Cp4.1LG18g09280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g09280
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMagnesium protoporphyrin IX methyltransferase
LocationCp4.1LG18: 8294040 .. 8295219 (+)
RNA-Seq ExpressionCp4.1LG18g09280
SyntenyCp4.1LG18g09280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCACTCCGCATTTCCATATCCCACACTCTGCTTCCACCCCGACTCTACCACAAATTCTCGATTCTTCCTGTCTCCACCCCCTCTCAAGCTTGTTAAATTTTCAAGAACGCCCAATGTTAATGCGCCTGCGCCTGCGCCTGCGCCTGCCCCACCACTCTCCGCCGTAGCTACCGAACTCCCCGTCGTCGACGGGGCAACAATTGCAGCGATCGGCGGCGGTTCGGTTGCCGCGCTCGCTGCCGTTCTATCTCTCACCGACCCGGAGAGACGTCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTCAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATATGGAGAGACTGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGGCATGTTGAAAGATGAGGGCCCACTCACAGGAGTAACAGTCTGCGATGCCGGCTGTGGAACCGGTAGCTTATCCATTCCGCTGGCCAAGGAAGGTGCCATCGTCTTCGCCAGTGATATTTCTGCTGCTATGGTCGCCGAGGCCCAAAACAAGGTGCGCACCGTTGCTTCCCCTACTTCCTTTCTCCTTCTACTGTAATTTATCTGTCCAGTGTTCTAAAATGCATCCGCATCCCTCCGTGTGTATGTGAAATTGAATGTGAGATATAGTTGCTTTCTGTGTTGGCAAAAAAAAAAAAAAAAAAAAAAAGAAAAAGAAAGAAATTGAATGTGGGATTAAACTGGCGCAGGCAAAAGAAGAGGTTGCGGGAGGCGTGGTGCCAAAATTTGAAGTGAAGGATTTGGAGAGCTTGGAAGGGAAGTATGATACGGTAGTATGTCTGGATGTTTTGATACACTATCCCCAGAACAAGGCAGACGGCATGATTGCGCACCTTGCATCCTTAGCTGAAAAGCGTTTAATCATAAGCTTTGCGCCCAAAACATTTTACTATGATCTGCTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCGGAGGCAGACGTGGAGACCGCGTTGCGGAGGGTTGGCTGGAAAATTAGGAAGAGAGGTCTAGTCTCCACCCAGTTTTACTTTGCCACACTGGTTGAGGCAGTTCCTGCTTGA

mRNA sequence

ATGGCTCACTCCGCATTTCCATATCCCACACTCTGCTTCCACCCCGACTCTACCACAAATTCTCGATTCTTCCTGTCTCCACCCCCTCTCAAGCTTGTTAAATTTTCAAGAACGCCCAATGTTAATGCGCCTGCGCCTGCGCCTGCGCCTGCCCCACCACTCTCCGCCGTAGCTACCGAACTCCCCGTCGTCGACGGGGCAACAATTGCAGCGATCGGCGGCGGTTCGGTTGCCGCGCTCGCTGCCGTTCTATCTCTCACCGACCCGGAGAGACGTCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTCAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATATGGAGAGACTGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGGCATGTTGAAAGATGAGGGCCCACTCACAGGAGTAACAGTCTGCGATGCCGGCTGTGGAACCGGTAGCTTATCCATTCCGCTGGCCAAGGAAGGTGCCATCGTCTTCGCCAGTGATATTTCTGCTGCTATGGTCGCCGAGGCCCAAAACAAGGCAAAAGAAGAGGTTGCGGGAGGCGTGGTGCCAAAATTTGAAGTGAAGGATTTGGAGAGCTTGGAAGGGAAGTATGATACGGTAGTATGTCTGGATGTTTTGATACACTATCCCCAGAACAAGGCAGACGGCATGATTGCGCACCTTGCATCCTTAGCTGAAAAGCGTTTAATCATAAGCTTTGCGCCCAAAACATTTTACTATGATCTGCTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCGGAGGCAGACGTGGAGACCGCGTTGCGGAGGGTTGGCTGGAAAATTAGGAAGAGAGGTCTAGTCTCCACCCAGTTTTACTTTGCCACACTGGTTGAGGCAGTTCCTGCTTGA

Coding sequence (CDS)

ATGGCTCACTCCGCATTTCCATATCCCACACTCTGCTTCCACCCCGACTCTACCACAAATTCTCGATTCTTCCTGTCTCCACCCCCTCTCAAGCTTGTTAAATTTTCAAGAACGCCCAATGTTAATGCGCCTGCGCCTGCGCCTGCGCCTGCCCCACCACTCTCCGCCGTAGCTACCGAACTCCCCGTCGTCGACGGGGCAACAATTGCAGCGATCGGCGGCGGTTCGGTTGCCGCGCTCGCTGCCGTTCTATCTCTCACCGACCCGGAGAGACGTCGCCGCCTGCAGGCCGAAGAGGTGGGTGGTGGCGACAAGGATGTGGTGAAGGAATACTTCAACAATTCGGGGTTTCAGAGGTGGAAGAAAATATATGGAGAGACTGATGATGTGAACCGGGTGCAGCGAGACATTAGGTTGGGTCACTCCAAGACGGTGGAGAATGTGATGGGCATGTTGAAAGATGAGGGCCCACTCACAGGAGTAACAGTCTGCGATGCCGGCTGTGGAACCGGTAGCTTATCCATTCCGCTGGCCAAGGAAGGTGCCATCGTCTTCGCCAGTGATATTTCTGCTGCTATGGTCGCCGAGGCCCAAAACAAGGCAAAAGAAGAGGTTGCGGGAGGCGTGGTGCCAAAATTTGAAGTGAAGGATTTGGAGAGCTTGGAAGGGAAGTATGATACGGTAGTATGTCTGGATGTTTTGATACACTATCCCCAGAACAAGGCAGACGGCATGATTGCGCACCTTGCATCCTTAGCTGAAAAGCGTTTAATCATAAGCTTTGCGCCCAAAACATTTTACTATGATCTGCTGAAGAGGGTGGGAGAGTTGTTCCCCGGACCTTCCAAGGCAACCAGGGCATATCTGCACTCGGAGGCAGACGTGGAGACCGCGTTGCGGAGGGTTGGCTGGAAAATTAGGAAGAGAGGTCTAGTCTCCACCCAGTTTTACTTTGCCACACTGGTTGAGGCAGTTCCTGCTTGA

Protein sequence

MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVPA
Homology
BLAST of Cp4.1LG18g09280 vs. ExPASy Swiss-Prot
Match: Q9SW18 (Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CHLM PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.4e-119
Identity = 220/295 (74.58%), Postives = 251/295 (85.08%), Query Frame = 0

Query: 32  LVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPER 91
           L +F      N   P    A  ++A  T+L  VD  TIA +GGGSVAALAA++SLTDPER
Sbjct: 18  LPRFPNATRFNV-TPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAMVSLTDPER 77

Query: 92  RRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGM 151
           RR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+KTVEN M M
Sbjct: 78  RRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAKTVENTMLM 137

Query: 152 LKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVP 211
           L ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK ++    +P
Sbjct: 138 LTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKAQLPSENLP 197

Query: 212 KFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLL 271
           KFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAPKTFYYD+L
Sbjct: 198 KFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAPKTFYYDIL 257

Query: 272 KRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
           KR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+EAVP
Sbjct: 258 KRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIEAVP 311

BLAST of Cp4.1LG18g09280 vs. ExPASy Swiss-Prot
Match: Q55467 (Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=chlM PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 5.6e-69
Identity = 128/222 (57.66%), Postives = 168/222 (75.68%), Query Frame = 0

Query: 104 DKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTV 163
           DK +V++YFN++GF RW++IYG+   VN VQ+DIR+GH +TV++V+  L  +G L G+ V
Sbjct: 8   DKTIVRDYFNSTGFDRWRRIYGD-GQVNFVQKDIRVGHQQTVDSVVAWLVADGNLPGLLV 67

Query: 164 CDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEG 223
           CDAGCG GSLSIPLA+ GA+V+ SDIS  MV EAQ KA+E +A G  P F  +DL  L G
Sbjct: 68  CDAGCGVGSLSIPLAQAGALVYGSDISEKMVGEAQQKAQEVLAYGNQPTFMTQDLAQLGG 127

Query: 224 KYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSK 283
           KYDTV+CLDVLIHYP  +A  MI+HLASLA++RLI+SFAPKT    +LK++G LFPGPSK
Sbjct: 128 KYDTVICLDVLIHYPTEEASAMISHLASLADRRLILSFAPKTLGLTVLKKIGGLFPGPSK 187

Query: 284 ATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAV 326
            TRAY H EAD+   L   G+ I + G+ ST+FY++ ++EAV
Sbjct: 188 TTRAYQHKEADIRKILGDNGFSIARTGMTSTRFYYSRILEAV 228

BLAST of Cp4.1LG18g09280 vs. ExPASy Swiss-Prot
Match: P26236 (Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 GN=bchM PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 4.5e-18
Identity = 63/220 (28.64%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 108 VKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAG 167
           V+ YF+ +  + W ++    + V++V++ +R G       ++  L D+  LTG  V DAG
Sbjct: 12  VEHYFDRTATRAWARLTTADEKVSKVRQTVREGRDTMRAVMLSRLPDD--LTGCRVMDAG 71

Query: 168 CGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDL-ESLEGKYD 227
           CGTG  ++ LA+ GA V A DIS  ++  A+++   E+ G V   F V D+ +   G++D
Sbjct: 72  CGTGLTTVELARRGADVVAVDISPQLIDIAKDRLPPELRGKV--SFHVGDMADPALGQFD 131

Query: 228 TVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATR 287
            VV +D LI+Y       ++  L       ++ + APKT +      +G+LFP  +++  
Sbjct: 132 YVVAMDSLIYYRAPDIGRVLTELGKRTHSAIVFTVAPKTAFLMAFWWLGKLFPRSNRSPV 191

Query: 288 AYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
              H+   ++   R  G  + K   V+  FY +  +E  P
Sbjct: 192 MIPHA---LDKLQRHAGDSLIKIDRVARGFYISECLEYRP 224

BLAST of Cp4.1LG18g09280 vs. ExPASy Swiss-Prot
Match: Q9PAM5 (Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain 9a5c) OX=160492 GN=ubiG PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 5.7e-05
Identity = 39/124 (31.45%), Postives = 60/124 (48.39%), Query Frame = 0

Query: 157 PLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVK 216
           PL+G  V D GCG G LS  LA++GA V A D++  ++  A+    E    G+   + ++
Sbjct: 53  PLSGARVLDVGCGGGLLSEALARQGAHVTAIDLAPELIKVARLHGLE---SGIQVDYRIQ 112

Query: 217 DLESL----EGKYDTVVCLDVLIHYPQNKA-DGMIAHLASLAEKRLIISFAPKTFYYDLL 276
            +E L       +D + C+++L H P   A     AHL      RL +S   +T    +L
Sbjct: 113 AIEDLLAEQPAPFDAIACMEMLEHVPDPAAIVDACAHLLK-PGGRLFVSTINRTLAAFML 172

BLAST of Cp4.1LG18g09280 vs. ExPASy Swiss-Prot
Match: A7MPA9 (Ubiquinone biosynthesis O-methyltransferase OS=Cronobacter sakazakii (strain ATCC BAA-894) OX=290339 GN=ubiG PE=3 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 7.4e-05
Identity = 40/125 (32.00%), Postives = 59/125 (47.20%), Query Frame = 0

Query: 119 RWKKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLA 178
           RW    GE   ++R+   +RLG+         + +  G L G  V D GCG G LS  +A
Sbjct: 26  RWWDTEGEFKPLHRI-NPLRLGY---------IAERAGGLFGKKVLDVGCGGGILSESMA 85

Query: 179 KEGAIVFASDISAAMVAEAQNKAKEE-VAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHY 238
           +EGA V   D+ A  +A A+  A E  V    V +   +      G YD V C+++L H 
Sbjct: 86  REGANVTGLDMGAEPLAVARLHALESGVELNYVQQTVEEHAAQHAGAYDVVTCMEMLEHV 140

Query: 239 PQNKA 243
           P  ++
Sbjct: 146 PDPRS 140

BLAST of Cp4.1LG18g09280 vs. NCBI nr
Match: XP_023516229.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo] >KAG6589339.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023025.1 Magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628 bits (1619), Expect = 1.61e-226
Identity = 323/327 (98.78%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA    PAPPLSAVATE
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA----PAPPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGLVSTQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 323

BLAST of Cp4.1LG18g09280 vs. NCBI nr
Match: XP_022987155.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 627 bits (1618), Expect = 2.28e-226
Identity = 322/327 (98.47%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA    PAPPLSAVATE
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA----PAPPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 323

BLAST of Cp4.1LG18g09280 vs. NCBI nr
Match: XP_022922008.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 626 bits (1614), Expect = 9.30e-226
Identity = 322/327 (98.47%), Postives = 322/327 (98.47%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSAFPYPTLCFHPDSTTN RFFLSPPPLKLVKFSRTPNVNAPA    PAPPLSAVATE
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNPRFFLSPPPLKLVKFSRTPNVNAPA----PAPPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGLVSTQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 323

BLAST of Cp4.1LG18g09280 vs. NCBI nr
Match: XP_038880651.1 (magnesium protoporphyrin IX methyltransferase, chloroplastic [Benincasa hispida])

HSP 1 Score: 600 bits (1546), Expect = 2.00e-215
Identity = 309/327 (94.50%), Postives = 313/327 (95.72%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSA PYPTLCFHPDST+NSRFFLSP P+KLVKFSRT NVN PA      PPLSAVATE
Sbjct: 1   MAHSASPYPTLCFHPDSTSNSRFFLSPTPIKLVKFSRTLNVNVPA------PPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVVGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLA KRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVE ALR
Sbjct: 241 KADGMIAHLASLAGKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVEAALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 321

BLAST of Cp4.1LG18g09280 vs. NCBI nr
Match: XP_008464364.1 (PREDICTED: magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis melo] >ADN33868.1 magnesium-protoporphyrin O-methyltransferase [Cucumis melo subsp. melo] >KAA0057727.1 magnesium protoporphyrin IX methyltransferase [Cucumis melo var. makuwa] >TYJ98412.1 magnesium protoporphyrin IX methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 576 bits (1485), Expect = 3.28e-206
Identity = 297/327 (90.83%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           M+HSA  Y TLCFHPDST+NSRFFLSP P+KL+KFSRT N+N           +SAVATE
Sbjct: 1   MSHSASRYLTLCFHPDSTSNSRFFLSPTPIKLIKFSRTLNLN-----------VSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEGPL GVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GA VFASDISAAMVAEA+NKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of Cp4.1LG18g09280 vs. ExPASy TrEMBL
Match: A0A6J1JG16 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484791 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 1.11e-226
Identity = 322/327 (98.47%), Postives = 323/327 (98.78%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA    PAPPLSAVATE
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPA----PAPPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 323

BLAST of Cp4.1LG18g09280 vs. ExPASy TrEMBL
Match: A0A6J1E5D5 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430090 PE=4 SV=1)

HSP 1 Score: 626 bits (1614), Expect = 4.50e-226
Identity = 322/327 (98.47%), Postives = 322/327 (98.47%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           MAHSAFPYPTLCFHPDSTTN RFFLSPPPLKLVKFSRTPNVNAPA    PAPPLSAVATE
Sbjct: 1   MAHSAFPYPTLCFHPDSTTNPRFFLSPPPLKLVKFSRTPNVNAPA----PAPPLSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGLVSTQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 323

BLAST of Cp4.1LG18g09280 vs. ExPASy TrEMBL
Match: A0A5D3BF92 (Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00080 PE=4 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 1.59e-206
Identity = 297/327 (90.83%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           M+HSA  Y TLCFHPDST+NSRFFLSP P+KL+KFSRT N+N           +SAVATE
Sbjct: 1   MSHSASRYLTLCFHPDSTSNSRFFLSPTPIKLIKFSRTLNLN-----------VSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEGPL GVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GA VFASDISAAMVAEA+NKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of Cp4.1LG18g09280 vs. ExPASy TrEMBL
Match: E5GBL6 (Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 1.59e-206
Identity = 297/327 (90.83%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           M+HSA  Y TLCFHPDST+NSRFFLSP P+KL+KFSRT N+N           +SAVATE
Sbjct: 1   MSHSASRYLTLCFHPDSTSNSRFFLSPTPIKLIKFSRTLNLN-----------VSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEGPL GVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GA VFASDISAAMVAEA+NKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of Cp4.1LG18g09280 vs. ExPASy TrEMBL
Match: A0A1S3CMU6 (magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502274 PE=4 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 1.59e-206
Identity = 297/327 (90.83%), Postives = 307/327 (93.88%), Query Frame = 0

Query: 1   MAHSAFPYPTLCFHPDSTTNSRFFLSPPPLKLVKFSRTPNVNAPAPAPAPAPPLSAVATE 60
           M+HSA  Y TLCFHPDST+NSRFFLSP P+KL+KFSRT N+N           +SAVATE
Sbjct: 1   MSHSASRYLTLCFHPDSTSNSRFFLSPTPIKLIKFSRTLNLN-----------VSAVATE 60

Query: 61  LPVVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120
           LP+VDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW
Sbjct: 61  LPIVDGATIAAIGGGSVAALAAVLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRW 120

Query: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKE 180
           KKIYGETDDVNRVQRDIRLGHSKTVENVM MLKDEGPL GVTVCDAGCGTGSLSIPLAKE
Sbjct: 121 KKIYGETDDVNRVQRDIRLGHSKTVENVMAMLKDEGPLRGVTVCDAGCGTGSLSIPLAKE 180

Query: 181 GAIVFASDISAAMVAEAQNKAKEEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240
           GA VFASDISAAMVAEA+NKAKEEV GGV+PKFEVKDLESLEGKYDTVVCLDVLIHYPQN
Sbjct: 181 GAKVFASDISAAMVAEAENKAKEEVKGGVMPKFEVKDLESLEGKYDTVVCLDVLIHYPQN 240

Query: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300
           KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR
Sbjct: 241 KADGMIAHLASLAEKRLIISFAPKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALR 300

Query: 301 RVGWKIRKRGLVSTQFYFATLVEAVPA 327
           RVGWKIRKRGL+STQFYFATLVEAVPA
Sbjct: 301 RVGWKIRKRGLISTQFYFATLVEAVPA 316

BLAST of Cp4.1LG18g09280 vs. TAIR 10
Match: AT4G25080.3 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 429.5 bits (1103), Expect = 2.4e-120
Identity = 220/295 (74.58%), Postives = 251/295 (85.08%), Query Frame = 0

Query: 32  LVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPER 91
           L +F      N   P    A  ++A  T+L  VD  TIA +GGGSVAALAA++SLTDPER
Sbjct: 18  LPRFPNATRFNV-TPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAMVSLTDPER 77

Query: 92  RRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGM 151
           RR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+KTVEN M M
Sbjct: 78  RRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAKTVENTMLM 137

Query: 152 LKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVP 211
           L ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK ++    +P
Sbjct: 138 LTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKAQLPSENLP 197

Query: 212 KFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLL 271
           KFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAPKTFYYD+L
Sbjct: 198 KFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAPKTFYYDIL 257

Query: 272 KRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
           KR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+EAVP
Sbjct: 258 KRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIEAVP 311

BLAST of Cp4.1LG18g09280 vs. TAIR 10
Match: AT4G25080.1 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 429.5 bits (1103), Expect = 2.4e-120
Identity = 220/295 (74.58%), Postives = 251/295 (85.08%), Query Frame = 0

Query: 32  LVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPER 91
           L +F      N   P    A  ++A  T+L  VD  TIA +GGGSVAALAA++SLTDPER
Sbjct: 18  LPRFPNATRFNV-TPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAMVSLTDPER 77

Query: 92  RRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGM 151
           RR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+KTVEN M M
Sbjct: 78  RRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAKTVENTMLM 137

Query: 152 LKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVP 211
           L ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK ++    +P
Sbjct: 138 LTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKAQLPSENLP 197

Query: 212 KFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLL 271
           KFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAPKTFYYD+L
Sbjct: 198 KFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAPKTFYYDIL 257

Query: 272 KRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
           KR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+EAVP
Sbjct: 258 KRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIEAVP 311

BLAST of Cp4.1LG18g09280 vs. TAIR 10
Match: AT4G25080.2 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 429.5 bits (1103), Expect = 2.4e-120
Identity = 220/295 (74.58%), Postives = 251/295 (85.08%), Query Frame = 0

Query: 32  LVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPER 91
           L +F      N   P    A  ++A  T+L  VD  TIA +GGGSVAALAA++SLTDPER
Sbjct: 18  LPRFPNATRFNV-TPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAMVSLTDPER 77

Query: 92  RRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGM 151
           RR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+KTVEN M M
Sbjct: 78  RRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAKTVENTMLM 137

Query: 152 LKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVP 211
           L ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK ++    +P
Sbjct: 138 LTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKAQLPSENLP 197

Query: 212 KFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLL 271
           KFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAPKTFYYD+L
Sbjct: 198 KFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAPKTFYYDIL 257

Query: 272 KRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
           KR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+EAVP
Sbjct: 258 KRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIEAVP 311

BLAST of Cp4.1LG18g09280 vs. TAIR 10
Match: AT4G25080.5 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 429.5 bits (1103), Expect = 2.4e-120
Identity = 220/295 (74.58%), Postives = 251/295 (85.08%), Query Frame = 0

Query: 32  LVKFSRTPNVNAPAPAPAPAPPLSAVATELPVVDGATIAAIGGGSVAALAAVLSLTDPER 91
           L +F      N   P    A  ++A  T+L  VD  TIA +GGGSVAALAA++SLTDPER
Sbjct: 18  LPRFPNATRFNV-TPRSRAATVVAASVTDLAGVDSTTIAVLGGGSVAALAAMVSLTDPER 77

Query: 92  RRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHSKTVENVMGM 151
           RR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+KTVEN M M
Sbjct: 78  RRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHAKTVENTMLM 137

Query: 152 LKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAKEEVAGGVVP 211
           L ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK ++    +P
Sbjct: 138 LTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAKAQLPSENLP 197

Query: 212 KFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFAPKTFYYDLL 271
           KFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFAPKTFYYD+L
Sbjct: 198 KFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFAPKTFYYDIL 257

Query: 272 KRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLVEAVP 327
           KR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+EAVP
Sbjct: 258 KRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLIEAVP 311

BLAST of Cp4.1LG18g09280 vs. TAIR 10
Match: AT4G25080.4 (magnesium-protoporphyrin IX methyltransferase )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 197/244 (80.74%), Postives = 223/244 (91.39%), Query Frame = 0

Query: 83  VLSLTDPERRRRLQAEEVGGGDKDVVKEYFNNSGFQRWKKIYGETDDVNRVQRDIRLGHS 142
           ++SLTDPERRR+LQAEEVGGGDK+VV+EYFN++GF+RW+KIYGETD+VNRVQ+DIRLGH+
Sbjct: 1   MVSLTDPERRRKLQAEEVGGGDKEVVREYFNSTGFERWRKIYGETDEVNRVQKDIRLGHA 60

Query: 143 KTVENVMGMLKDEGPLTGVTVCDAGCGTGSLSIPLAKEGAIVFASDISAAMVAEAQNKAK 202
           KTVEN M ML ++  L GVTVCDAGCGTG LSIPLAKEGAIV ASDISAAMVAEA+ KAK
Sbjct: 61  KTVENTMLMLTEDRSLAGVTVCDAGCGTGLLSIPLAKEGAIVSASDISAAMVAEAEMKAK 120

Query: 203 EEVAGGVVPKFEVKDLESLEGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRLIISFA 262
            ++    +PKFEV DLESL GKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKR+I+SFA
Sbjct: 121 AQLPSENLPKFEVNDLESLTGKYDTVVCLDVLIHYPQNKADGMIAHLASLAEKRVILSFA 180

Query: 263 PKTFYYDLLKRVGELFPGPSKATRAYLHSEADVETALRRVGWKIRKRGLVSTQFYFATLV 322
           PKTFYYD+LKR+GELFPGPSKATRAYLHSEADVE AL +VGWKI KRGL +TQFYF+ L+
Sbjct: 181 PKTFYYDILKRIGELFPGPSKATRAYLHSEADVERALGKVGWKISKRGLTTTQFYFSRLI 240

Query: 323 EAVP 327
           EAVP
Sbjct: 241 EAVP 244

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SW183.4e-11974.58Magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Arabidopsis thal... [more]
Q554675.6e-6957.66Magnesium-protoporphyrin O-methyltransferase OS=Synechocystis sp. (strain PCC 68... [more]
P262364.5e-1828.64Magnesium-protoporphyrin O-methyltransferase OS=Rhodobacter capsulatus OX=1061 G... [more]
Q9PAM55.7e-0531.45Ubiquinone biosynthesis O-methyltransferase OS=Xylella fastidiosa (strain 9a5c) ... [more]
A7MPA97.4e-0532.00Ubiquinone biosynthesis O-methyltransferase OS=Cronobacter sakazakii (strain ATC... [more]
Match NameE-valueIdentityDescription
XP_023516229.11.61e-22698.78magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita pepo sub... [more]
XP_022987155.12.28e-22698.47magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita maxima][more]
XP_022922008.19.30e-22698.47magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucurbita moschata... [more]
XP_038880651.12.00e-21594.50magnesium protoporphyrin IX methyltransferase, chloroplastic [Benincasa hispida][more]
XP_008464364.13.28e-20690.83PREDICTED: magnesium protoporphyrin IX methyltransferase, chloroplastic [Cucumis... [more]
Match NameE-valueIdentityDescription
A0A6J1JG161.11e-22698.47magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita maxima... [more]
A0A6J1E5D54.50e-22698.47magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucurbita moscha... [more]
A0A5D3BF921.59e-20690.83Magnesium protoporphyrin IX methyltransferase OS=Cucumis melo var. makuwa OX=119... [more]
E5GBL61.59e-20690.83Magnesium-protoporphyrin O-methyltransferase OS=Cucumis melo subsp. melo OX=4126... [more]
A0A1S3CMU61.59e-20690.83magnesium protoporphyrin IX methyltransferase, chloroplastic OS=Cucumis melo OX=... [more]
Match NameE-valueIdentityDescription
AT4G25080.32.4e-12074.58magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.12.4e-12074.58magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.22.4e-12074.58magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.52.4e-12074.58magnesium-protoporphyrin IX methyltransferase [more]
AT4G25080.41.1e-11280.74magnesium-protoporphyrin IX methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010251Magnesium-protoporphyrin IX methyltransferaseTIGRFAMTIGR02021TIGR02021coord: 104..323
e-value: 1.1E-87
score: 291.1
IPR010251Magnesium-protoporphyrin IX methyltransferasePROSITEPS51556SAM_MT_MG_PIXcoord: 1..326
score: 110.844002
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 101..327
e-value: 6.8E-66
score: 223.9
NoneNo IPR availablePIRSRPIRSR000385-2PIRSR000385-2coord: 146..232
e-value: 4.0E-8
score: 30.7
NoneNo IPR availablePANTHERPTHR43591:SF32MAGNESIUM PROTOPORPHYRIN IX METHYLTRANSFERASE, CHLOROPLASTIC-RELATEDcoord: 69..323
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 69..323
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 162..261
e-value: 1.68434E-8
score: 49.7359
IPR010940Magnesium-protoporphyrin IX methyltransferase, C-terminalPFAMPF07109Mg-por_mtran_Ccoord: 230..325
e-value: 7.5E-35
score: 119.2
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 100..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g09280.1Cp4.1LG18g09280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034605 cellular response to heat
biological_process GO:0015995 chlorophyll biosynthetic process
biological_process GO:0032259 methylation
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0046406 magnesium protoporphyrin IX methyltransferase activity