Cp4.1LG18g04270 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG18g04270
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Vacuolar-processing enzyme
Location	Cp4.1LG18: 5384818 .. 5386947 (-)
RNA-Seq Expression	Cp4.1LG18g04270
Synteny	Cp4.1LG18g04270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGATACCTCAAACACACTCACATTTCCTCACCTAATATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATGCCGACCAACCCGGCAAGCGATGGGCGCTCCTCGTTGCTGGCTCTAATGGTTATGACAATTACCGACATCAGGTGCTCTTTATATCAATACATTATTCAATAAAGTTGTCACAGTGTGACTAACAGTTCTTCACTGTTGGATGTATACTGCTAATGTATTAACTCACTATCTTCTTGACGTAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTATGACGATATTGCGTTTGATCCAGACAATCCAAGGCCAGGAATCATTATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTCACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACTGACCATGGTGCTGCTGGAATGCTCGGTAATAACTTACATTTTCAACTCGTTTTTGTGTCAACAAACACTAAACTCCCCTCATGAATTTGACTATACATTGCTTCACATGATCATAGGAATGCCCATGGGTGACTATATTTACTCCAACGATCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAAAGTTACAAAAGCATGGTAATATATGTGGAGGCATGCGAGTCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGGTAACCCGTCGGCTTAAACTTTTGAGTTCAATGGCGTTTTAACACGGTACTTAATGAATTTGTGTTGTGTCGTGACATGTAGTGACAAGCATGATTCGAGCAAGGAAACTCTGAATCAACAATATCATACGGTAAGCTTATTGATTCTTAAGTAAGATCAGCTTAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACACTACTGATAAACATTTTCTCGTGTATTCCCAGGTTCGAAGAAGAACTTCGGTTGATAAAGTTGGGTACGGATCTCATGTAATGTTGTATGGAAATGAAAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATTCCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATATCATTTCTTCACTAGCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGGTATGAGTTGTTAAACAATGTATATATATTCTAATTACGATGGCAATCAACGTAGCCTTAACTTATAGAGCTTTGGTTTGATATCAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCGGCTAGAGGATGAAATTCTGAGTAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACCGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTAAGCCAATTTTCAAGTCCTATGCTTAGCTACACATACTTTATGCCATCAAAAACTCTGTTGATGGTGTCTCTGAAACACAGGTTAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACTCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAGCACCTGAAATGGGATCAATTTCTCGTAGTGTTAGCCTGCCTGTGCTAGGCGCCAGTCTTTATATATGTATAAAGAATCTGTAATGCTAATAATAAACTAATGATATTAATGTTAAACCAGTTAATCATCATCTCGATTGAATGAGAT

mRNA sequence

GGGATACCTCAAACACACTCACATTTCCTCACCTAATATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATGCCGACCAACCCGGCAAGCGATGGGCGCTCCTCGTTGCTGGCTCTAATGGTTATGACAATTACCGACATCAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTATGACGATATTGCGTTTGATCCAGACAATCCAAGGCCAGGAATCATTATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTCACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACTGACCATGGTGCTGCTGGAATGCTCGGAATGCCCATGGGTGACTATATTTACTCCAACGATCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAAAGTTACAAAAGCATGGTAATATATGTGGAGGCATGCGAGTCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATTCCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATATCATTTCTTCACTAGCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCGGCTAGAGGATGAAATTCTGAGTAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACCGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTTAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACTCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAGCACCTGAAATGGGATCAATTTCTCGTAGTGTTAGCCTGCCTGTGCTAGGCGCCAGTCTTTATATATGTATAAAGAATCTGTAATGCTAATAATAAACTAATGATATTAATGTTAAACCAGTTAATCATCATCTCGATTGAATGAGAT

Coding sequence (CDS)

ATGACAACTTTTGCTACAACTCCAACCACACTCCTCCTCATAGCTCTTCTGTTTTTCAACAGCATTATTGCTAAGAAACAGTGGGTCAGTCATGCCAATGCCGACCAACCCGGCAAGCGATGGGCGCTCCTCGTTGCTGGCTCTAATGGTTATGACAATTACCGACATCAGGCGGATGTATGTCATGCATATCAAATACTGAGGAAGGGTATCCCTGATGAGAACATAATCGTTTTCATGTATGACGATATTGCGTTTGATCCAGACAATCCAAGGCCAGGAATCATTATCAACAAACCAGGTGGGGCTGATGTTTACCAAGGAGTTCCCAAGGACTATACAGGAAAGGATGTGAATTCAGTCAACTTTTATGCTGCCATTTTGGGGAACAAATCTGGTGTCACTGGAGGAAGTGGTAAGGTTATTGATAGTGGGCCTAATGATCATATTTTCATCTACTACACTGACCATGGTGCTGCTGGAATGCTCGGAATGCCCATGGGTGACTATATTTACTCCAACGATCTAATGGACGTGCTGAAAAGGAAACATGAAGCCAAAAGTTACAAAAGCATGGTAATATATGTGGAGGCATGCGAGTCCGGGAGTATGTTCGAAGGATTGCTTCCAGAAAACATGAATATATATGCAACAACGGCATCAAATGCAACAGAGGATAGCTGGGCTGCATATTGTCCTGGACAGTCTCCTACTCCTCATACAGGCTATGATACTTGTCTCGGAGATTTGTACAGCGTCGCTTGGATGGAAGACAGCATTGGAAACAATTTCCTCAACACCTATTTTGGTAATTCCTCTGATGACAACAACAATGAATCCTCTGTTAAGAGTACTCATATCATTTCTTCACTAGCAAATGGGGTAAGCCAGCGTGATGCTAGCCTCCTTCATTACTGGTACAAGTTTCAAAATGCCCCATATGGGTCTAGAGAAAAGATGGAGGCTAGCGAGCGGCTAGAGGATGAAATTCTGAGTAGAAGGCATGCTGACAACAGTATAAATCAAATTGGTGAGCTTTTGTTTGGAGAAGCAAAGAGCTCACGAGTGCTTAACCGTATTAGGGGACATGGACAATCCTTCGTTGATGATTGGAACTGCTTTAAGAAATATGTTAAAATCTATGAGAAACATTGCAAAAGAATGTCAAGATATGGGATGAAATACACTCAAGCTCTAGCTAATATATGCAATGCTGGGATTACAATGGACCAAATGGATCAGGCTTGTCTCCAAACTTGCGTTGGTAGCACCTGA

Protein sequence

MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST

Homology

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot
Match: P49044 (Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 1.1e-130
Identity = 232/430 (53.95%), Postives = 299/430 (69.53%), Query Frame = 0


Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRP 94
           D  G RWA+L+AGSNGY NYRHQ+DVCHAYQ+LRK G  +ENIIVFMYDDIA + +NPRP
Sbjct: 51  DFEGTRWAILLAGSNGYWNYRHQSDVCHAYQLLRKGGSKEENIIVFMYDDIASNEENPRP 110

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IINKP G DVY GVPKDYTG +V++ NFYAA+LGNKS +TGGSGKV+DSGPNDHIF+Y
Sbjct: 111 GVIINKPDGDDVYAGVPKDYTGAEVHADNFYAALLGNKSALTGGSGKVVDSGPNDHIFVY 170

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENM 214
           YTDHG  G+LGMP+G Y+Y++DL +VLK+KH + +YKS+V Y+EACESGS+FEGLLP+++
Sbjct: 171 YTDHGGPGVLGMPVGPYLYASDLNEVLKKKHASGTYKSLVFYLEACESGSIFEGLLPDDL 230

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS-------------- 274
           NIYATTASNA E SW  YCPG  P P   Y TCLGDLYS+AWMEDS              
Sbjct: 231 NIYATTASNAEESSWGYYCPGDKPPPPPEYSTCLGDLYSIAWMEDSEVHNLQTESLQQQY 290

Query: 275 -----------------------IGNNFLNTYFG-NSSDDNNN-----ESSVKSTHIISS 334
                                  +  N L  Y G N ++DNN+     E+S+K    + +
Sbjct: 291 KLVKNRTISEPYGSHVMEYGDIGLSKNDLYQYLGTNPANDNNSFVDETENSLK----LRT 350

Query: 335 LANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGE 394
            +  V+QRDA L+H+W KF+ AP GS +K EA +++ + +  R+H DNS+  IG+LLFG 
Sbjct: 351 PSAAVNQRDADLIHFWEKFRKAPEGSSQKNEAEKQVLEAMSHRKHIDNSVKLIGQLLFGI 410

Query: 395 AKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMD 421
            K + +L+ +R  G   VD+W+C K  VK +E HC  +S+YGMK+ ++ ANICNAGI  +
Sbjct: 411 EKGTELLDVVRPAGSPLVDNWDCLKTMVKTFETHCGSLSQYGMKHMRSFANICNAGIPNE 470

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot
Match: Q39119 (Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g32940 PE=1 SV=2)

HSP 1 Score: 465.3 bits (1196), Expect = 7.3e-130
Identity = 229/424 (54.01%), Postives = 291/424 (68.63%), Query Frame = 0


Query: 38  GKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGII 97
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRK G+ +ENI+VFMYDDIA + +NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 98  INKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTD 157
           IN P G DVYQGVPKDYTG DVN  N +A ILG+K+ V GGSGKV+DSGPNDHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 158 HGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIY 217
           HG  G+LGMP   Y+Y+NDL DVLK+KH   +YKS+V Y+EACESGS+FEGLLPE +NIY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 218 ATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS----------------- 277
           ATTASNA E SW  YCPG+ P+P   Y+TCLGDLYSVAWMEDS                 
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 278 ----------------------IGNNFLNTYFG-NSSDDNNNESSVKSTHIISSLANGVS 337
                                 I  + L+ Y G N ++DN   +   S    S + N   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTN--- 356

Query: 338 QRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRV 397
           QRDA L+H+W K++ AP GS  K EA +++ + +  R H DNS+  +G++LFG ++   V
Sbjct: 357 QRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEV 416

Query: 398 LNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQAC 421
           LN++R  GQ  VDDWNC K  V+ +E+HC  +S+YG+K+ ++ ANICNAGI M+QM++A 
Sbjct: 417 LNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAA 476

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot
Match: P49043 (Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 9.0e-128
Identity = 227/426 (53.29%), Postives = 287/426 (67.37%), Query Frame = 0


Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRP 94
           D  G RWA+L+AGSNG+ NYRHQAD+CHAYQ+LRK G+ DENIIVFMYDDIAF+ +NPRP
Sbjct: 55  DSVGTRWAVLLAGSNGFWNYRHQADICHAYQLLRKGGLKDENIIVFMYDDIAFNEENPRP 114

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY+GVPKDYTG+DV    F+A +LGNK+ +TGGSGKV+DSGPNDHIFI+
Sbjct: 115 GVIINHPHGDDVYKGVPKDYTGEDVTVEKFFAVVLGNKTALTGGSGKVVDSGPNDHIFIF 174

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENM 214
           Y+DHG  G+LGMP   YIY+++L+DVLK+KH + +YKS+V Y+EACESGS+FEGLL E +
Sbjct: 175 YSDHGGPGVLGMPTSRYIYADELIDVLKKKHASGNYKSLVFYLEACESGSIFEGLLLEGL 234

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS-------------- 274
           NIYATTASNA E SW  YCPG+ P P   Y TCLGDLYS+AWMEDS              
Sbjct: 235 NIYATTASNAEESSWGTYCPGEIPGPPPEYSTCLGDLYSIAWMEDSDIHNLRTETLHQQY 294

Query: 275 ------------------------IGNNFLNTYFG-NSSDDNNNESSVKSTHIISSLANG 334
                                   +  N L TY G N ++DN       S   +   +  
Sbjct: 295 ELVKTRTASYNSYGSHVMQYGDIGLSKNNLFTYLGTNPANDNYTFVDENS---LRPASKA 354

Query: 335 VSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSS 394
           V+QRDA LLH+W K++ AP G+  K EA ++  + +  R H D+SI  IG+LLFG  K  
Sbjct: 355 VNQRDADLLHFWDKYRKAPEGTPRKAEAQKQFFEAMSHRMHVDHSIKLIGKLLFGIEKGP 414

Query: 395 RVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQ 421
            +LN +R  GQ  VDDW C K  V+ +E HC  +S+YGMK+ ++LANICN GI  ++M +
Sbjct: 415 EILNTVRPAGQPLVDDWGCLKSLVRTFESHCGALSQYGMKHMRSLANICNTGIGKEKMAE 474

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot
Match: O24325 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.5e-127
Identity = 230/464 (49.57%), Postives = 310/464 (66.81%), Query Frame = 0


Query: 6   TTPTTLLLIALLFFNSIIAKKQWV---------SHANADQ-PGKRWALLVAGSNGYDNYR 65
           T  T+LL + LLF  ++++  + +            N D   G RWA+L AGS+GY NYR
Sbjct: 5   TATTSLLALLLLFLVALVSAGRDLVGDFLRLPSDSGNGDNVHGTRWAILFAGSSGYWNYR 64

Query: 66  HQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYT 125
           HQAD+CHAYQ+LRK G+ DENIIVFMYDDIAF+ +NPR G+IIN P G +VY+GVPKDYT
Sbjct: 65  HQADICHAYQLLRKGGLKDENIIVFMYDDIAFNSENPRRGVIINSPNGDEVYKGVPKDYT 124

Query: 126 GKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSN 185
           G+DV + NFYAA+LG+KS +TGGSGKV++SGPNDHIFI+Y+DHG  G+LG P G YIY++
Sbjct: 125 GEDVTAHNFYAALLGDKSKLTGGSGKVVNSGPNDHIFIFYSDHGGPGVLGSPAGPYIYAS 184

Query: 186 DLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPG 245
           DL +VLK+KH + +YK++V Y+EACESGS+FEGLLPE++N+YATTASNA E SW  YCPG
Sbjct: 185 DLNEVLKKKHASGTYKNLVFYLEACESGSIFEGLLPEDINVYATTASNADESSWGTYCPG 244

Query: 246 QSPTPHTGYDTCLGDLYSVAWMEDSIGNNFL------------------NTYFGN----- 305
           + P+P   Y TCLGDLYSVAWMEDS  +N                      Y+G+     
Sbjct: 245 EDPSPPPEYSTCLGDLYSVAWMEDSDRHNLRTETLHQQYKLVKERTISGGLYYGSHVMQY 304

Query: 306 --------------SSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSR 365
                          +D  N   +    + + S +  V+QRDA L+H+W KF+ AP GS 
Sbjct: 305 GDVGLSKDILFHYLGTDPANENLTFVDENSLWSSSKAVNQRDADLVHFWDKFRKAPEGSP 364

Query: 366 EKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKY 422
           +K EA +++ + +  R H D+S+  +G+LLFG  K+  +LN +R  G + VDDW+C K  
Sbjct: 365 KKNEARKQVLEVMSHRMHIDDSVELVGKLLFGIEKAPELLNAVRPAGSALVDDWDCLKTM 424

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot
Match: P49047 (Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g25940 PE=2 SV=2)

HSP 1 Score: 452.2 bits (1162), Expect = 6.4e-126
Identity = 223/430 (51.86%), Postives = 293/430 (68.14%), Query Frame = 0


Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRP 94
           D    +WA+LVAGS+GY NYRHQADVCHAYQ+L+K G+ +ENI+VFMYDDIA + +NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY GVPKDYTG +VN  N  A ILGNK+ + GGSGKV+DSGPNDHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENM 214
           Y+DHG  G+LGMP    +Y+NDL DVLK+K+ + +YKS+V Y+EACESGS+FEGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNF--------- 274
           NIYATTASNA E SW  YCPG+ P+P + Y+TCLGDLYSVAW+EDS  +N          
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 275 ------------------------------LNTYFGNSSDDNN----NESSVKSTHIISS 334
                                         L  + G +  D N    NE+S++    ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 335 LANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGE 394
                +QRDA L+H+W+K+Q AP GS  K+EA +++ + +  R H DNSI  IG LLFG 
Sbjct: 339 -----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG- 398

Query: 395 AKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMD 421
            +   VLN++R  G+  VDDW+C K  V+ +E+HC  +S+YG+K+ +++AN+CNAGI M 
Sbjct: 399 LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMR 458

[Close]

BLAST of Cp4.1LG18g04270 vs. NCBI nr
Match: KAG6589920.1 (hypothetical protein SDJN03_15343, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 854 bits (2206), Expect = 1.22e-312
Identity = 414/424 (97.64%), Postives = 419/424 (98.82%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA  PDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDA 300
           TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGN+SDDNNNESSVKSTH ISSL+NGVSQRDA
Sbjct: 241 TGYDTCLGDLYSVAWMEDSIGNNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDA 300

Query: 301 SLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRI 360
           SLLHYWYKFQNAPYGSREKMEASE+LEDEILSRRHADNSINQIGELLFGEAKSSRVLN I
Sbjct: 301 SLLHYWYKFQNAPYGSREKMEASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSI 360

Query: 361 RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC 420
           RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC
Sbjct: 361 RGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTC 420

Query: 421 VGST 424
           VG+T
Sbjct: 421 VGNT 424

[Close]

BLAST of Cp4.1LG18g04270 vs. NCBI nr
Match: XP_023516171.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852 bits (2200), Expect = 4.40e-311
Identity = 424/463 (91.58%), Postives = 424/463 (91.58%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 300

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420
           ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY
Sbjct: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST
Sbjct: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 463

[Close]

BLAST of Cp4.1LG18g04270 vs. NCBI nr
Match: XP_022961313.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata])

HSP 1 Score: 836 bits (2159), Expect = 1.54e-304
Identity = 413/463 (89.20%), Postives = 421/463 (90.93%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA  PDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GS+FEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTHIISSL+NGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420
           ASERLEDEILSRRHADNS+NQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY
Sbjct: 379 ASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 438

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           EKHCKRMSRYGMKYTQALANICNAGIT+DQMD+ACLQTCVG+T
Sbjct: 439 EKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

[Close]

BLAST of Cp4.1LG18g04270 vs. NCBI nr
Match: XP_022987711.1 (vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima])

HSP 1 Score: 823 bits (2126), Expect = 8.32e-300
Identity = 408/463 (88.12%), Postives = 414/463 (89.42%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLI LLFFNSIIAKK+WVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIAF PDNPRPGIIINKPGGADVY GVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                        G
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESFG 300

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTHIISSL+N VSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNAVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420
           ASE+LEDEILSRRHADNSINQIG+LLFGEAKSSRVL R RGHGQSFVDDWNCFK YVKIY
Sbjct: 361 ASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCFKNYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           EKHCKRMSRYGMKYTQALAN CNAGITMDQMDQACLQTCVG+T
Sbjct: 421 EKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCVGNT 463

[Close]

BLAST of Cp4.1LG18g04270 vs. NCBI nr
Match: KAG7023589.1 (hypothetical protein SDJN02_14615, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 741 bits (1913), Expect = 2.25e-267
Identity = 369/417 (88.49%), Postives = 373/417 (89.45%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA  PDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTH ISSL+NGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHTISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYV 378
           ASE+LEDEILSRRHADNSINQIGELLFGEAKSSRVLN IRGHGQSFVDDWNCFKKYV
Sbjct: 379 ASEQLEDEILSRRHADNSINQIGELLFGEAKSSRVLNSIRGHGQSFVDDWNCFKKYV 435

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL
Match: A0A6J1HBF5 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=LOC111461846 PE=3 SV=1)

HSP 1 Score: 836 bits (2159), Expect = 7.44e-305
Identity = 413/463 (89.20%), Postives = 421/463 (90.93%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLIALLFFNSIIAKKQWVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 19  MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 78

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIA  PDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS
Sbjct: 79  CHAYQILRKGIPDENIIVFMYDDIALHPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 138

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 139 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 198

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACE+GS+FEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 199 KRKHEAKSYKSMVIYVEACEAGSLFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 258

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                       IG
Sbjct: 259 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESIG 318

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTHIISSL+NGVSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 319 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNGVSQRDASLLHYWYKFQNAPYGSREKME 378

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420
           ASERLEDEILSRRHADNS+NQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY
Sbjct: 379 ASERLEDEILSRRHADNSVNQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 438

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           EKHCKRMSRYGMKYTQALANICNAGIT+DQMD+ACLQTCVG+T
Sbjct: 439 EKHCKRMSRYGMKYTQALANICNAGITIDQMDKACLQTCVGNT 481

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL
Match: A0A6J1JF37 (vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC111485183 PE=3 SV=1)

HSP 1 Score: 823 bits (2126), Expect = 4.03e-300
Identity = 408/463 (88.12%), Postives = 414/463 (89.42%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADV 60
           MTTFATTPTTLLLI LLFFNSIIAKK+WVSHAN DQPGKRWALLVAGSNGYDNYRHQADV
Sbjct: 1   MTTFATTPTTLLLIPLLFFNSIIAKKEWVSHANTDQPGKRWALLVAGSNGYDNYRHQADV 60

Query: 61  CHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 120
           CHAYQILRKGIPDENIIVFMYDDIAF PDNPRPGIIINKPGGADVY GVPKDYTGKDVNS
Sbjct: 61  CHAYQILRKGIPDENIIVFMYDDIAFHPDNPRPGIIINKPGGADVYHGVPKDYTGKDVNS 120

Query: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180
           VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL
Sbjct: 121 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 180

Query: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240
           KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH
Sbjct: 181 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 240

Query: 241 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 300
           TGYDTCLGDLYSVAWMEDS                                        G
Sbjct: 241 TGYDTCLGDLYSVAWMEDSDKHDSSKETLNQQYHTVRRRTSVDKVGYGSHVMLYGNESFG 300

Query: 301 NNFLNTYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKME 360
           NNFLNTYFGN+SDDNNNESSVKSTHIISSL+N VSQRDASLLHYWYKFQNAPYGSREKME
Sbjct: 301 NNFLNTYFGNTSDDNNNESSVKSTHIISSLSNAVSQRDASLLHYWYKFQNAPYGSREKME 360

Query: 361 ASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIY 420
           ASE+LEDEILSRRHADNSINQIG+LLFGEAKSSRVL R RGHGQSFVDDWNCFK YVKIY
Sbjct: 361 ASEQLEDEILSRRHADNSINQIGDLLFGEAKSSRVLYRSRGHGQSFVDDWNCFKNYVKIY 420

Query: 421 EKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           EKHCKRMSRYGMKYTQALAN CNAGITMDQMDQACLQTCVG+T
Sbjct: 421 EKHCKRMSRYGMKYTQALANTCNAGITMDQMDQACLQTCVGNT 463

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL
Match: A0A6J1D475 (vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 635 bits (1639), Expect = 7.40e-226
Identity = 315/468 (67.31%), Postives = 369/468 (78.85%), Query Frame = 0


Query: 2   TTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADVC 61
           T +AT+   +LLIALL F+ IIAK++ V  A+ DQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62

Query: 62  HAYQILRKG-IPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 121
           HAYQILRKG +PDENIIVFMYDDIAF+P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122

Query: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182

Query: 182 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241
           K+KHEAKSYKSMVIYVEACESGSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242

Query: 242 TGYDTCLGDLYSVAWMEDS---------------------------------------IG 301
           T YDTCLGDLYS+AWMEDS                                       IG
Sbjct: 243 TDYDTCLGDLYSIAWMEDSDKHDLSKETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIG 302

Query: 302 NNFLNTYFGNSSDDNNNESSVKSTHIISS-----LANGVSQRDASLLHYWYKFQNAPYGS 361
           NN L+TY G + D+ N  SSV+ST  I+       +N VSQRDASL+HYW+KFQ AP+GS
Sbjct: 303 NNSLDTYIGANPDNYNYTSSVQSTDTIAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGS 362

Query: 362 REKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKK 421
           REK EA ++LEDEIL+RRH D+SI  I +LLFG+AKSS VLN +R  GQS VDDW CFKK
Sbjct: 363 REKTEARKQLEDEILNRRHVDSSIYHIAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKK 422

Query: 422 YVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
           +VK YEKHC+R+SRYGMKYT+ALANICNAGIT++QMDQACL+TC+  T
Sbjct: 423 FVKTYEKHCRRLSRYGMKYTRALANICNAGITINQMDQACLETCLVKT 470

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL
Match: A0A6J1D2Z4 (vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016825 PE=3 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 3.72e-220
Identity = 315/502 (62.75%), Postives = 369/502 (73.51%), Query Frame = 0


Query: 2   TTFATTPTTLLLIALLFFNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQADVC 61
           T +AT+   +LLIALL F+ IIAK++ V  A+ DQPGKRWA+LVAGS+GY+NYRHQADVC
Sbjct: 3   TCYATSTKFVLLIALLLFSDIIAKRESVDGASTDQPGKRWAILVAGSSGYENYRHQADVC 62

Query: 62  HAYQILRKG-IPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNS 121
           HAYQILRKG +PDENIIVFMYDDIAF+P NPRPG++INKP G DVYQGVPKDYTG+ VNS
Sbjct: 63  HAYQILRKGGLPDENIIVFMYDDIAFNPSNPRPGVVINKPDGVDVYQGVPKDYTGEHVNS 122

Query: 122 VNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVL 181
           +NFYA ILGN+S +TGGSGKV+DS  +DHIFIYYTDHG+AG+LGMP GDY+Y+ DLM+VL
Sbjct: 123 INFYAVILGNRSALTGGSGKVVDSDLHDHIFIYYTDHGSAGLLGMPEGDYVYAKDLMEVL 182

Query: 182 KRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPH 241
           K+KHEAKSYKSMVIYVEACESGSM EGLLPEN+ IYATTASNATE+SWA YCPGQ P+P 
Sbjct: 183 KQKHEAKSYKSMVIYVEACESGSMLEGLLPENIKIYATTASNATENSWATYCPGQFPSPP 242

Query: 242 TGYDTCLGDLYSVAWMED------------------------------------------ 301
           T YDTCLGDLYS+AWMED                                          
Sbjct: 243 TDYDTCLGDLYSIAWMEDRQYFLYTLCLYNKANLYLANPRHIKMNLCCAMICSDKHDLSK 302

Query: 302 -------------------------------SIGNNFLNTYFGNSSDDNNNESSVKSTHI 361
                                          SIGNN L+TY G + D+ N  SSV+ST  
Sbjct: 303 ETLIQQYDAVRRRTLVDKFGYGSHVMLYGNKSIGNNSLDTYIGANPDNYNYTSSVQSTDT 362

Query: 362 ISS-----LANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQ 421
           I+       +N VSQRDASL+HYW+KFQ AP+GSREK EA ++LEDEIL+RRH D+SI  
Sbjct: 363 IAPPSKLLYSNAVSQRDASLIHYWHKFQKAPFGSREKTEARKQLEDEILNRRHVDSSIYH 422

Query: 422 IGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANI 424
           I +LLFG+AKSS VLN +R  GQS VDDW CFKK+VK YEKHC+R+SRYGMKYT+ALANI
Sbjct: 423 IAKLLFGQAKSSEVLNNVRQQGQSLVDDWGCFKKFVKTYEKHCRRLSRYGMKYTRALANI 482

[Close]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL
Match: A0A5A7TXA7 (Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold114G00680 PE=3 SV=1)

HSP 1 Score: 615 bits (1587), Expect = 3.89e-218
Identity = 316/469 (67.38%), Postives = 354/469 (75.48%), Query Frame = 0


Query: 1   MTTFATTPTTLLLIALLF--FNSIIAKKQWVSHANADQPGKRWALLVAGSNGYDNYRHQA 60
           MTT    PT LL+I + F  FN+IIAK +W+S A  DQ GK WALL+AGSNGYDNYRHQ 
Sbjct: 1   MTTCDRNPTKLLIILITFLCFNNIIAKNEWISDATTDQTGKIWALLIAGSNGYDNYRHQG 60

Query: 61  DVCHAYQILRKGIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDV 120
                      GIPDENIIVFMYDDIAF P NPRPGIIINKP G DVY GVPKDYTGK V
Sbjct: 61  -----------GIPDENIIVFMYDDIAFHPSNPRPGIIINKPDGNDVYHGVPKDYTGKHV 120

Query: 121 NSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMD 180
           N+VNFYAAILGNKS +TGGSGKVI SGP DHIFIYY+DHGAAGMLGMP GDYI+ NDL+D
Sbjct: 121 NAVNFYAAILGNKSALTGGSGKVIHSGPQDHIFIYYSDHGAAGMLGMPEGDYIFVNDLID 180

Query: 181 VLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPT 240
           VLKRKH+AK+YKSMVIYVEACESGSMFEG+LPEN+ IYATTASNATEDSWA YCP QS  
Sbjct: 181 VLKRKHKAKNYKSMVIYVEACESGSMFEGVLPENIKIYATTASNATEDSWATYCPKQSHR 240

Query: 241 PHTGYDTCLGDLYSVAWMEDS--------------------------------------- 300
               Y+TCLGDL+SVAWMEDS                                       
Sbjct: 241 SPKDYNTCLGDLFSVAWMEDSDKHDLSKETLNQQYHAVRRRTFVDKSGYGSHVMLYGNKR 300

Query: 301 IGNNFLNTYFGNSSDDNNNESSVKSTHIISSLA----NGVSQRDASLLHYWYKFQNAPYG 360
           IGN+FL+T+ GNS D +N + SV+S   ISSL+    N VSQRDASL+HY YKFQ AP+G
Sbjct: 301 IGNDFLDTFLGNSPDYDNYKYSVQSPQTISSLSKLSSNSVSQRDASLIHYQYKFQEAPFG 360

Query: 361 SREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFK 420
           SREKMEA E+LEDEI  RRHADNSIN IG+LLFG+A+SS +L+ +R  GQS VDDW+CFK
Sbjct: 361 SREKMEAREQLEDEIFKRRHADNSINSIGKLLFGQARSSEILHNVRAQGQSLVDDWHCFK 420

Query: 421 KYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQACLQTCVGST 424
            +VKIYEKHC RMS YGMKYT+A+ANICNAGITM QMDQACLQTC+G T
Sbjct: 421 TFVKIYEKHCNRMSSYGMKYTRAIANICNAGITMHQMDQACLQTCLGKT 458

[Close]

BLAST of Cp4.1LG18g04270 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 465.3 bits (1196), Expect = 5.2e-131
Identity = 229/424 (54.01%), Postives = 291/424 (68.63%), Query Frame = 0


Query: 38  GKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGII 97
           G RWA+LVAGS+GY NYRHQAD+CHAYQ+LRK G+ +ENI+VFMYDDIA + +NPRPG I
Sbjct: 57  GTRWAVLVAGSSGYWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTI 116

Query: 98  INKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTD 157
           IN P G DVYQGVPKDYTG DVN  N +A ILG+K+ V GGSGKV+DSGPNDHIFI+Y+D
Sbjct: 117 INSPHGKDVYQGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSD 176

Query: 158 HGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIY 217
           HG  G+LGMP   Y+Y+NDL DVLK+KH   +YKS+V Y+EACESGS+FEGLLPE +NIY
Sbjct: 177 HGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIY 236

Query: 218 ATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDS----------------- 277
           ATTASNA E SW  YCPG+ P+P   Y+TCLGDLYSVAWMEDS                 
Sbjct: 237 ATTASNAEESSWGTYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELV 296

Query: 278 ----------------------IGNNFLNTYFG-NSSDDNNNESSVKSTHIISSLANGVS 337
                                 I  + L+ Y G N ++DN   +   S    S + N   
Sbjct: 297 KRRTAPVGYSYGSHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTN--- 356

Query: 338 QRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGEAKSSRV 397
           QRDA L+H+W K++ AP GS  K EA +++ + +  R H DNS+  +G++LFG ++   V
Sbjct: 357 QRDADLVHFWEKYRKAPEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEV 416

Query: 398 LNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMDQMDQAC 421
           LN++R  GQ  VDDWNC K  V+ +E+HC  +S+YG+K+ ++ ANICNAGI M+QM++A 
Sbjct: 417 LNKVRSAGQPLVDDWNCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAA 476

[Close]

BLAST of Cp4.1LG18g04270 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 452.2 bits (1162), Expect = 4.6e-127
Identity = 223/430 (51.86%), Postives = 293/430 (68.14%), Query Frame = 0


Query: 35  DQPGKRWALLVAGSNGYDNYRHQADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRP 94
           D    +WA+LVAGS+GY NYRHQADVCHAYQ+L+K G+ +ENI+VFMYDDIA + +NPRP
Sbjct: 39  DDDSTKWAVLVAGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRP 98

Query: 95  GIIINKPGGADVYQGVPKDYTGKDVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIY 154
           G+IIN P G DVY GVPKDYTG +VN  N  A ILGNK+ + GGSGKV+DSGPNDHIFIY
Sbjct: 99  GVIINSPNGEDVYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIY 158

Query: 155 YTDHGAAGMLGMPMGDYIYSNDLMDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENM 214
           Y+DHG  G+LGMP    +Y+NDL DVLK+K+ + +YKS+V Y+EACESGS+FEGLLPE +
Sbjct: 159 YSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGL 218

Query: 215 NIYATTASNATEDSWAAYCPGQSPTPHTGYDTCLGDLYSVAWMEDSIGNNF--------- 274
           NIYATTASNA E SW  YCPG+ P+P + Y+TCLGDLYSVAW+EDS  +N          
Sbjct: 219 NIYATTASNAEESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQY 278

Query: 275 ------------------------------LNTYFGNSSDDNN----NESSVKSTHIISS 334
                                         L  + G +  D N    NE+S++    ++ 
Sbjct: 279 ELVKKRTAGSGKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVT- 338

Query: 335 LANGVSQRDASLLHYWYKFQNAPYGSREKMEASERLEDEILSRRHADNSINQIGELLFGE 394
                +QRDA L+H+W+K+Q AP GS  K+EA +++ + +  R H DNSI  IG LLFG 
Sbjct: 339 -----NQRDADLVHFWHKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFG- 398

Query: 395 AKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCKRMSRYGMKYTQALANICNAGITMD 421
            +   VLN++R  G+  VDDW+C K  V+ +E+HC  +S+YG+K+ +++AN+CNAGI M 
Sbjct: 399 LEGHAVLNKVRPSGEPLVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMR 458

[Close]

BLAST of Cp4.1LG18g04270 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 420.2 bits (1079), Expect = 1.9e-117
Identity = 220/463 (47.52%), Postives = 289/463 (62.42%), Query Frame = 0


Query: 8   PTTLLLIALL--------FFNSIIAKKQWVSHANADQP--GKRWALLVAGSNGYDNYRHQ 67
           P  LLL+ LL        F   I+   +  + A+ D+   G RWA+LVAGS+GY NYRHQ
Sbjct: 9   PALLLLLVLLVHAESRGRFEPKILMPTEEANPADQDEDGVGTRWAVLVAGSSGYGNYRHQ 68

Query: 68  ADVCHAYQILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGK 127
           ADVCHAYQILRK G+ +ENI+V MYDDIA  P NPRPG +IN P G DVY GVPKDYTG 
Sbjct: 69  ADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPDGDDVYAGVPKDYTGS 128

Query: 128 DVNSVNFYAAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDL 187
            V + NFYA +LG++  V GGSGKVI S PNDHIF+YY DHG  G+LGMP   +IY+ D 
Sbjct: 129 SVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADF 188

Query: 188 MDVLKRKHEAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQS 247
           ++ LK+KH + +YK MVIYVEACESGS+FEG++P+++NIY TTASNA E S+  YCPG +
Sbjct: 189 IETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSYGTYCPGMN 248

Query: 248 PTPHTGYDTCLGDLYSVAWMEDSIGNNF------------------LNTYFGNSSDDNNN 307
           P+P + Y TCLGDLYSVAWMEDS  +N                    NTY G S      
Sbjct: 249 PSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVKMRTSNYNTYSGGSHVMEYG 308

Query: 308 ESSVKSTHI-------------------ISSLANGVSQRDASLLHYWYKFQNAPYGSREK 367
            +S+KS  +                   + S    V+QRDA LL  W+ ++ +  GSR+K
Sbjct: 309 NNSIKSEKLYLYQGFDPATVNLPLNELPVKSKIGVVNQRDADLLFLWHMYRTSEDGSRKK 368

Query: 368 MEASERLEDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVK 423
            +  + L +    R+H D S+  I  +LFG   +  VLN +R  G   VDDW C K  V+
Sbjct: 369 DDTLKELTETTRHRKHLDASVELIATILFG--PTMNVLNLVREPGLPLVDDWECLKSMVR 428

[Close]

BLAST of Cp4.1LG18g04270 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 406.0 bits (1042), Expect = 3.8e-113
Identity = 218/454 (48.02%), Postives = 278/454 (61.23%), Query Frame = 0


Query: 11  LLLIALLFFNSIIAKKQW-----VSHANADQPGKRWALLVAGSNGYDNYRHQADVCHAYQ 70
           + L ALL F++   K Q      V  ++    G RWA+LVAGSN Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 71  ILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFY 130
           ILRK G+ DENIIVFMYDDIAF  +NPRPG+IINKP G DVY+GVPKDYT + VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 131 AAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKH 190
             +LGN+SGVTGG+GKV+ SGPND+IFIYY DHGA G++ MP GD + + D  +VL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 191 EAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYD 250
           + K Y  MVIYVEACESGSMFEG+L +N+NIYA TA+N+ E SW  YCP   P P +   
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 251 TCLGDLYSVAWMEDS--------------------IGN------------------NFLN 310
           TCLGD +S++W+EDS                    +G+                  ++L+
Sbjct: 252 TCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLS 311

Query: 311 TYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKMEASERL 370
           +Y G  + +N+N +  +S     S +  V+ RD  LL+   K Q AP GS E  EA ++L
Sbjct: 312 SYIGR-NPENDNFTFTESFSSPISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKL 371

Query: 371 EDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCK 421
            DE   R+  D SI  I  L   +     +L   R  GQ  VDDW+CFK  V  ++ HC 
Sbjct: 372 LDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCG 431

[Close]

BLAST of Cp4.1LG18g04270 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 403.3 bits (1035), Expect = 2.4e-112
Identity = 217/452 (48.01%), Postives = 277/452 (61.28%), Query Frame = 0


Query: 11  LLLIALLFFNSIIAKKQW-----VSHANADQPGKRWALLVAGSNGYDNYRHQADVCHAYQ 70
           + L ALL F++   K Q      V  ++    G RWA+LVAGSN Y NYRHQAD+CHAYQ
Sbjct: 12  VFLHALLIFSAESRKTQLLNDNDVESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQ 71

Query: 71  ILRK-GIPDENIIVFMYDDIAFDPDNPRPGIIINKPGGADVYQGVPKDYTGKDVNSVNFY 130
           ILRK G+ DENIIVFMYDDIAF  +NPRPG+IINKP G DVY+GVPKDYT + VN  NFY
Sbjct: 72  ILRKGGLKDENIIVFMYDDIAFSSENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFY 131

Query: 131 AAILGNKSGVTGGSGKVIDSGPNDHIFIYYTDHGAAGMLGMPMGDYIYSNDLMDVLKRKH 190
             +LGN+SGVTGG+GKV+ SGPND+IFIYY DHGA G++ MP GD + + D  +VL++ H
Sbjct: 132 NVLLGNESGVTGGNGKVVKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMH 191

Query: 191 EAKSYKSMVIYVEACESGSMFEGLLPENMNIYATTASNATEDSWAAYCPGQSPTPHTGYD 250
           + K Y  MVIYVEACESGSMFEG+L +N+NIYA TA+N+ E SW  YCP   P P +   
Sbjct: 192 KRKKYNKMVIYVEACESGSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIG 251

Query: 251 TCLGDLYSVAWMEDS--------------------IGN------------------NFLN 310
           TCLGD +S++W+EDS                    +G+                  ++L+
Sbjct: 252 TCLGDTFSISWLEDSDLHDMSKETLEQQYHVVKRRVGSDVPETSHVCRFGTEKMLKDYLS 311

Query: 311 TYFGNSSDDNNNESSVKSTHIISSLANGVSQRDASLLHYWYKFQNAPYGSREKMEASERL 370
           +Y G  + +N+N +  +S     S +  V+ RD  LL+   K Q AP GS E  EA ++L
Sbjct: 312 SYIGR-NPENDNFTFTESFSSPISNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKL 371

Query: 371 EDEILSRRHADNSINQIGELLFGEAKSSRVLNRIRGHGQSFVDDWNCFKKYVKIYEKHCK 419
            DE   R+  D SI  I  L   +     +L   R  GQ  VDDW+CFK  V  ++ HC 
Sbjct: 372 LDEKNHRKQIDQSITDILRLSVKQTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCG 431

The following BLAST results are available for this feature:

BLAST of Cp4.1LG18g04270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49044	1.1e-130	53.95	Vacuolar-processing enzyme OS=Vicia sativa OX=3908 PE=1 SV=1	[more]
Q39119	7.3e-130	54.01	Vacuolar-processing enzyme gamma-isozyme OS=Arabidopsis thaliana OX=3702 GN=At4g...	[more]
P49043	9.0e-128	53.29	Vacuolar-processing enzyme OS=Citrus sinensis OX=2711 PE=2 SV=1	[more]
O24325	1.5e-127	49.57	Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=1 SV=1	[more]
P49047	6.4e-126	51.86	Vacuolar-processing enzyme alpha-isozyme OS=Arabidopsis thaliana OX=3702 GN=At2g...	[more]

BLAST of Cp4.1LG18g04270 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6589920.1	1.22e-312	97.64	hypothetical protein SDJN03_15343, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023516171.1	4.40e-311	91.58	vacuolar-processing enzyme gamma-isozyme-like [Cucurbita pepo subsp. pepo]	[more]
XP_022961313.1	1.54e-304	89.20	vacuolar-processing enzyme gamma-isozyme-like [Cucurbita moschata]	[more]
XP_022987711.1	8.32e-300	88.12	vacuolar-processing enzyme gamma-isozyme-like [Cucurbita maxima]	[more]
KAG7023589.1	2.25e-267	88.49	hypothetical protein SDJN02_14615, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Cp4.1LG18g04270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBF5	7.44e-305	89.20	vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1JF37	4.03e-300	88.12	vacuolar-processing enzyme gamma-isozyme-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1D475	7.40e-226	67.31	vacuolar-processing enzyme gamma-isozyme-like isoform X2 OS=Momordica charantia ...	[more]
A0A6J1D2Z4	3.72e-220	62.75	vacuolar-processing enzyme-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A5A7TXA7	3.89e-218	67.38	Vacuolar-processing enzyme gamma-isozyme-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of Cp4.1LG18g04270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32940.1	5.2e-131	54.01	gamma vacuolar processing enzyme	[more]
AT2G25940.1	4.6e-127	51.86	alpha-vacuolar processing enzyme	[more]
AT1G62710.1	1.9e-117	47.52	beta vacuolar processing enzyme	[more]
AT3G20210.1	3.8e-113	48.02	delta vacuolar processing enzyme	[more]
AT3G20210.2	2.4e-112	48.01	delta vacuolar processing enzyme	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001096	Peptidase C13, legumain	PRINTS	PR00776	HEMOGLOBNASE	coord: 112..127 score: 50.54 coord: 140..159 score: 64.57 coord: 189..205 score: 67.26 coord: 41..65 score: 75.48
IPR001096	Peptidase C13, legumain	PFAM	PF01650	Peptidase_C13	coord: 40..262 e-value: 5.3E-96 score: 321.1
IPR001096	Peptidase C13, legumain	PIRSF	PIRSF019663	Legumain	coord: 2..264 e-value: 1.8E-135 score: 450.4 coord: 259..424 e-value: 3.8E-42 score: 142.7
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 21..261 coord: 262..421
None	No IPR available	GENE3D	3.40.50.1460		coord: 37..299 e-value: 6.2E-101 score: 339.2
None	No IPR available	GENE3D	1.10.132.130		coord: 300..424 e-value: 7.1E-34 score: 118.6
None	No IPR available	PIRSR	PIRSR019663-1	PIRSR019663-1	coord: 38..260 e-value: 2.7E-129 score: 430.1
None	No IPR available	PANTHER	PTHR12000:SF45	VACUOLAR-PROCESSING ENZYME-LIKE	coord: 21..261 coord: 262..421
IPR043577	Asparaginyl endopeptidase	PIRSF	PIRSF500139	AE	coord: 259..424 e-value: 7.3E-42 score: 141.8 coord: 3..265 e-value: 1.8E-136 score: 453.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG18g04270.1	Cp4.1LG18g04270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008233	peptidase activity