Cp4.1LG18g08550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g08550
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCRAL-TRIO domain-containing protein C3H8.02
LocationCp4.1LG18: 7842511 .. 7846309 (-)
RNA-Seq ExpressionCp4.1LG18g08550
SyntenyCp4.1LG18g08550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGCGACTCCCCATCTCTACCTTCGCTGGCCTTCTTCTTTGCAACAGTTCATCTTCTATCCTGCTTAAGAAATGGCGGTACGTCTCAGTCTCACTCCACGCCACCCACTTCTCCCCACTACTTCTCCAATTCCACCCAGAGCTACTCGATTCCGCAGATTTGCAGTTCAGAATTCCGTCTCCGGCTCCGATGAATCGCGCAAGGTACTATGCTTCCACTCATTATCTACCATTTCTAGTTCGCGTCTGCCTCACATTGCCTTTCGAGAGACCTATTTTCCCTGAGAATACGATGGTGAAGTTTTGTATGATTGGATTCAAATTACGCCTTCGAACTTCTTTATGTTTGTGTCCGTGATTTGATGAATTTTGTGTCTGCCATCGTCTTCTTGTCACGAGGTTGCCTTAGAATTTAGCTGTAATGCGGTGCGAAAATTCTAGCATGCGAGCGTCTATCCCTTATCTGAGGAGAATTGTGTTTACTTTCTGTCAATCGTTTAATATGTGTAACTGGCAGTTGTTTGAAGATTGTGCGGCTTTACGTCTGATTGACTGGGTTGCCTATATGTTTATACGTATATGATGATGAACTCGATAGTTTATGGTCCATGTTTTCAAGTGTTGCAGCTCGTGTTGGAAGTGAAGGAGAAACTCGAAAGAGAACATTCTAGTCTTCCTCTTGGTCGGAATGGACGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGATCGAAAGTATTCTGTTGAAGATGCGGTTGCAAAATTAACGAAAGCAATTGTATACTCTTGAAACCTCACTTTTTTATTGTTGCATGTCTCTTGTTCAAGTGGCGTTACCAAAGCTGAATTTGCTGTATTAGTTGTTTTTCAAAATAAGACTAAGATAAAAGTATTAGAAAAACGGGTTCCATCTCAAAACCAATTCACTATAAGAGAAGTAACTCATGCATCTTACAAAGATAGTGAGGTTTCTTCATTTATGTGATCTTCACCAAAAAGTTTTGCATGGTTTATACTGAAATTTTAGTGAACGTTAGGAATGATATTGCCATGGAAGGCGTTGCATAATTACATGCATTTCATTGTCCCTTTGTCTCAAACAGAACTGGCGTCAGGAATTTGGAGTAGATGAACTGACTGAAAATTCAGTAAAGGGAATGGCTGAAACTGGGAAGGCATTCGTCCATGAGTTTCTTGATGTCAATCTCCGGCCGGTACTCATAGTTGTGGCTTCCAAGCATTTACCTGCAGTAAGTTACCTATTTATATTTGACAAATTTTCTTGGTTTGAGTCAATTAGCCAATCCCACCATTGTCCATAGGTCACTTTCAGTACAATTGTCTTCCTTTGTGATTGAGATTTACTCCTTTGTCATTGTAATTTGAAAGCTTATGCATTGTCTTGCAACTGAGAGAAACTCTGTTTTCTTATTGATCAAGAAGAAATCTCTCAGACATGTACAAATAAATTGAAAGAAATTTGCATAACAGCTGTAACTAAAATTCTTAGACAGCCGGAACTAATTTCAGTAAATTACAAAATTACAAAGGTAAATATCTAATAAACTGGCCATGAATCAATGATGCACCTATTCCACGAATGGTGAAGTTTGACATTATGACGTGGACTTTGACCTCTGTCCTTCCTCAGTGAACACTTGTTCTTATTTTTATTGTAAAATTATGTTTTTGTGTATAATCTTATTTGAATTTACTCTCTTAACATGAATTTTGACTTTCCTAATAACTTGACCGCAGGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGAAAAGAAGAAATACTTGGAATAATTGATCTGCGAGGATTTAGTATTGAGAATGCAGATCTTAAGTTCTTGACATTTTTGGTAATTTTACAAGCTGAAAATTTTCATTCTGAATTAGTGGGAACTCAGAAAGAATATTTGCAAGTTTGTGATGCGATTCATATCTTAACCACTTGGTACTAGCCGAACTAATGATCTGTACACAATTTACACTTTTTAGCATCACAAGTTTTTGCTCACTTTACCAGGAAGAAGCTCTTGCTAAAATAGTTGTGCTAGTCAATCATTGATTTTAAGACATGAAAATTATATTGCATTTTACTTTTCCACAAGACAACAACTAGTTGGTATGCGTACCAGAGTGCTAGCCTGCAGCTGCAAGGATGCTTCATTTAGTAATTAGCACTATAACTTAATTCATAGTGTGGAAAATGGGATTTTCTTCCCCCCATTCCCTTTTCCCCTTAGGGAGCCCTCTATGTTTTCAGTTTCTGGTGGGTTAGGTGTTTGGACGGGGCCAAGAGATTTTGTGTTTTGATGGCTTCTATGTCATTTGTCAGTTGTTCTTTCTGATCTCTTTGTGCTCTTTTTTTTCTGGCAGTTTGATGTGTTCTACTCCTACTATCCGAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCATCTGTGTTCAGACCACTATGGCAACTTACCAAGCCATTATTAAAATCATATGCCTCTCTGGTATGCATTTCCATTTCTAAACTGCAGATTCAGCTCTAGCCATAAATCTAGATTAATAAATATATTGGATGCAAAGTTGATCACTAATCTCCCTGTTCAATTCAAATTATAGATTCCTTCACTTGCATGTGGAGTTTGCTTTTCTTCTTTTGATTGTCAATGAAGAAATCTTGCATTTTCAAGTTTACTCCATCCGGCCACCAACCCTACACATCCTGAAGATGTTTCACCATTTTAGAAAACTACACATTAGGATTTGGTCACTTAAATTTTATTGAGCTGTTTGGAGTTGTTGCCTTCAGGCCCTTCCCTACATCCTATTTCTAATAGTTTCTGTAATCTTTTTCCTTTTTGAAGGCGAGATTTTGTTCCGTAGATACAGTCAGAAAGGAATACTTTACTGAAGCTACACTGCCTGCCATCTTTAGAAACTGAGGAATTTTGTCACTCTCGACAAACAGGTCCGAATTAGGTAGTATCAAGTCAGATGCTTACTGTAAAGATTATAAAATTCCACCTGTTCATAACAAGAAGAAGATTCTTTTAAATACATTTGATATTCACTTTGCTAATTGTTGGGGTTACATTTCTCCAGAGATGAAGGTCAATTAACGCTAATAGGCCCATGCAACTAATAAATTTCTGAGTTCAATATCGTTCCTCATGATTCTCAGAAATCTGATTGAAAACTAAAATGTATATTGGTATCTAATTTGGAATAGAGTTTACTTATTGACAATAGGAATTTTGCATATTCAGGTCCTTTGCTTGCTGCTTCTGCAATATGGTGGGGACGATCTGCTGAAGCAAATATTTCATACGAATGAAATTCTCCCCCCTCAAGTTCTATGTTCTGATTCGATACATGACGGGGAATTCAGAGATTTTCAAGTATGGGTTCAAAACTGGAGTATGGAGGTAAGCGATCCCACCTAAAGGAAGTTCAAACACGTAAATTTTTATTCTTGTATCATGTTAAATTGTCACCAAGTGTACTTGATTTTTTTGAGTAAAGAAAATAATCACATTTGCGTGCTCCCTCTAATTATCTAGATTTGTCCATTTGCAATTTTGTAAGGCTTCCCACATTGGCTAGATAAAGATAGATAAGGAAATTGTGGATACGGACAATAATTTTCATTGGCATGTGACCTTTTGGTGAAATTAAAAAGAGAAGTCATCAGCCCTTATGTTCAAATAGACAATGTTGCACAGTGGAGGATAGGTTTGTTGGGTATGGAGT

mRNA sequence

GGCGCGACTCCCCATCTCTACCTTCGCTGGCCTTCTTCTTTGCAACAGTTCATCTTCTATCCTGCTTAAGAAATGGCGGTACGTCTCAGTCTCACTCCACGCCACCCACTTCTCCCCACTACTTCTCCAATTCCACCCAGAGCTACTCGATTCCGCAGATTTGCAGTTCAGAATTCCGTCTCCGGCTCCGATGAATCGCGCAAGCTCGTGTTGGAAGTGAAGGAGAAACTCGAAAGAGAACATTCTAGTCTTCCTCTTGGTCGGAATGGACGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGATCGAAAGTATTCTGTTGAAGATGCGGTTGCAAAATTAACGAAAGCAATTAACTGGCGTCAGGAATTTGGAGTAGATGAACTGACTGAAAATTCAGTAAAGGGAATGGCTGAAACTGGGAAGGCATTCGTCCATGAGTTTCTTGATGTCAATCTCCGGCCGGTACTCATAGTTGTGGCTTCCAAGCATTTACCTGCAGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGAAAAGAAGAAATACTTGGAATAATTGATCTGCGAGGATTTAGTATTGAGAATGCAGATCTTAAGTTCTTGACATTTTTGTTTGATGTGTTCTACTCCTACTATCCGAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCATCTGTGTTCAGACCACTATGGCAACTTACCAAGCCATTATTAAAATCATATGCCTCTCTGGTCCTTTGCTTGCTGCTTCTGCAATATGGTGGGGACGATCTGCTGAAGCAAATATTTCATACGAATGAAATTCTCCCCCCTCAAGTTCTATGTTCTGATTCGATACATGACGGGGAATTCAGAGATTTTCAAGTATGGGTTCAAAACTGGAGTATGGAGGTAAGCGATCCCACCTAAAGGAAGTTCAAACACGTAAATTTTTATTCTTGTATCATGTTAAATTGTCACCAAGTGTACTTGATTTTTTTGAGTAAAGAAAATAATCACATTTGCGTGCTCCCTCTAATTATCTAGATTTGTCCATTTGCAATTTTGTAAGGCTTCCCACATTGGCTAGATAAAGATAGATAAGGAAATTGTGGATACGGACAATAATTTTCATTGGCATGTGACCTTTTGGTGAAATTAAAAAGAGAAGTCATCAGCCCTTATGTTCAAATAGACAATGTTGCACAGTGGAGGATAGGTTTGTTGGGTATGGAGT

Coding sequence (CDS)

ATGGCGGTACGTCTCAGTCTCACTCCACGCCACCCACTTCTCCCCACTACTTCTCCAATTCCACCCAGAGCTACTCGATTCCGCAGATTTGCAGTTCAGAATTCCGTCTCCGGCTCCGATGAATCGCGCAAGCTCGTGTTGGAAGTGAAGGAGAAACTCGAAAGAGAACATTCTAGTCTTCCTCTTGGTCGGAATGGACGGGACGATGAAGAGATGATCCTCTGGTTTCTGAAGGATCGAAAGTATTCTGTTGAAGATGCGGTTGCAAAATTAACGAAAGCAATTAACTGGCGTCAGGAATTTGGAGTAGATGAACTGACTGAAAATTCAGTAAAGGGAATGGCTGAAACTGGGAAGGCATTCGTCCATGAGTTTCTTGATGTCAATCTCCGGCCGGTACTCATAGTTGTGGCTTCCAAGCATTTACCTGCAGTCCATGATCCTGTTGAGGACGAGAAACTGTGTGTGTTCTATGTAGAGAAGGCACTGAGCAAGCTTCCACCTGGAAAAGAAGAAATACTTGGAATAATTGATCTGCGAGGATTTAGTATTGAGAATGCAGATCTTAAGTTCTTGACATTTTTGTTTGATGTGTTCTACTCCTACTATCCGAAACGGCTAGGTCAAGTCCTCTTTGTGGAAGCTCCATCTGTGTTCAGACCACTATGGCAACTTACCAAGCCATTATTAAAATCATATGCCTCTCTGGTCCTTTGCTTGCTGCTTCTGCAATATGGTGGGGACGATCTGCTGAAGCAAATATTTCATACGAATGAAATTCTCCCCCCTCAAGTTCTATGTTCTGATTCGATACATGACGGGGAATTCAGAGATTTTCAAGTATGGGTTCAAAACTGGAGTATGGAGGTAAGCGATCCCACCTAA

Protein sequence

MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILPPQVLCSDSIHDGEFRDFQVWVQNWSMEVSDPT
Homology
BLAST of Cp4.1LG18g08550 vs. ExPASy Swiss-Prot
Match: Q10138 (CRAL-TRIO domain-containing protein C3H8.02 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC3H8.02 PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 6.6e-13
Identity = 60/209 (28.71%), Postives = 99/209 (47.37%), Query Frame = 0

Query: 37  SGSDESRKLVLEVKEKLEREHSSLPL------GRNGRDDEEMILWFLKDRKYSVEDAVAK 96
           S +D+++ + LE  EK+  E     L        N  D + ++L FL+ RK++VE A+  
Sbjct: 85  SFNDKTKNVHLERVEKIASEWDPEGLRVCFWDAVNCDDPDGLLLRFLRARKWNVEAALEM 144

Query: 97  LTKAINWR-QEFGVDELT--------ENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKH 156
             K ++WR +E  V E+         ++        GK F+    D + RPV  + A  H
Sbjct: 145 FMKTVHWRSREMNVGEIVCNADHLDKDDDFVRQLRIGKCFIFG-EDKHNRPVCYIRARLH 204

Query: 157 LPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYS 216
                 P   E+L V+ +E A   L P  E    + D+  FS+ N D   L F+   F +
Sbjct: 205 KVGDVSPESVERLTVWVMETARLILKPPIETATVVFDMTDFSMSNMDYGPLKFMIKCFEA 264

Query: 217 YYPKRLGQVLFVEAPSVFRPLWQLTKPLL 231
           +YP+ LG+ +  +AP +F+ +W + K  L
Sbjct: 265 HYPECLGECIVHKAPWLFQGVWSIIKSWL 292

BLAST of Cp4.1LG18g08550 vs. ExPASy Swiss-Prot
Match: Q06705 (Phosphatidylinositol transfer protein CSR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=CSR1 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 9.6e-12
Identity = 51/168 (30.36%), Postives = 81/168 (48.21%), Query Frame = 0

Query: 73  ILWFLKDRKYSVEDAVAKLTKAINWRQ----------EFGVDELTENSVKGMAETGKAFV 132
           IL F++ RK++ +  +A L   + WR+          E  V E  E  V    E  KA +
Sbjct: 112 ILKFIRARKWNADKTIAMLGHDLYWRKDTINKIINGGERAVYENNETGVIKNLELQKATI 171

Query: 133 HEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKA---LSKLPPGKEEILGIIDL 192
             + D ++RPV++V    H  +     E EK  +  +E++     +  P    IL   DL
Sbjct: 172 QGY-DNDMRPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENYPASTTIL--FDL 231

Query: 193 RGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTK 228
            GFS+ N D   + FL   F ++YP+ LG +L  +AP +F P+W + K
Sbjct: 232 NGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIK 276

BLAST of Cp4.1LG18g08550 vs. ExPASy Swiss-Prot
Match: Q757H2 (Phosphatidylinositol transfer protein CSR1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=CSR1 PE=3 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 4.8e-11
Identity = 45/170 (26.47%), Postives = 78/170 (45.88%), Query Frame = 0

Query: 70  EEMILWFLKDRKYSVEDAVAKLTKAINWR------------QEFGVDELTENSVKGMAET 129
           + ++L F++ RK+ +  A+  +  ++ WR             E G  +  +  +    E 
Sbjct: 138 DNLLLRFVRARKWDINKAMIMMAHSLRWRLNEGKPEDIVFGGERGAQKADKKGIVKQLEL 197

Query: 130 GKAFVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGII 189
           GKA V  F D N  P++ V    H  A     E  +  +  +E+A   L    +    + 
Sbjct: 198 GKATVRGF-DKNGCPIVYVRPRLHHAADQTEAETSEYSLLIIEQARLFLKEPCDTATILF 257

Query: 190 DLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTK 228
           DL GFS+ N D   + FL   F ++YP+ LG++   +AP +F P+W + K
Sbjct: 258 DLSGFSMANMDYAPVKFLITCFEAHYPECLGKLFIHKAPWIFPPIWNIIK 306

BLAST of Cp4.1LG18g08550 vs. ExPASy Swiss-Prot
Match: Q9CWP6 (Motile sperm domain-containing protein 2 OS=Mus musculus OX=10090 GN=Mospd2 PE=1 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 4.0e-10
Identity = 53/237 (22.36%), Postives = 117/237 (49.37%), Query Frame = 0

Query: 37  SGSDESRKLVLEVKEKLEREHSSLPLGR-NGRD------DEEMILWFLKDRKYSVEDAVA 96
           + +    KL+ E + + E E+ +    + + RD      D+  +  +L  R   V++ + 
Sbjct: 4   NNAQNKAKLISETRRRFEAEYVTEKSEKYDSRDVERLQQDDNWVESYLYWRHNVVDETLK 63

Query: 97  KLTKAINWRQEFGVDELTENSV-KGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDP 156
            L ++  WR+EF V++L+E+S+ + + E G  ++H + D     +  +    H+      
Sbjct: 64  MLDESFQWRKEFSVNDLSESSIPRWLLELGGIYLHGY-DKEGNKLFWIRVKYHIKDQKTI 123

Query: 157 VEDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLG 216
           ++ +KL  F++E+  +K   GK  I  + D+    + + D+ F+ F+ + F  YYPK L 
Sbjct: 124 MDKKKLIAFWLER-YAKRENGK-PITVMFDMSETGLNSIDMDFVRFIINCFKVYYPKYLS 183

Query: 217 QVLFVEAPSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILPPQV 266
           +++  + P +    +++ K  L   A     + LL++   + +++ + + E LPP +
Sbjct: 184 KIVIFDMPWIMNAAFKIVKSWLGPEA-----VSLLKFTSKNEIQE-YVSVEYLPPHM 231

BLAST of Cp4.1LG18g08550 vs. ExPASy Swiss-Prot
Match: Q8NHP6 (Motile sperm domain-containing protein 2 OS=Homo sapiens OX=9606 GN=MOSPD2 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.4e-09
Identity = 54/230 (23.48%), Postives = 112/230 (48.70%), Query Frame = 0

Query: 44  KLVLEVKEKLEREHSSLPLGR-NGRD------DEEMILWFLKDRKYSVEDAVAKLTKAIN 103
           KL+ E + + E E+ +    + + RD      D+  +  +L  R   V++ +  L ++  
Sbjct: 11  KLISETRRRFEAEYVTDKSDKYDARDVERLQQDDNWVESYLSWRHNIVDETLKMLDESFQ 70

Query: 104 WRQEFGVDELTENSV-KGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLC 163
           WR+E  V++L E+S+ + + E G  ++H + D     +  +    H+      ++ +KL 
Sbjct: 71  WRKEISVNDLNESSIPRWLLEIGVIYLHGY-DKEGNKLFWIRVKYHVKDQKTILDKKKLI 130

Query: 164 VFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEA 223
            F++E+  +K   GK  +  + DL    I + D+ F+ F+ + F  YYPK L +++  + 
Sbjct: 131 AFWLER-YAKRENGK-PVTVMFDLSETGINSIDMDFVRFIINCFKVYYPKYLSKIVIFDM 190

Query: 224 PSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILPPQV 266
           P +    +++ K  L   A     + LL++   + + Q + + E LPP +
Sbjct: 191 PWLMNAAFKIVKTWLGPEA-----VSLLKFTSKNEV-QDYVSVEYLPPHM 231

BLAST of Cp4.1LG18g08550 vs. NCBI nr
Match: KAG6589438.1 (hypothetical protein SDJN03_14861, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 495 bits (1274), Expect = 1.36e-173
Identity = 256/281 (91.10%), Postives = 259/281 (92.17%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLTPR+PLLPTT PIPPRAT+FRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL
Sbjct: 1   MAVRLSLTPRYPLLPTTYPIPPRATQFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+HEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR
Sbjct: 121 FIHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASL---- 240
           GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASL    
Sbjct: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLARFC 240

Query: 241 ---------------VLCLLLLQYGGDDLLKQIFHTNEILP 262
                          VLCLLLLQYGGD LLKQIFHTNE LP
Sbjct: 241 SVDTKLRNFVTLDKQVLCLLLLQYGGDGLLKQIFHTNEFLP 281

BLAST of Cp4.1LG18g08550 vs. NCBI nr
Match: XP_023515523.1 (CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023515524.1 CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468 bits (1204), Expect = 7.63e-165
Identity = 240/262 (91.60%), Postives = 246/262 (93.89%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL
Sbjct: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR
Sbjct: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASL    
Sbjct: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLA--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +++ + T   LP
Sbjct: 241 ---RFCSVDTVRKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. NCBI nr
Match: XP_022921363.1 (CRAL-TRIO domain-containing protein C3H8.02-like isoform X2 [Cucurbita moschata] >KAG7023118.1 SPAC3H8.02, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461 bits (1187), Expect = 2.96e-162
Identity = 236/262 (90.08%), Postives = 245/262 (93.51%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLTPR+PLLPTT PIPPRAT+FRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL
Sbjct: 1   MAVRLSLTPRYPLLPTTYPIPPRATQFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+HEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR
Sbjct: 121 FIHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASL    
Sbjct: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLA--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +++ + T   LP
Sbjct: 241 ---RFCSVDTVRKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. NCBI nr
Match: XP_022921361.1 (CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita moschata] >XP_022921362.1 CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 445 bits (1145), Expect = 2.26e-155
Identity = 236/293 (80.55%), Postives = 245/293 (83.62%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRK---------------- 60
           MAVRLSLTPR+PLLPTT PIPPRAT+FRRFAVQNSVSGSDESRK                
Sbjct: 1   MAVRLSLTPRYPLLPTTYPIPPRATQFRRFAVQNSVSGSDESRKVPCFHSLSTISSSRLP 60

Query: 61  ---------------LVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA 120
                          LVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA
Sbjct: 61  HIACRERPIFPENAMLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA 120

Query: 121 KLTKAINWRQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDPV 180
           KLTKAINWRQEFGVDELTENSVKGMAETGKAF+HEFLDVNLRPVLIVVASKHLPAVHDPV
Sbjct: 121 KLTKAINWRQEFGVDELTENSVKGMAETGKAFIHEFLDVNLRPVLIVVASKHLPAVHDPV 180

Query: 181 EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ 240
           EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ
Sbjct: 181 EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ 240

Query: 241 VLFVEAPSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILP 262
           VLFVEAPSVFRPLWQLTKPLLKSYASL       ++   D +++ + T   LP
Sbjct: 241 VLFVEAPSVFRPLWQLTKPLLKSYASLA------RFCSVDTVRKEYFTEATLP 287

BLAST of Cp4.1LG18g08550 vs. NCBI nr
Match: XP_008464545.1 (PREDICTED: CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo] >KAA0057841.1 CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo var. makuwa] >TYJ98525.1 CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo var. makuwa])

HSP 1 Score: 430 bits (1106), Expect = 6.43e-150
Identity = 220/262 (83.97%), Postives = 237/262 (90.46%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLT RHPLLPT+ PIP RAT+FRRFAVQN VS SD+SRKLVLEVKEKLERE+SSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRK+SVEDAVAKLTKAINWRQEFGVDEL+E+SVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+H+FLDVN RPVL+VVASKHLP VHDPVEDEKLCVFYVEKALSKLPPGKEEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GF  ENADL+FLTFLFDVFY YYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV   
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +K+ + T   LP
Sbjct: 241 ---RFCSVDTIKKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. ExPASy TrEMBL
Match: A0A6J1E3Q4 (CRAL-TRIO domain-containing protein C3H8.02-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429653 PE=4 SV=1)

HSP 1 Score: 461 bits (1187), Expect = 1.44e-162
Identity = 236/262 (90.08%), Postives = 245/262 (93.51%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLTPR+PLLPTT PIPPRAT+FRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL
Sbjct: 1   MAVRLSLTPRYPLLPTTYPIPPRATQFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+HEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR
Sbjct: 121 FIHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASL    
Sbjct: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLA--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +++ + T   LP
Sbjct: 241 ---RFCSVDTVRKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. ExPASy TrEMBL
Match: A0A6J1E090 (CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429653 PE=4 SV=1)

HSP 1 Score: 445 bits (1145), Expect = 1.09e-155
Identity = 236/293 (80.55%), Postives = 245/293 (83.62%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRK---------------- 60
           MAVRLSLTPR+PLLPTT PIPPRAT+FRRFAVQNSVSGSDESRK                
Sbjct: 1   MAVRLSLTPRYPLLPTTYPIPPRATQFRRFAVQNSVSGSDESRKVPCFHSLSTISSSRLP 60

Query: 61  ---------------LVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA 120
                          LVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA
Sbjct: 61  HIACRERPIFPENAMLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVA 120

Query: 121 KLTKAINWRQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDPV 180
           KLTKAINWRQEFGVDELTENSVKGMAETGKAF+HEFLDVNLRPVLIVVASKHLPAVHDPV
Sbjct: 121 KLTKAINWRQEFGVDELTENSVKGMAETGKAFIHEFLDVNLRPVLIVVASKHLPAVHDPV 180

Query: 181 EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ 240
           EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ
Sbjct: 181 EDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQ 240

Query: 241 VLFVEAPSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILP 262
           VLFVEAPSVFRPLWQLTKPLLKSYASL       ++   D +++ + T   LP
Sbjct: 241 VLFVEAPSVFRPLWQLTKPLLKSYASLA------RFCSVDTVRKEYFTEATLP 287

BLAST of Cp4.1LG18g08550 vs. ExPASy TrEMBL
Match: A0A5A7UWI9 (CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001300 PE=4 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 3.11e-150
Identity = 220/262 (83.97%), Postives = 237/262 (90.46%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLT RHPLLPT+ PIP RAT+FRRFAVQN VS SD+SRKLVLEVKEKLERE+SSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRK+SVEDAVAKLTKAINWRQEFGVDEL+E+SVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+H+FLDVN RPVL+VVASKHLP VHDPVEDEKLCVFYVEKALSKLPPGKEEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GF  ENADL+FLTFLFDVFY YYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV   
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +K+ + T   LP
Sbjct: 241 ---RFCSVDTIKKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. ExPASy TrEMBL
Match: A0A1S3CLW3 (CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo OX=3656 GN=LOC103502396 PE=4 SV=1)

HSP 1 Score: 430 bits (1106), Expect = 3.11e-150
Identity = 220/262 (83.97%), Postives = 237/262 (90.46%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLT RHPLLPT+ PIP RAT+FRRFAVQN VS SD+SRKLVLEVKEKLERE+SSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRK+SVEDAVAKLTKAINWRQEFGVDEL+E+SVKGMAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDAVAKLTKAINWRQEFGVDELSEDSVKGMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+H+FLDVN RPVL+VVASKHLP VHDPVEDEKLCVFYVEKALSKLPPGKEEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLMVVASKHLPTVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GF  ENADL+FLTFLFDVFY YYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV   
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLV--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +K+ + T   LP
Sbjct: 241 ---RFCSVDTIKKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. ExPASy TrEMBL
Match: A0A0A0LS15 (CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004290 PE=4 SV=1)

HSP 1 Score: 422 bits (1084), Expect = 6.96e-147
Identity = 214/262 (81.68%), Postives = 235/262 (89.69%), Query Frame = 0

Query: 1   MAVRLSLTPRHPLLPTTSPIPPRATRFRRFAVQNSVSGSDESRKLVLEVKEKLEREHSSL 60
           MAVRLSLT RHPLLPT+ PIP RAT+FRRFAVQN VS SD+SRKLVLEVKEKLERE+SSL
Sbjct: 1   MAVRLSLTLRHPLLPTSYPIPVRATQFRRFAVQNCVSDSDDSRKLVLEVKEKLEREYSSL 60

Query: 61  PLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKA 120
           PLGRNGRDDEEMILWFLKDRK+SVED VAKLT+AINWR+EFGVDEL+E+ VK MAETGKA
Sbjct: 61  PLGRNGRDDEEMILWFLKDRKFSVEDTVAKLTRAINWRREFGVDELSEDKVKEMAETGKA 120

Query: 121 FVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLR 180
           F+H+FLDVN RPVL+VVASKHLPA+HDPVEDEKLCVFYVEKALSKLPPGKEEILGI+DLR
Sbjct: 121 FIHDFLDVNDRPVLLVVASKHLPAIHDPVEDEKLCVFYVEKALSKLPPGKEEILGIVDLR 180

Query: 181 GFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYASLVLCL 240
           GF  ENADL+FLTFLFDVFY YYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSY+SLV   
Sbjct: 181 GFRTENADLRFLTFLFDVFYFYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYSSLV--- 240

Query: 241 LLLQYGGDDLLKQIFHTNEILP 262
              ++   D +K+ + T   LP
Sbjct: 241 ---RFCSVDTIKKEYFTEATLP 256

BLAST of Cp4.1LG18g08550 vs. TAIR 10
Match: AT5G63060.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 299.7 bits (766), Expect = 2.6e-81
Identity = 144/234 (61.54%), Postives = 186/234 (79.49%), Query Frame = 0

Query: 29  RFAVQNSVSGSDESRKLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAV 88
           RF+V++ VS S  + KLVLEVKE+L ++ +SLPLG+ GRDDE+MILWFLKDR++SV++A+
Sbjct: 31  RFSVRSCVSESQHAHKLVLEVKERLAKDCTSLPLGKYGRDDEDMILWFLKDRRFSVDEAI 90

Query: 89  AKLTKAINWRQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDP 148
            KLTKAI WR EF VDEL+E+S+K   +TGKA+VH FLDV  RPV+IV  +KH+P + DP
Sbjct: 91  GKLTKAIKWRHEFKVDELSEDSIKAATDTGKAYVHGFLDVKGRPVVIVAPAKHIPGLLDP 150

Query: 149 VEDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLG 208
           +EDEKLCVF +EKALSKLP G+ +ILGI DLRGF  +NADLKFLTFLFDVFY YYP RL 
Sbjct: 151 IEDEKLCVFLLEKALSKLPAGQHKILGIFDLRGFGSQNADLKFLTFLFDVFYYYYPSRLD 210

Query: 209 QVLFVEAPSVFRPLWQLTKPLLKSYASLVLCLLLLQYGGDDLLKQIFHTNEILP 263
           +VLFV+AP +F+P+WQ TKPL+K YASLV      ++   + +++ + T E LP
Sbjct: 211 EVLFVDAPFIFQPIWQFTKPLVKQYASLV------KFCSAETVRKEYFTEETLP 258

BLAST of Cp4.1LG18g08550 vs. TAIR 10
Match: AT4G08690.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 92.0 bits (227), Expect = 8.3e-19
Identity = 57/193 (29.53%), Postives = 99/193 (51.30%), Query Frame = 0

Query: 40  DESRKLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQ 99
           +E RKL+  + EKL    S           ++ +L +L+ R + V+ A   L + + WR 
Sbjct: 24  EEVRKLLGPLPEKLSSFCS-----------DDAVLRYLRARNWHVKKATKMLKETLKWRV 83

Query: 100 EFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVAS-KHLPAVHDPVEDEKLCVFY 159
           ++  +E+    V G AETGK +    +D   RPVLI+  S ++  +V   +   +  V+ 
Sbjct: 84  QYKPEEICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQI---RYLVYC 143

Query: 160 VEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSV 219
           +E A+  LPPG+E+++ +ID  G+S+ N  L+       V   +YP+RL   +    P  
Sbjct: 144 MENAVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKF 202

Query: 220 FRPLWQLTKPLLK 232
           F P W++ +P L+
Sbjct: 204 FEPFWKVARPFLE 202

BLAST of Cp4.1LG18g08550 vs. TAIR 10
Match: AT4G08690.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 92.0 bits (227), Expect = 8.3e-19
Identity = 57/193 (29.53%), Postives = 99/193 (51.30%), Query Frame = 0

Query: 40  DESRKLVLEVKEKLEREHSSLPLGRNGRDDEEMILWFLKDRKYSVEDAVAKLTKAINWRQ 99
           +E RKL+  + EKL    S           ++ +L +L+ R + V+ A   L + + WR 
Sbjct: 24  EEVRKLLGPLPEKLSSFCS-----------DDAVLRYLRARNWHVKKATKMLKETLKWRV 83

Query: 100 EFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVAS-KHLPAVHDPVEDEKLCVFY 159
           ++  +E+    V G AETGK +    +D   RPVLI+  S ++  +V   +   +  V+ 
Sbjct: 84  QYKPEEICWEEVAGEAETGKIYRSSCVDKLGRPVLIMRPSVENSKSVKGQI---RYLVYC 143

Query: 160 VEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVEAPSV 219
           +E A+  LPPG+E+++ +ID  G+S+ N  L+       V   +YP+RL   +    P  
Sbjct: 144 MENAVQNLPPGEEQMVWMIDFHGYSLANVSLRTTKETAHVLQEHYPERLAFAVLYNPPKF 202

Query: 220 FRPLWQLTKPLLK 232
           F P W++ +P L+
Sbjct: 204 FEPFWKVARPFLE 202

BLAST of Cp4.1LG18g08550 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-13
Identity = 48/196 (24.49%), Postives = 89/196 (45.41%), Query Frame = 0

Query: 43  RKLVLEVKEKLEREHSSLPLGRNGRDDEE-------MILWFLKDRKYSVEDAVAKLTKAI 102
           +K V  +++++ER    +      R D E       MI  FL+ R   +E A       +
Sbjct: 16  QKTVPLIEDEIERSKVGIMRALCDRQDPETKEVDDLMIRRFLRARDLDIEKASTMFLNYL 75

Query: 103 NW-RQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLIVVASKHLPAVHDPVEDEKL 162
            W R       + E  +       K  +    D   RP+ + + ++H P+  +P E ++ 
Sbjct: 76  TWKRSMLPKGHIPEAEIANDLSHNKMCMQGH-DKMGRPIAVAIGNRHNPSKGNPDEFKRF 135

Query: 163 CVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTFLFDVFYSYYPKRLGQVLFVE 222
            V+ +EK  +++P G+E+ + I DL+G+   N D++            YP+RLG++  V 
Sbjct: 136 VVYTLEKICARMPRGQEKFVAIGDLQGWGYSNCDIRGYLAALSTLQDCYPERLGKLYIVH 195

Query: 223 APSVFRPLWQLTKPLL 231
           AP +F   W++  P +
Sbjct: 196 APYIFMTAWKVIYPFI 210

BLAST of Cp4.1LG18g08550 vs. TAIR 10
Match: AT1G22180.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.2e-12
Identity = 42/161 (26.09%), Postives = 79/161 (49.07%), Query Frame = 0

Query: 76  FLKDRKYSVEDAVAKLTKAINWRQEFGVDELTENSVKGMAETGKAFVHEFLDVNLRPVLI 135
           +L  R   V+ A   L + + WR ++  +E+    +   AETGK +     D   R VL+
Sbjct: 52  YLAARNGHVKKATKMLKETLKWRAQYKPEEIRWEEIAREAETGKIYRANCTDKYGRTVLV 111

Query: 136 VVAS-KHLPAVHDPVEDEKLCVFYVEKALSKLPPGKEEILGIIDLRGFSIENADLKFLTF 195
           +  S ++  +    +   ++ V+ +E A+  LP  +E+++ +ID  GF++ +  LK    
Sbjct: 112 MRPSCQNTKSYKGQI---RILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRE 171

Query: 196 LFDVFYSYYPKRLGQVLFVEAPSVFRPLWQLTKPLLKSYAS 236
              V   +YP+RLG  +    P +F   +++ KP L+   S
Sbjct: 172 TAHVLQEHYPERLGLAIVYNPPKIFESFYKMVKPFLEPKTS 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q101386.6e-1328.71CRAL-TRIO domain-containing protein C3H8.02 OS=Schizosaccharomyces pombe (strain... [more]
Q067059.6e-1230.36Phosphatidylinositol transfer protein CSR1 OS=Saccharomyces cerevisiae (strain A... [more]
Q757H24.8e-1126.47Phosphatidylinositol transfer protein CSR1 OS=Ashbya gossypii (strain ATCC 10895... [more]
Q9CWP64.0e-1022.36Motile sperm domain-containing protein 2 OS=Mus musculus OX=10090 GN=Mospd2 PE=1... [more]
Q8NHP63.4e-0923.48Motile sperm domain-containing protein 2 OS=Homo sapiens OX=9606 GN=MOSPD2 PE=1 ... [more]
Match NameE-valueIdentityDescription
KAG6589438.11.36e-17391.10hypothetical protein SDJN03_14861, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023515523.17.63e-16591.60CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita pepo subs... [more]
XP_022921363.12.96e-16290.08CRAL-TRIO domain-containing protein C3H8.02-like isoform X2 [Cucurbita moschata]... [more]
XP_022921361.12.26e-15580.55CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 [Cucurbita moschata]... [more]
XP_008464545.16.43e-15083.97PREDICTED: CRAL-TRIO domain-containing protein C3H8.02 [Cucumis melo] >KAA005784... [more]
Match NameE-valueIdentityDescription
A0A6J1E3Q41.44e-16290.08CRAL-TRIO domain-containing protein C3H8.02-like isoform X2 OS=Cucurbita moschat... [more]
A0A6J1E0901.09e-15580.55CRAL-TRIO domain-containing protein C3H8.02-like isoform X1 OS=Cucurbita moschat... [more]
A0A5A7UWI93.11e-15083.97CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CLW33.11e-15083.97CRAL-TRIO domain-containing protein C3H8.02 OS=Cucumis melo OX=3656 GN=LOC103502... [more]
A0A0A0LS156.96e-14781.68CRAL-TRIO domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G004290 P... [more]
Match NameE-valueIdentityDescription
AT5G63060.12.6e-8161.54Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.28.3e-1929.53Sec14p-like phosphatidylinositol transfer family protein [more]
AT4G08690.18.3e-1929.53Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G01630.11.1e-1324.49Sec14p-like phosphatidylinositol transfer family protein [more]
AT1G22180.21.2e-1226.09Sec14p-like phosphatidylinositol transfer family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 114..259
e-value: 2.3E-17
score: 73.7
IPR001251CRAL-TRIO lipid binding domainPFAMPF00650CRAL_TRIOcoord: 113..232
e-value: 2.8E-20
score: 72.6
IPR001251CRAL-TRIO lipid binding domainPROSITEPS50191CRAL_TRIOcoord: 110..265
score: 13.862279
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 131..232
e-value: 7.31796E-19
score: 79.7366
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 25..234
e-value: 1.8E-39
score: 137.6
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 111..232
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR47556SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEINcoord: 17..238
IPR036273CRAL/TRIO, N-terminal domain superfamilySUPERFAMILY46938CRAL/TRIO N-terminal domaincoord: 41..109

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g08550.1Cp4.1LG18g08550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0120009 intermembrane lipid transfer
biological_process GO:0015914 phospholipid transport
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0016020 membrane
molecular_function GO:0070300 phosphatidic acid binding
molecular_function GO:1901981 phosphatidylinositol phosphate binding
molecular_function GO:0008526 phosphatidylinositol transfer activity