Cp4.1LG18g01330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g01330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmetal tolerance protein 4
LocationCp4.1LG18: 2918424 .. 2922430 (-)
RNA-Seq ExpressionCp4.1LG18g01330
SyntenyCp4.1LG18g01330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAATTTGCAGTTAAAAAGGCATTTCTCCTTCTCTGTTTACCCGTGCGTTTCTCTGAGATCAGCTGCATTGGATTTGCAGCTTCGTTTCTCGATTGGCCGAGGAGGAATGCGACTCTTCCTGTGCTGAAGCTCGCCGGAAAAATGGACGGAGAACCGGATTCGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGTTCTTCTCTCTCTTCCTGATTATCGTTCTCTTATCTCTTCTGTTATTTGCTGTTTCAATTTCTTTCTGGTTGCTGAAAGAATGAAGATTATAAACGAGGAGAATTCTTGTTTTGTTTATGATTATTTGCTCTTTTTTCGCTTGTTCTTTAGTGTACTTCTGAGATGATTACGAAGGCGGAGGCAACGCTAAACTCTGTTTTATATCCTCTGTTCGTTAGAGTGAAATCTGATTAACCTAAACTGTTTTTGATGCGAAATGTTCATTTCGAATGCTCGTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTGAGGTTGTCATCTTCTGAGTAATCGCCATCTTCAATGTTCTAGGGTGGAAGAACAGATACTACTGACGTATTGATTTGTTATAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAACTATGCGAATATAGTACTTCTTTTATTGAAGGTCGTCTGTGTTACCAACCATGAATTTAGTACACTAGATGAAGAATTTCGTAGCCGTTGGCTTATAATGAGCAGCATTAATTATGATATCACCAATTTTCTGCAGACTTAATCCTAAATCAACTATTAACAAGGAAAAGAAAACGAGATAGAAAATGAATTGTTCAAATTGGTATGGCCTCAATGATGAGCATATATAAATCAAGAGCTGATTATGATTGTTTTGTGGTTTATAGGCTCTGTTCTGGTCCCTTATTCCAGACCTTGAATTCACTCTCTAGTGACAGTTTGCAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATATATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGTATCTCTTGATCTTTAAATCCAGTGTGAAATTGCATTGCTTTTCTCATTCAGCCATTTGAACCATGATCGGATAGTAGGAATATCTAGTACTTTATTGTCTATCTACCTCATATTCCTGCTAAAAATGAAGATTCTTTCTCAATTATAACAAGTTAAAGTATTGACATCCATAATTGATGGATATTACGCCTATGTCTTGTAACTAGCAGAGAGCAAGTCTGTGCAATTATCTATTCATTTTGTATCCTGGAATCTTTAATGGTTGAATCATCTTGGTCACGTCAAAATTTCATTCCAAGCATGATTGTATGATTTGGGTTCCTTCAACAATGTGGATAGCTAAACCATTTCACTTTCTTGTTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATTCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGCCCTCTGGTTTTACTGCAAAAGCTCAAGAAATGATATTGTTCGTGCTTATGCGAAGGTTGTTTAGCCTACAATTATTATTATGCAATTGTATTCAAATAAATCATTCATGGCATAAATCCTTCCAATGCTATTTCCCAGGATCATTACTTCGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCTCTAGCCATATATACGATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGGTACATATTTTCCTCGCATTTTGTTCCCTTTCTCCTAAAATGAAAATTTCTGAACAACATGCAAATTGAAAGGATGTTTGTTGGATATGCTAGTCAGTCTTCTTTTTGGGAAATCCCATGATTCCTTTTTTCTTTTTCTTCTTTTAACTTTTACTGGGTATAACAAAATTCTAAAACGACTAAGATCCGGTAAGGAGTTTTATAATAATAAGTCTAGCACCCATGGTTCTAAATGAAAATGTTCTTCATCGAGTTGTAGAGCGAAAAGAAGATATGTTGTCACAATAAATGTGATATGATTTAAAGAAGATTTGAAATAACTATATTTTTGTCATATACCTGAAGTAATTGTATGTTTGTAGCATTTGCAGTTTTGAATTGTGAGCCATTCTCTCATTTCATGATACTTGCATTCCTTCCAGCTTACAACTAACACGAGGTTAATTCTTGTTGTAGTTTCACTCGTGGGGAAATCAGCCCCCCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCAGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTCAGCCATACTTTTTCTATCTTGCATTTAGTTTTTTCGTTTATTGTATCTGCACCATAATCACCGCAATGTGATTTGCATATGATAACTTTACACTCTTAGGTACAAGCGCGAAATTGCTGACCTATTTTGAATTTGTAACTAGTGGTTATTTTGACGAGCATATTTCTAAATTCTTAAATTCACGTCTGCATGATTTTAAAACGAAGTCAAGCCCACCGCTAGCAGATATTGTCTTCTTTGAGTTTTCCTTTCGAGCTTCCTCTCAAGATTTTTAGAACACCCTCTACTAAGGGAGGTTTTCACACCCATATAAAGAATGTTTTGTTCTTCTCTCTAGCCAATGTGGGATCTCACAATCCACCCCCTCAGGATCCAGCGTCCTTGCTGGCACTCGTTCCCTTCTCCAATCGATGTGAGACTCCCCAATCCACCCTTTTTCAGGGCCCAGTGTCCTTGCTGGCACGCCGCCTTGTGTCCACCAACCTTCGGAGCTAAGCCTCCTCACTGGCACATCACCCGATGTCTGGCTCTGATACCATTTGTAACAGCCAAGCCCAACCTAGTAGAGATTGTCCTCTTTTGGCTTTTCCTTTCGGGCTTTTCCTCAAGGTTTTTAAAACGCATCTGCTAGGGCGAGGTTTCCACACCCTTATAAACAATGTTTCGTTCTCCCCCAACCGATGTGAGATCTCACACATTTCCATATTGGCACGTTCTAATAAAAAGGTTGAAACATATCTCTTAACCAACATCTATACTTGGTTGCCGTTTGATGGTTTAAACTTAACAGGAACATTGTTTCAAATACTAATGCAATTAAGAATTTGATATGCTTATACTGACAACCAATCAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACATTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAGCCCTCATGTTTTATTGATATCTATTCCATACCCATCTTGACTTGTTTTATTATGTTTCTACGTCTTGTATGTGCCTGCCTTTTCTTTTTGCCCGTTGAAAAAGGCTATATATTTTCAATCAGATCAAATAATCAGTTTCTACTTTCAAGTTTATTCTGGTCTCTTCCTTTTTTTTTTCTTTTTTTTTTTTTTGC

mRNA sequence

CAATTTGCAGTTAAAAAGGCATTTCTCCTTCTCTGTTTACCCGTGCGTTTCTCTGAGATCAGCTGCATTGGATTTGCAGCTTCGTTTCTCGATTGGCCGAGGAGGAATGCGACTCTTCCTGTGCTGAAGCTCGCCGGAAAAATGGACGGAGAACCGGATTCGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAACTATGCGAATATAGTACTTCTTTTATTGAAGACCTTGAATTCACTCTCTAGTGACAGTTTGCAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATATATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATTCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGCCCTCTGGTTTTACTGCAAAAGCTCAAGAAATGATATTGTTCGTGCTTATGCGAAGGATCATTACTTCGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCTCTAGCCATATATACGATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGTTTCACTCGTGGGGAAATCAGCCCCCCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCAGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACATTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAGCCCTCATGTTTTATTGATATCTATTCCATACCCATCTTGACTTGTTTTATTATGTTTCTACGTCTTGTATGTGCCTGCCTTTTCTTTTTGCCCGTTGAAAAAGGCTATATATTTTCAATCAGATCAAATAATCAGTTTCTACTTTCAAGTTTATTCTGGTCTCTTCCTTTTTTTTTTCTTTTTTTTTTTTTTGC

Coding sequence (CDS)

ATGGACGGAGAACCGGATTCGAATCCGAAGACGGCGTTGTTGGCAGGGGCGGTGAAGCGCGACGGCAGCGGGAGACGCGGCCGACTCAGTCGGGGATACTCGGTGAACTCGCTCCGTAGCGAGTTCATTTCTAGATTGCCTGAAAAAGTGAAGTCTCATCTTCAGGATGTTGAATCTCCCTATGAAATTGATCTTTCGCAGTCGACTGCCTTTAGCAGAGGGGAAAAGGACTACTATGAAAGGCAAATTGCTACCTTAAAGTCATTTGAGGAAGTCGATTCATTGGTGACATCTGACTGCATTGACGAGGAAGACAGTGAAGAACGAGCTCAGGAAGAGAGGGCCATGAAGATCTCAAACTATGCGAATATAGTACTTCTTTTATTGAAGACCTTGAATTCACTCTCTAGTGACAGTTTGCAGATTTATGCTACAGTACGGAGTGGATCCATAGCCATTGCTGCGTCAACACTGGATTCCTTACTTGATCTCTTGGCTGGTGGAATACTTTGGTTTACTCACTTGTACATGAAGCGAGTAAATATATATAAATTCCCGATTGGAAAGCTGAGGGTGCAGCCAGTCGGCATTATTGTATTTGCTGCTGTCATGGCTACGTTAGGATTTCAGGTATTGCTTCAAGCTGCAGAACAACTAATTCAAGATAAACCGTCTGAGGGTCTGTCGCCAGAACAATTCATCTGGTTGTGTGCAATTATGATCTCTGCCACAGTAGTAAAACTTGCCCTCTGGTTTTACTGCAAAAGCTCAAGAAATGATATTGTTCGTGCTTATGCGAAGGATCATTACTTCGATGTCGTAACAAATGTGGTTGGATTAGTTGCAGCTATTCTTGGTGATAAGATCTTTTGGTGGATAGATCCGATCGGTGCTATTGCTCTAGCCATATATACGATTTTAAATTGGTCTGGAACTGTATGGGAAAATGCAGTTTCACTCGTGGGGAAATCAGCCCCCCCTGAAGTCCTGCAAAAGTTGACTTATCTTGTCACTAGGCATCCAGAGGTCAAACGCGTCGATACAGTTCGTGCCTACACCTTTGGTGTTCTTTATTTCGTAGAGGTGGACATTGAACTTCCGGAGGAGCTGCCATTGAAAGAAGCACACGCCATTGGAGAAACATTGCAGATCAAAATCGAAAAGCTTCCAGAAGTTGAAAGGGCATTTGTTCATCTGGACTTTGAATGTGAGCATAAACCAGAGCACTCGATACTCAGAAGGTTACCAAACACTTAG

Protein sequence

MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISNYANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Homology
BLAST of Cp4.1LG18g01330 vs. ExPASy Swiss-Prot
Match: Q10PP8 (Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.3e-152
Identity = 279/390 (71.54%), Postives = 325/390 (83.33%), Query Frame = 0

Query: 31  RGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFE 90
           R  SV S+R EF+SRLP+KV   + D E P  +D S+S     GEK+YYE+Q ATL+SFE
Sbjct: 18  RRNSVGSMRGEFVSRLPKKVLDAV-DPERPSHVDFSRSKGLREGEKEYYEKQFATLRSFE 77

Query: 91  EVDSLVTSDCIDEEDS-EERAQEERAMKISNYANIVLLLLKTLNSLSSDSLQIYATVRSG 150
           EVDS+  S+ + EED   E+ Q E AMKISNYAN++LL LK           IYAT++SG
Sbjct: 78  EVDSIEESNVMSEEDDIAEQKQSEFAMKISNYANMILLALK-----------IYATIKSG 137

Query: 151 SIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGF 210
           SIAIAASTLDSLLDL+AGGILWFTHL MK +N+YK+PIGKLRVQPVGII+FAAVMATLGF
Sbjct: 138 SIAIAASTLDSLLDLMAGGILWFTHLSMKSINVYKYPIGKLRVQPVGIIIFAAVMATLGF 197

Query: 211 QVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWFYCKSSRNDIVRAYAKDH 270
           QV +QA E+LI ++  + L+P Q  WL +IMI ATVVKLALW YC++S N IVRAYAKDH
Sbjct: 198 QVFVQAVEKLIVNETPDKLTPVQLTWLYSIMIFATVVKLALWLYCRTSGNKIVRAYAKDH 257

Query: 271 YFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEV 330
           YFDVVTNVVGL AA+LGD  +WWIDP+GAIALA+YTI NWSGTVWENAVSLVG+SAPPE+
Sbjct: 258 YFDVVTNVVGLAAAVLGDMFYWWIDPVGAIALAVYTITNWSGTVWENAVSLVGESAPPEM 317

Query: 331 LQKLTYLVTR-HPEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEK 390
           LQKLTYL  R HP++KRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGE+LQIKIE+
Sbjct: 318 LQKLTYLAIRHHPQIKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGESLQIKIEE 377

Query: 391 LPEVERAFVHLDFECEHKPEHSILRRLPNT 419
           LPEVERAFVHLDFEC+HKPEH+IL +LP++
Sbjct: 378 LPEVERAFVHLDFECDHKPEHNILSKLPSS 395

BLAST of Cp4.1LG18g01330 vs. ExPASy Swiss-Prot
Match: Q9M2P2 (Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=3 SV=2)

HSP 1 Score: 518.5 bits (1334), Expect = 7.2e-146
Identity = 276/392 (70.41%), Postives = 322/392 (82.14%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDS 94
           V+S++S F + LP+K++S + D E+P  +D+S++      EK+YYERQ+ATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  -LVTSD--CID----EEDSEERAQEERAMKISNYANIVLLLLKTLNSLSSDSLQIYATVR 154
            L  SD   ID    EED  ERA +E AM+ISN+ANI LL LK           IYATV+
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALK-----------IYATVK 149

Query: 155 SGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATL 214
           SGSIAIAASTLDSLLDL+AGGILWFTHL MK VNIYK+PIGKLRVQPVGII+FAAVMATL
Sbjct: 150 SGSIAIAASTLDSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATL 209

Query: 215 GFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWFYCKSSRNDIVRAYAK 274
           GFQVLL AAEQLI ++PSE ++  Q IWL +IM+SAT +KL LW YCKSSRN IVRAYAK
Sbjct: 210 GFQVLLVAAEQLISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAK 269

Query: 275 DHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPP 334
           DH+FDVVTNV+GLVAA+L +  +WW+DP GAI LAIYTI+NWSGTV ENAVSL+G+SAPP
Sbjct: 270 DHHFDVVTNVLGLVAAVLANAFYWWLDPTGAILLAIYTIVNWSGTVMENAVSLIGQSAPP 329

Query: 335 EVLQKLTYLVTRH--PEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIK 394
           EVLQKLTYLV R     +K VDTVRAYTFGVLYFVEVDIELPE+LPLKEAHAIGE+LQIK
Sbjct: 330 EVLQKLTYLVMRQGGDNIKHVDTVRAYTFGVLYFVEVDIELPEDLPLKEAHAIGESLQIK 389

Query: 395 IEKLPEVERAFVHLDFECEHKPEHSILRRLPN 418
           +E+LPEVERAFVHLDFEC HKPEHS+L  +PN
Sbjct: 390 LEELPEVERAFVHLDFECHHKPEHSVLSTIPN 409

BLAST of Cp4.1LG18g01330 vs. ExPASy Swiss-Prot
Match: Q6Z7K5 (Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.8e-141
Identity = 270/426 (63.38%), Postives = 334/426 (78.40%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+   + +T LL G     GS R   L R  S  SLRS F+SRLP+KV+         
Sbjct: 1   MDGD---DRRTPLLGG---EGGSTRPPSLRRRDSARSLRSTFLSRLPDKVRGGGDPERPA 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLV-------TSDCIDEEDSEERAQEE 120
            ++DL+++   S+GEK+YYE+Q+ATLK FEEV++L         ++ ++ ED E++ Q E
Sbjct: 61  ADVDLTRAKGLSQGEKEYYEKQLATLKIFEEVEALCMPGEFESDAEVLELEDKEQK-QSE 120

Query: 121 RAMKISNYANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFT 180
            AMKISNYANI+LL+ K           +YAT+++GS+AIAASTLDSLLD LAGGIL+FT
Sbjct: 121 SAMKISNYANIILLVFK-----------VYATIKTGSMAIAASTLDSLLDFLAGGILYFT 180

Query: 181 HLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQF 240
           HL MK VNIYK+PIGKLRVQPVGIIVFAA+MATLGFQVL+QA EQL+++K  E ++PEQ 
Sbjct: 181 HLTMKSVNIYKYPIGKLRVQPVGIIVFAAIMATLGFQVLIQAIEQLVENKAGEKMTPEQL 240

Query: 241 IWLCAIMISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWI 300
           IWL +IM+SATVVKLAL+ YC+SS N IV+AYAKDHYFDVVTNVVGLVAA+LGDK FWWI
Sbjct: 241 IWLYSIMLSATVVKLALYIYCRSSGNSIVQAYAKDHYFDVVTNVVGLVAAVLGDKFFWWI 300

Query: 301 DPIGAIALAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRH-PEVKRVDTVRAY 360
           DP+GA+ LA+YTI+NWSGTV+ENAV+LVG+ AP ++LQKLTYL  +H P V+RVDTVRAY
Sbjct: 301 DPVGAVLLAVYTIVNWSGTVYENAVTLVGQCAPSDMLQKLTYLAMKHDPRVRRVDTVRAY 360

Query: 361 TFGVLYFVEVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL 419
           +FG LYFVEVDIEL E++ L EAH+IGE+LQ KIEKLPEVERAFVH+DFE  HKPEH + 
Sbjct: 361 SFGALYFVEVDIELSEDMRLGEAHSIGESLQDKIEKLPEVERAFVHVDFESTHKPEHRVR 408

BLAST of Cp4.1LG18g01330 vs. ExPASy Swiss-Prot
Match: Q5NA18 (Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 5.8e-95
Identity = 204/410 (49.76%), Postives = 269/410 (65.61%), Query Frame = 0

Query: 13  LLAGAVKRD--GSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTA 72
           LL  AV+    G G  G  +   +  S R  F    P +V+   Q+   P  +       
Sbjct: 16  LLLSAVEAGSFGGGGDGGGAGAAAEKSWRLNFDGFRPPEVQ---QERRPPRGLHHHCLGV 75

Query: 73  FSRGEKD----YYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQ----EERAMKISNYA 132
            S+G +D    YY++Q+  L+ F E+D+L     +     EER +    E  A+++SN A
Sbjct: 76  LSQGPEDVVAEYYQQQVEMLEGFNEMDTLTDRGFLPGMSKEEREKVARSETLAIRLSNIA 135

Query: 133 NIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNI 192
           N+VL   K           +YA+VRSGS+AI ASTLDSLLDLL+G ILWFT   M+  N 
Sbjct: 136 NMVLFAAK-----------VYASVRSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNP 195

Query: 193 YKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMIS 252
           Y++PIGK R+QP+GI+VFA+VMATLG Q++L++   L+ D     L+ EQ  W+  IM++
Sbjct: 196 YRYPIGKKRMQPLGILVFASVMATLGLQIILESVRSLLSDGDEFSLTKEQEKWVVDIMLA 255

Query: 253 ATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALA 312
            T+VKLAL  YC++  N+IV+AYA+DH+FDV+TN++GLVAA+L   I  WIDP+GAI LA
Sbjct: 256 VTLVKLALVLYCRTFTNEIVKAYAQDHFFDVITNMIGLVAALLATYIEGWIDPVGAIILA 315

Query: 313 IYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVE 372
           IYTI  WS TV EN  SLVG+SA PE LQKLTYL    H  V+ +DTVRAYTFG  YFVE
Sbjct: 316 IYTIRTWSMTVLENVHSLVGQSASPEYLQKLTYLCWNHHKAVRHIDTVRAYTFGSHYFVE 375

Query: 373 VDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSI 412
           VDI LP  +PL+EAH IGE LQ K+E+LPE+ERAFVHLD+E  H+PEH++
Sbjct: 376 VDIVLPSSMPLQEAHDIGEALQEKLERLPEIERAFVHLDYEFTHRPEHAL 411

BLAST of Cp4.1LG18g01330 vs. ExPASy Swiss-Prot
Match: O80632 (Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.7e-92
Identity = 182/339 (53.69%), Postives = 238/339 (70.21%), Query Frame = 0

Query: 77  DYYERQIATLKSFEEVDSLVTSDCIDEEDSEER----AQEERAMKISNYANIVLLLLKTL 136
           DYY++Q+  L+ F E+D L     +     EE+      E  A++ISN AN++L   K  
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAK-- 122

Query: 137 NSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRV 196
                    +YA+V SGS+AI ASTLDSLLDLL+G ILWFT   M+  N Y++PIGK R+
Sbjct: 123 ---------VYASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRM 182

Query: 197 QPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWF 256
           QP+GI+VFA+VMATLG Q++L++   ++       L+ EQ  W+  IM+S T+VKL L  
Sbjct: 183 QPLGILVFASVMATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVL 242

Query: 257 YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGT 316
           YC+S  N+IV+AYA+DH+FDV+TN++GL+A IL + I +WIDP+GAI LA+YTI  WS T
Sbjct: 243 YCRSFTNEIVKAYAQDHFFDVITNIIGLIAVILANYIDYWIDPVGAIILALYTIRTWSMT 302

Query: 317 VWENAVSLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 376
           V EN  SLVGKSA PE LQKLTYL    H  ++ +DTVRAYTFG  YFVEVDI LP ++P
Sbjct: 303 VLENVNSLVGKSARPEYLQKLTYLCWNHHKAIRHIDTVRAYTFGSHYFVEVDIVLPADMP 362

Query: 377 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHS 411
           L+ AH IGE+LQ K+E L E+ERAFVHLD+E  HKPEH+
Sbjct: 363 LQVAHDIGESLQEKLELLEEIERAFVHLDYEYTHKPEHA 390

BLAST of Cp4.1LG18g01330 vs. NCBI nr
Match: KAG7023879.1 (Metal tolerance protein 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 801 bits (2070), Expect = 4.08e-292
Identity = 415/418 (99.28%), Postives = 416/418 (99.52%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKL LW YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418

BLAST of Cp4.1LG18g01330 vs. NCBI nr
Match: XP_023516649.1 (metal tolerance protein 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 779 bits (2012), Expect = 1.86e-283
Identity = 407/418 (97.37%), Postives = 407/418 (97.37%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. NCBI nr
Match: XP_022988141.1 (metal tolerance protein 4 [Cucurbita maxima])

HSP 1 Score: 774 bits (1999), Expect = 1.78e-281
Identity = 405/418 (96.89%), Postives = 405/418 (96.89%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG PDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLV RHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVIRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. NCBI nr
Match: KAG6590238.1 (Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 774 bits (1999), Expect = 1.78e-281
Identity = 404/418 (96.65%), Postives = 405/418 (96.89%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKL LW YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. NCBI nr
Match: XP_022960581.1 (metal tolerance protein 4 [Cucurbita moschata])

HSP 1 Score: 773 bits (1996), Expect = 5.10e-281
Identity = 403/418 (96.41%), Postives = 405/418 (96.89%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQI+TLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQISTLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKL LW YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. ExPASy TrEMBL
Match: A0A6J1JGE1 (metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1)

HSP 1 Score: 774 bits (1999), Expect = 8.62e-282
Identity = 405/418 (96.89%), Postives = 405/418 (96.89%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG PDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGGPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLV RHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVIRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. ExPASy TrEMBL
Match: A0A6J1H803 (metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=1)

HSP 1 Score: 773 bits (1996), Expect = 2.47e-281
Identity = 403/418 (96.41%), Postives = 405/418 (96.89%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDGEPD+NPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP
Sbjct: 1   MDGEPDTNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLSQSTAFSRGEKDYYERQI+TLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN
Sbjct: 61  YEIDLSQSTAFSRGEKDYYERQISTLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
           ISATVVKL LW YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA
Sbjct: 241 ISATVVKLVLWLYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 407

BLAST of Cp4.1LG18g01330 vs. ExPASy TrEMBL
Match: A0A5A7THE5 (Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold824G00130 PE=4 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.46e-258
Identity = 372/418 (89.00%), Postives = 387/418 (92.58%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
             ATVVKLALW YCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIA
Sbjct: 241 TFATVVKLALWLYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL RLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSRLPNT 406

BLAST of Cp4.1LG18g01330 vs. ExPASy TrEMBL
Match: A0A1S3BQ61 (metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.46e-258
Identity = 372/418 (89.00%), Postives = 387/418 (92.58%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
             ATVVKLALW YCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIA
Sbjct: 241 TFATVVKLALWLYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRRLPNT 418
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL RLPNT
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSRLPNT 406

BLAST of Cp4.1LG18g01330 vs. ExPASy TrEMBL
Match: A0A1S3BRU5 (metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=4 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 2.47e-254
Identity = 368/414 (88.89%), Postives = 383/414 (92.51%), Query Frame = 0

Query: 1   MDGEPDSNPKTALLAGAVKRDGSGRRGRLSRGYSVNSLRSEFISRLPEKVKSHLQDVESP 60
           MDG+ D +PK  LL G+V   GSGRRGRLSR YSVNSLRSEFISRLP+K++SHLQDVESP
Sbjct: 1   MDGDSDLSPKAPLLGGSVN-GGSGRRGRLSRRYSVNSLRSEFISRLPDKLRSHLQDVESP 60

Query: 61  YEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDSLVTSDCIDEEDSEERAQEERAMKISN 120
           YEIDLS+ST FSRGEKDYYERQ+ATLKSFE+VDSLV+SDCIDEED EERAQ+ERAMKISN
Sbjct: 61  YEIDLSKSTGFSRGEKDYYERQLATLKSFEDVDSLVSSDCIDEEDMEERAQQERAMKISN 120

Query: 121 YANIVLLLLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRV 180
           YANIVLLLLK           IYATVRSGSIAIAASTLDSLLDL+AGGILWFTHLYMKRV
Sbjct: 121 YANIVLLLLK-----------IYATVRSGSIAIAASTLDSLLDLMAGGILWFTHLYMKRV 180

Query: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIM 240
           NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQA EQLIQDKPSE LS EQF+WLCAIM
Sbjct: 181 NIYKFPIGKLRVQPVGIIVFAAVMATLGFQVLLQAVEQLIQDKPSESLSSEQFVWLCAIM 240

Query: 241 ISATVVKLALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIA 300
             ATVVKLALW YCK+SRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDP+GAIA
Sbjct: 241 TFATVVKLALWLYCKNSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPVGAIA 300

Query: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPEVKRVDTVRAYTFGVLYFV 360
           LAIYTILNWSGTVWENAVSLVGKSAPPEVLQ LTYLV RHPEVKRVDTVRAYTFGVLYFV
Sbjct: 301 LAIYTILNWSGTVWENAVSLVGKSAPPEVLQMLTYLVIRHPEVKRVDTVRAYTFGVLYFV 360

Query: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILRR 414
           EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSIL R
Sbjct: 361 EVDIELPEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHSILSR 402

BLAST of Cp4.1LG18g01330 vs. TAIR 10
Match: AT3G58060.1 (Cation efflux family protein )

HSP 1 Score: 518.5 bits (1334), Expect = 5.1e-147
Identity = 276/392 (70.41%), Postives = 322/392 (82.14%), Query Frame = 0

Query: 35  VNSLRSEFISRLPEKVKSHLQDVESPYEIDLSQSTAFSRGEKDYYERQIATLKSFEEVDS 94
           V+S++S F + LP+K++S + D E+P  +D+S++      EK+YYERQ+ATLKSFEEV+S
Sbjct: 30  VSSMKSNFFADLPQKLRSKI-DPENPLHLDVSKAAGLKEDEKEYYERQLATLKSFEEVES 89

Query: 95  -LVTSD--CID----EEDSEERAQEERAMKISNYANIVLLLLKTLNSLSSDSLQIYATVR 154
            L  SD   ID    EED  ERA +E AM+ISN+ANI LL LK           IYATV+
Sbjct: 90  FLARSDEYTIDEKEEEEDRAERAAQELAMQISNWANIFLLALK-----------IYATVK 149

Query: 155 SGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRVQPVGIIVFAAVMATL 214
           SGSIAIAASTLDSLLDL+AGGILWFTHL MK VNIYK+PIGKLRVQPVGII+FAAVMATL
Sbjct: 150 SGSIAIAASTLDSLLDLMAGGILWFTHLSMKNVNIYKYPIGKLRVQPVGIIIFAAVMATL 209

Query: 215 GFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWFYCKSSRNDIVRAYAK 274
           GFQVLL AAEQLI ++PSE ++  Q IWL +IM+SAT +KL LW YCKSSRN IVRAYAK
Sbjct: 210 GFQVLLVAAEQLISNEPSEKMNHVQLIWLYSIMLSATAIKLVLWIYCKSSRNHIVRAYAK 269

Query: 275 DHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGTVWENAVSLVGKSAPP 334
           DH+FDVVTNV+GLVAA+L +  +WW+DP GAI LAIYTI+NWSGTV ENAVSL+G+SAPP
Sbjct: 270 DHHFDVVTNVLGLVAAVLANAFYWWLDPTGAILLAIYTIVNWSGTVMENAVSLIGQSAPP 329

Query: 335 EVLQKLTYLVTRH--PEVKRVDTVRAYTFGVLYFVEVDIELPEELPLKEAHAIGETLQIK 394
           EVLQKLTYLV R     +K VDTVRAYTFGVLYFVEVDIELPE+LPLKEAHAIGE+LQIK
Sbjct: 330 EVLQKLTYLVMRQGGDNIKHVDTVRAYTFGVLYFVEVDIELPEDLPLKEAHAIGESLQIK 389

Query: 395 IEKLPEVERAFVHLDFECEHKPEHSILRRLPN 418
           +E+LPEVERAFVHLDFEC HKPEHS+L  +PN
Sbjct: 390 LEELPEVERAFVHLDFECHHKPEHSVLSTIPN 409

BLAST of Cp4.1LG18g01330 vs. TAIR 10
Match: AT2G39450.1 (Cation efflux family protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-93
Identity = 182/339 (53.69%), Postives = 238/339 (70.21%), Query Frame = 0

Query: 77  DYYERQIATLKSFEEVDSLVTSDCIDEEDSEER----AQEERAMKISNYANIVLLLLKTL 136
           DYY++Q+  L+ F E+D L     +     EE+      E  A++ISN AN++L   K  
Sbjct: 63  DYYQQQVEMLEGFTEMDELAERGFVPGMSKEEQDNLAKSETLAIRISNIANMLLFAAK-- 122

Query: 137 NSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPIGKLRV 196
                    +YA+V SGS+AI ASTLDSLLDLL+G ILWFT   M+  N Y++PIGK R+
Sbjct: 123 ---------VYASVTSGSLAIIASTLDSLLDLLSGFILWFTAFSMQTPNPYQYPIGKKRM 182

Query: 197 QPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVKLALWF 256
           QP+GI+VFA+VMATLG Q++L++   ++       L+ EQ  W+  IM+S T+VKL L  
Sbjct: 183 QPLGILVFASVMATLGLQIILESLRTMLSSHKEFNLTKEQESWVVGIMLSVTLVKLLLVL 242

Query: 257 YCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTILNWSGT 316
           YC+S  N+IV+AYA+DH+FDV+TN++GL+A IL + I +WIDP+GAI LA+YTI  WS T
Sbjct: 243 YCRSFTNEIVKAYAQDHFFDVITNIIGLIAVILANYIDYWIDPVGAIILALYTIRTWSMT 302

Query: 317 VWENAVSLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIELPEELP 376
           V EN  SLVGKSA PE LQKLTYL    H  ++ +DTVRAYTFG  YFVEVDI LP ++P
Sbjct: 303 VLENVNSLVGKSARPEYLQKLTYLCWNHHKAIRHIDTVRAYTFGSHYFVEVDIVLPADMP 362

Query: 377 LKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEHS 411
           L+ AH IGE+LQ K+E L E+ERAFVHLD+E  HKPEH+
Sbjct: 363 LQVAHDIGESLQEKLELLEEIERAFVHLDYEYTHKPEHA 390

BLAST of Cp4.1LG18g01330 vs. TAIR 10
Match: AT1G79520.1 (Cation efflux family protein )

HSP 1 Score: 335.1 bits (858), Expect = 8.0e-92
Identity = 186/343 (54.23%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 68  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 127

Query: 133 LLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPI 192
           + K           +YA+V S S+A+ ASTLDSLLDLL+G ILWFT   M+  N +++PI
Sbjct: 128 VAK-----------VYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPI 187

Query: 193 GKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVK 252
           GK R+QPVGIIVFA+VMATLG QV+L++   L+    S   S E+  W+  IM SATVVK
Sbjct: 188 GKRRMQPVGIIVFASVMATLGLQVILESTRLLVSKNGSHMSSTEE-KWMIGIMASATVVK 247

Query: 253 LALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTIL 312
             L  YC+S +N+IVRAYA+DH FDV+TN VGL  A+L  K +WWIDP GAI +A+YTI 
Sbjct: 248 FLLMLYCRSFQNEIVRAYAQDHLFDVITNSVGLATAVLAVKFYWWIDPSGAILIALYTIS 307

Query: 313 NWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPE-VKRVDTVRAYTFGVLYFVEVDIEL 372
            W+ TV EN  SL+G+SAPP+ L KLT+L+  H E +K +DTVRAYTFG  YFVEVDI L
Sbjct: 308 TWARTVLENVHSLIGRSAPPDFLAKLTFLIWNHHEKIKHIDTVRAYTFGSHYFVEVDIVL 367

Query: 373 PEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEH 410
           PE++ L EAH IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 368 PEDMRLHEAHNIGETLQEKLEQLSEVERAFVHIDFEFTHRPEH 398

BLAST of Cp4.1LG18g01330 vs. TAIR 10
Match: AT1G79520.2 (Cation efflux family protein )

HSP 1 Score: 335.1 bits (858), Expect = 8.0e-92
Identity = 186/343 (54.23%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN AN+VL 
Sbjct: 80  RKVSEYYKQQEKLLEGFNEMETINETGFVSGAPTEEELKKLAKSERLAVHISNAANLVLF 139

Query: 133 LLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPI 192
           + K           +YA+V S S+A+ ASTLDSLLDLL+G ILWFT   M+  N +++PI
Sbjct: 140 VAK-----------VYASVESRSMAVIASTLDSLLDLLSGFILWFTANAMRTPNNFRYPI 199

Query: 193 GKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVK 252
           GK R+QPVGIIVFA+VMATLG QV+L++   L+    S   S E+  W+  IM SATVVK
Sbjct: 200 GKRRMQPVGIIVFASVMATLGLQVILESTRLLVSKNGSHMSSTEE-KWMIGIMASATVVK 259

Query: 253 LALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTIL 312
             L  YC+S +N+IVRAYA+DH FDV+TN VGL  A+L  K +WWIDP GAI +A+YTI 
Sbjct: 260 FLLMLYCRSFQNEIVRAYAQDHLFDVITNSVGLATAVLAVKFYWWIDPSGAILIALYTIS 319

Query: 313 NWSGTVWENAVSLVGKSAPPEVLQKLTYLVTRHPE-VKRVDTVRAYTFGVLYFVEVDIEL 372
            W+ TV EN  SL+G+SAPP+ L KLT+L+  H E +K +DTVRAYTFG  YFVEVDI L
Sbjct: 320 TWARTVLENVHSLIGRSAPPDFLAKLTFLIWNHHEKIKHIDTVRAYTFGSHYFVEVDIVL 379

Query: 373 PEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEH 410
           PE++ L EAH IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 380 PEDMRLHEAHNIGETLQEKLEQLSEVERAFVHIDFEFTHRPEH 410

BLAST of Cp4.1LG18g01330 vs. TAIR 10
Match: AT1G16310.1 (Cation efflux family protein )

HSP 1 Score: 332.4 bits (851), Expect = 5.2e-91
Identity = 180/343 (52.48%), Postives = 241/343 (70.26%), Query Frame = 0

Query: 73  RGEKDYYERQIATLKSFEEVDSL----VTSDCIDEEDSEERAQEER-AMKISNYANIVLL 132
           R   +YY++Q   L+ F E++++      S    EE+ ++ A+ ER A+ ISN  N+VL 
Sbjct: 95  RRVSEYYKKQERLLEGFNEMETIHENGFASGVPTEEEMKKLAKSERLAVHISNATNLVLF 154

Query: 133 LLKTLNSLSSDSLQIYATVRSGSIAIAASTLDSLLDLLAGGILWFTHLYMKRVNIYKFPI 192
           + K           +YA++ S S+A+ ASTLDSLLDLL+G ILWFT   M++ N + +PI
Sbjct: 155 VAK-----------VYASMESRSMAVIASTLDSLLDLLSGFILWFTANAMRKPNQFHYPI 214

Query: 193 GKLRVQPVGIIVFAAVMATLGFQVLLQAAEQLIQDKPSEGLSPEQFIWLCAIMISATVVK 252
           GK R+QPVGIIVFA+VMATLG QVLL++  QL+  K    ++  +  W+  IM+S T+VK
Sbjct: 215 GKRRMQPVGIIVFASVMATLGLQVLLESGRQLVA-KSGIHMNSTEEKWMIGIMVSVTIVK 274

Query: 253 LALWFYCKSSRNDIVRAYAKDHYFDVVTNVVGLVAAILGDKIFWWIDPIGAIALAIYTIL 312
             L  YC+  +N+IVRAYA+DH FDVVTN +GL  A+L  K +WWIDP GAI +A+YTI 
Sbjct: 275 FLLMLYCRGFQNEIVRAYAQDHLFDVVTNSIGLATAVLAVKFYWWIDPTGAILIALYTIA 334

Query: 313 NWSGTVWENAVSLVGKSAPPEVLQKLTYLV-TRHPEVKRVDTVRAYTFGVLYFVEVDIEL 372
            W+ TV EN  SL+G+SAPP+ L KLT+L+   H ++K +DTVRAYTFG  YFVEVDI L
Sbjct: 335 TWARTVLENVHSLIGRSAPPDFLAKLTFLIWNHHEQIKHIDTVRAYTFGSHYFVEVDIVL 394

Query: 373 PEELPLKEAHAIGETLQIKIEKLPEVERAFVHLDFECEHKPEH 410
           PE++ L+EAH IGETLQ K+E+L EVERAFVH+DFE  H+PEH
Sbjct: 395 PEDMRLQEAHNIGETLQEKLEQLAEVERAFVHIDFEFTHRPEH 425

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q10PP82.3e-15271.54Metal tolerance protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP4 PE=2 ... [more]
Q9M2P27.2e-14670.41Putative metal tolerance protein C3 OS=Arabidopsis thaliana OX=3702 GN=MTPC3 PE=... [more]
Q6Z7K51.8e-14163.38Metal tolerance protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP3 PE=2 ... [more]
Q5NA185.8e-9549.76Metal tolerance protein 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP5 PE=2 ... [more]
O806322.7e-9253.69Metal tolerance protein 11 OS=Arabidopsis thaliana OX=3702 GN=MTP11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAG7023879.14.08e-29299.28Metal tolerance protein 4 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023516649.11.86e-28397.37metal tolerance protein 4 [Cucurbita pepo subsp. pepo][more]
XP_022988141.11.78e-28196.89metal tolerance protein 4 [Cucurbita maxima][more]
KAG6590238.11.78e-28196.65Metal tolerance protein 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022960581.15.10e-28196.41metal tolerance protein 4 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1JGE18.62e-28296.89metal tolerance protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111485472 PE=4 SV=1[more]
A0A6J1H8032.47e-28196.41metal tolerance protein 4 OS=Cucurbita moschata OX=3662 GN=LOC111461318 PE=4 SV=... [more]
A0A5A7THE57.46e-25889.00Metal tolerance protein 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ617.46e-25889.00metal tolerance protein 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
A0A1S3BRU52.47e-25488.89metal tolerance protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492484 PE=... [more]
Match NameE-valueIdentityDescription
AT3G58060.15.1e-14770.41Cation efflux family protein [more]
AT2G39450.11.9e-9353.69Cation efflux family protein [more]
AT1G79520.18.0e-9254.23Cation efflux family protein [more]
AT1G79520.28.0e-9254.23Cation efflux family protein [more]
AT1G16310.15.2e-9152.48Cation efflux family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036837Cation efflux protein, cytoplasmic domain superfamilyGENE3D3.30.70.1350Cation efflux protein, cytoplasmic domaincoord: 323..401
e-value: 2.5E-17
score: 64.8
IPR036837Cation efflux protein, cytoplasmic domain superfamilySUPERFAMILY160240Cation efflux protein cytoplasmic domain-likecoord: 324..402
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 141..321
e-value: 2.7E-24
score: 86.0
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 140..400
e-value: 2.6E-30
score: 103.6
IPR027470Cation efflux protein, cytoplasmic domainPFAMPF16916ZT_dimercoord: 326..400
e-value: 4.2E-13
score: 49.2
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 113..320
e-value: 1.9E-34
score: 121.0
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 140..322
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR43840:SF29METAL TOLERANCE PROTEIN 3coord: 16..416
NoneNo IPR availablePANTHERPTHR43840MITOCHONDRIAL METAL TRANSPORTER 1-RELATEDcoord: 16..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g01330.1Cp4.1LG18g01330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098655 cation transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008324 cation transmembrane transporter activity