Cp4.1LG19g02490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g02490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAuxin efflux carrier component
LocationCp4.1LG19: 2090930 .. 2093575 (+)
RNA-Seq ExpressionCp4.1LG19g02490
SyntenyCp4.1LG19g02490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGACGGCATAATGTCCTGAAGAATCTCATGAAGCTCCTGGACTCTCACTTTCCTCAGAATCACTTTATTTTCTCTTTTTCATTCTGAAACACTTAAAAGTTCCTAAAAAAATCAGCTTTTTTCTCCCCTCACTCTGTCCGCCATTACAATACCTCCATAAATACACCTCCCCACCTCCCCGTTTCCCCCGGACTTTATCTTCTTCTTCCTCCTCCGCCGAATGGGTTAACCCGAAGAAACAGAGCATCTTAAACAACAAACCACAATGATCTCTGTTTCCGACCTCTACCATGTCCTCACGGCTGTCGTCCCTCTCTACGTCGCCATGATCCTAGCCTACGGTTCTGTCAAATGGTGGAAAATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGCTTCGTCGCACTATTCGCTGTCCCCCTCCTCTCCTTCCACTTCATTTCCTCCAACAACCCCTTCTCCATGAACTTCCGATTCATCGCCGCCGATACCCTTCAAAAGCTGATCGTCCTCGCCGCCCTCGCCGTTTGGTCGCATCTCTCCTCGAAGGGCTCTCTAGAATGGGCGATTACTCTGTTTTCACTCTCGACTCTGCCCAATACTCTGGTTATGGGAATCCCCCTTTTGAAGGGTATGTACGGCGACTCCACCGGTACTCTCATGGTCCAAATCGTTGTTCTTCAGTGCATCATTTGGTACACATTGATGCTGTTTTTGTTTGAGTACAGGGGAGCTAGATTGTTGATTGCAGAGCAGTTTCCTGATACTGCAGGGGAGATTGTTTCGTTTAGAGTTGATTCGGATATTTTGTCTTTGGATGGGAAAGAGCCGCTGCAGACGGAGGCGGAAATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAATCCGCGAGTTCTCGATCGGAAGTTTTTTCGCGGCGGTCGCATGGAGGAGGTGGTGGCGGCGGAGCTTCGTTGACGCCTCGGCCGTCGAATTTGACGAATGCGGAGATTTATTCTCTGCAATCTTCGAGGAATCCGACTCCGAGGGGCTCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCTAATTATGGGAACCTTGGGAATTTTGATGAGGAAAGTGGTGGATTCAAAGGGAGGGCGATGAATGGTAATGTTAATGGGTATCCGGCGCCGGCGAGCGGTGGGCTTTTTTCTCCGATGACCGGACCAGCGGTGGCGGCGGCGGCGAAGAAAAGAGTCCATGGTGGAGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCAAGTGCGTCGCCGGTTTCTGAGGGTGGGTTGAACGTTTTCCGGGGTGGGGACTACGACGGCGCCGGCGCCGGCGGGATGAACCAGAAAGGTACGGAGTTAAGATTTTGGTTTCTTGTTCTTTTTTGTGTTGGATTTTTGTACTTTTTTCAAGTGGGTCACTAAAGAATGTTTTCTCCTGTTTTTTTTTTTCCGAGTTTGTCCCATTTTTTGTCAAAACGAGCTGCATTTTTTCAGGGTATTGATCTGTTTATGCTGAAATTTTAGATTTTGATGAGTTTGGTCGGGATGAGTTCAGCTTTGGGAACAAATCCGCCGTGAACGGCGGAGGACACGACGGTGGGCCAGTGTTATCCAAGCTTGGATCCAGCTCCACCGCCGAGCTCCATCCGAAATCCGGAGACGACACGGCGGAGTCCAAGCCAACTGCCATGCCGCCGGCAAGTGTGATGACAAGGTTGATTCTGATCATGGTTTGGAGGAAACTGATCAGAAATCCCAATACCTATTCTAGCTTGATTGGCCTTGCTTGGTCTTTGATCTCCTTTAGGTGGAACATTGTAATGCCAGCAATCGTTGCTCGATCCATCGCTATTTTATCCGATGCCGGGCTCGGGATGGCAATGTTTAGTCTCGGTATCGACTTTCTTTCAATCTCAAACACTTTCAATCATACACGTAAACTATTGTATCTTCAACTATGTTATGTTTTGGGATCGTATGTAGGGTTGTTCATGGCATTGCAGCCTAAGATCATTGCCTGTGGAAACACAATCGCTACATTTGCAATGGCGGTTCGGTTCATCACCGGTCCGGCGGTGATGGCAGCTGCCTCCATTGCCGTTGGGCTTAAAGGAGTGCTGCTTCACATCGCCATAGTTCAGGTGAAGAAAATGAAACAAACACAATCCGTTTAAGAATTTAATTTGATGTCACTAACGTATGTTTCATTCGTAGGCTGCTCTCCCACAAGGGATAGTCCCTTTCGTATTTGCCAAGGAATACAACGTTCATCCCGACATATTAAGCACCGGGTTCGATCTCAATCTCAATCTTAGGCTCGATAATACCAATTATTCGTTATATTTTACATGAATTTTGGAATTTTTTTGTTTGAATCGTGTGTTGCAGTGTTATATTTGGGATGCTGATAGCGCTACCGATAACGTTGGTTTATTACATCTTATTGGGACTTTGAAGCGGGAAAGTGCGACGATGGACGAGAAATTAAAGAAGATGAGGACTACTAAGCCTTTTTTGTTGTAATTTTTTGGCCTCTTTTTTGTGCTCTATTTCTTTTGTTCTTTTATGGGAAGTAAATCAAAGCTTTTTTCAAATGGATTCTCAACCACTCT

mRNA sequence

AGACGGCATAATGTCCTGAAGAATCTCATGAAGCTCCTGGACTCTCACTTTCCTCAGAATCACTTTATTTTCTCTTTTTCATTCTGAAACACTTAAAAGTTCCTAAAAAAATCAGCTTTTTTCTCCCCTCACTCTGTCCGCCATTACAATACCTCCATAAATACACCTCCCCACCTCCCCGTTTCCCCCGGACTTTATCTTCTTCTTCCTCCTCCGCCGAATGGGTTAACCCGAAGAAACAGAGCATCTTAAACAACAAACCACAATGATCTCTGTTTCCGACCTCTACCATGTCCTCACGGCTGTCGTCCCTCTCTACGTCGCCATGATCCTAGCCTACGGTTCTGTCAAATGGTGGAAAATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGCTTCGTCGCACTATTCGCTGTCCCCCTCCTCTCCTTCCACTTCATTTCCTCCAACAACCCCTTCTCCATGAACTTCCGATTCATCGCCGCCGATACCCTTCAAAAGCTGATCGTCCTCGCCGCCCTCGCCGTTTGGTCGCATCTCTCCTCGAAGGGCTCTCTAGAATGGGCGATTACTCTGTTTTCACTCTCGACTCTGCCCAATACTCTGGTTATGGGAATCCCCCTTTTGAAGGGTATGTACGGCGACTCCACCGGTACTCTCATGGTCCAAATCGTTGTTCTTCAGTGCATCATTTGGTACACATTGATGCTGTTTTTGTTTGAGTACAGGGGAGCTAGATTGTTGATTGCAGAGCAGTTTCCTGATACTGCAGGGGAGATTGTTTCGTTTAGAGTTGATTCGGATATTTTGTCTTTGGATGGGAAAGAGCCGCTGCAGACGGAGGCGGAAATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAATCCGCGAGTTCTCGATCGGAAGTTTTTTCGCGGCGGTCGCATGGAGGAGGTGGTGGCGGCGGAGCTTCGTTGACGCCTCGGCCGTCGAATTTGACGAATGCGGAGATTTATTCTCTGCAATCTTCGAGGAATCCGACTCCGAGGGGCTCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCTAATTATGGGAACCTTGGGAATTTTGATGAGGAAAGTGGTGGATTCAAAGGGAGGGCGATGAATGGTAATGTTAATGGGTATCCGGCGCCGGCGAGCGGTGGGCTTTTTTCTCCGATGACCGGACCAGCGGTGGCGGCGGCGGCGAAGAAAAGAGTCCATGGTGGAGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCAAGTGCGTCGCCGGTTTCTGAGGGTGGGTTGAACGTTTTCCGGGGTGGGGACTACGACGGCGCCGGCGCCGGCGGGATGAACCAGAAAGATTTTGATGAGTTTGGTCGGGATGAGTTCAGCTTTGGGAACAAATCCGCCGTGAACGGCGGAGGACACGACGGTGGGCCAGTGTTATCCAAGCTTGGATCCAGCTCCACCGCCGAGCTCCATCCGAAATCCGGAGACGACACGGCGGAGTCCAAGCCAACTGCCATGCCGCCGGCAAGTGTGATGACAAGGTTGATTCTGATCATGGTTTGGAGGAAACTGATCAGAAATCCCAATACCTATTCTAGCTTGATTGGCCTTGCTTGGTCTTTGATCTCCTTTAGGTGGAACATTGTAATGCCAGCAATCGTTGCTCGATCCATCGCTATTTTATCCGATGCCGGGCTCGGGATGGCAATGTTTAGTCTCGGGTTGTTCATGGCATTGCAGCCTAAGATCATTGCCTGTGGAAACACAATCGCTACATTTGCAATGGCGGTTCGGTTCATCACCGGTCCGGCGGTGATGGCAGCTGCCTCCATTGCCGTTGGGCTTAAAGGAGTGCTGCTTCACATCGCCATAGTTCAGGCTGCTCTCCCACAAGGGATAGTCCCTTTCGTATTTGCCAAGGAATACAACGTTCATCCCGACATATTAAGCACCGGTGTTATATTTGGGATGCTGATAGCGCTACCGATAACGTTGGTTTATTACATCTTATTGGGACTTTGAAGCGGGAAAGTGCGACGATGGACGAGAAATTAAAGAAGATGAGGACTACTAAGCCTTTTTTGTTGTAATTTTTTGGCCTCTTTTTTGTGCTCTATTTCTTTTGTTCTTTTATGGGAAGTAAATCAAAGCTTTTTTCAAATGGATTCTCAACCACTCT

Coding sequence (CDS)

ATGATCTCTGTTTCCGACCTCTACCATGTCCTCACGGCTGTCGTCCCTCTCTACGTCGCCATGATCCTAGCCTACGGTTCTGTCAAATGGTGGAAAATCTTCTCCCCGGACCAATGCTCCGGCATCAACCGCTTCGTCGCACTATTCGCTGTCCCCCTCCTCTCCTTCCACTTCATTTCCTCCAACAACCCCTTCTCCATGAACTTCCGATTCATCGCCGCCGATACCCTTCAAAAGCTGATCGTCCTCGCCGCCCTCGCCGTTTGGTCGCATCTCTCCTCGAAGGGCTCTCTAGAATGGGCGATTACTCTGTTTTCACTCTCGACTCTGCCCAATACTCTGGTTATGGGAATCCCCCTTTTGAAGGGTATGTACGGCGACTCCACCGGTACTCTCATGGTCCAAATCGTTGTTCTTCAGTGCATCATTTGGTACACATTGATGCTGTTTTTGTTTGAGTACAGGGGAGCTAGATTGTTGATTGCAGAGCAGTTTCCTGATACTGCAGGGGAGATTGTTTCGTTTAGAGTTGATTCGGATATTTTGTCTTTGGATGGGAAAGAGCCGCTGCAGACGGAGGCGGAAATTGGGGAAGATGGGAAGTTGAGAGTGGTGGTGAGGAAATCCGCGAGTTCTCGATCGGAAGTTTTTTCGCGGCGGTCGCATGGAGGAGGTGGTGGCGGCGGAGCTTCGTTGACGCCTCGGCCGTCGAATTTGACGAATGCGGAGATTTATTCTCTGCAATCTTCGAGGAATCCGACTCCGAGGGGCTCGAGTTTTAATCATTCGGATTTCTTTTTGGGGAATGCGAGTCCGAGGCATTCTAATTATGGGAACCTTGGGAATTTTGATGAGGAAAGTGGTGGATTCAAAGGGAGGGCGATGAATGGTAATGTTAATGGGTATCCGGCGCCGGCGAGCGGTGGGCTTTTTTCTCCGATGACCGGACCAGCGGTGGCGGCGGCGGCGAAGAAAAGAGTCCATGGTGGAGATGGGGGAAAGGATTTGCATATGTTTGTTTGGAGTTCAAGTGCGTCGCCGGTTTCTGAGGGTGGGTTGAACGTTTTCCGGGGTGGGGACTACGACGGCGCCGGCGCCGGCGGGATGAACCAGAAAGATTTTGATGAGTTTGGTCGGGATGAGTTCAGCTTTGGGAACAAATCCGCCGTGAACGGCGGAGGACACGACGGTGGGCCAGTGTTATCCAAGCTTGGATCCAGCTCCACCGCCGAGCTCCATCCGAAATCCGGAGACGACACGGCGGAGTCCAAGCCAACTGCCATGCCGCCGGCAAGTGTGATGACAAGGTTGATTCTGATCATGGTTTGGAGGAAACTGATCAGAAATCCCAATACCTATTCTAGCTTGATTGGCCTTGCTTGGTCTTTGATCTCCTTTAGGTGGAACATTGTAATGCCAGCAATCGTTGCTCGATCCATCGCTATTTTATCCGATGCCGGGCTCGGGATGGCAATGTTTAGTCTCGGGTTGTTCATGGCATTGCAGCCTAAGATCATTGCCTGTGGAAACACAATCGCTACATTTGCAATGGCGGTTCGGTTCATCACCGGTCCGGCGGTGATGGCAGCTGCCTCCATTGCCGTTGGGCTTAAAGGAGTGCTGCTTCACATCGCCATAGTTCAGGCTGCTCTCCCACAAGGGATAGTCCCTTTCGTATTTGCCAAGGAATACAACGTTCATCCCGACATATTAAGCACCGGTGTTATATTTGGGATGCTGATAGCGCTACCGATAACGTTGGTTTATTACATCTTATTGGGACTTTGA

Protein sequence

MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFISSNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPLLKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSDILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Homology
BLAST of Cp4.1LG19g02490 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 1.0e-206
Identity = 414/610 (67.87%), Postives = 476/610 (78.03%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWW+IF+PDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNP++MN RFIAADTLQKLIVLA L +WSHLS +GSLEW ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G+LMVQIVVLQCIIWYTLMLF+FEYRGAR+LI EQFPDTAG I S  VD+D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFPDTAGAIASIVVDAD 180

Query: 181 ILSLDG-KEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNL 240
           ++SLDG ++ ++TEAE+ EDGK+ V VR+S +SRS+V+SRRS      G +S TPRPSNL
Sbjct: 181 VVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSM-----GFSSTTPRPSNL 240

Query: 241 TNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNV 300
           TNAEIYSLQSSRNPTPRGSSFNH+DF+  +   R SN+     F   +G    R  N   
Sbjct: 241 TNAEIYSLQSSRNPTPRGSSFNHTDFY--SMVGRSSNFAAGDAFGVRTGA-TPRPSNYEE 300

Query: 301 NGYPAPASGGLFS--PMTGPAVAA--AAKKRVHG---GDGGKDLHMFVWSSSASPVSEGG 360
           +      +G  +   P   PA+AA    KK  +G   G+ GKDLHMFVWSSSASPVS+  
Sbjct: 301 DAAAPNKAGSKYGQYPAPNPAMAAPPKPKKAANGQAKGEDGKDLHMFVWSSSASPVSD-- 360

Query: 361 LNVF-RGGDYDGAGA------GGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLG 420
             VF  G +Y+ A A         + +  D   RD+FSFGN+         G     K  
Sbjct: 361 --VFGNGAEYNDAAAVKEVRMAVASPRKADGVERDDFSFGNRGVAERDAEAGD---EKSV 420

Query: 421 SSSTAELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLIS 480
           +++ +  H K G   A   PTAMPP SVMTRLILIMVWRKLIRNPNTYSSLIGL WSL+ 
Sbjct: 421 AAAVSGEHGKPGLTPA---PTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVC 480

Query: 481 FRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGP 540
           FRWN  MPAI+ +SI+ILSDAGLGMAMFSLGLFMALQP+IIACGN +ATFAMAVRF+TGP
Sbjct: 481 FRWNFEMPAIILKSISILSDAGLGMAMFSLGLFMALQPRIIACGNKVATFAMAVRFLTGP 540

Query: 541 AVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPI 596
           AVMAAASIAVGL+G LLH+AIVQAALPQGIVPFVFAKEY+VHPDILST VIFGMLIALPI
Sbjct: 541 AVMAAASIAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPDILSTAVIFGMLIALPI 592

BLAST of Cp4.1LG19g02490 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 2.0e-205
Identity = 412/619 (66.56%), Postives = 478/619 (77.22%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWW+IF+PDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNP++MN RFIAADTLQKL+VLA L  WSHLS +GSLEW ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G+LMVQIVVLQCIIWYTLMLF+FEYRGAR+LI EQFPDTA  I S  VD D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFPDTAANIASIVVDPD 180

Query: 181 ILSLDG-KEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNL 240
           ++SLDG ++ ++TE E+ EDG++ V VR+S +SRS+++SRRS      G +S TPRPSNL
Sbjct: 181 VVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIYSRRSM-----GFSSTTPRPSNL 240

Query: 241 TNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGF---KGRAMN 300
           TNAEIYSLQSSRNPTPRGSSFNH+DF+  +   R SN+G    F   +G          +
Sbjct: 241 TNAEIYSLQSSRNPTPRGSSFNHTDFY--SMVGRSSNFGAADAFGVRTGATPRPSNYEDD 300

Query: 301 GNVNGYPAPASGGLFSPMTG------PAVAAA---AKKRVHGGDG-GKDLHMFVWSSSAS 360
            +   YP PAS    +PM G      PAV++A   AKK    G   G+DLHMFVWSSSAS
Sbjct: 301 ASKPKYPLPASNA--APMAGHYPAPNPAVSSAPKGAKKAATNGQAKGEDLHMFVWSSSAS 360

Query: 361 PVSEGGLNVFRGG--DYDGAGA--------GGMNQKDFDEFGRDEFSFGNKSAVNGGGHD 420
           PVS+    VF GG  DY+ A A        G  +++D+ E  RD+FSFGN+  ++     
Sbjct: 361 PVSD----VFGGGAPDYNDAAAVKSPRKMDGAKDREDYVE--RDDFSFGNRGVMDRDAEA 420

Query: 421 GGPVLSKLGSSSTAELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSL 480
           G     K  +++ A+         A + PTAMPP SVMTRLILIMVWRKLIRNPNTYSSL
Sbjct: 421 GD---EKAAAAAGAD------PSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSL 480

Query: 481 IGLAWSLISFRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFA 540
           IGL WSL+ FRWN  MPAIV +SI+ILSDAGLGMAMFSLGLFMALQP IIACGN +AT+A
Sbjct: 481 IGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIACGNKVATYA 540

Query: 541 MAVRFITGPAVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVI 596
           MAVRF+ GPAVMAAAS AVGL+G LLH+AIVQAALPQGIVPFVFAKEY+VHP ILST VI
Sbjct: 541 MAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPSILSTAVI 595

BLAST of Cp4.1LG19g02490 vs. ExPASy Swiss-Prot
Match: Q0IQA5 (Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1D PE=2 SV=2)

HSP 1 Score: 715.7 bits (1846), Expect = 4.4e-205
Identity = 421/614 (68.57%), Postives = 467/614 (76.06%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+V DLYHVLTAVVPLYVAM LAY SV+WW+IFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNPF+MN RF+AADTLQKLIVLA LA+W  LS++GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMY-------GDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIV 180
           LKGMY       G  +G+LMVQIVVLQCIIWYTLMLFLFEYRGARLL+ EQFPDTA  IV
Sbjct: 121 LKGMYAAAGAAAGADSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIV 180

Query: 181 SFRVDSDILSL----DGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGG 240
           SFRVDSD++SL     G   LQ EAE+G+DGK+RV VRKS SSRSE  +  SH G     
Sbjct: 181 SFRVDSDVVSLAGGGGGAAELQAEAEVGDDGKMRVTVRKSTSSRSE--AACSH-GTQSHS 240

Query: 241 ASLTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGG 300
            S+ PR SNL+  EIYSLQSSRNPTPRGSSFNH++FF        +  GN  + DEE G 
Sbjct: 241 QSMQPRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFF--------NIVGNGKHGDEEKGA 300

Query: 301 FKGRAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVS 360
                           A GG  SP   P V     KR       KDLHMFVWSSSASPVS
Sbjct: 301 ----------------AGGGGHSPQ--PVVG----KR-------KDLHMFVWSSSASPVS 360

Query: 361 E--------GGLNVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVL 420
           E        G ++VF GG  D   A G           DE+SFGNK+         GP L
Sbjct: 361 ERAAAAAAAGAVHVFGGGGADHGDAKGAQ-------AYDEYSFGNKN------EKDGPTL 420

Query: 421 SKLGSSSTAELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAW 480
           SKLGS+STA+L PK   D  E +  AMPPASVMTRLILIMVWRKLIRNPNTYSSL+G+ W
Sbjct: 421 SKLGSNSTAQLRPK---DDGEGRAAAMPPASVMTRLILIMVWRKLIRNPNTYSSLLGVIW 480

Query: 481 SLISFRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRF 540
           SL+S+RW I MPAI+ARSI+ILSDAGLGMAMFSLGLFMALQP+IIACGN++A++AMAVRF
Sbjct: 481 SLVSYRWGIEMPAIIARSISILSDAGLGMAMFSLGLFMALQPRIIACGNSLASYAMAVRF 540

Query: 541 ITGPAVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLI 596
           + GPAVMAAASIAVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHP+ILST VIFGMLI
Sbjct: 541 LVGPAVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPNILSTAVIFGMLI 558

BLAST of Cp4.1LG19g02490 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 2.8e-204
Identity = 416/651 (63.90%), Postives = 481/651 (73.89%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWWKIF+PDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNP++MN RF+AAD+LQK+IVL+ L +W  LS  GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G LMVQIVVLQCIIWYTLMLFLFEYRGA+LLI+EQFPDTAG IVS  VDSD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           I+SLDG++PL+TEAEI EDGKL V VR+S +SRS+++SRRS       G S TPRPSNLT
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQ------GLSATPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNYG-------------NLGNFDE 300
           NAEIYSLQSSRNPTPRGSSFNH+DF+  + +   R+SN+G                N++E
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEE 300

Query: 301 ESGGFK-----------------GRAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKR-- 360
           + G  K                 G +  G    YPAP + G+FSP TG     AAK    
Sbjct: 301 DGGPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAP-NPGMFSPNTGGGGGTAAKGNAP 360

Query: 361 VHGG---DG-GKDLHMFVWSSSASPVSEGGLNVFRGG------DYDGA------------ 420
           V GG   DG G+DLHMFVWSSSASPVS+    VF GG      DY  A            
Sbjct: 361 VVGGKRQDGNGRDLHMFVWSSSASPVSD----VFGGGGGNHHADYSTATNDHQKDVKISV 420

Query: 421 GAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDTAESK 480
             G  N   + E  R+EFSFGNK        D   VL+  G ++ +       + T ++K
Sbjct: 421 PQGNSNDNQYVE--REEFSFGNKD-------DDSKVLATDGGNNIS-------NKTTQAK 480

Query: 481 PTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSIAILS 540
              MPP SVMTRLILIMVWRKLIRNPN+YSSL G+ WSLISF+WNI MPA++A+SI+ILS
Sbjct: 481 --VMPPTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILS 540

Query: 541 DAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGVLLHI 596
           DAGLGMAMFSLGLFMAL P+IIACGN  A FA A+RF+ GPAVM  AS AVGL+GVLLH+
Sbjct: 541 DAGLGMAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHV 600

BLAST of Cp4.1LG19g02490 vs. ExPASy Swiss-Prot
Match: P0C0X5 (Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1B PE=2 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.7e-204
Identity = 419/610 (68.69%), Postives = 465/610 (76.23%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+V DLYHVLTAVVPLYVAM LAY SV+WW+IFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MITVVDLYHVLTAVVPLYVAMTLAYASVRWWRIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNPF+MN RF+AADTLQKLIVLA LA+W  LS++GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFAMNLRFLAADTLQKLIVLALLALWCRLSARGSLDWLITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDS----TGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFR 180
           LKGMY  +    +G+LMVQIVVLQCIIWYTLMLFLFEYRGARLL+ EQFPDTA  IVSFR
Sbjct: 121 LKGMYAAAADVDSGSLMVQIVVLQCIIWYTLMLFLFEYRGARLLVMEQFPDTAASIVSFR 180

Query: 181 VDSDILSL----DGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASL 240
           VDSD++SL     G   LQ EAE+G+DG++RV VRKS SSRSE  +  SH G      S+
Sbjct: 181 VDSDVVSLAGGGGGAAELQAEAEVGDDGRMRVTVRKSTSSRSE--AACSH-GTQSHSQSM 240

Query: 241 TPRPSNLTNAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKG 300
            PR SNL+  EIYSLQSSRNPTPRGSSFNH++FF        +  GN    DEE G    
Sbjct: 241 QPRVSNLSGVEIYSLQSSRNPTPRGSSFNHAEFF--------NIVGNGKQGDEEKGA--- 300

Query: 301 RAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSE-- 360
                        A GG  SP   P V     KR       KDLHMFVWSSSASPVSE  
Sbjct: 301 -------------AGGGGHSPQ--PVVG----KR-------KDLHMFVWSSSASPVSERA 360

Query: 361 -----GGLNVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLG 420
                G ++VF GG  D   A G           DE+SFGNK+         GP LSKLG
Sbjct: 361 AAAAAGAVHVFGGGGADHGDAKGAQ-------AYDEYSFGNKN------EKDGPTLSKLG 420

Query: 421 SSSTAELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLIS 480
           S+STA+L PK   D  E    AMPPASVMTRLILIMVWRKLIRNPNTYSSL+G+ WSL+S
Sbjct: 421 SNSTAQLRPK---DDGEGMAAAMPPASVMTRLILIMVWRKLIRNPNTYSSLLGVIWSLVS 480

Query: 481 FRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGP 540
           +RW I MPAI+ARSI+ILSDAGLGMAMFSLGLFMALQP+IIACGN++A++AMAVRF+ GP
Sbjct: 481 YRWGIEMPAIIARSISILSDAGLGMAMFSLGLFMALQPRIIACGNSLASYAMAVRFLVGP 540

Query: 541 AVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPI 596
           AVMAAASIAVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHP+ILST VIFGMLIALPI
Sbjct: 541 AVMAAASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPNILSTAVIFGMLIALPI 554

BLAST of Cp4.1LG19g02490 vs. NCBI nr
Match: XP_023518356.1 (probable auxin efflux carrier component 1b [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135 bits (2936), Expect = 0.0
Identity = 595/595 (100.00%), Postives = 595/595 (100.00%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300

Query: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360
           GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD
Sbjct: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360

Query: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420
           YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT
Sbjct: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420

Query: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480
           AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI
Sbjct: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480

Query: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540
           AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV
Sbjct: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540

Query: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595
           LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595

BLAST of Cp4.1LG19g02490 vs. NCBI nr
Match: XP_022966088.1 (probable auxin efflux carrier component 1b [Cucurbita maxima])

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 591/595 (99.33%), Postives = 592/595 (99.50%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGN GNFDEESGGFKGRA+NGNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNFGNFDEESGGFKGRAINGNVN 300

Query: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360
           GYPAPASGGLFSPMTGPA  AAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD
Sbjct: 301 GYPAPASGGLFSPMTGPA--AAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360

Query: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420
           YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT
Sbjct: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420

Query: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480
           AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI
Sbjct: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480

Query: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540
           AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV
Sbjct: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540

Query: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595
           LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 593

BLAST of Cp4.1LG19g02490 vs. NCBI nr
Match: XP_022931834.1 (probable auxin efflux carrier component 1b [Cucurbita moschata])

HSP 1 Score: 1123 bits (2905), Expect = 0.0
Identity = 590/595 (99.16%), Postives = 592/595 (99.50%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGN GNFDEESGGFKGRA+NGNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNFGNFDEESGGFKGRAINGNVN 300

Query: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360
           GYPAPASGGLFSPMTGPA AAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD
Sbjct: 301 GYPAPASGGLFSPMTGPA-AAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360

Query: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420
           YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT
Sbjct: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420

Query: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480
           AESKPT MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI+MPAIVARSI
Sbjct: 421 AESKPTTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIMMPAIVARSI 480

Query: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540
           AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV
Sbjct: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540

Query: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595
           LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 594

BLAST of Cp4.1LG19g02490 vs. NCBI nr
Match: XP_038880940.1 (probable auxin efflux carrier component 1b isoform X2 [Benincasa hispida])

HSP 1 Score: 1062 bits (2746), Expect = 0.0
Identity = 564/606 (93.07%), Postives = 578/606 (95.38%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNPFSMN RFIAADTLQKLIVLAALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSHGGGGG   SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGG--VSLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GN GNFDEE+GG KGR ++GNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVN 300

Query: 301 ---------GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEG 360
                    GYPAPAS GLFSP+TGPA   AAKKRV+GGDGGKDLHMFVWSSSASPVSEG
Sbjct: 301 VNGVFPSSTGYPAPASAGLFSPVTGPA---AAKKRVNGGDGGKDLHMFVWSSSASPVSEG 360

Query: 361 GLNVFRGGDYDGAGAGG--MNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSST 420
           G+NVFR GDYDG G GG  MN+KDFD+FGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSST
Sbjct: 361 GINVFRSGDYDGGGGGGGGMNRKDFDDFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSST 420

Query: 421 AELHPKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWN 480
            ELHPK+GDDTAESKPT MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWN
Sbjct: 421 TELHPKNGDDTAESKPTTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWN 480

Query: 481 IVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMA 540
           IVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMA
Sbjct: 481 IVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMA 540

Query: 541 AASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVY 595
           AASIAVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVY
Sbjct: 541 AASIAVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVY 600

BLAST of Cp4.1LG19g02490 vs. NCBI nr
Match: XP_008455726.1 (PREDICTED: probable auxin efflux carrier component 1b [Cucumis melo])

HSP 1 Score: 1060 bits (2740), Expect = 0.0
Identity = 563/602 (93.52%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMN RFIAADTLQKLIVLAALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSHGGGGG   SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGG--VSLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GN GNFDEE+GG K R ++GNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVN 300

Query: 301 G-------YPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGL 360
           G       YPAPASGGLFSP+TGPA   A KKR +GGDGGKDLHMFVWSSSASPVSEGG+
Sbjct: 301 GIFPSSNGYPAPASGGLFSPVTGPA---ATKKR-NGGDGGKDLHMFVWSSSASPVSEGGI 360

Query: 361 NVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420
           NVFR GDYDG GAGGMNQKDF+E+G DEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH
Sbjct: 361 NVFRSGDYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420

Query: 421 PKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMP 480
           PK+GDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MP
Sbjct: 421 PKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMP 480

Query: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASI 540
           AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMAAASI
Sbjct: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASI 540

Query: 541 AVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 595
           AVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL
Sbjct: 541 AVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 596

BLAST of Cp4.1LG19g02490 vs. ExPASy TrEMBL
Match: A0A6J1HQV8 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465879 PE=3 SV=1)

HSP 1 Score: 1124 bits (2907), Expect = 0.0
Identity = 591/595 (99.33%), Postives = 592/595 (99.50%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGN GNFDEESGGFKGRA+NGNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNFGNFDEESGGFKGRAINGNVN 300

Query: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360
           GYPAPASGGLFSPMTGPA  AAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD
Sbjct: 301 GYPAPASGGLFSPMTGPA--AAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360

Query: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420
           YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT
Sbjct: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420

Query: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480
           AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI
Sbjct: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480

Query: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540
           AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV
Sbjct: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540

Query: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595
           LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 593

BLAST of Cp4.1LG19g02490 vs. ExPASy TrEMBL
Match: A0A6J1EVC1 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111438127 PE=3 SV=1)

HSP 1 Score: 1123 bits (2905), Expect = 0.0
Identity = 590/595 (99.16%), Postives = 592/595 (99.50%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGN GNFDEESGGFKGRA+NGNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNFGNFDEESGGFKGRAINGNVN 300

Query: 301 GYPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360
           GYPAPASGGLFSPMTGPA AAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD
Sbjct: 301 GYPAPASGGLFSPMTGPA-AAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGLNVFRGGD 360

Query: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420
           YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT
Sbjct: 361 YDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDT 420

Query: 421 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 480
           AESKPT MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI+MPAIVARSI
Sbjct: 421 AESKPTTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIMMPAIVARSI 480

Query: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540
           AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV
Sbjct: 481 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 540

Query: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 595
           LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL
Sbjct: 541 LLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILLGL 594

BLAST of Cp4.1LG19g02490 vs. ExPASy TrEMBL
Match: A0A1S3C1I4 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103495829 PE=3 SV=1)

HSP 1 Score: 1060 bits (2740), Expect = 0.0
Identity = 563/602 (93.52%), Postives = 577/602 (95.85%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMN RFIAADTLQKLIVLAALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLAALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSHGGGGG   SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGG--VSLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GN GNFDEE+GG K R ++GNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGLKSRGISGNVN 300

Query: 301 G-------YPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGL 360
           G       YPAPASGGLFSP+TGPA   A KKR +GGDGGKDLHMFVWSSSASPVSEGG+
Sbjct: 301 GIFPSSNGYPAPASGGLFSPVTGPA---ATKKR-NGGDGGKDLHMFVWSSSASPVSEGGI 360

Query: 361 NVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420
           NVFR GDYDG GAGGMNQKDF+E+G DEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH
Sbjct: 361 NVFRSGDYDGPGAGGMNQKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420

Query: 421 PKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMP 480
           PK+GDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MP
Sbjct: 421 PKNGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMP 480

Query: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASI 540
           AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMAAASI
Sbjct: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASI 540

Query: 541 AVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 595
           AVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL
Sbjct: 541 AVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 596

BLAST of Cp4.1LG19g02490 vs. ExPASy TrEMBL
Match: A0A0A0KYF4 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_4G430820 PE=3 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 561/602 (93.19%), Postives = 574/602 (95.35%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMN RFIAADTLQKLIVL ALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSHGGGGG   SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGG--VSLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GN GNFDEE+GG KGR ++GNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVN 300

Query: 301 G-------YPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGL 360
           G       YPAPASGGLFSP+TGPA   A KKR +GGDGGKDLHMFVWSSSASPVSEGG+
Sbjct: 301 GIFPSANGYPAPASGGLFSPVTGPA---ATKKR-NGGDGGKDLHMFVWSSSASPVSEGGI 360

Query: 361 NVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420
           NVFR GDYD  GAGGMN KDF+E+G DEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH
Sbjct: 361 NVFRSGDYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420

Query: 421 PKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMP 480
           PK GDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MP
Sbjct: 421 PKHGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMP 480

Query: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASI 540
           AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMAAASI
Sbjct: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASI 540

Query: 541 AVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 595
           AVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL
Sbjct: 541 AVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 596

BLAST of Cp4.1LG19g02490 vs. ExPASy TrEMBL
Match: D7URM4 (Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=CsPIN4 PE=2 SV=1)

HSP 1 Score: 1054 bits (2726), Expect = 0.0
Identity = 561/602 (93.19%), Postives = 573/602 (95.18%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS
Sbjct: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNPFSMN RFIAADTLQKLIVL ALAVWSHLSSKGSLEW+ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNNPFSMNLRFIAADTLQKLIVLVALAVWSHLSSKGSLEWSITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD
Sbjct: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ILSLDGKEPLQTEAEIGEDGKLRVVVRKS SSRSEVFSRRSHGGGGG   SLTPRPSNLT
Sbjct: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSTSSRSEVFSRRSHGGGGG--VSLTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNYGNLGNFDEESGGFKGRAMNGNVN 300
           NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSN+GN GNFDEE+GG KGR ++GNVN
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHSDFFLGNASPRHSNFGNFGNFDEENGGMKGRGISGNVN 300

Query: 301 G-------YPAPASGGLFSPMTGPAVAAAAKKRVHGGDGGKDLHMFVWSSSASPVSEGGL 360
           G       YPAPASGGLFSP TGPA   A KKR +GGDGGKDLHMFVWSSSASPVSEGG+
Sbjct: 301 GIFPSANGYPAPASGGLFSPATGPA---ATKKR-NGGDGGKDLHMFVWSSSASPVSEGGI 360

Query: 361 NVFRGGDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420
           NVFR GDYD  GAGGMN KDF+E+G DEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH
Sbjct: 361 NVFRSGDYDAPGAGGMNPKDFNEYGGDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELH 420

Query: 421 PKSGDDTAESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMP 480
           PK GDDTAESKPT+MPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNI MP
Sbjct: 421 PKHGDDTAESKPTSMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIAMP 480

Query: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASI 540
           AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIA+FAMAVRFITGPAVMAAASI
Sbjct: 481 AIVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIASFAMAVRFITGPAVMAAASI 540

Query: 541 AVGLKGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 595
           AVGL+GVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL
Sbjct: 541 AVGLRGVLLHIAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYILL 596

BLAST of Cp4.1LG19g02490 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 713.0 bits (1839), Expect = 2.0e-205
Identity = 416/651 (63.90%), Postives = 481/651 (73.89%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+ +D YHV+TA+VPLYVAMILAYGSVKWWKIF+PDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNP++MN RF+AAD+LQK+IVL+ L +W  LS  GSL+W ITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           LKGMYG+ +G LMVQIVVLQCIIWYTLMLFLFEYRGA+LLI+EQFPDTAG IVS  VDSD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           I+SLDG++PL+TEAEI EDGKL V VR+S +SRS+++SRRS       G S TPRPSNLT
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQ------GLSATPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNYG-------------NLGNFDE 300
           NAEIYSLQSSRNPTPRGSSFNH+DF+  + +   R+SN+G                N++E
Sbjct: 241 NAEIYSLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEE 300

Query: 301 ESGGFK-----------------GRAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKR-- 360
           + G  K                 G +  G    YPAP + G+FSP TG     AAK    
Sbjct: 301 DGGPAKPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAP-NPGMFSPNTGGGGGTAAKGNAP 360

Query: 361 VHGG---DG-GKDLHMFVWSSSASPVSEGGLNVFRGG------DYDGA------------ 420
           V GG   DG G+DLHMFVWSSSASPVS+    VF GG      DY  A            
Sbjct: 361 VVGGKRQDGNGRDLHMFVWSSSASPVSD----VFGGGGGNHHADYSTATNDHQKDVKISV 420

Query: 421 GAGGMNQKDFDEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDTAESK 480
             G  N   + E  R+EFSFGNK        D   VL+  G ++ +       + T ++K
Sbjct: 421 PQGNSNDNQYVE--REEFSFGNKD-------DDSKVLATDGGNNIS-------NKTTQAK 480

Query: 481 PTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSIAILS 540
              MPP SVMTRLILIMVWRKLIRNPN+YSSL G+ WSLISF+WNI MPA++A+SI+ILS
Sbjct: 481 --VMPPTSVMTRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILS 540

Query: 541 DAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGVLLHI 596
           DAGLGMAMFSLGLFMAL P+IIACGN  A FA A+RF+ GPAVM  AS AVGL+GVLLH+
Sbjct: 541 DAGLGMAMFSLGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHV 600

BLAST of Cp4.1LG19g02490 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 671.4 bits (1731), Expect = 6.7e-193
Identity = 391/631 (61.97%), Postives = 460/631 (72.90%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNP++MN RFIAADTLQKLI+L  L +W++ +  GSLEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFEYRGA++LI EQFP+T   IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ++SLDG + L+T+A+IG+DGKL V VRKS +SR   +        GGGG ++TPRPSNLT
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFY--------GGGGTNMTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNYGNLGNFDEES--------GGF 300
            AEIYSL    N TPRGS+FNHSDF+  +G    R SN+G    +  +S          F
Sbjct: 241 GAEIYSL----NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNF 300

Query: 301 K---GRAMNGNVNGYPAPASGGLFSP----MTGPAVAAAAKKRVH------GGDGGKDLH 360
           +     A +     YP  A G   +P     TG    + A K  H        +  K+LH
Sbjct: 301 EESCAMASSPRFGYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELH 360

Query: 361 MFVWSSSASPVSE-GGLNVFRG-----GDYDGAGAGGMNQKDFDEFGRDEFSFGNKSAVN 420
           MFVW S+ SPVS+  GL V  G     G  D  GA  +     D     E   G  +   
Sbjct: 361 MFVWGSNGSPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNGENKAGPMNGDY 420

Query: 421 GGGHDGGPV------LSKLGSSSTAELHPKSGDDTAESKPTA-MPPASVMTRLILIMVWR 480
           GG  +   V      L KL  +STAEL+PK   +T E+ P   MPPASVMTRLILIMVWR
Sbjct: 421 GGEEESERVKEVPNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWR 480

Query: 481 KLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSIAILSDAGLGMAMFSLGLFMALQPK 540
           KLIRNPNTYSSLIGL W+L++FRW++ MP I+ +SI+ILSDAGLGMAMFSLGLFMALQPK
Sbjct: 481 KLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPK 540

Query: 541 IIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGVLLHIAIVQAALPQGIVPFVFAKEY 596
           +IACGN+ ATFAMAVRF TGPAVMA A++A+GL+G LL +AIVQAALPQGIVPFVFAKEY
Sbjct: 541 LIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIVPFVFAKEY 600

BLAST of Cp4.1LG19g02490 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 671.0 bits (1730), Expect = 8.8e-193
Identity = 394/639 (61.66%), Postives = 463/639 (72.46%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SNNP++MN RFIAADTLQKLI+L  L +W++ +  GSLEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFEYRGA++LI EQFP+T   IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ++SLDG + L+T+A+IG+DGKL V VRKS +SR   +        GGGG ++TPRPSNLT
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFY--------GGGGTNMTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNYGNLGNFDEES--------GGF 300
            AEIYSL    N TPRGS+FNHSDF+  +G    R SN+G    +  +S          F
Sbjct: 241 GAEIYSL----NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNF 300

Query: 301 K---GRAMNGNVNGYPAPASGGLFSP----MTGPAVAAAAKKRVH------GGDGGKDLH 360
           +     A +     YP  A G   +P     TG    + A K  H        +  K+LH
Sbjct: 301 EESCAMASSPRFGYYPGGAPGSYPAPNPEFSTGNKTGSKAPKENHHHVGKSNSNDAKELH 360

Query: 361 MFVWSSSASPVSE-GGLNVFRG-------GDYDGA------------GAGGMNQKDFDEF 420
           MFVW S+ SPVS+  GL V  G        D  GA             AG MN     ++
Sbjct: 361 MFVWGSNGSPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMN----GDY 420

Query: 421 GRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPKSGDDTAESKPTA-MPPASVMTR 480
           G +E S   K   NG        L KL  +STAEL+PK   +T E+ P   MPPASVMTR
Sbjct: 421 GGEEESERVKEVPNG--------LHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTR 480

Query: 481 LILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSIAILSDAGLGMAMFSLG 540
           LILIMVWRKLIRNPNTYSSLIGL W+L++FRW++ MP I+ +SI+ILSDAGLGMAMFSLG
Sbjct: 481 LILIMVWRKLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLG 540

Query: 541 LFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGVLLHIAIVQAALPQGIV 596
           LFMALQPK+IACGN+ ATFAMAVRF TGPAVMA A++A+GL+G LL +AIVQAALPQGIV
Sbjct: 541 LFMALQPKLIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIV 600

BLAST of Cp4.1LG19g02490 vs. TAIR 10
Match: AT5G57090.1 (Auxin efflux carrier family protein )

HSP 1 Score: 666.4 bits (1718), Expect = 2.2e-191
Identity = 393/655 (60.00%), Postives = 471/655 (71.91%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MI+  D+Y VL A+VPLYVAMILAYGSV+WW IF+PDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MITGKDMYDVLAAMVPLYVAMILAYGSVRWWGIFTPDQCSGINRFVAVFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           SN+P++MN+ F+AAD+LQK+++LAAL +W   S +GSLEW ITLFSLSTLPNTLVMGIPL
Sbjct: 61  SNDPYAMNYHFLAADSLQKVVILAALFLWQAFSRRGSLEWMITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L+ MYGD +G LMVQIVVLQ IIWYTLMLFLFE+RGA+LLI+EQFP+TAG I SFRVDSD
Sbjct: 121 LRAMYGDFSGNLMVQIVVLQSIIWYTLMLFLFEFRGAKLLISEQFPETAGSITSFRVDSD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ++SL+G+EPLQT+AEIG+DGKL VVVR+S+++ S + S     GGG   + +TPR SNLT
Sbjct: 181 VISLNGREPLQTDAEIGDDGKLHVVVRRSSAASSMISSFNKSHGGGLNSSMITPRASNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF-LGNA----SPRH---SNYGNLG----------- 300
             EIYS+QSSR PTPR SSFN +DF+ + NA    SPRH   ++YG  G           
Sbjct: 241 GVEIYSVQSSREPTPRASSFNQTDFYAMFNASKAPSPRHGYTNSYGGAGAGPGGDVYSLQ 300

Query: 301 ----------NFDEE------SGGFKGRAMNG---NVNGYPA-PASGGLFSPMTGPAVAA 360
                     NFDEE        G  GR+M+G   N N  P+ P    +F+  T  A   
Sbjct: 301 SSKGVTPRTSNFDEEVMKTAKKAGRGGRSMSGELYNNNSVPSYPPPNPMFTGSTSGASGV 360

Query: 361 AAKKRVHGGDGG--------KDLHMFVWSSSASPVSEGGLN--VFRGGDYDGAGAGGMNQ 420
             K+   GG GG        K+++MFVWSSSASPVSE      + RG   D +    ++ 
Sbjct: 361 KKKESGGGGSGGGVGVGGQNKEMNMFVWSSSASPVSEANAKNAMTRGSSTDVSTDPKVSI 420

Query: 421 KDFDEFGR-------DEFSFGNKSAV--NGGGHDGG--PVLSKLGSSSTAELHPKSGDDT 480
              D           +  S G K  V  +  G++GG  P + K GS           D  
Sbjct: 421 PPHDNLATKAMQNLIENMSPGRKGHVEMDQDGNNGGKSPYMGKKGSDVE--------DGG 480

Query: 481 AESKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPAIVARSI 540
              +   MPPASVMTRLILIMVWRKLIRNPNTYSSL GLAWSL+SF+WNI MP I++ SI
Sbjct: 481 PGPRKQQMPPASVMTRLILIMVWRKLIRNPNTYSSLFGLAWSLVSFKWNIKMPTIMSGSI 540

Query: 541 AILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIAVGLKGV 596
           +ILSDAGLGMAMFSLGLFMALQPKIIACG ++A FAMAVRF+TGPAV+AA SIA+G++G 
Sbjct: 541 SILSDAGLGMAMFSLGLFMALQPKIIACGKSVAGFAMAVRFLTGPAVIAATSIAIGIRGD 600

BLAST of Cp4.1LG19g02490 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 662.1 bits (1707), Expect = 4.1e-190
Identity = 394/661 (59.61%), Postives = 462/661 (69.89%), Query Frame = 0

Query: 1   MISVSDLYHVLTAVVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIS 60
           MIS  DLY VLTAV+PLYVAMILAYGSV+WWKIFSPDQCSGINRFVA+FAVPLLSFHFIS
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNNPFSMNFRFIAADTLQKLIVLAALAVWSHLSSKGSLEWAITLFSLSTLPNTLVMGIPL 120
           +NNP++MN RFIAADTLQK+I+L+ L +W++ +  GSLEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDSTGTLMVQIVVLQCIIWYTLMLFLFEYRGARLLIAEQFPDTAGEIVSFRVDSD 180
           L  MYG+ +G+LMVQIVVLQCIIWYTL+LFLFE+RGA++LI EQFP+TA  IVSF+V+SD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESD 180

Query: 181 ILSLDGKEPLQTEAEIGEDGKLRVVVRKSASSRSEVFSRRSHGGGGGGGASLTPRPSNLT 240
           ++SLDG + L+T+AEIG+DGKL V VRKS +SR               G ++TPRPSNLT
Sbjct: 181 VVSLDGHDFLETDAEIGDDGKLHVTVRKSNASRRSFC-----------GPNMTPRPSNLT 240

Query: 241 NAEIYSLQSSRNPTPRGSSFNHSDFF--LGNASPRHSNYG---------------NLGNF 300
            AEIYSL +    TPRGS+FNHSDF+  +G    R SN+G                  NF
Sbjct: 241 GAEIYSLST----TPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNF 300

Query: 301 DEE----SGGFKGRAMNGNVNGYPAPASGGLFSPMTGPAVAAAAKKRVH----------- 360
           +E     S    G    G    YPAP      +P       + A K V+           
Sbjct: 301 EENCAMASSPRFGYYPGGGAGSYPAP------NPEFSSTTTSTANKSVNKNPKDVNTNQQ 360

Query: 361 -----GGDG----GKDLHMFVWSSSASPVSE-GGLNVFRGG-DYDGAGAGGMNQKDF--- 420
                GG       K+LHMFVWSS+ SPVS+  GLNVF G  D D  G      K+    
Sbjct: 361 TTLPTGGKSNSHDAKELHMFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRML 420

Query: 421 -------------------DEFGRDEFSFGNKSAVNGGGHDGGPVLSKLGSSSTAELHPK 480
                              D  G  +FSF  K        D    L+KL  +STA L  K
Sbjct: 421 VPDQSHNGETKAVAHPASGDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSK 480

Query: 481 SGDDTAE-SKPTAMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLAWSLISFRWNIVMPA 540
           +G   AE S+   MPPASVMTRLILIMVWRKLIRNPNTYSSLIGL W+L++FRW++ MP 
Sbjct: 481 TGLGGAEASQRKNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPK 540

Query: 541 IVARSIAILSDAGLGMAMFSLGLFMALQPKIIACGNTIATFAMAVRFITGPAVMAAASIA 596
           I+ +SI+ILSDAGLGMAMFSLGLFMALQPK+IACGN++ATFAMAVRF+TGPAVMA A+IA
Sbjct: 541 IIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIA 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q67UL31.0e-20667.87Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ92.0e-20566.56Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Q0IQA54.4e-20568.57Probable auxin efflux carrier component 1d OS=Oryza sativa subsp. japonica OX=39... [more]
Q9C6B82.8e-20463.90Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV... [more]
P0C0X53.7e-20468.69Probable auxin efflux carrier component 1b OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
XP_023518356.10.0100.00probable auxin efflux carrier component 1b [Cucurbita pepo subsp. pepo][more]
XP_022966088.10.099.33probable auxin efflux carrier component 1b [Cucurbita maxima][more]
XP_022931834.10.099.16probable auxin efflux carrier component 1b [Cucurbita moschata][more]
XP_038880940.10.093.07probable auxin efflux carrier component 1b isoform X2 [Benincasa hispida][more]
XP_008455726.10.093.52PREDICTED: probable auxin efflux carrier component 1b [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1HQV80.099.33Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111465879 PE=3 ... [more]
A0A6J1EVC10.099.16Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111438127 PE=... [more]
A0A1S3C1I40.093.52Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103495829 PE=3 SV=1[more]
A0A0A0KYF40.093.19Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=Csa_4G430820 PE=3 S... [more]
D7URM40.093.19Auxin efflux carrier component OS=Cucumis sativus OX=3659 GN=CsPIN4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73590.12.0e-20563.90Auxin efflux carrier family protein [more]
AT1G23080.16.7e-19361.97Auxin efflux carrier family protein [more]
AT1G23080.38.8e-19361.66Auxin efflux carrier family protein [more]
AT5G57090.12.2e-19160.00Auxin efflux carrier family protein [more]
AT1G70940.14.1e-19059.61Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..595
e-value: 8.1E-160
score: 529.4
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 9..590
e-value: 3.9E-188
score: 624.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 215..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 388..428
NoneNo IPR availablePANTHERPTHR31752:SF43AUXIN EFFLUX CARRIER COMPONENTcoord: 1..595
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..595

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g02490.1Cp4.1LG19g02490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity