Cp4.1LG18g05210 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g05210
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionH(+)-exporting diphosphatase
LocationCp4.1LG18: 5942509 .. 5946770 (+)
RNA-Seq ExpressionCp4.1LG18g05210
SyntenyCp4.1LG18g05210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTCACGCGCGGAACACGCACATTCTATCCCCTTTTCTCTCTCACCGGGTTTTTTCACATACTATAAATACCAGCTCCCCTTTCTCCCAACACATCTCAGCCTCAAATCTTCGTTCATTCACCCACCGTGCGACCACCCCACCAAAACCCTTGTTTCTTCGTCCTTCTCCTTCGTTTCGTGGGGTTCGTGTCTGTGTGTGTTTCGCCATGGGCGTCGTCACCATTCTTCCAGATCTCGGCACTCAGATTTTCATCCCGGTCTGTGCCGTAGTAGGAATAGTCTTCTCTTTGGTGCAGTGGTACTATGTCTCTCAGGTTAAGCTGTCGCCTGGTCGAGATTCCACTCATAACAACTCCGCCGCTGCTAAAAATGGCTACTCCGACTACCTTATCGAGGAGGAAGAAGGAGTCAACGACCATAACGTTGTTATTAAGTGTGCCGAAATTCAGAATGCTATCTCTGAGGGTAAACCGCTTATCGTTATTGTTCATTTCAGATTTGTTTTCTGCTTTGTTCTTTATTAGGCCTTATTAATTGCTAGATCTGGTGATGGATTCTCAGATTTATGATTCTTCGATTTTGTTTTTCTCTTTTATGGATATTTTTTTGTCTTTTCCTTTTGTGAACGCTTTAGGTTTTATCTTCTTTTTCTCTCCCATGGGATTTCATTTCTCGTTTCTTGACTGGCTCTTAGTTTCCGGAGCGATTTTTCAGTTTGGATTCTTCTTAGTGAAGTAGATGTTTGGATGTCTGTTCACCGGTCTTACACCATTTTCATAAATACTTTACGGAGCCACTAATTACGGATTACAATTATTTGTTTTGATTGGTTATCGCGTCTGTAATACACGTAGTCTTTTGTCTATGAATTCTTTCAAGAATCTTCAAACCGATTATGATAACAAGGATTTGAAATTATGAAGAGATCTAGGAAATGATGGTTAAATAGTTTCAATTTATTAAAAATAGAAGAAGACGCACTGTCAGATTCTTTGGCACTTTCTCATATCTGGGTGTTGTTTTTTATCAGTTTGGGTTGTAATGTTCCTTTTTTTGGGAAGAGGATGAAGGTGCATCACCACATCTAGAGCTAATCAGCACTTCTTTTAAGTTCATGAGATTCCTGTTTTTTTGCATCGGGTTCTTCGTTAATATCTTGTTTGTTCCGTGTGATATTTATGGAATTGTTGTTTTTATATATGGTATGCAAATAAATTAATACTCTGTTTTTTTGGGTTAAAAATTGAAAATATTTCTATGAATTCCTTTTGTTGAAGTCTCTCTCTACGTTTTGATGTATTAGGGGCAACCTCCTTCCTGTTCACGGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTGTTCCTTGGCTCAGTGGAGGGTTTTAGTACCGAGCCTCAAGCGTGCACTTATGACAAAACAAAGATTTGCAAGCCCGCTTTGGCGACGGCTATATTCAGCACTATATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACGTCTTCAAGTTGTACTATGGCGAAGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTAACGACCACTGCTCTAGATATCTTTTCTTAATTCATTTTGTGCTGATACTTTTCTCTTTGAATCTAATCACTCGTTCTTTTGGCAGGTCATTGCTGATAATGTGGGTGACAATGTTGGGGATATTGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCTCTTGTTGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGCTGACACCAATGCTCTATCCACTCATAGTTAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCTACTGTTCTAATGACCTTTGGAATTGCCATTGTTACTTGGGTGGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTACAGAACTGGTTAGTGCTTCTTGAATTATTGTCTTACAATTGCTTTTTTCTATCACTATTGGTTTGATTCACCAGAAAATATTTGGCATTCAGGAAACTTTTCTTGTGCGTTGCTGTCGGTCTTTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACTAGCAATGCATACAGGTATTTGATTTTTGGGCATTTACCTAGTCAATTCTCAACGATTCAATTACTTCTGTATATAAGAACGAAAATCTGATCACGGATTTGGTTTGGGTTTTCAGCCCTGTGCAGGATGTTGCTGATTCCTGCCGAACCGGAGCTGCTACAAATGTTATTTTTGGCTTGGCTTTGGGATACAAATCTGTCATTATCCCCATTTTTGCAATTGCAGTTAGCATTTTTGTTAGTTTCACCTTTGCTGCAATGTACGGCATTGCTGTTGCTGCTCTTGGAATGTTGAGTACAATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCAGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTCGATGCTGCAGGAAACACAACTGCAGCCATTGGAAAGGTATTTTCATTTATTCTTTTCGTCTCAATTGTAATGTTTTCTTTATCACAATTTGTGCATGAGAAGCTATGCTTTCAGTCGGTCGTAGCTTATTCTTTATTTGCATTTTCCAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCCCTTGCTCTCTTCGGCGCCTTCGTCAGCCGTGCTGGTGTCACTTCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTAGGTGCTATGCTGCCGTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTCGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGCACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACACCCCTAATTGTCGGTATCCTATTCGGGGTCGAAACTCTTTCTGGCGTTCTTGCTGGATCCCTTGTTTCTGGTGTGCAGGTATATGTGATGGCCTGTTAAAACCTTTGCTTGAACCTTATAATGCCACCATCTAACTGATGGTTCTTGACGTGCAGATCGCCATCTCTGCATCCAACACTGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGTATGTCGAATCCAATAGTTATCTTTACAACGTTGTAACGATTTCATGGTACTAGATGTAATAGCAAGTCGTCTTATGGATAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCGAAAGGATCAGATCCACACAAAGCCGCTGTTATCGGCGACACGATCGGAGACCCGCTCAAGGATACCTCCGGACCTTCTCTCAACATTCTCATCAAGCTGATGGCTGTGGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCACTCACGGTGGGCTTCTCTTCAGGCTCTAGAAAAAGCAAGAGGAATCTTTGTTCGAATCCATTGACATAGCCTCTCTACCTCTCCTCCACGAAATTTTCACTGTGTTTTTTAGACTTGGTATTATATGTTAAAGTCTCTGTAGCTTTTGATTGATATTCCCTTTTGAGAAACTTGTAGTTTCAAGTGGAAGAGCCTTTTACCTTTTCATCGGTTGTTGATGAAGAAGAAGAAAAGAAGAGATATTAGTTAAATCTTTTGAAATGAGACTGCAATGTTATTCTCTTTTTCCCAATTGAGAACCCTTTTCATACTCTCATTTTCTTACTGATGTAGTTCTTGATGTAGTTCGGCGTTCACTATGTCAGTCGGAGAAGGGAACGAAATATTTCTTA

mRNA sequence

TGTTCACGCGCGGAACACGCACATTCTATCCCCTTTTCTCTCTCACCGGGTTTTTTCACATACTATAAATACCAGCTCCCCTTTCTCCCAACACATCTCAGCCTCAAATCTTCGTTCATTCACCCACCGTGCGACCACCCCACCAAAACCCTTGTTTCTTCGTCCTTCTCCTTCGTTTCGTGGGGTTCGTGTCTGTGTGTGTTTCGCCATGGGCGTCGTCACCATTCTTCCAGATCTCGGCACTCAGATTTTCATCCCGGTCTGTGCCGTAGTAGGAATAGTCTTCTCTTTGGTGCAGTGGTACTATGTCTCTCAGGTTAAGCTGTCGCCTGGTCGAGATTCCACTCATAACAACTCCGCCGCTGCTAAAAATGGCTACTCCGACTACCTTATCGAGGAGGAAGAAGGAGTCAACGACCATAACGTTGTTATTAAGTGTGCCGAAATTCAGAATGCTATCTCTGAGGGGGCAACCTCCTTCCTGTTCACGGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTGTTCCTTGGCTCAGTGGAGGGTTTTAGTACCGAGCCTCAAGCGTGCACTTATGACAAAACAAAGATTTGCAAGCCCGCTTTGGCGACGGCTATATTCAGCACTATATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACGTCTTCAAGTTGTACTATGGCGAAGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTCATTGCTGATAATGTGGGTGACAATGTTGGGGATATTGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCTCTTGTTGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGCTGACACCAATGCTCTATCCACTCATAGTTAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCTACTGTTCTAATGACCTTTGGAATTGCCATTGTTACTTGGGTGGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTACAGAACTGGAAACTTTTCTTGTGCGTTGCTGTCGGTCTTTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACTAGCAATGCATACAGCCCTGTGCAGGATGTTGCTGATTCCTGCCGAACCGGAGCTGCTACAAATGTTATTTTTGGCTTGGCTTTGGGATACAAATCTGTCATTATCCCCATTTTTGCAATTGCAGTTAGCATTTTTGTTAGTTTCACCTTTGCTGCAATGTACGGCATTGCTGTTGCTGCTCTTGGAATGTTGAGTACAATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCAGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTCGATGCTGCAGGAAACACAACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCCCTTGCTCTCTTCGGCGCCTTCGTCAGCCGTGCTGGTGTCACTTCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTAGGTGCTATGCTGCCGTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTCGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGCACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACACCCCTAATTGTCGGTATCCTATTCGGGGTCGAAACTCTTTCTGGCGTTCTTGCTGGATCCCTTGTTTCTGGTGTGCAGATCGCCATCTCTGCATCCAACACTGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCGAAAGGATCAGATCCACACAAAGCCGCTGTTATCGGCGACACGATCGGAGACCCGCTCAAGGATACCTCCGGACCTTCTCTCAACATTCTCATCAAGCTGATGGCTGTGGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCACTCACGGTGGGCTTCTCTTCAGGCTCTAGAAAAAGCAAGAGGAATCTTTGTTCGAATCCATTGACATAGCCTCTCTACCTCTCCTCCACGAAATTTTCACTGTGTTTTTTAGACTTGGTATTATATGTTAAAGTCTCTGTAGCTTTTGATTGATATTCCCTTTTGAGAAACTTGTAGTTTCAAGTGGAAGAGCCTTTTACCTTTTCATCGGTTGTTGATGAAGAAGAAGAAAAGAAGAGATATTAGTTAAATCTTTTGAAATGAGACTGCAATGTTATTCTCTTTTTCCCAATTGAGAACCCTTTTCATACTCTCATTTTCTTACTGATGTAGTTCTTGATGTAGTTCGGCGTTCACTATGTCAGTCGGAGAAGGGAACGAAATATTTCTTA

Coding sequence (CDS)

ATGGGCGTCGTCACCATTCTTCCAGATCTCGGCACTCAGATTTTCATCCCGGTCTGTGCCGTAGTAGGAATAGTCTTCTCTTTGGTGCAGTGGTACTATGTCTCTCAGGTTAAGCTGTCGCCTGGTCGAGATTCCACTCATAACAACTCCGCCGCTGCTAAAAATGGCTACTCCGACTACCTTATCGAGGAGGAAGAAGGAGTCAACGACCATAACGTTGTTATTAAGTGTGCCGAAATTCAGAATGCTATCTCTGAGGGGGCAACCTCCTTCCTGTTCACGGAGTACAAGTATGTTGGCATCTTTATGGTGTTGTTTGCAGTCTTGATTTTCGTGTTCCTTGGCTCAGTGGAGGGTTTTAGTACCGAGCCTCAAGCGTGCACTTATGACAAAACAAAGATTTGCAAGCCCGCTTTGGCGACGGCTATATTCAGCACTATATCATTTTTACTTGGTGCAGTTACTTCAGTGGTTTCTGGTTTCCTTGGAATGAAAATTGCTACATATGCAAATGCCAGAACCACCTTGGAGGCAAGAAAAGGTGTTGGAAAGGCATTTATTACTGCTTTTAGGTCTGGTGCTGTCATGGGCTTCCTCCTAGCTGCCAATGGTCTCTTGGTTTTGTTTATTGCCATTAACGTCTTCAAGTTGTACTATGGCGAAGATTGGGGTGGCCTTTTTGAGTCTATCACTGGGTATGGTCTTGGTGGATCTTCCATGGCTCTCTTTGGTAGAGTTGGTGGTGGTATCTACACTAAAGCTGCTGATGTTGGTGCTGACCTTGTGGGCAAGGTGGAAAGGAACATTCCTGAGGATGATCCAAGAAACCCAGCTGTCATTGCTGATAATGTGGGTGACAATGTTGGGGATATTGCTGGTATGGGATCTGATCTTTTTGGTTCATATGCTGAATCATCCTGTGCTGCTCTTGTTGTTGCTTCTATCTCTTCTTTCGGCAACAACCATGAGCTGACACCAATGCTCTATCCACTCATAGTTAGTTCTGTGGGTATCCTTGTTTGCCTGATCACCACCTTATTTGCTACTGATTTCTTTGAGATCAAGGCTGTTAAGGAAATTGAGCCAGCATTGAAGAAGCAACTCATAATTTCTACTGTTCTAATGACCTTTGGAATTGCCATTGTTACTTGGGTGGCTGTTCCATCAACATTCACCATTTTCAATTTTGGAACTCAAAAAGTTGTACAGAACTGGAAACTTTTCTTGTGCGTTGCTGTCGGTCTTTGGGCTGGGCTTATAATAGGATTTGTGACAGAGTACTATACTAGCAATGCATACAGCCCTGTGCAGGATGTTGCTGATTCCTGCCGAACCGGAGCTGCTACAAATGTTATTTTTGGCTTGGCTTTGGGATACAAATCTGTCATTATCCCCATTTTTGCAATTGCAGTTAGCATTTTTGTTAGTTTCACCTTTGCTGCAATGTACGGCATTGCTGTTGCTGCTCTTGGAATGTTGAGTACAATAGCTACTGGTTTGGCCATTGATGCATATGGTCCAATCAGTGACAATGCTGGAGGCATTGCAGAGATGGCAGGCATGAGCCACAGAATTCGGGAGAGAACCGATGCCCTCGATGCTGCAGGAAACACAACTGCAGCCATTGGAAAGGGTTTTGCCATTGGTTCAGCTGCTCTTGTGTCCCTTGCTCTCTTCGGCGCCTTCGTCAGCCGTGCTGGTGTCACTTCTGTTGACGTCTTGACACCCAAAGTTTTCATTGGCTTGATCGTAGGTGCTATGCTGCCGTACTGGTTTTCTGCCATGACAATGAAGAGTGTTGGGAGTGCAGCCCTAAAGATGGTCGAGGAGGTGCGAAGGCAATTCAACACCATCCCAGGTCTCATGGAGGGCACTGCCAAGCCCGACTACGCTACATGTGTCAAGATCTCAACTGATGCTTCTATCAAGGAGATGATCCCACCTGGTGCCCTTGTCATGTTGACACCCCTAATTGTCGGTATCCTATTCGGGGTCGAAACTCTTTCTGGCGTTCTTGCTGGATCCCTTGTTTCTGGTGTGCAGATCGCCATCTCTGCATCCAACACTGGAGGTGCCTGGGATAACGCTAAGAAGTACATCGAGGCTGGTGCCTCTGAGCATGCAAGAACCCTCGGCCCGAAAGGATCAGATCCACACAAAGCCGCTGTTATCGGCGACACGATCGGAGACCCGCTCAAGGATACCTCCGGACCTTCTCTCAACATTCTCATCAAGCTGATGGCTGTGGAGTCCCTTGTGTTTGCTCCTTTCTTTGCCACTCACGGTGGGCTTCTCTTCAGGCTCTAG

Protein sequence

MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL
Homology
BLAST of Cp4.1LG18g05210 vs. ExPASy Swiss-Prot
Match: P21616 (Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radiata OX=3916 PE=1 SV=4)

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 704/763 (92.27%), Postives = 742/763 (97.25%), Query Frame = 0

Query: 6   ILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIEEE 65
           ILPDLGT+I IPVCAV+GI F+L QW  VS+VKLS  RD++ N  AAAKNGY+DYLIEEE
Sbjct: 5   ILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPN--AAAKNGYNDYLIEEE 64

Query: 66  EGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEPQ 125
           EG+NDHNVV+KCAEIQNAISEGATSFLFTEYKYVGIFMV FA+LIF+FLGSVEGFST PQ
Sbjct: 65  EGINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQ 124

Query: 126 ACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKA 185
           AC+YDKTK CKPALATAIFST+SFLLG VTS+VSGFLGMKIATYANARTTLEARKGVGKA
Sbjct: 125 ACSYDKTKTCKPALATAIFSTVSFLLGGVTSLVSGFLGMKIATYANARTTLEARKGVGKA 184

Query: 186 FITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFGR 245
           FITAFRSGAVMGFLLAANGLLVL+IAIN+FK+YYG+DWGGLFE+ITGYGLGGSSMALFGR
Sbjct: 185 FITAFRSGAVMGFLLAANGLLVLYIAINLFKIYYGDDWGGLFEAITGYGLGGSSMALFGR 244

Query: 246 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 305
           VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES
Sbjct: 245 VGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAES 304

Query: 306 SCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALK 365
           SCAALVVASISSFG NHELT MLYPLIVSSVGILVCL+TTLFATDFFEIKAVKEIEPALK
Sbjct: 305 SCAALVVASISSFGLNHELTAMLYPLIVSSVGILVCLLTTLFATDFFEIKAVKEIEPALK 364

Query: 366 KQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFVT 425
           KQL+ISTVLMT G+A+V++VA+P++FTIFNFG QK V++W+LFLCVAVGLWAGLIIGFVT
Sbjct: 365 KQLVISTVLMTIGVAVVSFVALPTSFTIFNFGVQKDVKSWQLFLCVAVGLWAGLIIGFVT 424

Query: 426 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGI 485
           EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSFTFAAMYGI
Sbjct: 425 EYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFTFAAMYGI 484

Query: 486 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 545
           AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG
Sbjct: 485 AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKG 544

Query: 546 FAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 605
           FAIGSAALVSLALFGAFVSRA +T+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL
Sbjct: 545 FAIGSAALVSLALFGAFVSRASITTVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAAL 604

Query: 606 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGV 665
           KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPL+VGILFGV
Sbjct: 605 KMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLVVGILFGV 664

Query: 666 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIG 725
           ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHAR+LGPKGSD HKAAVIG
Sbjct: 665 ETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARSLGPKGSDCHKAAVIG 724

Query: 726 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 769
           DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF++
Sbjct: 725 DTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKI 765

BLAST of Cp4.1LG18g05210 vs. ExPASy Swiss-Prot
Match: P31414 (Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana OX=3702 GN=AVP1 PE=1 SV=1)

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 669/763 (87.68%), Postives = 719/763 (94.23%), Query Frame = 0

Query: 6   ILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSA-AAKNGYSDYLIEE 65
           +LP+L T+I +P+CAV+GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 66  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 125
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVEGFST+ 
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 126 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 185
           + CTYD T+ CKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 186 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 245
           AFI AFRSGAVMGFLLAA+GLLVL+I INVFK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 246 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 306 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 365
           +SCAALVVASISSFG NH+ T M YPL++SS+GILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 366 KKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFV 425
           K QLIISTV+MT GIAIV+WV +P++FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 485
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 486 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 546 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 605
           GFAIGSAALVSLALFGAFVSRAG+ +VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 665
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 725
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFR 768
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGG+LF+
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of Cp4.1LG18g05210 vs. ExPASy Swiss-Prot
Match: Q06572 (Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513 PE=2 SV=2)

HSP 1 Score: 1266.5 bits (3276), Expect = 0.0e+00
Identity = 667/764 (87.30%), Postives = 717/764 (93.85%), Query Frame = 0

Query: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63
           + IL +LGT+I IPVC V+GIVF++ QW+ VS+VK++PG  S    +A AKNGY DYLIE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASA---AAGAKNGYGDYLIE 60

Query: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123
           EEEG+NDHNVV+KCAEIQ AISEGATSFLFT Y+YVG+FMV+FA +IF+FLGS+EGFST+
Sbjct: 61  EEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTK 120

Query: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183
            Q CTY K   CKPAL TA+FST SFLLGA+TS+VSGFLGMKIATYANARTTLEARKGVG
Sbjct: 121 GQPCTYSK-GTCKPALYTALFSTASFLLGAITSLVSGFLGMKIATYANARTTLEARKGVG 180

Query: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243
           KAFITAFRSGAVMGFLL+++GL+VL+I INVFK+YYG+DW GLFESITGYGLGGSSMALF
Sbjct: 181 KAFITAFRSGAVMGFLLSSSGLVVLYITINVFKMYYGDDWEGLFESITGYGLGGSSMALF 240

Query: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 241 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 300

Query: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363
           ESSCAALVVASISSFG NH+ T M YPL+VSSVGI+VCL+TTLFATDFFEIKA  EIEPA
Sbjct: 301 ESSCAALVVASISSFGINHDFTAMCYPLLVSSVGIIVCLLTTLFATDFFEIKAANEIEPA 360

Query: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423
           LKKQLIIST LMT G+A+++W+A+P+ FTIFNFG QK V NW LF CVAVGLWAGLIIGF
Sbjct: 361 LKKQLIISTALMTVGVAVISWLALPAKFTIFNFGAQKEVSNWGLFFCVAVGLWAGLIIGF 420

Query: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSI+VSF+ AAMY
Sbjct: 421 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIYVSFSIAAMY 480

Query: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543
           GIA+AALGMLST+ATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 481 GIAMAALGMLSTMATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 540

Query: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603
           KGFAIGSAALVSLALFGAFVSRAGV  VDVL+PKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 541 KGFAIGSAALVSLALFGAFVSRAGVKVVDVLSPKVFIGLIVGAMLPYWFSAMTMKSVGSA 600

Query: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG LF
Sbjct: 601 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGTLF 660

Query: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723
           GVETLSGVLAG+LVSGVQIAISASNTGGAWDNAKKYIEAG SEHAR+LGPKGSD HKAAV
Sbjct: 661 GVETLSGVLAGALVSGVQIAISASNTGGAWDNAKKYIEAGNSEHARSLGPKGSDCHKAAV 720

Query: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFR 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFAT+GGLLF+
Sbjct: 721 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATYGGLLFK 760

BLAST of Cp4.1LG18g05210 vs. ExPASy Swiss-Prot
Match: Q72Q29 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130) OX=267671 GN=hppA PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 3.5e-199
Identity = 394/696 (56.61%), Postives = 505/696 (72.56%), Query Frame = 0

Query: 76  KCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGS--VEGFSTEPQACTYDKTK 135
           K  EI +AISEGA +FL  EYK + +F+   AVLI + L +   EGF+            
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN------------ 101

Query: 136 ICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSG 195
                    I++ I+F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SG
Sbjct: 102 -------DGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSG 161

Query: 196 AVMGFLLAANGLLVLFIAINVFK-LYYGEDWGGLFESITGYGLGGSSMALFGRVGGGIYT 255
           AVMGF L    +L + +   VF  +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 AVMGFGLVGLAILGMIVLFLVFTGMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 256 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 315
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 316 ASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 375
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 376 VLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 435
           +L+   +  VT   +  +F I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 436 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 495
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 496 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 555
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 556 LVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 615
           L SLALF AF++R   TS++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 616 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 675
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 676 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 735
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 736 KDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 769
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+F++
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of Cp4.1LG18g05210 vs. ExPASy Swiss-Prot
Match: Q8F641 (Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601) OX=189518 GN=hppA PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 3.5e-199
Identity = 394/696 (56.61%), Postives = 505/696 (72.56%), Query Frame = 0

Query: 76  KCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGS--VEGFSTEPQACTYDKTK 135
           K  EI +AISEGA +FL  EYK + +F+   AVLI + L +   EGF+            
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN------------ 101

Query: 136 ICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSG 195
                    I++ I+F+ GA+ S +SGF+GMKIAT  N RT   A+  + KAF  AF SG
Sbjct: 102 -------DGIYTAIAFVSGALISCISGFIGMKIATAGNVRTAEAAKSSMAKAFRVAFDSG 161

Query: 196 AVMGFLLAANGLLVLFIAINVFK-LYYGEDWGGLFESITGYGLGGSSMALFGRVGGGIYT 255
           AVMGF L    +L + +   VF  +Y G +   L ES+ G+GLGGS++ALFGRVGGGIYT
Sbjct: 162 AVMGFGLVGLAILGMIVLFLVFTGMYPGVEKHFLMESLAGFGLGGSAVALFGRVGGGIYT 221

Query: 256 KAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALVV 315
           KAADVGADLVGKVE+ IPEDDPRNPA IADNVGDNVGD+AGMG+DLFGS AE++CAALV+
Sbjct: 222 KAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGDVAGMGADLFGSCAEATCAALVI 281

Query: 316 ASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALKKQLIIST 375
            + +S   +  +  +LYPL++S+ GI   ++T+  A     +K    +E ALK QL +ST
Sbjct: 282 GATAS-ALSGSVDALLYPLLISAFGIPASILTSFLA----RVKEDGNVESALKVQLWVST 341

Query: 376 VLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFVTEYYTSNA 435
           +L+   +  VT   +  +F I      K +  W +++ + VGL++G+ IG VTEYYTS++
Sbjct: 342 LLVAGIMYFVTKTFMVDSFEI----AGKTITKWDVYISMVVGLFSGMFIGIVTEYYTSHS 401

Query: 436 YSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYGIAVAALGM 495
           Y PV++VA++  TGAATN+I+GL+LGY S +IP+  + ++I  +   A MYGIA+AALGM
Sbjct: 402 YKPVREVAEASNTGAATNIIYGLSLGYHSSVIPVILLVITIVTANLLAGMYGIAIAALGM 461

Query: 496 LSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGKGFAIGSAA 555
           +STIA GL IDAYGP+SDNAGGIAEMA +   +R+RTD LDAAGNTTAAIGKGFAIGSAA
Sbjct: 462 ISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRDRTDTLDAAGNTTAAIGKGFAIGSAA 521

Query: 556 LVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVR 615
           L SLALF AF++R   TS++VL  +VF GL+ GAMLP+ F+AMTMKSVG AA+ MVEEVR
Sbjct: 522 LTSLALFAAFITRTHTTSLEVLNAEVFGGLMFGAMLPFLFTAMTMKSVGKAAVDMVEEVR 581

Query: 616 RQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFGVETLSGVL 675
           +QF  IPG+MEG  KPDY  CV IST A+++EMI PG LV+LTP++VG LFGV+TL+GVL
Sbjct: 582 KQFKEIPGIMEGKNKPDYKRCVDISTSAALREMILPGLLVLLTPILVGYLFGVKTLAGVL 641

Query: 676 AGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPL 735
           AG+LV+GV +AISA+N+GG WDNAKKYIE  A       G KGSD HKAAV+GDT+GDP 
Sbjct: 642 AGALVAGVVLAISAANSGGGWDNAKKYIEKKA-------GGKGSDQHKAAVVGDTVGDPF 701

Query: 736 KDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 769
           KDTSGPS+NILIKLMA+ SLVFA FF   GGL+F++
Sbjct: 702 KDTSGPSINILIKLMAITSLVFAEFFVQQGGLIFKI 702

BLAST of Cp4.1LG18g05210 vs. NCBI nr
Match: XP_023515840.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1441 bits (3729), Expect = 0.0
Identity = 768/768 (100.00%), Postives = 768/768 (100.00%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY
Sbjct: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI
Sbjct: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL
Sbjct: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768

BLAST of Cp4.1LG18g05210 vs. NCBI nr
Match: KAG7023485.1 (hypothetical protein SDJN02_14510, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1436 bits (3717), Expect = 0.0
Identity = 765/768 (99.61%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSP RDSTHNNSAAAKNGYSDY
Sbjct: 39  MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDY 98

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 99  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 158

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 159 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 218

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 219 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 278

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 279 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 338

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 339 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 398

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIAIVTW+AVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI
Sbjct: 399 EPALKKQLIISTVLMTFGIAIVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 458

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 459 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 518

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 519 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 578

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 579 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 638

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 639 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 698

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 699 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 758

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF+L
Sbjct: 759 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 806

BLAST of Cp4.1LG18g05210 vs. NCBI nr
Match: XP_022960721.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita moschata] >KAG6589814.1 hypothetical protein SDJN03_15237, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1436 bits (3716), Expect = 0.0
Identity = 764/768 (99.48%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSP RDSTHNNSAAAKNGYSDY
Sbjct: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDY 60

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIA+VTW+AVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI
Sbjct: 361 EPALKKQLIISTVLMTFGIAVVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF+L
Sbjct: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of Cp4.1LG18g05210 vs. NCBI nr
Match: XP_022987612.1 (pyrophosphate-energized vacuolar membrane proton pump [Cucurbita maxima])

HSP 1 Score: 1435 bits (3714), Expect = 0.0
Identity = 764/768 (99.48%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSP RDSTHNNSAAAKNGYSDY
Sbjct: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDY 60

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIAIVTW+AVPSTFTIFNFGTQKVVQNWKLFLCV+VGLWAGLI
Sbjct: 361 EPALKKQLIISTVLMTFGIAIVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVSVGLWAGLI 420

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF+L
Sbjct: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of Cp4.1LG18g05210 vs. NCBI nr
Match: XP_022929683.1 (pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] >KAG6587868.1 hypothetical protein SDJN03_16433, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021755.1 hypothetical protein SDJN02_15482 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1402 bits (3630), Expect = 0.0
Identity = 742/765 (96.99%), Postives = 759/765 (99.22%), Query Frame = 0

Query: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63
           VTILPDLGT+IFIPVCAVVGIVFSLVQWYYVSQVKLSPGRD+ HNNSA +KNGYSDYLIE
Sbjct: 3   VTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62

Query: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123
           EEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+
Sbjct: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122

Query: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183
           PQ CTYDKT+ CKPALATAIFST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182

Query: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242

Query: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302

Query: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363
           ESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPA
Sbjct: 303 ESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362

Query: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423
           LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLIIGF
Sbjct: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422

Query: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY
Sbjct: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482

Query: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542

Query: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602

Query: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662

Query: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722

Query: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Cp4.1LG18g05210 vs. ExPASy TrEMBL
Match: A0A6J1H8E2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 SV=1)

HSP 1 Score: 1436 bits (3716), Expect = 0.0
Identity = 764/768 (99.48%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSP RDSTHNNSAAAKNGYSDY
Sbjct: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDY 60

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIA+VTW+AVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI
Sbjct: 361 EPALKKQLIISTVLMTFGIAVVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF+L
Sbjct: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of Cp4.1LG18g05210 vs. ExPASy TrEMBL
Match: A0A6J1JAU2 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111485119 PE=3 SV=1)

HSP 1 Score: 1435 bits (3714), Expect = 0.0
Identity = 764/768 (99.48%), Postives = 767/768 (99.87%), Query Frame = 0

Query: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDY 60
           MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSP RDSTHNNSAAAKNGYSDY
Sbjct: 1   MGVVTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPSRDSTHNNSAAAKNGYSDY 60

Query: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120
           LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF
Sbjct: 61  LIEEEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGF 120

Query: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180
           STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK
Sbjct: 121 STEPQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARK 180

Query: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240
           GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM
Sbjct: 181 GVGKAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSM 240

Query: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300
           ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG
Sbjct: 241 ALFGRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFG 300

Query: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360
           SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI
Sbjct: 301 SYAESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEI 360

Query: 361 EPALKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLI 420
           EPALKKQLIISTVLMTFGIAIVTW+AVPSTFTIFNFGTQKVVQNWKLFLCV+VGLWAGLI
Sbjct: 361 EPALKKQLIISTVLMTFGIAIVTWLAVPSTFTIFNFGTQKVVQNWKLFLCVSVGLWAGLI 420

Query: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480
           IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA
Sbjct: 421 IGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFA 480

Query: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540
           AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA
Sbjct: 481 AMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTA 540

Query: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600
           AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV
Sbjct: 541 AIGKGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSV 600

Query: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660
           GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG
Sbjct: 601 GSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVG 660

Query: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720
           ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK
Sbjct: 661 ILFGVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHK 720

Query: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLF+L
Sbjct: 721 AAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFKL 768

BLAST of Cp4.1LG18g05210 vs. ExPASy TrEMBL
Match: A0A6J1ESX2 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 SV=1)

HSP 1 Score: 1402 bits (3630), Expect = 0.0
Identity = 742/765 (96.99%), Postives = 759/765 (99.22%), Query Frame = 0

Query: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63
           VTILPDLGT+IFIPVCAVVGIVFSLVQWYYVSQVKLSPGRD+ HNNSA +KNGYSDYLIE
Sbjct: 3   VTILPDLGTEIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62

Query: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123
           EEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+
Sbjct: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122

Query: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183
           PQ CTYDKT+ CKPALATAIFST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182

Query: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242

Query: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302

Query: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363
           ESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPA
Sbjct: 303 ESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362

Query: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423
           LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLIIGF
Sbjct: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422

Query: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY
Sbjct: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482

Query: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542

Query: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602

Query: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662

Query: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722

Query: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Cp4.1LG18g05210 vs. ExPASy TrEMBL
Match: A0A6J1KSL0 (H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV=1)

HSP 1 Score: 1402 bits (3628), Expect = 0.0
Identity = 740/765 (96.73%), Postives = 759/765 (99.22%), Query Frame = 0

Query: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63
           VTILPDLGT+IFIPVCAV+GI+FSLVQWYYVSQVKLSPGRD+ HNNSA +KNGYSDYLIE
Sbjct: 3   VTILPDLGTEIFIPVCAVIGIIFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62

Query: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123
           EEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+
Sbjct: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122

Query: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183
           PQ CTYDKT+ CKPALATAIFST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182

Query: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242

Query: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302

Query: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363
           ESSCAALVVASISSFGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPA
Sbjct: 303 ESSCAALVVASISSFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362

Query: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423
           LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLIIGF
Sbjct: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422

Query: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY
Sbjct: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482

Query: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542

Query: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602

Query: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662

Query: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV
Sbjct: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 722

Query: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Cp4.1LG18g05210 vs. ExPASy TrEMBL
Match: O82680 (H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1)

HSP 1 Score: 1399 bits (3620), Expect = 0.0
Identity = 739/765 (96.60%), Postives = 758/765 (99.08%), Query Frame = 0

Query: 4   VTILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSAAAKNGYSDYLIE 63
           VTILPDLGT+IFIPVCAV+GIVFSLVQWYYVSQVKLSPGRD+ HNNSA +KNGYSDYLIE
Sbjct: 3   VTILPDLGTEIFIPVCAVIGIVFSLVQWYYVSQVKLSPGRDAAHNNSAGSKNGYSDYLIE 62

Query: 64  EEEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTE 123
           EEEGVNDHNVVIKCAEIQ+AISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVE FST+
Sbjct: 63  EEEGVNDHNVVIKCAEIQSAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVESFSTK 122

Query: 124 PQACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 183
           PQ CTYDKT+ CKPALATAIFST+SFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG
Sbjct: 123 PQPCTYDKTRTCKPALATAIFSTVSFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVG 182

Query: 184 KAFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALF 243
           KAFITAFRSGAVMGFLLAANGLLVLFIAIN+FKLYYG+DWGGLFESITGYGLGGSSMALF
Sbjct: 183 KAFITAFRSGAVMGFLLAANGLLVLFIAINLFKLYYGDDWGGLFESITGYGLGGSSMALF 242

Query: 244 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 303
           GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA
Sbjct: 243 GRVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYA 302

Query: 304 ESSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPA 363
           ESSCAALVVASIS FGNNHELTPMLYPLIVSS+GILVCLITTLFATDFFEIKAVKEIEPA
Sbjct: 303 ESSCAALVVASISPFGNNHELTPMLYPLIVSSMGILVCLITTLFATDFFEIKAVKEIEPA 362

Query: 364 LKKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGF 423
           LKKQLIISTVLMTFGIAIVTW++VPS+FTIFNFGTQKVV NWKLFLCVAVGLWAGLIIGF
Sbjct: 363 LKKQLIISTVLMTFGIAIVTWLSVPSSFTIFNFGTQKVVTNWKLFLCVAVGLWAGLIIGF 422

Query: 424 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 483
           VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY
Sbjct: 423 VTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMY 482

Query: 484 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 543
           GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG
Sbjct: 483 GIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIG 542

Query: 544 KGFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 603
           KGFAIGSAALVSLALFGAFVSRAGVT+VDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA
Sbjct: 543 KGFAIGSAALVSLALFGAFVSRAGVTAVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSA 602

Query: 604 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 663
           ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF
Sbjct: 603 ALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILF 662

Query: 664 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAV 723
           GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGAS+HARTLGPKGSDPHKAAV
Sbjct: 663 GVETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASKHARTLGPKGSDPHKAAV 722

Query: 724 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFRL 768
           IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFA+HGGLLF++
Sbjct: 723 IGDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFASHGGLLFKI 767

BLAST of Cp4.1LG18g05210 vs. TAIR 10
Match: AT1G15690.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1281.5 bits (3315), Expect = 0.0e+00
Identity = 669/763 (87.68%), Postives = 719/763 (94.23%), Query Frame = 0

Query: 6   ILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSA-AAKNGYSDYLIEE 65
           +LP+L T+I +P+CAV+GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 66  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 125
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVEGFST+ 
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 126 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 185
           + CTYD T+ CKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 186 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 245
           AFI AFRSGAVMGFLLAA+GLLVL+I INVFK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 246 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 306 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 365
           +SCAALVVASISSFG NH+ T M YPL++SS+GILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 366 KKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFV 425
           K QLIISTV+MT GIAIV+WV +P++FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 485
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 486 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 546 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 605
           GFAIGSAALVSLALFGAFVSRAG+ +VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGALVMLTPLIVGILFG 665
           LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPG LVMLTPLIVG  FG
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPPGCLVMLTPLIVGFFFG 665

Query: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVI 725
           VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAG SEHA++LGPKGS+PHKAAVI
Sbjct: 666 VETLSGVLAGSLVSGVQIAISASNTGGAWDNAKKYIEAGVSEHAKSLGPKGSEPHKAAVI 725

Query: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGLLFR 768
           GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGG+LF+
Sbjct: 726 GDTIGDPLKDTSGPSLNILIKLMAVESLVFAPFFATHGGILFK 768

BLAST of Cp4.1LG18g05210 vs. TAIR 10
Match: AT1G15690.2 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 1039.3 bits (2686), Expect = 1.6e-303
Identity = 543/628 (86.46%), Postives = 588/628 (93.63%), Query Frame = 0

Query: 6   ILPDLGTQIFIPVCAVVGIVFSLVQWYYVSQVKLSPGRDSTHNNSA-AAKNGYSDYLIEE 65
           +LP+L T+I +P+CAV+GI FSL QWY VS+VKL+    ++ +  A   KNGY DYLIEE
Sbjct: 6   LLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGDYLIEE 65

Query: 66  EEGVNDHNVVIKCAEIQNAISEGATSFLFTEYKYVGIFMVLFAVLIFVFLGSVEGFSTEP 125
           EEGVND +VV KCAEIQ AISEGATSFLFTEYKYVG+FM+ FA +IFVFLGSVEGFST+ 
Sbjct: 66  EEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEGFSTDN 125

Query: 126 QACTYDKTKICKPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGK 185
           + CTYD T+ CKPALATA FSTI+F+LGAVTSV+SGFLGMKIATYANARTTLEARKGVGK
Sbjct: 126 KPCTYDTTRTCKPALATAAFSTIAFVLGAVTSVLSGFLGMKIATYANARTTLEARKGVGK 185

Query: 186 AFITAFRSGAVMGFLLAANGLLVLFIAINVFKLYYGEDWGGLFESITGYGLGGSSMALFG 245
           AFI AFRSGAVMGFLLAA+GLLVL+I INVFK+YYG+DW GLFE+ITGYGLGGSSMALFG
Sbjct: 186 AFIVAFRSGAVMGFLLAASGLLVLYITINVFKIYYGDDWEGLFEAITGYGLGGSSMALFG 245

Query: 246 RVGGGIYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305
           RVGGGIYTKAADVGADLVGK+ERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE
Sbjct: 246 RVGGGIYTKAADVGADLVGKIERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAE 305

Query: 306 SSCAALVVASISSFGNNHELTPMLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPAL 365
           +SCAALVVASISSFG NH+ T M YPL++SS+GILVCLITTLFATDFFEIK VKEIEPAL
Sbjct: 306 ASCAALVVASISSFGINHDFTAMCYPLLISSMGILVCLITTLFATDFFEIKLVKEIEPAL 365

Query: 366 KKQLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLFLCVAVGLWAGLIIGFV 425
           K QLIISTV+MT GIAIV+WV +P++FTIFNFGTQKVV+NW+LFLCV VGLWAGLIIGFV
Sbjct: 366 KNQLIISTVIMTVGIAIVSWVGLPTSFTIFNFGTQKVVKNWQLFLCVCVGLWAGLIIGFV 425

Query: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTFAAMYG 485
           TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIA+SIFVSF+FAAMYG
Sbjct: 426 TEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAISIFVSFSFAAMYG 485

Query: 486 IAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545
           +AVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK
Sbjct: 486 VAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDAAGNTTAAIGK 545

Query: 546 GFAIGSAALVSLALFGAFVSRAGVTSVDVLTPKVFIGLIVGAMLPYWFSAMTMKSVGSAA 605
           GFAIGSAALVSLALFGAFVSRAG+ +VDVLTPKV IGL+VGAMLPYWFSAMTMKSVGSAA
Sbjct: 546 GFAIGSAALVSLALFGAFVSRAGIHTVDVLTPKVIIGLLVGAMLPYWFSAMTMKSVGSAA 605

Query: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 633
           LKMVEEVRRQFNTIPGLMEGTAKPDYAT
Sbjct: 606 LKMVEEVRRQFNTIPGLMEGTAKPDYAT 633

BLAST of Cp4.1LG18g05210 vs. TAIR 10
Match: AT1G16780.1 (Inorganic H pyrophosphatase family protein )

HSP 1 Score: 423.3 bits (1087), Expect = 4.1e-118
Identity = 288/734 (39.24%), Postives = 418/734 (56.95%), Query Frame = 0

Query: 79  EIQNAISEGATSFLFTEYKYVG--IFMVLFAVL-IFVFLGSVEGFSTEPQACTYDKTKIC 138
           +I +AI +GA  FL T+Y  +    F++ F +L I++F       + + +A    +T   
Sbjct: 150 QISDAIRDGAEGFLRTQYGTISKMAFLLAFVILCIYLF----RNLTPQQEASGLGRT--- 209

Query: 139 KPALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAV 198
                +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G  
Sbjct: 210 ----MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGF 269

Query: 199 MGFLLAANGLLVLFIAINVFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGG 258
              ++    ++ + I  + F ++   D  G  +       + GYG G S +ALF ++GGG
Sbjct: 270 SALVVVGMAVIGIAILYSTFYVWLDVDSPGSMKVTDLPLLLVGYGFGASFVALFAQLGGG 329

Query: 259 IYTKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAA 318
           IYTK ADVGADLVGKVE  IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A
Sbjct: 330 IYTKGADVGADLVGKVEHGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISA 389

Query: 319 LVVASISSFGNNHE------LTPML---YPLIVSSVGILVCLITTLFATDFFEIKA-VKE 378
           +++    +     E      L P++   + L++SS+GIL     ++  T    +K+ V++
Sbjct: 390 MILGGTMAQKCKIEDPSGFILFPLVVHSFDLVISSIGIL-----SIKGTRNASVKSPVED 449

Query: 379 IEPALKK--QLIISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNW-KLFLCVAVGLW 438
               L+K   L I   ++TFG A   W+            T++    W   F+C  VG+ 
Sbjct: 450 PMVVLQKGYSLTIILAVLTFG-ASTRWLLY----------TEQAPSAWLNFFMCGLVGII 509

Query: 439 AGLIIGFVTEYYTSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVS 498
              +  +++ YYT   Y PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  +
Sbjct: 510 TAYVFVWISRYYTDYKYEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLVISVAIISA 569

Query: 499 FTF--------------AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMS 558
           F                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+   
Sbjct: 570 FWLGNTSGLIDEKGNPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQP 629

Query: 559 HRIRERTDALDAAGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGVTSVDVLTP 618
             +RE TD LDA GNTT A  KGFAIGSAAL S  LF A++      +      VD+  P
Sbjct: 630 ESVREITDVLDAVGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIP 689

Query: 619 KVFIGLIVGAMLPYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKI 678
           +VFIG ++GAML + FSA    +VG  A ++V EVRRQF   PG+M+   KPDY  CV I
Sbjct: 690 EVFIGGLLGAMLIFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMDYKEKPDYGRCVAI 749

Query: 679 STDASIKEMIPPGALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAI 738
              ++++EMI PGAL +++P+ VG +F            G + ++ +L  + V G+ +A+
Sbjct: 750 VASSALREMIKPGALAIISPIAVGFVFRILGYYTGQPLLGAKVVAAMLMFATVCGILMAL 809

Query: 739 SASNTGGAWDNAKKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILI 759
             +  GGAWDNAKKYIE GA      LG KGSD HKAAV GDT+GDP KDT+GPS+++LI
Sbjct: 810 FLNTAGGAWDNAKKYIETGA------LGGKGSDSHKAAVTGDTVGDPFKDTAGPSIHVLI 850

BLAST of Cp4.1LG18g05210 vs. TAIR 10
Match: AT1G78920.1 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 419.5 bits (1077), Expect = 5.9e-117
Identity = 278/722 (38.50%), Postives = 410/722 (56.79%), Query Frame = 0

Query: 79  EIQNAISEGATSFLFTEYKYVGIFMVLFA-VLIFVFLGSVEGFSTEPQACTYDKTKICKP 138
           EI +AI +GA  F  T+Y  +    +L A V++ ++L      + + +A    +      
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYL--FRSLTPQQEAAGLGRA----- 160

Query: 139 ALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMG 198
              +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G    
Sbjct: 161 --MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSA 220

Query: 199 FLLAANGLLVLFIAINVFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGGIY 258
            ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIY
Sbjct: 221 LVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIY 280

Query: 259 TKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALV 318
           TK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A++
Sbjct: 281 TKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMI 340

Query: 319 VASISSFGNNHELTP--MLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALKKQLI 378
           +    +     E     +L+PL+V S  +++  I  L       IK  ++       +  
Sbjct: 341 LGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL------SIKGTRDASVKSPVEDP 400

Query: 379 ISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLF-LCVAVGLWAGLIIGFVTEYY 438
           ++ +   + + I+  V      T +   T++    W  F LC  VG+    I  ++++YY
Sbjct: 401 MAVLQKGYSLTIILAVITFGASTRWLLYTEQAPSAWFNFALCGLVGIITAYIFVWISKYY 460

Query: 439 TSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF--------- 498
           T   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++           
Sbjct: 461 TDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLVDE 520

Query: 499 -----AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDA 558
                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD LDA
Sbjct: 521 NGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLLDA 580

Query: 559 AGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGVTSVDVLTPKVFIGLIVGAML 618
            GNTT A  KGFAIGSAAL S  LF A++      +      VD+  P+VF+G ++GAML
Sbjct: 581 VGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGAML 640

Query: 619 PYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPP 678
            + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++EMI P
Sbjct: 641 IFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMIKP 700

Query: 679 GALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGAWDNA 738
           GAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGAWDNA
Sbjct: 701 GALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWDNA 760

Query: 739 KKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAP 759
           KKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +LV AP
Sbjct: 761 KKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVMAP 801

BLAST of Cp4.1LG18g05210 vs. TAIR 10
Match: AT1G78920.2 (vacuolar H+-pyrophosphatase 2 )

HSP 1 Score: 419.5 bits (1077), Expect = 5.9e-117
Identity = 278/722 (38.50%), Postives = 410/722 (56.79%), Query Frame = 0

Query: 79  EIQNAISEGATSFLFTEYKYVGIFMVLFA-VLIFVFLGSVEGFSTEPQACTYDKTKICKP 138
           EI +AI +GA  F  T+Y  +    +L A V++ ++L      + + +A    +      
Sbjct: 101 EISDAIRDGAEGFFRTQYSTISKMAILLAFVILCIYL--FRSLTPQQEAAGLGRA----- 160

Query: 139 ALATAIFSTISFLLGAVTSVVSGFLGMKIATYANARTTLEARKGVGKAFITAFRSGAVMG 198
              +A  +  +FLLGA+ S ++G++GM ++  AN R +  AR+   +A   A R+G    
Sbjct: 161 --MSAYITVAAFLLGALCSGIAGYVGMWVSVRANVRVSSAARRSAREALQIAVRAGGFSA 220

Query: 199 FLLAANGLLVLFIAINVFKLYYGEDWGGLFE------SITGYGLGGSSMALFGRVGGGIY 258
            ++    ++ + I  + F ++ G    G          + GYG G S +ALF ++GGGIY
Sbjct: 221 LVVVGMAVIGIAILYSTFYVWLGVGSPGSMNVTDLPLLLVGYGFGASFVALFAQLGGGIY 280

Query: 259 TKAADVGADLVGKVERNIPEDDPRNPAVIADNVGDNVGDIAGMGSDLFGSYAESSCAALV 318
           TK ADVGADLVGKVE+ IPEDDPRNPAVIAD VGDNVGD A  G+DLF S A    +A++
Sbjct: 281 TKGADVGADLVGKVEQGIPEDDPRNPAVIADLVGDNVGDCAARGADLFESIAAEIISAMI 340

Query: 319 VASISSFGNNHELTP--MLYPLIVSSVGILVCLITTLFATDFFEIKAVKEIEPALKKQLI 378
           +    +     E     +L+PL+V S  +++  I  L       IK  ++       +  
Sbjct: 341 LGGTMAKKCKIEDPSGFILFPLVVHSFDLIISSIGIL------SIKGTRDASVKSPVEDP 400

Query: 379 ISTVLMTFGIAIVTWVAVPSTFTIFNFGTQKVVQNWKLF-LCVAVGLWAGLIIGFVTEYY 438
           ++ +   + + I+  V      T +   T++    W  F LC  VG+    I  ++++YY
Sbjct: 401 MAVLQKGYSLTIILAVITFGASTRWLLYTEQAPSAWFNFALCGLVGIITAYIFVWISKYY 460

Query: 439 TSNAYSPVQDVADSCRTGAATNVIFGLALGYKSVIIPIFAIAVSIFVSFTF--------- 498
           T   + PV+ +A +  TG  TN+I G++LG +S  +P+  I+V+I  ++           
Sbjct: 461 TDYKHEPVRTLALASSTGHGTNIIAGVSLGLESTALPVLTISVAIISAYWLGNTSGLVDE 520

Query: 499 -----AAMYGIAVAALGMLSTIATGLAIDAYGPISDNAGGIAEMAGMSHRIRERTDALDA 558
                  ++G AVA +GMLST A  L +D +GPI+DNAGGI EM+     +RE TD LDA
Sbjct: 521 NGIPTGGLFGTAVATMGMLSTAAYVLTMDMFGPIADNAGGIVEMSQQPESVREITDLLDA 580

Query: 559 AGNTTAAIGKGFAIGSAALVSLALFGAFV------SRAGVTSVDVLTPKVFIGLIVGAML 618
            GNTT A  KGFAIGSAAL S  LF A++      +      VD+  P+VF+G ++GAML
Sbjct: 581 VGNTTKATTKGFAIGSAALASFLLFSAYMDEVSAFANVSFKEVDIAIPEVFVGGLLGAML 640

Query: 619 PYWFSAMTMKSVGSAALKMVEEVRRQFNTIPGLMEGTAKPDYATCVKISTDASIKEMIPP 678
            + FSA    +VG  A ++V EVRRQF   PG+ME   KPDY+ CV I   A+++EMI P
Sbjct: 641 IFLFSAWACAAVGRTAQEVVNEVRRQFIERPGIMEYKEKPDYSRCVAIVASAALREMIKP 700

Query: 679 GALVMLTPLIVGILF------------GVETLSGVLAGSLVSGVQIAISASNTGGAWDNA 738
           GAL + +P++VG++F            G + ++ +L  + V G+ +A+  +  GGAWDNA
Sbjct: 701 GALAIASPIVVGLVFRILGYYTGQPLLGAKVVASMLMFATVCGILMALFLNTAGGAWDNA 760

Query: 739 KKYIEAGASEHARTLGPKGSDPHKAAVIGDTIGDPLKDTSGPSLNILIKLMAVESLVFAP 759
           KKYIE GA      LG KGS+ HKAAV GDT+GDP KDT+GPS+++LIK++A  +LV AP
Sbjct: 761 KKYIETGA------LGGKGSEAHKAAVTGDTVGDPFKDTAGPSIHVLIKMLATITLVMAP 801

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P216160.0e+0092.27Pyrophosphate-energized vacuolar membrane proton pump OS=Vigna radiata var. radi... [more]
P314140.0e+0087.68Pyrophosphate-energized vacuolar membrane proton pump 1 OS=Arabidopsis thaliana ... [more]
Q065720.0e+0087.30Pyrophosphate-energized vacuolar membrane proton pump OS=Hordeum vulgare OX=4513... [more]
Q72Q293.5e-19956.61Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Q8F6413.5e-19956.61Putative K(+)-stimulated pyrophosphate-energized sodium pump OS=Leptospira inter... [more]
Match NameE-valueIdentityDescription
XP_023515840.10.0100.00pyrophosphate-energized vacuolar membrane proton pump [Cucurbita pepo subsp. pep... [more]
KAG7023485.10.099.61hypothetical protein SDJN02_14510, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022960721.10.099.48pyrophosphate-energized vacuolar membrane proton pump [Cucurbita moschata] >KAG6... [more]
XP_022987612.10.099.48pyrophosphate-energized vacuolar membrane proton pump [Cucurbita maxima][more]
XP_022929683.10.096.99pyrophosphate-energized vacuolar membrane proton pump-like [Cucurbita moschata] ... [more]
Match NameE-valueIdentityDescription
A0A6J1H8E20.099.48H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111461438 PE=3 ... [more]
A0A6J1JAU20.099.48H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111485119 PE=3 SV... [more]
A0A6J1ESX20.096.99H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=LOC111436191 PE=3 ... [more]
A0A6J1KSL00.096.73H(+)-exporting diphosphatase OS=Cucurbita maxima OX=3661 GN=LOC111496068 PE=3 SV... [more]
O826800.096.60H(+)-exporting diphosphatase OS=Cucurbita moschata OX=3662 GN=PHP-1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G15690.10.0e+0087.68Inorganic H pyrophosphatase family protein [more]
AT1G15690.21.6e-30386.46Inorganic H pyrophosphatase family protein [more]
AT1G16780.14.1e-11839.24Inorganic H pyrophosphatase family protein [more]
AT1G78920.15.9e-11738.50vacuolar H+-pyrophosphatase 2 [more]
AT1G78920.25.9e-11738.50vacuolar H+-pyrophosphatase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004131Pyrophosphate-energised proton pumpTIGRFAMTIGR01104TIGR01104coord: 12..764
e-value: 0.0
score: 1232.1
IPR004131Pyrophosphate-energised proton pumpPIRSFPIRSF001265H+-PPtasecoord: 11..767
e-value: 8.1E-283
score: 938.2
IPR004131Pyrophosphate-energised proton pumpPFAMPF03030H_PPasecoord: 24..754
e-value: 4.7E-257
score: 854.8
IPR004131Pyrophosphate-energised proton pumpPANTHERPTHR31998K(+)-INSENSITIVE PYROPHOSPHATE-ENERGIZED PROTON PUMPcoord: 4..767
IPR004131Pyrophosphate-energised proton pumpHAMAPMF_01129PPase_energized_pumpcoord: 10..759
score: 23.131685
NoneNo IPR availablePANTHERPTHR31998:SF38PYROPHOSPHATE-ENERGIZED VACUOLAR MEMBRANE PROTON PUMPcoord: 4..767

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g05210.1Cp4.1LG18g05210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0004427 inorganic diphosphatase activity
molecular_function GO:0009678 pyrophosphate hydrolysis-driven proton transmembrane transporter activity