Cp4.1LG20g00440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG20g00440 vs. ExPASy Swiss-Prot
Match: Q9LD37 (Probable cyclic nucleotide-gated ion channel 20, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CNGC20 PE=2 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 1.7e-241
Identity = 433/724 (59.81%), Postives = 548/724 (75.69%), Query Frame = 0

Query: 55  EKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSS-GIYVSDAKLVGYTGPLR 114
           E DD PML     P+ D    +   ++ S +RS SLS PTSS   + +   ++GYTGPLR
Sbjct: 6   ENDDIPML-----PISDPSSRTRARAFTSRSRSVSLSNPTSSIEGFDTSTVVLGYTGPLR 65

Query: 115 IERKSPFLE-SGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHHYA 174
            +R+ P ++ SG   + RK E      P  ++S       +++PSF   +  + +   + 
Sbjct: 66  TQRRPPLVQMSGPLTSTRKHEPLFLPHPS-SDSVGVSSQPERYPSFAALEHKNSSEDEFV 125

Query: 175 GRNEHLIKSGQLGVCNDPFCITCPT-YNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWAV 234
            ++ +L++SGQLG+CNDP+C TCP+ YN KA+Q  +SR+  +FD  FH  LY + KGWA 
Sbjct: 126 LKHANLLRSGQLGMCNDPYCTTCPSYYNRKAAQIPTSRVSALFDSTFHNALYDDAKGWAR 185

Query: 235 KFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFI 294
           +F S    +  Y+PG+MNPHAK VQ W KFF +SCLLAIF+DPLFFF++ V    KCI I
Sbjct: 186 RFAS---SVNRYLPGIMNPHAKEVQTWTKFFALSCLLAIFIDPLFFFLIKVQEQNKCIMI 245

Query: 295 NWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNF 354
           +WPMT   V  RS+TD ++ +++LLQFRLAYVA E+ VVGAG+LV HPKKIA +YLKG F
Sbjct: 246 DWPMTKAFVAVRSVTDVIFTMNILLQFRLAYVARESTVVGAGQLVSHPKKIALHYLKGKF 305

Query: 355 FIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPS 414
           F+D+ +V+PLPQI++  I+P  LG SGAN+AKNLLR AV+ QYIP+LYR LP LAGQ+P+
Sbjct: 306 FLDLFIVMPLPQILILWIIPAHLGASGANYAKNLLRAAVLFQYIPKLYRLLPFLAGQTPT 365

Query: 415 GFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCG 474
           GF+FE+AWANFVINLLTFMLAGH+VGS WYLFGLQ VN+C R+AC   G   C + IDCG
Sbjct: 366 GFIFESAWANFVINLLTFMLAGHVVGSCWYLFGLQRVNQCLRNACGNFGRE-CQDLIDCG 425

Query: 475 HGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFWG 534
           +G+ +      L R  WK N +A ACF ++GF YGIYL+AVNLT  SNL TRY+YSLFWG
Sbjct: 426 NGNSS-----VLVRATWKDNASANACFQEDGFPYGIYLKAVNLTNHSNLFTRYSYSLFWG 485

Query: 535 FQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRR 594
           FQQISTLAGNQVPSY++ EV FTMGIIGLGLLLFALLIGNMQ FLQALG+R LEM+LRRR
Sbjct: 486 FQQISTLAGNQVPSYFLGEVFFTMGIIGLGLLLFALLIGNMQNFLQALGKRNLEMTLRRR 545

Query: 595 DVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNK 654
           DVEQWM HRRLP+ +RR+VR+AER+NWA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ K
Sbjct: 546 DVEQWMSHRRLPDGIRRRVREAERFNWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKK 605

Query: 655 VRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIVV 714
           VRIF+LM+ EPILDAIRERL+QRTYI  S +   G ++EKMVFIVRG+MES +  +G V+
Sbjct: 606 VRIFSLMD-EPILDAIRERLKQRTYIGSSTVLHRGGLVEKMVFIVRGEMES-IGEDGSVL 665

Query: 715 PLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEE 774
           PL EGDVCGEELLTWCLE SS+N D  R  +P++ L+S+R V+C TNVEAFSL  ADLE+
Sbjct: 666 PLYEGDVCGEELLTWCLERSSVNPDGTRIRMPSKGLLSSRNVRCVTNVEAFSLSVADLED 712

Query: 775 VTSM 776
           VTS+
Sbjct: 726 VTSL 712

BLAST of Cp4.1LG20g00440 vs. ExPASy Swiss-Prot
Match: Q9LDR2 (Putative cyclic nucleotide-gated ion channel 19 OS=Arabidopsis thaliana OX=3702 GN=CNGC19 PE=3 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.7e-204
Identity = 387/700 (55.29%), Postives = 494/700 (70.57%), Query Frame = 0

Query: 80  SYASLARSSSLSIPT-SSGIYVSDAKLVGYTGPLRIERKSPFL--ESGSKYTRRKPEKSS 139
           ++ S  RS SLS P+ S   + +    +GYTGPLR +R  P L   SG  ++ R+ E   
Sbjct: 6   TFTSRNRSVSLSNPSFSIDGFDNSTVTLGYTGPLRTQRIRPPLVQMSGPIHSTRRTEPLF 65

Query: 140 QSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHHYAGRNEHLIKSGQLGVCNDPFCITCP 199
             SP   ES  +    D  P      E D+       +N +L++SGQLG+CNDP+C TCP
Sbjct: 66  SPSP--QESPDSSSTVDVPP------EDDFVF-----KNANLLRSGQLGMCNDPYCTTCP 125

Query: 200 T-YNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWAVKFRSVWCFLLPYIPGVMNPHAKVV 259
           + YN +A+Q  +SR+       F   LYG+ +GWA +F S    +   +PG+MNPH+K V
Sbjct: 126 SYYNRQAAQLHTSRVSA---SRFRTVLYGDARGWAKRFAS---SVRRCLPGIMNPHSKFV 185

Query: 260 QQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMTDFVYFLHML 319
           Q W +    S L+AIF+DPLFFF+L +    KCI I+W  T  LV  RS+TD ++F+++L
Sbjct: 186 QVWTRVLAFSSLVAIFIDPLFFFLLLIQQDNKCIAIDWRATKVLVSLRSITDLIFFINIL 245

Query: 320 LQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIVFLILPNSLG 379
           LQFRLAYVAPE+R+VGAG+LVDHP+KIA++Y +G F +D+ +V P+PQI++  I+P  LG
Sbjct: 246 LQFRLAYVAPESRIVGAGQLVDHPRKIARHYFRGKFLLDMFIVFPIPQIMILRIIPLHLG 305

Query: 380 TSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPSGFVFETAWANFVINLLTFMLAGHI 439
           T      K +LR  V+ QYIP+LYR LPLLAGQ+ +GF+FE+AWANFVINLLTFMLAGH 
Sbjct: 306 TRREESEKQILRATVLFQYIPKLYRLLPLLAGQTSTGFIFESAWANFVINLLTFMLAGHA 365

Query: 440 VGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRRDQWKSNENAT 499
           VGS WYL  LQ V +C  +A N +        IDC  G  A       +RD W+ N +  
Sbjct: 366 VGSCWYLSALQRVKKCMLNAWNISADE-RRNLIDCARGSYASKS----QRDLWRDNASVN 425

Query: 500 ACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFWGFQQISTLAGNQVPSYYVYEVLFTM 559
           ACF + G+ YGIYL+AVNLT ES+  TR++YSL+WGFQQISTLAGN  PSY V EV FTM
Sbjct: 426 ACFQENGYTYGIYLKAVNLTNESSFFTRFSYSLYWGFQQISTLAGNLSPSYSVGEVFFTM 485

Query: 560 GIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRLPESLRRKVRQAER 619
           GIIGLGLLLFA LIGNM  FLQ+L RRR+EM LR+RDVEQWM HRRLPE +R++VR+ ER
Sbjct: 486 GIIGLGLLLFARLIGNMHNFLQSLDRRRMEMMLRKRDVEQWMSHRRLPEDIRKRVREVER 545

Query: 620 YNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKVRIFALMNYEPILDAIRERLRQRT 679
           Y WA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KVRIF+LM+ E +LD+IRERL+QRT
Sbjct: 546 YTWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKVRIFSLMD-ESVLDSIRERLKQRT 605

Query: 680 YIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIVVPLSEGDVCGEELLTWCLEHSSINR 739
           YI  S +     ++EKMVFIVRG+MES +  +G V+PLSEGDVCGEELLTWCL  SSIN 
Sbjct: 606 YIRSSTVLHHRGLVEKMVFIVRGEMES-IGEDGSVLPLSEGDVCGEELLTWCL--SSINP 665

Query: 740 DMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTSM 776
           D  R  +P + LVSNR V+C TNVEAFSL  ADLE+VTS+
Sbjct: 666 DGTRIKMPPKGLVSNRNVRCVTNVEAFSLSVADLEDVTSL 677

BLAST of Cp4.1LG20g00440 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.4e-104
Identity = 220/538 (40.89%), Postives = 318/538 (59.11%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           +++P    +Q+WNK FV++C++A+ LDPLFF+V  +   KKC+ I+  M  T  V RS T
Sbjct: 84  ILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFT 143

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y LH++ QFR  ++AP +RV G G LV+  ++IAK YL  +F ID+L VLPLPQ+++
Sbjct: 144 DVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVI 203

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQS-PSGFVFETAWANFVIN 426
            +I+P+  G+S  N  KN+L+  V  QYIPR  R  PL    +  SG + ETAWA    N
Sbjct: 204 LIIIPHMRGSSSLN-TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFN 263

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRR 486
           L  +MLA H+ G+ WYLF ++    C++ AC R       + + C      G     L  
Sbjct: 264 LFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAF--LNE 323

Query: 487 DQWKSNENATACFAKEGFDYGIYLQAV--NLTTESNLLTRYTYSLFWGFQQISTLAGNQV 546
                  N T       FD+GI+L A+   +    +   ++ Y  +WG Q +S+L  N  
Sbjct: 324 SCPIQTPNTTL------FDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLK 383

Query: 547 PSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRLP 606
            S Y++E+ F + I   GL+LF+ LIGNMQ +LQ+   R  EM ++RRD EQWM HR LP
Sbjct: 384 TSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLP 443

Query: 607 ESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMNYEP 666
           E+LR+++R+ E+Y W  TRGV+EE +  NLP+DL+R+I+RHL    + +V +F  M+ E 
Sbjct: 444 ENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMD-EQ 503

Query: 667 ILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV-----VPLSEGD 726
           +LDA+ +RL+   Y E S I   GD +++M+FI+RGK+ +     G         L  GD
Sbjct: 504 LLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGD 563

Query: 727 VCGEELLTWCLE-HSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTS 775
            CGEELLTW L+ HSS N       +P    +S RTV+    VEAF+L+A DL+ V S
Sbjct: 564 FCGEELLTWALDPHSSSN-------LP----ISTRTVRALMEVEAFALKADDLKFVAS 600

BLAST of Cp4.1LG20g00440 vs. ExPASy Swiss-Prot
Match: Q9FXH6 (Putative cyclic nucleotide-gated ion channel 8 OS=Arabidopsis thaliana OX=3702 GN=CNGC8 PE=3 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 3.7e-103
Identity = 233/543 (42.91%), Postives = 325/543 (59.85%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           + +P  K +  WN+ FVISC+LA+ +DPLFF++  V   K CI I+  +  T    R++ 
Sbjct: 97  IFDPQDKTLLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTII 156

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y   M LQFR AY+AP +RV G GELV  P KIA+ YL   F +D L VLPLPQI V
Sbjct: 157 DVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAV 216

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVIN 426
           +  L  S GT      K  L   V+ QYIPR  RF+PL +  +  +G   E AWA     
Sbjct: 217 WKFLHGSKGTD-VLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYY 276

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACN-RTGSTFCLEYIDCGHGDEAGYDIQDLR 486
           LL +MLA HI G+ WY+  ++  + C R AC  +     C++ + CG    +  +   ++
Sbjct: 277 LLWYMLASHITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYCGSKLMSSRETDWIK 336

Query: 487 R--DQWKSNENATACFAKEGFDYGIYLQAVN--LTTESNLLTRYTYSLFWGFQQISTLAG 546
              D +K+N +A +  +K  F+YGIY QAV+  + + +   +++ Y L+WG Q +STL  
Sbjct: 337 SVPDLFKNNCSAKSDESK--FNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQ 396

Query: 547 NQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHR 606
               S Y  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR
Sbjct: 397 GLQTSTYPGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHR 456

Query: 607 RLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMN 666
            LP++LR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA M+
Sbjct: 457 SLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMD 516

Query: 667 YEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMES-RVDG-------NGIVV 726
            E +LDAI ERL+   Y E + I   GD + +M+FI+RG++ES   DG        G+  
Sbjct: 517 -ERLLDAICERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRGL-- 576

Query: 727 PLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEE 775
            L EGD CGEELLTW L+  + +       +P+    S RTVK  T VEAF+L A +L+ 
Sbjct: 577 -LKEGDFCGEELLTWALDPKAGSN------LPS----STRTVKALTEVEAFALEAEELKF 622

BLAST of Cp4.1LG20g00440 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 2.0e-101
Identity = 224/539 (41.56%), Postives = 322/539 (59.74%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           + +P  K +   NK FV SC+L++F+DP FF++  ++   KC+ I+  +  T    R+  
Sbjct: 88  IFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRTFI 147

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y  HM LQ R AY+AP +RV G GELV  P +IAK YL+  F ID L VLPLPQI+V
Sbjct: 148 DVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQIVV 207

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPSGFVF-ETAWANFVIN 426
           +  L +S G S     K  L   V+ QYIPR  R LPL +    +  VF ETAWA     
Sbjct: 208 WRFLQSSNG-SDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAYY 267

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRR 486
           LL +MLA HIVG+ WYL  L+  + C+++AC   G+    +++ CG+ +  GY + +  +
Sbjct: 268 LLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNC-STDFLYCGNQNMDGYAVWNRAK 327

Query: 487 DQ-WKSNENATACFAKEGFDYGIYLQAVN--LTTESNLLTRYTYSLFWGFQQISTLAGNQ 546
           +   KS   A        FD+GIY QA++  + +  N + +Y Y L+WG Q +STL    
Sbjct: 328 ESVLKSKCRADLDDNNPPFDFGIYTQALSSGIVSSQNFIVKYCYCLWWGLQNLSTLGQGL 387

Query: 547 VPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRL 606
             S Y  E++F++ +   GL+LFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR L
Sbjct: 388 ETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRML 447

Query: 607 PESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMNYE 666
           P+ LR +VR+ ++Y W  TRGV+EE + +NLP+DL+R+I+RHL    V +V +F  M+ +
Sbjct: 448 PQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMD-D 507

Query: 667 PILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV-----VPLSEG 726
            +LDAI  RL+   + E + +   GD +++M+FI+RG++ES     G         L EG
Sbjct: 508 KLLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEG 567

Query: 727 DVCGEELLTWCLE-HSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTS 775
           + CGEELLTW L+  S +N       +P+    S RTVK  T VEAF+L + +L+ V S
Sbjct: 568 EFCGEELLTWALDPKSGVN-------LPS----STRTVKALTEVEAFALTSEELKFVAS 612

BLAST of Cp4.1LG20g00440 vs. NCBI nr
Match: XP_023519575.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1453 bits (3762), Expect = 0.0
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH
Sbjct: 61  PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. NCBI nr
Match: XP_023519574.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1453 bits (3762), Expect = 0.0
Identity = 725/725 (100.00%), Postives = 725/725 (100.00%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH
Sbjct: 61  PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. NCBI nr
Match: KAG6584429.1 (putative cyclic nucleotide-gated ion channel 20, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1440 bits (3727), Expect = 0.0
Identity = 720/725 (99.31%), Postives = 720/725 (99.31%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLT KFPSFKTND  DWAI H
Sbjct: 61  PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTGKFPSFKTNDASDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. NCBI nr
Match: KAG7020017.1 (putative cyclic nucleotide-gated ion channel 20, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1439 bits (3724), Expect = 0.0
Identity = 717/725 (98.90%), Postives = 721/725 (99.45%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIE+KSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTN E DWAI H
Sbjct: 61  PLRIEKKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNGESDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSM+DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMSDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQY+PRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYVPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. NCBI nr
Match: XP_022923964.1 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucurbita moschata] >XP_022923965.1 probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 1437 bits (3719), Expect = 0.0
Identity = 718/725 (99.03%), Postives = 719/725 (99.17%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYT RKPEKSSQSSPGVTESKTAEPLT KFPSFKTND  DWAI H
Sbjct: 61  PLRIERKSPFLESGSKYTLRKPEKSSQSSPGVTESKTAEPLTGKFPSFKTNDASDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. ExPASy TrEMBL
Match: A0A6J1EDG0 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431524 PE=4 SV=1)

HSP 1 Score: 1437 bits (3719), Expect = 0.0
Identity = 718/725 (99.03%), Postives = 719/725 (99.17%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYT RKPEKSSQSSPGVTESKTAEPLT KFPSFKTND  DWAI H
Sbjct: 61  PLRIERKSPFLESGSKYTLRKPEKSSQSSPGVTESKTAEPLTGKFPSFKTNDASDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. ExPASy TrEMBL
Match: A0A6J1E859 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431524 PE=4 SV=1)

HSP 1 Score: 1437 bits (3719), Expect = 0.0
Identity = 718/725 (99.03%), Postives = 719/725 (99.17%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLESGSKYT RKPEKSSQSSPGVTESKTAEPLT KFPSFKTND  DWAI H
Sbjct: 61  PLRIERKSPFLESGSKYTLRKPEKSSQSSPGVTESKTAEPLTGKFPSFKTNDASDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALMNYEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMNYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. ExPASy TrEMBL
Match: A0A6J1KKW9 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495122 PE=4 SV=1)

HSP 1 Score: 1416 bits (3666), Expect = 0.0
Identity = 707/725 (97.52%), Postives = 716/725 (98.76%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLS+TGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSNTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLE GSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDE DWAI H
Sbjct: 61  PLRIERKSPFLEIGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDESDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           +AGRNEHLIKSGQLGVCNDPFCITCPTYNSK+S+QKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 FAGRNEHLIKSGQLGVCNDPFCITCPTYNSKSSKQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAV+AQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVIAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTG+T CL+YIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGNTSCLDYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAG    DLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGNHSPDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALM+YEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMDYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. ExPASy TrEMBL
Match: A0A6J1KJA3 (probable cyclic nucleotide-gated ion channel 20, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495122 PE=4 SV=1)

HSP 1 Score: 1416 bits (3666), Expect = 0.0
Identity = 707/725 (97.52%), Postives = 716/725 (98.76%), Query Frame = 0

Query: 51  MASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 110
           MASFEKDDTPMLS+TGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG
Sbjct: 1   MASFEKDDTPMLSNTGPPLLDEQLGSYFPSYASLARSSSLSIPTSSGIYVSDAKLVGYTG 60

Query: 111 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 170
           PLRIERKSPFLE GSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDE DWAI H
Sbjct: 61  PLRIERKSPFLEIGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDESDWAIQH 120

Query: 171 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 230
           +AGRNEHLIKSGQLGVCNDPFCITCPTYNSK+S+QKSSRMRGIFDPEFHKDLYGEGKGWA
Sbjct: 121 FAGRNEHLIKSGQLGVCNDPFCITCPTYNSKSSKQKSSRMRGIFDPEFHKDLYGEGKGWA 180

Query: 231 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 290
           VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF
Sbjct: 181 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 240

Query: 291 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 350
           INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN
Sbjct: 241 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 300

Query: 351 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 410
           FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAV+AQYIPRLYRFLPLLAGQSP
Sbjct: 301 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVIAQYIPRLYRFLPLLAGQSP 360

Query: 411 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 470
           SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTG+T CL+YIDC
Sbjct: 361 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGNTSCLDYIDC 420

Query: 471 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 530
           GHGDEAG    DLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW
Sbjct: 421 GHGDEAGNHSPDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 480

Query: 531 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 590
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR
Sbjct: 481 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 540

Query: 591 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 650
           RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN
Sbjct: 541 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 600

Query: 651 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 710
           KVRIFALM+YEPILDAIRERLRQRTYIEGSE+FTAGDIIEKMVFIVRGKMESRVDGNGIV
Sbjct: 601 KVRIFALMDYEPILDAIRERLRQRTYIEGSEVFTAGDIIEKMVFIVRGKMESRVDGNGIV 660

Query: 711 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 770
           VPLSEGDVCGEELLTWCLEHSSINRDMKRH IPAQKLVSNRTVKCSTNVEAFSLRAADLE
Sbjct: 661 VPLSEGDVCGEELLTWCLEHSSINRDMKRHQIPAQKLVSNRTVKCSTNVEAFSLRAADLE 720

Query: 771 EVTSM 775
           EVTSM
Sbjct: 721 EVTSM 725

BLAST of Cp4.1LG20g00440 vs. ExPASy TrEMBL
Match: A0A5A7SXH8 (Putative cyclic nucleotide-gated ion channel 20 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00360 PE=4 SV=1)

HSP 1 Score: 1278 bits (3308), Expect = 0.0
Identity = 651/785 (82.93%), Postives = 702/785 (89.43%), Query Frame = 0

Query: 1   MQLPASSSLHTFTFFFSLL-PWISLKNPVLSSNL-LHLSQSQF----SNPRP---RYLEM 60
           +QLP S +LHTFT  FSLL PW SL +P+LSS L LHLS SQ     S  RP   R+L M
Sbjct: 32  LQLPTSPTLHTFTLSFSLLSPWNSLWHPLLSSLLHLHLSHSQVEFLGSFDRPVWSRFLGM 91

Query: 61  ASFEKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPT-SSGIYVSDAKLVGYTG 120
           A+FEKDD PMLS+T PP+ DEQ+ SYFPSYASL RSSSLSIP  SSG+Y S+A LVG+TG
Sbjct: 92  AAFEKDDIPMLSNTDPPIFDEQMDSYFPSYASLGRSSSLSIPANSSGMYSSEANLVGHTG 151

Query: 121 PLRIERKSPFLESGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHH 180
           PLRIER S F  SGSK T  KP+K SQS PGVTESKTAEPL +KFPSFK  D+PDW+IH+
Sbjct: 152 PLRIERSS-FAVSGSKNTDSKPKKLSQSKPGVTESKTAEPLAEKFPSFKIVDDPDWSIHN 211

Query: 181 YAGRNEHLIKSGQLGVCNDPFCITCPTYNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWA 240
           YAGRNEHLIKSGQLG+CNDPFCITCPTYNSK SQQKSSR+ GIFD +FH DLYG GKGWA
Sbjct: 212 YAGRNEHLIKSGQLGLCNDPFCITCPTYNSKVSQQKSSRISGIFDAKFHSDLYGGGKGWA 271

Query: 241 VKFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIF 300
            KF+S WCFLL YIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFF+L+V    KCI 
Sbjct: 272 GKFQSFWCFLLSYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFLLSVQEKNKCIV 331

Query: 301 INWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGN 360
           I+WP+TTTLVVFRSMTDF+YFLHMLLQFRLAYVAPE+RVVGAGELVDHPKKIA NYLKGN
Sbjct: 332 IDWPLTTTLVVFRSMTDFIYFLHMLLQFRLAYVAPESRVVGAGELVDHPKKIAMNYLKGN 391

Query: 361 FFIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSP 420
           FFID+LVVLPLPQII+FL+LP SLG+SGAN+AKNLLRTAV  QYIPRLYRFLPLLAGQSP
Sbjct: 392 FFIDLLVVLPLPQIIIFLVLPKSLGSSGANYAKNLLRTAVTVQYIPRLYRFLPLLAGQSP 451

Query: 421 SGFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDC 480
           SGFVFETAWANFVINLLTFMLAGHIVGS WYL GLQ V RCFRDACN+TG+  CLEYIDC
Sbjct: 452 SGFVFETAWANFVINLLTFMLAGHIVGSFWYLLGLQRVTRCFRDACNKTGNRSCLEYIDC 511

Query: 481 GHGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFW 540
           GHGDE+     D  RD WK NENATACF+ EGF YGIYLQAVNLTTE ++LTRYTYSLFW
Sbjct: 512 GHGDESRSQFLDPHRDSWKHNENATACFSNEGFGYGIYLQAVNLTTEKSILTRYTYSLFW 571

Query: 541 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRR 600
           GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQ FLQALGRRRLEMSLRR
Sbjct: 572 GFQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQNFLQALGRRRLEMSLRR 631

Query: 601 RDVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVN 660
           RDVEQWMEHRRLPE+LRR+VRQAERYNWASTRGVNEERIFENLPEDLQ+NIRRHLF+FVN
Sbjct: 632 RDVEQWMEHRRLPENLRRQVRQAERYNWASTRGVNEERIFENLPEDLQKNIRRHLFKFVN 691

Query: 661 KVRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV 720
           KVRIFALM+YEPILDAIRERLRQ+TYIEGSE+F+AGD IEKMVFIVRGKMESRVDGNGIV
Sbjct: 692 KVRIFALMDYEPILDAIRERLRQKTYIEGSEVFSAGDTIEKMVFIVRGKMESRVDGNGIV 751

Query: 721 VPLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLE 775
           VPLSEGDVCGEELLTWCLEHSSIN+DMKR  +PA +LVSNRTV+C +NVEAFSLRAADLE
Sbjct: 752 VPLSEGDVCGEELLTWCLEHSSINKDMKRQQVPAPRLVSNRTVRCLSNVEAFSLRAADLE 811

BLAST of Cp4.1LG20g00440 vs. TAIR 10
Match: AT3G17700.1 (cyclic nucleotide-binding transporter 1 )

HSP 1 Score: 837.0 bits (2161), Expect = 1.2e-242
Identity = 433/724 (59.81%), Postives = 548/724 (75.69%), Query Frame = 0

Query: 55  EKDDTPMLSDTGPPLLDEQLGSYFPSYASLARSSSLSIPTSS-GIYVSDAKLVGYTGPLR 114
           E DD PML     P+ D    +   ++ S +RS SLS PTSS   + +   ++GYTGPLR
Sbjct: 6   ENDDIPML-----PISDPSSRTRARAFTSRSRSVSLSNPTSSIEGFDTSTVVLGYTGPLR 65

Query: 115 IERKSPFLE-SGSKYTRRKPEKSSQSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHHYA 174
            +R+ P ++ SG   + RK E      P  ++S       +++PSF   +  + +   + 
Sbjct: 66  TQRRPPLVQMSGPLTSTRKHEPLFLPHPS-SDSVGVSSQPERYPSFAALEHKNSSEDEFV 125

Query: 175 GRNEHLIKSGQLGVCNDPFCITCPT-YNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWAV 234
            ++ +L++SGQLG+CNDP+C TCP+ YN KA+Q  +SR+  +FD  FH  LY + KGWA 
Sbjct: 126 LKHANLLRSGQLGMCNDPYCTTCPSYYNRKAAQIPTSRVSALFDSTFHNALYDDAKGWAR 185

Query: 235 KFRSVWCFLLPYIPGVMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFI 294
           +F S    +  Y+PG+MNPHAK VQ W KFF +SCLLAIF+DPLFFF++ V    KCI I
Sbjct: 186 RFAS---SVNRYLPGIMNPHAKEVQTWTKFFALSCLLAIFIDPLFFFLIKVQEQNKCIMI 245

Query: 295 NWPMTTTLVVFRSMTDFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNF 354
           +WPMT   V  RS+TD ++ +++LLQFRLAYVA E+ VVGAG+LV HPKKIA +YLKG F
Sbjct: 246 DWPMTKAFVAVRSVTDVIFTMNILLQFRLAYVARESTVVGAGQLVSHPKKIALHYLKGKF 305

Query: 355 FIDVLVVLPLPQIIVFLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPS 414
           F+D+ +V+PLPQI++  I+P  LG SGAN+AKNLLR AV+ QYIP+LYR LP LAGQ+P+
Sbjct: 306 FLDLFIVMPLPQILILWIIPAHLGASGANYAKNLLRAAVLFQYIPKLYRLLPFLAGQTPT 365

Query: 415 GFVFETAWANFVINLLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCG 474
           GF+FE+AWANFVINLLTFMLAGH+VGS WYLFGLQ VN+C R+AC   G   C + IDCG
Sbjct: 366 GFIFESAWANFVINLLTFMLAGHVVGSCWYLFGLQRVNQCLRNACGNFGRE-CQDLIDCG 425

Query: 475 HGDEAGYDIQDLRRDQWKSNENATACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFWG 534
           +G+ +      L R  WK N +A ACF ++GF YGIYL+AVNLT  SNL TRY+YSLFWG
Sbjct: 426 NGNSS-----VLVRATWKDNASANACFQEDGFPYGIYLKAVNLTNHSNLFTRYSYSLFWG 485

Query: 535 FQQISTLAGNQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRR 594
           FQQISTLAGNQVPSY++ EV FTMGIIGLGLLLFALLIGNMQ FLQALG+R LEM+LRRR
Sbjct: 486 FQQISTLAGNQVPSYFLGEVFFTMGIIGLGLLLFALLIGNMQNFLQALGKRNLEMTLRRR 545

Query: 595 DVEQWMEHRRLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNK 654
           DVEQWM HRRLP+ +RR+VR+AER+NWA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ K
Sbjct: 546 DVEQWMSHRRLPDGIRRRVREAERFNWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKK 605

Query: 655 VRIFALMNYEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIVV 714
           VRIF+LM+ EPILDAIRERL+QRTYI  S +   G ++EKMVFIVRG+MES +  +G V+
Sbjct: 606 VRIFSLMD-EPILDAIRERLKQRTYIGSSTVLHRGGLVEKMVFIVRGEMES-IGEDGSVL 665

Query: 715 PLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEE 774
           PL EGDVCGEELLTWCLE SS+N D  R  +P++ L+S+R V+C TNVEAFSL  ADLE+
Sbjct: 666 PLYEGDVCGEELLTWCLERSSVNPDGTRIRMPSKGLLSSRNVRCVTNVEAFSLSVADLED 712

Query: 775 VTSM 776
           VTS+
Sbjct: 726 VTSL 712

BLAST of Cp4.1LG20g00440 vs. TAIR 10
Match: AT3G17690.1 (cyclic nucleotide gated channel 19 )

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-205
Identity = 387/700 (55.29%), Postives = 494/700 (70.57%), Query Frame = 0

Query: 80  SYASLARSSSLSIPT-SSGIYVSDAKLVGYTGPLRIERKSPFL--ESGSKYTRRKPEKSS 139
           ++ S  RS SLS P+ S   + +    +GYTGPLR +R  P L   SG  ++ R+ E   
Sbjct: 6   TFTSRNRSVSLSNPSFSIDGFDNSTVTLGYTGPLRTQRIRPPLVQMSGPIHSTRRTEPLF 65

Query: 140 QSSPGVTESKTAEPLTDKFPSFKTNDEPDWAIHHYAGRNEHLIKSGQLGVCNDPFCITCP 199
             SP   ES  +    D  P      E D+       +N +L++SGQLG+CNDP+C TCP
Sbjct: 66  SPSP--QESPDSSSTVDVPP------EDDFVF-----KNANLLRSGQLGMCNDPYCTTCP 125

Query: 200 T-YNSKASQQKSSRMRGIFDPEFHKDLYGEGKGWAVKFRSVWCFLLPYIPGVMNPHAKVV 259
           + YN +A+Q  +SR+       F   LYG+ +GWA +F S    +   +PG+MNPH+K V
Sbjct: 126 SYYNRQAAQLHTSRVSA---SRFRTVLYGDARGWAKRFAS---SVRRCLPGIMNPHSKFV 185

Query: 260 QQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMTDFVYFLHML 319
           Q W +    S L+AIF+DPLFFF+L +    KCI I+W  T  LV  RS+TD ++F+++L
Sbjct: 186 QVWTRVLAFSSLVAIFIDPLFFFLLLIQQDNKCIAIDWRATKVLVSLRSITDLIFFINIL 245

Query: 320 LQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIVFLILPNSLG 379
           LQFRLAYVAPE+R+VGAG+LVDHP+KIA++Y +G F +D+ +V P+PQI++  I+P  LG
Sbjct: 246 LQFRLAYVAPESRIVGAGQLVDHPRKIARHYFRGKFLLDMFIVFPIPQIMILRIIPLHLG 305

Query: 380 TSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPSGFVFETAWANFVINLLTFMLAGHI 439
           T      K +LR  V+ QYIP+LYR LPLLAGQ+ +GF+FE+AWANFVINLLTFMLAGH 
Sbjct: 306 TRREESEKQILRATVLFQYIPKLYRLLPLLAGQTSTGFIFESAWANFVINLLTFMLAGHA 365

Query: 440 VGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRRDQWKSNENAT 499
           VGS WYL  LQ V +C  +A N +        IDC  G  A       +RD W+ N +  
Sbjct: 366 VGSCWYLSALQRVKKCMLNAWNISADE-RRNLIDCARGSYASKS----QRDLWRDNASVN 425

Query: 500 ACFAKEGFDYGIYLQAVNLTTESNLLTRYTYSLFWGFQQISTLAGNQVPSYYVYEVLFTM 559
           ACF + G+ YGIYL+AVNLT ES+  TR++YSL+WGFQQISTLAGN  PSY V EV FTM
Sbjct: 426 ACFQENGYTYGIYLKAVNLTNESSFFTRFSYSLYWGFQQISTLAGNLSPSYSVGEVFFTM 485

Query: 560 GIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRLPESLRRKVRQAER 619
           GIIGLGLLLFA LIGNM  FLQ+L RRR+EM LR+RDVEQWM HRRLPE +R++VR+ ER
Sbjct: 486 GIIGLGLLLFARLIGNMHNFLQSLDRRRMEMMLRKRDVEQWMSHRRLPEDIRKRVREVER 545

Query: 620 YNWASTRGVNEERIFENLPEDLQRNIRRHLFRFVNKVRIFALMNYEPILDAIRERLRQRT 679
           Y WA+TRGVNEE +FEN+P+DLQR+IRRHLF+F+ KVRIF+LM+ E +LD+IRERL+QRT
Sbjct: 546 YTWAATRGVNEELLFENMPDDLQRDIRRHLFKFLKKVRIFSLMD-ESVLDSIRERLKQRT 605

Query: 680 YIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIVVPLSEGDVCGEELLTWCLEHSSINR 739
           YI  S +     ++EKMVFIVRG+MES +  +G V+PLSEGDVCGEELLTWCL  SSIN 
Sbjct: 606 YIRSSTVLHHRGLVEKMVFIVRGEMES-IGEDGSVLPLSEGDVCGEELLTWCL--SSINP 665

Query: 740 DMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTSM 776
           D  R  +P + LVSNR V+C TNVEAFSL  ADLE+VTS+
Sbjct: 666 DGTRIKMPPKGLVSNRNVRCVTNVEAFSLSVADLEDVTSL 677

BLAST of Cp4.1LG20g00440 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 380.9 bits (977), Expect = 2.4e-105
Identity = 220/538 (40.89%), Postives = 318/538 (59.11%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           +++P    +Q+WNK FV++C++A+ LDPLFF+V  +   KKC+ I+  M  T  V RS T
Sbjct: 84  ILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIIDDAKKCLGIDKKMEITASVLRSFT 143

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y LH++ QFR  ++AP +RV G G LV+  ++IAK YL  +F ID+L VLPLPQ+++
Sbjct: 144 DVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIAKRYLSSHFIIDILAVLPLPQMVI 203

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQS-PSGFVFETAWANFVIN 426
            +I+P+  G+S  N  KN+L+  V  QYIPR  R  PL    +  SG + ETAWA    N
Sbjct: 204 LIIIPHMRGSSSLN-TKNMLKFIVFFQYIPRFIRIYPLYKEVTRTSGILTETAWAGAAFN 263

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRR 486
           L  +MLA H+ G+ WYLF ++    C++ AC R       + + C      G     L  
Sbjct: 264 LFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPCISKLLYCDPETAGGNAF--LNE 323

Query: 487 DQWKSNENATACFAKEGFDYGIYLQAV--NLTTESNLLTRYTYSLFWGFQQISTLAGNQV 546
                  N T       FD+GI+L A+   +    +   ++ Y  +WG Q +S+L  N  
Sbjct: 324 SCPIQTPNTTL------FDFGIFLDALQSGVVESQDFPQKFFYCFWWGLQNLSSLGQNLK 383

Query: 547 PSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRLP 606
            S Y++E+ F + I   GL+LF+ LIGNMQ +LQ+   R  EM ++RRD EQWM HR LP
Sbjct: 384 TSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRVKRRDAEQWMSHRLLP 443

Query: 607 ESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMNYEP 666
           E+LR+++R+ E+Y W  TRGV+EE +  NLP+DL+R+I+RHL    + +V +F  M+ E 
Sbjct: 444 ENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLALLMRVPMFEKMD-EQ 503

Query: 667 ILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV-----VPLSEGD 726
           +LDA+ +RL+   Y E S I   GD +++M+FI+RGK+ +     G         L  GD
Sbjct: 504 LLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGGRTGFLNSEYLGAGD 563

Query: 727 VCGEELLTWCLE-HSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTS 775
            CGEELLTW L+ HSS N       +P    +S RTV+    VEAF+L+A DL+ V S
Sbjct: 564 FCGEELLTWALDPHSSSN-------LP----ISTRTVRALMEVEAFALKADDLKFVAS 600

BLAST of Cp4.1LG20g00440 vs. TAIR 10
Match: AT1G19780.1 (cyclic nucleotide gated channel 8 )

HSP 1 Score: 377.5 bits (968), Expect = 2.6e-104
Identity = 233/543 (42.91%), Postives = 325/543 (59.85%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           + +P  K +  WN+ FVISC+LA+ +DPLFF++  V   K CI I+  +  T    R++ 
Sbjct: 97  IFDPQDKTLLLWNRMFVISCILAVSVDPLFFYLPIVDNSKNCIGIDSKLAVTTTTLRTII 156

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y   M LQFR AY+AP +RV G GELV  P KIA+ YL   F +D L VLPLPQI V
Sbjct: 157 DVFYLTRMALQFRTAYIAPSSRVFGRGELVIDPAKIAERYLTRYFIVDFLAVLPLPQIAV 216

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAG-QSPSGFVFETAWANFVIN 426
           +  L  S GT      K  L   V+ QYIPR  RF+PL +  +  +G   E AWA     
Sbjct: 217 WKFLHGSKGTD-VLPTKQALLHIVITQYIPRFVRFIPLTSELKKTAGAFAEGAWAGAAYY 276

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACN-RTGSTFCLEYIDCGHGDEAGYDIQDLR 486
           LL +MLA HI G+ WY+  ++  + C R AC  +     C++ + CG    +  +   ++
Sbjct: 277 LLWYMLASHITGAFWYMLSVERNDTCLRSACKVQPDPKVCVQILYCGSKLMSSRETDWIK 336

Query: 487 R--DQWKSNENATACFAKEGFDYGIYLQAVN--LTTESNLLTRYTYSLFWGFQQISTLAG 546
              D +K+N +A +  +K  F+YGIY QAV+  + + +   +++ Y L+WG Q +STL  
Sbjct: 337 SVPDLFKNNCSAKSDESK--FNYGIYSQAVSSGIVSSTTFFSKFCYCLWWGLQNLSTLGQ 396

Query: 547 NQVPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHR 606
               S Y  EVLF++ I   GLLLFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR
Sbjct: 397 GLQTSTYPGEVLFSIAIAVAGLLLFALLIGNMQTYLQSLTVRLEEMRIKRRDSEQWMHHR 456

Query: 607 RLPESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMN 666
            LP++LR +VR+ ++Y W  TRGV+EE I ++LP+DL+R+I+RHL    V +V +FA M+
Sbjct: 457 SLPQNLRERVRRYDQYKWLETRGVDEENIVQSLPKDLRRDIKRHLCLNLVRRVPLFANMD 516

Query: 667 YEPILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMES-RVDG-------NGIVV 726
            E +LDAI ERL+   Y E + I   GD + +M+FI+RG++ES   DG        G+  
Sbjct: 517 -ERLLDAICERLKPSLYTESTYIVREGDPVNEMLFIIRGRLESVTTDGGRSGFFNRGL-- 576

Query: 727 PLSEGDVCGEELLTWCLEHSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEE 775
            L EGD CGEELLTW L+  + +       +P+    S RTVK  T VEAF+L A +L+ 
Sbjct: 577 -LKEGDFCGEELLTWALDPKAGSN------LPS----STRTVKALTEVEAFALEAEELKF 622

BLAST of Cp4.1LG20g00440 vs. TAIR 10
Match: AT5G57940.1 (cyclic nucleotide gated channel 5 )

HSP 1 Score: 371.7 bits (953), Expect = 1.4e-102
Identity = 224/539 (41.56%), Postives = 322/539 (59.74%), Query Frame = 0

Query: 247 VMNPHAKVVQQWNKFFVISCLLAIFLDPLFFFVLAVHFVKKCIFINWPMTTTLVVFRSMT 306
           + +P  K +   NK FV SC+L++F+DP FF++  ++   KC+ I+  +  T    R+  
Sbjct: 88  IFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRTFI 147

Query: 307 DFVYFLHMLLQFRLAYVAPETRVVGAGELVDHPKKIAKNYLKGNFFIDVLVVLPLPQIIV 366
           D  Y  HM LQ R AY+AP +RV G GELV  P +IAK YL+  F ID L VLPLPQI+V
Sbjct: 148 DVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQIVV 207

Query: 367 FLILPNSLGTSGANHAKNLLRTAVMAQYIPRLYRFLPLLAGQSPSGFVF-ETAWANFVIN 426
           +  L +S G S     K  L   V+ QYIPR  R LPL +    +  VF ETAWA     
Sbjct: 208 WRFLQSSNG-SDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAYY 267

Query: 427 LLTFMLAGHIVGSLWYLFGLQGVNRCFRDACNRTGSTFCLEYIDCGHGDEAGYDIQDLRR 486
           LL +MLA HIVG+ WYL  L+  + C+++AC   G+    +++ CG+ +  GY + +  +
Sbjct: 268 LLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNC-STDFLYCGNQNMDGYAVWNRAK 327

Query: 487 DQ-WKSNENATACFAKEGFDYGIYLQAVN--LTTESNLLTRYTYSLFWGFQQISTLAGNQ 546
           +   KS   A        FD+GIY QA++  + +  N + +Y Y L+WG Q +STL    
Sbjct: 328 ESVLKSKCRADLDDNNPPFDFGIYTQALSSGIVSSQNFIVKYCYCLWWGLQNLSTLGQGL 387

Query: 547 VPSYYVYEVLFTMGIIGLGLLLFALLIGNMQIFLQALGRRRLEMSLRRRDVEQWMEHRRL 606
             S Y  E++F++ +   GL+LFALLIGNMQ +LQ+L  R  EM ++RRD EQWM HR L
Sbjct: 388 ETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRML 447

Query: 607 PESLRRKVRQAERYNWASTRGVNEERIFENLPEDLQRNIRRHL-FRFVNKVRIFALMNYE 666
           P+ LR +VR+ ++Y W  TRGV+EE + +NLP+DL+R+I+RHL    V +V +F  M+ +
Sbjct: 448 PQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMD-D 507

Query: 667 PILDAIRERLRQRTYIEGSEIFTAGDIIEKMVFIVRGKMESRVDGNGIV-----VPLSEG 726
            +LDAI  RL+   + E + +   GD +++M+FI+RG++ES     G         L EG
Sbjct: 508 KLLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEG 567

Query: 727 DVCGEELLTWCLE-HSSINRDMKRHLIPAQKLVSNRTVKCSTNVEAFSLRAADLEEVTS 775
           + CGEELLTW L+  S +N       +P+    S RTVK  T VEAF+L + +L+ V S
Sbjct: 568 EFCGEELLTWALDPKSGVN-------LPS----STRTVKALTEVEAFALTSEELKFVAS 612