Cp4.1LG18g02130 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g02130
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNeutral amino acid transporter
LocationCp4.1LG18: 3768182 .. 3769609 (+)
RNA-Seq ExpressionCp4.1LG18g02130
SyntenyCp4.1LG18g02130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCATCAAATTGGTGTCCCAAATCCACACCCCCATCAAAAACCCACCTCAAATTCCGACTTACCTCCTCCAATCCTCCCCACAACCACCCCAAATTCCCCCATCCCTGCCGCAAAAAAGCTCACATTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGTGGCCCCTATGGCGAGGAGCCAGCCGTCCAAGCCGCCGGACCACTCCTCGCCATCATAGGCTTCATCGTCTTCCCTTTCATATGGAGCGTCCCAGAGGCGCTGATCACGGCTGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGCTTCGTCATCTGGGCCCAAAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATGGGCACCTGGAAAATCCTCAGCGGCGTTATCAACATAGCCGCTTACCCTGTTCTCTGCATCAATTACATTCAGAAAATCGCCCCCCAGCTCCAATCCGGCTGGCCCCGCCGCACCGCCCTCCTCGCCTCCTCCGTCGTCCTCGCCGCCCTCAACTACATCGGCCTCACCATCGTCGGATATGTCGCCGTCGTTCTAGCTCTGTTATCCATCTTACCCTTCATCTTAATGACATTAATCGCCATCCCCAAAATCAAACCCCACCGGTGGGGAAATCCCGGCGATAAATCGATAAAAACAGATTGGAATCTGTATCTCAACACTCTGTTTTGGAACCTGAATTTCTGGGATAACGTCAGCACACTCGCCGGAGAAGTAGAAAAACCCAAAAAAACCTTCCCAATTGCTCTGTTCATATCAGTAATTATCACTTGTCTTTCTTACTTAATCCCACTTCTCGCCGTCACCGGCGCCGTCGACATTCAACAATCCGCTTGGGAATCCGGATTCCACGCCCAAGCGGCAGAGACACTCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGAGCCTGCTTGTCGGCGATTGGACTATTCGAAGCTCAATTAAGTAGCAGTGCATATCAAATTCTGGGTATGGCGGAAATTGGGATTTTACCCAAATTCTTCGCGGCGAGAGCGAAATGGTTCAACACTCCATGGATTGGGATTGTGATTTGCACGGCGATCTCTGTTGGGGTTTCGTATTTGGACTTCACAGACATTGTTGCGTCGGCGAATTTCATGTATAGCTTGGGGATGCTGCTGGAATTCTCGTCGTTCATATGGCTGAGATGGAAGCATTCCGCCATGGAAAGGCCGTTCAAGGTTCCGTTGGAGCTGCCGGGGTTGATCGTGATGTGTTTGGTCCCGTCGGCGTTTTTGGTGGTTTTGATGGTTTTTACGCATACCACCGTGTTCTTGGTCAGTGCTTCCATGACCGCCGCCGGGATTGTCTGGTTCGGGTTGATGAAGATTTGTAGGAAGAAGAAGATCTTCATCTTCAATCCAAGACCTGAAGATTAG

mRNA sequence

ATGGATCATCAAATTGGTGTCCCAAATCCACACCCCCATCAAAAACCCACCTCAAATTCCGACTTACCTCCTCCAATCCTCCCCACAACCACCCCAAATTCCCCCATCCCTGCCGCAAAAAAGCTCACATTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGTGGCCCCTATGGCGAGGAGCCAGCCGTCCAAGCCGCCGGACCACTCCTCGCCATCATAGGCTTCATCGTCTTCCCTTTCATATGGAGCGTCCCAGAGGCGCTGATCACGGCTGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGCTTCGTCATCTGGGCCCAAAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATGGGCACCTGGAAAATCCTCAGCGGCGTTATCAACATAGCCGCTTACCCTGTTCTCTGCATCAATTACATTCAGAAAATCGCCCCCCAGCTCCAATCCGGCTGGCCCCGCCGCACCGCCCTCCTCGCCTCCTCCGTCGTCCTCGCCGCCCTCAACTACATCGGCCTCACCATCGTCGGATATGTCGCCGTCGTTCTAGCTCTGTTATCCATCTTACCCTTCATCTTAATGACATTAATCGCCATCCCCAAAATCAAACCCCACCGGTGGGGAAATCCCGGCGATAAATCGATAAAAACAGATTGGAATCTGTATCTCAACACTCTGTTTTGGAACCTGAATTTCTGGGATAACGTCAGCACACTCGCCGGAGAAGTAGAAAAACCCAAAAAAACCTTCCCAATTGCTCTGTTCATATCAGTAATTATCACTTGTCTTTCTTACTTAATCCCACTTCTCGCCGTCACCGGCGCCGTCGACATTCAACAATCCGCTTGGGAATCCGGATTCCACGCCCAAGCGGCAGAGACACTCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGAGCCTGCTTGTCGGCGATTGGACTATTCGAAGCTCAATTAAGTAGCAGTGCATATCAAATTCTGGGTATGGCGGAAATTGGGATTTTACCCAAATTCTTCGCGGCGAGAGCGAAATGGTTCAACACTCCATGGATTGGGATTGTGATTTGCACGGCGATCTCTGTTGGGGTTTCGTATTTGGACTTCACAGACATTGTTGCGTCGGCGAATTTCATGTATAGCTTGGGGATGCTGCTGGAATTCTCGTCGTTCATATGGCTGAGATGGAAGCATTCCGCCATGGAAAGGCCGTTCAAGGTTCCGTTGGAGCTGCCGGGGTTGATCGTGATGTGTTTGGTCCCGTCGGCGTTTTTGGTGGTTTTGATGGTTTTTACGCATACCACCGTGTTCTTGGTCAGTGCTTCCATGACCGCCGCCGGGATTGTCTGGTTCGGGTTGATGAAGATTTGTAGGAAGAAGAAGATCTTCATCTTCAATCCAAGACCTGAAGATTAG

Coding sequence (CDS)

ATGGATCATCAAATTGGTGTCCCAAATCCACACCCCCATCAAAAACCCACCTCAAATTCCGACTTACCTCCTCCAATCCTCCCCACAACCACCCCAAATTCCCCCATCCCTGCCGCAAAAAAGCTCACATTAATCCCCCTCATCTTCCTCATCTACTTCGAGGTCGCCGGTGGCCCCTATGGCGAGGAGCCAGCCGTCCAAGCCGCCGGACCACTCCTCGCCATCATAGGCTTCATCGTCTTCCCTTTCATATGGAGCGTCCCAGAGGCGCTGATCACGGCTGAGCTCTCCACCGCCTTCCCCGGCAACGGCGGCTTCGTCATCTGGGCCCAAAGAGCCTTCGGACCCTTCTGGGGCTCTCTCATGGGCACCTGGAAAATCCTCAGCGGCGTTATCAACATAGCCGCTTACCCTGTTCTCTGCATCAATTACATTCAGAAAATCGCCCCCCAGCTCCAATCCGGCTGGCCCCGCCGCACCGCCCTCCTCGCCTCCTCCGTCGTCCTCGCCGCCCTCAACTACATCGGCCTCACCATCGTCGGATATGTCGCCGTCGTTCTAGCTCTGTTATCCATCTTACCCTTCATCTTAATGACATTAATCGCCATCCCCAAAATCAAACCCCACCGGTGGGGAAATCCCGGCGATAAATCGATAAAAACAGATTGGAATCTGTATCTCAACACTCTGTTTTGGAACCTGAATTTCTGGGATAACGTCAGCACACTCGCCGGAGAAGTAGAAAAACCCAAAAAAACCTTCCCAATTGCTCTGTTCATATCAGTAATTATCACTTGTCTTTCTTACTTAATCCCACTTCTCGCCGTCACCGGCGCCGTCGACATTCAACAATCCGCTTGGGAATCCGGATTCCACGCCCAAGCGGCAGAGACACTCGCCGGAAAATGGCTCAAAATCCTCCTGGAAATCGGAGCCTGCTTGTCGGCGATTGGACTATTCGAAGCTCAATTAAGTAGCAGTGCATATCAAATTCTGGGTATGGCGGAAATTGGGATTTTACCCAAATTCTTCGCGGCGAGAGCGAAATGGTTCAACACTCCATGGATTGGGATTGTGATTTGCACGGCGATCTCTGTTGGGGTTTCGTATTTGGACTTCACAGACATTGTTGCGTCGGCGAATTTCATGTATAGCTTGGGGATGCTGCTGGAATTCTCGTCGTTCATATGGCTGAGATGGAAGCATTCCGCCATGGAAAGGCCGTTCAAGGTTCCGTTGGAGCTGCCGGGGTTGATCGTGATGTGTTTGGTCCCGTCGGCGTTTTTGGTGGTTTTGATGGTTTTTACGCATACCACCGTGTTCTTGGTCAGTGCTTCCATGACCGCCGCCGGGATTGTCTGGTTCGGGTTGATGAAGATTTGTAGGAAGAAGAAGATCTTCATCTTCAATCCAAGACCTGAAGATTAG

Protein sequence

MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Homology
BLAST of Cp4.1LG18g02130 vs. ExPASy Swiss-Prot
Match: Q9LHN7 (Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g13620 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 4.8e-178
Identity = 306/451 (67.85%), Postives = 375/451 (83.15%), Query Frame = 0

Query: 27  LPTTTPNS--PIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFI 86
           LP TT  S      AKKLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLAI+GF++FPFI
Sbjct: 13  LPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFI 72

Query: 87  WSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINY 146
           WS+PEALITAELSTAFPGNGGFVIWA RAFG F GS+MG+ K LSGVIN+A++PVLC+ Y
Sbjct: 73  WSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTY 132

Query: 147 IQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIP 206
           + K+ P L+SGWPR   + AS+VVL+ LNY GL IVGY AVVL L+S+ PF++M+ +AIP
Sbjct: 133 LDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIP 192

Query: 207 KIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVII 266
           KIKPHRWG+ G K  K DWNLY NTLFWNLNFWDNVSTLAGEV++P+KTFP+AL I+VI 
Sbjct: 193 KIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIF 252

Query: 267 TCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQL 326
           TC++YLIPL AVTGAV + QS WE+GFHA+AAE +AGKWLKI +EIGA LS+IGLFEAQL
Sbjct: 253 TCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQL 312

Query: 327 SSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMY 386
           SSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +S+G+SY++FTDI++SANF+Y
Sbjct: 313 SSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLY 372

Query: 387 SLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVS 446
           +LGM LEF+SFIWLR K   ++RP++VPL++PGL+VMCL+PSAFLV+++VF    V+L+ 
Sbjct: 373 TLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLIC 432

Query: 447 ASMTAAGIVWFGLMKICRKKKIFIFNPRPED 476
             MT   I W+ L+   RK KIF FN   +D
Sbjct: 433 GVMTIGAIGWYFLINYFRKTKIFEFNEVIDD 461

BLAST of Cp4.1LG18g02130 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 6.1e-117
Identity = 220/462 (47.62%), Postives = 311/462 (67.32%), Query Frame = 0

Query: 14  QKP---TSNSDLPPPIL--------PTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGE 73
           QKP   T N   PPP +        P T+P   +   KK+T++PL+FLI++EV+GGP+G 
Sbjct: 14  QKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGI 73

Query: 74  EPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLM 133
           E +V+AAGPLLAI+GFIVFPFIWS+PEALITAE+ T FP NGG+V+W   A GP+WG   
Sbjct: 74  EDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQ 133

Query: 134 GTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGY 193
           G  K LSGVI+ A YP+L ++Y++   P L SG PR  A+L  +V L  LNY GL+IVG 
Sbjct: 134 GWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGV 193

Query: 194 VAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVST 253
            AV+L + SILPF++M+ ++IPK+KP RW     K    +W+LYLNTLFWNLN+WD+VST
Sbjct: 194 AAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVST 253

Query: 254 LAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGK 313
           L GEVE P KT P ALF ++++   SY+ P+L  TGA+ + Q  W  G+ A   + + G 
Sbjct: 254 LTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGV 313

Query: 314 WLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICT 373
           WL   ++  A  S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TPW+GI+   
Sbjct: 314 WLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSA 373

Query: 374 AISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMC 433
           +  + +S+L F +IVA+ N +Y  GM+LEF +F+ LR K+ A  RPFK+P+ + G ++MC
Sbjct: 374 SGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMC 433

Query: 434 LVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKK 465
           + P+  + V+M FT+  V LVS +    G+V    +K   KK
Sbjct: 434 IPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKK 474

BLAST of Cp4.1LG18g02130 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.3e-111
Identity = 207/442 (46.83%), Postives = 302/442 (68.33%), Query Frame = 0

Query: 32  PNSPIPAA---KKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIWSVP 91
           P+SP  A    +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++GF++FPFIWS+P
Sbjct: 36  PSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIP 95

Query: 92  EALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYIQKI 151
           EALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGVI+ A YPVL ++Y++  
Sbjct: 96  EALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSG 155

Query: 152 APQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPKIKP 211
            P L SG PR  ++L  +++L  LNY GLTIVG+VAV++ + SILPF +M LI+IP+++P
Sbjct: 156 VPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEP 215

Query: 212 HRW-----GNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVI 271
            RW     GN        +WNLYLNTLFWNLN+WD++STLAGEVE P  T P ALF  VI
Sbjct: 216 SRWLVMDLGN-------VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVI 275

Query: 272 ITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQ 331
           +   SY+ PLLA  GA+ +++  W  G+ +  A+ L G WL+  ++  A  S +G+F A+
Sbjct: 276 LVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAE 335

Query: 332 LSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFM 391
           +SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  V +S+L F +IVA+ N +
Sbjct: 336 MSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLL 395

Query: 392 YSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLV 451
           Y +GM+LEF +F+ +R KH A  RP+K+P+   G I+MC+ P+  +  ++  +   V  V
Sbjct: 396 YCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAV 455

Query: 452 SASMTAAGIVWFGLMKICRKKK 466
           S  M   G +   L+    +K+
Sbjct: 456 SIVMMIIGFLIHPLLNHMDRKR 469

BLAST of Cp4.1LG18g02130 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.8e-111
Identity = 216/449 (48.11%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 27  LPTTTPNSPIP-AAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIW 86
           LP      P+P +A+ +++IPLIFLI++EV+GGP+G E +V AAGPLLAIIGF+V P IW
Sbjct: 67  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 126

Query: 87  SVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYI 146
           S+PEALITAEL   FP NGG+V+W   A GP+WG   G  K LSGVI+ A YPVL ++Y+
Sbjct: 127 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 186

Query: 147 QKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPK 206
           +   P L  G PR  A++  + VL  LNY GLT+VG+VA+ L + S+LPF +M LIA+PK
Sbjct: 187 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 246

Query: 207 IKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVIIT 266
           ++P RW      ++  DWNLYLNTLFWNLN+WD++STLAGEV+ P KT P ALF +VI  
Sbjct: 247 LRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFV 306

Query: 267 CLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQLS 326
            ++YL PLLA TGAV + +  W  G+ A  A+ L G WL   ++  A LS +G+F A++S
Sbjct: 307 VVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMS 366

Query: 327 SSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMYS 386
           S +YQ+LGMAE G+LP FFAAR++ + TP  GI+   +  + +S + F +IVA+ NF+Y 
Sbjct: 367 SDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYC 426

Query: 387 LGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVSA 446
            GMLLEF +FI  R +     RP++VPL   G + M + P+A + V++  +   V +VS 
Sbjct: 427 FGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSL 486

Query: 447 SMTAAGIVWFGLMKICRKKKIFIFNPRPE 475
              A G+V    ++   KK+   F+  P+
Sbjct: 487 GAVAMGLVLQPALRFVEKKRWLRFSVNPD 512

BLAST of Cp4.1LG18g02130 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 3.8e-111
Identity = 216/449 (48.11%), Postives = 300/449 (66.82%), Query Frame = 0

Query: 27  LPTTTPNSPIP-AAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIW 86
           LP      P+P +A+ +++IPLIFLI++EV+GGP+G E +V AAGPLLAIIGF+V P IW
Sbjct: 67  LPDGDAGGPMPSSARTVSMIPLIFLIFYEVSGGPFGIEDSVGAAGPLLAIIGFLVLPVIW 126

Query: 87  SVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYI 146
           S+PEALITAEL   FP NGG+V+W   A GP+WG   G  K LSGVI+ A YPVL ++Y+
Sbjct: 127 SIPEALITAELGAMFPENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYL 186

Query: 147 QKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPK 206
           +   P L  G PR  A++  + VL  LNY GLT+VG+VA+ L + S+LPF +M LIA+PK
Sbjct: 187 KSGVPALGGGAPRAFAVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPK 246

Query: 207 IKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVIIT 266
           ++P RW      ++  DWNLYLNTLFWNLN+WD++STLAGEV+ P KT P ALF +VI  
Sbjct: 247 LRPARWLVIDLHNV--DWNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFV 306

Query: 267 CLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQLS 326
            ++YL PLLA TGAV + +  W  G+ A  A+ L G WL   ++  A LS +G+F A++S
Sbjct: 307 VVAYLYPLLAGTGAVPLDRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMS 366

Query: 327 SSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMYS 386
           S +YQ+LGMAE G+LP FFAAR++ + TP  GI+   +  + +S + F +IVA+ NF+Y 
Sbjct: 367 SDSYQLLGMAERGMLPSFFAARSR-YGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYC 426

Query: 387 LGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVSA 446
            GMLLEF +FI  R +     RP++VPL   G + M + P+A + V++  +   V +VS 
Sbjct: 427 FGMLLEFVAFILHRVRRPDAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSL 486

Query: 447 SMTAAGIVWFGLMKICRKKKIFIFNPRPE 475
              A G+V    ++   KK+   F+  P+
Sbjct: 487 GAVAMGLVLQPALRFVEKKRWLRFSVNPD 512

BLAST of Cp4.1LG18g02130 vs. NCBI nr
Match: XP_023516717.1 (probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo] >XP_023516718.1 probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo] >XP_023516719.1 probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Sbjct: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475

BLAST of Cp4.1LG18g02130 vs. NCBI nr
Match: KAG7023808.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 909 bits (2349), Expect = 0.0
Identity = 463/475 (97.47%), Postives = 466/475 (98.11%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDHQI V N  PHQKPTSNSD PPPILPTTTPNSPI AAKKLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHQIRVTNTGPHQKPTSNSDPPPPILPTTTPNSPIAAAKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFGPFWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGPFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIKPHRWGN GDKSIKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNSGDKSIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGA+DIQ+SAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAIDIQKSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTH+TVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Sbjct: 421 MCLVPSAFLVVLMVFTHSTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475

BLAST of Cp4.1LG18g02130 vs. NCBI nr
Match: XP_022987472.1 (probable polyamine transporter At3g13620 [Cucurbita maxima])

HSP 1 Score: 908 bits (2347), Expect = 0.0
Identity = 462/475 (97.26%), Postives = 466/475 (98.11%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDH+I VPNPHPHQKPTSNSDLPPPILP  TPNSPI AAKKLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHRIRVPNPHPHQKPTSNSDLPPPILPRHTPNSPIAAAKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFG FWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGSFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRR+ALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRSALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIK HRWGN GDK+IKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKLHRWGNSGDKAIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSF+WLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFLWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Sbjct: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475

BLAST of Cp4.1LG18g02130 vs. NCBI nr
Match: XP_022960606.1 (probable polyamine transporter At3g13620 [Cucurbita moschata])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 462/475 (97.26%), Postives = 465/475 (97.89%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDHQI V N  PHQKPTSNSD PPPILPTTTPNSPI AA+KLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHQIRVTNTGPHQKPTSNSDSPPPILPTTTPNSPIAAAEKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFGPFWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGPFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIKPHRWGN GDKSIKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNSGDKSIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGA+DIQQSAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAIDIQQSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTH+TVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPR ED
Sbjct: 421 MCLVPSAFLVVLMVFTHSTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRLED 475

BLAST of Cp4.1LG18g02130 vs. NCBI nr
Match: KAG6590143.1 (putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 460/475 (96.84%), Postives = 464/475 (97.68%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDHQI + N   HQKPTSNSD PPPILPTTTPNS I AAKKLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHQIHLTNTGHHQKPTSNSDSPPPILPTTTPNSSIAAAKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFGPFWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGPFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIKPHRWGN GDKSIKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNSGDKSIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGA+DIQ+SAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAIDIQKSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTH+TVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Sbjct: 421 MCLVPSAFLVVLMVFTHSTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475

BLAST of Cp4.1LG18g02130 vs. ExPASy TrEMBL
Match: A0A6J1JAG3 (probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC111485011 PE=3 SV=1)

HSP 1 Score: 908 bits (2347), Expect = 0.0
Identity = 462/475 (97.26%), Postives = 466/475 (98.11%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDH+I VPNPHPHQKPTSNSDLPPPILP  TPNSPI AAKKLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHRIRVPNPHPHQKPTSNSDLPPPILPRHTPNSPIAAAKKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFG FWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGSFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRR+ALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRSALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIK HRWGN GDK+IKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKLHRWGNSGDKAIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSF+WLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFLWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED
Sbjct: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475

BLAST of Cp4.1LG18g02130 vs. ExPASy TrEMBL
Match: A0A6J1H830 (probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111461340 PE=3 SV=1)

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 462/475 (97.26%), Postives = 465/475 (97.89%), Query Frame = 0

Query: 1   MDHQIGVPNPHPHQKPTSNSDLPPPILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPY 60
           MDHQI V N  PHQKPTSNSD PPPILPTTTPNSPI AA+KLTLIPLIFLIYFEVAGGPY
Sbjct: 1   MDHQIRVTNTGPHQKPTSNSDSPPPILPTTTPNSPIAAAEKLTLIPLIFLIYFEVAGGPY 60

Query: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGS 120
           GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQ AFGPFWGS
Sbjct: 61  GEEPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQTAFGPFWGS 120

Query: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180
           LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV
Sbjct: 121 LMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIV 180

Query: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNV 240
           GYVAVVLALLSILPFILMTLIAIPKIKPHRWGN GDKSIKTDWNLYLNTLFWNLNFWDNV
Sbjct: 181 GYVAVVLALLSILPFILMTLIAIPKIKPHRWGNSGDKSIKTDWNLYLNTLFWNLNFWDNV 240

Query: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLA 300
           STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGA+DIQQSAWESGFHAQAAE LA
Sbjct: 241 STLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAIDIQQSAWESGFHAQAAEILA 300

Query: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360
           GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI
Sbjct: 301 GKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVI 360

Query: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420
           CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV
Sbjct: 361 CTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIV 420

Query: 421 MCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPED 475
           MCLVPSAFLVVLMVFTH+TVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPR ED
Sbjct: 421 MCLVPSAFLVVLMVFTHSTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRLED 475

BLAST of Cp4.1LG18g02130 vs. ExPASy TrEMBL
Match: A0A5A7UQT6 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold289G00460 PE=3 SV=1)

HSP 1 Score: 753 bits (1943), Expect = 3.09e-271
Identity = 377/463 (81.43%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 18  SNSDLPPP------ILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGP 77
           SNS LPP       ILPTTTP++P    KKLTLIPLIFLIYFEVAGGPYGEEP VQAAGP
Sbjct: 7   SNSSLPPTTDSSSQILPTTTPSTPAAVRKKLTLIPLIFLIYFEVAGGPYGEEPTVQAAGP 66

Query: 78  LLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGV 137
           LLAI+GFI+FPFIWSVPEALITAELSTAFPGNGGFVIWA+RAFGPFWGSLMGTWK+LSGV
Sbjct: 67  LLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGV 126

Query: 138 INIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLS 197
           INIAA+PVLCI+YI+KIAP L+SGWPRR A+LAS+++LA LNYIGLTIVGYVAVVLA LS
Sbjct: 127 INIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLTIVGYVAVVLAFLS 186

Query: 198 ILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPK 257
           +LPFILMT IAIPKIKPHRW   GDK    DWNLYLNTLFWNLNFWDNVSTLAGEVE P+
Sbjct: 187 LLPFILMTFIAIPKIKPHRWLILGDKE--RDWNLYLNTLFWNLNFWDNVSTLAGEVENPQ 246

Query: 258 KTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIG 317
           KTFP ALF+SVI TCLSYL+PLLAV GAVD++QSAW SGFHAQAA  +AGKWLKILLEIG
Sbjct: 247 KTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIG 306

Query: 318 ACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYL 377
           + LSAIGLFEAQLSSSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVICTAIS+ VSY+
Sbjct: 307 STLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVICTAISLAVSYM 366

Query: 378 DFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVV 437
           DFTDIVASANF+YSLGMLLE SSF+WLRWKH  ++RPFKVPL+LPGLIVMCL+P+ FLVV
Sbjct: 367 DFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGLIVMCLIPAGFLVV 426

Query: 438 LMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPE 474
           +MVFTH  V LVS  MT  GI+WFGLMKIC+KKKI  FNP PE
Sbjct: 427 VMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPE 467

BLAST of Cp4.1LG18g02130 vs. ExPASy TrEMBL
Match: A0A1S3BNB6 (probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953 PE=3 SV=1)

HSP 1 Score: 753 bits (1943), Expect = 3.09e-271
Identity = 377/463 (81.43%), Postives = 413/463 (89.20%), Query Frame = 0

Query: 18  SNSDLPPP------ILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGP 77
           SNS LPP       ILPTTTP++P    KKLTLIPLIFLIYFEVAGGPYGEEP VQAAGP
Sbjct: 7   SNSSLPPTTDSSSQILPTTTPSTPAAVRKKLTLIPLIFLIYFEVAGGPYGEEPTVQAAGP 66

Query: 78  LLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGV 137
           LLAI+GFI+FPFIWSVPEALITAELSTAFPGNGGFVIWA+RAFGPFWGSLMGTWK+LSGV
Sbjct: 67  LLAIVGFILFPFIWSVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGV 126

Query: 138 INIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLS 197
           INIAA+PVLCI+YI+KIAP L+SGWPRR A+LAS+++LA LNYIGLTIVGYVAVVLA LS
Sbjct: 127 INIAAFPVLCIDYIKKIAPPLESGWPRRIAVLASTLILALLNYIGLTIVGYVAVVLAFLS 186

Query: 198 ILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPK 257
           +LPFILMT IAIPKIKPHRW   GDK    DWNLYLNTLFWNLNFWDNVSTLAGEVE P+
Sbjct: 187 LLPFILMTFIAIPKIKPHRWLILGDKE--RDWNLYLNTLFWNLNFWDNVSTLAGEVENPQ 246

Query: 258 KTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIG 317
           KTFP ALF+SVI TCLSYL+PLLAV GAVD++QSAW SGFHAQAA  +AGKWLKILLEIG
Sbjct: 247 KTFPKALFVSVIFTCLSYLVPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKILLEIG 306

Query: 318 ACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYL 377
           + LSAIGLFEAQLSSSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVICTAIS+ VSY+
Sbjct: 307 STLSAIGLFEAQLSSSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVICTAISLAVSYM 366

Query: 378 DFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVV 437
           DFTDIVASANF+YSLGMLLE SSF+WLRWKH  ++RPFKVPL+LPGLIVMCL+P+ FLVV
Sbjct: 367 DFTDIVASANFIYSLGMLLELSSFVWLRWKHPGIKRPFKVPLKLPGLIVMCLIPAGFLVV 426

Query: 438 LMVFTHTTVFLVSASMTAAGIVWFGLMKICRKKKIFIFNPRPE 474
           +MVFTH  V LVS  MT  GI+WFGLMKIC+KKKI  FNP PE
Sbjct: 427 VMVFTHKNVLLVSVGMTVGGILWFGLMKICKKKKILEFNPEPE 467

BLAST of Cp4.1LG18g02130 vs. ExPASy TrEMBL
Match: A0A0A0M300 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1)

HSP 1 Score: 752 bits (1941), Expect = 6.96e-271
Identity = 374/449 (83.30%), Postives = 408/449 (90.87%), Query Frame = 0

Query: 26  ILPTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIW 85
           ILPTTTP++P    KKLTLIPLIFLIYFEVAGGPYGEEP VQAAGPLLAIIGFIVFPFIW
Sbjct: 22  ILPTTTPSTPPSVRKKLTLIPLIFLIYFEVAGGPYGEEPTVQAAGPLLAIIGFIVFPFIW 81

Query: 86  SVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYI 145
           SVPEALITAELSTAFPGNGGFVIWA+RAFGPFWGSLMGTWK+LSGVINIAA+PVLCI+YI
Sbjct: 82  SVPEALITAELSTAFPGNGGFVIWAERAFGPFWGSLMGTWKLLSGVINIAAFPVLCIDYI 141

Query: 146 QKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPK 205
           +KIAP L+SGWPRR A+LAS+++LAALNYIGLTIVGYVAVVLA LS+LPFILMT IAIPK
Sbjct: 142 KKIAPPLESGWPRRIAVLASTLILAALNYIGLTIVGYVAVVLAFLSLLPFILMTFIAIPK 201

Query: 206 IKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVIIT 265
           IKPHRW   GDK    DWNLYLNTLFWNLNFWDNVSTLAGEVE P+KTFP ALF+SVI T
Sbjct: 202 IKPHRWLILGDKE--RDWNLYLNTLFWNLNFWDNVSTLAGEVENPQKTFPKALFVSVIFT 261

Query: 266 CLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQLS 325
           CLSYLIPLLAV GAVD++QSAW SGFHAQAA  +AGKWLK LLEIG+ LSAIGLFEAQLS
Sbjct: 262 CLSYLIPLLAVIGAVDVEQSAWGSGFHAQAAGIIAGKWLKFLLEIGSTLSAIGLFEAQLS 321

Query: 326 SSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMYS 385
           SSAYQILGMAEIG+LPKFF +RAKWF TPWIGIVICTAIS+ VSY+DFTDIVASANF+YS
Sbjct: 322 SSAYQILGMAEIGVLPKFFGSRAKWFKTPWIGIVICTAISLAVSYMDFTDIVASANFIYS 381

Query: 386 LGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVSA 445
           LGMLLEFSSF+WLRW+H  ++RPFKVPL+LPGLI+MCL+PS FLVV+MVFTH  V LVS 
Sbjct: 382 LGMLLEFSSFVWLRWRHPGIKRPFKVPLKLPGLIMMCLIPSGFLVVVMVFTHKNVLLVSG 441

Query: 446 SMTAAGIVWFGLMKICRKKKIFIFNPRPE 474
            MT  GI+WFGLMKIC+KKKI  FNP PE
Sbjct: 442 GMTVGGILWFGLMKICKKKKILEFNPEPE 468

BLAST of Cp4.1LG18g02130 vs. TAIR 10
Match: AT3G13620.1 (Amino acid permease family protein )

HSP 1 Score: 625.5 bits (1612), Expect = 3.4e-179
Identity = 306/451 (67.85%), Postives = 375/451 (83.15%), Query Frame = 0

Query: 27  LPTTTPNS--PIPAAKKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFI 86
           LP TT  S      AKKLTLIPL+FLIYFEVAGGP+GEEPAVQAAGPLLAI+GF++FPFI
Sbjct: 13  LPVTTAESSGKKATAKKLTLIPLVFLIYFEVAGGPFGEEPAVQAAGPLLAILGFLIFPFI 72

Query: 87  WSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINY 146
           WS+PEALITAELSTAFPGNGGFVIWA RAFG F GS+MG+ K LSGVIN+A++PVLC+ Y
Sbjct: 73  WSIPEALITAELSTAFPGNGGFVIWAHRAFGSFVGSMMGSLKFLSGVINVASFPVLCVTY 132

Query: 147 IQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIP 206
           + K+ P L+SGWPR   + AS+VVL+ LNY GL IVGY AVVL L+S+ PF++M+ +AIP
Sbjct: 133 LDKLFPVLESGWPRNVCIFASTVVLSFLNYTGLAIVGYAAVVLGLVSLSPFLVMSAMAIP 192

Query: 207 KIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVII 266
           KIKPHRWG+ G K  K DWNLY NTLFWNLNFWDNVSTLAGEV++P+KTFP+AL I+VI 
Sbjct: 193 KIKPHRWGSLGTK--KKDWNLYFNTLFWNLNFWDNVSTLAGEVDEPQKTFPLALLIAVIF 252

Query: 267 TCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQL 326
           TC++YLIPL AVTGAV + QS WE+GFHA+AAE +AGKWLKI +EIGA LS+IGLFEAQL
Sbjct: 253 TCVAYLIPLFAVTGAVSVDQSRWENGFHAEAAEMIAGKWLKIWIEIGAVLSSIGLFEAQL 312

Query: 327 SSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMY 386
           SSSAYQ+ GMAE+G LPKFF  R+KWFNTPW+GI+I   +S+G+SY++FTDI++SANF+Y
Sbjct: 313 SSSAYQLEGMAELGFLPKFFGVRSKWFNTPWVGILISALMSLGLSYMNFTDIISSANFLY 372

Query: 387 SLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVS 446
           +LGM LEF+SFIWLR K   ++RP++VPL++PGL+VMCL+PSAFLV+++VF    V+L+ 
Sbjct: 373 TLGMFLEFASFIWLRRKLPQLKRPYRVPLKIPGLVVMCLIPSAFLVLILVFATKIVYLIC 432

Query: 447 ASMTAAGIVWFGLMKICRKKKIFIFNPRPED 476
             MT   I W+ L+   RK KIF FN   +D
Sbjct: 433 GVMTIGAIGWYFLINYFRKTKIFEFNEVIDD 461

BLAST of Cp4.1LG18g02130 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 422.5 bits (1085), Expect = 4.3e-118
Identity = 220/462 (47.62%), Postives = 311/462 (67.32%), Query Frame = 0

Query: 14  QKP---TSNSDLPPPIL--------PTTTPNSPIPAAKKLTLIPLIFLIYFEVAGGPYGE 73
           QKP   T N   PPP +        P T+P   +   KK+T++PL+FLI++EV+GGP+G 
Sbjct: 14  QKPRISTENPPPPPPHISIGVTTGDPATSPARTVNQIKKITVLPLVFLIFYEVSGGPFGI 73

Query: 74  EPAVQAAGPLLAIIGFIVFPFIWSVPEALITAELSTAFPGNGGFVIWAQRAFGPFWGSLM 133
           E +V+AAGPLLAI+GFIVFPFIWS+PEALITAE+ T FP NGG+V+W   A GP+WG   
Sbjct: 74  EDSVKAAGPLLAIVGFIVFPFIWSIPEALITAEMGTMFPENGGYVVWVTLAMGPYWGFQQ 133

Query: 134 GTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRRTALLASSVVLAALNYIGLTIVGY 193
           G  K LSGVI+ A YP+L ++Y++   P L SG PR  A+L  +V L  LNY GL+IVG 
Sbjct: 134 GWVKWLSGVIDNALYPILFLDYLKSGIPILGSGIPRVAAILVLTVALTYLNYRGLSIVGV 193

Query: 194 VAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSIKTDWNLYLNTLFWNLNFWDNVST 253
            AV+L + SILPF++M+ ++IPK+KP RW     K    +W+LYLNTLFWNLN+WD+VST
Sbjct: 194 AAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVSKKMKGVNWSLYLNTLFWNLNYWDSVST 253

Query: 254 LAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGK 313
           L GEVE P KT P ALF ++++   SY+ P+L  TGA+ + Q  W  G+ A   + + G 
Sbjct: 254 LTGEVENPSKTLPRALFYALLLVVFSYIFPVLTGTGAIALDQKLWTDGYFADIGKVIGGV 313

Query: 314 WLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICT 373
           WL   ++  A  S +G+F A++SS ++Q+LGMAE G+LP+ FA R++ + TPW+GI+   
Sbjct: 314 WLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMAERGMLPEVFAKRSR-YRTPWVGILFSA 373

Query: 374 AISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMC 433
           +  + +S+L F +IVA+ N +Y  GM+LEF +F+ LR K+ A  RPFK+P+ + G ++MC
Sbjct: 374 SGVIILSWLSFQEIVAAENLLYCFGMVLEFITFVRLRMKYPAASRPFKIPVGVLGSVLMC 433

Query: 434 LVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLMKICRKK 465
           + P+  + V+M FT+  V LVS +    G+V    +K   KK
Sbjct: 434 IPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQPCLKQVEKK 474

BLAST of Cp4.1LG18g02130 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 9.4e-113
Identity = 207/442 (46.83%), Postives = 302/442 (68.33%), Query Frame = 0

Query: 32  PNSPIPAA---KKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIWSVP 91
           P+SP  A    +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++GF++FPFIWS+P
Sbjct: 36  PSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIP 95

Query: 92  EALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYIQKI 151
           EALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGVI+ A YPVL ++Y++  
Sbjct: 96  EALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSG 155

Query: 152 APQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPKIKP 211
            P L SG PR  ++L  +++L  LNY GLTIVG+VAV++ + SILPF +M LI+IP+++P
Sbjct: 156 VPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEP 215

Query: 212 HRW-----GNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVI 271
            RW     GN        +WNLYLNTLFWNLN+WD++STLAGEVE P  T P ALF  VI
Sbjct: 216 SRWLVMDLGN-------VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVI 275

Query: 272 ITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQ 331
           +   SY+ PLLA  GA+ +++  W  G+ +  A+ L G WL+  ++  A  S +G+F A+
Sbjct: 276 LVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAE 335

Query: 332 LSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFM 391
           +SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  V +S+L F +IVA+ N +
Sbjct: 336 MSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLL 395

Query: 392 YSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLV 451
           Y +GM+LEF +F+ +R KH A  RP+K+P+   G I+MC+ P+  +  ++  +   V  V
Sbjct: 396 YCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAV 455

Query: 452 SASMTAAGIVWFGLMKICRKKK 466
           S  M   G +   L+    +K+
Sbjct: 456 SIVMMIIGFLIHPLLNHMDRKR 469

BLAST of Cp4.1LG18g02130 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 404.8 bits (1039), Expect = 9.4e-113
Identity = 207/442 (46.83%), Postives = 302/442 (68.33%), Query Frame = 0

Query: 32  PNSPIPAA---KKLTLIPLIFLIYFEVAGGPYGEEPAVQAAGPLLAIIGFIVFPFIWSVP 91
           P+SP  A    +K++++PL+FLI++EV+GGP+G E +V AAGPLLA++GF++FPFIWS+P
Sbjct: 20  PSSPPKATDKIRKVSMLPLVFLIFYEVSGGPFGVEDSVNAAGPLLALLGFVIFPFIWSIP 79

Query: 92  EALITAELSTAFPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYIQKI 151
           EALITAE+ T +P NGG+V+W   A GPFWG   G  K LSGVI+ A YPVL ++Y++  
Sbjct: 80  EALITAEMGTMYPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSG 139

Query: 152 APQLQSGWPRRTALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPKIKP 211
            P L SG PR  ++L  +++L  LNY GLTIVG+VAV++ + SILPF +M LI+IP+++P
Sbjct: 140 VPALGSGLPRVASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEP 199

Query: 212 HRW-----GNPGDKSIKTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVI 271
            RW     GN        +WNLYLNTLFWNLN+WD++STLAGEVE P  T P ALF  VI
Sbjct: 200 SRWLVMDLGN-------VNWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVI 259

Query: 272 ITCLSYLIPLLAVTGAVDIQQSAWESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQ 331
           +   SY+ PLLA  GA+ +++  W  G+ +  A+ L G WL+  ++  A  S +G+F A+
Sbjct: 260 LVACSYIFPLLAGIGAIPLEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAE 319

Query: 332 LSSSAYQILGMAEIGILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFM 391
           +SS ++Q+LGMAE G+LP+FFA R++ + TP +GI+   +  V +S+L F +IVA+ N +
Sbjct: 320 MSSDSFQLLGMAERGMLPEFFAKRSR-YGTPLLGILFSASGVVLLSWLSFQEIVAAENLL 379

Query: 392 YSLGMLLEFSSFIWLRWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLV 451
           Y +GM+LEF +F+ +R KH A  RP+K+P+   G I+MC+ P+  +  ++  +   V  V
Sbjct: 380 YCVGMILEFIAFVRMRMKHPAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAV 439

Query: 452 SASMTAAGIVWFGLMKICRKKK 466
           S  M   G +   L+    +K+
Sbjct: 440 SIVMMIIGFLIHPLLNHMDRKR 453

BLAST of Cp4.1LG18g02130 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 393.7 bits (1010), Expect = 2.2e-109
Identity = 205/433 (47.34%), Postives = 295/433 (68.13%), Query Frame = 0

Query: 41  KLTLIPLIFLIYFEVAGGPYGEEPAVQA-AGPLLAIIGFIVFPFIWSVPEALITAELSTA 100
           KLTL+PL+FLI++EV+GGP+G E +V++  GPLLA++GF++FP IWS+PEAL+TAEL+T+
Sbjct: 21  KLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELATS 80

Query: 101 FPGNGGFVIWAQRAFGPFWGSLMGTWKILSGVINIAAYPVLCINYIQKIAPQLQSGWPRR 160
           FP NGG+V+W   AFGPFWG   G WK  SGV++ A YPVL ++Y++   P L     R 
Sbjct: 81  FPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAARV 140

Query: 161 TALLASSVVLAALNYIGLTIVGYVAVVLALLSILPFILMTLIAIPKIKPHRWGNPGDKSI 220
            ALL  +  L  LNY GL IVG+ AVVLA+ S+ PF++M L+A+P I+P RW     +  
Sbjct: 141 PALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ-- 200

Query: 221 KTDWNLYLNTLFWNLNFWDNVSTLAGEVEKPKKTFPIALFISVIITCLSYLIPLLAVTGA 280
           K +W  Y NT+FWNLN+WD  STLAGEV++P KTFP ALF +V++   SYLIPL+A TGA
Sbjct: 201 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 260

Query: 281 VDIQQSA-WESGFHAQAAETLAGKWLKILLEIGACLSAIGLFEAQLSSSAYQILGMAEIG 340
           +    S  W  G+ A+    + G WLK  ++  A +S +GLFEA++SS A+Q+LGM+EIG
Sbjct: 261 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 320

Query: 341 ILPKFFAARAKWFNTPWIGIVICTAISVGVSYLDFTDIVASANFMYSLGMLLEFSSFIWL 400
           +LP FFA R+K + TP I I+      + +S++ F +I+   NF+Y+LGMLLEF++F+ L
Sbjct: 321 MLPAFFAQRSK-YGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKL 380

Query: 401 RWKHSAMERPFKVPLELPGLIVMCLVPSAFLVVLMVFTHTTVFLVSASMTAAGIVWFGLM 460
           R K   + RP++VPL   G+ ++CL PS  ++++MV      FL+S  +   G   +  +
Sbjct: 381 RIKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFL 440

Query: 461 KICRKKKIFIFNP 472
            + ++K+   F P
Sbjct: 441 TLVKEKQWARFIP 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHN74.8e-17867.85Probable polyamine transporter At3g13620 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9FFL16.1e-11747.62Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
Q9C6S51.3e-11146.83Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A2X8M83.8e-11148.11Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D03.8e-11148.11Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
XP_023516717.10.0100.00probable polyamine transporter At3g13620 [Cucurbita pepo subsp. pepo] >XP_023516... [more]
KAG7023808.10.097.47putative polyamine transporter, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022987472.10.097.26probable polyamine transporter At3g13620 [Cucurbita maxima][more]
XP_022960606.10.097.26probable polyamine transporter At3g13620 [Cucurbita moschata][more]
KAG6590143.10.096.84putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1JAG30.097.26probable polyamine transporter At3g13620 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1H8300.097.26probable polyamine transporter At3g13620 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A5A7UQT63.09e-27181.43Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BNB63.09e-27181.43probable polyamine transporter At3g13620 OS=Cucumis melo OX=3656 GN=LOC103491953... [more]
A0A0A0M3006.96e-27183.30Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G630310 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13620.13.4e-17967.85Amino acid permease family protein [more]
AT5G05630.14.3e-11847.62Amino acid permease family protein [more]
AT1G31830.19.4e-11346.83Amino acid permease family protein [more]
AT1G31830.29.4e-11346.83Amino acid permease family protein [more]
AT3G19553.12.2e-10947.34Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 36..475
e-value: 9.6E-61
score: 203.2
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 39..469
e-value: 8.1E-59
score: 201.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..32
NoneNo IPR availablePANTHERPTHR45826:SF8BNAC05G39680D PROTEINcoord: 25..471
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 64..454
e-value: 4.2E-38
score: 131.3
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 25..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g02130.1Cp4.1LG18g02130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity