Cp4.1LG18g04240 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g04240
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRNA methyltransferase
LocationCp4.1LG18: 5292614 .. 5296435 (+)
RNA-Seq ExpressionCp4.1LG18g04240
SyntenyCp4.1LG18g04240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGGTAGAAAAAAAGGAAGGAAAAGGACGAAAATGACCTATATACGCGCTGCCCATGGGTTTGCCGCGAAAATTAGGAAGCGCATAACAGTGCTAGGGGAAGCTGGTTTAAACCCTAGAGATATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTCTTCACTTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGGTATTTCTCCTGACCTATTCTTCCCCACTTACCTCGGAGTTCTAGGTTGCCCTTCTTGGGTCTTCACGTGGTAAATATAGTTCTGAGAAGTATTCTCGATTGTCTCCGGTTTCTGGTTAAGATTCGAACTATTATCGGTTAGTGATAGAAACGAGAATTTAAAATTTCGAGAATTGGTGTAGACCAGTTAAGCTTGTTTTTCCAACTCCAGTGCTCTAATAATTTATGATTCGTTCTTATTCGGCCGACAGAAATGTGCGTTACCCCTAGTTGCGTTTTCTTATTTTTTGTGTGGCAGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGGTAGCAATACTTCAATCTCAGTATTTATANTAAAAAAAAAAAAAAAAAAAAAGGATTCATACGTCCTTATCATGTGCAGTAATTTTATGAACTTCTTACTCATTTTACCAAAGGATATGCATGGAAGTCTCAAGTCTGAAAAGAAGTTTATATGCATGTTGCAGCAAAGACAATAAAACTATAGCAACTTCTTGTCATTTCGCTATGAACTTAAAACTGTCTTTTGTGCATCTCAGTTCAAGAAAGCTCTGTTATCACTGGCTATTTTATTGTTCATTGAAGATTGTGGAAGTGCTTTTTCTCAAATACTAGAGCTCTTTACTTATGCGGATAACTTTCTTTTCGCAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGGTAAGATTTACTCTAAAAGCTCTACAACTTCCATTGCAGATCCACTTTATACTTCTACAAAGTACATGAAAGCGGTTTTGTGAATAACTATCTTCTTTGGAGGTCTAAATCTTAATTGATGATGCATTCTATCATTAACTATTTTAAGCACTTGTAAATTAGTCATGTAAATTATAGCCTGGGATGGTTGGATGCTGATTTATTGTTCGACTATATTATTAGGGAGATTAGTTGGAGAATTCTTATACAAAAAATGTAATTGAAGGGTTTTACAGAAAAGGGTAGATATTAAAGGAGGTTGTTGATAAGGGTACGTGTGATACATATTTCGATCTAAATAGTCTTGAGTGGTATGCCCTTTCCCGGTCCCCTATGGGCTGTAATTATAACCTGTAACACGCATATTTTTTTGGCTTTGTCCTATCGGATTGCCTCTTAGTAACTTACTTCATGGGTGGGAACTCGAATCTCTGTTCCTCCTTTATATTGATAGAATCAAAGCTCGTGACCTGAAATTATTGAGGGAACTTACATTGTTTGTTGAACTACATGGATCATAGTGACTTCATCAATACGAACTCTATCTTTTACTGGTGGCTTTAGTAATAATCACATCATGTTCTGGTAGTTATGTTAGGAGAAAATATATCTCAAAAGTTAAAGAATTGTTTGTATACCAAGGTACTTAGCCAGAGTTGATCTCCCAGTCTTTCTATACAAATGATTGCTGAGTTTCCAACCTGAACTTCTCTTTATGCAAGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGAGAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGGTAAGTTAATCTTTTTATCTTAGGTGTTGTGTATTAATGCTATTTGTTTTCTTGACCCTTGATACATCTCCCTTCTGGGGTCACAATATGCGTTTTTTGTTTTTTTTTGTTTTTTCTGTACTGCAGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGTACTTGCCTTGTCATACACCTATGCACTTTGTGTTAAATGGTCCTAAGATCAATAATAACCATCCTAAGATCAATAATAACCTTGGTCAGTTATATGTTTGTATTTACTTTAATGTTCGATTAAGAACCTTCTTAATTATGCATTCATTCTGAAAAGGCAGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGGTAAAAAAATTCATGCATCCAAATTGTTAATCAATAATTACTTTTAGTCAAACTTGAATTTTTTTTTGGGTGCTCTTCTAGCATCTCATATCATATTGCCTTAGATCGCTGATCGTTCTAATGCAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGGTATTTTTATGATTTATTTGGCTTAGTTTTACAAACAGCATCAGTTTTCACTAACTTCTGGACAAAATACCACCCCAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTTAATCTGGACATCTGAACCAAATTGAATGCAGAGAGCCATCAGCTGGTCTTTAGTAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATATTCCAGACATGGTAAAGAGTGGCTGTTTTTGAAGGGTGCCGTCTGTAGTGGGATCCCTGTCACTTATGCGTAGGTGCTTTTTGAAGGCTTGTTGTAAGCTTCTGCTTTTTGATATTTACGTCGTTATCCTTTTGAACAATCTGAACGCTGATATATATTACTATTCATGTACCGTTCATTTATCATTTTCACTCATAACTAAGTTTAAACCTTTCTCTGTGTGACAACAAAGTTGTTTGTCTTGTTTTGTAACCCGAACAAGGTGACAGTTGATGAAAATGTAATCAAAATAAGTCTTTTAATTCTGCTCAGTTTGTATCAAAAAAGAAAAGAAAAGAAAATGTACTTCAAAAAGTGCTTTGCAATCCAAATAGTTCCCGAAGTGGATGCTAAATCGCTCTAAGATAAATTATCTTTAAGTTTCTTTAATTTTCTTTCAGGATATTATTATTTTGAACAAGTATTTAAACTTAGAAGTCTTGAAGAGGTTTTTAACATCTTGACTAGTCGTAGTCTGAGTGAATCCAATCCTATCCAGACTTCTTCCCAACCAAGCTCACCTGACTTGTCTTTGGTTGCAGGAATGGATCTTAGTCAATGATAGGGTTAGGAATGTCTTAGAGCAAAGTTAGGTGCAGATTAACCATTGGGTGTCCTTCACAGTTTCACAAGCTGGTCCTCCAGACAGCCATTGACAAATTCCGAGTTAAATATGGTTGATTTTGTAGCTATTTCATTTCCTCAACCACTAG

mRNA sequence

AAGGTAGAAAAAAAGGAAGGAAAAGGACGAAAATGACCTATATACGCGCTGCCCATGGGTTTGCCGCGAAAATTAGGAAGCGCATAACAGTGCTAGGGGAAGCTGGTTTAAACCCTAGAGATATTGGTTCCTCCATTGCATGCTGCCACCGCTTCCTCCGCCGGGGATTACCGTCGTTCATCGACGTTTCGTCTTCACTTCAGAGAGTATCAGCAAAAGAGATAATTAAAGTTGCGATTTAAGGAGATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGAGAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGAATGTTTGTTAATCTGGACATCTGAACCAAATTGAATGCAGAGAGCCATCAGCTGGTCTTTAGTAGTAGTTAGTTACAGGCCTTGTTTAGTTTAAATAGTACATTACATATTCCAGACATGGTAAAGAGTGGCTGTTTTTGAAGGGTGCCGTCTGTAGTGGGATCCCTGTCACTTATGCGTAGGAATGGATCTTAGTCAATGATAGGGTTAGGAATGTCTTAGAGCAAAGTTAGGTGCAGATTAACCATTGGGTGTCCTTCACAGTTTCACAAGCTGGTCCTCCAGACAGCCATTGACAAATTCCGAGTTAAATATGGTTGATTTTGTAGCTATTTCATTTCCTCAACCACTAG

Coding sequence (CDS)

ATGGCGGAAGAGCAGGAGAAAGCAGAAGCTGAACTCAAGCAAAATCCTAAAAGACGCAAGAAGCATGCTTTTCCCTATGGCAATTACCAAAGTTACTATGGATACCGGTTAGGTCAAAAATTTGTTGAAGATCCGCGTCTAAAGGTTTTCAAGAAAGAATGGTTTGAAGGCAAGGATTGCTTGGATATTGGTTGCAATAGTGGGATGGTTACCATTCAGATAGCTAGAAAGTTTAATTGCCGGAGGATTCTTGGCGTTGATATTGATCCCGATCGAGTTGACGATGCTTACCGGAATCTAAGAAGGGCAAGAATAAATGTTGGGAAGCCGGATAATGGTTTGGAGCTTGGTGCTTCTGGAGCTTCGAAGAGAGATACAGAAGCTTCTACAAGTATTTCTTCTTCCAAAGAACATGATCTTCATACCGATGTTTCCTTCCAACAGCAGAATTTTATATTTAGTCAATATCCACCTGAGAAGCACTATGATACAATTCTTTGTTTGAGTGTAGCAAAGTGGATTCATCTAAACTGGGGAGATGATGGTTTAATTACATTGTTTTACAAAATTTGGAGACTGCTTCACCCGGGTGGCATTCTTGTTTTGGAGCCTCAACCTTGGAAGTCTTATGAAGCCAACTATAGTGTTTCCGAGACAACTAAAAAGAACTTCAAGTCTATAAGGATCCTTCCAGAAGAGTTCCAAGAGATACTCCTAGACAAGATTGGGTTCAGAACAGTTGAAGCCTTAACCACCGGCTTGTCCCACAGCAATACAGGAGGATTCAATCGACCAATTTTTGTTTTCCAGAAGTGA

Protein sequence

MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASGASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Homology
BLAST of Cp4.1LG18g04240 vs. ExPASy Swiss-Prot
Match: Q6NPC9 (Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g51130 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 5.8e-80
Identity = 155/280 (55.36%), Postives = 196/280 (70.00%), Query Frame = 0

Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR--ARINVGKPD--NGLELGA 122
           IGCNSG++TI IA+KF CR ILGVDID  R++DA+ +LR+     N  KP        GA
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 SGASKRDTEASTSIS-------SSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVA 182
            G      E S S+S       S++  DL   VSFQ++NF+ ++   +  YDTILCLSV 
Sbjct: 161 DGV-HGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVT 220

Query: 183 KWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILP 242
           KW+HLNWGDDGLITLF KIWRLL PGGI V+EPQPWKSYE N  VSETT  N+++I + P
Sbjct: 221 KWVHLNWGDDGLITLFSKIWRLLQPGGIFVMEPQPWKSYENNRRVSETTAMNYRTIVLRP 280

Query: 243 EEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           + FQEILLDKIGFRTVE LT+ LS ++  GF+R I  FQK
Sbjct: 281 DRFQEILLDKIGFRTVEDLTSSLSGASK-GFDRQILAFQK 318

BLAST of Cp4.1LG18g04240 vs. ExPASy Swiss-Prot
Match: A3KQ55 (7SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV=2)

HSP 1 Score: 187.2 bits (474), Expect = 2.5e-46
Identity = 105/292 (35.96%), Postives = 153/292 (52.40%), Query Frame = 0

Query: 14  QNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQ 73
           QNP++ KK  F YGNY  YYGYR      EDPR++V   +WF GKD LD+GCN+G +T+ 
Sbjct: 352 QNPQQPKK-KFQYGNYNKYYGYR-NPGMSEDPRIRVMNPDWFRGKDVLDLGCNTGHLTLF 411

Query: 74  IARKFNCRRILGVDIDPDRVDDAYRNLRRARINV-----------GKPDNGLELGASGAS 133
           IA+ +    I+G+DID   +  A +N+R     V            K D G   G     
Sbjct: 412 IAKNWRPASIVGLDIDGSLIHAARQNIRHYLSEVQVQHSRRSGENTKADRGEVSGEEKDK 471

Query: 134 KRDTEAS-------------------TSISSSKEHDLHTDVSFQQQNFIFSQ----YPPE 193
            + ++ S                   T+  S    D   +V+F + N++           
Sbjct: 472 DKTSKHSFPVSLRISRGPIAGPPLPETNTHSLPPGDFPANVTFIKGNYVLESDVLLQTQR 531

Query: 194 KHYDTILCLSVAKWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSET 253
           + YD ILCLSV KW+HLNWGD GL   F+++++ L PGG+ +LEPQPW SY     ++E 
Sbjct: 532 EEYDVILCLSVTKWVHLNWGDAGLKRFFHRVYKHLRPGGLFILEPQPWSSYNKRKKLTEA 591

Query: 254 TKKNFKSIRILPEEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
             KN+ SIR+ P++F   L  ++GF + E + T  ++S   GF RPI ++ K
Sbjct: 592 ICKNYHSIRLKPDQFSSFLTTEVGFSSYELIGTSQNYSK--GFQRPISLYHK 639

BLAST of Cp4.1LG18g04240 vs. ExPASy Swiss-Prot
Match: Q8K3A9 (7SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 SV=2)

HSP 1 Score: 184.9 bits (468), Expect = 1.2e-45
Identity = 107/281 (38.08%), Postives = 157/281 (55.87%), Query Frame = 0

Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF+G+D LD+GCN G +T+ IA K
Sbjct: 384 KKQQLKFQYGNYCKYYGYR--NPSCEDVRLRVLKPEWFQGRDVLDLGCNVGHLTLSIACK 443

Query: 78  FNCRRILGVDIDPDRVDDAYRNLR---------RARINVGKPDNGLELGASGASKRD--- 137
           +   R++G+DIDP  +  A +N+R         +A+ + G P    E G     KR    
Sbjct: 444 WGPARMVGLDIDPRLIHSARQNIRHYLSEELRLQAQTSEGDPGTEGEEGTITVRKRSCFP 503

Query: 138 ---TEASTSISSSKEHDLHTDVSFQQQNFIF--SQYPPEK---------HYDTILCLSVA 197
              T +   I++ +      D S    N +F    Y  ++          YD +LC S+ 
Sbjct: 504 ASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDELVDAQRPEYDVVLCFSLT 563

Query: 198 KWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILP 257
           KW+HLNWGD+GL  +F +I+R L PGGILVLEPQPW SY    S++ET  KN+  I++ P
Sbjct: 564 KWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYCKRKSLTETIYKNYFRIQLKP 623

Query: 258 EEFQEILLD-KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           E+F   L   ++GF + E + T   ++ + GF RP+++F K
Sbjct: 624 EQFSSYLTSPEVGFSSYELVAT--PNNTSRGFQRPVYLFHK 660

BLAST of Cp4.1LG18g04240 vs. ExPASy Swiss-Prot
Match: Q7L2J0 (7SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 7.9e-45
Identity = 106/281 (37.72%), Postives = 153/281 (54.45%), Query Frame = 0

Query: 18  RRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDIGCNSGMVTIQIARK 77
           ++++  F YGNY  YYGYR      ED RL+V K EWF G+D LD+GCN G +T+ IA K
Sbjct: 407 KKQQRKFQYGNYCKYYGYR--NPSCEDGRLRVLKPEWFRGRDVLDLGCNVGHLTLSIACK 466

Query: 78  FNCRRILGVDIDPDRVDDAYRNLRR---------ARINVGKPDNGLELGASGASKRD--- 137
           +   R++G+DID   +  A +N+R           +   G P    E G +   KR    
Sbjct: 467 WGPSRMVGLDIDSRLIHSARQNIRHYLSEELRLPPQTLEGDPGAEGEEGTTTVRKRSCFP 526

Query: 138 ---TEASTSISSSKEHDLHTDVSFQQQNFIF--SQYPPEK---------HYDTILCLSVA 197
              T +   I++ +      D S    N +F    Y  ++          YD +LCLS+ 
Sbjct: 527 ASLTASRGPIAAPQVPLDGADTSVFPNNVVFVTGNYVLDRDDLVEAQTPEYDVVLCLSLT 586

Query: 198 KWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILP 257
           KW+HLNWGD+GL  +F +I+R L PGGILVLEPQPW SY    +++ET  KN+  I++ P
Sbjct: 587 KWVHLNWGDEGLKRMFRRIYRHLRPGGILVLEPQPWSSYGKRKTLTETIYKNYYRIQLKP 646

Query: 258 EEFQEILLD-KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           E+F   L    +GF + E + T   H+ + GF RP+++F K
Sbjct: 647 EQFSSYLTSPDVGFSSYELVAT--PHNTSKGFQRPVYLFHK 683

BLAST of Cp4.1LG18g04240 vs. ExPASy Swiss-Prot
Match: Q9U2R0 (Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y17G7B.18 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 5.1e-44
Identity = 104/278 (37.41%), Postives = 160/278 (57.55%), Query Frame = 0

Query: 4   EQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLDI 63
           ++EK+ AE ++         + YGN+  YYG RL      D RL VF+K+WFE K  LDI
Sbjct: 97  KEEKSAAENRKQKTEYFNKKYRYGNFDRYYGIRLNPG-ESDKRLSVFQKDWFEHKQALDI 156

Query: 64  GCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLR-----RARINVGKPDN-GLELG 123
           GCN+G +T+ IA+ F+ RRI+G+DID   +  A +N+R        ++   P + G++ G
Sbjct: 157 GCNAGFLTLSIAKDFSPRRIIGIDIDEHLIGVARKNIRHYCDHETEVSGKFPASFGVQFG 216

Query: 124 ASGASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPP----EKHYDTILCLSVAKW 183
               S+R+ EA  S S+        ++ F+++N++          +  +D IL LS+ KW
Sbjct: 217 T--VSQRN-EAPRSFSTK----FPDNIWFKKENYVLESDEMLDMIQPEFDVILALSITKW 276

Query: 184 IHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEE 243
           IHLNWGDDG+   F + +  LHPGG L++EPQ + SY+    +SE  K N+  I   PE+
Sbjct: 277 IHLNWGDDGMRRFFRRAYAQLHPGGRLIIEPQAFDSYKKRAKMSEELKANYSKIEFKPED 336

Query: 244 FQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           F+  L++ +GF +VE L  G+  + + GF RPI V+ K
Sbjct: 337 FEMWLIETVGFESVEKL--GVVGAKSKGFERPIDVYLK 364

BLAST of Cp4.1LG18g04240 vs. NCBI nr
Match: XP_023516346.1 (probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558 bits (1438), Expect = 9.43e-201
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271

BLAST of Cp4.1LG18g04240 vs. NCBI nr
Match: XP_022961298.1 (probable RNA methyltransferase At5g51130 [Cucurbita moschata])

HSP 1 Score: 555 bits (1430), Expect = 1.57e-199
Identity = 270/271 (99.63%), Postives = 270/271 (99.63%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASK DTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271

BLAST of Cp4.1LG18g04240 vs. NCBI nr
Match: KAG6589938.1 (putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 555 bits (1430), Expect = 3.80e-199
Identity = 270/271 (99.63%), Postives = 270/271 (99.63%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASK DTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271

BLAST of Cp4.1LG18g04240 vs. NCBI nr
Match: XP_022987832.1 (probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita maxima])

HSP 1 Score: 550 bits (1418), Expect = 1.06e-197
Identity = 267/271 (98.52%), Postives = 269/271 (99.26%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELKQNPKRRKKHAFPYGNY+SYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKQNPKRRKKHAFPYGNYRSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASK DTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVF+K
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFRK 271

BLAST of Cp4.1LG18g04240 vs. NCBI nr
Match: XP_038878262.1 (probable RNA methyltransferase At5g51130 isoform X2 [Benincasa hispida])

HSP 1 Score: 453 bits (1166), Expect = 2.71e-159
Identity = 224/272 (82.35%), Postives = 245/272 (90.07%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELK+NPK+R+KHAFPYGNYQ+YYGYR+GQ  VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKKNPKKRRKHAFPYGNYQNYYGYRVGQNLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRA-RINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIAR+FNCR ILGVDID DRV+DAY NLRRA R+  G P+NG + GAS
Sbjct: 61  LDIGCNNGIVTIQIARRFNCRSILGVDIDSDRVEDAYWNLRRATRLGDGNPNNG-KRGAS 120

Query: 121 GASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ D EA TSISSSKEHDLHT VSF++QNFIFS +PPEK YDTILCLSVAKWIHLNWG
Sbjct: 121 RASRIDLEACTSISSSKEHDLHTVVSFKRQNFIFSHHPPEKRYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLITLF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NFK+I+I PEEFQEILL
Sbjct: 181 DDGLITLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFKTIKIRPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           DKIGFRTVEALT GLS SN+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALTLGLSDSNSGGFNRPILVFQK 271

BLAST of Cp4.1LG18g04240 vs. ExPASy TrEMBL
Match: A0A6J1HBE1 (RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1)

HSP 1 Score: 555 bits (1430), Expect = 7.58e-200
Identity = 270/271 (99.63%), Postives = 270/271 (99.63%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASK DTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271

BLAST of Cp4.1LG18g04240 vs. ExPASy TrEMBL
Match: A0A6J1JBF8 (RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 5.12e-198
Identity = 267/271 (98.52%), Postives = 269/271 (99.26%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAEEQEK EAELKQNPKRRKKHAFPYGNY+SYYGYRLGQKFVEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEEQEKTEAELKQNPKRRKKHAFPYGNYRSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120
           LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG
Sbjct: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRARINVGKPDNGLELGASG 120

Query: 121 ASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180
           ASK DTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD
Sbjct: 121 ASKIDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWGD 180

Query: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240
           DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD
Sbjct: 181 DGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILLD 240

Query: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           KIGFRTVEALTTGLSHSNTGGFNRPIFVF+K
Sbjct: 241 KIGFRTVEALTTGLSHSNTGGFNRPIFVFRK 271

BLAST of Cp4.1LG18g04240 vs. ExPASy TrEMBL
Match: A0A5D3BG34 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold369G00080 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 4.53e-158
Identity = 218/272 (80.15%), Postives = 243/272 (89.34%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRA-RINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ D EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

BLAST of Cp4.1LG18g04240 vs. ExPASy TrEMBL
Match: A0A1S3CJ98 (RNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103501041 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 4.53e-158
Identity = 218/272 (80.15%), Postives = 243/272 (89.34%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRA-RINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ D EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

BLAST of Cp4.1LG18g04240 vs. ExPASy TrEMBL
Match: A0A5A7V9R8 (RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79G001350 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 2.93e-157
Identity = 218/272 (80.15%), Postives = 243/272 (89.34%), Query Frame = 0

Query: 1   MAEEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDC 60
           MAE+Q++ EAE KQNPK+ +KHAFPYGNY++YYGYR+GQK VEDPRLKVFKKEWFEGKDC
Sbjct: 1   MAEDQDQTEAERKQNPKKHRKHAFPYGNYRNYYGYRVGQKLVEDPRLKVFKKEWFEGKDC 60

Query: 61  LDIGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRRA-RINVGKPDNGLELGAS 120
           LDIGCN+G+VTIQIARKFNCR ILGVDID DRVDDAY NLR+  R++ G PDNGL+  AS
Sbjct: 61  LDIGCNNGIVTIQIARKFNCRSILGVDIDSDRVDDAYWNLRKEIRLSDGNPDNGLKHCAS 120

Query: 121 GASKRDTEASTSISSSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVAKWIHLNWG 180
            AS+ D EASTSISSSKEHDLH  VSF++QNFIFS +PP+KHYDTILCLSVAKWIHLNWG
Sbjct: 121 KASRIDLEASTSISSSKEHDLHAAVSFKRQNFIFSHHPPDKHYDTILCLSVAKWIHLNWG 180

Query: 181 DDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILPEEFQEILL 240
           DDGLI LF KIW+LL+ GGILVLEPQPWKSYE NYSVSETTK NF++I+ILPEEFQEILL
Sbjct: 181 DDGLIRLFSKIWKLLNLGGILVLEPQPWKSYETNYSVSETTKMNFRTIKILPEEFQEILL 240

Query: 241 DKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 271
           DKIGFRTVEAL  GLS  N+GGFNRPI VFQK
Sbjct: 241 DKIGFRTVEALNLGLSDGNSGGFNRPILVFQK 272

BLAST of Cp4.1LG18g04240 vs. TAIR 10
Match: AT5G51130.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.1e-81
Identity = 155/280 (55.36%), Postives = 196/280 (70.00%), Query Frame = 0

Query: 3   EEQEKAEAELKQNPKRRKKHAFPYGNYQSYYGYRLGQKFVEDPRLKVFKKEWFEGKDCLD 62
           ++Q+  +    Q+ K++ +  +P+GNY++YYGYR+     EDPRLKV KKEWFEGKDCLD
Sbjct: 41  KQQQGQQGNCNQSKKKKNQEVYPFGNYRNYYGYRISNDTDEDPRLKVLKKEWFEGKDCLD 100

Query: 63  IGCNSGMVTIQIARKFNCRRILGVDIDPDRVDDAYRNLRR--ARINVGKPD--NGLELGA 122
           IGCNSG++TI IA+KF CR ILGVDID  R++DA+ +LR+     N  KP        GA
Sbjct: 101 IGCNSGIMTIHIAKKFGCRSILGVDIDTSRIEDAHWHLRKFVRMQNSTKPSEKKSSSEGA 160

Query: 123 SGASKRDTEASTSIS-------SSKEHDLHTDVSFQQQNFIFSQYPPEKHYDTILCLSVA 182
            G      E S S+S       S++  DL   VSFQ++NF+ ++   +  YDTILCLSV 
Sbjct: 161 DGV-HGSKEPSVSLSNGEAKADSAETKDLSQIVSFQKENFVQTRNLDDNRYDTILCLSVT 220

Query: 183 KWIHLNWGDDGLITLFYKIWRLLHPGGILVLEPQPWKSYEANYSVSETTKKNFKSIRILP 242
           KW+HLNWGDDGLITLF KIWRLL PGGI V+EPQPWKSYE N  VSETT  N+++I + P
Sbjct: 221 KWVHLNWGDDGLITLFSKIWRLLQPGGIFVMEPQPWKSYENNRRVSETTAMNYRTIVLRP 280

Query: 243 EEFQEILLDKIGFRTVEALTTGLSHSNTGGFNRPIFVFQK 272
           + FQEILLDKIGFRTVE LT+ LS ++  GF+R I  FQK
Sbjct: 281 DRFQEILLDKIGFRTVEDLTSSLSGASK-GFDRQILAFQK 318

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NPC95.8e-8055.36Probable RNA methyltransferase At5g51130 OS=Arabidopsis thaliana OX=3702 GN=At5g... [more]
A3KQ552.5e-4635.967SK snRNA methylphosphate capping enzyme OS=Danio rerio OX=7955 GN=mepce PE=3 SV... [more]
Q8K3A91.2e-4538.087SK snRNA methylphosphate capping enzyme OS=Mus musculus OX=10090 GN=Mepce PE=1 ... [more]
Q7L2J07.9e-4537.727SK snRNA methylphosphate capping enzyme OS=Homo sapiens OX=9606 GN=MEPCE PE=1 S... [more]
Q9U2R05.1e-4437.41Probable RNA methyltransferase Y17G7B.18 OS=Caenorhabditis elegans OX=6239 GN=Y1... [more]
Match NameE-valueIdentityDescription
XP_023516346.19.43e-201100.00probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022961298.11.57e-19999.63probable RNA methyltransferase At5g51130 [Cucurbita moschata][more]
KAG6589938.13.80e-19999.63putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022987832.11.06e-19798.52probable RNA methyltransferase At5g51130 isoform X1 [Cucurbita maxima][more]
XP_038878262.12.71e-15982.35probable RNA methyltransferase At5g51130 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HBE17.58e-20099.63RNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461838 PE=3 SV=1[more]
A0A6J1JBF85.12e-19898.52RNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111485259 PE=3 SV=1[more]
A0A5D3BG344.53e-15880.15RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold36... [more]
A0A1S3CJ984.53e-15880.15RNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103501041 PE=3 SV=1[more]
A0A5A7V9R82.93e-15780.15RNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold79... [more]
Match NameE-valueIdentityDescription
AT5G51130.14.1e-8155.36S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041698Methyltransferase domain 25PFAMPF13649Methyltransf_25coord: 61..107
e-value: 3.5E-6
score: 27.6
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 22..271
e-value: 1.9E-74
score: 252.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 114..140
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availablePANTHERPTHR12315:SF07SK SNRNA METHYLPHOSPHATE CAPPING ENZYMEcoord: 4..271
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 61..203
e-value: 1.36007E-6
score: 43.9579
IPR010675RNA methyltransferase bin3, C-terminalPFAMPF06859Bin3coord: 162..271
e-value: 8.7E-40
score: 135.7
IPR039772RNA methyltransferase Bin3-likePANTHERPTHR12315BICOID-INTERACTING PROTEIN RELATEDcoord: 4..271
IPR024160Bin3-type S-adenosyl-L-methionine binding domainPROSITEPS51515BIN3_SAMcoord: 44..271
score: 74.389977
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 25..209

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g04240.1Cp4.1LG18g04240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity