Cp4.1LG18g05610 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG18g05610 vs. ExPASy Swiss-Prot
Match: P48627 (Omega-6 fatty acid desaturase, chloroplastic OS=Brassica napus OX=3708 PE=1 SV=1)

HSP 1 Score: 686.0 bits (1769), Expect = 2.9e-196
Identity = 323/442 (73.08%), Postives = 374/442 (84.62%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIAS-HYSPGM--KREVMFQKKIKRQNYVLPV-KRAAIIRAVAVPVAP 92
           F GP+Q   R  K+AS   SPG+   R +    K  R+ +++P  KR   I+AV VPVA 
Sbjct: 12  FTGPQQCLPRAPKLASARLSPGVYAVRPIDLLLKGTRRTFLVPAKKRIGCIKAVFVPVA- 71

Query: 93  LPSSADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSV 152
            P SAD+AE R++L+ESYGF++IG+ LP++V LKDI+++LPKEVFEI+DVKAWKSVL+SV
Sbjct: 72  -PPSADNAEDREQLAESYGFKQIGQDLPDNVTLKDIMDTLPKEVFEIDDVKAWKSVLISV 131

Query: 153 ASCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVE 212
            S ALGL MIAKA WYLLPLAWAWTGTA+TG         FFVIGHDCAHKSFS+NKLVE
Sbjct: 132 TSYALGLFMIAKAPWYLLPLAWAWTGTAVTG---------FFVIGHDCAHKSFSKNKLVE 191

Query: 213 DIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGY 272
           DIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEFDS P+LRKAI++GY
Sbjct: 192 DIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFDSSPVLRKAIIFGY 251

Query: 273 GPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKF 332
           GP RPW+SIAHW+ WHF+++KFRP+EV RVKISLACVFAFM +GWPLIIYK G++GWVKF
Sbjct: 252 GPIRPWLSIAHWVNWHFNLRKFRPSEVNRVKISLACVFAFMAVGWPLIIYKVGVLGWVKF 311

Query: 333 WLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHIN 392
           WLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEILCH IN
Sbjct: 312 WLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEILCHDIN 371

Query: 393 VHIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAF 452
           VHIPHHISPRIPSYNL AAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KEENY+ F
Sbjct: 372 VHIPHHISPRIPSYNLRAAHQSIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEENYIPF 431

Query: 453 DRLNPEDSYPITFLRKVMPDFS 471
           DRL PE+S PITFL+K MPD++
Sbjct: 432 DRLAPEESQPITFLKKAMPDYA 442

BLAST of Cp4.1LG18g05610 vs. ExPASy Swiss-Prot
Match: P48628 (Omega-6 fatty acid desaturase, chloroplastic OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 5.7e-192
Identity = 315/424 (74.29%), Postives = 350/424 (82.55%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIASHYSPG---MKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPL 92
           F G  Q       IA+ YSPG   +    + QK+ +RQ   +   +  +I AVA+PV P 
Sbjct: 12  FKGSYQKPVLRRDIAARYSPGIFSLNSNGLIQKRFRRQRNFVTRNKVTVIHAVAIPVQPA 71

Query: 93  PSSADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVA 152
           P   +SAEYRK+L+E YGF ++GEPL +DV LKD+I  LPKEVFEI+DVKAWKSVL+SV 
Sbjct: 72  P--VESAEYRKQLAEDYGFRQVGEPLSDDVTLKDVINPLPKEVFEIDDVKAWKSVLISVT 131

Query: 153 SCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVED 212
           S ALGL MI+KA WYLLPLAW WTGTAITG         FFVIGHDCAH+SFS NKLVED
Sbjct: 132 SYALGLFMISKAPWYLLPLAWVWTGTAITG---------FFVIGHDCAHRSFSSNKLVED 191

Query: 213 IVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYG 272
           IVGTLAFMPLIYPYEPWRFKHD+HHAKTNML+EDTAWHPVWK+EF+S PLLRKAI+YGYG
Sbjct: 192 IVGTLAFMPLIYPYEPWRFKHDRHHAKTNMLREDTAWHPVWKDEFESTPLLRKAIIYGYG 251

Query: 273 PFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFW 332
           PFR WMSIAHWLMWHFD+KKFRP+EV RVKISLACVFAF+ IGWPLIIYK GI+GW+KFW
Sbjct: 252 PFRCWMSIAHWLMWHFDLKKFRPSEVPRVKISLACVFAFIAIGWPLIIYKTGIMGWIKFW 311

Query: 333 LMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINV 392
           LMPWLGYHFWMSTFTMVHHTAP+IPFK SEEWN AQAQLNGTVHC YPKWIEILCH INV
Sbjct: 312 LMPWLGYHFWMSTFTMVHHTAPYIPFKYSEEWNRAQAQLNGTVHCDYPKWIEILCHDINV 371

Query: 393 HIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFD 452
           HIPHHISPRIPSYNL AAHKSLQENWG+YLNEASWNWRLMKTIMT+C VY KE++     
Sbjct: 372 HIPHHISPRIPSYNLRAAHKSLQENWGQYLNEASWNWRLMKTIMTVCQVYDKEKSLCCLR 424

Query: 453 RLNP 454
           R  P
Sbjct: 432 RTCP 424

BLAST of Cp4.1LG18g05610 vs. ExPASy Swiss-Prot
Match: P46312 (Omega-6 fatty acid desaturase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAD6 PE=1 SV=2)

HSP 1 Score: 669.5 bits (1726), Expect = 2.8e-191
Identity = 319/447 (71.36%), Postives = 368/447 (82.33%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIA---SHYSPGMKREVMFQKKIKRQNY-----VLPVK-RAAIIRAVA 92
           F GP+Q   R  K+A   +  SPG+         +K + +     V PVK R   I+AVA
Sbjct: 12  FTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPVKRRIGCIKAVA 71

Query: 93  VPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKS 152
            PVA  P SADSAE R++L+ESYGF +IGE LPE+V LKDI+++LPKEVFEI+D+KA KS
Sbjct: 72  APVA--PPSADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKALKS 131

Query: 153 VLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSR 212
           VL+SV S  LGL MIAK+ WYLLPLAWAWTGTAITG         FFVIGHDCAHKSFS+
Sbjct: 132 VLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSK 191

Query: 213 NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKA 272
           NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S P++RKA
Sbjct: 192 NKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRKA 251

Query: 273 IVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGII 332
           I++GYGP RPW+SIAHW+ WHF++KKFR +EV RVKISLACVFAFM +GWPLI+YK GI+
Sbjct: 252 IIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGIL 311

Query: 333 GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEIL 392
           GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEIL
Sbjct: 312 GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEIL 371

Query: 393 CHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEE 452
           CH INVHIPHHISPRIPSYNL AAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KEE
Sbjct: 372 CHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEE 431

Query: 453 NYVAFDRLNPEDSYPITFLRKVMPDFS 471
           NY+ FDRL PE+S PITFL+K MP+++
Sbjct: 432 NYIPFDRLAPEESQPITFLKKAMPNYT 447

BLAST of Cp4.1LG18g05610 vs. ExPASy Swiss-Prot
Match: P48629 (Omega-6 fatty acid desaturase, chloroplastic OS=Spinacia oleracea OX=3562 GN=FAD6 PE=1 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 1.8e-177
Identity = 286/400 (71.50%), Postives = 340/400 (85.00%), Query Frame = 0

Query: 74  KRAAIIRAVAVPVAPLPSS---ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPK 133
           +++ +++AVAVPVA  PS+    D+ E+ K+L+E YGF++IGEPLP+DV ++DII SLPK
Sbjct: 58  RKSTLVQAVAVPVAQ-PSAFPPTDNTEHLKQLAERYGFQQIGEPLPDDVTMRDIITSLPK 117

Query: 134 EVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFF 193
           +VFEIND KAW +VL+SV S ALG+ MIAKA WYLLPLAWAWTGTAITG         FF
Sbjct: 118 QVFEINDTKAWGTVLISVTSYALGIFMIAKAPWYLLPLAWAWTGTAITG---------FF 177

Query: 194 VIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVW 253
           VIGHDCAHKSFS+NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHH KTNML+EDTAW P+ 
Sbjct: 178 VIGHDCAHKSFSKNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHTKTNMLREDTAWLPIM 237

Query: 254 KEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMF 313
           KE+ +S P LRKA++Y YGP R WMSIAHWL  HF++K FR +EVKR  ISLA VFAFM 
Sbjct: 238 KEDIESSPGLRKALIYAYGPLRTWMSIAHWLKVHFNLKDFRQSEVKRATISLAAVFAFMV 297

Query: 314 IGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNG 373
           IGWPLIIYK GI+GW+KFWLMPWLGYHFWMSTFT+VHHTAPHIPFKSS+EWNAAQAQL+G
Sbjct: 298 IGWPLIIYKTGIVGWIKFWLMPWLGYHFWMSTFTIVHHTAPHIPFKSSKEWNAAQAQLSG 357

Query: 374 TVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMK 433
           TVHC YP+WIEILCH I+VHIPHHISP+IPSYNL AA++SL ENWG+YLN+   NWRLM+
Sbjct: 358 TVHCDYPRWIEILCHDISVHIPHHISPKIPSYNLRAANQSLNENWGEYLNKPKSNWRLMR 417

Query: 434 TIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 471
           TIMT CH+Y K+ NYV+F++  PE+S PI+  ++VMPD++
Sbjct: 418 TIMTTCHIYDKDGNYVSFEKAVPEESQPISIPKRVMPDYA 447

BLAST of Cp4.1LG18g05610 vs. ExPASy Swiss-Prot
Match: P20388 (Delta(12)-fatty-acid desaturase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=desA PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.5e-102
Identity = 171/335 (51.04%), Postives = 224/335 (66.87%), Query Frame = 0

Query: 118 DVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAI 177
           D++L+DII++LPKE FE    KAW SVL+++ + A+G + I    WY LP+ W WTGTA+
Sbjct: 23  DLKLQDIIKTLPKECFEKKASKAWASVLITLGAIAVGYLGIIYLPWYCLPITWIWTGTAL 82

Query: 178 TGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKT 237
           TGA         FV+GHDC H+SF++ + V D+VG +AF PLIYP+  WR  HD HH  T
Sbjct: 83  TGA---------FVVGHDCGHRSFAKKRWVNDLVGHIAFAPLIYPFHSWRLLHDHHHLHT 142

Query: 238 NMLKEDTAWHPVWKEEFDSDPLLRKAIVYGY-GPFRPWMSIAHWLMWHFDVKKFRPNEVK 297
           N ++ D AW P   E F + P + +       GPF    SI HW + HF +  F   +  
Sbjct: 143 NKIEVDNAWDPWSVEAFQASPAIVRLFYRAIRGPFWWTGSIFHWSLMHFKLSNFAQRDRN 202

Query: 298 RVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK 357
           +VK+S+A VF F  I +P +I   G+ G+VKFWLMPWL YHFWMSTFT+VHHT P I F+
Sbjct: 203 KVKLSIAVVFLFAAIAFPALIITTGVWGFVKFWLMPWLVYHFWMSTFTIVHHTIPEIRFR 262

Query: 358 SSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWG 417
            + +W+AA+AQLNGTVHC YP+W+E+LCH INVHIPHH+S  IPSYNL  AH SL+ENWG
Sbjct: 263 PAADWSAAEAQLNGTVHCDYPRWVEVLCHDINVHIPHHLSVAIPSYNLRLAHGSLKENWG 322

Query: 418 KYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRL 452
            +L E ++NW+LM+ I   CH+Y  E  Y  F  L
Sbjct: 323 PFLYERTFNWQLMQQISGQCHLYDPEHGYRTFGSL 348

BLAST of Cp4.1LG18g05610 vs. NCBI nr
Match: KAG6589776.1 (hypothetical protein SDJN03_15199, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 462/470 (98.30%), Postives = 463/470 (98.51%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           ME NSRLLAGRKSSNISAAFHTSGGFSKR TPFMGPRQNSART KIASHYSPGMKREVMF
Sbjct: 1   MEENSRLLAGRKSSNISAAFHTSGGFSKRITPFMGPRQNSARTRKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAI RAVAVPVAPLPSSADSAEYRK LSESYGFEKIGEPLPEDVR
Sbjct: 61  QKKIKRQNYVLPVKRAAITRAVAVPVAPLPSSADSAEYRKGLSESYGFEKIGEPLPEDVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKA WYLLPLAWAWTGTAITGA
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAPWYLLPLAWAWTGTAITGA 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
           TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYG FRPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGLFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDF+
Sbjct: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFA 470

BLAST of Cp4.1LG18g05610 vs. NCBI nr
Match: XP_023515614.1 (omega-6 fatty acid desaturase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 461/470 (98.09%), Postives = 461/470 (98.09%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF
Sbjct: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR
Sbjct: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITG 
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITG- 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
                   FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 --------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS
Sbjct: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 461

BLAST of Cp4.1LG18g05610 vs. NCBI nr
Match: XP_022921546.1 (omega-6 fatty acid desaturase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 453/470 (96.38%), Postives = 454/470 (96.60%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           ME NSRLLAGRKSSNISAAFHTSGGFSKR TPFMGPRQNSART KIASHYSPGMKREVMF
Sbjct: 1   MEENSRLLAGRKSSNISAAFHTSGGFSKRITPFMGPRQNSARTRKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAI RAVAVPVAPLPSSADSAEYRK LSESYGFEKIGEPLPEDVR
Sbjct: 61  QKKIKRQNYVLPVKRAAITRAVAVPVAPLPSSADSAEYRKGLSESYGFEKIGEPLPEDVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKSVLVSVASCAL LIMIAKA WYLLPLAWAWTGTAITG 
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALSLIMIAKAPWYLLPLAWAWTGTAITG- 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
                   FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 --------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDF+
Sbjct: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFA 461

BLAST of Cp4.1LG18g05610 vs. NCBI nr
Match: XP_022988573.1 (omega-6 fatty acid desaturase, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 447/470 (95.11%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           MEANSRLLAGRKSSNISAAFHTSGGFSKR TPFMGPRQNSART KIASHYSPGMKREVMF
Sbjct: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRVTPFMGPRQNSARTQKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAIIRAVA+PVAPLPSSADSAE+RK+LSESYGFEKIGEPLPE+VR
Sbjct: 61  QKKIKRQNYVLPVKRAAIIRAVAIPVAPLPSSADSAEHRKRLSESYGFEKIGEPLPENVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKS+LVSVAS ALGLIMIAKA WYLLPLAWAWTGTAITG 
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSILVSVASYALGLIMIAKAPWYLLPLAWAWTGTAITG- 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
                   FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 --------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYGP RPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPLRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAG+IGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGVIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNW+LMKTIMTICHVYSKEENYVAFDRLNP+DSYPITFLRKVMPDF+
Sbjct: 421 EASWNWQLMKTIMTICHVYSKEENYVAFDRLNPDDSYPITFLRKVMPDFA 461

BLAST of Cp4.1LG18g05610 vs. NCBI nr
Match: XP_023515615.1 (omega-6 fatty acid desaturase, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 428/438 (97.72%), Postives = 428/438 (97.72%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSS 92
           F GPRQNSARTHKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSS
Sbjct: 12  FTGPRQNSARTHKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSS 71

Query: 93  ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA 152
           ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA
Sbjct: 72  ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA 131

Query: 153 LGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVG 212
           LGLIMIAKAHWYLLPLAWAWTGTAITG         FFVIGHDCAHKSFSRNKLVEDIVG
Sbjct: 132 LGLIMIAKAHWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSRNKLVEDIVG 191

Query: 213 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR 272
           TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR
Sbjct: 192 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR 251

Query: 273 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP 332
           PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP
Sbjct: 252 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP 311

Query: 333 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 392
           WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP
Sbjct: 312 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 371

Query: 393 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN 452
           HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN
Sbjct: 372 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN 431

Query: 453 PEDSYPITFLRKVMPDFS 470
           PEDSYPITFLRKVMPDFS
Sbjct: 432 PEDSYPITFLRKVMPDFS 440

BLAST of Cp4.1LG18g05610 vs. ExPASy TrEMBL
Match: A0A6J1E1P1 (omega-6 fatty acid desaturase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429776 PE=4 SV=1)

HSP 1 Score: 938 bits (2425), Expect = 0.0
Identity = 453/470 (96.38%), Postives = 454/470 (96.60%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           ME NSRLLAGRKSSNISAAFHTSGGFSKR TPFMGPRQNSART KIASHYSPGMKREVMF
Sbjct: 1   MEENSRLLAGRKSSNISAAFHTSGGFSKRITPFMGPRQNSARTRKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAI RAVAVPVAPLPSSADSAEYRK LSESYGFEKIGEPLPEDVR
Sbjct: 61  QKKIKRQNYVLPVKRAAITRAVAVPVAPLPSSADSAEYRKGLSESYGFEKIGEPLPEDVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKSVLVSVASCAL LIMIAKA WYLLPLAWAWTGTAITG 
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALSLIMIAKAPWYLLPLAWAWTGTAITG- 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
                   FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 --------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDF+
Sbjct: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFA 461

BLAST of Cp4.1LG18g05610 vs. ExPASy TrEMBL
Match: A0A6J1JMP3 (omega-6 fatty acid desaturase, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485774 PE=4 SV=1)

HSP 1 Score: 932 bits (2408), Expect = 0.0
Identity = 447/470 (95.11%), Postives = 456/470 (97.02%), Query Frame = 0

Query: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRSTPFMGPRQNSARTHKIASHYSPGMKREVMF 60
           MEANSRLLAGRKSSNISAAFHTSGGFSKR TPFMGPRQNSART KIASHYSPGMKREVMF
Sbjct: 1   MEANSRLLAGRKSSNISAAFHTSGGFSKRVTPFMGPRQNSARTQKIASHYSPGMKREVMF 60

Query: 61  QKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVR 120
           QKKIKRQNYVLPVKRAAIIRAVA+PVAPLPSSADSAE+RK+LSESYGFEKIGEPLPE+VR
Sbjct: 61  QKKIKRQNYVLPVKRAAIIRAVAIPVAPLPSSADSAEHRKRLSESYGFEKIGEPLPENVR 120

Query: 121 LKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGA 180
           LKDIIESLPKEVFEINDVKAWKS+LVSVAS ALGLIMIAKA WYLLPLAWAWTGTAITG 
Sbjct: 121 LKDIIESLPKEVFEINDVKAWKSILVSVASYALGLIMIAKAPWYLLPLAWAWTGTAITG- 180

Query: 181 TEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240
                   FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML
Sbjct: 181 --------FFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNML 240

Query: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300
           KEDTAWHPVWKEEFDSDPLLRKAIVYGYGP RPWMSIAHWLMWHFDVKKFRPNEVKRVKI
Sbjct: 241 KEDTAWHPVWKEEFDSDPLLRKAIVYGYGPLRPWMSIAHWLMWHFDVKKFRPNEVKRVKI 300

Query: 301 SLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360
           SLACVFAFMFIGWPLIIYKAG+IGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE
Sbjct: 301 SLACVFAFMFIGWPLIIYKAGVIGWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEE 360

Query: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420
           WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN
Sbjct: 361 WNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLN 420

Query: 421 EASWNWRLMKTIMTICHVYSKEENYVAFDRLNPEDSYPITFLRKVMPDFS 470
           EASWNW+LMKTIMTICHVYSKEENYVAFDRLNP+DSYPITFLRKVMPDF+
Sbjct: 421 EASWNWQLMKTIMTICHVYSKEENYVAFDRLNPDDSYPITFLRKVMPDFA 461

BLAST of Cp4.1LG18g05610 vs. ExPASy TrEMBL
Match: A0A6J1E0T0 (omega-6 fatty acid desaturase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111429776 PE=4 SV=1)

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 422/438 (96.35%), Postives = 423/438 (96.58%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSS 92
           F GPRQNSART KIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAI RAVAVPVAPLPSS
Sbjct: 12  FTGPRQNSARTRKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAITRAVAVPVAPLPSS 71

Query: 93  ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA 152
           ADSAEYRK LSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA
Sbjct: 72  ADSAEYRKGLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA 131

Query: 153 LGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVG 212
           L LIMIAKA WYLLPLAWAWTGTAITG         FFVIGHDCAHKSFSRNKLVEDIVG
Sbjct: 132 LSLIMIAKAPWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSRNKLVEDIVG 191

Query: 213 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR 272
           TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR
Sbjct: 192 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR 251

Query: 273 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP 332
           PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP
Sbjct: 252 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP 311

Query: 333 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 392
           WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP
Sbjct: 312 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 371

Query: 393 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN 452
           HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN
Sbjct: 372 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN 431

Query: 453 PEDSYPITFLRKVMPDFS 470
           PEDSYPITFLRKVMPDF+
Sbjct: 432 PEDSYPITFLRKVMPDFA 440

BLAST of Cp4.1LG18g05610 vs. ExPASy TrEMBL
Match: A0A6J1JJY6 (omega-6 fatty acid desaturase, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485774 PE=4 SV=1)

HSP 1 Score: 874 bits (2259), Expect = 0.0
Identity = 415/438 (94.75%), Postives = 424/438 (96.80%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPLPSS 92
           F GPRQNSART KIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVA+PVAPLPSS
Sbjct: 12  FTGPRQNSARTQKIASHYSPGMKREVMFQKKIKRQNYVLPVKRAAIIRAVAIPVAPLPSS 71

Query: 93  ADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCA 152
           ADSAE+RK+LSESYGFEKIGEPLPE+VRLKDIIESLPKEVFEINDVKAWKS+LVSVAS A
Sbjct: 72  ADSAEHRKRLSESYGFEKIGEPLPENVRLKDIIESLPKEVFEINDVKAWKSILVSVASYA 131

Query: 153 LGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVG 212
           LGLIMIAKA WYLLPLAWAWTGTAITG         FFVIGHDCAHKSFSRNKLVEDIVG
Sbjct: 132 LGLIMIAKAPWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSRNKLVEDIVG 191

Query: 213 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFR 272
           TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGP R
Sbjct: 192 TLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPLR 251

Query: 273 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMP 332
           PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAG+IGWVKFWLMP
Sbjct: 252 PWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGVIGWVKFWLMP 311

Query: 333 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 392
           WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP
Sbjct: 312 WLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIP 371

Query: 393 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFDRLN 452
           HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNW+LMKTIMTICHVYSKEENYVAFDRLN
Sbjct: 372 HHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWQLMKTIMTICHVYSKEENYVAFDRLN 431

Query: 453 PEDSYPITFLRKVMPDFS 470
           P+DSYPITFLRKVMPDF+
Sbjct: 432 PDDSYPITFLRKVMPDFA 440

BLAST of Cp4.1LG18g05610 vs. ExPASy TrEMBL
Match: A0A6J1C240 (omega-6 fatty acid desaturase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111007246 PE=4 SV=1)

HSP 1 Score: 826 bits (2134), Expect = 6.95e-301
Identity = 393/441 (89.12%), Postives = 415/441 (94.10%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIASHYSPGM---KREVMFQKKIKRQNYVLPVKRAAIIRAVAVPVAPL 92
           F GP QNSART K ASHYSPG+   K E++FQKKIKR+NY+LPVKRAAI+RAVAVPVAP 
Sbjct: 12  FTGPHQNSARTQKTASHYSPGILHVKGEIIFQKKIKRRNYLLPVKRAAILRAVAVPVAP- 71

Query: 93  PSSADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVA 152
           P+SADS+EYRK+LSESYGFEKIGEPLPE+VRLKDI+ESLPKEVFEINDVKAWKSVLVSVA
Sbjct: 72  PTSADSSEYRKQLSESYGFEKIGEPLPENVRLKDIVESLPKEVFEINDVKAWKSVLVSVA 131

Query: 153 SCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVED 212
           S  LGLIMIAKA WYLLPLAWAWTGTAITG         FFVIGHDCAHKSFS+NKLVED
Sbjct: 132 SYTLGLIMIAKAPWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSKNKLVED 191

Query: 213 IVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKAIVYGYG 272
           IVGTLAF+PLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDS PL+RKAI+YGYG
Sbjct: 192 IVGTLAFLPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSVPLMRKAIIYGYG 251

Query: 273 PFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFW 332
           PFRPWMSIAHWL+WHF++K FRPNEVKRVKISLACVFAFMFIGWPLIIYKAGI+GWVKFW
Sbjct: 252 PFRPWMSIAHWLLWHFNLKIFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGILGWVKFW 311

Query: 333 LMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINV 392
           LMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYP+WIEILCH INV
Sbjct: 312 LMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPQWIEILCHDINV 371

Query: 393 HIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVAFD 452
           HIPHHISPRIPSYNL AAHKSLQENWGKYLNEASWNWRLMKTIMT+CHVYSKEENYVAFD
Sbjct: 372 HIPHHISPRIPSYNLRAAHKSLQENWGKYLNEASWNWRLMKTIMTMCHVYSKEENYVAFD 431

Query: 453 RLNPEDSYPITFLRKVMPDFS 470
           RLNPEDSYPITFLRKVMPD +
Sbjct: 432 RLNPEDSYPITFLRKVMPDIA 442

BLAST of Cp4.1LG18g05610 vs. TAIR 10
Match: AT4G30950.1 (fatty acid desaturase 6 )

HSP 1 Score: 669.5 bits (1726), Expect = 2.0e-192
Identity = 319/447 (71.36%), Postives = 368/447 (82.33%), Query Frame = 0

Query: 33  FMGPRQNSARTHKIA---SHYSPGMKREVMFQKKIKRQNY-----VLPVK-RAAIIRAVA 92
           F GP+Q   R  K+A   +  SPG+         +K + +     V PVK R   I+AVA
Sbjct: 12  FTGPQQCLPRVPKLAASSARVSPGVYAVKPIDLLLKGRTHRSRRCVAPVKRRIGCIKAVA 71

Query: 93  VPVAPLPSSADSAEYRKKLSESYGFEKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKS 152
            PVA  P SADSAE R++L+ESYGF +IGE LPE+V LKDI+++LPKEVFEI+D+KA KS
Sbjct: 72  APVA--PPSADSAEDREQLAESYGFRQIGEDLPENVTLKDIMDTLPKEVFEIDDLKALKS 131

Query: 153 VLVSVASCALGLIMIAKAHWYLLPLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSR 212
           VL+SV S  LGL MIAK+ WYLLPLAWAWTGTAITG         FFVIGHDCAHKSFS+
Sbjct: 132 VLISVTSYTLGLFMIAKSPWYLLPLAWAWTGTAITG---------FFVIGHDCAHKSFSK 191

Query: 213 NKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTNMLKEDTAWHPVWKEEFDSDPLLRKA 272
           NKLVEDIVGTLAF+PL+YPYEPWRFKHD+HHAKTNML  DTAW PV  EEF+S P++RKA
Sbjct: 192 NKLVEDIVGTLAFLPLVYPYEPWRFKHDRHHAKTNMLVHDTAWQPVPPEEFESSPVMRKA 251

Query: 273 IVYGYGPFRPWMSIAHWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGII 332
           I++GYGP RPW+SIAHW+ WHF++KKFR +EV RVKISLACVFAFM +GWPLI+YK GI+
Sbjct: 252 IIFGYGPIRPWLSIAHWVNWHFNLKKFRASEVNRVKISLACVFAFMAVGWPLIVYKVGIL 311

Query: 333 GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEIL 392
           GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFK ++EWNAAQAQLNGTVHC YP WIEIL
Sbjct: 312 GWVKFWLMPWLGYHFWMSTFTMVHHTAPHIPFKPADEWNAAQAQLNGTVHCDYPSWIEIL 371

Query: 393 CHHINVHIPHHISPRIPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEE 452
           CH INVHIPHHISPRIPSYNL AAH+S+QENWGKY N A+WNWRLMKTIMT+CHVY KEE
Sbjct: 372 CHDINVHIPHHISPRIPSYNLRAAHESIQENWGKYTNLATWNWRLMKTIMTVCHVYDKEE 431

Query: 453 NYVAFDRLNPEDSYPITFLRKVMPDFS 471
           NY+ FDRL PE+S PITFL+K MP+++
Sbjct: 432 NYIPFDRLAPEESQPITFLKKAMPNYT 447

BLAST of Cp4.1LG18g05610 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 120.2 bits (300), Expect = 4.6e-27
Identity = 96/349 (27.51%), Postives = 153/349 (43.84%), Query Frame = 0

Query: 116 PEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAKA----HWYLLPLAWA 175
           P    L DI  ++PK  +  N    WKS+   V   A+   + A A    +W + PL W 
Sbjct: 93  PPPFNLADIRAAIPKHCWVKN---PWKSLSYVVRDVAIVFALAAGAAYLNNWIVWPLYWL 152

Query: 176 WTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHD 235
             GT           +  FV+GHDC H SFS +  +  +VG L    ++ PY  WR  H 
Sbjct: 153 AQGTMF---------WALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHR 212

Query: 236 QHHAKTNMLKEDTAWHPVWKEEFD-----------SDPLLRKAIVYGYGPFRPWMSIAHW 295
            HH     ++ D +WHP+ ++ ++           + PL+  A  +      P    +H 
Sbjct: 213 THHQNHGHVENDESWHPMSEKIYNTLDKPTRFFRFTLPLVMLAYPFYLWARSPGKKGSH- 272

Query: 296 LMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWM 355
             +H D   F P E K V  S AC  A   +    + +  G I  +K + +P+     W+
Sbjct: 273 --YHPDSDLFLPKERKDVLTSTACWTAMAAL-LVCLNFTIGPIQMLKLYGIPYWINVMWL 332

Query: 356 STFTMVHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPR 415
              T +HH      +P+   +EW+  +  L  T+   Y   I  + H I  H+ HH+ P+
Sbjct: 333 DFVTYLHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GLINNIHHDIGTHVIHHLFPQ 392

Query: 416 IPSYNLHAAHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVA 448
           IP Y+L  A ++ +   GKY  E   +  L   ++ I     KE++YV+
Sbjct: 393 IPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVS 423

BLAST of Cp4.1LG18g05610 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 117.1 bits (292), Expect = 3.9e-26
Identity = 85/341 (24.93%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 120 RLKDIIESLPKEVFEINDVKAWKSVLVS-VASCALGLIMIAKAHWYLLPLAWAWTGTAIT 179
           ++ DI  ++PK  +  + +++   V+   +A  AL +  +    W+L PL WA  GT   
Sbjct: 35  KIGDIRAAIPKHCWVKSPLRSMSYVVRDIIAVAALAIAAVYVDSWFLWPLYWAAQGTLF- 94

Query: 180 GATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPWRFKHDQHHAKTN 239
                   +  FV+GHDC H SFS   L+  +VG +    ++ PY  WR  H  HH    
Sbjct: 95  --------WAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQNHG 154

Query: 240 MLKEDTAWHPVWKEEFDSDPLLRKAIVYGYGPFRPWMSIAHWLMW--------HFD--VK 299
            ++ D +W P+ +  +   P   + + Y      P ++   +L +        HF+    
Sbjct: 155 HVENDESWVPLPERVYKKLPHSTRMLRYTVP--LPMLAYPLYLCYRSPGKEGSHFNPYSS 214

Query: 300 KFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHFWMSTFTMVHH 359
            F P+E K +  S  C ++ MF+    + +  G +  +K + +P++ +  W+   T +HH
Sbjct: 215 LFAPSERKLIATSTTC-WSIMFVSLIALSFVFGPLAVLKVYGVPYIIFVMWLDAVTYLHH 274

Query: 360 TA--PHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHISPRIPSYNLHA 419
                 +P+   +EW+  +  L  T+   Y  +  I  H I  H+ HH+ P+IP Y+L  
Sbjct: 275 HGHDEKLPWYRGKEWSYLRGGLT-TIDRDYGIFNNI-HHDIGTHVIHHLFPQIPHYHLVD 334

Query: 420 AHKSLQENWGKYLNEASWNWRLMKTIMTICHVYSKEENYVA 448
           A K+ +   G+Y  E   +  +   ++       K+++YV+
Sbjct: 335 ATKAAKHVLGRYYREPKTSGAIPIHLVESLVASIKKDHYVS 361

BLAST of Cp4.1LG18g05610 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 115.9 bits (289), Expect = 8.6e-26
Identity = 91/325 (28.00%), Postives = 140/325 (43.08%), Query Frame = 0

Query: 109 EKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAK--AHWYLL 168
           E+     P    L DI  ++PK  +  N   +   V+  VA    GL  +A    +W L 
Sbjct: 79  ERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLW 138

Query: 169 PLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPW 228
           PL W   GT           +  FV+GHDC H SFS +  +  + G L    ++ PY  W
Sbjct: 139 PLYWFAQGTMF---------WALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGW 198

Query: 229 RFKHDQHHAKTNMLKEDTAWHP----VWKEEFDSDPLLRKAIVYGY--GPFRPWMSI--A 288
           R  H  HH     ++ D +WHP    ++K    +  + R  + +     PF  W      
Sbjct: 199 RISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGK 258

Query: 289 HWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHF 348
               +H D   F P E K V  S AC  A   +    + +  G I  +K + +P+  +  
Sbjct: 259 QGSHYHPDSDLFLPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVM 318

Query: 349 WMSTFTMVHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHIS 408
           W+   T +HH      +P+   +EW+  +  L  T+   Y  WI  + H I  H+ HH+ 
Sbjct: 319 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLF 378

Query: 409 PRIPSYNLHAAHKSLQENWGKYLNE 422
           P+IP Y+L  A ++ +   GKY  E
Sbjct: 379 PQIPHYHLVEATEAAKPVLGKYYRE 390

BLAST of Cp4.1LG18g05610 vs. TAIR 10
Match: AT5G05580.2 (fatty acid desaturase 8 )

HSP 1 Score: 109.8 bits (273), Expect = 6.2e-24
Identity = 86/308 (27.92%), Postives = 132/308 (42.86%), Query Frame = 0

Query: 109 EKIGEPLPEDVRLKDIIESLPKEVFEINDVKAWKSVLVSVASCALGLIMIAK--AHWYLL 168
           E+     P    L DI  ++PK  +  N   +   V+  VA    GL  +A    +W L 
Sbjct: 79  ERFDPGAPPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVA-IVFGLAAVAAYFNNWLLW 138

Query: 169 PLAWAWTGTAITGATEVTFPFKFFVIGHDCAHKSFSRNKLVEDIVGTLAFMPLIYPYEPW 228
           PL W   GT           +  FV+GHDC H SFS +  +  + G L    ++ PY  W
Sbjct: 139 PLYWFAQGTMF---------WALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGW 198

Query: 229 RFKHDQHHAKTNMLKEDTAWHP----VWKEEFDSDPLLRKAIVYGY--GPFRPWMSI--A 288
           R  H  HH     ++ D +WHP    ++K    +  + R  + +     PF  W      
Sbjct: 199 RISHRTHHQNHGHVENDESWHPLPESIYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPGK 258

Query: 289 HWLMWHFDVKKFRPNEVKRVKISLACVFAFMFIGWPLIIYKAGIIGWVKFWLMPWLGYHF 348
               +H D   F P E K V  S AC  A   +    + +  G I  +K + +P+  +  
Sbjct: 259 QGSHYHPDSDLFLPKEKKDVLTSTACWTAMAAL-LVCLNFVMGPIQMLKLYGIPYWIFVM 318

Query: 349 WMSTFTMVHHTA--PHIPFKSSEEWNAAQAQLNGTVHCAYPKWIEILCHHINVHIPHHIS 405
           W+   T +HH      +P+   +EW+  +  L  T+   Y  WI  + H I  H+ HH+ 
Sbjct: 319 WLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLT-TLDRDY-GWINNIHHDIGTHVIHHLF 373