Cp4.1LG18g07290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g07290
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCysteine dioxygenase
LocationCp4.1LG18: 7146286 .. 7149131 (+)
RNA-Seq ExpressionCp4.1LG18g07290
SyntenyCp4.1LG18g07290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATTCATAATCTCATTCCAAATTTGCATTTATTTTCTGTAAAAAAACTCCAAAATGTTCTCAAAATTCCAAAAAGCGTCGGATAAGATCAGGCTAATTTTGCAAAACAAGGTCGATTGCTCAGTGTTCGTTCGATCCTTGAGCATGAACCACAGGCAGAGCTCCAAAGTTCAGGCTATCTACGACCTCTGTAAGACCTCCTTAACTCCCACCAGCTCTCCACTGTCTTCTCAAACTATCCAGAATCTATGTTCCCTTTTGGGTAATTTCTCCGCCTTGCTTTGAAATTTATACTGCATTCTGTTCTACGTTGATCTTCAAACGACACTCTTTTGTTTCCTTTTGTGATTCTTGCTCAATTTCTCTTCTGGGGTTCAAAATTCAAGTAATTCAGTGCTGGGTTTGCATGAGATTGATGGATTGAATCATTGGTTTGTTCGTCTCAATTCATTTGAGAGTGTTACTCTCTCTCTTTTCTTTATTAAATTTTGGAGATCACTATGCCATTTGGGAATGCTTGGTTTACTCATGTGCTTTAATTGCTTCTGGCTTGGATATGTTGTTTAATGATTTGGATATAACTTCACAAGCTGTGAAATGAACATGTTTGTCTCTTGATTTCCACATGAGCAATGGCATTTGATGCCATTAATTTGTGATTTGTAGCCACAACTTTCTTCAGTTAAAAGGCTAACAGTGGTTTAGAATTTTTTATGTTGCCATATCTGCAATTCCTGGTACAATTGCAAACAATGATGATTGGAGAATCAATTATTATGATGTTCCTTTGCGTGTTTTCTGAAACTAGCCCAGGCTGCTATGCTAAAAAACATTGTTGGTTAGAACGCTGAAAGTCTTCAAGTAGGAAATATCACATTATGTGCTGCATTAGATTCCACTCTTTGACACGTCCCAGTTGTATGCTCCATTCACAGACACGGTTAGCCCCGGGGATTTTGGGCTTAAAGAAGAAAATATTGGTGACGACCGAGGGCATGGACCGTTTGGACTGAGCCAATTCAATCGGATAGCTAGATGGGCTCAACCAATAACATATATTGATATATTTGAAGGTGAAAGCTTTACTGTAAGTCCCATTCATCTCTTTTCTCAAACAGTCACATCCATTCATTGTTCAGTACTCAGAAATTTGGAATCCGAGAAATTTTAAGTTTAATTTCTAAGGAAATGATTACATTTTGAAGCTTCCATTCTAGGAGCTAGTTGTGTAATCGGATGGCAAAATCCATTATTTGGAGAAGTTTGTGTCAAAATTACCAGCAACAGTATCTTTGATTGACTGAAACTAGTCATGGGTTTCGGTTGGTTGTTTTCGAAGCCTTTGCTGTGATAATTATGCTTATGAGTGTACTTAATATCACGACACAGATATGTATATTCTGCTTTCCTACTTCTTCTGTCATCCCCCTTCATGACCATCCTGGGATGACTGTGTTTAGCAAAGTTCTCTATGGTTCTCTCCATGTGAAAGCCTATGACTGGGTTGAACCTGCCTTCATTCAGGAAAGTAAGGACTCCACCAATTTTCCAGGTTTGATTTCCAATACCAACTTGTTTTCTGGATGTACAGAAACAATCAACGAATAGTAATTTATCATTTTGAGTTTTGGTTTCGTAATGTTTTACTAGATTATAAATATTTTTCGCTGTTAGTGTAAAATGAACTTTTGCTAGATAGGAATGTCACTGCATAACATTATGAACACTCTGAGTGTACAATGTTCAACCATGTCCATGAGTCCATGACTATTGTATATCAGTAAGGGCAATTGTAGATTATTGTGCAGTTGACTGAATCTATGCACCGATTATCGTTGACGTAAAGATACCATTGAGTCATCTTGGCGTTTGGCTATTACCTTCTGAATGCAACTCTCTCTTCTCCTTTGTTTTTGGCTGAAACAACTGTTGCAACAATTTTTTAGTATGTTACCGATGAGCTGGTTGTGTATGCTGGCTTGCATTTCCCCTGTGGGATATCAGTTTATAGGTCATGAATTTATATACTACTAGAGATTGATACCAAGAGTTTCATCTACATTGTTAGGCACTAAGAGGATGCATCCTTTATCTTTTTGAATGCAAATCATTGGATTTCCTCTCATGAATTCCACCCACGCTCCCAACATTTTTTTCCAGGAAGATTAGCTAAGTTATCAGTTGATAAAGTTCTAACAGCATCAAGTGGAACATCCGTTTTGTACCCGAAGACCGGAGGTAATCTGCATTGTTTCACTGCAGTTTCGCCTTGTGCCGTGCTCGATGTTCTCTCGCCTCCTTATAACGAAGATAAAGGAAGAAGATGTACATACTTCCATGACTATCCTTATTCAACTTTTTGTAAGTAACCTTTTCTCCTTTATGTTTCTTGAATTTTCTTATTCAATCAACAAATCTTAGACACAGTGGCTCTGCAAAAAATCAGCTGCAGAATCCAAACCGAACCTAAACGATGAAGAAGTAGAAGATTATGCCTGGCTTGCAAAAATCGAGACCCCTGATGATCTATACATGCGTCCGGGGGTGTACGCTGGTCCTGCTATTCAAATATAGGCGTTGACAGATTTAGGATTATGGAGATTCTTCCAACGTCGACCAGGAAAGCTACTTCTACCTGGGATTATACACTAGATTTAGCTTTTGAGAGGAGGTTGATGTTTTTGTCCTTTATGCTGAGTTTACACAGTATTGATGACAGAATTGTAAGGTAAAAGCTGCACCCAGTTGCTTGCTGTTTTATCTTGGAAACTAATAAACATAGATTACTGTTAGTTGTTAGATTCGAATCGTCTCAAGTTAACTAGCAAAGTAGCAATATTTTTCATC

mRNA sequence

TATTCATAATCTCATTCCAAATTTGCATTTATTTTCTGTAAAAAAACTCCAAAATGTTCTCAAAATTCCAAAAAGCGTCGGATAAGATCAGGCTAATTTTGCAAAACAAGGTCGATTGCTCAGTGTTCGTTCGATCCTTGAGCATGAACCACAGGCAGAGCTCCAAAGTTCAGGCTATCTACGACCTCTGTAAGACCTCCTTAACTCCCACCAGCTCTCCACTGTCTTCTCAAACTATCCAGAATCTATGTTCCCTTTTGGTGCTGGGTTTGCATGAGATTGATGGATTGAATCATTGGTTTTTGTATGCTCCATTCACAGACACGGTTAGCCCCGGGGATTTTGGGCTTAAAGAAGAAAATATTGGTGACGACCGAGGGCATGGACCGTTTGGACTGAGCCAATTCAATCGGATAGCTAGATGGGCTCAACCAATAACATATATTGATATATTTGAAGGTGAAAGCTTTACTATATGTATATTCTGCTTTCCTACTTCTTCTGTCATCCCCCTTCATGACCATCCTGGGATGACTGTGTTTAGCAAAGTTCTCTATGGTTCTCTCCATGTGAAAGCCTATGACTGGGTTGAACCTGCCTTCATTCAGGAAAGTAAGGACTCCACCAATTTTCCAGGAAGATTAGCTAAGTTATCAGTTGATAAAGTTCTAACAGCATCAAGTGGAACATCCGTTTTGTACCCGAAGACCGGAGGTAATCTGCATTGTTTCACTGCAGTTTCGCCTTGTGCCGTGCTCGATGTTCTCTCGCCTCCTTATAACGAAGATAAAGGAAGAAGATGTACATACTTCCATGACTATCCTTATTCAACTTTTTCTGCAGAATCCAAACCGAACCTAAACGATGAAGAAGTAGAAGATTATGCCTGGCTTGCAAAAATCGAGACCCCTGATGATCTATACATGCGTCCGGGGGTGTACGCTGGTCCTGCTATTCAAATATAGGCGTTGACAGATTTAGGATTATGGAGATTCTTCCAACGTCGACCAGGAAAGCTACTTCTACCTGGGATTATACACTAGATTTAGCTTTTGAGAGGAGGTTGATGTTTTTGTCCTTTATGCTGAGTTTACACAGTATTGATGACAGAATTGTAAGGTAAAAGCTGCACCCAGTTGCTTGCTGTTTTATCTTGGAAACTAATAAACATAGATTACTGTTAGTTGTTAGATTCGAATCGTCTCAAGTTAACTAGCAAAGTAGCAATATTTTTCATC

Coding sequence (CDS)

ATGTTCTCAAAATTCCAAAAAGCGTCGGATAAGATCAGGCTAATTTTGCAAAACAAGGTCGATTGCTCAGTGTTCGTTCGATCCTTGAGCATGAACCACAGGCAGAGCTCCAAAGTTCAGGCTATCTACGACCTCTGTAAGACCTCCTTAACTCCCACCAGCTCTCCACTGTCTTCTCAAACTATCCAGAATCTATGTTCCCTTTTGGTGCTGGGTTTGCATGAGATTGATGGATTGAATCATTGGTTTTTGTATGCTCCATTCACAGACACGGTTAGCCCCGGGGATTTTGGGCTTAAAGAAGAAAATATTGGTGACGACCGAGGGCATGGACCGTTTGGACTGAGCCAATTCAATCGGATAGCTAGATGGGCTCAACCAATAACATATATTGATATATTTGAAGGTGAAAGCTTTACTATATGTATATTCTGCTTTCCTACTTCTTCTGTCATCCCCCTTCATGACCATCCTGGGATGACTGTGTTTAGCAAAGTTCTCTATGGTTCTCTCCATGTGAAAGCCTATGACTGGGTTGAACCTGCCTTCATTCAGGAAAGTAAGGACTCCACCAATTTTCCAGGAAGATTAGCTAAGTTATCAGTTGATAAAGTTCTAACAGCATCAAGTGGAACATCCGTTTTGTACCCGAAGACCGGAGGTAATCTGCATTGTTTCACTGCAGTTTCGCCTTGTGCCGTGCTCGATGTTCTCTCGCCTCCTTATAACGAAGATAAAGGAAGAAGATGTACATACTTCCATGACTATCCTTATTCAACTTTTTCTGCAGAATCCAAACCGAACCTAAACGATGAAGAAGTAGAAGATTATGCCTGGCTTGCAAAAATCGAGACCCCTGATGATCTATACATGCGTCCGGGGGTGTACGCTGGTCCTGCTATTCAAATATAG

Protein sequence

MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQTIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPAIQI
Homology
BLAST of Cp4.1LG18g07290 vs. ExPASy Swiss-Prot
Match: Q1G3U6 (Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 3.8e-88
Identity = 166/306 (54.25%), Postives = 206/306 (67.32%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           M S+  KA +K+   L +K D    +   S N  +S KVQ +YDLCK + T  +   +S 
Sbjct: 1   MLSRLFKAGEKVLSNLVSKKD----IYMASRNQEKSPKVQELYDLCKETFTGKAPSPASM 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
            IQ LCS+L                    D+VSP D GL+E +  DDRG+G  G+S+FNR
Sbjct: 61  AIQKLCSVL--------------------DSVSPADVGLEEVSQDDDRGYGVSGVSRFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           + RWAQPIT++DI E ++FT+CIFCFPTSSVIPLHDHP M VFSK+LYGSLHVKAYDWVE
Sbjct: 121 VGRWAQPITFLDIHECDTFTMCIFCFPTSSVIPLHDHPEMAVFSKILYGSLHVKAYDWVE 180

Query: 181 PAFI--QESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVL 240
           P  I  Q+     + P RLAKL  DKV+T  S    LYPKTGGNLHCFTA++PCAVLD+L
Sbjct: 181 PPCIITQDKGVPGSLPARLAKLVSDKVITPQSEIPALYPKTGGNLHCFTALTPCAVLDIL 240

Query: 241 SPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVED-YAWLAKIETPDDLYMRPGVYA 300
           SPPY E  GR C+Y+ DYP+STF+ E+     DE  ED YAWL +I+TPDDL+MRPG Y 
Sbjct: 241 SPPYKESVGRSCSYYMDYPFSTFALENGMKKVDEGKEDEYAWLVQIDTPDDLHMRPGSYT 282

Query: 301 GPAIQI 304
           GP I++
Sbjct: 301 GPTIRV 282

BLAST of Cp4.1LG18g07290 vs. ExPASy Swiss-Prot
Match: Q8LGJ5 (Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.0e-48
Identity = 99/215 (46.05%), Postives = 134/215 (62.33%), Query Frame = 0

Query: 90  DTVSPGDFGLKEENIGDDRGHGPFGLSQFNRIARWAQP-ITYIDIFEGESFTICIFCFPT 149
           D + P D G+  +            +S F        P +TY+ I+    F+ICIFC P 
Sbjct: 80  DEIKPEDVGVNPK------------MSYFRSTVTGRSPLVTYLHIYACHRFSICIFCLPP 139

Query: 150 SSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTA 209
           S VIPLH+HP MTVFSK+L+G++H+K+YDWV P   Q S D+     RLAK+ VD   TA
Sbjct: 140 SGVIPLHNHPEMTVFSKLLFGTMHIKSYDWV-PDSPQPSSDT-----RLAKVKVDSDFTA 199

Query: 210 SSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPN 269
              TS+LYP  GGN+HCFTA + CAVLDV+ PPY++  GR CTY+ DYP+S+FS +    
Sbjct: 200 PCDTSILYPADGGNMHCFTAKTACAVLDVIGPPYSDPAGRHCTYYFDYPFSSFSVDGVV- 259

Query: 270 LNDEEVEDYAWL-AKIETPDDLYMRPGVYAGPAIQ 303
           + +EE E YAWL  + E P+DL +   +Y+GP I+
Sbjct: 260 VAEEEKEGYAWLKEREEKPEDLTVTALMYSGPTIK 275

BLAST of Cp4.1LG18g07290 vs. ExPASy Swiss-Prot
Match: Q9LXG9 (Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-45
Identity = 91/184 (49.46%), Postives = 124/184 (67.39%), Query Frame = 0

Query: 122 ARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEP 181
           AR + PITY+ + + + F+I IFC P S VIPLH+HPGMTVFSK+L+G++H+K+YDWV  
Sbjct: 115 ARSSPPITYLHLHQCDQFSIGIFCLPPSGVIPLHNHPGMTVFSKLLFGTMHIKSYDWVVD 174

Query: 182 AFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPP 241
           A +++SK       RLAKL VD   TA    S+LYP+ GGN+H FTA++ CAVLDVL PP
Sbjct: 175 APMRDSKT------RLAKLKVDSTFTAPCNASILYPEDGGNMHRFTAITACAVLDVLGPP 234

Query: 242 YNEDKGRRCTYFHDYPYSTFSAESKPNL-NDEEVEDYAWL-AKIETPDDLYMRPG-VYAG 301
           Y   +GR CTYF ++P    S+E    L ++EE E YAWL  + + P+D     G +Y G
Sbjct: 235 YCNPEGRHCTYFLEFPLDKLSSEDDDVLSSEEEKEGYAWLQERDDNPEDHTNVVGALYRG 292

Query: 302 PAIQ 303
           P ++
Sbjct: 295 PKVE 292

BLAST of Cp4.1LG18g07290 vs. ExPASy Swiss-Prot
Match: Q9SJI9 (Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2)

HSP 1 Score: 182.2 bits (461), Expect = 8.9e-45
Identity = 95/263 (36.12%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 40  QAIYDLCKTSLTPTSSPLSSQTIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGL 99
           Q +Y+ CK S + +  P++   ++ + ++L                    + + P D G+
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVL--------------------EKIKPSDVGI 65

Query: 100 KEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPG 159
           +++        GP  L++ N   +    I Y+ + E +SF+I IFC P SS+IPLH+HPG
Sbjct: 66  EQDAQLARSRSGP--LNERNGSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPG 125

Query: 160 MTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKT 219
           MTV SK++YGS+HVK+YDW+EP   +    S   P +L K   D  +TA S  + LYPK+
Sbjct: 126 MTVLSKLVYGSMHVKSYDWLEPQLTEPEDPSQARPAKLVK---DTEMTAQSPVTTLYPKS 185

Query: 220 GGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAW 279
           GGN+HCF A++ CA+LD+L+PPY+ +  R CTYF          E +  ++ E V D  W
Sbjct: 186 GGNIHCFKAITHCAILDILAPPYSSEHDRHCTYFRKSRREDLPGELE--VDGEVVTDVTW 240

Query: 280 LAKIETPDDLYMRPGVYAGPAIQ 303
           L + + PDD  +R   Y GP I+
Sbjct: 246 LEEFQPPDDFVIRRIPYRGPVIR 240

BLAST of Cp4.1LG18g07290 vs. ExPASy Swiss-Prot
Match: Q9LXT4 (Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.2e-43
Identity = 96/264 (36.36%), Postives = 144/264 (54.55%), Query Frame = 0

Query: 39  VQAIYDLCKTSLTPTSSPLSSQTIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFG 98
           +Q +++ CK+SL+P + P+S + +  + ++L                    + + P D G
Sbjct: 5   IQRLFNTCKSSLSP-NGPVSEEALDKVRNVL--------------------EKIKPSDVG 64

Query: 99  LKEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHP 158
           L++E        GP      N  +  A  I Y+ + E +SF+I IFC P  S+IPLH+HP
Sbjct: 65  LEQEAQLVRNWPGPGNERNGNHHSLPA--IKYLQLHECDSFSIGIFCMPPGSIIPLHNHP 124

Query: 159 GMTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPK 218
           GMTV SK++YGS+HVK+YDW EP    +S+       R AKL  D  +T+ S  + LYP 
Sbjct: 125 GMTVLSKLVYGSMHVKSYDWAEP---DQSELDDPLQARPAKLVKDIDMTSPSPATTLYPT 184

Query: 219 TGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYA 278
           TGGN+HCF A++ CA+ D+LSPPY+   GR C YF   P      E +  +N E + +  
Sbjct: 185 TGGNIHCFKAITHCAIFDILSPPYSSTHGRHCNYFRKSPMLDLPGEIEV-MNGEVISNVT 241

Query: 279 WLAKIETPDDLYMRPGVYAGPAIQ 303
           WL + + PD+  +    Y GP I+
Sbjct: 245 WLEEYQPPDNFVIWRVPYRGPVIR 241

BLAST of Cp4.1LG18g07290 vs. NCBI nr
Match: XP_023516979.1 (plant cysteine oxidase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 574 bits (1479), Expect = 3.02e-206
Identity = 283/303 (93.40%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSPGDFGLKEENIGDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 283

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 283

BLAST of Cp4.1LG18g07290 vs. NCBI nr
Match: XP_022921368.1 (plant cysteine oxidase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 571 bits (1472), Expect = 3.53e-205
Identity = 280/303 (92.41%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSPGDFGLKEEN+GDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPGDFGLKEENMGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE+KDSTNFPGRLAKLSVDKVLTASSGTS+LYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQENKDSTNFPGRLAKLSVDKVLTASSGTSILYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 283

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 283

BLAST of Cp4.1LG18g07290 vs. NCBI nr
Match: XP_022988474.1 (plant cysteine oxidase 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 552 bits (1422), Expect = 1.48e-197
Identity = 272/303 (89.77%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQ+ASDKIRLILQNKVDCSVFVRS SMNHR+SSKVQA+Y LCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQRASDKIRLILQNKVDCSVFVRSFSMNHRKSSKVQALYHLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSP DFGLKEENIGDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPEDFGLKEENIGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIF FPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFSFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQESKDSTNF GRLAKL+VD+VLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQESKDSTNFQGRLAKLAVDEVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVY GPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYTGPA 283

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 283

BLAST of Cp4.1LG18g07290 vs. NCBI nr
Match: XP_022921369.1 (plant cysteine oxidase 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 546 bits (1406), Expect = 3.02e-195
Identity = 272/303 (89.77%), Postives = 274/303 (90.43%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSPGDFGLKEEN+GDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPGDFGLKEENMGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE         RLAKLSVDKVLTASSGTS+LYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQER--------RLAKLSVDKVLTASSGTSILYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 275

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 275

BLAST of Cp4.1LG18g07290 vs. NCBI nr
Match: XP_022988475.1 (plant cysteine oxidase 3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 528 bits (1361), Expect = 2.18e-188
Identity = 264/303 (87.13%), Postives = 269/303 (88.78%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQ+ASDKIRLILQNKVDCSVFVRS SMNHR+SSKVQA+Y LCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQRASDKIRLILQNKVDCSVFVRSFSMNHRKSSKVQALYHLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSP DFGLKEENIGDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPEDFGLKEENIGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIF FPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFSFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE         RLAKL+VD+VLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQER--------RLAKLAVDEVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVY GPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYTGPA 275

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 275

BLAST of Cp4.1LG18g07290 vs. ExPASy TrEMBL
Match: A0A6J1E3Q9 (Cysteine dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111429658 PE=3 SV=1)

HSP 1 Score: 571 bits (1472), Expect = 1.71e-205
Identity = 280/303 (92.41%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSPGDFGLKEEN+GDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPGDFGLKEENMGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE+KDSTNFPGRLAKLSVDKVLTASSGTS+LYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQENKDSTNFPGRLAKLSVDKVLTASSGTSILYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 283

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 283

BLAST of Cp4.1LG18g07290 vs. ExPASy TrEMBL
Match: A0A6J1JLM1 (Cysteine dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485706 PE=3 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 7.15e-198
Identity = 272/303 (89.77%), Postives = 277/303 (91.42%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQ+ASDKIRLILQNKVDCSVFVRS SMNHR+SSKVQA+Y LCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQRASDKIRLILQNKVDCSVFVRSFSMNHRKSSKVQALYHLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSP DFGLKEENIGDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPEDFGLKEENIGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIF FPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFSFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQESKDSTNF GRLAKL+VD+VLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQESKDSTNFQGRLAKLAVDEVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVY GPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYTGPA 283

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 283

BLAST of Cp4.1LG18g07290 vs. ExPASy TrEMBL
Match: A0A6J1E097 (Cysteine dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111429658 PE=3 SV=1)

HSP 1 Score: 546 bits (1406), Expect = 1.46e-195
Identity = 272/303 (89.77%), Postives = 274/303 (90.43%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSPGDFGLKEEN+GDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPGDFGLKEENMGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE         RLAKLSVDKVLTASSGTS+LYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQER--------RLAKLSVDKVLTASSGTSILYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 275

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 275

BLAST of Cp4.1LG18g07290 vs. ExPASy TrEMBL
Match: A0A6J1JJN1 (Cysteine dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485706 PE=3 SV=1)

HSP 1 Score: 528 bits (1361), Expect = 1.05e-188
Identity = 264/303 (87.13%), Postives = 269/303 (88.78%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSKFQ+ASDKIRLILQNKVDCSVFVRS SMNHR+SSKVQA+Y LCKTSLTPTSSPLSSQ
Sbjct: 1   MFSKFQRASDKIRLILQNKVDCSVFVRSFSMNHRKSSKVQALYHLCKTSLTPTSSPLSSQ 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
           TIQNLCSLL                    DTVSP DFGLKEENIGDDRGHGPFGLSQFNR
Sbjct: 61  TIQNLCSLL--------------------DTVSPEDFGLKEENIGDDRGHGPFGLSQFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           IARWAQPITYIDIFEGESFTICIF FPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE
Sbjct: 121 IARWAQPITYIDIFEGESFTICIFSFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180

Query: 181 PAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240
           PAFIQE         RLAKL+VD+VLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP
Sbjct: 181 PAFIQER--------RLAKLAVDEVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSP 240

Query: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYAGPA 300
           PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVY GPA
Sbjct: 241 PYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAWLAKIETPDDLYMRPGVYTGPA 275

Query: 301 IQI 303
           IQI
Sbjct: 301 IQI 275

BLAST of Cp4.1LG18g07290 vs. ExPASy TrEMBL
Match: A0A1S4E0B0 (Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 6.60e-174
Identity = 245/334 (73.35%), Postives = 268/334 (80.24%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           MFSK +KA+DK+RL LQNKVDCSVF+R LSM+H++SSK+QAIYDLCK SLTP +SPLSSQ
Sbjct: 1   MFSKTRKATDKMRLFLQNKVDCSVFLRFLSMSHKKSSKIQAIYDLCKKSLTPANSPLSSQ 60

Query: 61  TIQNLCSLL---------------------------VLGLHEIDG----LNHWFLYAPFT 120
           TI+NLC+LL                           +  LH+       L    LYAPFT
Sbjct: 61  TIKNLCTLLEPLFFRECSLNILRDGLLFFSHGFGLILFPLHQFQSSFNCLEWSMLYAPFT 120

Query: 121 DTVSPGDFGLKEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTS 180
           DTV  GD GLKEEN+ DDRGHG FGLSQ NRIARWAQPITYIDI E E+FTICIFCFPTS
Sbjct: 121 DTVGAGDVGLKEENVDDDRGHGLFGLSQLNRIARWAQPITYIDICECENFTICIFCFPTS 180

Query: 181 SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTAS 240
           SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEP FIQESK S  F  RLAKL+VDKVLTAS
Sbjct: 181 SVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPPFIQESKGSPYFSARLAKLAVDKVLTAS 240

Query: 241 SGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNL 300
           S TSVLYPK GGN+HCFTAVSPCAVLDVLSPPYNEDKGRRCTY+HDYPYST S+++K  L
Sbjct: 241 SATSVLYPKAGGNIHCFTAVSPCAVLDVLSPPYNEDKGRRCTYYHDYPYSTISSDTKLYL 300

Query: 301 NDEEVEDYAWLAKIETPDDLYMRPGVYAGPAIQI 303
           NDEEVED+AWLAKIETPDDLYMRPG+Y GP IQI
Sbjct: 301 NDEEVEDHAWLAKIETPDDLYMRPGMYVGPTIQI 334

BLAST of Cp4.1LG18g07290 vs. TAIR 10
Match: AT1G18490.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 326.2 bits (835), Expect = 2.7e-89
Identity = 166/306 (54.25%), Postives = 206/306 (67.32%), Query Frame = 0

Query: 1   MFSKFQKASDKIRLILQNKVDCSVFVRSLSMNHRQSSKVQAIYDLCKTSLTPTSSPLSSQ 60
           M S+  KA +K+   L +K D    +   S N  +S KVQ +YDLCK + T  +   +S 
Sbjct: 1   MLSRLFKAGEKVLSNLVSKKD----IYMASRNQEKSPKVQELYDLCKETFTGKAPSPASM 60

Query: 61  TIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGLKEENIGDDRGHGPFGLSQFNR 120
            IQ LCS+L                    D+VSP D GL+E +  DDRG+G  G+S+FNR
Sbjct: 61  AIQKLCSVL--------------------DSVSPADVGLEEVSQDDDRGYGVSGVSRFNR 120

Query: 121 IARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVE 180
           + RWAQPIT++DI E ++FT+CIFCFPTSSVIPLHDHP M VFSK+LYGSLHVKAYDWVE
Sbjct: 121 VGRWAQPITFLDIHECDTFTMCIFCFPTSSVIPLHDHPEMAVFSKILYGSLHVKAYDWVE 180

Query: 181 PAFI--QESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVL 240
           P  I  Q+     + P RLAKL  DKV+T  S    LYPKTGGNLHCFTA++PCAVLD+L
Sbjct: 181 PPCIITQDKGVPGSLPARLAKLVSDKVITPQSEIPALYPKTGGNLHCFTALTPCAVLDIL 240

Query: 241 SPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVED-YAWLAKIETPDDLYMRPGVYA 300
           SPPY E  GR C+Y+ DYP+STF+ E+     DE  ED YAWL +I+TPDDL+MRPG Y 
Sbjct: 241 SPPYKESVGRSCSYYMDYPFSTFALENGMKKVDEGKEDEYAWLVQIDTPDDLHMRPGSYT 282

Query: 301 GPAIQI 304
           GP I++
Sbjct: 301 GPTIRV 282

BLAST of Cp4.1LG18g07290 vs. TAIR 10
Match: AT5G39890.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 195.3 bits (495), Expect = 7.2e-50
Identity = 99/215 (46.05%), Postives = 134/215 (62.33%), Query Frame = 0

Query: 90  DTVSPGDFGLKEENIGDDRGHGPFGLSQFNRIARWAQP-ITYIDIFEGESFTICIFCFPT 149
           D + P D G+  +            +S F        P +TY+ I+    F+ICIFC P 
Sbjct: 80  DEIKPEDVGVNPK------------MSYFRSTVTGRSPLVTYLHIYACHRFSICIFCLPP 139

Query: 150 SSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTA 209
           S VIPLH+HP MTVFSK+L+G++H+K+YDWV P   Q S D+     RLAK+ VD   TA
Sbjct: 140 SGVIPLHNHPEMTVFSKLLFGTMHIKSYDWV-PDSPQPSSDT-----RLAKVKVDSDFTA 199

Query: 210 SSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPN 269
              TS+LYP  GGN+HCFTA + CAVLDV+ PPY++  GR CTY+ DYP+S+FS +    
Sbjct: 200 PCDTSILYPADGGNMHCFTAKTACAVLDVIGPPYSDPAGRHCTYYFDYPFSSFSVDGVV- 259

Query: 270 LNDEEVEDYAWL-AKIETPDDLYMRPGVYAGPAIQ 303
           + +EE E YAWL  + E P+DL +   +Y+GP I+
Sbjct: 260 VAEEEKEGYAWLKEREEKPEDLTVTALMYSGPTIK 275

BLAST of Cp4.1LG18g07290 vs. TAIR 10
Match: AT5G15120.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 183.7 bits (465), Expect = 2.2e-46
Identity = 91/184 (49.46%), Postives = 124/184 (67.39%), Query Frame = 0

Query: 122 ARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPGMTVFSKVLYGSLHVKAYDWVEP 181
           AR + PITY+ + + + F+I IFC P S VIPLH+HPGMTVFSK+L+G++H+K+YDWV  
Sbjct: 115 ARSSPPITYLHLHQCDQFSIGIFCLPPSGVIPLHNHPGMTVFSKLLFGTMHIKSYDWVVD 174

Query: 182 AFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKTGGNLHCFTAVSPCAVLDVLSPP 241
           A +++SK       RLAKL VD   TA    S+LYP+ GGN+H FTA++ CAVLDVL PP
Sbjct: 175 APMRDSKT------RLAKLKVDSTFTAPCNASILYPEDGGNMHRFTAITACAVLDVLGPP 234

Query: 242 YNEDKGRRCTYFHDYPYSTFSAESKPNL-NDEEVEDYAWL-AKIETPDDLYMRPG-VYAG 301
           Y   +GR CTYF ++P    S+E    L ++EE E YAWL  + + P+D     G +Y G
Sbjct: 235 YCNPEGRHCTYFLEFPLDKLSSEDDDVLSSEEEKEGYAWLQERDDNPEDHTNVVGALYRG 292

Query: 302 PAIQ 303
           P ++
Sbjct: 295 PKVE 292

BLAST of Cp4.1LG18g07290 vs. TAIR 10
Match: AT2G42670.1 (Protein of unknown function (DUF1637) )

HSP 1 Score: 182.2 bits (461), Expect = 6.3e-46
Identity = 95/263 (36.12%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 40  QAIYDLCKTSLTPTSSPLSSQTIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGL 99
           Q +Y+ CK S + +  P++   ++ + ++L                    + + P D G+
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVL--------------------EKIKPSDVGI 65

Query: 100 KEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPG 159
           +++        GP  L++ N   +    I Y+ + E +SF+I IFC P SS+IPLH+HPG
Sbjct: 66  EQDAQLARSRSGP--LNERNGSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPG 125

Query: 160 MTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKT 219
           MTV SK++YGS+HVK+YDW+EP   +    S   P +L K   D  +TA S  + LYPK+
Sbjct: 126 MTVLSKLVYGSMHVKSYDWLEPQLTEPEDPSQARPAKLVK---DTEMTAQSPVTTLYPKS 185

Query: 220 GGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAW 279
           GGN+HCF A++ CA+LD+L+PPY+ +  R CTYF          E +  ++ E V D  W
Sbjct: 186 GGNIHCFKAITHCAILDILAPPYSSEHDRHCTYFRKSRREDLPGELE--VDGEVVTDVTW 240

Query: 280 LAKIETPDDLYMRPGVYAGPAIQ 303
           L + + PDD  +R   Y GP I+
Sbjct: 246 LEEFQPPDDFVIRRIPYRGPVIR 240

BLAST of Cp4.1LG18g07290 vs. TAIR 10
Match: AT2G42670.2 (Protein of unknown function (DUF1637) )

HSP 1 Score: 181.8 bits (460), Expect = 8.2e-46
Identity = 96/263 (36.50%), Postives = 146/263 (55.51%), Query Frame = 0

Query: 40  QAIYDLCKTSLTPTSSPLSSQTIQNLCSLLVLGLHEIDGLNHWFLYAPFTDTVSPGDFGL 99
           Q +Y+ CK S + +  P++   ++ + ++L                    + + P D G+
Sbjct: 6   QRLYNTCKASFS-SDGPITEDALEKVRNVL--------------------EKIKPSDVGI 65

Query: 100 KEENIGDDRGHGPFGLSQFNRIARWAQPITYIDIFEGESFTICIFCFPTSSVIPLHDHPG 159
           +++        GP  L++ N   +    I Y+ + E +SF+I IFC P SS+IPLH+HPG
Sbjct: 66  EQDAQLARSRSGP--LNERNGSNQSPPAIKYLHLHECDSFSIGIFCMPPSSMIPLHNHPG 125

Query: 160 MTVFSKVLYGSLHVKAYDWVEPAFIQESKDSTNFPGRLAKLSVDKVLTASSGTSVLYPKT 219
           MTV SK++YGS+HVK+YDW+EP   +    S     R AKL  D  +TA S  + LYPK+
Sbjct: 126 MTVLSKLVYGSMHVKSYDWLEPQLTEPEDPSQE--ARPAKLVKDTEMTAQSPVTTLYPKS 185

Query: 220 GGNLHCFTAVSPCAVLDVLSPPYNEDKGRRCTYFHDYPYSTFSAESKPNLNDEEVEDYAW 279
           GGN+HCF A++ CA+LD+L+PPY+ +  R CTYF          E +  ++ E V D  W
Sbjct: 186 GGNIHCFKAITHCAILDILAPPYSSEHDRHCTYFRKSRREDLPGELE--VDGEVVTDVTW 241

Query: 280 LAKIETPDDLYMRPGVYAGPAIQ 303
           L + + PDD  +R   Y GP I+
Sbjct: 246 LEEFQPPDDFVIRRIPYRGPVIR 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q1G3U63.8e-8854.25Plant cysteine oxidase 3 OS=Arabidopsis thaliana OX=3702 GN=PCO3 PE=1 SV=1[more]
Q8LGJ51.0e-4846.05Plant cysteine oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PCO2 PE=1 SV=1[more]
Q9LXG93.0e-4549.46Plant cysteine oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=PCO1 PE=1 SV=1[more]
Q9SJI98.9e-4536.12Plant cysteine oxidase 4 OS=Arabidopsis thaliana OX=3702 GN=PCO4 PE=1 SV=2[more]
Q9LXT42.2e-4336.36Plant cysteine oxidase 5 OS=Arabidopsis thaliana OX=3702 GN=PCO5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023516979.13.02e-20693.40plant cysteine oxidase 3 [Cucurbita pepo subsp. pepo][more]
XP_022921368.13.53e-20592.41plant cysteine oxidase 3 isoform X1 [Cucurbita moschata][more]
XP_022988474.11.48e-19789.77plant cysteine oxidase 3 isoform X1 [Cucurbita maxima][more]
XP_022921369.13.02e-19589.77plant cysteine oxidase 3 isoform X2 [Cucurbita moschata][more]
XP_022988475.12.18e-18887.13plant cysteine oxidase 3 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1E3Q91.71e-20592.41Cysteine dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111429658 PE=3 SV=1[more]
A0A6J1JLM17.15e-19889.77Cysteine dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485706 PE=3 SV=1[more]
A0A6J1E0971.46e-19589.77Cysteine dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111429658 PE=3 SV=1[more]
A0A6J1JJN11.05e-18887.13Cysteine dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111485706 PE=3 SV=1[more]
A0A1S4E0B06.60e-17473.35Cysteine dioxygenase OS=Cucumis melo OX=3656 GN=LOC103494087 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G18490.12.7e-8954.25Protein of unknown function (DUF1637) [more]
AT5G39890.17.2e-5046.05Protein of unknown function (DUF1637) [more]
AT5G15120.12.2e-4649.46Protein of unknown function (DUF1637) [more]
AT2G42670.16.3e-4636.12Protein of unknown function (DUF1637) [more]
AT2G42670.28.2e-4636.50Protein of unknown function (DUF1637) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012864Cysteine oxygenase/2-aminoethanethiol dioxygenasePFAMPF07847PCO_ADOcoord: 90..301
e-value: 2.1E-69
score: 233.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 116..268
e-value: 4.9E-13
score: 50.7
NoneNo IPR availablePANTHERPTHR22966:SF292-AMINOETHANETHIOL DIOXYGENASEcoord: 31..302
NoneNo IPR availablePANTHERPTHR22966UNCHARACTERIZEDcoord: 31..302
NoneNo IPR availableCDDcd20289cupin_ADOcoord: 137..243
e-value: 3.72481E-50
score: 159.638
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 125..264

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g07290.1Cp4.1LG18g07290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0017172 cysteine dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen