Cp4.1LG19g04950 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG19g04950 vs. ExPASy Swiss-Prot
Match: Q9LZS6 (Subtilisin-like protease SBT4.15 OS=Arabidopsis thaliana OX=3702 GN=SBT4.15 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 1.5e-228
Identity = 413/753 (54.85%), Postives = 533/753 (70.78%), Query Frame = 0

Query: 9   VLVLLFWLVASFMIHGS--SHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENK 68
           + +L F LV + +I  +   + ERKPYIVYMG+    +L     ++HHNLL+  IGDE+K
Sbjct: 9   LFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSL-VEAAENHHNLLMTVIGDESK 68

Query: 69  ARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGL-NRR 128
           ARE KI+SYG++ NGF ARL PHEA KLS  EGVVSVF + + ++ TTRSWDFLGL   +
Sbjct: 69  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 128

Query: 129 SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIGAN 188
            KR+  IESNIIV VLDTGI + SPSF+D+G GPPPAKWKG+CVTG NFT CNNKVIGA 
Sbjct: 129 YKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAK 188

Query: 189 YFDL--DNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 248
           YF +  + +      + AD +GHG+HT+ST+AG +V  ASL+G+  GTARGGVPSARIA 
Sbjct: 189 YFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAA 248

Query: 249 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 308
           YKVCW   C+DMD+LA FDEAI+DGVD IS+SIG  ++ FF D  AIGAFHAMK+GILT+
Sbjct: 249 YKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTT 308

Query: 309 CAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQI-- 368
           C+AGN+GP L TV N+APW+MTVAA  +DR+F T  KLGNG   +G S+N F+P+ ++  
Sbjct: 309 CSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYP 368

Query: 369 -----------------ASACDVDALSQSKVKGRIVYCLT---------TSIDYNIESLG 428
                             S C+   L + KV G++VYC              D+ + SL 
Sbjct: 369 LTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLK 428

Query: 429 GTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFV 488
           G G+I  L E TD +   L+ G+ +    G  I  YINSTK+P+A+I+K+KT K+ AP +
Sbjct: 429 GAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAPSI 488

Query: 489 ASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACP 548
           +SF +RGPQ ISPNILKPD++APG++ILAAY+KL+S+TG   D+R +LFS+M+GTSMACP
Sbjct: 489 SSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACP 548

Query: 549 HATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVY 608
           HA AAAAYVKSFHPDWSPAA+KSALMTTATPM+IK  +AE   G+GQINP +A+HPGLVY
Sbjct: 549 HAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRRAIHPGLVY 608

Query: 609 DISLGSYISFLCKEGYNSTTIGLLVG-------SKEYNCSKIKPAKGTDGLNYPTMHKQL 668
           DI+  +Y+ FLCKEGYNST+IGLL G        KEYNC  IK   G+DGLNYP++HKQ+
Sbjct: 609 DITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQV 668

Query: 669 SDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVK 721
           +   + ++ VFYRTVT+V +G S Y A + +P GL V+V P  + F +  E++ FKVV+ 
Sbjct: 669 NSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVID 728

BLAST of Cp4.1LG19g04950 vs. ExPASy Swiss-Prot
Match: Q9LLL8 (Subtilisin-like protease SBT4.14 OS=Arabidopsis thaliana OX=3702 GN=SBT4.14 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.8e-192
Identity = 361/738 (48.92%), Postives = 482/738 (65.31%), Query Frame = 0

Query: 4   HELLFVLVLLFWLVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGD 63
           H LL +++++ W+   +      H  +  YI+Y+GD P  T  T  +  H NLL      
Sbjct: 10  HHLLVLVMVVLWISPRYASAEDEH-AKDFYIIYLGDRPDNTEET--IKTHINLLSSLNIS 69

Query: 64  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGLN 123
           + +A+E K++SY ++FN FAA+L PHEA K+  ME VVSV  ++  ++ TT+SWDF+GL 
Sbjct: 70  QEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLP 129

Query: 124 RRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 183
             +KR+ K E ++I+ VLDTGI  +S SF D G GPPPAKWKG C    NFT CNNK+IG
Sbjct: 130 LTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIG 189

Query: 184 ANYFDLD-NVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIA 243
           A YF  D NV      S  D +GHG+HT+STVAG  V  ASLYG+  GTARG VPSAR+A
Sbjct: 190 AKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLA 249

Query: 244 VYKVCWS-IFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGIL 303
           +YKVCW+   C+DMD+LAGF+ AI DGV+ IS+SIG    D+  DS ++G+FHAM+KGIL
Sbjct: 250 MYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGIL 309

Query: 304 TSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPK--- 363
           T  +AGNDGP   TV N  PWI+TVAA+GIDR F +   LGNGK F+G  I+ FSPK   
Sbjct: 310 TVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKS 369

Query: 364 ---------------NQIASACDVDALSQSKVKGRIVYCLT--TSIDYNIESLGGTGIIE 423
                            +A  C  D+L + KVKG+++ C      ++  I+S GG G I 
Sbjct: 370 YPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAII 429

Query: 424 LLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLSR 483
           + ++  D++ I + P   +    G  I  YINST+   A+I K++ V I APFVASF SR
Sbjct: 430 VSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSR 489

Query: 484 GPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAA 543
           GP   S  +LKPD+AAPGIDILAA+T   SLTG   D+++S F++++GTSMACPH    A
Sbjct: 490 GPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVA 549

Query: 544 AYVKSFHPDWSPAAVKSALMTTATPMKIK-SEDAEFGSGAGQINPTKAVHPGLVYDISLG 603
           AYVKSFHPDW+PAA+KSA++T+A P+  + ++DAEF  G GQINP +A  PGLVYD+   
Sbjct: 550 AYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDI 609

Query: 604 SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFY 663
           SY+ FLC EGYN+TT+  LVG++  +CS I P  G D LNYPT+   L    ++  AVF 
Sbjct: 610 SYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFR 669

Query: 664 RTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILS 719
           R VT+V   +SVY A + +P G+ + V P SL F+KA +++ FKVVVK + M  G +I+S
Sbjct: 670 RRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPG-KIVS 729

BLAST of Cp4.1LG19g04950 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 2.4e-157
Identity = 315/709 (44.43%), Postives = 433/709 (61.07%), Query Frame = 0

Query: 33  YIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAA 92
           YIVYMG       S  +   HH  +L+ +     A ES +H+Y RSFNGFA +L   EA 
Sbjct: 34  YIVYMGRKLEDPDSAHL---HHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAE 93

Query: 93  KLSNMEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSF 152
           K+++MEGVVSVF +  + + TTRSWDFLG      R  ++ESNI+V VLDTGIW  SPSF
Sbjct: 94  KIASMEGVVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSF 153

Query: 153 SDEGYGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDN-VSQFTETSVADTEGHGSHTA 212
            DEG+ PPP KWKG C T  NF  CN K+IGA  + +   +S        DT GHG+HTA
Sbjct: 154 DDEGFSPPPPKWKGTCETSNNF-RCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTA 213

Query: 213 STVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDF 272
           ST AG  V  A+LYGLG GTARGGVP ARIA YKVCW+  CSD D+LA +D+AIADGVD 
Sbjct: 214 STAAGGLVSQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDI 273

Query: 273 ISVSIGSV-AVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATG 332
           IS+S+G      +F D+ AIG+FHA+++GILTS +AGN GP   T  +++PW+++VAA+ 
Sbjct: 274 ISLSVGGANPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAAST 333

Query: 333 IDRRFATAFKLGNGKKFTGFSINTFSPK----------------NQIASACDVDALSQSK 392
           +DR+F T  ++GNG+ F G SINTF  +                   +  C   +++ + 
Sbjct: 334 MDRKFVTQVQIGNGQSFQGVSINTFDNQYYPLVSGRDIPNTGFDKSTSRFCTDKSVNPNL 393

Query: 393 VKGRIVYCLTTSIDYN-IESLGGTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYIN 452
           +KG+IV C  +   +   +SL G   + +     D +    LP +V+ P        YI 
Sbjct: 394 LKGKIVVCEASFGPHEFFKSLDGAAGVLMTSNTRDYADSYPLPSSVLDPNDLLATLRYIY 453

Query: 453 STKDPRAIIYKSKTV-KIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSL 512
           S + P A I+KS T+   +AP V SF SRGP   + +++KPD++ PG++ILAA+  ++ +
Sbjct: 454 SIRSPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV 513

Query: 513 TGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIK-S 572
            G     R +LF++++GTSM+CPH T  A YVK+++P WSPAA+KSALMTTA+PM  + +
Sbjct: 514 GG---IRRNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFN 573

Query: 573 EDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYN-CSKI 632
             AEF  G+G +NP KAV PGLVYD +   Y+ FLC +GYN+  +  + G  +Y+ C+  
Sbjct: 574 PQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITG--DYSACTSG 633

Query: 633 KPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPD 692
              +  D LNYP+    +S P       F RT+T V   AS YRA IS+P GL++ V P+
Sbjct: 634 NTGRVWD-LNYPSFGLSVS-PSQTFNQYFNRTLTSVAPQASTYRAMISAPQGLTISVNPN 693

Query: 693 SLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILIS 720
            L F   G+RK F + V+G        ++SA L W D  H VRS I I+
Sbjct: 694 VLSFNGLGDRKSFTLTVRGSIK---GFVVSASLVWSDGVHYVRSPITIT 728

BLAST of Cp4.1LG19g04950 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 3.2e-157
Identity = 326/711 (45.85%), Postives = 439/711 (61.74%), Query Frame = 0

Query: 33  YIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAA 92
           YIVYMG +P    S      HH  +L  +     A    + SY RSFNGFAA L   E+ 
Sbjct: 33  YIVYMGTLPEIKYSP---PSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQ 92

Query: 93  KLSNMEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSF 152
           KL NM+ VVSVFPS+ H + TTRSWDF+G   +++R    ES++IV V+D+GIW  S SF
Sbjct: 93  KLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESF 152

Query: 153 SDEGYGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTAS 212
            DEG+GPPP KWKG C  G  F ACNNK+IGA ++     ++F + S  D EGHG+HTAS
Sbjct: 153 DDEGFGPPPKKWKGSCKGGLKF-ACNNKLIGARFY-----NKFAD-SARDEEGHGTHTAS 212

Query: 213 TVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFI 272
           T AG+AV+ AS YGL +GTARGGVPSARIA YKVC++  C+D+D+LA FD+AIADGVD I
Sbjct: 213 TAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCFN-RCNDVDILAAFDDAIADGVDVI 272

Query: 273 SVSIGSVAV-DFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGI 332
           S+SI +  V +    S AIG+FHAM +GI+T+ +AGN+GP+  +V NV+PW++TVAA+G 
Sbjct: 273 SISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGT 332

Query: 333 DRRFATAFKLGNGKKFTGFSINTF-----------------SPKNQIASACDVDALSQSK 392
           DR+F     LGNGK  TG S+NTF                 +     A  C    +    
Sbjct: 333 DRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSEL 392

Query: 393 VKGRIVYCLTTSIDYNIESLGGT-GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYIN 452
           VKG+IV C    + Y    L G  G+I       DS+ ++  P + +     K I  YI 
Sbjct: 393 VKGKIVLC-DDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIE 452

Query: 453 STKDPRA-IIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSL 512
           S + P+A I+   + V   AP+V SF SRGP  +  N+LKPD++APG++ILAA++ ++S 
Sbjct: 453 SAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASP 512

Query: 513 TGKI--ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKI- 572
           +  +   D R   +SVM+GTSMACPH    AAYVKSFHPDWSP+A+KSA+MTTATPM + 
Sbjct: 513 SSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLK 572

Query: 573 KSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSK 632
           K+ + EF  G+GQINPTKA  PGLVY++    Y+  LC EG++STT+    G +   CS+
Sbjct: 573 KNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSE 632

Query: 633 IKPAKGTDGLNYPTMHKQLS--DPGSAITAVFYRTVTDVEHGASVYRAN-ISSPVGLSVK 692
               K    LNYPTM   +S  DP       F RTVT+V    S Y+A+ +     L + 
Sbjct: 633 RTEVK---DLNYPTMTTFVSSLDP---FNVTFKRTVTNVGFPNSTYKASVVPLQPELQIS 692

Query: 693 VFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNIL 718
           + P+ L F    E+K F V + G+ ++DG+ + S+++ W D  H VRS I+
Sbjct: 693 IEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVV-WSDGSHSVRSPIV 723

BLAST of Cp4.1LG19g04950 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.7e-150
Identity = 317/730 (43.42%), Postives = 436/730 (59.73%), Query Frame = 0

Query: 16  LVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENKARESKIHSY 75
           LV SF   G    +++ YIVYMG +P+      +   HH  +L  +  E+  ++  + +Y
Sbjct: 17  LVVSFASAGKDDQDKQVYIVYMGALPSRV--DYMPMSHHTSILQDVTGESSIQDRLVRNY 76

Query: 76  GRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGL--NRRSKRNPKIE 135
            RSFNGFAARL   E   L++M+ VVSVFPS+   + TT SW+F+GL   +R+KRNP IE
Sbjct: 77  KRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIE 136

Query: 136 SNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVS 195
           S+ I+ V+D+GI+  S SFS +G+GPPP KWKG C  G NFT CNNK+IGA Y+    + 
Sbjct: 137 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYY-TPKLE 196

Query: 196 QFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVC--WSIF 255
            F E S  D  GHGSHTAS  AG+AV+  S YGLG GT RGGVP+ARIAVYKVC    I 
Sbjct: 197 GFPE-SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIR 256

Query: 256 CSDMDVLAGFDEAIADGVDFISVSIGSVAVDFF-RDSHAIGAFHAMKKGILTSCAAGNDG 315
           C+   +LA FD+AIAD VD I+VS+G+ AV  F  D+ AIGAFHAM KGILT   AGN+G
Sbjct: 257 CTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNG 316

Query: 316 PELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQ---------I 375
           PE  T+ ++APW+ TVAA+ ++R F T   LGNGK   G S+N+F    +          
Sbjct: 317 PERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA 376

Query: 376 ASACDVDA--------LSQSKVKGRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPIL 435
           +S CD  +        L   +VKG+IV C T       +++G    I +     D++ + 
Sbjct: 377 SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASI-VRNPYEDAASVF 436

Query: 436 LLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTV-KIAAPFVASFLSRGPQLISPNILK 495
             P +V+       +  Y+NSTK+P+A + KS+T+    AP VAS+ SRGP  +  +ILK
Sbjct: 437 SFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILK 496

Query: 496 PDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWS 555
           PD+ APG +ILAAY+    +    +D+R+  ++V++GTSM+CPH    AAY+K+FHP WS
Sbjct: 497 PDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWS 556

Query: 556 PAAVKSALMTTATPMKI----KSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCK 615
           P+ ++SA+MTTA PM       +E AEF  GAG ++P  A+HPGLVY+ +   +I+FLC 
Sbjct: 557 PSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCG 616

Query: 616 EGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTVTDVEH 675
             Y    + L+ G    +C+K +    T  LNYP+M  Q+S         F RTVT+V  
Sbjct: 617 FNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPFKVTFRRTVTNVGR 676

Query: 676 GASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDS 719
             + Y+A +     L VKV P  L      E+K F V V G   +    ++SA L W D 
Sbjct: 677 PNATYKAKVVGS-KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK-AENLVSAQLIWSDG 734

BLAST of Cp4.1LG19g04950 vs. NCBI nr
Match: KAG7027209.1 (Subtilisin-like protease SBT4.15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1313 bits (3399), Expect = 0.0
Identity = 668/705 (94.75%), Postives = 672/705 (95.32%), Query Frame = 0

Query: 37  MGDMPAGTLSTTVVDDHHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSN 96
           MGD+PAGTLSTT  DDHHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSN
Sbjct: 1   MGDLPAGTLSTTAADDHHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSN 60

Query: 97  MEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG 156
           +EGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG
Sbjct: 61  VEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG 120

Query: 157 YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAG 216
           YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTE SVADTEGHGSHTASTVAG
Sbjct: 121 YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTEPSVADTEGHGSHTASTVAG 180

Query: 217 SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI 276
           SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI
Sbjct: 181 SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI 240

Query: 277 GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFA 336
           GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRF 
Sbjct: 241 GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFV 300

Query: 337 TAFKLGNGKKFTGFSINTFSPKNQ--------------------IASACDVDALSQSKVK 396
           TAFKLGNGKKFTGFSINTFSP+NQ                     ASACDVDALSQSKVK
Sbjct: 301 TAFKLGNGKKFTGFSINTFSPRNQKYSLTSGAKASNNGTILGNASASACDVDALSQSKVK 360

Query: 397 GRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTK 456
           GRIVYCLTT  DYNIESLGGTGIIELLE QTDSSPILLLPGAVIPPVSGKYIDLYINSTK
Sbjct: 361 GRIVYCLTTFTDYNIESLGGTGIIELLENQTDSSPILLLPGAVIPPVSGKYIDLYINSTK 420

Query: 457 DPRAIIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI 516
           DPRAIIYKSKTVKIAAPFVASF SRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI
Sbjct: 421 DPRAIIYKSKTVKIAAPFVASFSSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI 480

Query: 517 ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF 576
           ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF
Sbjct: 481 ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF 540

Query: 577 GSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGT 636
           GSGAGQINPTKAVHPGLVYDISL SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPA+GT
Sbjct: 541 GSGAGQINPTKAVHPGLVYDISLDSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGT 600

Query: 637 DGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAK 696
           DGLNYPTMHKQLSDPGSAITAVFYRTVT VEHGASVYRANISSPVGLSVKVFPDSLDFAK
Sbjct: 601 DGLNYPTMHKQLSDPGSAITAVFYRTVTHVEHGASVYRANISSPVGLSVKVFPDSLDFAK 660

Query: 697 AGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILISRQ 721
           AGERK FKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILI RQ
Sbjct: 661 AGERKTFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILIYRQ 705

BLAST of Cp4.1LG19g04950 vs. NCBI nr
Match: KAG6595191.1 (Subtilisin-like protease 4.15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1277 bits (3305), Expect = 0.0
Identity = 644/663 (97.13%), Postives = 651/663 (98.19%), Query Frame = 0

Query: 59  DTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWD 118
           D++  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSN+EGVVSVFPSRKHRVVTTRSWD
Sbjct: 6   DSLDSENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWD 65

Query: 119 FLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACN 178
           FLGLNRRSKRNPKIESN+IVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACN
Sbjct: 66  FLGLNRRSKRNPKIESNVIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACN 125

Query: 179 NKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPS 238
           NKVIGANYFDLDNVSQFTE SVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPS
Sbjct: 126 NKVIGANYFDLDNVSQFTEPSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPS 185

Query: 239 ARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKK 298
           ARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKK
Sbjct: 186 ARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKK 245

Query: 299 GILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPK 358
           GILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRF TAFKLGNGKKFTGFSINTFSP+
Sbjct: 246 GILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFVTAFKLGNGKKFTGFSINTFSPR 305

Query: 359 NQIASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILLLPGA 418
           NQ ASAC+VDALSQSKVKGRIVYCLTT  DYNIESLGGTGIIELLEEQTDSSPILLLPGA
Sbjct: 306 NQNASACEVDALSQSKVKGRIVYCLTTFTDYNIESLGGTGIIELLEEQTDSSPILLLPGA 365

Query: 419 VIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAP 478
           VIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASF SRGPQLISPNILKPDLAAP
Sbjct: 366 VIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFSSRGPQLISPNILKPDLAAP 425

Query: 479 GIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKS 538
           GIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKS
Sbjct: 426 GIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKS 485

Query: 539 ALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGL 598
           ALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISL SYISFLCKEGYNSTTIGL
Sbjct: 486 ALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLDSYISFLCKEGYNSTTIGL 545

Query: 599 LVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANIS 658
           LVGSKEYNCSKIKPA+GTDGLNYPTMHKQLSDPGSAITAVFYRTVT VEHGASVYRANIS
Sbjct: 546 LVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITAVFYRTVTHVEHGASVYRANIS 605

Query: 659 SPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILI 718
           SPVGLSVKVFPDSLDFAKAGERK FKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILI
Sbjct: 606 SPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILI 665

Query: 719 SRQ 721
            RQ
Sbjct: 666 YRQ 668

BLAST of Cp4.1LG19g04950 vs. NCBI nr
Match: XP_022962647.1 (subtilisin-like protease SBT4.15 [Cucurbita moschata])

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 643/684 (94.01%), Postives = 648/684 (94.74%), Query Frame = 0

Query: 58  LDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSW 117
           LD+   ENKARESKIHSYGRSFNGFAARLLPHEAAKLSN+EGVVSVFPSRKHRVVTTRSW
Sbjct: 8   LDSYVSENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSW 67

Query: 118 DFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC 177
           +FLGLNRRSKRN KIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC
Sbjct: 68  NFLGLNRRSKRNLKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC 127

Query: 178 NNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP 237
           NNKVIGANYFDLDNVSQFTE SVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP
Sbjct: 128 NNKVIGANYFDLDNVSQFTEPSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP 187

Query: 238 SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK 297
           SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK
Sbjct: 188 SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK 247

Query: 298 KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSP 357
           KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRF TAFKLGN KKFTGFSINTFSP
Sbjct: 248 KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFVTAFKLGNDKKFTGFSINTFSP 307

Query: 358 KNQ--------------------IASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGT 417
           +NQ                     ASACDVDALSQSKVKGRIVYCLTT  DYNIESLGGT
Sbjct: 308 RNQKYSLTSGAKASNNGTILGNASASACDVDALSQSKVKGRIVYCLTTFTDYNIESLGGT 367

Query: 418 GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS 477
           GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS
Sbjct: 368 GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS 427

Query: 478 FLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA 537
           F SRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA
Sbjct: 428 FSSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA 487

Query: 538 TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI 597
           TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI
Sbjct: 488 TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI 547

Query: 598 SLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITA 657
           SL SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPA+GTDGLNYPTMHKQLSDPGSAITA
Sbjct: 548 SLNSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITA 607

Query: 658 VFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTR 717
           VFYRTVT VEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERK FKVVVKGEAMRDGTR
Sbjct: 608 VFYRTVTHVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTR 667

Query: 718 ILSALLEWKDSKHVVRSNILISRQ 721
           ILSALLEWK SKHVVRSNILI RQ
Sbjct: 668 ILSALLEWKGSKHVVRSNILIYRQ 691

BLAST of Cp4.1LG19g04950 vs. NCBI nr
Match: XP_022972607.1 (subtilisin-like protease SBT4.15 [Cucurbita maxima])

HSP 1 Score: 1252 bits (3240), Expect = 0.0
Identity = 637/678 (93.95%), Postives = 645/678 (95.13%), Query Frame = 0

Query: 64  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGLN 123
           ENKARESKIHSYGRSFNGFAARLLPHEAAKLSN+EGVVSVFPSRKHRVVTTRSWDFLGLN
Sbjct: 11  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWDFLGLN 70

Query: 124 RRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 183
            RSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG
Sbjct: 71  HRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 130

Query: 184 ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 243
           ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV
Sbjct: 131 ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 190

Query: 244 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 303
           YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS
Sbjct: 191 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 250

Query: 304 CAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQI-- 363
           CAAGNDGPELSTV NVAPWIMTVAATGIDRRF TAFKLGNGKKFTGFSINTFSP+NQ+  
Sbjct: 251 CAAGNDGPELSTVVNVAPWIMTVAATGIDRRFVTAFKLGNGKKFTGFSINTFSPRNQMYS 310

Query: 364 ------------------ASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGIIELL 423
                             ASACD DALSQSKVKGRIVYCLTT  DYNIESLGGTGIIELL
Sbjct: 311 LTSGAKASNNGTTLRNASASACDADALSQSKVKGRIVYCLTTFTDYNIESLGGTGIIELL 370

Query: 424 EEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLSRGP 483
           E+QTDSSPILLLPGAVIPPVSGKYIDLYIN+TKDPRAIIYKSKTVKIAAPFVASF SRGP
Sbjct: 371 EKQTDSSPILLLPGAVIPPVSGKYIDLYINATKDPRAIIYKSKTVKIAAPFVASFSSRGP 430

Query: 484 QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY 543
           QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY
Sbjct: 431 QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY 490

Query: 544 VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYI 603
           VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQ+NP KAVHPGLVYDISL SYI
Sbjct: 491 VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQVNPAKAVHPGLVYDISLDSYI 550

Query: 604 SFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTV 663
           SFLCKEGYNSTTIGLLVGSKEYNCSKIKPA+GTDGLNYPTMHKQLSDPGSAITAVFYRTV
Sbjct: 551 SFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITAVFYRTV 610

Query: 664 TDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALL 721
           T VE+GASVYRANISSPVGLSVKVFPDSLDFAKAGERK FKVVVKGEAMRDGTRILSA L
Sbjct: 611 THVEYGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTRILSASL 670

BLAST of Cp4.1LG19g04950 vs. NCBI nr
Match: XP_023518863.1 (subtilisin-like protease SBT4.15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1232 bits (3188), Expect = 0.0
Identity = 628/649 (96.76%), Postives = 629/649 (96.92%), Query Frame = 0

Query: 97  MEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG 156
           MEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG
Sbjct: 1   MEGVVSVFPSRKHRVVTTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEG 60

Query: 157 YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAG 216
           YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAG
Sbjct: 61  YGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAG 120

Query: 217 SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI 276
           SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI
Sbjct: 121 SAVEGASLYGLGKGTARGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSI 180

Query: 277 GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFA 336
           GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFA
Sbjct: 181 GSVAVDFFRDSHAIGAFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFA 240

Query: 337 TAFKLGNGKKFTGFSINTFSPKNQI--------------------ASACDVDALSQSKVK 396
           TAFKLGNGKKFTGFSINTFSPKNQ+                    ASACDVDALSQSKVK
Sbjct: 241 TAFKLGNGKKFTGFSINTFSPKNQMYSLTSGARASNNGTILGNASASACDVDALSQSKVK 300

Query: 397 GRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTK 456
           GRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTK
Sbjct: 301 GRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTK 360

Query: 457 DPRAIIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI 516
           DPRAIIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI
Sbjct: 361 DPRAIIYKSKTVKIAAPFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI 420

Query: 517 ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF 576
           ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF
Sbjct: 421 ADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEF 480

Query: 577 GSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGT 636
           GSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGT
Sbjct: 481 GSGAGQINPTKAVHPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGT 540

Query: 637 DGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAK 696
           DGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAK
Sbjct: 541 DGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAK 600

Query: 697 AGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILISRQSRLT 725
           AGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILISRQSRLT
Sbjct: 601 AGERKRFKVVVKGEAMRDGTRILSALLEWKDSKHVVRSNILISRQSRLT 649

BLAST of Cp4.1LG19g04950 vs. ExPASy TrEMBL
Match: A0A6J1HD77 (subtilisin-like protease SBT4.15 OS=Cucurbita moschata OX=3662 GN=LOC111463066 PE=3 SV=1)

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 643/684 (94.01%), Postives = 648/684 (94.74%), Query Frame = 0

Query: 58  LDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSW 117
           LD+   ENKARESKIHSYGRSFNGFAARLLPHEAAKLSN+EGVVSVFPSRKHRVVTTRSW
Sbjct: 8   LDSYVSENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSW 67

Query: 118 DFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC 177
           +FLGLNRRSKRN KIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC
Sbjct: 68  NFLGLNRRSKRNLKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTAC 127

Query: 178 NNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP 237
           NNKVIGANYFDLDNVSQFTE SVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP
Sbjct: 128 NNKVIGANYFDLDNVSQFTEPSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVP 187

Query: 238 SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK 297
           SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK
Sbjct: 188 SARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMK 247

Query: 298 KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSP 357
           KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRF TAFKLGN KKFTGFSINTFSP
Sbjct: 248 KGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFVTAFKLGNDKKFTGFSINTFSP 307

Query: 358 KNQ--------------------IASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGT 417
           +NQ                     ASACDVDALSQSKVKGRIVYCLTT  DYNIESLGGT
Sbjct: 308 RNQKYSLTSGAKASNNGTILGNASASACDVDALSQSKVKGRIVYCLTTFTDYNIESLGGT 367

Query: 418 GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS 477
           GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS
Sbjct: 368 GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVAS 427

Query: 478 FLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA 537
           F SRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA
Sbjct: 428 FSSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHA 487

Query: 538 TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI 597
           TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI
Sbjct: 488 TAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDI 547

Query: 598 SLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITA 657
           SL SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPA+GTDGLNYPTMHKQLSDPGSAITA
Sbjct: 548 SLNSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITA 607

Query: 658 VFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTR 717
           VFYRTVT VEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERK FKVVVKGEAMRDGTR
Sbjct: 608 VFYRTVTHVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTR 667

Query: 718 ILSALLEWKDSKHVVRSNILISRQ 721
           ILSALLEWK SKHVVRSNILI RQ
Sbjct: 668 ILSALLEWKGSKHVVRSNILIYRQ 691

BLAST of Cp4.1LG19g04950 vs. ExPASy TrEMBL
Match: A0A6J1I5A0 (subtilisin-like protease SBT4.15 OS=Cucurbita maxima OX=3661 GN=LOC111471144 PE=3 SV=1)

HSP 1 Score: 1252 bits (3240), Expect = 0.0
Identity = 637/678 (93.95%), Postives = 645/678 (95.13%), Query Frame = 0

Query: 64  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGLN 123
           ENKARESKIHSYGRSFNGFAARLLPHEAAKLSN+EGVVSVFPSRKHRVVTTRSWDFLGLN
Sbjct: 11  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNVEGVVSVFPSRKHRVVTTRSWDFLGLN 70

Query: 124 RRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 183
            RSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG
Sbjct: 71  HRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 130

Query: 184 ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 243
           ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV
Sbjct: 131 ANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 190

Query: 244 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 303
           YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS
Sbjct: 191 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 250

Query: 304 CAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQI-- 363
           CAAGNDGPELSTV NVAPWIMTVAATGIDRRF TAFKLGNGKKFTGFSINTFSP+NQ+  
Sbjct: 251 CAAGNDGPELSTVVNVAPWIMTVAATGIDRRFVTAFKLGNGKKFTGFSINTFSPRNQMYS 310

Query: 364 ------------------ASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGIIELL 423
                             ASACD DALSQSKVKGRIVYCLTT  DYNIESLGGTGIIELL
Sbjct: 311 LTSGAKASNNGTTLRNASASACDADALSQSKVKGRIVYCLTTFTDYNIESLGGTGIIELL 370

Query: 424 EEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLSRGP 483
           E+QTDSSPILLLPGAVIPPVSGKYIDLYIN+TKDPRAIIYKSKTVKIAAPFVASF SRGP
Sbjct: 371 EKQTDSSPILLLPGAVIPPVSGKYIDLYINATKDPRAIIYKSKTVKIAAPFVASFSSRGP 430

Query: 484 QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY 543
           QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY
Sbjct: 431 QLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAY 490

Query: 544 VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYI 603
           VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQ+NP KAVHPGLVYDISL SYI
Sbjct: 491 VKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQVNPAKAVHPGLVYDISLDSYI 550

Query: 604 SFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTV 663
           SFLCKEGYNSTTIGLLVGSKEYNCSKIKPA+GTDGLNYPTMHKQLSDPGSAITAVFYRTV
Sbjct: 551 SFLCKEGYNSTTIGLLVGSKEYNCSKIKPAQGTDGLNYPTMHKQLSDPGSAITAVFYRTV 610

Query: 664 TDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALL 721
           T VE+GASVYRANISSPVGLSVKVFPDSLDFAKAGERK FKVVVKGEAMRDGTRILSA L
Sbjct: 611 THVEYGASVYRANISSPVGLSVKVFPDSLDFAKAGERKTFKVVVKGEAMRDGTRILSASL 670

BLAST of Cp4.1LG19g04950 vs. ExPASy TrEMBL
Match: A0A6J1CRC3 (subtilisin-like protease SBT4.15 OS=Momordica charantia OX=3673 GN=LOC111013614 PE=3 SV=1)

HSP 1 Score: 1135 bits (2937), Expect = 0.0
Identity = 574/741 (77.46%), Postives = 636/741 (85.83%), Query Frame = 0

Query: 1   MRLHELLFVLVLLFWLVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDT 60
           M+LHE L + VL+ WLVAS MI GS H+ERKPYIVYMG++P G+ ST V D HH+LLLD 
Sbjct: 1   MKLHENLLIFVLVIWLVASLMIQGSDHHERKPYIVYMGELPLGSRST-VADHHHSLLLDA 60

Query: 61  IGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFL 120
           IGDE  ARESKIHSYG+SFNGFAARLLPHEA K+S  E VVSVF SRK R+VTTRSWDFL
Sbjct: 61  IGDEKTARESKIHSYGKSFNGFAARLLPHEADKISKREDVVSVFRSRKQRIVTTRSWDFL 120

Query: 121 GLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNK 180
           GLN+RSKRNP +E+N+IVAVLDTGIWINSPSFSD+GYGPPPAKWKG+CVTG NF+ACNNK
Sbjct: 121 GLNKRSKRNPSLEANLIVAVLDTGIWINSPSFSDKGYGPPPAKWKGKCVTGANFSACNNK 180

Query: 181 VIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSAR 240
           VIGA YFDLD+ +     SVADTEGHGSHTASTVAGSAVEGASLYGL +GTARGGVPSAR
Sbjct: 181 VIGAAYFDLDDQNPAAARSVADTEGHGSHTASTVAGSAVEGASLYGLARGTARGGVPSAR 240

Query: 241 IAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGI 300
           IAVYKVCWSIFCS+MDVLAGFD+AIADGVD ISVSIGS  +D FRDS AIGAFHAMKKGI
Sbjct: 241 IAVYKVCWSIFCSEMDVLAGFDQAIADGVDLISVSIGSPPMDLFRDSQAIGAFHAMKKGI 300

Query: 301 LTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQ 360
           LTS AAGN GP+LSTVENVAPWIMTVAAT IDRRF T  +LGNG KFTGFSINTFS   Q
Sbjct: 301 LTSSAAGNYGPQLSTVENVAPWIMTVAATAIDRRFITVLQLGNGSKFTGFSINTFSGGKQ 360

Query: 361 I--------------------ASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGII 420
           +                    ASACD +ALSQSKVKGRIVYCL    D NI+SLGG G+I
Sbjct: 361 MQPLTSGAKAANATAGSNRGNASACDAEALSQSKVKGRIVYCLGNYADPNIKSLGGVGVI 420

Query: 421 ELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLS 480
           +LL+ QTD S IL+LPGA IP +SGK +DLYINSTK+PRA+IYKS+T+KI APFVASF S
Sbjct: 421 QLLQPQTDYSSILVLPGAAIPSISGKSVDLYINSTKNPRAVIYKSRTIKITAPFVASFSS 480

Query: 481 RGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAA 540
           RGPQ I+ NILKPDLAAPGIDILAAYT+L+SLTG  +DSRYSLF VMAGTSMACPHATAA
Sbjct: 481 RGPQSITRNILKPDLAAPGIDILAAYTELASLTGDTSDSRYSLFKVMAGTSMACPHATAA 540

Query: 541 AAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISLG 600
           AAYVKSFHPDWSPAAVKSALMTTATPMKIK ++AE GSGAGQINPT+AVHPGLVYDISL 
Sbjct: 541 AAYVKSFHPDWSPAAVKSALMTTATPMKIKFKEAELGSGAGQINPTRAVHPGLVYDISLN 600

Query: 601 SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFY 660
           SY+SFLCK+GYNST IGL+VGSK+YNCS +KPA+GTDGLNYPTMH+QLSDP SAI AVFY
Sbjct: 601 SYVSFLCKQGYNSTAIGLIVGSKKYNCSMLKPARGTDGLNYPTMHQQLSDPSSAIDAVFY 660

Query: 661 RTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILS 720
           RTVT+V +GAS+YRANI+SP GLSVKVFPD+L+FAK+ ERK FKVVVKG  M  G +ILS
Sbjct: 661 RTVTNVGYGASLYRANITSPDGLSVKVFPDTLNFAKSHERKTFKVVVKGNPMPKGIQILS 720

BLAST of Cp4.1LG19g04950 vs. ExPASy TrEMBL
Match: A0A0A0KFB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G451510 PE=3 SV=1)

HSP 1 Score: 1134 bits (2932), Expect = 0.0
Identity = 579/742 (78.03%), Postives = 638/742 (85.98%), Query Frame = 0

Query: 1   MRLHELLFVLVLLFWLVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDT 60
           M+LHE LFV VLL W +ASFMIHGS+H+ERKPYIVYMGD+PAG+ STTV DDHHNLLLD 
Sbjct: 1   MKLHEGLFVFVLLLWFIASFMIHGSNHHERKPYIVYMGDLPAGSPSTTVADDHHNLLLDA 60

Query: 61  IGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFL 120
           IGDE  ARESKI+SYG+SFNGFAARLLP EA KLS+ E VVSVF SRK RV+TTRSW+FL
Sbjct: 61  IGDEKIARESKIYSYGKSFNGFAARLLPDEATKLSDEESVVSVFESRKKRVLTTRSWEFL 120

Query: 121 GLNRR-SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNN 180
           GLN + SKRNP IESN+IVAV DT    +SPSFSDEGYGPPP KWKG+CVTGPNFTACNN
Sbjct: 121 GLNHQYSKRNPLIESNLIVAVFDT----DSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 180

Query: 181 KVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSA 240
           KVIGANYFDLD V+ + E SVADT+GHGSH ASTVAGSAV GASLYGL KGTARGGVPSA
Sbjct: 181 KVIGANYFDLDKVTSYPELSVADTDGHGSHIASTVAGSAVAGASLYGLAKGTARGGVPSA 240

Query: 241 RIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKG 300
           RIAVYKVCWS+FC++MDVLA FDEAIADGVD ISVSIGS  +DFFRD  AIGAFHAMKKG
Sbjct: 241 RIAVYKVCWSVFCNEMDVLAAFDEAIADGVDLISVSIGSPPMDFFRDGQAIGAFHAMKKG 300

Query: 301 ILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKN 360
           ILT+ AAGNDGPEL TVENVAPWIMTVAATGIDR F TAF+LGNG KFTG SINTFSP+ 
Sbjct: 301 ILTTTAAGNDGPELFTVENVAPWIMTVAATGIDRGFVTAFELGNGNKFTGGSINTFSPQK 360

Query: 361 QI--------------------ASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGI 420
           Q+                    ASACD +A++QSKVKG+IVYCL T  D +I+SLGGTG+
Sbjct: 361 QMHSLTSGAKAAFNNGTPHQGNASACDPNAMNQSKVKGKIVYCLKTYTDPSIKSLGGTGV 420

Query: 421 IELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFL 480
           I+L ++QTD S ILLLPGA IP VSGKYIDLYINSTK+P+A+IYKS+TVKI APFVASF 
Sbjct: 421 IQLTQQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 480

Query: 481 SRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATA 540
           SRGPQ IS NILKPDL+APGIDILAAYTKL++LTG  +DSRYS F+VM+GTSMAC HATA
Sbjct: 481 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACSHATA 540

Query: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISL 600
           AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSED   GSGAGQINPTKAVHPGLVY+IS 
Sbjct: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDVVLGSGAGQINPTKAVHPGLVYNISF 600

Query: 601 GSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVF 660
            SYISFLCKEGYN+TTIGLL GSK+YNCSKIKPA+GTDGLNYPTMHKQLSDP SAI AVF
Sbjct: 601 DSYISFLCKEGYNNTTIGLLGGSKKYNCSKIKPAQGTDGLNYPTMHKQLSDPSSAIEAVF 660

Query: 661 YRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRIL 720
           YRTVT V +GAS+YRANISSP  LSVKVFPD+L+F K  E + FKVVVKG+ M  GT+IL
Sbjct: 661 YRTVTHVGYGASLYRANISSPDSLSVKVFPDTLNFVKLHETRTFKVVVKGKPMPKGTQIL 720

BLAST of Cp4.1LG19g04950 vs. ExPASy TrEMBL
Match: A0A1S4E3S0 (subtilisin-like protease SBT4.15 OS=Cucumis melo OX=3656 GN=LOC103500883 PE=3 SV=1)

HSP 1 Score: 1056 bits (2731), Expect = 0.0
Identity = 538/682 (78.89%), Postives = 588/682 (86.22%), Query Frame = 0

Query: 61  IGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFL 120
           +G E  ARESKI+SYG+SFNGFAARLLPHEA K+SN E VVSVF SRK RVVTTRSWDFL
Sbjct: 1   LGSEKIARESKIYSYGKSFNGFAARLLPHEATKISNEESVVSVFESRKKRVVTTRSWDFL 60

Query: 121 GLNRR-SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNN 180
           GLN R SKRNP IESN+I+AV DTGIWI+SPSFSDEGYGPPP KWKG+CVTGPNFTACNN
Sbjct: 61  GLNHRYSKRNPLIESNLIIAVFDTGIWIDSPSFSDEGYGPPPPKWKGKCVTGPNFTACNN 120

Query: 181 KVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSA 240
           KVIGANYFDL  VS + E SVADTEGHGSHTASTVAGSAV GASLYGL KGTARGGVPSA
Sbjct: 121 KVIGANYFDLAKVSSYPELSVADTEGHGSHTASTVAGSAVAGASLYGLAKGTARGGVPSA 180

Query: 241 RIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKG 300
           RIAVYKVCWSIFC++MDVLA FDEAIADGVD ISVSIGS A+DFFRD  AIGAFHAMKKG
Sbjct: 181 RIAVYKVCWSIFCNEMDVLAAFDEAIADGVDLISVSIGSPAMDFFRDGQAIGAFHAMKKG 240

Query: 301 ILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKN 360
           ILT+ AAGNDGP+LSTVENVAPWIMTVAAT IDR F T+F+LGNG K TG SINTFSP+ 
Sbjct: 241 ILTTAAAGNDGPQLSTVENVAPWIMTVAATAIDRGFVTSFELGNGNKLTGGSINTFSPQK 300

Query: 361 QI--------------------ASACDVDALSQSKVKGRIVYCLTTSIDYNIESLGGTGI 420
           Q+                    ASACD +A++QSKVKG+IVYCL T  D +I+SLGGTG+
Sbjct: 301 QMYSLTSGAKAASNNATPHQGNASACDPNAINQSKVKGKIVYCLKTYTDPSIKSLGGTGV 360

Query: 421 IELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFL 480
           I+L  +QTD S ILLLPGA IP VSGKYIDLYINSTK+P+A+IYKS+TVKI APFVASF 
Sbjct: 361 IQLTTQQTDYSSILLLPGATIPSVSGKYIDLYINSTKNPKAVIYKSETVKIDAPFVASFS 420

Query: 481 SRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATA 540
           SRGPQ IS NILKPDL+APGIDILAAYTKL++LTG  +DSRYS F+VM+GTSMACPHATA
Sbjct: 421 SRGPQRISSNILKPDLSAPGIDILAAYTKLATLTGDTSDSRYSFFTVMSGTSMACPHATA 480

Query: 541 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVYDISL 600
           AAAYVKSFHPDWSPAAVKSALMTTATPMKIKS+DA  GSGAGQINPTKAVHPGLVY+IS 
Sbjct: 481 AAAYVKSFHPDWSPAAVKSALMTTATPMKIKSKDAMLGSGAGQINPTKAVHPGLVYNISF 540

Query: 601 GSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVF 660
            SYISFLCKEGYNSTTIGLL GSK+YNC+KIKPA+GTDGLNYPTMHKQLS P S I AVF
Sbjct: 541 NSYISFLCKEGYNSTTIGLLGGSKKYNCTKIKPAQGTDGLNYPTMHKQLSGPSSTIEAVF 600

Query: 661 YRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRIL 720
           YRTVT V +GAS+YRANISSP  LSVKVFPD+L+F K  E K FKVVVKG+ M  GT+IL
Sbjct: 601 YRTVTHVGYGASLYRANISSPASLSVKVFPDTLNFVKLHETKTFKVVVKGKPMPKGTQIL 660

BLAST of Cp4.1LG19g04950 vs. TAIR 10
Match: AT5G03620.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 793.9 bits (2049), Expect = 1.1e-229
Identity = 413/753 (54.85%), Postives = 533/753 (70.78%), Query Frame = 0

Query: 9   VLVLLFWLVASFMIHGS--SHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENK 68
           + +L F LV + +I  +   + ERKPYIVYMG+    +L     ++HHNLL+  IGDE+K
Sbjct: 9   LFMLCFCLVNNAVIAATEDENVERKPYIVYMGEATENSL-VEAAENHHNLLMTVIGDESK 68

Query: 69  ARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGL-NRR 128
           ARE KI+SYG++ NGF ARL PHEA KLS  EGVVSVF + + ++ TTRSWDFLGL   +
Sbjct: 69  ARELKIYSYGKNINGFVARLFPHEAEKLSREEGVVSVFKNTQRQLHTTRSWDFLGLVESK 128

Query: 129 SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIGAN 188
            KR+  IESNIIV VLDTGI + SPSF+D+G GPPPAKWKG+CVTG NFT CNNKVIGA 
Sbjct: 129 YKRSVGIESNIIVGVLDTGIDVESPSFNDKGVGPPPAKWKGKCVTGNNFTRCNNKVIGAK 188

Query: 189 YFDL--DNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAV 248
           YF +  + +      + AD +GHG+HT+ST+AG +V  ASL+G+  GTARGGVPSARIA 
Sbjct: 189 YFHIQSEGLPDGEGDTAADHDGHGTHTSSTIAGVSVSSASLFGIANGTARGGVPSARIAA 248

Query: 249 YKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGILTS 308
           YKVCW   C+DMD+LA FDEAI+DGVD IS+SIG  ++ FF D  AIGAFHAMK+GILT+
Sbjct: 249 YKVCWDSGCTDMDMLAAFDEAISDGVDIISISIGGASLPFFEDPIAIGAFHAMKRGILTT 308

Query: 309 CAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQI-- 368
           C+AGN+GP L TV N+APW+MTVAA  +DR+F T  KLGNG   +G S+N F+P+ ++  
Sbjct: 309 CSAGNNGPGLFTVSNLAPWVMTVAANSLDRKFETVVKLGNGLTASGISLNGFNPRKKMYP 368

Query: 369 -----------------ASACDVDALSQSKVKGRIVYCLT---------TSIDYNIESLG 428
                             S C+   L + KV G++VYC              D+ + SL 
Sbjct: 369 LTSGSLASNLSAGGYGEPSTCEPGTLGEDKVMGKVVYCEAGREEGGNGGQGQDHVVRSLK 428

Query: 429 GTGIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFV 488
           G G+I  L E TD +   L+ G+ +    G  I  YINSTK+P+A+I+K+KT K+ AP +
Sbjct: 429 GAGVIVQLLEPTDMATSTLIAGSYVFFEDGTKITEYINSTKNPQAVIFKTKTTKMLAPSI 488

Query: 489 ASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACP 548
           +SF +RGPQ ISPNILKPD++APG++ILAAY+KL+S+TG   D+R +LFS+M+GTSMACP
Sbjct: 489 SSFSARGPQRISPNILKPDISAPGLNILAAYSKLASVTGYPDDNRRTLFSIMSGTSMACP 548

Query: 549 HATAAAAYVKSFHPDWSPAAVKSALMTTATPMKIKSEDAEFGSGAGQINPTKAVHPGLVY 608
           HA AAAAYVKSFHPDWSPAA+KSALMTTATPM+IK  +AE   G+GQINP +A+HPGLVY
Sbjct: 549 HAAAAAAYVKSFHPDWSPAAIKSALMTTATPMRIKGNEAELSYGSGQINPRRAIHPGLVY 608

Query: 609 DISLGSYISFLCKEGYNSTTIGLLVG-------SKEYNCSKIKPAKGTDGLNYPTMHKQL 668
           DI+  +Y+ FLCKEGYNST+IGLL G        KEYNC  IK   G+DGLNYP++HKQ+
Sbjct: 609 DITEDAYLRFLCKEGYNSTSIGLLTGDNSNNTTKKEYNCENIKRGLGSDGLNYPSLHKQV 668

Query: 669 SDPGSAITAVFYRTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVK 721
           +   + ++ VFYRTVT+V +G S Y A + +P GL V+V P  + F +  E++ FKVV+ 
Sbjct: 669 NSTEAKVSEVFYRTVTNVGYGPSTYVARVWAPKGLRVEVVPKVMSFERPKEKRNFKVVID 728

BLAST of Cp4.1LG19g04950 vs. TAIR 10
Match: AT4G00230.1 (xylem serine peptidase 1 )

HSP 1 Score: 674.1 bits (1738), Expect = 1.3e-193
Identity = 361/738 (48.92%), Postives = 482/738 (65.31%), Query Frame = 0

Query: 4   HELLFVLVLLFWLVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGD 63
           H LL +++++ W+   +      H  +  YI+Y+GD P  T  T  +  H NLL      
Sbjct: 10  HHLLVLVMVVLWISPRYASAEDEH-AKDFYIIYLGDRPDNTEET--IKTHINLLSSLNIS 69

Query: 64  ENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGLN 123
           + +A+E K++SY ++FN FAA+L PHEA K+  ME VVSV  ++  ++ TT+SWDF+GL 
Sbjct: 70  QEEAKERKVYSYTKAFNAFAAKLSPHEAKKMMEMEEVVSVSRNQYRKLHTTKSWDFVGLP 129

Query: 124 RRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIG 183
             +KR+ K E ++I+ VLDTGI  +S SF D G GPPPAKWKG C    NFT CNNK+IG
Sbjct: 130 LTAKRHLKAERDVIIGVLDTGITPDSESFLDHGLGPPPAKWKGSCGPYKNFTGCNNKIIG 189

Query: 184 ANYFDLD-NVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIA 243
           A YF  D NV      S  D +GHG+HT+STVAG  V  ASLYG+  GTARG VPSAR+A
Sbjct: 190 AKYFKHDGNVPAGEVRSPIDIDGHGTHTSSTVAGVLVANASLYGIANGTARGAVPSARLA 249

Query: 244 VYKVCWS-IFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFRDSHAIGAFHAMKKGIL 303
           +YKVCW+   C+DMD+LAGF+ AI DGV+ IS+SIG    D+  DS ++G+FHAM+KGIL
Sbjct: 250 MYKVCWARSGCADMDILAGFEAAIHDGVEIISISIGGPIADYSSDSISVGSFHAMRKGIL 309

Query: 304 TSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPK--- 363
           T  +AGNDGP   TV N  PWI+TVAA+GIDR F +   LGNGK F+G  I+ FSPK   
Sbjct: 310 TVASAGNDGPSSGTVTNHEPWILTVAASGIDRTFKSKIDLGNGKSFSGMGISMFSPKAKS 369

Query: 364 ---------------NQIASACDVDALSQSKVKGRIVYCLT--TSIDYNIESLGGTGIIE 423
                            +A  C  D+L + KVKG+++ C      ++  I+S GG G I 
Sbjct: 370 YPLVSGVDAAKNTDDKYLARYCFSDSLDRKKVKGKVMVCRMGGGGVESTIKSYGGAGAII 429

Query: 424 LLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTVKIAAPFVASFLSR 483
           + ++  D++ I + P   +    G  I  YINST+   A+I K++ V I APFVASF SR
Sbjct: 430 VSDQYLDNAQIFMAPATSVNSSVGDIIYRYINSTRSASAVIQKTRQVTIPAPFVASFSSR 489

Query: 484 GPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAA 543
           GP   S  +LKPD+AAPGIDILAA+T   SLTG   D+++S F++++GTSMACPH    A
Sbjct: 490 GPNPGSIRLLKPDIAAPGIDILAAFTLKRSLTGLDGDTQFSKFTILSGTSMACPHVAGVA 549

Query: 544 AYVKSFHPDWSPAAVKSALMTTATPMKIK-SEDAEFGSGAGQINPTKAVHPGLVYDISLG 603
           AYVKSFHPDW+PAA+KSA++T+A P+  + ++DAEF  G GQINP +A  PGLVYD+   
Sbjct: 550 AYVKSFHPDWTPAAIKSAIITSAKPISRRVNKDAEFAYGGGQINPRRAASPGLVYDMDDI 609

Query: 604 SYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFY 663
           SY+ FLC EGYN+TT+  LVG++  +CS I P  G D LNYPT+   L    ++  AVF 
Sbjct: 610 SYVQFLCGEGYNATTLAPLVGTRSVSCSSIVPGLGHDSLNYPTIQLTLRSAKTSTLAVFR 669

Query: 664 RTVTDVEHGASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILS 719
           R VT+V   +SVY A + +P G+ + V P SL F+KA +++ FKVVVK + M  G +I+S
Sbjct: 670 RRVTNVGPPSSVYTATVRAPKGVEITVEPQSLSFSKASQKRSFKVVVKAKQMTPG-KIVS 729

BLAST of Cp4.1LG19g04950 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 548.5 bits (1412), Expect = 8.0e-156
Identity = 318/691 (46.02%), Postives = 430/691 (62.23%), Query Frame = 0

Query: 53  HHNLLLDTIGDENKARESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVV 112
           HH  +L  +     A    + SY RSFNGFAA L   E+ KL NM+ VVSVFPS+ H + 
Sbjct: 14  HHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKEVVSVFPSKSHELT 73

Query: 113 TTRSWDFLGLNRRSKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGP 172
           TTRSWDF+G   +++R    ES++IV V+D+GIW  S SF DEG+GPPP KWKG C  G 
Sbjct: 74  TTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGPPPKKWKGSCKGGL 133

Query: 173 NFTACNNKVIGANYFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTA 232
            F ACNNK+IGA ++     ++F + S  D EGHG+HTAST AG+AV+ AS YGL +GTA
Sbjct: 134 KF-ACNNKLIGARFY-----NKFAD-SARDEEGHGTHTASTAAGNAVQAASFYGLAQGTA 193

Query: 233 RGGVPSARIAVYKVCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAV-DFFRDSHAIG 292
           RGGVPSARIA YKVC++  C+D+D+LA FD+AIADGVD IS+SI +  V +    S AIG
Sbjct: 194 RGGVPSARIAAYKVCFN-RCNDVDILAAFDDAIADGVDVISISISADYVSNLLNASVAIG 253

Query: 293 AFHAMKKGILTSCAAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFS 352
           +FHAM +GI+T+ +AGN+GP+  +V NV+PW++TVAA+G DR+F     LGNGK  TG S
Sbjct: 254 SFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGKALTGIS 313

Query: 353 INTF-----------------SPKNQIASACDVDALSQSKVKGRIVYCLTTSIDYNIESL 412
           +NTF                 +     A  C    +    VKG+IV C    + Y    L
Sbjct: 314 VNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLC-DDFLGYREAYL 373

Query: 413 GGT-GIIELLEEQTDSSPILLLPGAVIPPVSGKYIDLYINSTKDPRA-IIYKSKTVKIAA 472
            G  G+I       DS+ ++  P + +     K I  YI S + P+A I+   + V   A
Sbjct: 374 AGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEIVDREA 433

Query: 473 PFVASFLSRGPQLISPNILKPDLAAPGIDILAAYTKLSSLTGKI--ADSRYSLFSVMAGT 532
           P+V SF SRGP  +  N+LKPD++APG++ILAA++ ++S +  +   D R   +SVM+GT
Sbjct: 434 PYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYSVMSGT 493

Query: 533 SMACPHATAAAAYVKSFHPDWSPAAVKSALMTTATPMKI-KSEDAEFGSGAGQINPTKAV 592
           SMACPH    AAYVKSFHPDWSP+A+KSA+MTTATPM + K+ + EF  G+GQINPTKA 
Sbjct: 494 SMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEFAYGSGQINPTKAS 553

Query: 593 HPGLVYDISLGSYISFLCKEGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLS 652
            PGLVY++    Y+  LC EG++STT+    G +   CS+    K    LNYPTM   +S
Sbjct: 554 DPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG-QNVTCSERTEVK---DLNYPTMTTFVS 613

Query: 653 --DPGSAITAVFYRTVTDVEHGASVYRAN-ISSPVGLSVKVFPDSLDFAKAGERKRFKVV 712
             DP       F RTVT+V    S Y+A+ +     L + + P+ L F    E+K F V 
Sbjct: 614 SLDP---FNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLEEKKSFVVT 673

Query: 713 VKGEAMRDGTRILSALLEWKDSKHVVRSNIL 718
           + G+ ++DG+ + S+++ W D  H VRS I+
Sbjct: 674 ISGKELKDGSFVSSSVV-WSDGSHSVRSPIV 687

BLAST of Cp4.1LG19g04950 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 1.2e-151
Identity = 317/730 (43.42%), Postives = 436/730 (59.73%), Query Frame = 0

Query: 16  LVASFMIHGSSHYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENKARESKIHSY 75
           LV SF   G    +++ YIVYMG +P+      +   HH  +L  +  E+  ++  + +Y
Sbjct: 17  LVVSFASAGKDDQDKQVYIVYMGALPSRV--DYMPMSHHTSILQDVTGESSIQDRLVRNY 76

Query: 76  GRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGL--NRRSKRNPKIE 135
            RSFNGFAARL   E   L++M+ VVSVFPS+   + TT SW+F+GL   +R+KRNP IE
Sbjct: 77  KRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMGLKEGKRTKRNPLIE 136

Query: 136 SNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIGANYFDLDNVS 195
           S+ I+ V+D+GI+  S SFS +G+GPPP KWKG C  G NFT CNNK+IGA Y+    + 
Sbjct: 137 SDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFT-CNNKLIGARYY-TPKLE 196

Query: 196 QFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYKVC--WSIF 255
            F E S  D  GHGSHTAS  AG+AV+  S YGLG GT RGGVP+ARIAVYKVC    I 
Sbjct: 197 GFPE-SARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAARIAVYKVCDPGVIR 256

Query: 256 CSDMDVLAGFDEAIADGVDFISVSIGSVAVDFF-RDSHAIGAFHAMKKGILTSCAAGNDG 315
           C+   +LA FD+AIAD VD I+VS+G+ AV  F  D+ AIGAFHAM KGILT   AGN+G
Sbjct: 257 CTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHAMAKGILTVNGAGNNG 316

Query: 316 PELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQ---------I 375
           PE  T+ ++APW+ TVAA+ ++R F T   LGNGK   G S+N+F    +          
Sbjct: 317 PERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSFDLNGKKYPLVYGKSA 376

Query: 376 ASACDVDA--------LSQSKVKGRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPIL 435
           +S CD  +        L   +VKG+IV C T       +++G    I +     D++ + 
Sbjct: 377 SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAVASI-VRNPYEDAASVF 436

Query: 436 LLPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTV-KIAAPFVASFLSRGPQLISPNILK 495
             P +V+       +  Y+NSTK+P+A + KS+T+    AP VAS+ SRGP  +  +ILK
Sbjct: 437 SFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVASYSSRGPNPLIHDILK 496

Query: 496 PDLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWS 555
           PD+ APG +ILAAY+    +    +D+R+  ++V++GTSM+CPH    AAY+K+FHP WS
Sbjct: 497 PDITAPGSEILAAYSPY--VPPSESDTRHVKYTVISGTSMSCPHVAGVAAYIKTFHPLWS 556

Query: 556 PAAVKSALMTTATPMKI----KSEDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCK 615
           P+ ++SA+MTTA PM       +E AEF  GAG ++P  A+HPGLVY+ +   +I+FLC 
Sbjct: 557 PSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCG 616

Query: 616 EGYNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTVTDVEH 675
             Y    + L+ G    +C+K +    T  LNYP+M  Q+S         F RTVT+V  
Sbjct: 617 FNYTGKKLRLISGDSS-SCTKEQTKSLTRNLNYPSMSAQVSGT-KPFKVTFRRTVTNVGR 676

Query: 676 GASVYRANISSPVGLSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDS 719
             + Y+A +     L VKV P  L      E+K F V V G   +    ++SA L W D 
Sbjct: 677 PNATYKAKVVGS-KLKVKVVPAVLSLKSLYEKKSFTVTVSGAGPK-AENLVSAQLIWSDG 734

BLAST of Cp4.1LG19g04950 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 531.6 bits (1368), Expect = 1.0e-150
Identity = 313/730 (42.88%), Postives = 425/730 (58.22%), Query Frame = 0

Query: 12  LLFWLVASFMIHGSS----HYERKPYIVYMGDMPAGTLSTTVVDDHHNLLLDTIGDENKA 71
           L+  ++ SF+I  S+      +++ Y+VYMG +P+  L  T +  HH  +L  +  E+  
Sbjct: 10  LISCVLVSFVISVSAVTDDSQDKQVYVVYMGSLPSSRLEYTPM-SHHMSILQEVTGESSV 69

Query: 72  RESKIHSYGRSFNGFAARLLPHEAAKLSNMEGVVSVFPSRKHRVVTTRSWDFLGL--NRR 131
               + SY RSFNGFAARL   E  +++ MEGVVSVFP   +++ TT SWDFLGL   + 
Sbjct: 70  EGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGKN 129

Query: 132 SKRNPKIESNIIVAVLDTGIWINSPSFSDEGYGPPPAKWKGRCVTGPNFTACNNKVIGAN 191
           +KRN  IES+ I+  +D+GIW  S SFSD+G+GPPP KWKG C  G NFT CNNK+IGA 
Sbjct: 130 TKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFT-CNNKLIGAR 189

Query: 192 YFDLDNVSQFTETSVADTEGHGSHTASTVAGSAVEGASLYGLGKGTARGGVPSARIAVYK 251
                    +T     D EGHG+HTAST AG+AV+  S YG+G GTARGGVP++RIA YK
Sbjct: 190 --------DYTNEGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAYK 249

Query: 252 VCWSIFCSDMDVLAGFDEAIADGVDFISVSIGSVAVDFFR-DSHAIGAFHAMKKGILTSC 311
            C  + C+   VL+ FD+AIADGVD IS+S+G+  V  +  D  AIGAFHAM KGILT  
Sbjct: 250 ACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTVQ 309

Query: 312 AAGNDGPELSTVENVAPWIMTVAATGIDRRFATAFKLGNGKKFTGFSINTFSPKNQ---- 371
           +AGN GP   +V +VAPWI+TVAA+  +R F T   LGNGK F G S+N F  K +    
Sbjct: 310 SAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYPL 369

Query: 372 IASACDVDAL------SQSKVKGRIVYCLTTSIDYNIESLGGTGIIELLEEQTDSSPILL 431
              + D   L      S+ KV   IV                  +  + E   D + + +
Sbjct: 370 YGGSTDGPLLRGKILVSEDKVSSEIV------------------VANINENYHDYAYVSI 429

Query: 432 LPGAVIPPVSGKYIDLYINSTKDPRAIIYKSKTV-KIAAPFVASFLSRGPQLISPNILKP 491
           LP + +       +  Y+NSTK P   + KS+ +   AAP VA F SRGP  I+ +ILKP
Sbjct: 430 LPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGFSSRGPNTIAVDILKP 489

Query: 492 DLAAPGIDILAAYTKLSSLTGKIADSRYSLFSVMAGTSMACPHATAAAAYVKSFHPDWSP 551
           D+ APG++ILAA++ L+S      D+R+  +SV++GTSM+CPH    AAY+K+FHP+WSP
Sbjct: 490 DVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVAGVAAYIKTFHPEWSP 549

Query: 552 AAVKSALMTTATPMKIKS---EDAEFGSGAGQINPTKAVHPGLVYDISLGSYISFLCKEG 611
           + ++SA+MTTA PM          EF  GAG ++P  A++PGLVY+I    +I+FLC   
Sbjct: 550 SMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVYEIGKSDHIAFLCGLN 609

Query: 612 YNSTTIGLLVGSKEYNCSKIKPAKGTDGLNYPTMHKQLSDPGSAITAVFYRTVTDVEHGA 671
           YN+T++ L+ G       K  P      LNYP+M  +L    S+    F RTVT+V    
Sbjct: 610 YNATSLKLIAGEAVTCTGKTLPR----NLNYPSMSAKLPKSESSFIVTFNRTVTNVGTPN 669

Query: 672 SVYRANISSPVG--LSVKVFPDSLDFAKAGERKRFKVVVKGEAMRDGTRILSALLEWKDS 719
           S Y++ I    G  L V+V P  L      E++ F V V G  + D     SA L W D 
Sbjct: 670 STYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNI-DPKLPSSANLIWSDG 706