Cp4.1LG18g04340 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG18g04340 vs. ExPASy Swiss-Prot
Match: J9U5U9 (Probable strigolactone esterase DAD2 OS=Petunia hybrida OX=4102 GN=DAD2 PE=1 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 3.1e-57
Identity = 108/261 (41.38%), Postives = 168/261 (64.37%), Query Frame = 0

Query: 10  LSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPNLY 69
           L  ALN +V+GSG   +VLAHGFGT+QS W+ ++    + YRVV++D    GS+ +P+ +
Sbjct: 5   LLDALNVRVVGSGERVLVLAHGFGTDQSAWNRILPFFLRDYRVVLYDLVCAGSV-NPDFF 64

Query: 70  DPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCSSP 129
           D ++Y++   + +DL+ +LD LG+    ++GHS+S +IG +AS +RP+LF +LIL+ +SP
Sbjct: 65  DFRRYTTLDPYVDDLLHILDALGIDCCAYVGHSVSAMIGILASIRRPELFSKLILIGASP 124

Query: 130 RYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRLRKCLHQMRP 189
           R++N EDY GGF+Q +IE++ + ME+NYE W   F+ L V    P ++    + L  MRP
Sbjct: 125 RFLNDEDYHGGFEQGEIEKVFSAMEANYEAWVNGFAPLAVGADVPAAVREFSRTLFNMRP 184

Query: 190 EVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRVINT 249
           ++ + +++TVF  D R +L  V +PC I  T  D + PASVA +++  + G  TV  +N 
Sbjct: 185 DITLFVSRTVFNSDMRGVLGLVKVPCHIFQTARDHSVPASVATYLKNHLGGKNTVHWLNI 244

Query: 250 NGHFPHLTAHLQLLQVLGEIL 271
            GH PHL+A   L Q L   L
Sbjct: 245 EGHLPHLSAPTLLAQELRRAL 264

BLAST of Cp4.1LG18g04340 vs. ExPASy Swiss-Prot
Match: Q10QA5 (Strigolactone esterase D14 OS=Oryza sativa subsp. japonica OX=39947 GN=D14 PE=1 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 2.0e-56
Identity = 104/257 (40.47%), Postives = 167/257 (64.98%), Query Frame = 0

Query: 14  LNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPNLYDPQK 73
           LN +V+GSG   +VL+HGFGT+QS W  ++  L++ +RVV++D    GS+ +P+ +D ++
Sbjct: 60  LNVRVVGSGERVVVLSHGFGTDQSAWSRVLPYLTRDHRVVLYDLVCAGSV-NPDHFDFRR 119

Query: 74  YSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCSSPRYIN 133
           Y +  A+ +DL+A+LD L +    F+GHS+S +IG +AS +RPDLF +L+L+ +SPR++N
Sbjct: 120 YDNLDAYVDDLLAILDALRIPRCAFVGHSVSAMIGILASIRRPDLFAKLVLIGASPRFLN 179

Query: 134 TEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRLRKCLHQMRPEVAV 193
             DY GGF+  +I+Q+   M +NY  WAT ++ L V    P ++    + L  MRP++++
Sbjct: 180 DSDYHGGFELEEIQQVFDAMGANYSAWATGYAPLAVGADVPAAVQEFSRTLFNMRPDISL 239

Query: 194 SLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRVINTNGHF 253
            + +TVF+ D R +L  V  PC ++ T  D++ PASVA +++  + G  TV  + T GH 
Sbjct: 240 HVCQTVFKTDLRGVLGMVRAPCVVVQTTRDVSVPASVAAYLKAHLGGRTTVEFLQTEGHL 299

Query: 254 PHLTAHLQLLQVLGEIL 271
           PHL+A   L QVL   L
Sbjct: 300 PHLSAPSLLAQVLRRAL 315

BLAST of Cp4.1LG18g04340 vs. ExPASy Swiss-Prot
Match: Q9SQR3 (Strigolactone esterase D14 OS=Arabidopsis thaliana OX=3702 GN=D14 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.2e-55
Identity = 103/258 (39.92%), Postives = 167/258 (64.73%), Query Frame = 0

Query: 13  ALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPNLYDPQ 72
           ALN +V+G+G   + LAHGFGT+QS W  ++   +Q YRVV++D    GS+ +P+ +D  
Sbjct: 9   ALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-NPDYFDFN 68

Query: 73  KYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCSSPRYI 132
           +Y++   + +DL+ ++D LG+ +  ++GHS+S +IG IAS +RP+LF +LIL+  SPR++
Sbjct: 69  RYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPRFL 128

Query: 133 NTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRLRKCLHQMRPEVA 192
           N EDY GGF++ +IE++ + ME+NYE W   F+ L V    P ++    + L  MRP+++
Sbjct: 129 NDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPDIS 188

Query: 193 VSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRVINTNGH 252
           + +++TVF  D R +L  V +P  ++ T  D++ PASVA +++  + G  TV  + T GH
Sbjct: 189 LFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTEGH 248

Query: 253 FPHLTAHLQLLQVLGEIL 271
            P L+A  QL Q L   L
Sbjct: 249 LPQLSAPAQLAQFLRRAL 265

BLAST of Cp4.1LG18g04340 vs. ExPASy Swiss-Prot
Match: A0A109QYD3 (Strigolactone esterase RMS3 OS=Pisum sativum OX=3888 GN=RMS3 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.9e-55
Identity = 102/251 (40.64%), Postives = 169/251 (67.33%), Query Frame = 0

Query: 9   PLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPNL 68
           P+  A N +V GSG + +V AHGFGT+QS W  ++   ++ Y+V+++D    GS+ +P+ 
Sbjct: 4   PILDAFNVRVEGSGDKYLVFAHGFGTDQSAWQRVLPYFTRSYKVILYDLVCAGSV-NPDH 63

Query: 69  YDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCSS 128
           +D ++Y++  A+ +DL+ +LD L +T   ++GHS+S + G +AS +RP+LF +LIL+ +S
Sbjct: 64  FDFRRYTTLDAYVDDLLNILDSLHVTRCAYVGHSISAMTGMLASIRRPELFSKLILIGAS 123

Query: 129 PRYINT-EDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRLRKCLHQM 188
           PR++N  E+Y GGF+Q +IE + + ME+NYE W   F+ L V    P ++    + L  M
Sbjct: 124 PRFLNDGENYHGGFEQGEIEHVFSAMEANYEAWVNGFAPLAVGADVPTAVREFSRTLFNM 183

Query: 189 RPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRVI 248
           RP++++ +++TVF  D R IL  V++PC I+ T  D++ PASVA +M++ I G  TV+ +
Sbjct: 184 RPDISLFVSRTVFNSDLRGILGLVNVPCCIMQTARDMSVPASVATYMKEHIGGKSTVQWL 243

Query: 249 NTNGHFPHLTA 259
           +T GH PHL+A
Sbjct: 244 DTEGHLPHLSA 253

BLAST of Cp4.1LG18g04340 vs. ExPASy Swiss-Prot
Match: Q9SZU7 (Probable esterase KAI2 OS=Arabidopsis thaliana OX=3702 GN=KAI2 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 9.4e-54
Identity = 101/260 (38.85%), Postives = 166/260 (63.85%), Query Frame = 0

Query: 8   GPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPN 67
           G +  A N KV+GSG   +VL HGFGT+QS+W ++V  L   YRVV++D        +P+
Sbjct: 2   GVVEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYD-NMGAGTTNPD 61

Query: 68  LYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCS 127
            +D  +YS+   ++ DLIA+L++L + S IF+GHS+S +IG +AS  RPDLF +++++ +
Sbjct: 62  YFDFDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISA 121

Query: 128 SPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVV-DEADPLSLSRLRKCLHQ 187
           SPRY+N  DY+GGF+Q D+ Q+   + SNY+ W   F+ L V  + D +++    + L  
Sbjct: 122 SPRYVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFN 181

Query: 188 MRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRV 247
           MRP++A+S+ +T+F+ D R+IL  V +PC IL +  D+A P  V+ ++   +     V V
Sbjct: 182 MRPDIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEV 241

Query: 248 INTNGHFPHLTAHLQLLQVL 267
           I ++GH P L++   ++ V+
Sbjct: 242 IPSDGHLPQLSSPDSVIPVI 260

BLAST of Cp4.1LG18g04340 vs. NCBI nr
Match: XP_023515491.1 (probable strigolactone esterase DAD2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545 bits (1405), Expect = 1.10e-195
Identity = 272/272 (100.00%), Postives = 272/272 (100.00%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG
Sbjct: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. NCBI nr
Match: XP_022987831.1 (probable strigolactone esterase DAD2 [Cucurbita maxima])

HSP 1 Score: 536 bits (1380), Expect = 7.11e-192
Identity = 266/272 (97.79%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSY AFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYSAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRY+NTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSL RL
Sbjct: 121 RLILLCSSPRYLNTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLIRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLH+MRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTK D+AAPASVAIFMQKQIKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. NCBI nr
Match: KAG6589894.1 (putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023565.1 putative strigolactone esterase DAD2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 534 bits (1376), Expect = 2.89e-191
Identity = 266/272 (97.79%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLS ALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSL+RL
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLNRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLHQMRPEV+VSLAKTVF VDEREILEKVDIPC ILHTK DIAAPASVAIFMQKQIKG
Sbjct: 181 RKCLHQMRPEVSVSLAKTVFGVDEREILEKVDIPCIILHTKTDIAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. NCBI nr
Match: XP_022961342.1 (probable strigolactone esterase DAD2 [Cucurbita moschata])

HSP 1 Score: 533 bits (1374), Expect = 5.84e-191
Identity = 265/272 (97.43%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLS ALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRYINTEDYEGGFDQSDIEQMIANME+NYENWATQFSSLVVDEADPLSLSRL
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMEANYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLH+MRPEVAVSLAKTVF VDER ILEKVDIPCTILHTK D+AAPASVAIFMQKQIKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFGVDERGILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. NCBI nr
Match: XP_022144907.1 (probable strigolactone esterase DAD2 [Momordica charantia])

HSP 1 Score: 446 bits (1148), Expect = 1.61e-156
Identity = 217/272 (79.78%), Postives = 244/272 (89.71%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLC+SPRYINTEDYEGGFD SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           +KCLH+MRPEVAVSLAKTVF  DER++LEKVDIPCTI+ TK DI  PASVA +MQK+IKG
Sbjct: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of Cp4.1LG18g04340 vs. ExPASy TrEMBL
Match: A0A6J1JFG1 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111485258 PE=4 SV=1)

HSP 1 Score: 536 bits (1380), Expect = 3.44e-192
Identity = 266/272 (97.79%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSY AFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYSAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRY+NTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSL RL
Sbjct: 121 RLILLCSSPRYLNTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLIRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLH+MRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTK D+AAPASVAIFMQKQIKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. ExPASy TrEMBL
Match: A0A6J1H9Y6 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111461858 PE=4 SV=1)

HSP 1 Score: 533 bits (1374), Expect = 2.83e-191
Identity = 265/272 (97.43%), Postives = 268/272 (98.53%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVMVENGPLS ALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW
Sbjct: 1   MVVMVENGPLSMALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ
Sbjct: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLCSSPRYINTEDYEGGFDQSDIEQMIANME+NYENWATQFSSLVVDEADPLSLSRL
Sbjct: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMEANYENWATQFSSLVVDEADPLSLSRL 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           RKCLH+MRPEVAVSLAKTVF VDER ILEKVDIPCTILHTK D+AAPASVAIFMQKQIKG
Sbjct: 181 RKCLHEMRPEVAVSLAKTVFGVDERGILEKVDIPCTILHTKTDVAAPASVAIFMQKQIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF
Sbjct: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272

BLAST of Cp4.1LG18g04340 vs. ExPASy TrEMBL
Match: A0A6J1CV13 (probable strigolactone esterase DAD2 OS=Momordica charantia OX=3673 GN=LOC111014472 PE=4 SV=1)

HSP 1 Score: 446 bits (1148), Expect = 7.80e-157
Identity = 217/272 (79.78%), Postives = 244/272 (89.71%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVM+ENG LS ALNA++MGSG EAMVLAHGFGTNQSLWD ++ KL Q YRVVVFDW+F 
Sbjct: 1   MVVMIENGLLSRALNARIMGSGAEAMVLAHGFGTNQSLWDKILPKLCQHYRVVVFDWSFS 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           G+IKDPNL+DPQKY SYY FA+DLIALL+ELGL+STIF+GHSMSGLIGCIA T+ P LFQ
Sbjct: 61  GAIKDPNLFDPQKYCSYYGFAQDLIALLEELGLSSTIFIGHSMSGLIGCIAYTQSPHLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RLILLC+SPRYINTEDYEGGFD SDIEQ+IAN+ESNYENWAT FSSLVVDE+DP+SL R 
Sbjct: 121 RLILLCASPRYINTEDYEGGFDNSDIEQIIANIESNYENWATNFSSLVVDESDPVSLGRF 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           +KCLH+MRPEVAVSLAKTVF  DER++LEKVDIPCTI+ TK DI  PASVA +MQK+IKG
Sbjct: 181 QKCLHEMRPEVAVSLAKTVFCSDERDVLEKVDIPCTIVQTKTDIVVPASVATYMQKKIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
           S TV VI+T GHFPHLTAHLQLLQVLG +LGF
Sbjct: 241 SSTVEVIDTQGHFPHLTAHLQLLQVLGAVLGF 272

BLAST of Cp4.1LG18g04340 vs. ExPASy TrEMBL
Match: A0A6J1KTG9 (probable strigolactone esterase DAD2 OS=Cucurbita maxima OX=3661 GN=LOC111496258 PE=4 SV=1)

HSP 1 Score: 434 bits (1117), Expect = 3.96e-152
Identity = 211/272 (77.57%), Postives = 242/272 (88.97%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVM++ G  STA+NAK++GSG E MVLAHGFG NQSLWD ++ KL+  YRV++FDWTF 
Sbjct: 1   MVVMLQTG-FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDWTFA 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNL+DP KYSSY  FAEDLI +LDELGLTSTIFLGHSMSGLIGC+A T RPDLFQ
Sbjct: 61  GSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTIRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RL+LLCSSPRYINTE YEGGF+QSDIEQ+IA++ESNY+NWAT F SLVVDEADP+S+SR 
Sbjct: 121 RLVLLCSSPRYINTEGYEGGFEQSDIEQIIASIESNYQNWATHFPSLVVDEADPVSVSRF 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           +KCLH+MRPEVA+ LAKTVF VDER+ILEKVDIPCTI+ TK DI  PASVA+FMQ +IKG
Sbjct: 181 QKCLHEMRPEVALPLAKTVFGVDERDILEKVDIPCTIVQTKTDIVVPASVAVFMQNKIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
            CTV+VINT+GHFPHLTAHL+LLQVLG ILGF
Sbjct: 241 CCTVQVINTDGHFPHLTAHLELLQVLGTILGF 271

BLAST of Cp4.1LG18g04340 vs. ExPASy TrEMBL
Match: A0A6J1F5V4 (probable strigolactone esterase DAD2 OS=Cucurbita moschata OX=3662 GN=LOC111441110 PE=4 SV=1)

HSP 1 Score: 432 bits (1111), Expect = 3.25e-151
Identity = 208/272 (76.47%), Postives = 242/272 (88.97%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVVM++ G  STA+NAK++GSG E MVLAHGFG NQSLWD ++ KL+  YRV++FDWTF 
Sbjct: 1   MVVMLQTG-FSTAVNAKIIGSGSEVMVLAHGFGGNQSLWDKILPKLTHHYRVLLFDWTFA 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           GSIKDPNL+DP KYSSY  FAEDLI +LDELGLTSTIFLGHSMSGLIGC+A TKRPDLFQ
Sbjct: 61  GSIKDPNLFDPHKYSSYSGFAEDLIKILDELGLTSTIFLGHSMSGLIGCVAYTKRPDLFQ 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
           RL+LLCSSPRYINTE YEGGF++SDI+Q+IA++ESNY+NWAT F SLVVDEADP+S+SR 
Sbjct: 121 RLVLLCSSPRYINTEGYEGGFEKSDIDQIIASIESNYQNWATHFPSLVVDEADPVSVSRF 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
           +KCLH+MRPEVA+ LAKTVF VDER+ILE VDIPCTI+ TK DI  PASVA+FMQ +IKG
Sbjct: 181 QKCLHEMRPEVALPLAKTVFGVDERDILEMVDIPCTIVQTKTDIVVPASVAVFMQNKIKG 240

Query: 241 SCTVRVINTNGHFPHLTAHLQLLQVLGEILGF 272
            CTV+VINT+GHFPHLTAHL+LLQVLG +LGF
Sbjct: 241 CCTVQVINTDGHFPHLTAHLELLQVLGTVLGF 271

BLAST of Cp4.1LG18g04340 vs. TAIR 10
Match: AT3G24420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 148/273 (54.21%), Postives = 200/273 (73.26%), Query Frame = 0

Query: 1   MVVMVENGPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFW 60
           MVV  +   L++A+NAK++GSG  +MVLAHGFG +QS+WD ++  LSQ ++V+VFDW F 
Sbjct: 1   MVVNQKISGLASAMNAKIIGSGERSMVLAHGFGGDQSVWDKIIPVLSQSFKVLVFDWLFS 60

Query: 61  GSIKDPNLYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQ 120
           G+IKD  LYDP KY+S   F++DLIAL++EL     +F+GHSMSG+IGC AS KRPDLF 
Sbjct: 61  GAIKDQTLYDPSKYNSLDVFSDDLIALMEELKFGPVVFVGHSMSGVIGCAASIKRPDLFT 120

Query: 121 RLILLCSSPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRL 180
            L+L+ +SPRYIN+EDY+GGF+  DI+ +I ++ SNYE WA  FSS VVD  D LS+ R 
Sbjct: 121 NLLLIAASPRYINSEDYKGGFESKDIDTIITSIGSNYEAWAVDFSSFVVDSRDSLSVQRF 180

Query: 181 RKCLHQMRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKG 240
            K L +M+PE A++LAK VF  DEREIL +V +PC ++    D+  P SVA FMQ++IKG
Sbjct: 181 EKSLKKMKPETALALAKIVFGSDEREILGQVSVPCHVIQPGNDVVVPVSVAYFMQEKIKG 240

Query: 241 SCTVRVI-NTNGHFPHLTAHLQLLQVLGEILGF 273
             TV +I +  GHFP +T+HL+LL V+  +L F
Sbjct: 241 KSTVEIIEDAIGHFPQMTSHLELLGVMRRLLEF 273

BLAST of Cp4.1LG18g04340 vs. TAIR 10
Match: AT3G03990.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 1.6e-56
Identity = 103/258 (39.92%), Postives = 167/258 (64.73%), Query Frame = 0

Query: 13  ALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPNLYDPQ 72
           ALN +V+G+G   + LAHGFGT+QS W  ++   +Q YRVV++D    GS+ +P+ +D  
Sbjct: 9   ALNVRVVGTGDRILFLAHGFGTDQSAWHLILPYFTQNYRVVLYDLVCAGSV-NPDYFDFN 68

Query: 73  KYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCSSPRYI 132
           +Y++   + +DL+ ++D LG+ +  ++GHS+S +IG IAS +RP+LF +LIL+  SPR++
Sbjct: 69  RYTTLDPYVDDLLNIVDSLGIQNCAYVGHSVSAMIGIIASIRRPELFSKLILIGFSPRFL 128

Query: 133 NTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVVDEADPLSLSRLRKCLHQMRPEVA 192
           N EDY GGF++ +IE++ + ME+NYE W   F+ L V    P ++    + L  MRP+++
Sbjct: 129 NDEDYHGGFEEGEIEKVFSAMEANYEAWVHGFAPLAVGADVPAAVREFSRTLFNMRPDIS 188

Query: 193 VSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRVINTNGH 252
           + +++TVF  D R +L  V +P  ++ T  D++ PASVA +++  + G  TV  + T GH
Sbjct: 189 LFVSRTVFNSDLRGVLGLVRVPTCVIQTAKDVSVPASVAEYLRSHLGGDTTVETLKTEGH 248

Query: 253 FPHLTAHLQLLQVLGEIL 271
            P L+A  QL Q L   L
Sbjct: 249 LPQLSAPAQLAQFLRRAL 265

BLAST of Cp4.1LG18g04340 vs. TAIR 10
Match: AT4G37470.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 211.8 bits (538), Expect = 6.7e-55
Identity = 101/260 (38.85%), Postives = 166/260 (63.85%), Query Frame = 0

Query: 8   GPLSTALNAKVMGSGREAMVLAHGFGTNQSLWDNMVGKLSQKYRVVVFDWTFWGSIKDPN 67
           G +  A N KV+GSG   +VL HGFGT+QS+W ++V  L   YRVV++D        +P+
Sbjct: 2   GVVEEAHNVKVIGSGEATIVLGHGFGTDQSVWKHLVPHLVDDYRVVLYD-NMGAGTTNPD 61

Query: 68  LYDPQKYSSYYAFAEDLIALLDELGLTSTIFLGHSMSGLIGCIASTKRPDLFQRLILLCS 127
            +D  +YS+   ++ DLIA+L++L + S IF+GHS+S +IG +AS  RPDLF +++++ +
Sbjct: 62  YFDFDRYSNLEGYSFDLIAILEDLKIESCIFVGHSVSAMIGVLASLNRPDLFSKIVMISA 121

Query: 128 SPRYINTEDYEGGFDQSDIEQMIANMESNYENWATQFSSLVV-DEADPLSLSRLRKCLHQ 187
           SPRY+N  DY+GGF+Q D+ Q+   + SNY+ W   F+ L V  + D +++    + L  
Sbjct: 122 SPRYVNDVDYQGGFEQEDLNQLFEAIRSNYKAWCLGFAPLAVGGDMDSIAVQEFSRTLFN 181

Query: 188 MRPEVAVSLAKTVFRVDEREILEKVDIPCTILHTKADIAAPASVAIFMQKQIKGSCTVRV 247
           MRP++A+S+ +T+F+ D R+IL  V +PC IL +  D+A P  V+ ++   +     V V
Sbjct: 182 MRPDIALSVGQTIFQSDMRQILPFVTVPCHILQSVKDLAVPVVVSEYLHANLGCESVVEV 241

Query: 248 INTNGHFPHLTAHLQLLQVL 267
           I ++GH P L++   ++ V+
Sbjct: 242 IPSDGHLPQLSSPDSVIPVI 260