251

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BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot
Match: Q17QQ3 (Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.0e-10
Identity = 55/201 (27.36%), Postives = 92/201 (45.77%), Query Frame = 0


Query: 92  VQELENYAVYKSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYD 151
           ++E++  A   ++E+ ++    L++ EE +D   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRHKDRT 211
           +   EK L +LG        P         + SS+   +  GN+ +        R  D  
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 NTKAAYAEPTSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASV 271
              A        +  D  E  + D+ L  +S I+  L+ MA+DMG EID QN  +D    
Sbjct: 131 EQMAISGGFIRRVTNDAREN-EMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIME 190

Query: 272 DVEVIGDRVKDANRRGRRLLG 293
             +    R+ +AN+R  ++LG
Sbjct: 191 KADSNKTRIDEANQRATKMLG 204

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BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot
Match: P60878 (Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 4.0e-10
Identity = 55/201 (27.36%), Postives = 92/201 (45.77%), Query Frame = 0


Query: 92  VQELENYAVYKSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYD 151
           ++E++  A   ++E+ ++    L++ EE +D   +TLV L +QGEQ+ R       I+ D
Sbjct: 11  LEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKD 70

Query: 152 LSRGEKLLGNLGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRHKDRT 211
           +   EK L +LG        P         + SS+   +  GN+ +        R  D  
Sbjct: 71  MKEAEKNLTDLGKFCGLCVCP------CNKLKSSDAYKKAWGNNQDGVVASQPARVVDER 130

Query: 212 NTKAAYAEPTSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASV 271
              A        +  D  E  + D+ L  +S I+  L+ MA+DMG EID QN  +D    
Sbjct: 131 EQMAISGGFIRRVTNDAREN-EMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIME 190

Query: 272 DVEVIGDRVKDANRRGRRLLG 293
             +    R+ +AN+R  ++LG
Sbjct: 191 KADSNKTRIDEANQRATKMLG 204

[Close]

BLAST of Cp4.1LG20g00650 vs. NCBI nr
Match: XP_023520322.1 (SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 577 bits (1487), Expect = 1.76e-207
Identity = 293/293 (100.00%), Postives = 293/293 (100.00%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. NCBI nr
Match: XP_022924192.1 (SNAP25 homologous protein SNAP33-like [Cucurbita moschata])

HSP 1 Score: 568 bits (1463), Expect = 8.04e-204
Identity = 290/293 (98.98%), Postives = 290/293 (98.98%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDD HG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDHHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
            APSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  QAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. NCBI nr
Match: XP_023000657.1 (SNAP25 homologous protein SNAP33-like [Cucurbita maxima])

HSP 1 Score: 553 bits (1424), Expect = 7.08e-198
Identity = 282/293 (96.25%), Postives = 283/293 (96.59%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRK HLRIGKH STPNPFESDDGVKAKQNQVSAQRTSSDPSLV ENFGSNPFDDDHG
Sbjct: 1   MFGSRKGHLRIGKHGSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVAENFGSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS SLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQI RTH+VAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQITRTHVVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLT HK RTNTKAAYAEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTHHKGRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRG RLL K
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGHRLLRK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. NCBI nr
Match: XP_038894894.1 (SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25 homologous protein SNAP33-like [Benincasa hispida])

HSP 1 Score: 530 bits (1366), Expect = 4.89e-189
Identity = 266/293 (90.78%), Postives = 280/293 (95.56%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRKAH RIGKHSSTPNPFESDDGVKA+QNQVS+QRTSSDPSL+TENF SNPFDDDHG
Sbjct: 1   MFGSRKAHFRIGKHSSTPNPFESDDGVKAQQNQVSSQRTSSDPSLLTENFSSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS SLTWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLV LH QGEQI RTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVTLHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           P+I+S+DLVRRKGNH+EQKE+LGLT HK RTNTK A+AEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PIINSDDLVRRKGNHLEQKERLGLTHHKGRTNTKTAFAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQN+ LD AS DVEV+GDRVKDANRRGRRLLG+
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNNVLDRASDDVEVLGDRVKDANRRGRRLLGR 293

[Close]

BLAST of Cp4.1LG20g00650 vs. NCBI nr
Match: KAG6584421.1 (SNAP25-likeous protein SNAP33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 531 bits (1368), Expect = 3.42e-184
Identity = 271/274 (98.91%), Postives = 271/274 (98.91%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDD HG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDHHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
            APSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  QAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVE 274
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVE
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVE 274

[Close]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL
Match: A0A6J1E891 (SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431714 PE=4 SV=1)

HSP 1 Score: 568 bits (1463), Expect = 3.89e-204
Identity = 290/293 (98.98%), Postives = 290/293 (98.98%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDD HG
Sbjct: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDHHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
            APSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  QAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL
Match: A0A6J1KE92 (SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC111495025 PE=4 SV=1)

HSP 1 Score: 553 bits (1424), Expect = 3.43e-198
Identity = 282/293 (96.25%), Postives = 283/293 (96.59%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRK HLRIGKH STPNPFESDDGVKAKQNQVSAQRTSSDPSLV ENFGSNPFDDDHG
Sbjct: 1   MFGSRKGHLRIGKHGSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVAENFGSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS SLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           RDDATQTLVALHQQGEQI RTH+VAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 RDDATQTLVALHQQGEQITRTHVVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           PVISSEDLVRRKGNHMEQKEKLGLT HK RTNTKAAYAEPTSAIQK EVEKVKQDDALSD
Sbjct: 181 PVISSEDLVRRKGNHMEQKEKLGLTHHKGRTNTKAAYAEPTSAIQKVEVEKVKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRG RLL K
Sbjct: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGHRLLRK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL
Match: A0A0A0LQ81 (t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039020 PE=4 SV=1)

HSP 1 Score: 518 bits (1333), Expect = 2.53e-184
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGSRK H R+GKHSSTPNP ESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDDDHG
Sbjct: 1   MFGSRKVHFRLGKHSSTPNPIESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS S TWSERNKYKNDFRDSGG+ENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSTTWSERNKYKNDFRDSGGVENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGG+FSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGIFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           P+ISS+DLVRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQK EVEK+KQDDALSD
Sbjct: 181 PIISSDDLVRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALDHASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDHASVDVEVLGDRVKDANRRGRRLLGK 293

[Close]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL
Match: A0A5D3BJM2 (SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001190 PE=4 SV=1)

HSP 1 Score: 511 bits (1317), Expect = 6.68e-182
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGS+K H RIGKHSSTPNPFESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS S+TWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           P+ISS++ VRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQK EVEK+KQDDALSD
Sbjct: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALD ASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

[Close]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL
Match: A0A1S3BI33 (SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE=4 SV=1)

HSP 1 Score: 511 bits (1317), Expect = 6.68e-182
Identity = 259/293 (88.40%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKHSSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNPFDDDHG 60
           MFGS+K H RIGKHSSTPNPFESDD VKAKQN VS+QRTSSDPSL+TE F SNPFDDDHG
Sbjct: 1   MFGSKKVHFRIGKHSSTPNPFESDDDVKAKQNLVSSQRTSSDPSLLTEKFSSNPFDDDHG 60

Query: 61  HAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVNGCLKIAEEI 120
           HAPSSS S+TWSERNKYKNDFRDSGGIENQSVQELENYAVYK+EETTKAVNGCLKIAEEI
Sbjct: 61  HAPSSSSSITWSERNKYKNDFRDSGGIENQSVQELENYAVYKAEETTKAVNGCLKIAEEI 120

Query: 121 RDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180
           R+DATQTLVALH QGEQI RTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG
Sbjct: 121 REDATQTLVALHHQGEQITRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWKPKKTRPISG 180

Query: 181 PVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTKAAYAEPTSAIQKDEVEKVKQDDALSD 240
           P+ISS++ VRRKGNH+EQKE+LGLT HK+RT+TKAA+AEPT+AIQK EVEK+KQDDALSD
Sbjct: 181 PIISSDE-VRRKGNHLEQKERLGLTHHKERTSTKAAFAEPTNAIQKVEVEKMKQDDALSD 240

Query: 241 MSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRLLGK 293
           MSDILV+LKEMAVDMG E+DRQN ALD ASVDVEV+GDRVKDANRRGRRLLGK
Sbjct: 241 MSDILVQLKEMAVDMGSEMDRQNVALDRASVDVEVLGDRVKDANRRGRRLLGK 292

[Close]

BLAST of Cp4.1LG20g00650 vs. TAIR 10
Match: AT5G61210.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 33 )

HSP 1 Score: 315.8 bits (808), Expect = 3.5e-86
Identity = 175/303 (57.76%), Postives = 220/303 (72.61%), Query Frame = 0


Query: 1   MFGSRKAHLRIGKH------SSTPNPFESDDGVKAKQNQVSAQRTSSDPSLVTENFGSNP 60
           MFG RK+   + KH      SS PNPF+SDD    K     ++RT+S+PSL      +NP
Sbjct: 1   MFGLRKSPANLPKHNSVDLKSSKPNPFDSDDESDNKHTLNPSKRTTSEPSLADM---TNP 60

Query: 61  FDDDH---GHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVYKSEETTKAVN 120
           F  +    G + SS  SL  + + +YKN+FRDSGGIENQSVQELE YAVYK+EETTK+V 
Sbjct: 61  FGGERVQKGDSSSSKQSLFSNSKYQYKNNFRDSGGIENQSVQELEGYAVYKAEETTKSVQ 120

Query: 121 GCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGNLGGMFSKTWK 180
           GCLK+AE+IR DAT+TLV LH QGEQI RTH  A +ID+DLSRGEKLLG+LGGMFSKTWK
Sbjct: 121 GCLKVAEDIRSDATRTLVMLHDQGEQITRTHHKAVEIDHDLSRGEKLLGSLGGMFSKTWK 180

Query: 181 PKKTRPISGPVISSEDLVRRKGNHMEQKEKLGL-TRHKDRTNTKAAYAEPTSAIQKDEVE 240
           PKKTRPI+GPV++ +D   R+ NH+E++EKLGL +  + ++ T+    E   A Q+ E+E
Sbjct: 181 PKKTRPINGPVVTRDDSPTRRVNHLEKREKLGLNSAPRGQSRTREPLPESADAYQRVEME 240

Query: 241 KVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRVKDANRRGRRL 294
           K KQDD LSD+SDIL ELK MAVDMG EI++QN  LDH   DV+ +  RV+ +N+RGRRL
Sbjct: 241 KAKQDDGLSDLSDILGELKNMAVDMGSEIEKQNKGLDHLHDDVDELNFRVQQSNQRGRRL 300

[Close]

BLAST of Cp4.1LG20g00650 vs. TAIR 10
Match: AT1G13890.1 (soluble N-ethylmaleimide-sensitive factor adaptor protein 30 )

HSP 1 Score: 270.4 bits (690), Expect = 1.7e-72
Identity = 147/259 (56.76%), Postives = 186/259 (71.81%), Query Frame = 0


Query: 35  SAQRTSSDPSLVTENFGSNPFDDDHGHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQE 94
           + +RTSS+P L+T +     FDDD                +KYKN F DSGG+++Q+ +E
Sbjct: 27  AGRRTSSEPILITPD-----FDDD----------------DKYKNGFNDSGGLQSQTTEE 86

Query: 95  LENYAVYKSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSR 154
           LE YAVYK+EETTK VN CLKIAE+IR D  +TL  LHQQGEQINRTH +A D+D DLSR
Sbjct: 87  LEKYAVYKAEETTKGVNNCLKIAEDIRSDGARTLEMLHQQGEQINRTHEMAVDMDKDLSR 146

Query: 155 GEKLLGNLGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRHKDRTNTK 214
           GEKLL NLGGMFSK WKPKKT+ I+GP+I+ +   ++  NH E++EKLGL   K R++++
Sbjct: 147 GEKLLNNLGGMFSKPWKPKKTKNITGPMITPDKPSKKSENHKEEREKLGLGA-KGRSSSQ 206

Query: 215 AAYAEPTSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVE 274
            A  +PT+A+QK E EK KQDD LSD+SDIL +LK MAVDMG EID+QN ALDH   DV+
Sbjct: 207 PALDQPTNALQKVEQEKAKQDDGLSDLSDILGDLKSMAVDMGSEIDKQNKALDHLGDDVD 263

Query: 275 VIGDRVKDANRRGRRLLGK 294
            +  RV+ AN+R R LL K
Sbjct: 267 ELNSRVQGANQRARHLLSK 263

[Close]

BLAST of Cp4.1LG20g00650 vs. TAIR 10
Match: AT5G07880.1 (synaptosomal-associated protein SNAP25-like 29 )

HSP 1 Score: 217.6 bits (553), Expect = 1.3e-56
Identity = 125/253 (49.41%), Postives = 166/253 (65.61%), Query Frame = 0


Query: 42  DPSLVTENFGSNPFDDDHGHAPSSSGSLTWSERNKYKNDFRDSGGIENQSVQELENYAVY 101
           D     ++F  NPFDDD                 ++ +  + SGG ENQ+VQELE+YAVY
Sbjct: 13  DEKEAAKSFSLNPFDDDDDDK---------EVEKRFTSSLKPSGGKENQTVQELESYAVY 72

Query: 102 KSEETTKAVNGCLKIAEEIRDDATQTLVALHQQGEQINRTHMVAADIDYDLSRGEKLLGN 161
            SEETTK V GCLK+AEEIR DA++TLV L++QG+QI RTH    D+D+ LSRGEK+LG 
Sbjct: 73  NSEETTKTVQGCLKVAEEIRCDASKTLVMLNEQGDQITRTHQKTVDLDHHLSRGEKILGR 132

Query: 162 LGGMFSKTWKPKKTRPISGPVISSEDLVRRKGNHMEQKEKLGLTRH-KDRTNTKAAYAEP 221
           LGG+FS+TWKPKK+R I+GPVI+  D  +RK   ++ +EKLGL    K ++ T     E 
Sbjct: 133 LGGVFSRTWKPKKSRSITGPVITKGDSPKRKVIDLKTREKLGLNPSLKPKSKT---LPEA 192

Query: 222 TSAIQKDEVEKVKQDDALSDMSDILVELKEMAVDMGCEIDRQNHALDHASVDVEVIGDRV 281
             A QK ++   KQD+AL+D+S +L ELK MAVDMG  I+RQ + LDH   + + +  RV
Sbjct: 193 VDAYQKTQI--AKQDEALTDLSALLGELKNMAVDMGTAIERQTNELDHLQDNADELNYRV 251

Query: 282 KDANRRGRRLLGK 294
           K +N+R R LL K
Sbjct: 253 KQSNQRARYLLRK 251

The following BLAST results are available for this feature:

BLAST of Cp4.1LG20g00650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7P9	5.0e-85	57.76	SNAP25 homologous protein SNAP33 OS=Arabidopsis thaliana OX=3702 GN=SNAP33 PE=1 ...	[more]
Q9LMG8	2.4e-71	56.76	Putative SNAP25 homologous protein SNAP30 OS=Arabidopsis thaliana OX=3702 GN=SNA...	[more]
Q9SD96	1.8e-55	49.41	SNAP25 homologous protein SNAP29 OS=Arabidopsis thaliana OX=3702 GN=SNAP29 PE=2 ...	[more]
Q17QQ3	4.0e-10	27.36	Synaptosomal-associated protein 25 OS=Bos taurus OX=9913 GN=SNAP25 PE=1 SV=1	[more]
P60878	4.0e-10	27.36	Synaptosomal-associated protein 25 OS=Gallus gallus OX=9031 GN=SNAP25 PE=1 SV=1	[more]

BLAST of Cp4.1LG20g00650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520322.1	1.76e-207	100.00	SNAP25 homologous protein SNAP33-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924192.1	8.04e-204	98.98	SNAP25 homologous protein SNAP33-like [Cucurbita moschata]	[more]
XP_023000657.1	7.08e-198	96.25	SNAP25 homologous protein SNAP33-like [Cucurbita maxima]	[more]
XP_038894894.1	4.89e-189	90.78	SNAP25 homologous protein SNAP33-like [Benincasa hispida] >XP_038894895.1 SNAP25...	[more]
KAG6584421.1	3.42e-184	98.91	SNAP25-likeous protein SNAP33, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cp4.1LG20g00650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E891	3.89e-204	98.98	SNAP25 homologous protein SNAP33-like OS=Cucurbita moschata OX=3662 GN=LOC111431...	[more]
A0A6J1KE92	3.43e-198	96.25	SNAP25 homologous protein SNAP33-like OS=Cucurbita maxima OX=3661 GN=LOC11149502...	[more]
A0A0A0LQ81	2.53e-184	88.40	t-SNARE coiled-coil homology domain-containing protein OS=Cucumis sativus OX=365...	[more]
A0A5D3BJM2	6.68e-182	88.40	SNAP25-like proteinous protein SNAP33-like OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BI33	6.68e-182	88.40	SNAP25 homologous protein SNAP33-like OS=Cucumis melo OX=3656 GN=LOC103490217 PE...	[more]

BLAST of Cp4.1LG20g00650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61210.1	3.5e-86	57.76	soluble N-ethylmaleimide-sensitive factor adaptor protein 33	[more]
AT1G13890.1	1.7e-72	56.76	soluble N-ethylmaleimide-sensitive factor adaptor protein 30	[more]
AT5G07880.1	1.3e-56	49.41	synaptosomal-associated protein SNAP25-like 29	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000727	Target SNARE coiled-coil homology domain	SMART	SM00397	tSNARE_6	coord: 223..290 e-value: 0.029 score: 23.5 coord: 95..162 e-value: 3.0 score: 10.1
IPR000727	Target SNARE coiled-coil homology domain	PROSITE	PS50192	T_SNARE	coord: 233..290 score: 13.40722
None	No IPR available	GENE3D	1.20.5.110		coord: 86..164 e-value: 3.9E-19 score: 70.4
None	No IPR available	GENE3D	1.20.5.110		coord: 207..292 e-value: 9.5E-19 score: 69.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..72
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 16..51
None	No IPR available	PANTHER	PTHR19305:SF30	TARGET SNARE COILED-COIL DOMAIN-CONTAINING PROTEIN-RELATED	coord: 1..293
None	No IPR available	PANTHER	PTHR19305	SYNAPTOSOMAL ASSOCIATED PROTEIN	coord: 1..293
None	No IPR available	CDD	cd15841	SNARE_Qc	coord: 233..289 e-value: 2.72056E-11 score: 56.0276
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 227..291
None	No IPR available	SUPERFAMILY	58038	SNARE fusion complex	coord: 90..160
IPR044766	NPSN/SNAP25-like, N-terminal SNARE domain	CDD	cd15861	SNARE_SNAP25N_23N_29N_SEC9N	coord: 97..161 e-value: 2.88474E-19 score: 77.9987

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG20g00650.1	Cp4.1LG20g00650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0061025	membrane fusion
cellular_component	GO:0005886	plasma membrane
cellular_component	GO:0031201	SNARE complex
molecular_function	GO:0005484	SNAP receptor activity