Cp4.1LG18g02820 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g02820
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial
LocationCp4.1LG18: 4346872 .. 4352454 (+)
RNA-Seq ExpressionCp4.1LG18g02820
SyntenyCp4.1LG18g02820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTATTATTACATTTTTAATTTTTTTAGTAATTTGTTCATCTTTTTATTGATTATTTAATCTTCAACGGCTTTTCTAAGTTAATAATTATGAATTTCAAAGACGCAAGTGACAGAGTTTCGTCGTGTCAGCAGCTGTTTCATTGGATAATCTCGTGATTTTTTTTTTAATTTTTTCGTATAAATTTTTTTTGTTGAGTGTTCGATTCGGTATTATCTGTTTGAAGCTTTTCCAGTTCTTTGTTAGGCATAAAAACGTGTGAAATCAAGTCACAATTCGTTCATCTCTTCCGAAATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGTCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGGTGTGTTTCTTTATTACGCTTTTTTGTTTTTTGGTTTGTGATCCCTGTGGTTTTGATAAAATCTTCTGTGATTCTGTGTTGCTTTTTTCGCAAATGGTTGTGGTTTTTTCTTTAATGAGTTTGCTGTGTTTTTCGACTTTCTGCTGTTTGGTTGCTGTTTTGCTTGAGATTTTTGAGTTGTTAGCTAGTGAGAGCTTGCATGGAAATGAAAGTGAAGGAAATTTGATGAAATCTTAAGAGCTTTATTTGGATTTGATCGGACGATTCCTCCACAGATTGATGAATCTTGTGGAACTGTTAATTTCTGAGCGTGTTCTTTCTGTTCTTCATGTGATTAAATCTTGGGGCAACAGTGACCTATCAGTTTTTTGGGCTAATTAAACTTGTTAGAGTCATCCTTTCAAGCACAAAGCTAAAGAGTTCTAGGAATAAGCTACCGAAGGCTTTGTTTTGGTAAGTTTTTGGTAGGAAGAAACCAAAGATATTTCAGAGGAGATCGAGAGGTTTCAGATATTGTTCTTCATTCTAGGGCGTCCTGTTTGAGTTCTTCTATATAAACATATATCTTTTTGATGAATGGGAATTGAGCTGTTTTCTTATCACCCTTTGTTAACTGTTTAATGTTCTGGTAGCAATTTTGTTGTTTAAGAACAATATACTTCCACATTCAGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTGGCGGATCGATATGGAAAAGACCGAGTTTTCAATACCCCTCTTTGTGAGCAGGTAGCTTACTCGTCGACTTTGAGATTTTGGAGGTCGATATGAGAGTACAGCCATTGTTCTTCCTAGTAATATCATGTGATCAATATTCTATTATCGAGTATATGTGAAAAGCTTCATTTTAACTCATTTTTAATACAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGTAATTTTGAGGAATTTGCATACTTAGCCATGTGATGGAATTTCTTTGTTTCTCACATGTTTTCCCTTTTCTAACACGTAAGTCGTTTCACGTGGTAGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCGGATTACATTTATCCAGCTTTTGATCAGGTAAGCTTTCGTCTTCGTTGAAATTCTTCCTCCTGCCTTCTTGTTTGGCATTATCTTGAGAAATTTTTTGCCCTTCGTAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTAGATTTCCTCCTCTCTTTGCATACAGCCAACTTCTTTCATGTTTACTTCCTCGTTATTGACCCTTCATGTCACGCTTATTTACGAATCCTCTCAGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTCCTGGTATCAAAGTATGTTTCCGTCTTCTGCTAATTTGTCTTGATAAGTTTTAGGTATTTTCAGTACTTTGTGACAAATATGTAACAATCCAAGCTCACCGCTAGCAGATATTGTCCTCTTTGGCCCGTTACATATCGCCGTCAGCCTCATGGTTTCAAAACACGTCTACTAGGAAGAGGTTTCCACACCCTTATAAGGAATGTTTCGGTCCCTTCTCCAACCGATGTGAGATCTCACGATCCACCCCCTTGGAGGCCCAGTGTCCTCGCTGGACACTGCCCGGTGTCTGGCTATGATACCATTTGTAACAGTTCAAGCTCATAGCTTGCAGATATCATCTGCTTTAGCCCGTTATGTATCGGCGTTAGCGTTATTTATAGATTAAGGAGCCTTCATTTATGACCCATATGTTGGCAGCCAATGTATTGTCAGAGGGTTTTCTGACGAGCAAAGGATAGAGTTTTTATGATCCAAAAGTTAGCATCCCTATCTGACCGTAAAACCAGCAGTCATCTTGAAGAAGTTCAGAACTGGAGGAAAGGGGGACAGAATTTCACCTCTCTTACCTCTAGTGTGTAGACCATGCCATCTTTTCTTGCTGCCGTGTAGTCTCAATCATTTCTTGTAGAATAAACTTAACTTAGGACGTGTCTCAGTTATTTTGCTATTATATTAACTTCATTGCATGAATTATTACTTTTTTGGAAAACCATAATTTTATCGTTTTCATGCTAAACAGGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCGTGATCAAAACCCTGTTGTATTTTTTGAGCCCAAGGTATGAAAACTTTTCTAAGTTGTTTGGATCGATGTTTGTCCAGAAGTTGGGATGAAAATATTTTGAGCGTTCGTCTTCAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTTTGATCTCATCTCTTTTCATGTTCTAATTATTTATGAATAATTCCATAGTGCATTAATAACGCAATGTTTGATAAACCTGAAAACGAGATATTCAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGTAAATCTCTTTACTTATTTTCTGGTATCTTTATTGTATGACAATGGAATTTTATATGCATCTGAATGCAGGCTGAATTTCTTTCACTATTTTACTTACTGGTGTAAAGGGAAACCTTTCCATCTCGCTCTCCTTGGCTTTCAACTTATGTTCTCCTATCTGTTCATGTGTCGTTATTTTCTGCTTTTATTTAGTAATCGGGTTGGGTCTCTTTCCCTTGCATTATGTACCCTTGGCCCACTCTACTAATTTCTCGGGAGGCTTCATGTAACAGCCACAGCTCACCGCTAGCAGATATTGTCCTGTTTGGGCTTTTCCTTTTAGGCTTCCCTTCAAGGTTTTTATAACGTGTTTGCTAGGGAGAGGTTTCCATACCCCTACAAAGCGTTCTCCTCCCAACCGATGTAGGATCTCACAATTCACTCCCCTTCGGGACCCAGCGTCCTCGCTGGCACTCGTTCAGCTCCCTAATAGAAGTGGCCAAGTTCATAAATAATTAAAATGATATTACAATGGTGAAACTTAATTACTGTAGGATGAAAGCTTTTCAACATTCAATCTGAAACTTTGAAGAATATGTGTAAACTGTTTAATTTTTGACATTTCAGTCTGTGAAGTCGCTAGGATCCAAGAAAATAATCAACTAATGATCTCGATTTGCTCGATATATATTGCAGGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTGTAAGTAATATTTTCGTTTCTTTATAACAAGCTTCCTGAGAAATGAGAACTTTAAGTGAAACAGATATAGATTTATAACTAATCTCCATTTCGCTCTGTAGATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGGTGAGTGCGGATTTTCAGCATATTTCTATTGGTAAAAGTACATTTTTAAAAAGATTAGGAATCACAAACAGAGAGATGAATTTACTCCCTCGCTGATTAGAGAGGGGGCAGGTAAACTCTCCCTTCCCCTCCAACTTTCCTGAACAGACAAAGCATGGAATATACTCTCTCCTTCCCCTATGTAAATACTCCAACATTCCCTTGGTGGCCCTCCTGTTGCTTATTCCCCCTCGGTAAGTGTAACAGTCCAAGCCCATTGCTAGCAGATATTATCCTTTTTGGACTTTCCCTTTTAGGCTCCTCCTTAAGGTTTTTAAAATGCGTTTATTAGAGAGAGGTTTCCACACCCTTATAAAGAATGCTTCGTTCCCCTCTCCAACCAATGTGGGATCTGACAATTCACCCTCCTTCAAGGATCAACGTCCTCGCTGGCATTCGTTTCTCTCTCCAATCGATGTGTGATCTCATTATCCACCCCTTCAAGGCCTAGTGTCCTCGTTGGTGCACCGTCCGGTGTCCACCCCCTTTGGGGCTAGCATCCTCACTGGCACACTACTTGATGTCTGCGCTGATACCTCTGATACCATTTGTAACAGTTCAAGCCCACCACTAGCATATATTGTTTTCTTTGGGTTTTTCGTTTCAGACTTCTCCTCAAAGATTTAAAACTCGTATGCTAGGAAGAGGTTTTCGCACCTTATAAAGAAGTTTTGTTTCTCTCTTCAACCGATGTGGAATCTCACAATCCAATCCCCTTTGAGACCCAATGTCCTTGCTGGCACTCGTTCCTCTCTCTAATCGTTGTGAAATCTCACAGTAACTAACCTGTCGAGTGTGAATTTTCCTTCGTACTCGTCCTAGCGTACTGGATAGTAATGGGTGGCCTTACATCACATTTCTCTTTTTAGTAGGCTAAGACCTTTTATCTCACACCCCCAATCCAAGGAACCTTAAACTCTAGTTTGAAATTTGGAAGGTGGAGGCTTGTCCAAAGAACATGAACAATGGCTTGTAGAAAGCAACTAGTTTTACGGATATACTTCGATGTTCTTACTTTTGTTTTGAGTACTCAATACAAGTAGAGTAGATAAAATGAGTATCTCTTTCTCTTGTAGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCTTTCTACATGCCTACTAAGAACAAAGTAAGAGACTACCTTCCATCTTCTTGCAAAAGATTTGGCATAATGTTCATTCCTAACCAATATATTACCTTTCTCTCTGGCTGGTGTAGATATTGGACGCCATCAAAGCAACTGTAAAGTATTAGATCAAAAAATGGCTGCTTTCAAGCAGACAACAAGAAATGTTCTTCATTCCATCAGTCTCTATCGATGAACTAGATGATGTAGCCTTGATGATGTCCCATTGTTGGTGGCTGTAAGGGCCTTCTCATGTTGAATAACATAGTTTGAGTCAATGATGTCTGGATATCAAGATCCTTGGCAATAAAAAGTTATTATATTTATGAGAGTATATATTTACCTTTAGAAATAAATAAATGCTTCAAGAGAATG

mRNA sequence

ATTATTATTACATTTTTAATTTTTTTAGTAATTTGTTCATCTTTTTATTGATTATTTAATCTTCAACGGCTTTTCTAAGTTAATAATTATGAATTTCAAAGACGCAAGTGACAGAGTTTCGTCGTGTCAGCAGCTGTTTCATTGGATAATCTCGTGATTTTTTTTTTAATTTTTTCGTATAAATTTTTTTTGTTGAGTGTTCGATTCGGTATTATCTGTTTGAAGCTTTTCCAGTTCTTTGTTAGGCATAAAAACGTGTGAAATCAAGTCACAATTCGTTCATCTCTTCCGAAATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGTCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTGGCGGATCGATATGGAAAAGACCGAGTTTTCAATACCCCTCTTTGTGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCGGATTACATTTATCCAGCTTTTGATCAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTCCTGGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCAAGTTGGGATGAAAATATTTTGAGCGTTCGTCTTCAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCTTTCTACATGCCTACTAAGAACAAAATATTGGACGCCATCAAAGCAACTGTAAAGTATTAGATCAAAAAATGGCTGCTTTCAAGCAGACAACAAGAAATGTTCTTCATTCCATCAGTCTCTATCGATGAACTAGATGATGTAGCCTTGATGATGTCCCATTGTTGGTGGCTGTAAGGGCCTTCTCATGTTGAATAACATAGTTTGAGTCAATGATGTCTGGATATCAAGATCCTTGGCAATAAAAAGTTATTATATTTATGAGAGTATATATTTACCTTTAGAAATAAATAAATGCTTCAAGAGAATG

Coding sequence (CDS)

ATGGTATCGATTTTGAGGAGATCGCCGAAACTGTTGAACTCCATTTGTAGGAGGCGATTTTCTGCTTCCTCTGATGGAAATCTTACGAGTCATGGAGGAAAATCTATGAATCTGTATTCTGCTATCAATCAAGCGCTTCATATTGCCCTTGAAACTGATCCTCGTTCTTATGTGTTTGGAGAAGACGTGGGCTTCGGTGGGGTCTTCCGTTGCACAACTGGATTGGCGGATCGATATGGAAAAGACCGAGTTTTCAATACCCCTCTTTGTGAGCAGGGAATTGTTGGCTTTGGCATTGGTCTTGCAGCAATGGGAAATAGGGCCATAGCAGAAATTCAGTTTGCGGATTACATTTATCCAGCTTTTGATCAGATAGTTAATGAAGCTGCAAAGTTCAGATATCGTAGCGGAAATCAGTTCAATTGTGGTGGTTTAACAATTAGAGCCCCATATGGAGCTGTTGGTCATGGTGGTCATTATCACTCACAATCACCTGAAGCTTTCTTCTGTCATGTTCCTGGTTGTCATCCCTCGAAGTCCATACCAAGCCAAGGGATTGCTACTTTCATGCATCCAAGTTGGGATGAAAATATTTTGAGCGTTCGTCTTCAGTGGCTTTACCGTTTGGCTGTTGAAGAAGTTCCTGAGCATGACTTCATGTTGCCTCTATCAGAAGCAGAGGTGATCCGAGAAGGCAGTGATATTACATTGGTTGGTTGGGGAGCGCAGCTTTCTGTTATGGAACAGGCCTGTATTGATGCTGAAAAAGATGGAATCTCCTGTGAACTGATTGACCTGAGAACTCTAATACCATGGGATAAGGAAACGGTGGAGGCCTCAGTTAAGAAGACTGGGAGACTTCTTATTAGCCATGAAGCTCCGGTTACCGGAGGCTTTGGTGCTGAAATATCCGCTTCCATTGTTGAACGCTGCTTCTTACGGTTGGAGGCCCCTGTTTCCAGAGTTTGTGGATTGGACACTCCATTCCCTCTTGTGTTTGAACCTTTCTACATGCCTACTAAGAACAAAATATTGGACGCCATCAAAGCAACTGTAAAGTATTAG

Protein sequence

MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCHPSKSIPSQGIATFMHPSWDENILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Homology
BLAST of Cp4.1LG18g02820 vs. ExPASy Swiss-Prot
Match: Q9SAV3 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=BCDH BETA1 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 3.9e-161
Identity = 281/349 (80.52%), Postives = 308/349 (88.25%), Query Frame = 0

Query: 11  LLNSICRR-RFSASSDG--NLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           LL   CR+  F + S G   +++  GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLA+R+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIPSQGI 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG        P +  
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 ATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLS 250
              +    D N ++    +WLYR AVEEVPEHD+M+PLSEAEVIREG+DITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 355
           +I+ERCFL+LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIK+TV Y
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

BLAST of Cp4.1LG18g02820 vs. ExPASy Swiss-Prot
Match: Q9LDY2 (2-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=DIN4 PE=1 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.1e-158
Identity = 276/352 (78.41%), Postives = 300/352 (85.23%), Query Frame = 0

Query: 18  RRFSASSDGNLTSHG-------------GKSMNLYSAINQALHIALETDPRSYVFGEDVG 77
           RRF   S   ++ HG             GKSMNLYSAINQALHIALETDPRSYVFGEDVG
Sbjct: 7   RRFCRGSSFPVSGHGYRMLSTVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVG 66

Query: 78  FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 137
           FGGVFRCTTGLA+R+GK RVFNTPLCEQGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQ
Sbjct: 67  FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQ 126

Query: 138 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIPS 197
           IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG        P 
Sbjct: 127 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPR 186

Query: 198 QGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 257
           +     +    D N ++    +WLYR AVE+VPE D+M+PLSEAEV+REGSDITLVGWGA
Sbjct: 187 EAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVMREGSDITLVGWGA 246

Query: 258 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 317
           QL++MEQAC+DAE +GISCELIDL+TLIPWDKE VE SV+KTGRLLISHEAPVTGGFGAE
Sbjct: 247 QLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAE 306

Query: 318 ISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 355
           I+A+IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAI++TV Y
Sbjct: 307 IAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY 358

BLAST of Cp4.1LG18g02820 vs. ExPASy Swiss-Prot
Match: Q55FN7 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=bkdB PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 3.0e-116
Identity = 205/325 (63.08%), Postives = 248/325 (76.31%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQG 93
           + MNL+ AIN  + IA++ D ++ VFGEDVGFGGVFRCT GL D+YG  RVFNTPLCEQG
Sbjct: 47  QKMNLFQAINNGMDIAMQKDSKAVVFGEDVGFGGVFRCTVGLRDKYGASRVFNTPLCEQG 106

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           I GF IGLAA G   IAEIQFADYI+PAFDQIVNEAAK+RYRSG QF+CG LTIR+PYGA
Sbjct: 107 IAGFAIGLAAQGATPIAEIQFADYIFPAFDQIVNEAAKYRYRSGGQFDCGSLTIRSPYGA 166

Query: 154 VGHGGHYHSQSPEAFFCHVPGCH---PSKSIPSQGIATFMHPSWDENILSVRLQWLYRLA 213
           VGHGGHYHSQSPE++F H PG     PS  I ++G+        D  ++    + +YR A
Sbjct: 167 VGHGGHYHSQSPESYFGHTPGLKVVIPSTPIEAKGLLLASIREKDP-VIFFEPKLMYRSA 226

Query: 214 VEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVMEQAC-IDAEKDGISCELIDLRTL 273
           VEEVP  D+ +PL +A +++EG DIT++GWGAQ+ V+ QA  +  EK GISCELIDLRT+
Sbjct: 227 VEEVPIGDYEIPLGKARIVKEGKDITIIGWGAQMRVLLQAVNMAEEKLGISCELIDLRTI 286

Query: 274 IPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPF 333
            PWD ETV  SVKKTGR++ISHEAP TGG+ AEISA+I ERCFL LEAP+ RVCG DTPF
Sbjct: 287 QPWDVETVVESVKKTGRVVISHEAPKTGGWAAEISATIQERCFLHLEAPIQRVCGYDTPF 346

Query: 334 PLVFEPFYMPTKNKILDAIKATVKY 355
           PL+FE FY+P   K  ++IK T+ Y
Sbjct: 347 PLIFEKFYVPDHLKNFESIKKTMVY 370

BLAST of Cp4.1LG18g02820 vs. ExPASy Swiss-Prot
Match: Q6P3A8 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10090 GN=Bckdhb PE=1 SV=2)

HSP 1 Score: 412.5 bits (1059), Expect = 4.7e-114
Identity = 203/326 (62.27%), Postives = 247/326 (75.77%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL D+YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGCH---PSKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRL 213
           VGHG  YHSQSPEAFF H PG     P     ++G+   +    D+N  +    + LYR 
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGL--LLSCIEDKNPCIFFEPKILYRA 246

Query: 214 AVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRT 273
           AVE+VP   + +PLS+AEVI+EGSD+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT
Sbjct: 247 AVEQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRT 306

Query: 274 LIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTP 333
           ++PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAP+SRVCG DTP
Sbjct: 307 IVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTP 366

Query: 334 FPLVFEPFYMPTKNKILDAIKATVKY 355
           FP +FEPFY+P K K  DA++  + Y
Sbjct: 367 FPHIFEPFYIPDKWKCYDALRKMINY 390

BLAST of Cp4.1LG18g02820 vs. ExPASy Swiss-Prot
Match: P35738 (2-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus OX=10116 GN=Bckdhb PE=1 SV=3)

HSP 1 Score: 412.5 bits (1059), Expect = 4.7e-114
Identity = 203/326 (62.27%), Postives = 247/326 (75.77%), Query Frame = 0

Query: 34  KSMNLYSAINQALHIALETDPRSYVFGEDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQG 93
           + MNL+ +I  AL  +L  DP + +FGEDV FGGVFRCT GL D+YGKDRVFNTPLCEQG
Sbjct: 67  QKMNLFQSITSALDNSLAKDPTAVIFGEDVAFGGVFRCTVGLRDKYGKDRVFNTPLCEQG 126

Query: 94  IVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGA 153
           IVGFGIG+A  G  AIAEIQFADYI+PAFDQIVNEAAK+RYRSG+ FNCG LTIRAP+G 
Sbjct: 127 IVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRAPWGC 186

Query: 154 VGHGGHYHSQSPEAFFCHVPGCH---PSKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRL 213
           VGHG  YHSQSPEAFF H PG     P     ++G+   +    D+N  +    + LYR 
Sbjct: 187 VGHGALYHSQSPEAFFAHCPGIKVVIPRSPFQAKGL--LLSCIEDKNPCIFFEPKILYRA 246

Query: 214 AVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLSVM-EQACIDAEKDGISCELIDLRT 273
           AVE+VP   + +PLS+AEVI+EGSD+TLV WG Q+ V+ E A +  EK G+SCE+IDLRT
Sbjct: 247 AVEQVPVEPYKIPLSQAEVIQEGSDVTLVAWGTQVHVIREVASMAQEKLGVSCEVIDLRT 306

Query: 274 LIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTP 333
           ++PWD +TV  SV KTGRLLISHEAP+TGGF +EIS+++ E CFL LEAP+SRVCG DTP
Sbjct: 307 IVPWDVDTVCKSVIKTGRLLISHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTP 366

Query: 334 FPLVFEPFYMPTKNKILDAIKATVKY 355
           FP +FEPFY+P K K  DA++  + Y
Sbjct: 367 FPHIFEPFYIPDKWKCYDALRKMINY 390

BLAST of Cp4.1LG18g02820 vs. NCBI nr
Match: XP_023515905.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656 bits (1693), Expect = 8.61e-237
Identity = 328/358 (91.62%), Postives = 336/358 (93.85%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    +WLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. NCBI nr
Match: XP_022960762.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata] >KAG6590071.1 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655 bits (1689), Expect = 3.51e-236
Identity = 327/358 (91.34%), Postives = 335/358 (93.58%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    +WLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. NCBI nr
Match: XP_022987320.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 642 bits (1655), Expect = 5.32e-231
Identity = 322/358 (89.94%), Postives = 333/358 (93.02%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVSILRRSPKLL+SICRR+FSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    + LYRLAVEEVPE DFMLPLSEAEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. NCBI nr
Match: KAG7023738.1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639 bits (1649), Expect = 2.23e-229
Identity = 319/350 (91.14%), Postives = 324/350 (92.57%), Query Frame = 0

Query: 6   RRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 65
           RRSPKL NSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF
Sbjct: 55  RRSPKLSNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGF 114

Query: 66  GGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 125
           GGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI
Sbjct: 115 GGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQI 174

Query: 126 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCHPSKSIPSQG 185
           VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHV         P Q 
Sbjct: 175 VNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVV----IPRSPYQA 234

Query: 186 IATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQL 245
               +    D+N ++    +WLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQL
Sbjct: 235 KGLLLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQL 294

Query: 246 SVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEIS 305
           SVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEIS
Sbjct: 295 SVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEIS 354

Query: 306 ASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           ASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 355 ASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 400

BLAST of Cp4.1LG18g02820 vs. NCBI nr
Match: XP_008450996.1 (PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis melo])

HSP 1 Score: 632 bits (1629), Expect = 4.87e-227
Identity = 311/358 (86.87%), Postives = 330/358 (92.18%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           M S+LRRS  +LNS+CRR+FSAS DGNLT+H GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    DEN ++    +WLYRLAVEEVPE DFMLPLS+AEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQAC+DAEK+GISCELIDLRTL+PWDKETVEASV+KTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. ExPASy TrEMBL
Match: A0A6J1H8B9 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111461465 PE=4 SV=1)

HSP 1 Score: 655 bits (1689), Expect = 1.70e-236
Identity = 327/358 (91.34%), Postives = 335/358 (93.58%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    +WLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. ExPASy TrEMBL
Match: A0A6J1JGI1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484908 PE=4 SV=1)

HSP 1 Score: 642 bits (1655), Expect = 2.57e-231
Identity = 322/358 (89.94%), Postives = 333/358 (93.02%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MVSILRRSPKLL+SICRR+FSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVSILRRSPKLLSSICRRQFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGL DRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLVDRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    + LYRLAVEEVPE DFMLPLSEAEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKLLYRLAVEEVPEDDFMLPLSEAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLMPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. ExPASy TrEMBL
Match: A0A1S3BQK1 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103492412 PE=4 SV=1)

HSP 1 Score: 632 bits (1629), Expect = 2.36e-227
Identity = 311/358 (86.87%), Postives = 330/358 (92.18%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           M S+LRRS  +LNS+CRR+FSAS DGNLT+H GKSMNLYSAINQALHIALETDPR+YVFG
Sbjct: 1   MASVLRRSQTVLNSLCRRQFSASPDGNLTTHAGKSMNLYSAINQALHIALETDPRAYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    DEN ++    +WLYRLAVEEVPE DFMLPLS+AEVIREGSDIT
Sbjct: 181 RSPYQAKGL--LLSCIRDENPVIFFEPKWLYRLAVEEVPEDDFMLPLSQAEVIREGSDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQAC+DAEK+GISCELIDLRTL+PWDKETVEASV+KTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACVDAEKEGISCELIDLRTLLPWDKETVEASVRKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 356

BLAST of Cp4.1LG18g02820 vs. ExPASy TrEMBL
Match: A0A6J1D329 (2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016561 PE=4 SV=1)

HSP 1 Score: 629 bits (1622), Expect = 2.75e-226
Identity = 312/358 (87.15%), Postives = 330/358 (92.18%), Query Frame = 0

Query: 1   MVSILRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFG 60
           MV+ILRRS  +LNSICRR+FS+SSDG+LT   GKSMNLYSAINQALHIALETDPRSYVFG
Sbjct: 1   MVTILRRSQTVLNSICRRKFSSSSDGDLTKPSGKSMNLYSAINQALHIALETDPRSYVFG 60

Query: 61  EDVGFGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120
           EDVGFGGVFRCTTGLA+RYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP
Sbjct: 61  EDVGFGGVFRCTTGLAERYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYP 120

Query: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH---P 180
           AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG     P
Sbjct: 121 AFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIP 180

Query: 181 SKSIPSQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDIT 240
                ++G+   +    D+N ++    +WLYRLAVEEVPEHDFMLPLSEAEVIREG+DIT
Sbjct: 181 RSPYQAKGL--LLSCIRDQNPVVFFEPKWLYRLAVEEVPEHDFMLPLSEAEVIREGNDIT 240

Query: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVT 300
           LVGWGAQLSVMEQACIDAEKDGISCELIDLRTL+PWDKETVEASVKKTGRLLISHEAPVT
Sbjct: 241 LVGWGAQLSVMEQACIDAEKDGISCELIDLRTLLPWDKETVEASVKKTGRLLISHEAPVT 300

Query: 301 GGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           GGFGAEISASIVERCFLRLEAP+SRVCGLDTPFPLVFEP YMPTKNKILDAIK+TVKY
Sbjct: 301 GGFGAEISASIVERCFLRLEAPISRVCGLDTPFPLVFEPXYMPTKNKILDAIKSTVKY 356

BLAST of Cp4.1LG18g02820 vs. ExPASy TrEMBL
Match: A0A660KMJ8 (Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_009543 PE=4 SV=1)

HSP 1 Score: 582 bits (1500), Expect = 1.09e-207
Identity = 286/352 (81.25%), Postives = 308/352 (87.50%), Query Frame = 0

Query: 5   LRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG 64
           LR   + +  + RR FS S D  L     KS NLYSAINQALHIALETDPR+YVFGEDVG
Sbjct: 6   LRTIGRSICRVSRRGFSTSCDDKLVQPSSKSTNLYSAINQALHIALETDPRAYVFGEDVG 65

Query: 65  FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 124
           FGGVFRCTTGLADR+GK RVFNTPLCEQGI+GFGIGLAAMGNRA+AEIQFADYIYPAFDQ
Sbjct: 66  FGGVFRCTTGLADRFGKSRVFNTPLCEQGIIGFGIGLAAMGNRAVAEIQFADYIYPAFDQ 125

Query: 125 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCHPS-KSIPS 184
           IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG        P 
Sbjct: 126 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPQ 185

Query: 185 QGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 244
           Q     +    D N ++    +WLYRLAVEEVPE+D+MLPLSEAEVIREGSDITLVGWGA
Sbjct: 186 QAKGLLLSCIRDPNPVVFFEPKWLYRLAVEEVPEYDYMLPLSEAEVIREGSDITLVGWGA 245

Query: 245 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 304
           QLSVMEQAC+DAEK+GISCELIDL+TLIPWDKET+E+SV+KTGRLLISHEAPVTGGFGAE
Sbjct: 246 QLSVMEQACLDAEKEGISCELIDLKTLIPWDKETIESSVRKTGRLLISHEAPVTGGFGAE 305

Query: 305 ISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 354
           ISASIVERCFLRLEAPV+RVCGLDTPFPLVFEPFYMPTKNKILDAIK+T+ Y
Sbjct: 306 ISASIVERCFLRLEAPVARVCGLDTPFPLVFEPFYMPTKNKILDAIKSTINY 357

BLAST of Cp4.1LG18g02820 vs. TAIR 10
Match: AT1G55510.1 (branched-chain alpha-keto acid decarboxylase E1 beta subunit )

HSP 1 Score: 568.9 bits (1465), Expect = 2.8e-162
Identity = 281/349 (80.52%), Postives = 308/349 (88.25%), Query Frame = 0

Query: 11  LLNSICRR-RFSASSDG--NLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVGFGG 70
           LL   CR+  F + S G   +++  GK +NLYSAINQALHIAL+TDPRSYVFGEDVGFGG
Sbjct: 4   LLGRSCRKLSFPSLSHGARRVSTETGKPLNLYSAINQALHIALDTDPRSYVFGEDVGFGG 63

Query: 71  VFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQIVN 130
           VFRCTTGLA+R+GK+RVFNTPLCEQGIVGFGIGLAAMGNRAI EIQFADYIYPAFDQIVN
Sbjct: 64  VFRCTTGLAERFGKNRVFNTPLCEQGIVGFGIGLAAMGNRAIVEIQFADYIYPAFDQIVN 123

Query: 131 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIPSQGI 190
           EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG        P +  
Sbjct: 124 EAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPREAK 183

Query: 191 ATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGAQLS 250
              +    D N ++    +WLYR AVEEVPEHD+M+PLSEAEVIREG+DITLVGWGAQL+
Sbjct: 184 GLLLSCIRDPNPVVFFEPKWLYRQAVEEVPEHDYMIPLSEAEVIREGNDITLVGWGAQLT 243

Query: 251 VMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 310
           VMEQAC+DAEK+GISCELIDL+TL+PWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA
Sbjct: 244 VMEQACLDAEKEGISCELIDLKTLLPWDKETVEASVKKTGRLLISHEAPVTGGFGAEISA 303

Query: 311 SIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 355
           +I+ERCFL+LEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIK+TV Y
Sbjct: 304 TILERCFLKLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKSTVNY 352

BLAST of Cp4.1LG18g02820 vs. TAIR 10
Match: AT3G13450.1 (Transketolase family protein )

HSP 1 Score: 559.3 bits (1440), Expect = 2.2e-159
Identity = 276/352 (78.41%), Postives = 300/352 (85.23%), Query Frame = 0

Query: 18  RRFSASSDGNLTSHG-------------GKSMNLYSAINQALHIALETDPRSYVFGEDVG 77
           RRF   S   ++ HG             GKSMNLYSAINQALHIALETDPRSYVFGEDVG
Sbjct: 7   RRFCRGSSFPVSGHGYRMLSTVENVSESGKSMNLYSAINQALHIALETDPRSYVFGEDVG 66

Query: 78  FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 137
           FGGVFRCTTGLA+R+GK RVFNTPLCEQGIVGFGIGLAAMGNR IAEIQFADYI+PAFDQ
Sbjct: 67  FGGVFRCTTGLAERFGKSRVFNTPLCEQGIVGFGIGLAAMGNRVIAEIQFADYIFPAFDQ 126

Query: 138 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIPS 197
           IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPG        P 
Sbjct: 127 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGIKVVIPRSPR 186

Query: 198 QGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 257
           +     +    D N ++    +WLYR AVE+VPE D+M+PLSEAEV+REGSDITLVGWGA
Sbjct: 187 EAKGLLLSSIRDPNPVVFFEPKWLYRQAVEDVPEDDYMIPLSEAEVMREGSDITLVGWGA 246

Query: 258 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 317
           QL++MEQAC+DAE +GISCELIDL+TLIPWDKE VE SV+KTGRLLISHEAPVTGGFGAE
Sbjct: 247 QLTIMEQACLDAENEGISCELIDLKTLIPWDKEIVETSVRKTGRLLISHEAPVTGGFGAE 306

Query: 318 ISASIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIKATVKY 355
           I+A+IVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAI++TV Y
Sbjct: 307 IAATIVERCFLRLEAPVSRVCGLDTPFPLVFEPFYMPTKNKILDAIRSTVNY 358

BLAST of Cp4.1LG18g02820 vs. TAIR 10
Match: AT5G50850.1 (Transketolase family protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.3e-49
Identity = 123/361 (34.07%), Postives = 191/361 (52.91%), Query Frame = 0

Query: 1   MVSILR-RSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVF 60
           M+ ILR R+    +++ R RF+  S  +  + G K M +  A+N A+   +  DP+ +V 
Sbjct: 1   MLGILRQRAIDGASTLRRTRFALVSARSYAA-GAKEMTVRDALNSAIDEEMSADPKVFVM 60

Query: 61  GEDVG-FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYI 120
           GE+VG + G ++ T GL ++YG +RV++TP+ E G  G G+G A  G + + E    ++ 
Sbjct: 61  GEEVGQYQGAYKITKGLLEKYGPERVYDTPITEAGFTGIGVGAAYAGLKPVVEFMTFNFS 120

Query: 121 YPAFDQIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-- 180
             A D I+N AAK  Y S  Q N   +  R P GA    G  HSQ   A++  VPG    
Sbjct: 121 MQAIDHIINSAAKSNYMSAGQINV-PIVFRGPNGAAAGVGAQHSQCYAAWYASVPGLKVL 180

Query: 181 -PSKSIPSQGI--ATFMHPSWDENILSVRLQWLYRLAV---EEVPEHDFMLPLSEAEVIR 240
            P  +  ++G+  A    P   + ++ +  + LY  +    EE  +  F LP+ +A++ R
Sbjct: 181 APYSAEDARGLLKAAIRDP---DPVVFLENELLYGESFPISEEALDSSFCLPIGKAKIER 240

Query: 241 EGSDITLVGWGAQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLIS 300
           EG D+T+V +   +    +A     ++GIS E+I+LR++ P D+ T+ ASV+KT RL+  
Sbjct: 241 EGKDVTIVTFSKMVGFALKAAEKLAEEGISAEVINLRSIRPLDRATINASVRKTSRLVTV 300

Query: 301 HEAPVTGGFGAEISASIVERCFLRLEAPVSRVCGLDTPFPLV--FEPFYMPTKNKILDAI 350
            E     G  AEI AS+VE  F  L+APV R+ G D P P     E   +P    I+ A 
Sbjct: 301 EEGFPQHGVCAEICASVVEESFSYLDAPVERIAGADVPMPYAANLERLALPQIEDIVRAS 356

BLAST of Cp4.1LG18g02820 vs. TAIR 10
Match: AT1G30120.1 (pyruvate dehydrogenase E1 beta )

HSP 1 Score: 176.0 bits (445), Expect = 5.3e-44
Identity = 114/329 (34.65%), Postives = 168/329 (51.06%), Query Frame = 0

Query: 5   LRRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG 64
           +R S KL+ +       A+ + + ++  G  + L+ A+ + L   ++ DP   V GEDVG
Sbjct: 60  VRHSQKLIPNAV-----ATKEADTSASTGHELLLFEALQEGLEEEMDRDPHVCVMGEDVG 119

Query: 65  -FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFD 124
            +GG ++ T GLAD++G  RV +TP+CE    G GIG A  G R + E     ++  AF+
Sbjct: 120 HYGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFN 179

Query: 125 QIVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIP 184
           QI N      Y SG QF    + IR P G     G  HSQ  E++F  +PG    + S P
Sbjct: 180 QISNNCGMLHYTSGGQFTI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTP 239

Query: 185 SQGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWG 244
                        EN ++      LY L  E++P+ D++  L EAE++R G  IT++ + 
Sbjct: 240 YNAKGLMKAAIRSENPVILFEHVLLYNLK-EKIPDEDYVCNLEEAEMVRPGEHITILTYS 299

Query: 245 AQLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGA 304
                + QA       G   E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA
Sbjct: 300 RMRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLHTIGNSVKKTHRVLIVEECMRTGGIGA 359

Query: 305 EISASIVERCFLRLEAPVSRVCGLDTPFP 331
            ++A+I E     L+APV  +   D P P
Sbjct: 360 SLTAAINENFHDYLDAPVMCLSSQDVPTP 381

BLAST of Cp4.1LG18g02820 vs. TAIR 10
Match: AT2G34590.1 (Transketolase family protein )

HSP 1 Score: 175.3 bits (443), Expect = 9.0e-44
Identity = 114/328 (34.76%), Postives = 168/328 (51.22%), Query Frame = 0

Query: 6   RRSPKLLNSICRRRFSASSDGNLTSHGGKSMNLYSAINQALHIALETDPRSYVFGEDVG- 65
           R  P + N++  +  +A+S  + +S  G  + L+ A+ + L   ++ DP   V GEDVG 
Sbjct: 58  RSEPLIPNAVTTKADTAAS--STSSKPGHELLLFEALQEGLEEEMDRDPHVCVMGEDVGH 117

Query: 66  FGGVFRCTTGLADRYGKDRVFNTPLCEQGIVGFGIGLAAMGNRAIAEIQFADYIYPAFDQ 125
           +GG ++ T GLAD++G  RV +TP+CE    G GIG A  G R + E     ++  AF+Q
Sbjct: 118 YGGSYKVTKGLADKFGDLRVLDTPICENAFTGMGIGAAMTGLRPVIEGMNMGFLLLAFNQ 177

Query: 126 IVNEAAKFRYRSGNQFNCGGLTIRAPYGAVGHGGHYHSQSPEAFFCHVPGCH-PSKSIPS 185
           I N      Y SG QF    + IR P G     G  HSQ  E++F  +PG    + S P 
Sbjct: 178 ISNNCGMLHYTSGGQFTI-PVVIRGPGGVGRQLGAEHSQRLESYFQSIPGIQMVACSTPY 237

Query: 186 QGIATFMHPSWDEN-ILSVRLQWLYRLAVEEVPEHDFMLPLSEAEVIREGSDITLVGWGA 245
                       EN ++      LY L  E +P+ +++  L EAE++R G  IT++ +  
Sbjct: 238 NAKGLMKAAIRSENPVILFEHVLLYNLK-ESIPDEEYICNLEEAEMVRPGEHITILTYSR 297

Query: 246 QLSVMEQACIDAEKDGISCELIDLRTLIPWDKETVEASVKKTGRLLISHEAPVTGGFGAE 305
               + QA       G   E+ID+R+L P+D  T+  SVKKT R+LI  E   TGG GA 
Sbjct: 298 MRYHVMQAAKTLVNKGYDPEVIDIRSLKPFDLYTIGNSVKKTHRVLIVEECMRTGGIGAS 357

Query: 306 ISASIVERCFLRLEAPVSRVCGLDTPFP 331
           ++A+I E     L+APV  +   D P P
Sbjct: 358 LTAAINENFHDYLDAPVMCLSSQDVPTP 381

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAV33.9e-16180.522-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Arabidopsis thal... [more]
Q9LDY23.1e-15878.412-oxoisovalerate dehydrogenase subunit beta 2, mitochondrial OS=Arabidopsis thal... [more]
Q55FN73.0e-11663.082-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Dictyostelium disc... [more]
Q6P3A84.7e-11462.272-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Mus musculus OX=10... [more]
P357384.7e-11462.272-oxoisovalerate dehydrogenase subunit beta, mitochondrial OS=Rattus norvegicus ... [more]
Match NameE-valueIdentityDescription
XP_023515905.18.61e-23791.622-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial isoform X1 [Cucurbi... [more]
XP_022960762.13.51e-23691.342-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita moschata... [more]
XP_022987320.15.32e-23189.942-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita maxima][more]
KAG7023738.12.23e-22991.142-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucurbita argyrosp... [more]
XP_008450996.14.87e-22786.87PREDICTED: 2-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial [Cucumis... [more]
Match NameE-valueIdentityDescription
A0A6J1H8B91.70e-23691.342-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita moscha... [more]
A0A6J1JGI12.57e-23189.942-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucurbita maxima... [more]
A0A1S3BQK12.36e-22786.872-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Cucumis melo OX=... [more]
A0A6J1D3292.75e-22687.152-oxoisovalerate dehydrogenase subunit beta 1, mitochondrial OS=Momordica charan... [more]
A0A660KMJ81.09e-20781.25Transket_pyr domain-containing protein OS=Carpinus fangiana OX=176857 GN=FH972_0... [more]
Match NameE-valueIdentityDescription
AT1G55510.12.8e-16280.52branched-chain alpha-keto acid decarboxylase E1 beta subunit [more]
AT3G13450.12.2e-15978.41Transketolase family protein [more]
AT5G50850.19.3e-4934.07Transketolase family protein [more]
AT1G30120.15.3e-4434.65pyruvate dehydrogenase E1 beta [more]
AT2G34590.19.0e-4434.76Transketolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005475Transketolase-like, pyrimidine-binding domainSMARTSM00861Transket_pyr_3coord: 36..223
e-value: 3.1E-41
score: 153.0
IPR005475Transketolase-like, pyrimidine-binding domainPFAMPF02779Transket_pyrcoord: 36..175
e-value: 3.1E-31
score: 108.4
NoneNo IPR availableGENE3D3.40.50.970coord: 33..222
e-value: 1.3E-72
score: 244.9
NoneNo IPR availablePANTHERPTHR429802-OXOISOVALERATE DEHYDROGENASE SUBUNIT BETA-RELATEDcoord: 26..354
NoneNo IPR availableCDDcd07036TPP_PYR_E1-PDHc-beta_likecoord: 41..174
e-value: 1.09603E-76
score: 231.598
IPR033248Transketolase, C-terminal domainPFAMPF02780Transketolase_Ccoord: 225..344
e-value: 8.8E-36
score: 122.5
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IIGENE3D3.40.50.920coord: 223..353
e-value: 1.0E-48
score: 166.7
IPR009014Transketolase C-terminal/Pyruvate-ferredoxin oxidoreductase domain IISUPERFAMILY52922TK C-terminal domain-likecoord: 218..354
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 29..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g02820.1Cp4.1LG18g02820.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009083 branched-chain amino acid catabolic process
biological_process GO:0007584 response to nutrient
cellular_component GO:0005947 mitochondrial alpha-ketoglutarate dehydrogenase complex
molecular_function GO:0003824 catalytic activity