Cp4.1LG18g04970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g04970
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmitogen-activated protein kinase kinase kinase 17-like
LocationCp4.1LG18: 5797740 .. 5798741 (-)
RNA-Seq ExpressionCp4.1LG18g04970
SyntenyCp4.1LG18g04970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAAGTCCTGTGTTGTCTCACAAGTTAACCAACAATGTCACAACATTGTTAACCTGCACGAGTTCTTGAAAACAAAGAGAGGCATCGTGAAGTGCGCAAAGAAGTCACAATCGAGCAACAATTGGAACAACAACGTGAAACACAACTACGGAAGATTTGGTAACTTCGCAATCGAAGAATATCACGGTTCATTCTGGATTGGTGATCGCCTCATTCGCAAAAGTGGATCTGGCCTTGTTTTCTTGGCCAAAAACAAATCATCCACCAACTCAAATCCAGAGTTTCTTGTCAAATCTTGTTTAATGGAGTCATCTTCTTCACTCCAATACGAGAAGGAAGTGCTACGCAATCTAGGGCCATGCTCTAATCTAGTGCGTTGTTATGGAGATGAAATAACAATGAACAAATCAGGCGAACAGATTTACAATGTCGTGTTCGAGTATTGCCGTGGCTCAAGCCTGCGTGATCACATTATCAAATTTGGACGTAATGGATTACCAGAACCCATAGTTCGTCAGTACACAAGAGACATTGTTCATGGACTCTGTTACATGCATTGCAATGCTGGTTACATTCACGGTGACATAAAACCAAGAAACATCTTACTATCACCAAGTGATCATCAAGGTGGTTGTTTGGTGGCTAAGTTAGCTAGTTTTGGGCTAGCTAGGAAGCTCACTCCTGAAGTGTTATGCGACGAGGAAATCAGTGGTTCAGGATCATACATAGCGCCAGAGTTGGCCAAAGATGGCTATCTAGACTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGCTAGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAAAGTGCTTATAATTATCTAATGGACTGCAACCAGGAGATACCAGAAATCCCAAGTAAGATATCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATCAGAAGTCCATACAAAAGAAGACCCACTTGGCTGTTAGTCAAACATCCTTTTGTTGCTGAATGA

mRNA sequence

ATGACGAAGTCCTGTGTTGTCTCACAAGTTAACCAACAATGTCACAACATTGTTAACCTGCACGAGTTCTTGAAAACAAAGAGAGGCATCGTGAAGTGCGCAAAGAAGTCACAATCGAGCAACAATTGGAACAACAACGTGAAACACAACTACGGAAGATTTGGTAACTTCGCAATCGAAGAATATCACGGTTCATTCTGGATTGGTGATCGCCTCATTCGCAAAAGTGGATCTGGCCTTGTTTTCTTGGCCAAAAACAAATCATCCACCAACTCAAATCCAGAGTTTCTTGTCAAATCTTGTTTAATGGAGTCATCTTCTTCACTCCAATACGAGAAGGAAGTGCTACGCAATCTAGGGCCATGCTCTAATCTAGTGCGTTGTTATGGAGATGAAATAACAATGAACAAATCAGGCGAACAGATTTACAATGTCGTGTTCGAGTATTGCCGTGGCTCAAGCCTGCGTGATCACATTATCAAATTTGGACGTAATGGATTACCAGAACCCATAGTTCGTCAGTACACAAGAGACATTGTTCATGGACTCTGTTACATGCATTGCAATGCTGGTTACATTCACGGTGACATAAAACCAAGAAACATCTTACTATCACCAAGTGATCATCAAGGTGGTTGTTTGGTGGCTAAGTTAGCTAGTTTTGGGCTAGCTAGGAAGCTCACTCCTGAAGTGTTATGCGACGAGGAAATCAGTGGTTCAGGATCATACATAGCGCCAGAGTTGGCCAAAGATGGCTATCTAGACTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGCTAGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAAAGTGCTTATAATTATCTAATGGACTGCAACCAGGAGATACCAGAAATCCCAAGTAAGATATCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATCAGAAGTCCATACAAAAGAAGACCCACTTGGCTGTTAGTCAAACATCCTTTTGTTGCTGAATGA

Coding sequence (CDS)

ATGACGAAGTCCTGTGTTGTCTCACAAGTTAACCAACAATGTCACAACATTGTTAACCTGCACGAGTTCTTGAAAACAAAGAGAGGCATCGTGAAGTGCGCAAAGAAGTCACAATCGAGCAACAATTGGAACAACAACGTGAAACACAACTACGGAAGATTTGGTAACTTCGCAATCGAAGAATATCACGGTTCATTCTGGATTGGTGATCGCCTCATTCGCAAAAGTGGATCTGGCCTTGTTTTCTTGGCCAAAAACAAATCATCCACCAACTCAAATCCAGAGTTTCTTGTCAAATCTTGTTTAATGGAGTCATCTTCTTCACTCCAATACGAGAAGGAAGTGCTACGCAATCTAGGGCCATGCTCTAATCTAGTGCGTTGTTATGGAGATGAAATAACAATGAACAAATCAGGCGAACAGATTTACAATGTCGTGTTCGAGTATTGCCGTGGCTCAAGCCTGCGTGATCACATTATCAAATTTGGACGTAATGGATTACCAGAACCCATAGTTCGTCAGTACACAAGAGACATTGTTCATGGACTCTGTTACATGCATTGCAATGCTGGTTACATTCACGGTGACATAAAACCAAGAAACATCTTACTATCACCAAGTGATCATCAAGGTGGTTGTTTGGTGGCTAAGTTAGCTAGTTTTGGGCTAGCTAGGAAGCTCACTCCTGAAGTGTTATGCGACGAGGAAATCAGTGGTTCAGGATCATACATAGCGCCAGAGTTGGCCAAAGATGGCTATCTAGACTGGCCTGCTGACATTTGGGCACTTGGATGCGTGGTGCTAGAGATGTTGACTGGAAAACCAGCATGGAGCTTTGAAAGTGCTTATAATTATCTAATGGACTGCAACCAGGAGATACCAGAAATCCCAAGTAAGATATCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATCAGAAGTCCATACAAAAGAAGACCCACTTGGCTGTTAGTCAAACATCCTTTTGTTGCTGAATGA

Protein sequence

MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIEEYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Homology
BLAST of Cp4.1LG18g04970 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 1.2e-42
Identity = 93/255 (36.47%), Postives = 147/255 (57.65%), Query Frame = 0

Query: 85  KNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYN 144
           K+++S +      VKS     ++SL  EK VL +LG C  ++RCYG++ T+ ++GE+++N
Sbjct: 22  KSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTV-ENGEEMHN 81

Query: 145 VVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILL 204
           ++ EY    SL  ++ K G  GLPE  VR++T  ++ GL ++H   G+ H DIK  NILL
Sbjct: 82  LLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAK-GFAHCDIKLANILL 141

Query: 205 SPSDHQGGCLVAKLASFGLARKLTPEVLC---DEEISGSGSYIAPELAKDGYLDWPADIW 264
               + G     K+A FGLA ++  ++       EI G+  Y+APE   D      AD+W
Sbjct: 142 F---NDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVW 201

Query: 265 ALGCVVLEMLTGKPAWSFESAYNYL-----MDCNQEIPEIPSKISSAGRDFVKRCLIRSP 324
           ALGC V+EM +GK AWS +   +++     +    E+P+IP  +S  G+DF+ +C ++ P
Sbjct: 202 ALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDP 261

Query: 325 YKRRPTWLLVKHPFV 332
            KR    +L+ H FV
Sbjct: 262 AKRWTAEMLLNHSFV 268

BLAST of Cp4.1LG18g04970 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.2e-32
Identity = 82/271 (30.26%), Postives = 142/271 (52.40%), Query Frame = 0

Query: 67  WIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLV 126
           W   R++ +  +  V+ A   +  NS+    VKS  +  S  LQ E ++L +L     ++
Sbjct: 3   WTRGRILGRGSTATVYAA---AGHNSDEILAVKSSEVHRSEFLQREAKILSSLS-SPYVI 62

Query: 127 RCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYM 186
              G E     +G  +YN++ EY    +L D   K G   + E  V +YTRDI+ GL Y+
Sbjct: 63  GYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDG-GRVDETRVVKYTRDILKGLEYI 122

Query: 187 HCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSYIAP 246
           H + G +H D+K  N+++S          AK+A FG A+++ P  + +  + G+ +++AP
Sbjct: 123 H-SKGIVHCDVKGSNVVISEKGE------AKIADFGCAKRVDP--VFESPVMGTPAFMAP 182

Query: 247 ELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYN------YLMDCNQEIPEIPSKI 306
           E+A+       +DIWA+GC ++EM+TG P W+   +        Y +  + E PE+P  +
Sbjct: 183 EVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLL 242

Query: 307 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 332
           +   +DF+++CL R   +R     L+ HPF+
Sbjct: 243 AEEAKDFLEKCLKREANERWTATQLLNHPFL 259

BLAST of Cp4.1LG18g04970 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 2.1e-31
Identity = 98/317 (30.91%), Postives = 158/317 (49.84%), Query Frame = 0

Query: 37  SQSSNNWNNNVKHNYGRFGNFAIEEY------HGSFWIGDRLIRKSGSGLVFLAKNKSS- 96
           + ++NN NNN    +  F +  I E+      H   W   +++ + G G V+L  NK + 
Sbjct: 134 NNNNNNNNNNNIIQHTDFPSLIINEHEELISNHNIKWQKGQILGRGGYGSVYLGLNKDTG 193

Query: 97  ------------TNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNK 156
                        NS+P+  +K+ ++    S   E EV+R+L    N+VR  G  +    
Sbjct: 194 ELFAVKQLEIVDINSDPK--LKNMIL----SFSKEIEVMRSLRH-DNIVRYLGTSL---- 253

Query: 157 SGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDI 216
             +   +V  EY  G S+   + KFG     E +++ YT+ I+ GL ++H N+  IH DI
Sbjct: 254 -DQSFLSVFLEYIPGGSISSLLGKFG--AFSENVIKVYTKQILQGLSFLHANS-IIHRDI 313

Query: 217 KPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWP 276
           K  NIL+   D +G   + KL+ FG ++  +  V   + + G+  ++APE+ K       
Sbjct: 314 KGANILI---DTKG---IVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRS 373

Query: 277 ADIWALGCVVLEMLTGKPAWS---FESAYNYLMDCNQEIPEIPSKISSAGRDFVKRCLIR 332
           +DIW+LGCV++EM T +P WS     +A  Y +  +  IP IPS +S    DF+  C  R
Sbjct: 374 SDIWSLGCVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKR 429

BLAST of Cp4.1LG18g04970 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.0e-30
Identity = 95/276 (34.42%), Postives = 138/276 (50.00%), Query Frame = 0

Query: 64  GSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSC-----LMESSSSLQYEKEVLRN 123
           GS W   RL+     G V+L  N  S        V  C       ES+  L  E  VL  
Sbjct: 397 GSRWKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSR 456

Query: 124 LGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRD 183
           L    N+V+ YG E   +K    +Y +  EY  G S+   + ++G+ G  E  +R YT+ 
Sbjct: 457 LRH-QNIVQYYGSETVDDK----LY-IYLEYVSGGSIYKLLQEYGQFG--ENAIRNYTQQ 516

Query: 184 IVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEIS 243
           I+ GL Y+H     +H DIK  NIL+ P          K+A FG+A+ +T +        
Sbjct: 517 ILSGLAYLHAK-NTVHRDIKGANILVDPHGR------VKVADFGMAKHITAQ-SGPLSFK 576

Query: 244 GSGSYIAPELAKDGY-LDWPADIWALGCVVLEMLTGKPAWS-FESA-YNYLMDCNQEIPE 303
           GS  ++APE+ K+    +   DIW+LGC VLEM T KP WS +E     + +  ++E+P+
Sbjct: 577 GSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKIGNSKELPD 636

Query: 304 IPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 332
           IP  +S  G+DFV++CL R+P  R     L+ H FV
Sbjct: 637 IPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of Cp4.1LG18g04970 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 2.0e-29
Identity = 84/238 (35.29%), Postives = 129/238 (54.20%), Query Frame = 0

Query: 99  KSCLMESSSSLQYEKEVLRNLGPC-SNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRD 158
           K CL +    L  E  +L  L  C  N+V+ YG E++     E+  +V  EY  G S+  
Sbjct: 255 KECLKQ----LNQEINLLNQL--CHPNIVQYYGSELS-----EETLSVYLEYVSGGSIHK 314

Query: 159 HIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAK 218
            +  +G     EP+++ YTR I+ GL Y+H     +H DIK  NIL+ P+         K
Sbjct: 315 LLKDYG--SFTEPVIQNYTRQILAGLAYLH-GRNTVHRDIKGANILVDPNGE------IK 374

Query: 219 LASFGLARKLTPEVLCDEEISGSGSYIAPE--LAKDGYLDWPADIWALGCVVLEMLTGKP 278
           LA FG+A+ +T          GS  ++APE  ++++GY     DIW+LGC +LEM T KP
Sbjct: 375 LADFGMAKHVT-AFSTMLSFKGSPYWMAPEVVMSQNGYTH-AVDIWSLGCTILEMATSKP 434

Query: 279 AWS-FES-AYNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 332
            WS FE  A  + +  +++ PEIP  +S+  ++F++ CL R+P  R     L++HPF+
Sbjct: 435 PWSQFEGVAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

BLAST of Cp4.1LG18g04970 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697 bits (1800), Expect = 7.60e-254
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60
           MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60

Query: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120
           EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120

Query: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180
           PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV
Sbjct: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180

Query: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240
           HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS
Sbjct: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240

Query: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300
           GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI
Sbjct: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300

Query: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

BLAST of Cp4.1LG18g04970 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678 bits (1749), Expect = 4.54e-246
Identity = 325/333 (97.60%), Postives = 328/333 (98.50%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60
           MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSS NWNNNVKHNYGRFGNFAIE
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120
           EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSN EFLVKSCLMESSSSLQYEKEVL NLG
Sbjct: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNLG 120

Query: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180
           PCSNLVRCYGDEITMNKSGEQIYNVVFEYC GSSLRDHIIKFGRNGLPEPIVR+YTRDIV
Sbjct: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIV 180

Query: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240
            GLCYMHCNAGYIHGDIKPRNILLSPSDH+GGCLVAKLASFGLARKLTPEVLCDEEISGS
Sbjct: 181 RGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEEISGS 240

Query: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300
           GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLM+CNQEIPEIPSKI
Sbjct: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEIPSKI 300

Query: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

BLAST of Cp4.1LG18g04970 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 671 bits (1732), Expect = 1.77e-243
Identity = 322/333 (96.70%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60
           MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSS NWNNNVKHNYGRFGNFAIE
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120
           E HGSFWIGDRLIR+SGSGLVFLAKNKSSTNSN EFLVKSCLMESSSSLQYEKEVL NLG
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNLG 120

Query: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180
           PCSNLVRCYGDEITM+KSGEQIYNVVFEYC GSSLRDHIIKFGRNGLPEPIVR+YTRDIV
Sbjct: 121 PCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIV 180

Query: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240
            GLCYMHCNAGYIHGDIKPRNILLSPSDH+GGCLVAKLASFGLARKLTPEVLCDEEISGS
Sbjct: 181 RGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEEISGS 240

Query: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300
           GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLM+CNQEIPEIPSKI
Sbjct: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEIPSKI 300

Query: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

BLAST of Cp4.1LG18g04970 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 670 bits (1728), Expect = 7.22e-243
Identity = 322/333 (96.70%), Postives = 326/333 (97.90%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60
           MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSS NWNNNVKHNYGRFGNFAIE
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120
           E HGSFWIGDRLIRKSGSGLVFLAKNKSSTNSN EFLVKSCLMESSSSLQYEKEVL NLG
Sbjct: 61  ECHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNLG 120

Query: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180
           PCSNLVRCYGDEITMNKSGEQIYNVVFEYC GSSLRDHIIKFGRNGLPEPIVR+YTRDIV
Sbjct: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIV 180

Query: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240
            GLCY+HCNAGYIHGDIKPRNILLSPSD +GGCLVAKLASFGLARKLTPEVLCDEEISGS
Sbjct: 181 RGLCYIHCNAGYIHGDIKPRNILLSPSDRKGGCLVAKLASFGLARKLTPEVLCDEEISGS 240

Query: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300
           GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLM+CNQEIPEIPSKI
Sbjct: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEIPSKI 300

Query: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

BLAST of Cp4.1LG18g04970 vs. NCBI nr
Match: XP_022987487.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita maxima])

HSP 1 Score: 476 bits (1226), Expect = 5.25e-168
Identity = 226/231 (97.84%), Postives = 228/231 (98.70%), Query Frame = 0

Query: 103 MESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKF 162
           MESSSSLQYEKEVL NLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYC GSSLRDHIIKF
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 163 GRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFG 222
           GRNGLPEPIVR+YTRDIVHGLCYMHCNA YIHGDIKPRNILLSPSDH+GGCLVAKLASFG
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFG 120

Query: 223 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 282
           LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA
Sbjct: 121 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 180

Query: 283 YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 181 YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 231

BLAST of Cp4.1LG18g04970 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 671 bits (1732), Expect = 8.58e-244
Identity = 322/333 (96.70%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIE 60
           MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSS NWNNNVKHNYGRFGNFAIE
Sbjct: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIE 60

Query: 61  EYHGSFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG 120
           E HGSFWIGDRLIR+SGSGLVFLAKNKSSTNSN EFLVKSCLMESSSSLQYEKEVL NLG
Sbjct: 61  ECHGSFWIGDRLIRRSGSGLVFLAKNKSSTNSNSEFLVKSCLMESSSSLQYEKEVLCNLG 120

Query: 121 PCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIV 180
           PCSNLVRCYGDEITM+KSGEQIYNVVFEYC GSSLRDHIIKFGRNGLPEPIVR+YTRDIV
Sbjct: 121 PCSNLVRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIV 180

Query: 181 HGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGS 240
            GLCYMHCNAGYIHGDIKPRNILLSPSDH+GGCLVAKLASFGLARKLTPEVLCDEEISGS
Sbjct: 181 RGLCYMHCNAGYIHGDIKPRNILLSPSDHKGGCLVAKLASFGLARKLTPEVLCDEEISGS 240

Query: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQEIPEIPSKI 300
           GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLM+CNQEIPEIPSKI
Sbjct: 241 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNCNQEIPEIPSKI 300

Query: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 301 SSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333

BLAST of Cp4.1LG18g04970 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 476 bits (1226), Expect = 2.54e-168
Identity = 226/231 (97.84%), Postives = 228/231 (98.70%), Query Frame = 0

Query: 103 MESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKF 162
           MESSSSLQYEKEVL NLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYC GSSLRDHIIKF
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 163 GRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFG 222
           GRNGLPEPIVR+YTRDIVHGLCYMHCNA YIHGDIKPRNILLSPSDH+GGCLVAKLASFG
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKGGCLVAKLASFG 120

Query: 223 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 282
           LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA
Sbjct: 121 LARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESA 180

Query: 283 YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE
Sbjct: 181 YNYLMDCNQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 231

BLAST of Cp4.1LG18g04970 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 3.38e-162
Identity = 238/341 (69.79%), Postives = 268/341 (78.59%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFG-NFAI 60
           MTKSCVVS+V Q C   VNLHEFLKTKR I+K AKKS  +NNWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSRV-QFC---VNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSFWIGDRLIRKSGSGLVFLAK------NKSSTNSNPEFLVKSCLMESSSSLQYEK 120
           EE+HGS W G  L RK+ S L+FLAK      N S++N   EFLVKS L ESSSSL +EK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVR 180
           EVL NLGP  NLV CYGDEIT++KSGE++YNV FEYC GSSLRDHI KFG NGL E  VR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 QYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLC 240
           +YTRDIVHGL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240

Query: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQE- 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSF  A+ YLMDCN E 
Sbjct: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEK 300

Query: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           IPEIP  IS  GRDF+K+CLIRSPYKRRP+WLL+KHPFV +
Sbjct: 301 IPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVCQ 330

BLAST of Cp4.1LG18g04970 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 3.38e-162
Identity = 238/341 (69.79%), Postives = 268/341 (78.59%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFG-NFAI 60
           MTKSCVVS+V Q C   VNLHEFLKTKR I+K AKKS  +NNWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSRV-QFC---VNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSFWIGDRLIRKSGSGLVFLAK------NKSSTNSNPEFLVKSCLMESSSSLQYEK 120
           EE+HGS W G  L RK+ S L+FLAK      N S++N   EFLVKS L ESSSSL +EK
Sbjct: 61  EEFHGSIWSGVHLFRKTESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEK 120

Query: 121 EVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVR 180
           EVL NLGP  NLV CYGDEIT++KSGE++YNV FEYC GSSLRDHI KFG NGL E  VR
Sbjct: 121 EVLSNLGPFMNLVDCYGDEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVR 180

Query: 181 QYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLC 240
           +YTRDIVHGL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 KYTRDIVHGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240

Query: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQE- 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSF  A+ YLMDCN E 
Sbjct: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDCNNEK 300

Query: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           IPEIP  IS  GRDF+K+CLIRSPYKRRP+WLL+KHPFV +
Sbjct: 301 IPEIPKNISREGRDFIKKCLIRSPYKRRPSWLLIKHPFVCQ 330

BLAST of Cp4.1LG18g04970 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 462 bits (1190), Expect = 2.77e-161
Identity = 236/341 (69.21%), Postives = 265/341 (77.71%), Query Frame = 0

Query: 1   MTKSCVVSQVNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFG-NFAI 60
           MTKSCVVSQV        NLHEFLKTKR I+K AKKS   NNWN+ +KHNY +FG +F I
Sbjct: 1   MTKSCVVSQVQISG----NLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTI 60

Query: 61  EEYHGSFWIGDRLIRKSGSGLVFLAK------NKSSTNSNPEFLVKSCLMESSSSLQYEK 120
           EE+HGS W G  L RK+ S LVFLAK      N S++N   EFLVKS LMESSSSL++EK
Sbjct: 61  EEFHGSIWTGSLLFRKTESTLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEK 120

Query: 121 EVLRNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVR 180
           EVL NLGP +NLV CYGDEIT+ KSGE++YNV FEYC GSSLR+HI+KFG NGL +  VR
Sbjct: 121 EVLSNLGPFTNLVDCYGDEITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVR 180

Query: 181 QYTRDIVHGLCYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLC 240
           +YTRDIV GL YMHCN  YIHGDIK RNILLS         +AKLASFGLARKLT EV+C
Sbjct: 181 RYTRDIVRGLYYMHCNGRYIHGDIKSRNILLSHG-------MAKLASFGLARKLTAEVIC 240

Query: 241 DEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDCNQE- 300
           +EEISGSG Y +PELA++GYL WPADIWALGCVVLEM TGK AWSFE AY YLMD N E 
Sbjct: 241 EEEISGSGPYASPELAREGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDSNNEK 300

Query: 301 IPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 333
           IPEIP  IS  GRDF+K+CLIRSPYKRRP WLL+KHPFV +
Sbjct: 301 IPEIPKNISREGRDFIKKCLIRSPYKRRPIWLLIKHPFVCQ 330

BLAST of Cp4.1LG18g04970 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 186.0 bits (471), Expect = 4.8e-47
Identity = 111/275 (40.36%), Postives = 159/275 (57.82%), Query Frame = 0

Query: 67  WIGDRLIRKSGSGLVFLAKNK-SSTNSNPEFL-VKSCLMESSSSLQYEKEVLRNLGPCSN 126
           WI    I       V LA    SS+ + P  + VKS  +  S++L+ E++VL +LG CS 
Sbjct: 3   WIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSE 62

Query: 127 LVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLC 186
           +VRC+G+  T+ ++GE+IYN+  EY  G SL D  IK     LPE  VR++TR IV GLC
Sbjct: 63  IVRCFGEGRTV-ENGEEIYNLFLEYASGGSLADR-IKSSGEALPEFEVRRFTRSIVKGLC 122

Query: 187 YMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSYI 246
           ++H N G+ H DIK  N+L+       G    K++ FGLA++ + EV    EI G+  Y+
Sbjct: 123 HIHGN-GFTHCDIKLENVLVF------GDGDVKISDFGLAKRRSGEVCV--EIRGTPLYM 182

Query: 247 APELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFE-SAYNYLMD------CNQEIPEIP 306
           APE    G  + PADIWALGC V+EM +GK AW  E    N +M          E+P IP
Sbjct: 183 APESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIP 242

Query: 307 SKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFVA 333
            ++S  G+DFV +C +++  +R    +L+ HPF+A
Sbjct: 243 VELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLA 266

BLAST of Cp4.1LG18g04970 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 178.7 bits (452), Expect = 7.7e-45
Identity = 101/275 (36.73%), Postives = 150/275 (54.55%), Query Frame = 0

Query: 67  WIGDRLIRKSGSGLVFLA--KNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLG-PCS 126
           WI    I       V LA   N  S    P   VKS     ++SL  EK VL NLG  C+
Sbjct: 3   WIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCN 62

Query: 127 NLVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGL 186
            +VRC+G++ T+ ++GE+++N+  EY    SL  ++ K    G+PE  VR++T  ++ GL
Sbjct: 63  EIVRCFGEDRTV-ENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGL 122

Query: 187 CYMHCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCD--EEISGSG 246
            ++H N G+ H D+K  NILL       G    K+A FGLA+++      +   +I G+ 
Sbjct: 123 RHIHAN-GFAHCDLKLGNILLF------GDGAVKIADFGLAKRIGDLTALNYGVQIRGTP 182

Query: 247 SYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYL-----MDCNQEIPEI 306
            Y+APE   D       D+WALGCVV+EM +GK AWS +   N++     +    E+P I
Sbjct: 183 LYMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMI 242

Query: 307 PSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 332
           P ++S  GRDF+ +C ++ P KR    +L+ HPFV
Sbjct: 243 PEELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFV 269

BLAST of Cp4.1LG18g04970 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 175.3 bits (443), Expect = 8.5e-44
Identity = 93/255 (36.47%), Postives = 147/255 (57.65%), Query Frame = 0

Query: 85  KNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLVRCYGDEITMNKSGEQIYN 144
           K+++S +      VKS     ++SL  EK VL +LG C  ++RCYG++ T+ ++GE+++N
Sbjct: 22  KSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGEDSTV-ENGEEMHN 81

Query: 145 VVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYMHCNAGYIHGDIKPRNILL 204
           ++ EY    SL  ++ K G  GLPE  VR++T  ++ GL ++H   G+ H DIK  NILL
Sbjct: 82  LLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAK-GFAHCDIKLANILL 141

Query: 205 SPSDHQGGCLVAKLASFGLARKLTPEVLC---DEEISGSGSYIAPELAKDGYLDWPADIW 264
               + G     K+A FGLA ++  ++       EI G+  Y+APE   D      AD+W
Sbjct: 142 F---NDGS---VKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPECVNDNEYGSAADVW 201

Query: 265 ALGCVVLEMLTGKPAWSFESAYNYL-----MDCNQEIPEIPSKISSAGRDFVKRCLIRSP 324
           ALGC V+EM +GK AWS +   +++     +    E+P+IP  +S  G+DF+ +C ++ P
Sbjct: 202 ALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEEGKDFLSKCFVKDP 261

Query: 325 YKRRPTWLLVKHPFV 332
            KR    +L+ H FV
Sbjct: 262 AKRWTAEMLLNHSFV 268

BLAST of Cp4.1LG18g04970 vs. TAIR 10
Match: AT3G45670.1 (Protein kinase superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.0e-41
Identity = 102/276 (36.96%), Postives = 154/276 (55.80%), Query Frame = 0

Query: 65  SFWIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSN 124
           S W+  RL+ +   G V+LA +K       E  +KS  +  + SL +E  +LR+L     
Sbjct: 95  SSWVKSRLLGEGAYGCVYLATSKDDIYKT-ERAIKSADVLKAWSLMHEGRILRSL-QSPF 154

Query: 125 LVRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLC 184
           ++RCYG EI    +G Q YN++ EYC G  L D +I+  + G+PE  V+Q+  D++ GL 
Sbjct: 155 VIRCYGHEIAREGTGHQ-YNLILEYCSGQCLAD-MIEDNQGGIPEFDVKQFAIDVLSGLS 214

Query: 185 YMHCNAGYIHGDIKPRNILLSPSDH--QGGCLVAKLASFGLARKLTPEVLCD--EEISGS 244
           Y+H     IH +IKP N+LLSP DH  +    + K+A FGL+ +   +   +    + G+
Sbjct: 215 YIH-RRNIIHCEIKPDNLLLSPVDHRFRSNGFLTKIADFGLSMEKGSKEYGNGRGHMRGT 274

Query: 245 GSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWS--FESAYNYLMDC---NQEIPE 304
             Y+APEL   G LD+  DI A GC VLEMLTGK  W    + A++  +D    +   P+
Sbjct: 275 TRYMAPELIGGGLLDFAVDICAFGCSVLEMLTGKRVWGEYGDLAHDDWVDLIGHSDLTPQ 334

Query: 305 IPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 332
           I  ++S+  +DF+ RCL++ P  R     LV HPF+
Sbjct: 335 ISIRLSAEAQDFLMRCLVKEPGSRWTIGELVDHPFL 365

BLAST of Cp4.1LG18g04970 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 160.2 bits (404), Expect = 2.8e-39
Identity = 96/270 (35.56%), Postives = 149/270 (55.19%), Query Frame = 0

Query: 67  WIGDRLIRKSGSGLVFLAKNKSSTNSNPEFLVKSCLMESSSSLQYEKEVLRNLGPCSNLV 126
           WI   +I +  +  V L      TNS   F VKS    SS+ LQ E+ +L  L     +V
Sbjct: 6   WIRGPIIGRGSTATVSL----GITNSGDFFAVKSAEFSSSAFLQREQSILSKLS-SPYIV 65

Query: 127 RCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCYM 186
           +  G  +T  ++ + +YN++ EY  G SL D +IK     LPEP++R YTR I+ GL Y+
Sbjct: 66  KYIGSNVT-KENDKLMYNLLMEYVSGGSLHD-LIKNSGGKLPEPLIRSYTRQILKGLMYL 125

Query: 187 HCNAGYIHGDIKPRNILLSPSDHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSYIAP 246
           H + G +H D+K +N+++       G  +AK+   G A+  T E   + E SG+ ++++P
Sbjct: 126 H-DQGIVHCDVKSQNVMI-------GGEIAKIVDLGCAK--TVEENENLEFSGTPAFMSP 185

Query: 247 ELAKDGYLDWPADIWALGCVVLEMLTGKPAW---SFESAYNYLMDCNQEIPEIPSKISSA 306
           E+A+     +PAD+WALGC V+EM TG   W   +   A  Y +    E P IP  +S  
Sbjct: 186 EVARGEEQSFPADVWALGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEK 245

Query: 307 GRDFVKRCLIRSPYKRRPTWLLVKHPFVAE 334
           G+DF+++CL + P +R     L++HPF+ E
Sbjct: 246 GQDFLRKCLRKDPKQRWTVEELLQHPFLDE 258

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SND61.2e-4236.47Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
O808884.2e-3230.26Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q54R822.1e-3130.91Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=... [more]
Q9CAD53.0e-3034.42Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3... [more]
F4HRJ42.0e-2935.29Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
XP_023516554.17.60e-254100.00mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe... [more]
KAG7023508.14.54e-24697.60Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
XP_022960664.11.77e-24396.70mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata][more]
KAG6589837.17.22e-24396.70Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper... [more]
XP_022987487.15.25e-16897.84mitogen-activated protein kinase kinase kinase 17-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1HBP58.58e-24496.70mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1JAH82.54e-16897.84mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
A0A5D3D3K93.38e-16269.79Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma... [more]
A0A1S3B8293.38e-16269.79mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656... [more]
A0A0A0LHV12.77e-16169.21Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829... [more]
Match NameE-valueIdentityDescription
AT4G36950.14.8e-4740.36mitogen-activated protein kinase kinase kinase 21 [more]
AT5G67080.17.7e-4536.73mitogen-activated protein kinase kinase kinase 19 [more]
AT3G50310.18.5e-4436.47mitogen-activated protein kinase kinase kinase 20 [more]
AT3G45670.11.0e-4136.96Protein kinase superfamily protein [more]
AT5G55090.12.8e-3935.56mitogen-activated protein kinase kinase kinase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 67..331
e-value: 3.6E-46
score: 169.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 93..331
e-value: 3.1E-38
score: 131.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 67..331
score: 34.845268
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 63..333
e-value: 1.3E-50
score: 174.4
NoneNo IPR availablePANTHERPTHR48011:SF56MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 3-LIKEcoord: 64..332
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 64..332
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 192..204
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 67..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g04970.1Cp4.1LG18g04970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity