Cp4.1LG19g01020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG19g01020 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 3.0e-193
Identity = 332/611 (54.34%), Postives = 430/611 (70.38%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQK 123
           V  DI   +   G +   +CSF E                            M + FN +
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHE----------------------------MAEEFNVR 132

Query: 124 DGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRS 183
            G+    P G FN  F +TGS Q+DAA+ K+L+  G++IPL   +L   +L+LH  ++R+
Sbjct: 133 SGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRA 192

Query: 184 VPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRF 243
           VP+SWDP +LASFIEN+GTH++TS+TIGG+DV+Y++QHQSSPL   E++ YV D+   RF
Sbjct: 193 VPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRF 252

Query: 244 SDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQN 303
            + + Q+ +G  K K+K                          D+TVIFRRRGGDDLEQ+
Sbjct: 253 HEAESQSITGPLKYKDK--------------------------DITVIFRRRGGDDLEQS 312

Query: 304 HVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQI 363
           H +WA+TV ++PD+I MTF PI +LL+GV G  HLTRAI LYLEYKPP+E+L+YFL++QI
Sbjct: 313 HARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQI 372

Query: 364 PRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQN 423
            R WAP +  +   QRKEPVC SLQFS+MG KL++S +Q+ +GRKPVTG+ LSLEG KQN
Sbjct: 373 ARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQN 432

Query: 424 RLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIEN 483
           RLSIHLQHL +LPKILQP+WD+H+ IGAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+
Sbjct: 433 RLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEH 492

Query: 484 PETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKL 543
            ET +G+LSGV+IVTGAQLGVW FGS+ VL++KLL+S++PGCTIRR++WDH P   S +L
Sbjct: 493 TETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRL 552

Query: 544 VSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRI 603
                +++++ ++ S      Q  KLAK+VD++EM KGPQD PGHWLVTGAKLGVEK +I
Sbjct: 553 EPGGPSTSSSTEEVSG-----QSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKI 561

Query: 604 VLRVKYSLLNY 614
           VLRVKYSLLNY
Sbjct: 613 VLRVKYSLLNY 561

BLAST of Cp4.1LG19g01020 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 4.9e-111
Identity = 248/627 (39.55%), Postives = 362/627 (57.74%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS  IK   
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIK--- 73

Query: 74  QPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGYPS 133
                     C   E + +                + I+ + QM + FNQ+  L+   PS
Sbjct: 74  ----------CDKGERMRF---------------RSDILPFQQMAEQFNQELSLAGKIPS 133

Query: 134 GSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPA 193
           G FN  F F+   Q DAA  KNL+ DG +I L    L  + +LL E+VK++VP++WDP A
Sbjct: 134 GLFNAMFEFSSCWQKDAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAA 193

Query: 194 LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGS 253
           LA FI+ +GTH+I S+ +GGKDVIY KQ  SS L   ++++ ++++A++RF +      +
Sbjct: 194 LARFIDIYGTHIIVSVKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNT 253

Query: 254 GSRKL--------KEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQ-N 313
           GS ++        KE+ L     ++ G Y         + KED   + +RRGG+D     
Sbjct: 254 GSERVQASSKVETKEQRLRFADTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLM 313

Query: 314 HVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQI 373
           H +W +TV+  PDVI M+F PIT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+
Sbjct: 314 HNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQL 373

Query: 374 PRVWAPVRDTIP-GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQ 433
           PR WAPV   +P G QRK+  C SLQFS  G KLYV+   + +G++P+TGM L LEG + 
Sbjct: 374 PRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRS 433

Query: 434 NRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIE 493
           NRL+IHLQHL++LPKI Q   D + +I        E  D R++E V WKN+SHV T P+E
Sbjct: 434 NRLAIHLQHLSSLPKIYQLEDDLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVE 493

Query: 494 NPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCT-IRRTLWDHLPN--DK 553
           + +    +LS   +VTGAQL V   G + VL+++L +SR+ G T ++ + WD       K
Sbjct: 494 SDD----DLS---VVTGAQLHVESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPK 553

Query: 554 SKKLVSSSDNSNTAADDSSSGSKNI---------------QGNKLAKLVDTTEMSKGPQD 611
           S  + +   +  TAA        ++               Q  KL K VDT+EM++GPQ+
Sbjct: 554 SGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQE 590

BLAST of Cp4.1LG19g01020 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.9e-100
Identity = 240/631 (38.03%), Postives = 330/631 (52.30%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGY 130
            +       S I SF+                            QM + FNQ   LS   
Sbjct: 75  DKGERTRLRSDILSFN----------------------------QMSEKFNQDMCLSGKI 134

Query: 131 PSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDP 190
           PSG FN  F F+     DA++ K L+ DG++I L   ++    L L + VKR VP+SWD 
Sbjct: 135 PSGMFNNMFAFSKCWPKDASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDS 194

Query: 191 PALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS----DT 250
            ALA FIE +GTHV+  +T+GGKDVI+VKQ + S     E+++ ++   ++RF     ++
Sbjct: 195 AALAGFIEKYGTHVVVGVTMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVES 254

Query: 251 QGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAP----YPSGKEDVTVIFRRRGGDDLEQ 310
           +  A   S K KE+ L        G+ P  TS        +  E++  +  RRGG DL Q
Sbjct: 255 KSPASVYSGKPKEENL-----LQWGLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQ 314

Query: 311 NHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQ 370
           +H +W  TV  +P+VI M F PIT+LL G+ G   L+ A+ LYL YKPP+EEL  FLEFQ
Sbjct: 315 SHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQ 374

Query: 371 IPRVWAPVRDTIP-GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 430
           +PR WAPV   +P G +R +   PSLQFS+MG KLYV+  ++  G +PVTG+   LEG K
Sbjct: 375 LPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKK 434

Query: 431 QNRLSIHLQHLATLPKILQ-PYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAP 490
            N L+IHLQHL+  P  L   + DT+  I  P  +G       ++ PVKW  FSHV T P
Sbjct: 435 GNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG-------YYVPVKWGIFSHVCTYP 494

Query: 491 IENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKS 550
           ++       + +   IVT A L V G G R VL+++L +S       R++ WD+L  +  
Sbjct: 495 VQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSR 554

Query: 551 KKLVSS----------SDNSNTAADDSS---------SGSKNIQGNKLAKLVDTTEMSKG 610
           K  V S          S N  T    S           G       KL  LVDT E+ +G
Sbjct: 555 KSGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPVKPKLLSLVDTKEVMRG 603

Query: 611 PQDSPGHWLVTGAKLGVEKSRIVLRVKYSLL 612
           P++ PG+W+VTGAKL VE  +I ++ KYSLL
Sbjct: 615 PEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Cp4.1LG19g01020 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 8.8e-92
Identity = 223/650 (34.31%), Postives = 333/650 (51.23%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVEVDDEHTRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADIN- 71

Query: 74  FQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGY 133
                       C   E   +                + I+ + +M +YFNQ+  ++   
Sbjct: 72  ------------CDKGERTRF---------------RSDILDFNKMSEYFNQRSSVTGKI 131

Query: 134 PSGSFNTAFGF-TGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTS-LLLHENVKRSVPTSW 193
           PSG+FN  FGF +GS   DAA  K+L  D   + L    + + + L L + V+ +VP+SW
Sbjct: 132 PSGNFNATFGFQSGSWATDAANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSW 191

Query: 194 DPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG 253
           DP  LA FIE +GTHVIT +++GG+DV+ V+Q +SS L    ++ ++ D+ +Q F     
Sbjct: 192 DPQLLARFIERYGTHVITGVSVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLF----- 251

Query: 254 QAGSGSRKLKEKGLDGGLFNNQ---------GVYPQPTSAPYP----SGKEDVTVIFRRR 313
              +GS  L  + L+    ++           V+    +  +     + +  +TVI  +R
Sbjct: 252 ---TGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDDKQTVAFNNFSINSQNGITVICAKR 311

Query: 314 GGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEEL 373
           GGD   ++H +W  TV   PD I   F PIT+LL  V G   L+ A+ LYL YKPPL +L
Sbjct: 312 GGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDL 371

Query: 374 RYFLEFQIPRVWAPVRDTIP--GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGM 433
           +YFL+F  PR WAPV + +P           P+L  + MG KLYV+   +   + PVTGM
Sbjct: 372 QYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGM 431

Query: 434 CLSLEGGKQNRLSIHLQHL-ATLPKILQPYWDTHIAIGAPKWQGPEE--QDSRWFEPVKW 493
              LEG K NRL+IHLQHL  T   + +   D HI      W+G ++   + R+FEP+  
Sbjct: 432 RFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI------WRGSDQITDNDRYFEPLNG 491

Query: 494 KNFSHVSTAPIENPETFMGELSG-------VYIVTGAQLGVWGFGSRYVLYMKLLYSRLP 553
           K FSHV T P++    ++   S         +IVTGAQL V   GS+ VL+++L Y+++ 
Sbjct: 492 KKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVS 551

Query: 554 GCTIRRTLWDHLPNDKSKK----------LVSSSDNSNTAADDSS----------SGSKN 613
              + +  W H P   S+K          L S S + N    D +           G   
Sbjct: 552 DHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPV 611

BLAST of Cp4.1LG19g01020 vs. NCBI nr
Match: KAG7027584.1 (MACPF domain-containing protein CAD1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1236 bits (3199), Expect = 0.0
Identity = 609/613 (99.35%), Postives = 609/613 (99.35%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIA EL NALIIIYLQMVDYFN
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIASELTNALIIIYLQMVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN AADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNAAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 613

BLAST of Cp4.1LG19g01020 vs. NCBI nr
Match: XP_023517935.1 (MACPF domain-containing protein CAD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1179 bits (3051), Expect = 0.0
Identity = 585/613 (95.43%), Postives = 585/613 (95.43%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQQPIGHRSSGICSFDE                            MVDYFN
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDE----------------------------MVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 585

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 585

BLAST of Cp4.1LG19g01020 vs. NCBI nr
Match: XP_022925277.1 (MACPF domain-containing protein CAD1-like [Cucurbita moschata])

HSP 1 Score: 1174 bits (3037), Expect = 0.0
Identity = 583/613 (95.11%), Postives = 583/613 (95.11%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQQPIGHRSSGICSFDE                            MVDYFN
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDE----------------------------MVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN AADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNAAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 585

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 585

BLAST of Cp4.1LG19g01020 vs. NCBI nr
Match: XP_022966250.1 (MACPF domain-containing protein CAD1-like [Cucurbita maxima])

HSP 1 Score: 1160 bits (3002), Expect = 0.0
Identity = 577/613 (94.13%), Postives = 579/613 (94.45%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKN QQPIGHRSSGICSFDE                            MVDYFN
Sbjct: 61  LNVSWDIKNSQQPIGHRSSGICSFDE----------------------------MVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDA AAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDADAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPIT+LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITSLLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHL+HLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLKHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN AADDSSSGSKN QGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNYAADDSSSGSKNTQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 585

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 585

BLAST of Cp4.1LG19g01020 vs. NCBI nr
Match: XP_022144069.1 (MACPF domain-containing protein CAD1-like [Momordica charantia])

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 534/613 (87.11%), Postives = 555/613 (90.54%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQ+PIGHRSSGICSFDE                            MV+YFN
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDE----------------------------MVNYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGY SGSFN+AFGF GSKQIDAA  KNLS DGFYIPLAKFQ T T L+L ENVK
Sbjct: 121 QKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           R++PTSWDPP+LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQG  GSGS KLKEKGLDGGLFNNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGLTGSGSIKLKEKGLDGGLFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHV+WA+TVR SPDVIEMTF+PITALLDGV GKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVRWARTVRLSPDVIEMTFFPITALLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDT+PGHQRKEPVCPSLQFSIMGQKLYVSIEQI IGRKPVTGM LSLEG K
Sbjct: 361 QIPRVWAPVRDTVPGHQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLA+LPKILQPYWDTH+ IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLASLPKILQPYWDTHVGIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVW FG R VLYMKLLYSRLPGCTI+RTLWDHL NDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWDFGPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN  ADDSSSGS+NIQGNKLAKLVDT+EMSKGP D PGHWLVTGAKLGVEK 
Sbjct: 541 KLVSSSDNSN--ADDSSSGSRNIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKG 583

Query: 601 RIVLRVKYSLLNY 613
           +IVLRVKYSLLNY
Sbjct: 601 KIVLRVKYSLLNY 583

BLAST of Cp4.1LG19g01020 vs. ExPASy TrEMBL
Match: A0A6J1EBB6 (MACPF domain-containing protein CAD1-like OS=Cucurbita moschata OX=3662 GN=LOC111432562 PE=4 SV=1)

HSP 1 Score: 1174 bits (3037), Expect = 0.0
Identity = 583/613 (95.11%), Postives = 583/613 (95.11%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQQPIGHRSSGICSFDE                            MVDYFN
Sbjct: 61  LNVSWDIKNFQQPIGHRSSGICSFDE----------------------------MVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN AADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNAAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 585

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 585

BLAST of Cp4.1LG19g01020 vs. ExPASy TrEMBL
Match: A0A6J1HR44 (MACPF domain-containing protein CAD1-like OS=Cucurbita maxima OX=3661 GN=LOC111465969 PE=4 SV=1)

HSP 1 Score: 1160 bits (3002), Expect = 0.0
Identity = 577/613 (94.13%), Postives = 579/613 (94.45%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKN QQPIGHRSSGICSFDE                            MVDYFN
Sbjct: 61  LNVSWDIKNSQQPIGHRSSGICSFDE----------------------------MVDYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFNTAFGFTGSKQIDA AAKNLSTDGFYIPLAKFQLTSTSLLLHENVK
Sbjct: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDADAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHVKWAKTVRSSPDVIEMTFYPIT+LLDGVAGKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVKWAKTVRSSPDVIEMTFYPITSLLDGVAGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK
Sbjct: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHL+HLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLKHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVWGFGSR VLYMKLLYSRLPGCTIRRTLWDHLPNDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN AADDSSSGSKN QGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS
Sbjct: 541 KLVSSSDNSNYAADDSSSGSKNTQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 585

Query: 601 RIVLRVKYSLLNY 613
           RIVLRVKYSLLNY
Sbjct: 601 RIVLRVKYSLLNY 585

BLAST of Cp4.1LG19g01020 vs. ExPASy TrEMBL
Match: A0A6J1CR77 (MACPF domain-containing protein CAD1-like OS=Momordica charantia OX=3673 GN=LOC111013848 PE=4 SV=1)

HSP 1 Score: 1079 bits (2791), Expect = 0.0
Identity = 534/613 (87.11%), Postives = 555/613 (90.54%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ++GTALHTAINAVQALGRGFDVNFDTRLLYCKGV+GSTIVEVDDEHTRDICLYDDVVL
Sbjct: 1   MGDTGTALHTAINAVQALGRGFDVNFDTRLLYCKGVSGSTIVEVDDEHTRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
           LNVSWDIKNFQ+PIGHRSSGICSFDE                            MV+YFN
Sbjct: 61  LNVSWDIKNFQEPIGHRSSGICSFDE----------------------------MVNYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGY SGSFN+AFGF GSKQIDAA  KNLS DGFYIPLAKFQ T T L+L ENVK
Sbjct: 121 QKDGLSEGYTSGSFNSAFGFNGSKQIDAATTKNLSMDGFYIPLAKFQFTKTPLVLQENVK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           R++PTSWDPP+LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RAIPTSWDPPSLASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQG  GSGS KLKEKGLDGGLFNNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGLTGSGSIKLKEKGLDGGLFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHV+WA+TVR SPDVIEMTF+PITALLDGV GKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVRWARTVRLSPDVIEMTFFPITALLDGVTGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAPVRDT+PGHQRKEPVCPSLQFSIMGQKLYVSIEQI IGRKPVTGM LSLEG K
Sbjct: 361 QIPRVWAPVRDTVPGHQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLA+LPKILQPYWDTH+ IGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLASLPKILQPYWDTHVGIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSK 540
           ENPETFMGELSGVYIVTGAQLGVW FG R VLYMKLLYSRLPGCTI+RTLWDHL NDKSK
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWDFGPRNVLYMKLLYSRLPGCTIQRTLWDHLLNDKSK 540

Query: 541 KLVSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKS 600
           KLVSSSDNSN  ADDSSSGS+NIQGNKLAKLVDT+EMSKGP D PGHWLVTGAKLGVEK 
Sbjct: 541 KLVSSSDNSN--ADDSSSGSRNIQGNKLAKLVDTSEMSKGPHDPPGHWLVTGAKLGVEKG 583

Query: 601 RIVLRVKYSLLNY 613
           +IVLRVKYSLLNY
Sbjct: 601 KIVLRVKYSLLNY 583

BLAST of Cp4.1LG19g01020 vs. ExPASy TrEMBL
Match: A0A5D3CHB3 (MACPF domain-containing protein CAD1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold127G00610 PE=4 SV=1)

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 530/615 (86.18%), Postives = 554/615 (90.08%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
            NVSWDIKNFQ+PIGHRSSGICSFDE                            M DYFN
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDE----------------------------MADYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFN+AFGFTG +Q DA+A K+LSTDGFYIPLAKFQ T T L L EN+K
Sbjct: 121 QKDGLSEGYPSGSFNSAFGFTGLRQTDASATKSLSTDGFYIPLAKFQFTKTPLELKENIK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           R+VPT+WDPP+LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQ GSGS KLKEKGLDGGLFNNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHV+WA+TVRSSPDVIEMTFYPITALLDGV GKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVRWARTVRSSPDVIEMTFYPITALLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAP+ D +PG QRKEPVCPSLQFSIMGQKLYVSIEQI IGRKPVTGM LSLEG K
Sbjct: 361 QIPRVWAPIHDKLPGRQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLA+LPKI+ PYWDTH+AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLASLPKIMHPYWDTHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPN-DKS 540
           ENPETFMGELSGVYIVTGAQLGVW FGSR VLYMKLLYSRLPGCTIRRTLWDHLP  +KS
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKS 540

Query: 541 KKLVSSSDNSNTAADDSSSGSKNIQGN-KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE 600
           KKLVSSSDN+N A D+SSSG+K+ QGN KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE
Sbjct: 541 KKLVSSSDNTNNA-DNSSSGTKSTQGNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE 586

Query: 601 KSRIVLRVKYSLLNY 613
           K +IVLRVKYSLLNY
Sbjct: 601 KGKIVLRVKYSLLNY 586

BLAST of Cp4.1LG19g01020 vs. ExPASy TrEMBL
Match: A0A1S3CJ73 (MACPF domain-containing protein CAD1-like OS=Cucumis melo OX=3656 GN=LOC103501463 PE=4 SV=1)

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 530/615 (86.18%), Postives = 554/615 (90.08%), Query Frame = 0

Query: 1   MEESGTALHTAINAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVL 60
           M ESGTALHTA NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEH+RDICLYDDVVL
Sbjct: 1   MGESGTALHTASNAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHSRDICLYDDVVL 60

Query: 61  LNVSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFN 120
            NVSWDIKNFQ+PIGHRSSGICSFDE                            M DYFN
Sbjct: 61  HNVSWDIKNFQEPIGHRSSGICSFDE----------------------------MADYFN 120

Query: 121 QKDGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVK 180
           QKDGLSEGYPSGSFN+AFGFTG +Q DA+A K+LSTDGFYIPLAKFQ T T L L EN+K
Sbjct: 121 QKDGLSEGYPSGSFNSAFGFTGLRQTDASATKSLSTDGFYIPLAKFQFTKTPLELKENIK 180

Query: 181 RSVPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240
           R+VPT+WDPP+LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ
Sbjct: 181 RAVPTTWDPPSLASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQ 240

Query: 241 RFSDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLE 300
           RFSDTQGQ GSGS KLKEKGLDGGLFNNQGVYPQPTSAPY +GKEDVTVIFRRRGGDDLE
Sbjct: 241 RFSDTQGQTGSGSIKLKEKGLDGGLFNNQGVYPQPTSAPYLTGKEDVTVIFRRRGGDDLE 300

Query: 301 QNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEF 360
           QNHV+WA+TVRSSPDVIEMTFYPITALLDGV GKEHLTRAI LYLEYKPPLEELRYFLEF
Sbjct: 301 QNHVRWARTVRSSPDVIEMTFYPITALLDGVVGKEHLTRAILLYLEYKPPLEELRYFLEF 360

Query: 361 QIPRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 420
           QIPRVWAP+ D +PG QRKEPVCPSLQFSIMGQKLYVSIEQI IGRKPVTGM LSLEG K
Sbjct: 361 QIPRVWAPIHDKLPGRQRKEPVCPSLQFSIMGQKLYVSIEQISIGRKPVTGMRLSLEGSK 420

Query: 421 QNRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480
           QNRLSIHLQHLA+LPKI+ PYWDTH+AIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI
Sbjct: 421 QNRLSIHLQHLASLPKIMHPYWDTHVAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPI 480

Query: 481 ENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPN-DKS 540
           ENPETFMGELSGVYIVTGAQLGVW FGSR VLYMKLLYSRLPGCTIRRTLWDHLP  +KS
Sbjct: 481 ENPETFMGELSGVYIVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRTLWDHLPPIEKS 540

Query: 541 KKLVSSSDNSNTAADDSSSGSKNIQGN-KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE 600
           KKLVSSSDN+N A D+SSSG+K+ QGN KLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE
Sbjct: 541 KKLVSSSDNTNNA-DNSSSGTKSTQGNNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVE 586

Query: 601 KSRIVLRVKYSLLNY 613
           K +IVLRVKYSLLNY
Sbjct: 601 KGKIVLRVKYSLLNY 586

BLAST of Cp4.1LG19g01020 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 676.4 bits (1744), Expect = 2.2e-194
Identity = 332/611 (54.34%), Postives = 430/611 (70.38%), Query Frame = 0

Query: 4   SGTALHTAI-NAVQALGRGFDVNFDTRLLYCKGVAGSTIVEVDDEHTRDICLYDDVVLLN 63
           S  AL T + NA+QALGRGFDV  D RLLYCKG  GS +V +++   RD+ L    +L N
Sbjct: 13  SSEALTTTLRNAIQALGRGFDVTSDVRLLYCKGAPGSRLVRIEEGQNRDLELSHGFLLPN 72

Query: 64  VSWDIKNFQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQK 123
           V  DI   +   G +   +CSF E                            M + FN +
Sbjct: 73  VPADIDCSRGNSGTQRISVCSFHE----------------------------MAEEFNVR 132

Query: 124 DGLSEGYPSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRS 183
            G+    P G FN  F +TGS Q+DAA+ K+L+  G++IPL   +L   +L+LH  ++R+
Sbjct: 133 SGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYFIPLYDVKLAKLTLVLHNEIRRA 192

Query: 184 VPTSWDPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRF 243
           VP+SWDP +LASFIEN+GTH++TS+TIGG+DV+Y++QHQSSPL   E++ YV D+   RF
Sbjct: 193 VPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQHQSSPLPVSEIENYVNDMIKHRF 252

Query: 244 SDTQGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQN 303
            + + Q+ +G  K K+K                          D+TVIFRRRGGDDLEQ+
Sbjct: 253 HEAESQSITGPLKYKDK--------------------------DITVIFRRRGGDDLEQS 312

Query: 304 HVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQI 363
           H +WA+TV ++PD+I MTF PI +LL+GV G  HLTRAI LYLEYKPP+E+L+YFL++QI
Sbjct: 313 HARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAIELYLEYKPPIEDLQYFLDYQI 372

Query: 364 PRVWAPVRDTIPGHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQN 423
            R WAP +  +   QRKEPVC SLQFS+MG KL++S +Q+ +GRKPVTG+ LSLEG KQN
Sbjct: 373 ARAWAPEQSNL---QRKEPVCSSLQFSLMGPKLFISADQVTVGRKPVTGLRLSLEGSKQN 432

Query: 424 RLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIEN 483
           RLSIHLQHL +LPKILQP+WD+H+ IGAPKWQGPEEQDSRWFEP+KWKNFSHVST+PIE+
Sbjct: 433 RLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQDSRWFEPIKWKNFSHVSTSPIEH 492

Query: 484 PETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKSKKL 543
            ET +G+LSGV+IVTGAQLGVW FGS+ VL++KLL+S++PGCTIRR++WDH P   S +L
Sbjct: 493 TETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSKVPGCTIRRSVWDHTPVASSGRL 552

Query: 544 VSSSDNSNTAADDSSSGSKNIQGNKLAKLVDTTEMSKGPQDSPGHWLVTGAKLGVEKSRI 603
                +++++ ++ S      Q  KLAK+VD++EM KGPQD PGHWLVTGAKLGVEK +I
Sbjct: 553 EPGGPSTSSSTEEVSG-----QSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKI 561

Query: 604 VLRVKYSLLNY 614
           VLRVKYSLLNY
Sbjct: 613 VLRVKYSLLNY 561

BLAST of Cp4.1LG19g01020 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 403.3 bits (1035), Expect = 3.5e-112
Identity = 248/627 (39.55%), Postives = 362/627 (57.74%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS  IK   
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIK--- 73

Query: 74  QPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGYPS 133
                     C   E + +                + I+ + QM + FNQ+  L+   PS
Sbjct: 74  ----------CDKGERMRF---------------RSDILPFQQMAEQFNQELSLAGKIPS 133

Query: 134 GSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPA 193
           G FN  F F+   Q DAA  KNL+ DG +I L    L  + +LL E+VK++VP++WDP A
Sbjct: 134 GLFNAMFEFSSCWQKDAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAA 193

Query: 194 LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGS 253
           LA FI+ +GTH+I S+ +GGKDVIY KQ  SS L   ++++ ++++A++RF +      +
Sbjct: 194 LARFIDIYGTHIIVSVKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNT 253

Query: 254 GSRKL--------KEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQ-N 313
           GS ++        KE+ L     ++ G Y         + KED   + +RRGG+D     
Sbjct: 254 GSERVQASSKVETKEQRLRFADTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLM 313

Query: 314 HVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQI 373
           H +W +TV+  PDVI M+F PIT+LL+GV G   L+ AI LYL YKPP+EEL  FLEFQ+
Sbjct: 314 HNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQL 373

Query: 374 PRVWAPVRDTIP-GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGKQ 433
           PR WAPV   +P G QRK+  C SLQFS  G KLYV+   + +G++P+TGM L LEG + 
Sbjct: 374 PRQWAPVFSELPLGPQRKQQSCASLQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRS 433

Query: 434 NRLSIHLQHLATLPKILQPYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAPIE 493
           NRL+IHLQHL++LPKI Q   D + +I        E  D R++E V WKN+SHV T P+E
Sbjct: 434 NRLAIHLQHLSSLPKIYQLEDDLNRSI------RQESHDRRYYEKVNWKNYSHVCTEPVE 493

Query: 494 NPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCT-IRRTLWDHLPN--DK 553
           + +    +LS   +VTGAQL V   G + VL+++L +SR+ G T ++ + WD       K
Sbjct: 494 SDD----DLS---VVTGAQLHVESHGFKNVLFLRLCFSRVVGATLVKNSEWDEAVGFAPK 553

Query: 554 SKKLVSSSDNSNTAADDSSSGSKNI---------------QGNKLAKLVDTTEMSKGPQD 611
           S  + +   +  TAA        ++               Q  KL K VDT+EM++GPQ+
Sbjct: 554 SGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPPVPTQAPKLLKFVDTSEMTRGPQE 590

BLAST of Cp4.1LG19g01020 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 367.1 bits (941), Expect = 2.8e-101
Identity = 240/631 (38.03%), Postives = 330/631 (52.30%), Query Frame = 0

Query: 11  AINAVQALGRGFDVNFDTRLLYCKGVA-GSTIVEVDDEHTRDICLYDDVVLLNVSWDIKN 70
           A  AV  +G G+D+  D R   CK    GS +VE+D    RD+     +V+ NVS  IK 
Sbjct: 15  AEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNNVSSSIKC 74

Query: 71  FQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGY 130
            +       S I SF+                            QM + FNQ   LS   
Sbjct: 75  DKGERTRLRSDILSFN----------------------------QMSEKFNQDMCLSGKI 134

Query: 131 PSGSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDP 190
           PSG FN  F F+     DA++ K L+ DG++I L   ++    L L + VKR VP+SWD 
Sbjct: 135 PSGMFNNMFAFSKCWPKDASSVKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDS 194

Query: 191 PALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFS----DT 250
            ALA FIE +GTHV+  +T+GGKDVI+VKQ + S     E+++ ++   ++RF     ++
Sbjct: 195 AALAGFIEKYGTHVVVGVTMGGKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVES 254

Query: 251 QGQAGSGSRKLKEKGLDGGLFNNQGVYPQPTSAP----YPSGKEDVTVIFRRRGGDDLEQ 310
           +  A   S K KE+ L        G+ P  TS        +  E++  +  RRGG DL Q
Sbjct: 255 KSPASVYSGKPKEENL-----LQWGLQPFGTSVSSAVVMHTKNEEIMRVCIRRGGVDLGQ 314

Query: 311 NHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEELRYFLEFQ 370
           +H +W  TV  +P+VI M F PIT+LL G+ G   L+ A+ LYL YKPP+EEL  FLEFQ
Sbjct: 315 SHERWLSTVSQAPNVISMCFVPITSLLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQ 374

Query: 371 IPRVWAPVRDTIP-GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGMCLSLEGGK 430
           +PR WAPV   +P G +R +   PSLQFS+MG KLYV+  ++  G +PVTG+   LEG K
Sbjct: 375 LPRQWAPVYGDLPLGLRRSKQSSPSLQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKK 434

Query: 431 QNRLSIHLQHLATLPKILQ-PYWDTHIAIGAPKWQGPEEQDSRWFEPVKWKNFSHVSTAP 490
            N L+IHLQHL+  P  L   + DT+  I  P  +G       ++ PVKW  FSHV T P
Sbjct: 435 GNHLAIHLQHLSACPPSLHLSHDDTYEPIEEPVEKG-------YYVPVKWGIFSHVCTYP 494

Query: 491 IENPETFMGELSGVYIVTGAQLGVWGFGSRYVLYMKLLYSRLPGCTIRRTLWDHLPNDKS 550
           ++       + +   IVT A L V G G R VL+++L +S       R++ WD+L  +  
Sbjct: 495 VQYNGARSDDTAS--IVTKAWLEVKGMGMRKVLFLRLGFSLDASAVTRKSCWDNLSTNSR 554

Query: 551 KKLVSS----------SDNSNTAADDSS---------SGSKNIQGNKLAKLVDTTEMSKG 610
           K  V S          S N  T    S           G       KL  LVDT E+ +G
Sbjct: 555 KSGVFSMISTRLSTGLSPNPATTKPQSKIDINSAVYPRGPSPPVKPKLLSLVDTKEVMRG 603

Query: 611 PQDSPGHWLVTGAKLGVEKSRIVLRVKYSLL 612
           P++ PG+W+VTGAKL VE  +I ++ KYSLL
Sbjct: 615 PEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 603

BLAST of Cp4.1LG19g01020 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 339.3 bits (869), Expect = 6.2e-93
Identity = 223/650 (34.31%), Postives = 333/650 (51.23%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVAGST----IVEVDDEHTRDICLYDDVVLLNVSWDIKN 73
           AV++LG+GFD+  D RL YCK   GS     +V +D    R++ +    V  NVS DI  
Sbjct: 12  AVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADIN- 71

Query: 74  FQQPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGY 133
                       C   E   +                + I+ + +M +YFNQ+  ++   
Sbjct: 72  ------------CDKGERTRF---------------RSDILDFNKMSEYFNQRSSVTGKI 131

Query: 134 PSGSFNTAFGF-TGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTS-LLLHENVKRSVPTSW 193
           PSG+FN  FGF +GS   DAA  K+L  D   + L    + + + L L + V+ +VP+SW
Sbjct: 132 PSGNFNATFGFQSGSWATDAANVKSLGLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSW 191

Query: 194 DPPALASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQG 253
           DP  LA FIE +GTHVIT +++GG+DV+ V+Q +SS L    ++ ++ D+ +Q F     
Sbjct: 192 DPQLLARFIERYGTHVITGVSVGGQDVVVVRQDKSSDLDNDLLRHHLYDLGDQLF----- 251

Query: 254 QAGSGSRKLKEKGLDGGLFNNQ---------GVYPQPTSAPYP----SGKEDVTVIFRRR 313
              +GS  L  + L+    ++           V+    +  +     + +  +TVI  +R
Sbjct: 252 ---TGSCLLSTRRLNKAYHHSHSQPKFPEAFNVFDDKQTVAFNNFSINSQNGITVICAKR 311

Query: 314 GGDDLEQNHVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKPPLEEL 373
           GGD   ++H +W  TV   PD I   F PIT+LL  V G   L+ A+ LYL YKPPL +L
Sbjct: 312 GGDGRAKSHSEWLITVPDKPDAINFNFIPITSLLKDVPGSGLLSHAMSLYLRYKPPLMDL 371

Query: 374 RYFLEFQIPRVWAPVRDTIP--GHQRKEPVCPSLQFSIMGQKLYVSIEQILIGRKPVTGM 433
           +YFL+F  PR WAPV + +P           P+L  + MG KLYV+   +   + PVTGM
Sbjct: 372 QYFLDFSGPRAWAPVHNDLPFGAAPNMASAYPALHINFMGPKLYVNTTPVTSEKNPVTGM 431

Query: 434 CLSLEGGKQNRLSIHLQHL-ATLPKILQPYWDTHIAIGAPKWQGPEE--QDSRWFEPVKW 493
              LEG K NRL+IHLQHL  T   + +   D HI      W+G ++   + R+FEP+  
Sbjct: 432 RFFLEGKKCNRLAIHLQHLDNTRTTVGEKITDEHI------WRGSDQITDNDRYFEPLNG 491

Query: 494 KNFSHVSTAPIENPETFMGELSG-------VYIVTGAQLGVWGFGSRYVLYMKLLYSRLP 553
           K FSHV T P++    ++   S         +IVTGAQL V   GS+ VL+++L Y+++ 
Sbjct: 492 KKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIVTGAQLEVKKHGSKSVLHLRLRYTKVS 551

Query: 554 GCTIRRTLWDHLPNDKSKK----------LVSSSDNSNTAADDSS----------SGSKN 613
              + +  W H P   S+K          L S S + N    D +           G   
Sbjct: 552 DHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGSVHHNMIQKDKNEVVLDSGVFPGGPPV 611

BLAST of Cp4.1LG19g01020 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 195.3 bits (495), Expect = 1.5e-49
Identity = 127/359 (35.38%), Postives = 195/359 (54.32%), Query Frame = 0

Query: 14  AVQALGRGFDVNFDTRLLYCKGVA-GSTIVEV-DDEHTRDICLYDDVVLLNVSWDIKNFQ 73
           A+ ++G G+D+  D RL YCKG +  S ++++ + +   +I L   + + NVS  IK   
Sbjct: 14  AIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGISIPNVSKSIK--- 73

Query: 74  QPIGHRSSGICSFDEIVTYGLSLENPVAIAGELINALIIIYLQMVDYFNQKDGLSEGYPS 133
                     C   E + +                + I+ + QM + FNQ+  L+   PS
Sbjct: 74  ----------CDKGERMRF---------------RSDILPFQQMAEQFNQELSLAGKIPS 133

Query: 134 GSFNTAFGFTGSKQIDAAAAKNLSTDGFYIPLAKFQLTSTSLLLHENVKRSVPTSWDPPA 193
           G FN  F F+   Q DAA  KNL+ DG +I L    L  + +LL E+VK++VP++WDP A
Sbjct: 134 GLFNAMFEFSSCWQKDAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAA 193

Query: 194 LASFIENFGTHVITSITIGGKDVIYVKQHQSSPLSTMEVKRYVQDIANQRFSDTQGQAGS 253
           LA FI+ +GTH+I S+ +GGKDVIY KQ  SS L   ++++ ++++A++RF +      +
Sbjct: 194 LARFIDIYGTHIIVSVKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNT 253

Query: 254 GSRKL--------KEKGLDGGLFNNQGVYPQPTSAPYPSGKEDVTVIFRRRGGDDLEQ-N 313
           GS ++        KE+ L     ++ G Y         + KED   + +RRGG+D     
Sbjct: 254 GSERVQASSKVETKEQRLRFADTSSLGSY---------ANKEDYVFMCKRRGGNDNRNLM 313

Query: 314 HVKWAKTVRSSPDVIEMTFYPITALLDGVAGKEHLTRAIRLYLEYKP--PLEELRYFLE 360
           H +W +TV+  PDVI M+F PIT+LL+GV G   L+ AI LYL  KP   L  + +FLE
Sbjct: 314 HNEWLQTVQMEPDVISMSFIPITSLLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335