Cp4.1LG18g03370 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG18g03370
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Threonine synthase
Location	Cp4.1LG18: 4839316 .. 4841309 (+)
RNA-Seq Expression	Cp4.1LG18g03370
Synteny	Cp4.1LG18g03370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGATCGACACAAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGTATCCCAAAACCTTGCCACCATCCAGCCAATGGTGGCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTCACTAATTTGATAACCATGGACGTTGTACGGTTCGAACGCGGACGGTGGAGGGATGTTGGGAGTGGGCCTTCAGCGGGCCTTTTTTTTCTTTGTAATCGTGCTCGTTTTGCTAGGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAAAGCAGCCCTGTTTTAAGACATTATCGCCGCCGCCGCTGCCGCCGCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAATTTCCGCAATCCCATTCTCAGAATCCGACGACCATCCGCTTCTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAAGCTTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCCGATGATATCGTCAGCGCCTTCGAAGGTAACTCGAACCTATTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTCAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCACTCTCGGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGTTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTGGCCAATTCCTTGAATAGTTTGAGGCTCGAAGGGCAAAAGACTGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCCGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAAAATTCGAATGGCATCGTCGAGGAAGCAACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGGGTCATCAGCCCGACCGACAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCAAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGGCTCCAATGTCTAAAGTGTAG

mRNA sequence

ATGATGATCGACACAAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGTATCCCAAAACCTTGCCACCATCCAGCCAATGGTGGCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTCACTAATTTGATAACCATGGACGTTGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAAAGCAGCCCTGTTTTAAGACATTATCGCCGCCGCCGCTGCCGCCGCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAATTTCCGCAATCCCATTCTCAGAATCCGACGACCATCCGCTTCTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAAGCTTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCCGATGATATCGTCAGCGCCTTCGAAGGTAACTCGAACCTATTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTCAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCACTCTCGGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGTTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTGGCCAATTCCTTGAATAGTTTGAGGCTCGAAGGGCAAAAGACTGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCCGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAAAATTCGAATGGCATCGTCGAGGAAGCAACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGGGTCATCAGCCCGACCGACAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCAAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGGCTCCAATGTCTAAAGTGTAG

Coding sequence (CDS)

ATGATGATCGACACAAGGTCACCCATCATCTGTCTCATAGCCAACACATTGGCTGGCCTCGAGCCCATGATGAGCCATGTATCCCAAAACCTTGCCACCATCCAGCCAATGGTGGCGAGGTCTCAGCTTGGGCTCGAACACAGTCGACTGGTTCGTTGGAGAGCAAAGCTCATGGACACAAGGTTCACTAATTTGATAACCATGGACGTTGTTAAAAGGAAGGTCTGGAAGTGGAGTTATAATCAAAGCAGCCCTGTTTTAAGACATTATCGCCGCCGCCGCTGCCGCCGCCGCCTCAGAATGGCTGCCTCTTCGCTGTTAAACCCCGCCATTTCTTTCCCCTCTTACAAGCCCAAGCTCCAAGCCAACCCAAATTTCCGCAATCCCATTCTCAGAATCCGACGACCATCCGCTTCTTCCGATTCGTCTTCCTCCACGCCGACTAATGGCTCTCCGTCATTGTCGACCACCAAGAACCGCCGCTCTGCCGACGAGAACATCAAAGACGAAGCACGGCGCCATCGTGCAGCCTCCGGAGAAGACCGTAGCAACTTCTCGGCCAAGTACGTGCCATTTAACGCCGGCGCCGACTCGTCTGAGTGGTACTCGCTTGACGAGGTCGTTTACCGGAGCCGATCTGGTGGATTGCTCGACGTTCAGCACGACATGGAAGCTTTGAAGAAATTCGACGGCGCGTACTGGCGCAACCTCTTCGATTCACGCGTGGGGAAAACCACATGGCCTTATGGGTCCGGTGTGTGGAGCAAGAAGGAGTGGGTGTTGCCGGAGATTGACCCCGATGATATCGTCAGCGCCTTCGAAGGTAACTCGAACCTATTTTGGGCTGAGAGATTTGGCAAACAGTTTCTGGGTATGAATGATTTATGGGTAAAACACTGTGGGATTAGCCACACTGGGAGTTTCAAGGATTTGGGCATGACTGTTTTGGTCAGCCAAGTGAACCGGCTGAGGAAATTGAAACGCCCCGTCGTCGGAGTCGGTTGTGCATCGACCGGAGACACATCGGCCGCACTCTCGGCCTACTGCGCATCTGCAGGGATCCCTTCAATCGTCTTCCTCCCTGCAAACAAGATTTCCATGGCACAGTTAGTTCAGCCCATTGCAAATGGAGCCTTTGTGTTGAGTATAGACACTGATTTTGATGGTTGCATGAAGCTAATTAGGGAAGTTACAGCAGAATTGCCCATTTACTTGGCCAATTCCTTGAATAGTTTGAGGCTCGAAGGGCAAAAGACTGCTGCCATTGAAATTCTACAGCAGTTTGATTGGGCAGTACCTGAATGGGTGATCATTCCAGGAGGCAATCTTGGGAACATATATGCATTTTACAAAGGATTCAAGATGTGCCAAGAGCTTGGCCTTGTTGACAGGATTCCTAGGCTTGTTTGTGCACAAGCAGCCAATGCTAACCCTCTGTATTTACACTACAAATCCGGGTGGAAAGATTTCAAGGCTGTGAAAGCAAGTTCGACGTTCGCCTCGGCCATTCAGATTGGGGATCCTGTTTCCATAGATAGAGCTGTTTGTGCTCTACAAAATTCGAATGGCATCGTCGAGGAAGCAACAGAGGAGGAACTAATGGATGCAATGGCACAAGCTGACTCTACTGGGATGTTCATTTGCCCTCACACAGGGGTGGCACTAACGGCTTTGATCAAGCTGAGAAACCAAGGGGTCATCAGCCCGACCGACAGGACGGTGGTGGTGAGCACTGCTCATGGCCTGAAGTTTACACAGTCAAAAATCGACTATCACTCCAAGGAAATCCCAGACATGGCTTGCCAATTTGCTAACCCACCTGTTCAAGTGAAGGCTGATTATGGGAGTGTCATGGATGTACTTAAAGATTATTTACTACAAAAGGCTCCAATGTCTAAAGTGTAG

Protein sequence

MMIDTRSPIICLIANTLAGLEPMMSHVSQNLATIQPMVARSQLGLEHSRLVRWRAKLMDTRFTNLITMDVVKRKVWKWSYNQSSPVLRHYRRRRCRRRLRMAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV

Homology

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BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot
Match: Q9MT28 (Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 865.1 bits (2234), Expect = 4.8e-250
Identity = 429/498 (86.14%), Postives = 456/498 (91.57%), Query Frame = 0


Query: 143 SSSTPTNG------------SPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYV 202
           S+S P NG            + ++S  K+RR ADENI++EARRH ++      NFSA+YV
Sbjct: 23  STSKPNNGIHFFTPIKATATNDAISQQKHRRPADENIREEARRHCSS-----HNFSARYV 82

Query: 203 PFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYG 262
           PFNAG +S EWYSLDE+VYRSRSGGLLDVQHDM+ALKKFDG YWR+LFDSRVGKTTWPYG
Sbjct: 83  PFNAGPNSDEWYSLDEIVYRSRSGGLLDVQHDMDALKKFDGQYWRSLFDSRVGKTTWPYG 142

Query: 263 SGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKD 322
           SGVWSKKEWVLPEID DDIVSAFEGNSNLFWAERFGKQFLGM DLWVKHCGISHTGSFKD
Sbjct: 143 SGVWSKKEWVLPEIDSDDIVSAFEGNSNLFWAERFGKQFLGMTDLWVKHCGISHTGSFKD 202

Query: 323 LGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL 382
           LGMTVLVSQVNRLRK+ +PVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL
Sbjct: 203 LGMTVLVSQVNRLRKMHKPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL 262

Query: 383 VQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW 442
           VQPIANGAFVLSIDTDFDGCM+LIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW
Sbjct: 263 VQPIANGAFVLSIDTDFDGCMQLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW 322

Query: 443 AVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF 502
            VPEWVI+PGGNLGNIYAFYKGF+MC+ELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF
Sbjct: 323 EVPEWVIVPGGNLGNIYAFYKGFQMCKELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF 382

Query: 503 KAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPH 562
           K VKA++TFASAIQIGDPVSIDRAV ALQ  NGIVEEATEEELMDAMAQADSTGMFICPH
Sbjct: 383 KPVKANTTFASAIQIGDPVSIDRAVFALQQCNGIVEEATEEELMDAMAQADSTGMFICPH 442

Query: 563 TGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQV 622
           TGVALTAL KLRN GVI+PTDRTVVVSTAHGLKFTQSKIDYHSKEI DM C+FANPPV+V
Sbjct: 443 TGVALTALFKLRNSGVIAPTDRTVVVSTAHGLKFTQSKIDYHSKEIKDMECRFANPPVEV 502

Query: 623 KADYGSVMDVLKDYLLQK 629
           KAD+GSVMDVLK YLL +
Sbjct: 503 KADFGSVMDVLKSYLLSQ 515

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BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot
Match: Q9S7B5 (Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 5.4e-246
Identity = 430/525 (81.90%), Postives = 464/525 (88.38%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA+S L N ++S  S  PK       R+  L   RP   S ++       +P  +  K  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK-APIETAVKPP 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
              ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLLDV+
Sbjct: 61  HRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVE 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV AL+ 
Sbjct: 361 LVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKK 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
            NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVSTAH
Sbjct: 421 CNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 626
           GLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 GLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot
Match: Q9SSP5 (Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 SV=1)

HSP 1 Score: 799.7 bits (2064), Expect = 2.5e-230
Identity = 413/531 (77.78%), Postives = 447/531 (84.18%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSY-----KPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLS 160
           MA+ SL + A  FPS+     KP   A+         +R  SAS      TP        
Sbjct: 1   MASFSLPHSATYFPSHSETSLKPHSAAS-------FTVRCTSASPAVPPQTP-------- 60

Query: 161 TTKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGG 220
             K RRS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG 
Sbjct: 61  -QKPRRSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGA 120

Query: 221 LLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEG 280
           LLDVQHD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEG
Sbjct: 121 LLDVQHDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEG 180

Query: 281 NSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGC 340
           NSNLFWAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGC
Sbjct: 181 NSNLFWAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGC 240

Query: 341 ASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIR 400
           ASTGDTSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIR
Sbjct: 241 ASTGDTSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIR 300

Query: 401 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKM 460
           EVTAELPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF M
Sbjct: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHM 360

Query: 461 CQELGLVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRA 520
           C+ELGLVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRA
Sbjct: 361 CKELGLVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRA 420

Query: 521 VCALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTV 580
           V AL+ SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTV
Sbjct: 421 VYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTV 480

Query: 581 VVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 626
           VVSTAHGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 VVSTAHGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot
Match: Q58860 (Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=thrC PE=3 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 6.1e-72
Identity = 157/423 (37.12%), Postives = 242/423 (57.21%), Query Frame = 0


Query: 202 YSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVL 261
           Y +DE++Y    GGLL++ +D E +K         + + ++ K       GVW   E+ L
Sbjct: 12  YDVDEIIYTCECGGLLEIIYDYEEIKD-------KVSEEKLRKRE----IGVWRYLEY-L 71

Query: 262 PEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVN 321
           P  D   IVS  EG + L+      K+ LG+ +L+VK+ G + TGSFKD GMTV V++ N
Sbjct: 72  PVKDESKIVSLCEGGTPLYRCNNLEKE-LGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 131

Query: 322 RLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVL 381
            L      V  VGCASTG+TSA+L+AY A +G   IV LP  K+++ +L Q +  GA V+
Sbjct: 132 EL-----GVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVI 191

Query: 382 SIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGG 441
            +  +FD  + +++++  E  IYL NS+N  RLEGQKT A EI  Q +W VP+ VI+P G
Sbjct: 192 QVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVG 251

Query: 442 NLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFAS 501
           N GNI A +KGFK  +  G++D +P++   QA  A P+   ++   KD    K   T A+
Sbjct: 252 NAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIAT 311

Query: 502 AIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAM-AQADSTGMFICPHTGVALTALIK 561
           AI+IG+PV+  +A+ A+ +S G  E  T+EE+++A    A   G+F+ P +  ++  L K
Sbjct: 312 AIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKK 371

Query: 562 LRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDV 621
           L  +G+I   +R V ++T HGLK             PD A + +  P++++ D   +  +
Sbjct: 372 LLEEGIIDRDERIVCITTGHGLK------------DPDAAIRASEEPIKIECDMNVLKRI 404

Query: 622 LKD 624
           LK+
Sbjct: 432 LKE 404

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot
Match: Q8TQD4 (Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=thrC PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 9.5e-65
Identity = 152/391 (38.87%), Postives = 227/391 (58.06%), Query Frame = 0


Query: 202 YSLDEVVYR-SRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWV 261
           YS DEV+Y  S+  GLLDV +D  ++K           D    KT  P    VW   + +
Sbjct: 13  YSRDEVIYTCSKCDGLLDVIYDYSSIK----------IDMEKLKTECP---SVWKYAKLL 72

Query: 262 LPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQV 321
             E +P   V+  EG + L+  +R  ++ +G+  L+VKH G++ TGSFKD GMTV V+  
Sbjct: 73  PVEREP---VTIQEGGTPLYKCDRLAEK-IGIKKLYVKHEGMNPTGSFKDRGMTVGVT-- 132

Query: 322 NRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFV 381
              + L+  +  V CASTG+TSAAL+ Y A AGIP +V LPA K+++ ++ Q + +GA V
Sbjct: 133 ---KALELGMNTVACASTGNTSAALAIYGAKAGIPVVVLLPAGKVALGKVAQALMHGAKV 192

Query: 382 LSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPG 441
           LSI  +FD  + L+R + ++  IYL NS+N  RLEGQKT   EI  Q D+ VP+ +++P 
Sbjct: 193 LSIRGNFDDALALVRTLCSQEKIYLLNSINPYRLEGQKTIGFEIADQLDFKVPDRIVLPV 252

Query: 442 GNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFA 501
           GN GNI A YKGF+  + LG+ D +P++   QA  + P+    KSG       +   T A
Sbjct: 253 GNAGNITAIYKGFREFKILGITDSLPKMTGIQAEGSCPIVKAIKSGAPAITPEENPETVA 312

Query: 502 SAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQ-ADSTGMFICPHTGVALTALI 561
           +AI+IG+PV+  +A+ A++ S G  E  T+EE++ A    A   G+ + P +  ++  L 
Sbjct: 313 TAIRIGNPVNATKALSAIRESGGTAESVTDEEILAAQKDLARLEGIGVEPASAASVAGLR 372

Query: 562 KLRNQGVISPTDRTVVVSTAHGLKFTQSKID 591
           KL + GVI   +  V ++T H LK  Q+ ID
Sbjct: 373 KLVDMGVIGRDETVVCITTGHLLKDPQTVID 381

[Close]

BLAST of Cp4.1LG18g03370 vs. NCBI nr
Match: KAG6590018.1 (hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1103 bits (2854), Expect = 0.0
Identity = 554/564 (98.23%), Postives = 556/564 (98.58%), Query Frame = 0


Query: 71  VKRKVWKWSYNQSSPVLRHYRRRRCRRRLRMAASSLLNPAISFPSYKPKLQANPNFRNPI 130
           VKRKVWKWSYNQ S VLRHYRRRR R RLRMAASSLLNPAISFPSYKPKLQANP FRNPI
Sbjct: 67  VKRKVWKWSYNQRSLVLRHYRRRRLRLRLRMAASSLLNPAISFPSYKPKLQANPKFRNPI 126

Query: 131 LRIRRPSASSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYV 190
           L I R SA+SDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYV
Sbjct: 127 LGIGRVSATSDSSSSTPTNGSPSLSTTKNRRSADENIKDEARRHRAASGEDRSNFSAKYV 186

Query: 191 PFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYG 250
           PFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYG
Sbjct: 187 PFNAGADSSEWYSLDEVVYRSRSGGLLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYG 246

Query: 251 SGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKD 310
           SGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKD
Sbjct: 247 SGVWSKKEWVLPEIDPDDIVSAFEGNSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKD 306

Query: 311 LGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL 370
           LGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL
Sbjct: 307 LGMTVLVSQVNRLRKLKRPVVGVGCASTGDTSAALSAYCASAGIPSIVFLPANKISMAQL 366

Query: 371 VQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW 430
           VQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW
Sbjct: 367 VQPIANGAFVLSIDTDFDGCMKLIREVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDW 426

Query: 431 AVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF 490
           AVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF
Sbjct: 427 AVPEWVIIPGGNLGNIYAFYKGFKMCQELGLVDRIPRLVCAQAANANPLYLHYKSGWKDF 486

Query: 491 KAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPH 550
           KAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPH
Sbjct: 487 KAVKASSTFASAIQIGDPVSIDRAVCALQNSNGIVEEATEEELMDAMAQADSTGMFICPH 546

Query: 551 TGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQV 610
           TGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQV
Sbjct: 547 TGVALTALIKLRNQGVISPTDRTVVVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQV 606

Query: 611 KADYGSVMDVLKDYLLQKAPMSKV 634
           KADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 607 KADYGSVMDVLKDYLLQKSPMSKV 630

[Close]

BLAST of Cp4.1LG18g03370 vs. NCBI nr
Match: XP_023515876.1 (threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1068 bits (2762), Expect = 0.0
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. NCBI nr
Match: XP_022987673.1 (threonine synthase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1067 bits (2759), Expect = 0.0
Identity = 533/534 (99.81%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. NCBI nr
Match: XP_022960888.1 (threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetical protein SDJN02_14707, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 529/534 (99.06%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MAASSLLNPAISFPSYKPKLQANP FRNPILRI R SA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. NCBI nr
Match: XP_038878968.1 (threonine synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1023 bits (2644), Expect = 0.0
Identity = 507/534 (94.94%), Postives = 519/534 (97.19%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA SSLLNPAISFPS KPKLQ NP FRNP+LRI + S++SDSSSS PTNGSP LST KNR
Sbjct: 1   MATSSLLNPAISFPSRKPKLQPNPKFRNPVLRIPKASSTSDSSSSPPTNGSPPLSTLKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADS EWYSLDE++YRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSPEWYSLDEIIYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL+KLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALVKLRNKGIIRPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSK+IPDMACQFANPPVQVKADYGSVMDVLKDYLL KAP S+V
Sbjct: 481 GLKFTQSKIDYHSKDIPDMACQFANPPVQVKADYGSVMDVLKDYLLNKAPKSQV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL
Match: A0A6J1JK49 (Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1)

HSP 1 Score: 1067 bits (2759), Expect = 0.0
Identity = 533/534 (99.81%), Postives = 534/534 (100.00%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL
Match: A0A6J1HAA4 (Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 529/534 (99.06%), Postives = 531/534 (99.44%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MAASSLLNPAISFPSYKPKLQANP FRNPILRI R SA+SDSSSSTPTNGSPSLSTTKNR
Sbjct: 1   MAASSLLNPAISFPSYKPKLQANPKFRNPILRIGRVSATSDSSSSTPTNGSPSLSTTKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQK+PMSKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKSPMSKV 534

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL
Match: A0A5D3CDZ3 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001840 PE=3 SV=1)

HSP 1 Score: 1004 bits (2597), Expect = 0.0
Identity = 501/534 (93.82%), Postives = 512/534 (95.88%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA SSL NP ISFPS  PKLQ+ P FRNPILRI  P A+SDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISFPSRNPKLQSTPKFRNPILRI--PKATSDSSPSPPTNGSPPLSTLKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRA+ ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAIYALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL KAP SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL
Match: A0A5A7UKY2 (Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G001890 PE=3 SV=1)

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 501/534 (93.82%), Postives = 511/534 (95.69%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA  SL NP ISFPS  PKLQ+ P FRNPILRI  P A+SDSS S PTNGSP LST KNR
Sbjct: 1   MATYSLFNPTISFPSRNPKLQSTPKFRNPILRI--PKATSDSSPSPPTNGSPPLSTLKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKF QSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL KAP SKV
Sbjct: 481 GLKFAQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNKAPKSKV 532

[Close]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL
Match: A0A0A0M223 (Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1)

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 501/534 (93.82%), Postives = 511/534 (95.69%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA SSL NP IS PS  PKLQ+ P FRNPILRI  P A+SDSS S PTNGSP LST KNR
Sbjct: 1   MATSSLFNPTISLPSRNPKLQSTPKFRNPILRI--PKATSDSSPSPPTNGSPPLSTLKNR 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
           RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAG DS+E YSLDE+VYRSRSGGLLD+Q
Sbjct: 61  RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGPDSTECYSLDEIVYRSRSGGLLDIQ 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALKK+DGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF
Sbjct: 121 HDMEALKKYDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VPEWVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWGVPEWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAV ALQN
Sbjct: 361 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVYALQN 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
           SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRN+G+I PTDRTVVVSTAH
Sbjct: 421 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNKGIIGPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYLLQKAPMSKV 634
           GLKFTQSKIDYHSKEIPDMACQFANPPVQVKAD+GSVMDVLKDYLL  AP SKV
Sbjct: 481 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADFGSVMDVLKDYLLNSAPKSKV 532

[Close]

BLAST of Cp4.1LG18g03370 vs. TAIR 10
Match: AT4G29840.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 851.7 bits (2199), Expect = 3.9e-247
Identity = 430/525 (81.90%), Postives = 464/525 (88.38%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSYKPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLSTTKNR 160
           MA+S L N ++S  S  PK       R+  L   RP   S ++       +P  +  K  
Sbjct: 1   MASSCLFNASVS--SLNPKQDPIRRHRSTSLLRHRPVVISCTADGNNIK-APIETAVKPP 60

Query: 161 RSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGGLLDVQ 220
              ++NI+DEARR+R+ +    + FSAKYVPFNA   S+E YSLDE+VYRSRSGGLLDV+
Sbjct: 61  HRTEDNIRDEARRNRSNA---VNPFSAKYVPFNAAPGSTESYSLDEIVYRSRSGGLLDVE 120

Query: 221 HDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEGNSNLF 280
           HDMEALK+FDGAYWR+LFDSRVGK+TWPYGSGVWSKKEWVLPEID DDIVSAFEGNSNLF
Sbjct: 121 HDMEALKRFDGAYWRDLFDSRVGKSTWPYGSGVWSKKEWVLPEIDDDDIVSAFEGNSNLF 180

Query: 281 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGCASTGD 340
           WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRK+KRPVVGVGCASTGD
Sbjct: 181 WAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKMKRPVVGVGCASTGD 240

Query: 341 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREVTAE 400
           TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIRE+TAE
Sbjct: 241 TSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIREITAE 300

Query: 401 LPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKMCQELG 460
           LPIYLANSLNSLRLEGQKTAAIEILQQFDW VP+WVI+PGGNLGNIYAFYKGFKMCQELG
Sbjct: 301 LPIYLANSLNSLRLEGQKTAAIEILQQFDWQVPDWVIVPGGNLGNIYAFYKGFKMCQELG 360

Query: 461 LVDRIPRLVCAQAANANPLYLHYKSGWKDFKAVKASSTFASAIQIGDPVSIDRAVCALQN 520
           LVDRIPR+VCAQAANANPLYLHYKSGWKDFK + AS+TFASAIQIGDPVSIDRAV AL+ 
Sbjct: 361 LVDRIPRMVCAQAANANPLYLHYKSGWKDFKPMTASTTFASAIQIGDPVSIDRAVYALKK 420

Query: 521 SNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTVVVSTAH 580
            NGIVEEATEEELMDAMAQADSTGMFICPHTGVALTAL KLRNQGVI+PTDRTVVVSTAH
Sbjct: 421 CNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALFKLRNQGVIAPTDRTVVVSTAH 480

Query: 581 GLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 626
           GLKFTQSKIDYHS  IPDMAC+F+NPPV VKAD+G+VMDVLK YL
Sbjct: 481 GLKFTQSKIDYHSNAIPDMACRFSNPPVDVKADFGAVMDVLKSYL 519

[Close]

BLAST of Cp4.1LG18g03370 vs. TAIR 10
Match: AT1G72810.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 799.7 bits (2064), Expect = 1.7e-231
Identity = 413/531 (77.78%), Postives = 447/531 (84.18%), Query Frame = 0


Query: 101 MAASSLLNPAISFPSY-----KPKLQANPNFRNPILRIRRPSASSDSSSSTPTNGSPSLS 160
           MA+ SL + A  FPS+     KP   A+         +R  SAS      TP        
Sbjct: 1   MASFSLPHSATYFPSHSETSLKPHSAAS-------FTVRCTSASPAVPPQTP-------- 60

Query: 161 TTKNRRSADENIKDEARRHRAASGEDRSNFSAKYVPFNAGADSSEWYSLDEVVYRSRSGG 220
             K RRS DENI+DEARR          N SA+YVPFNA   S+E YSLDE+VYRS+SG 
Sbjct: 61  -QKPRRSPDENIRDEARRR----PHQLQNLSARYVPFNAPPSSTESYSLDEIVYRSQSGA 120

Query: 221 LLDVQHDMEALKKFDGAYWRNLFDSRVGKTTWPYGSGVWSKKEWVLPEIDPDDIVSAFEG 280
           LLDVQHD  ALK++DG +WRNLFDSRVGKT WPYGSGVWSKKEWVLPEID DDIVSAFEG
Sbjct: 121 LLDVQHDFAALKRYDGEFWRNLFDSRVGKTNWPYGSGVWSKKEWVLPEIDDDDIVSAFEG 180

Query: 281 NSNLFWAERFGKQFLGMNDLWVKHCGISHTGSFKDLGMTVLVSQVNRLRKLKRPVVGVGC 340
           NSNLFWAERFGKQ+L MNDLWVKHCGISHTGSFKDLGM+VLVSQVNRLRK+ +PV+GVGC
Sbjct: 181 NSNLFWAERFGKQYLQMNDLWVKHCGISHTGSFKDLGMSVLVSQVNRLRKMNKPVIGVGC 240

Query: 341 ASTGDTSAALSAYCASAGIPSIVFLPANKISMAQLVQPIANGAFVLSIDTDFDGCMKLIR 400
           ASTGDTSAALSAYCASAGIPSIVFLPA+KISMAQLVQPIANGAFVLSIDTDFDGCM LIR
Sbjct: 241 ASTGDTSAALSAYCASAGIPSIVFLPADKISMAQLVQPIANGAFVLSIDTDFDGCMHLIR 300

Query: 401 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFDWAVPEWVIIPGGNLGNIYAFYKGFKM 460
           EVTAELPIYLANSLNSLRLEGQKTAAIEILQQF+W VP+WVI+PGGNLGNIYAFYKGF M
Sbjct: 301 EVTAELPIYLANSLNSLRLEGQKTAAIEILQQFNWQVPDWVIVPGGNLGNIYAFYKGFHM 360

Query: 461 CQELGLVDRIPRLVCAQAANANPLYLHYKSGWK-DFKAVKASSTFASAIQIGDPVSIDRA 520
           C+ELGLVDRIPRLVCAQAANANPLYLHYKSG+K DF  +KA++TFASAIQIGDPVSIDRA
Sbjct: 361 CKELGLVDRIPRLVCAQAANANPLYLHYKSGFKEDFNPLKANTTFASAIQIGDPVSIDRA 420

Query: 521 VCALQNSNGIVEEATEEELMDAMAQADSTGMFICPHTGVALTALIKLRNQGVISPTDRTV 580
           V AL+ SNGIVEEATEEELMDA A ADSTGMFICPHTGVALTAL+KLR  GVI   DRTV
Sbjct: 421 VYALKKSNGIVEEATEEELMDATALADSTGMFICPHTGVALTALMKLRKSGVIGANDRTV 480

Query: 581 VVSTAHGLKFTQSKIDYHSKEIPDMACQFANPPVQVKADYGSVMDVLKDYL 626
           VVSTAHGLKFTQSKIDYHSK I +MAC+ ANPPV+VKA +GSVMDVLK+YL
Sbjct: 481 VVSTAHGLKFTQSKIDYHSKNIKEMACRLANPPVKVKAKFGSVMDVLKEYL 511

The following BLAST results are available for this feature:

BLAST of Cp4.1LG18g03370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MT28	4.8e-250	86.14	Threonine synthase, chloroplastic OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]
Q9S7B5	5.4e-246	81.90	Threonine synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS1 PE=1 ...	[more]
Q9SSP5	2.5e-230	77.78	Threonine synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TS2 PE=1 ...	[more]
Q58860	6.1e-72	37.12	Threonine synthase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 266...	[more]
Q8TQD4	9.5e-65	38.87	Threonine synthase OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 /...	[more]

BLAST of Cp4.1LG18g03370 vs. NCBI nr


Match Name	Identity	Description
KAG6590018.1	98.23	hypothetical protein SDJN03_15441, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023515876.1	100.00	threonine synthase, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022987673.1	99.81	threonine synthase, chloroplastic [Cucurbita maxima]	[more]
XP_022960888.1	99.06	threonine synthase, chloroplastic [Cucurbita moschata] >KAG7023681.1 hypothetica...	[more]
XP_038878968.1	94.94	threonine synthase, chloroplastic-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG18g03370 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1JK49	99.81	Threonine synthase OS=Cucurbita maxima OX=3661 GN=LOC111485156 PE=3 SV=1	[more]
A0A6J1HAA4	99.06	Threonine synthase OS=Cucurbita moschata OX=3662 GN=LOC111461551 PE=3 SV=1	[more]
A0A5D3CDZ3	93.82	Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00...	[more]
A0A5A7UKY2	93.82	Threonine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold181G0...	[more]
A0A0A0M223	93.82	Threonine synthase OS=Cucumis sativus OX=3659 GN=Csa_1G590270 PE=3 SV=1	[more]

BLAST of Cp4.1LG18g03370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29840.1	3.9e-247	81.90	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]
AT1G72810.1	1.7e-231	77.78	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 272..578 e-value: 1.8E-47 score: 162.2
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 161..383 e-value: 7.4E-64 score: 218.0
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 384..631 e-value: 1.5E-70 score: 239.9
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 184..624
IPR004450	Threonine synthase-like	TIGRFAM	TIGR00260	TIGR00260	coord: 260..583 e-value: 2.1E-60 score: 202.6
None	No IPR available	PIRSR	PIRSR038945-1	PIRSR038945-1	coord: 252..566 e-value: 2.7E-70 score: 234.9
None	No IPR available	PIRSR	PIRSR038945-2	PIRSR038945-2	coord: 252..566 e-value: 2.7E-70 score: 234.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 134..180
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 161..180
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 135..160
None	No IPR available	PANTHER	PTHR10314:SF176	THREONINE SYNTHASE, CHLOROPLASTIC-LIKE ISOFORM X1	coord: 132..628
None	No IPR available	PANTHER	PTHR10314	CYSTATHIONINE BETA-SYNTHASE	coord: 132..628
None	No IPR available	CDD	cd01563	Thr-synth_1	coord: 254..584 e-value: 4.39623E-158 score: 455.129
IPR000634	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	PROSITE	PS00165	DEHYDRATASE_SER_THR	coord: 299..313

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG18g03370.1	Cp4.1LG18g03370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019344	cysteine biosynthetic process
biological_process	GO:0009088	threonine biosynthetic process
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0004795	threonine synthase activity