Cp4.1LG19g00980 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g00980
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionvacuolar protein sorting-associated protein 20 homolog 2-like
LocationCp4.1LG19: 792429 .. 798238 (+)
RNA-Seq ExpressionCp4.1LG19g00980
SyntenyCp4.1LG19g00980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAACTCGCCCACATCTTAGTCCCTAACACGCGATTCGAGTCCGAGTTAGCAAGTTCTTGTTGGTTGGTTGTTCTTCCTAAAACCAAAATTCCCTTTTCTTCTCTCAATCTTCAGCTGATCACTCGGGTGATTCTTCATCCGTCAAAGGAATCGAACACGAAGAATGAGGGATTGACGGATCTGTGCTGGTCAATCTCTCTGCTAAGACAATCAATCATAGTCCCCAAGTTGAACAAATCAAGTGATCGGTGTTCCAACTGAGAGACGTAGATGGGTAATTTGTTCGTCAAGAAGCCGAAAGTCACCGATGTTGATAAAGCGATTCTTTCTCTCAAGACGCAGAGGCGCAAACTCGGTCAATATCAACAGCAGGTTTTCAATTCATTCATTCCCTATTCATTTTTTTTTTTTTTAATTTCTTCTTCTTCTCGGCAAGAAGTGTTGCTTTCTGATTAATTTGATTCTGTGGTTGGATTTTTGAAATTGGATTGAATAGAACTAAATATGTTTTCAGAAATTGCTTCTGGTGGTAAAATGTCTGTTAGATCCTAACTTTAGCATTATTAAGTGGGAATTATATGGTAGTTTAGACCCTTGTTGTATGTTCTAGTTCTACAATTACGATATTTCTCTGTGGGAAATACTGTTGATTAAGTAGCTCAATGGTGATTTGAAAATGATTGAAATGCACTAACCTATAAAAGTTGCAACTGTATAATTCTTGGAAATTCTACATAGATCTGAGATTTATTTCTGATGATCTGTTTTCTTGTTAGTGAAGTTTTGTGCTCAAAGTACGGGAGAAATATTATATAATCTCTGACCGTGTGAGTGAGTAGAATCATTCATTGTTGGTCTCTGCATGTACTTGTTGGAATTGGTGCAGATGTCAGGCCCATGTTTGGTCTCTGTTGCCAAGATTTTTTTTGTCACTATTATTTTTCTGCTCTTTTGAGCTGGAGCCCTTTATTGTGATTGGGTAGTTTGTTCTGTTTTTGGCTGCATCATACTTGGACTTTTCATTTTCTGTAATTGCTCTTGTGGGTTATTTATTTTTAATTTCATTCATGAAAGTTGGTTTCCTTATTAAAAAAAAAGAAGAAAAAAACTATGAACCATGAGATTGGATAGCCTGTTTCATAGTGTGATTGCCAGTTGTTCACCATTTAAAACTTAAAAGTAGCTTCTATAAGTTTAATTAAGTTAAACTTAATTAGCGTGACATTTTATGCTTTTTAATGGAGTATATTTAGTGTATTGTATCAAATTTACATATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANTCTATATTTTAAAAATTGATGTATCGCCGTGTTGTGTCCATGTCCGTGCTTCTTAGTTTTCGGGCATCACGATTTTAAAAACACCTTTACAACGAATGTTTCGTTCCCTTCTCCAACTGATGTGGGATCGCACAATCCACCCTCTTTAGGGGCCTAGCGTCTTCGCTGGCACATCGCCTGGTGTTTGGGTCTGATACCATTTGTAACAGCCCAAACCCACTACTAGCAGATATTGTCCTCTTTGGGCTTTCCCTTTCGGGTTTTTAAAACGCATTTGCTAGGGAGAGGTTTCCACACCCTTATAAAGGGTGTTTCGTTCTTCTCCCCAACCGATGTGGGATGTCACAATCCACCCCCCTTCAAGGCTTACGCGTCTACTAGGGAGAGGTTTCCACACCCTTATAAAAGGTGTTTTGTTCTCCTTCCCAACCGATGTGGGATCTCACAGATGTGAAGTAGAGTCTTTGTTTCAAAGTTTTCATTTGTTTCATTAATTGTTTCTTATCTTAGGTGTTTATTTATTTTTTTTCTCTTAGAAAGTTTCTCTCTTTTGTTGTATGTTCGGCAACTTCTCTCGTTCTTTGTGTCACAACTCAACGACACCTCATTTCTTTTTGTTTATAGCTAATACTCTGAATTTTGAAACGTCTAGAACTAATGGATGCTTTGATTGGAATGGCAGTGCAAGCATAGTATGAAATTACTAATTGATTATTTAAATGTTAATAGAATGGAAAGATCATCGTACATTTTTACTTCTTTTCATGTATAAAATATTCACGTTTCATGTTTAGACATCGTATAACTATTTTCCTTGACAAATTATTGCTATATATAATTTATTTGTTCATTTATTTATGTGCACCTCACTTTCCTTAGATGATGCTTTTTGTCGCCTCTCATCTTGAGGTGAGAGAGGCTTGTCACTTTGGTATGTGTTTTGATATGCGTCTTATACTTTAAAAACAAAAATGGGGATCAAACACATTTAAGTCTCATTAATACGTTTATGAATTTCTTTCTAAATAGCCATGGAACTTTAATGATATCTCATCAAATTAATGCATTCTTTTTCAGAAGTGCTTGTGAGCCTTGAAGATATCTCCTTTTATGATGTTCCAACTTCTAAGTCTATTAAAAAATTCTCCTGAAAGTTCATCTCTCATAAGCATTTATGATTTCTCATACTTTTGAATGCTGTCGTAAACTACTCTTTAATTATCTGACTTCAATTTATCCTTTATGACTTCAATTTATCATTTATGAGTATTAATTAAACAATATTTTGAGTCATCTATGAACCATGGAATTGGTTTATGCATGACCACTAATAATCATAAAGAAAAGTTGCAATTTAACCTGGGCGTAAGACCCAAAACTTACAAAATATCTCTAGAAAAGGTCAGACATTACACAAGATGACTTCTTTACGGGTTCATTTCATAGGAATGCAAGTGTATTTTACTTTGAAAATTGAATAATAATTTTTTGTCTCTCCGTGACTTTTTATCCCCTAGCTCGATGCTGTCATTGAAGCAGAAAAGAACGCTGCAAGAGAGTTAGTTCGTCAAAAGAAAAAGGATCGAGCCTTGTTAGCTTTGAAGAAGAAAAAGGCACAAGAAGAATTATTAAAGCAAGTTGATGCCTGGCTGATAAATGTTGAGCAACAAGTGAGTTCTGTTAACTGAACATTTTGTACTTTTTGTTTCTATTCAAAGTAATGGTTGCCTTAGATCTTAAAATCAATATTTTGATGGTTGCAGTAGCCAGGTTTGGTTTTCTGGTTCAACATTAACAATTAAATTTTCTCCTTCGCTATTTGTTTTCCATGTTTAAAAACTCTTTGTAGTTTAGAAATTTTTGTTCGTACCTTGATATAATTGTTGGCTTAGTTGTCAGATATTGAATTGGCCAGCAAACAAAAGGCTGTTTTTGAAAGTTTAAAGGCTGGGAATAATGCGATCAAAGCTATACAAAGTGAAATTAATATAGAAGATGTCCAAAAGCTGATGGATGATACTGCTGAAGCCAAGGCATATCAGGATGTAAGTTCTTTAATTTTCAATTCATATCCTTCAATTTATCAAGTAATTGTAAATATGCCACTATTGACATGTGATATGTATCCCTACTGGTATTATTATAAGCACTAGTTTTCCTTTCATTCCACGGTCCATGCTCGATTAGTTCTTTTCAACCACACATTTACCCTACGATCCTAAACATAACACAACTGGTTAAGACATATACTTACGACCTAGATGTTAGAGATTCAAATCTCCTCACCCAAGATGTTGATTAGATCTTTAAGAAAAAACTTACACTTTTGCTCTCAATTAATTCAAATGTTGTTGTCAAAAACATTGATAAGCACCCATGTTACCCCACCCATGGCCCTGAGTTACCTTTTGAGTAGGGTCTGAATAAGTGATGGCCCACACAAGCTTTGTCGTGCCTTGAAAATCCATGTGTATTCATTTTTTCTCAACATGTCCTTTCCTTCTTTCTTAACTAGTATGAGTTTCAGTGGCTCATCGCGGTTTCCAAATGCGTTTAGGTCTCTAAGTTAGGAAGGAGCCCAATCTTCTTCCCTTGCTCTGATCTTCCTTTGATGTTGCTGCTTCTATCGTTGTGGTCACATCAATGCTATTGGTTATATCCTATTTGGTGCCCACAATCAATCTTTTTTAAGCCTGGCATGTTTCCTTCATTTGTCTTGACTGAGACAATTCCAATGTTCTCATCACCTCAACCTCCATCACAATCTGGGGACCTTCTTCTTTCTTAGCTTTTTGTGACAACAATTTATAATTCTGTGGACCTTTTCTATTTTATTAAGCATGTATTTCTTTTTTTTAGTTTTTCGATGTAATCTTGCATCTGGCCTCAGGCCTAGACTTTGGGGATATCAAAGATTATCTTTATTAATGTGAGATCTCACGTCGGTCGGAGAGGGGAACGAAGGATTCCTTATTGGGGTGTGGAAACCTCTCCCTAGCAGACGTGTTTTAAAAACCGTGAGGCCGACGGCAATATGTAACGGGCCAAAGTGGACAATATATGCTAGCGGTGGGCTTAGAGTGTTACAAGTGTATCAAAGCCAGACACTGGGCGGTGTGCCAGCGAGGACGCTGGCCCCCAAGGAGAATGGATTGTGAGATCTCACATTGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCCCCTAGTAGACGCGTTTTAAAAACTATGAGGCTGACGGCGATACGTAACGGGCCAAAGCGAACAATATCTGCTAGCGGTAGGCTTGGTCTATTACAATTAAGCTCATTGAAGAATCAAATATGCGCTGTTCGAGCAGATGCAGAGACCGTAGGCCTTTGCCAAGGGTTGCCCCTTGGAAGCTATTTTATTAAGCATACATTGGATCCTAGAGTGAGCATGCTCCTTAGTTTGACCATTTTTTTTATGAGAACTCCGTTATAAACTGTTTGATCTTGCCATTTCAGAAACCCTACAGAGAAAATGCTTAATTACTCAAAATTCTTGTTTTGCTGGGCTCTCTTAACCTATTATACCTAATTCGAAGTAGACCAGCATGATTTGAGCTTAAATTTTGTTTCTATTTTTGAAGGAAATCAATGCCATCTTAGGAGAGAAATTATCAGAAGAAGACGAGGAGCAAATCTTAGCAGAATTTGAGAGCTTAGAAGCTCAGGTACTGTGTGTTTTATTGTCTTTTTCATTATTATTTTTCATCTTGCATGATAGGAACCCATATAAGATACAAACTCCTAAGATTGATAGTTGTGGCTGCTCCCGATTGCCTTTTACAATACTATCTCAAGGCGATATTGATATGAACTACCTCTTGTGGAGTGATTCGAATCACGAGTTCAGAAACGAGTTTTCTTTATTCTTAATCTTGATCATCAAGATAATCCGTTCCAAAACTTTCCTTTGGGTTGATTCCATAACAAACCTCTTATCGACTTTTTTCTCAGCTCACCGTTGAAGATCTACCCGAGATCCCAACGTCTAAAGAAAATGGCGAAAGGTTGGATCTTCCCGATGTACCGACCAAAAAACCTGTTGCAGCCGCCGTGGAAGAAGATGCTGAAATTGTTCCATCTGATGCTCCCACAAAGAGAAGAGGTCTCTTTCTCTCTCTCTTTTTCTCTACTCTCTCTCTTTTACATCCACTGGTTTTTTCATAATACATCAAATTTTGTTGGATTGCAGCTATGGAAGAACCGTTGGCAGCATAAGTGTTTGAAGTAGTGGTGAAGTGGTTTGAGCTGAGAATCTGTTCTTTGATGATGTTGTGAATTCTTAGTAATGCTCAGAATGGCTTCATTTACCAATATATGATGTTGTACAGACACCTCCTCATGCACGTATATAAATTGTTCTTCTTTCTGCTTTGGATTGTGTTGTCTAAATGCTTGTTTTTTCTTTCCTTTTCTTTCTTGGATTAAGTTTTCAATTAAATTATATTGTTTTAATTA

mRNA sequence

TAACTCGCCCACATCTTAGTCCCTAACACGCGATTCGAGTCCGAGTTAGCAAGTTCTTGTTGGTTGGTTGTTCTTCCTAAAACCAAAATTCCCTTTTCTTCTCTCAATCTTCAGCTGATCACTCGGGTGATTCTTCATCCGTCAAAGGAATCGAACACGAAGAATGAGGGATTGACGGATCTGTGCTGGTCAATCTCTCTGCTAAGACAATCAATCATAGTCCCCAAGTTGAACAAATCAAGTGATCGGTGTTCCAACTGAGAGACGTAGATGGGTAATTTGTTCGTCAAGAAGCCGAAAGTCACCGATGTTGATAAAGCGATTCTTTCTCTCAAGACGCAGAGGCGCAAACTCGGTCAATATCAACAGCAGCTCGATGCTGTCATTGAAGCAGAAAAGAACGCTGCAAGAGAGTTAGTTCGTCAAAAGAAAAAGGATCGAGCCTTGTTAGCTTTGAAGAAGAAAAAGGCACAAGAAGAATTATTAAAGCAAGTTGATGCCTGGCTGATAAATGTTGAGCAACAATTGTCAGATATTGAATTGGCCAGCAAACAAAAGGCTGTTTTTGAAAGTTTAAAGGCTGGGAATAATGCGATCAAAGCTATACAAAGTGAAATTAATATAGAAGATGTCCAAAAGCTGATGGATGATACTGCTGAAGCCAAGGCATATCAGGATGAAATCAATGCCATCTTAGGAGAGAAATTATCAGAAGAAGACGAGGAGCAAATCTTAGCAGAATTTGAGAGCTTAGAAGCTCAGCTCACCGTTGAAGATCTACCCGAGATCCCAACGTCTAAAGAAAATGGCGAAAGGTTGGATCTTCCCGATGTACCGACCAAAAAACCTGTTGCAGCCGCCGTGGAAGAAGATGCTGAAATTGTTCCATCTGATGCTCCCACAAAGAGAAGAGCTATGGAAGAACCGTTGGCAGCATAAGTGTTTGAAGTAGTGGTGAAGTGGTTTGAGCTGAGAATCTGTTCTTTGATGATGTTGTGAATTCTTAGTAATGCTCAGAATGGCTTCATTTACCAATATATGATGTTGTACAGACACCTCCTCATGCACGTATATAAATTGTTCTTCTTTCTGCTTTGGATTGTGTTGTCTAAATGCTTGTTTTTTCTTTCCTTTTCTTTCTTGGATTAAGTTTTCAATTAAATTATATTGTTTTAATTA

Coding sequence (CDS)

ATGGGTAATTTGTTCGTCAAGAAGCCGAAAGTCACCGATGTTGATAAAGCGATTCTTTCTCTCAAGACGCAGAGGCGCAAACTCGGTCAATATCAACAGCAGCTCGATGCTGTCATTGAAGCAGAAAAGAACGCTGCAAGAGAGTTAGTTCGTCAAAAGAAAAAGGATCGAGCCTTGTTAGCTTTGAAGAAGAAAAAGGCACAAGAAGAATTATTAAAGCAAGTTGATGCCTGGCTGATAAATGTTGAGCAACAATTGTCAGATATTGAATTGGCCAGCAAACAAAAGGCTGTTTTTGAAAGTTTAAAGGCTGGGAATAATGCGATCAAAGCTATACAAAGTGAAATTAATATAGAAGATGTCCAAAAGCTGATGGATGATACTGCTGAAGCCAAGGCATATCAGGATGAAATCAATGCCATCTTAGGAGAGAAATTATCAGAAGAAGACGAGGAGCAAATCTTAGCAGAATTTGAGAGCTTAGAAGCTCAGCTCACCGTTGAAGATCTACCCGAGATCCCAACGTCTAAAGAAAATGGCGAAAGGTTGGATCTTCCCGATGTACCGACCAAAAAACCTGTTGCAGCCGCCGTGGAAGAAGATGCTGAAATTGTTCCATCTGATGCTCCCACAAAGAGAAGAGCTATGGAAGAACCGTTGGCAGCATAA

Protein sequence

MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALLALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIEDVQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENGERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA
Homology
BLAST of Cp4.1LG19g00980 vs. ExPASy Swiss-Prot
Match: Q8GXN6 (Vacuolar protein sorting-associated protein 20 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS20.1 PE=1 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 7.3e-81
Identity = 163/223 (73.09%), Postives = 200/223 (89.69%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKP++T+VD+AILSLKTQRRKLGQYQQ+L+ VIEAEK AAR+L+R+K+KDRALL
Sbjct: 1   MGNLFVKKPQITEVDRAILSLKTQRRKLGQYQQKLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           AL+KK+ QEELLKQVD W+INVEQQL+DIEL SKQKAVFESLK GN+AIKAIQSE++++D
Sbjct: 61  ALRKKRTQEELLKQVDQWVINVEQQLTDIELTSKQKAVFESLKQGNSAIKAIQSELDLDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSK-EN 180
           VQKLMDDTA+AKAYQDE+NAILGEKLS EDEE ILAEF++LE+QL V+++PE+PT + E 
Sbjct: 121 VQKLMDDTADAKAYQDELNAILGEKLSAEDEEDILAEFDNLESQLIVDEMPEVPTKESEE 180

Query: 181 GERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 223
            E+LDLPDVPTK PVA+    +AEI P+++ TK + +EEPL A
Sbjct: 181 SEKLDLPDVPTKTPVAS----NAEITPAESATKTKVLEEPLPA 219

BLAST of Cp4.1LG19g00980 vs. ExPASy Swiss-Prot
Match: Q9FY89 (Vacuolar protein sorting-associated protein 20 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS20.2 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 3.6e-80
Identity = 166/225 (73.78%), Postives = 194/225 (86.22%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPK+T+VD+AILSLKTQRRKLGQYQQQL+ VIEAEK AAR+L+R+K+KDRALL
Sbjct: 1   MGNLFVKKPKITEVDRAILSLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKK+ QEELLKQVD WLINVEQQL+DIEL SKQKAVFESLK GNNAIKAIQSE+N++D
Sbjct: 61  ALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSK--- 180
           VQKLMDDTAEAKAYQDE++AILGEKLS EDEE+ILAEF++LE+ L VED+PE+PT++   
Sbjct: 121 VQKLMDDTAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPTTELMP 180

Query: 181 ENGERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 223
           E  E++DLPDVPTK PVA+              TKR+ +EEPL A
Sbjct: 181 EEPEKMDLPDVPTKAPVAS---------NETTSTKRKVLEEPLEA 216

BLAST of Cp4.1LG19g00980 vs. ExPASy Swiss-Prot
Match: Q503V0 (Charged multivesicular body protein 6 OS=Danio rerio OX=7955 GN=chmp6 PE=2 SV=3)

HSP 1 Score: 137.1 bits (344), Expect = 2.4e-31
Identity = 83/196 (42.35%), Postives = 133/196 (67.86%), Query Frame = 0

Query: 1   MGNLFVKK--PKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRA 60
           MGNLF KK   +VT+ D+A+L LK QR KL QYQ+++   ++ E+  A++L++  KKD+A
Sbjct: 1   MGNLFGKKKATRVTEQDRAVLQLKQQRDKLKQYQKRITLQMDKERQLAKQLLKDGKKDKA 60

Query: 61  LLALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI 120
           LL LKKK+ Q++LL++ +  + N+E+ + DIE A  +  V E LK GN+ +K +   ++I
Sbjct: 61  LLLLKKKRYQDQLLEKTENQISNLERMVQDIEFAQIEMKVIEGLKVGNDCLKKMHEVLSI 120

Query: 121 EDVQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKE 180
           E+V+K+MD+T +A  YQ +I+ +L   L++EDEE +LAE E++       +LPE+P    
Sbjct: 121 EEVEKIMDETHDAIEYQKQIDEMLAGSLTQEDEEAVLAELEAITQGEADLELPEVP---- 180

Query: 181 NGERLDLPDVPTKKPV 195
            GE  +LP+VP ++PV
Sbjct: 181 -GE--ELPEVPEQEPV 189

BLAST of Cp4.1LG19g00980 vs. ExPASy Swiss-Prot
Match: Q5ZL55 (Charged multivesicular body protein 6 OS=Gallus gallus OX=9031 GN=CHMP6 PE=2 SV=3)

HSP 1 Score: 126.7 bits (317), Expect = 3.2e-28
Identity = 79/200 (39.50%), Postives = 128/200 (64.00%), Query Frame = 0

Query: 1   MGNLFVKK--PKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRA 60
           MGNLF +K   +VT+ DKA+L LK QR KL QYQ+++   +E E+  AR+L+++ KK++A
Sbjct: 1   MGNLFGRKRRSRVTEQDKAVLQLKQQRDKLRQYQKRISLGLERERELARQLLKEGKKEKA 60

Query: 61  LLALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI 120
           +L LKKK+ QE+LL + D  + N+E+ + DIE    +  V E LK GN  +  +   ++I
Sbjct: 61  MLLLKKKRYQEQLLDKTDNQISNLERMVQDIEFTQIEMKVIEGLKIGNECLNKMHQVMSI 120

Query: 121 EDVQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESL-EAQLTVEDLPEIPTSK 180
           E+V++++ +T +A  YQ +I+ IL   L+EEDE+ IL E  ++ + QL + D+P  P  +
Sbjct: 121 EEVERIIGETQDAVEYQRQIDEILAGSLTEEDEDAILEELNAITQEQLELPDVPSEPLPE 180

Query: 181 ENGERLDLPDVPTKKPVAAA 198
           E  E   + + P  + VAA+
Sbjct: 181 EPPEATPVKNRPKPELVAAS 200

BLAST of Cp4.1LG19g00980 vs. ExPASy Swiss-Prot
Match: Q6GMA4 (Charged multivesicular body protein 6-A OS=Xenopus laevis OX=8355 GN=chmp6-a PE=2 SV=3)

HSP 1 Score: 124.0 bits (310), Expect = 2.1e-27
Identity = 83/199 (41.71%), Postives = 125/199 (62.81%), Query Frame = 0

Query: 1   MGNLFVKK--PKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRA 60
           MGNLF +K   +VT+ DKA+L LK QR KL QYQ+++   ++ E+  A++L+   KK++A
Sbjct: 1   MGNLFARKRRSRVTEQDKAVLQLKQQRDKLKQYQKKITLQLQRERELAKQLLHDGKKEKA 60

Query: 61  LLALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI 120
            L LKKK+ QE+LL++ D  + N+E+ + DIE A  +  V E LK GN  +K +   ++I
Sbjct: 61  KLLLKKKRYQEQLLEKTDNQISNLEKMVEDIEFAQIEMKVIEGLKVGNECLKKMHEVMSI 120

Query: 121 EDVQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVED--LPEIPTS 180
           E+V+++MD+T E   YQ +I+ +L   L+ EDEE IL E E+    +T ED  LPE P+ 
Sbjct: 121 EEVERIMDETQEGIEYQRQIDEMLSGSLTAEDEEAILEELEA----ITQEDLELPEAPSE 180

Query: 181 KENGERLDLPD-VPTKKPV 195
                   LPD +P K+ V
Sbjct: 181 -------PLPDTIPEKQAV 188

BLAST of Cp4.1LG19g00980 vs. NCBI nr
Match: XP_023518938.1 (vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394 bits (1013), Expect = 1.34e-137
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180

Query: 181 ERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 222
           ERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA
Sbjct: 181 ERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 222

BLAST of Cp4.1LG19g00980 vs. NCBI nr
Match: XP_022925284.1 (vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita moschata] >KAG6595610.1 Vacuolar protein sorting-associated protein 20-like 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027582.1 Vacuolar protein sorting-associated protein 20-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387 bits (993), Expect = 1.61e-134
Identity = 221/224 (98.66%), Postives = 221/224 (98.66%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180

Query: 181 ERLDLPDVPTKKPVA--AAVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E LDLPDVPTKKPVA  AAVEEDAEIVPSDAPTKRRAMEEPLAA
Sbjct: 181 EGLDLPDVPTKKPVASAAAVEEDAEIVPSDAPTKRRAMEEPLAA 224

BLAST of Cp4.1LG19g00980 vs. NCBI nr
Match: XP_022966453.1 (vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita maxima])

HSP 1 Score: 384 bits (987), Expect = 1.32e-133
Identity = 219/224 (97.77%), Postives = 220/224 (98.21%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVD WLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED
Sbjct: 61  ALKKKKAQEELLKQVDVWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIP +KENG
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPMAKENG 180

Query: 181 ERLDLPDVPTKKPVAAA--VEEDAEIVPSDAPTKRRAMEEPLAA 222
           ERLDLPDVPTKKPVAAA  VEEDAEIVPSDAPTKRRAMEEPLAA
Sbjct: 181 ERLDLPDVPTKKPVAAAAAVEEDAEIVPSDAPTKRRAMEEPLAA 224

BLAST of Cp4.1LG19g00980 vs. NCBI nr
Match: XP_038883019.1 (vacuolar protein sorting-associated protein 20 homolog 2-like [Benincasa hispida])

HSP 1 Score: 370 bits (949), Expect = 7.86e-128
Identity = 210/223 (94.17%), Postives = 217/223 (97.31%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI+D
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQL V+DLPE+PTS+EN 
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLIVQDLPEVPTSEENA 180

Query: 181 ERLDLPDVPTKKPVAA-AVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E+L LPDVP+KKPVAA A+EEDAEIV SDAPTKRR MEEPLAA
Sbjct: 181 EKLVLPDVPSKKPVAADAIEEDAEIVSSDAPTKRRVMEEPLAA 223

BLAST of Cp4.1LG19g00980 vs. NCBI nr
Match: XP_023520722.1 (vacuolar protein sorting-associated protein 20 homolog 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 368 bits (945), Expect = 3.31e-127
Identity = 210/224 (93.75%), Postives = 216/224 (96.43%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI+D
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPE+PTS+EN 
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEVPTSEENA 180

Query: 181 ERLDLPDVPTKKPVAA--AVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E+LDLP+VPTKKPVAA  A+EEDA IV SDA TKRR MEEPL A
Sbjct: 181 EKLDLPNVPTKKPVAADDAIEEDAVIVSSDASTKRRVMEEPLPA 224

BLAST of Cp4.1LG19g00980 vs. ExPASy TrEMBL
Match: A0A6J1EBQ2 (vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432566 PE=4 SV=1)

HSP 1 Score: 387 bits (993), Expect = 7.79e-135
Identity = 221/224 (98.66%), Postives = 221/224 (98.66%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180

Query: 181 ERLDLPDVPTKKPVA--AAVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E LDLPDVPTKKPVA  AAVEEDAEIVPSDAPTKRRAMEEPLAA
Sbjct: 181 EGLDLPDVPTKKPVASAAAVEEDAEIVPSDAPTKRRAMEEPLAA 224

BLAST of Cp4.1LG19g00980 vs. ExPASy TrEMBL
Match: A0A6J1HS74 (vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466101 PE=4 SV=1)

HSP 1 Score: 384 bits (987), Expect = 6.40e-134
Identity = 219/224 (97.77%), Postives = 220/224 (98.21%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVD WLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED
Sbjct: 61  ALKKKKAQEELLKQVDVWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIP +KENG
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPMAKENG 180

Query: 181 ERLDLPDVPTKKPVAAA--VEEDAEIVPSDAPTKRRAMEEPLAA 222
           ERLDLPDVPTKKPVAAA  VEEDAEIVPSDAPTKRRAMEEPLAA
Sbjct: 181 ERLDLPDVPTKKPVAAAAAVEEDAEIVPSDAPTKRRAMEEPLAA 224

BLAST of Cp4.1LG19g00980 vs. ExPASy TrEMBL
Match: A0A6J1D5M8 (vacuolar protein sorting-associated protein 20 homolog 2-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017847 PE=4 SV=1)

HSP 1 Score: 367 bits (943), Expect = 3.12e-127
Identity = 208/223 (93.27%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAA+ELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAAKELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEIN++D
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINLDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQ+TV+DLPE+PTS EN 
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQITVQDLPEVPTSNENV 180

Query: 181 ERLDLPDVPTKKPVAA-AVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E+LDLPDVPTKKPVAA  +EEDAEIV S APTKRR MEEPLAA
Sbjct: 181 EKLDLPDVPTKKPVAADPIEEDAEIVSSVAPTKRRVMEEPLAA 223

BLAST of Cp4.1LG19g00980 vs. ExPASy TrEMBL
Match: A0A6J1EPH4 (vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435445 PE=4 SV=1)

HSP 1 Score: 366 bits (939), Expect = 1.27e-126
Identity = 208/223 (93.27%), Postives = 215/223 (96.41%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI+D
Sbjct: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDE+NAILGEKLSEEDEEQILAEFE LEAQLTVEDLPE+PTS+EN 
Sbjct: 121 VQKLMDDTAEAKAYQDEMNAILGEKLSEEDEEQILAEFEGLEAQLTVEDLPEVPTSEENA 180

Query: 181 ERLDLPDVPTKKPVAA-AVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E+LDLP+VPTKKPVAA A+EEDA IV SDA TKRR MEEPL A
Sbjct: 181 EKLDLPNVPTKKPVAADAIEEDAVIVSSDASTKRRVMEEPLPA 223

BLAST of Cp4.1LG19g00980 vs. ExPASy TrEMBL
Match: A0A1S3B5R7 (vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucumis melo OX=3656 GN=LOC103486109 PE=4 SV=1)

HSP 1 Score: 362 bits (930), Expect = 2.99e-125
Identity = 207/223 (92.83%), Postives = 214/223 (95.96%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL
Sbjct: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKKKAQEELLK VDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINI+D
Sbjct: 61  ALKKKKAQEELLKHVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSKENG 180
           VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPE+PTS+E  
Sbjct: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEVPTSEEKE 180

Query: 181 ERLDLPDVPTKKPVAA-AVEEDAEIVPSDAPTKRRAMEEPLAA 222
           E+LDLP+VP+KKPVAA A+EEDAE V SDA TKRR ME PLAA
Sbjct: 181 EKLDLPEVPSKKPVAADAIEEDAETVSSDASTKRRVMEGPLAA 223

BLAST of Cp4.1LG19g00980 vs. TAIR 10
Match: AT5G63880.1 (SNF7 family protein )

HSP 1 Score: 301.6 bits (771), Expect = 5.2e-82
Identity = 163/223 (73.09%), Postives = 200/223 (89.69%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKP++T+VD+AILSLKTQRRKLGQYQQ+L+ VIEAEK AAR+L+R+K+KDRALL
Sbjct: 1   MGNLFVKKPQITEVDRAILSLKTQRRKLGQYQQKLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           AL+KK+ QEELLKQVD W+INVEQQL+DIEL SKQKAVFESLK GN+AIKAIQSE++++D
Sbjct: 61  ALRKKRTQEELLKQVDQWVINVEQQLTDIELTSKQKAVFESLKQGNSAIKAIQSELDLDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSK-EN 180
           VQKLMDDTA+AKAYQDE+NAILGEKLS EDEE ILAEF++LE+QL V+++PE+PT + E 
Sbjct: 121 VQKLMDDTADAKAYQDELNAILGEKLSAEDEEDILAEFDNLESQLIVDEMPEVPTKESEE 180

Query: 181 GERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 223
            E+LDLPDVPTK PVA+    +AEI P+++ TK + +EEPL A
Sbjct: 181 SEKLDLPDVPTKTPVAS----NAEITPAESATKTKVLEEPLPA 219

BLAST of Cp4.1LG19g00980 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 299.3 bits (765), Expect = 2.6e-81
Identity = 166/225 (73.78%), Postives = 194/225 (86.22%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKPK+T+VD+AILSLKTQRRKLGQYQQQL+ VIEAEK AAR+L+R+K+KDRALL
Sbjct: 1   MGNLFVKKPKITEVDRAILSLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           ALKKK+ QEELLKQVD WLINVEQQL+DIEL SKQKAVFESLK GNNAIKAIQSE+N++D
Sbjct: 61  ALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLDD 120

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKLSEEDEEQILAEFESLEAQLTVEDLPEIPTSK--- 180
           VQKLMDDTAEAKAYQDE++AILGEKLS EDEE+ILAEF++LE+ L VED+PE+PT++   
Sbjct: 121 VQKLMDDTAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPTTELMP 180

Query: 181 ENGERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 223
           E  E++DLPDVPTK PVA+              TKR+ +EEPL A
Sbjct: 181 EEPEKMDLPDVPTKAPVAS---------NETTSTKRKVLEEPLEA 216

BLAST of Cp4.1LG19g00980 vs. TAIR 10
Match: AT5G63880.2 (SNF7 family protein )

HSP 1 Score: 288.1 bits (736), Expect = 6.0e-78
Identity = 163/247 (65.99%), Postives = 200/247 (80.97%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           MGNLFVKKP++T+VD+AILSLKTQRRKLGQYQQ+L+ VIEAEK AAR+L+R+K+KDRALL
Sbjct: 1   MGNLFVKKPQITEVDRAILSLKTQRRKLGQYQQKLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
           AL+KK+ QEELLKQVD W+INVEQQL+DIEL SKQKAVFESLK GN+AIKAIQSE++++D
Sbjct: 61  ALRKKRTQEELLKQVDQWVINVEQQLTDIELTSKQKAVFESLKQGNSAIKAIQSELDLDD 120

Query: 121 VQKLMDDTAEAKAYQD------------------------EINAILGEKLSEEDEEQILA 180
           VQKLMDDTA+AKAYQD                        E+NAILGEKLS EDEE ILA
Sbjct: 121 VQKLMDDTADAKAYQDCENLYNLFCRAFVFDDSIYISECQELNAILGEKLSAEDEEDILA 180

Query: 181 EFESLEAQLTVEDLPEIPTSK-ENGERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRA 223
           EF++LE+QL V+++PE+PT + E  E+LDLPDVPTK PVA+    +AEI P+++ TK + 
Sbjct: 181 EFDNLESQLIVDEMPEVPTKESEESEKLDLPDVPTKTPVAS----NAEITPAESATKTKV 240

BLAST of Cp4.1LG19g00980 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 55.1 bits (131), Expect = 8.5e-08
Identity = 52/187 (27.81%), Postives = 91/187 (48.66%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           M  LF K  + T   + +  L      L + +  L      E   A+E  R K K  A+ 
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
            LK+K+  E+ ++Q+  + + +  Q+  +E A       ++L+ G +A+KA+Q   NI+D
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 121 VQKLMDD----TAEAKAYQDEINAILGEKLSEEDEEQILAEFESLE-AQLTVEDLPEIPT 180
           V K MD+    T   K  Q+ ++A  G   ++ DE+++ AE + LE A+L  + L  +P 
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG--ANDFDEDELEAELDELEGAELEEQLLQPVPI 182

Query: 181 SKENGER 183
               G +
Sbjct: 183 HVPQGNK 187

BLAST of Cp4.1LG19g00980 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 53.1 bits (126), Expect = 3.2e-07
Identity = 63/225 (28.00%), Postives = 104/225 (46.22%), Query Frame = 0

Query: 1   MGNLFVKKPKVTDVDKAILSLKTQRRKLGQYQQQLDAVIEAEKNAARELVRQKKKDRALL 60
           M  LF K  +  +  + +  L      L + ++ L     AE   A+E  R K K  A+ 
Sbjct: 2   MNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQ 61

Query: 61  ALKKKKAQEELLKQVDAWLINVEQQLSDIELASKQKAVFESLKAGNNAIKAIQSEINIED 120
            LK+K+  E  ++Q+  + + +  Q+  +E A       ++L++G +A+KA+Q   NI+D
Sbjct: 62  CLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNIDD 121

Query: 121 VQKLMDDTAEAKAYQDEINAILGEKL---SEEDEEQILAEFESLEAQLTVEDLPEIPTSK 180
           V K MD+  E      +I   L   +   ++ DE+++ AE + LE++     L +  T+ 
Sbjct: 122 VDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATTA 181

Query: 181 ENGERLDLPDVPTKKPVAAAVEEDAEIVPSDAPTKRRAMEEPLAA 223
                  LP VP       A  + A  VP     KR A EE LAA
Sbjct: 182 P-----PLPSVP-----VPAGRQPARPVPQ----KRTAEEEELAA 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GXN67.3e-8173.09Vacuolar protein sorting-associated protein 20 homolog 1 OS=Arabidopsis thaliana... [more]
Q9FY893.6e-8073.78Vacuolar protein sorting-associated protein 20 homolog 2 OS=Arabidopsis thaliana... [more]
Q503V02.4e-3142.35Charged multivesicular body protein 6 OS=Danio rerio OX=7955 GN=chmp6 PE=2 SV=3[more]
Q5ZL553.2e-2839.50Charged multivesicular body protein 6 OS=Gallus gallus OX=9031 GN=CHMP6 PE=2 SV=... [more]
Q6GMA42.1e-2741.71Charged multivesicular body protein 6-A OS=Xenopus laevis OX=8355 GN=chmp6-a PE=... [more]
Match NameE-valueIdentityDescription
XP_023518938.11.34e-137100.00vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita pepo su... [more]
XP_022925284.11.61e-13498.66vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita moschat... [more]
XP_022966453.11.32e-13397.77vacuolar protein sorting-associated protein 20 homolog 2-like [Cucurbita maxima][more]
XP_038883019.17.86e-12894.17vacuolar protein sorting-associated protein 20 homolog 2-like [Benincasa hispida... [more]
XP_023520722.13.31e-12793.75vacuolar protein sorting-associated protein 20 homolog 2-like isoform X2 [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1EBQ27.79e-13598.66vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita mosch... [more]
A0A6J1HS746.40e-13497.77vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita maxim... [more]
A0A6J1D5M83.12e-12793.27vacuolar protein sorting-associated protein 20 homolog 2-like isoform X1 OS=Momo... [more]
A0A6J1EPH41.27e-12693.27vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucurbita mosch... [more]
A0A1S3B5R72.99e-12592.83vacuolar protein sorting-associated protein 20 homolog 2-like OS=Cucumis melo OX... [more]
Match NameE-valueIdentityDescription
AT5G63880.15.2e-8273.09SNF7 family protein [more]
AT5G09260.12.6e-8173.78vacuolar protein sorting-associated protein 20.2 [more]
AT5G63880.26.0e-7865.99SNF7 family protein [more]
AT2G19830.18.5e-0827.81SNF7 family protein [more]
AT4G29160.13.2e-0728.00SNF7 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 50..70
NoneNo IPR availableCOILSCoilCoilcoord: 21..41
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..90
e-value: 3.8E-10
score: 41.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..222
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 198..222
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 2..222
NoneNo IPR availablePANTHERPTHR22761:SF50VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 20 HOMOLOG 1coord: 2..222
IPR005024Snf7 familyPFAMPF03357Snf7coord: 16..192
e-value: 9.5E-41
score: 139.3

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g00980.1Cp4.1LG19g00980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body