Cp4.1LG18g01080 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG18g01080
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiontranslation initiation factor eIF-2B subunit gamma-like
LocationCp4.1LG18: 2734390 .. 2739755 (+)
RNA-Seq ExpressionCp4.1LG18g01080
SyntenyCp4.1LG18g01080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACGAACAAGCCTCTGCGAAATTTTCACTAGAGAGATTTTTTCTTTTTTTTTTTTTGCTAAATTAGCGCGAAGAACAAGAAGAAGAAGAATTTCCATTCTCGAGCACAATCGATTTTGACGGAAGCTGAAGAACTCCCCGCAGATTCAGAGAGTACTTCACCTCTTCGTTTGCTTCCATGGATTTTCAGGTGGTGGTGTTAGCCGGCGGCACTTCAAAGAATCTTGTACCTCTTGTTTCCAAGGTTTCATTTCCGAACCTTATGTTCATCATCATCAACAACATTTCTCTTTCATTCATCTCAATTTATTCAGCAATTGATCAATCTTCACTTTGGAATTTGGTATTCTTCTTATGGTTTTACTCGAATGTATTTTTATTTCTCTGGTGTTTTCACCAATAGGAGCTTCCCAAGGCACTACTGCCGGTGGCGAATCGTCCGGTCCTCTCGTACGTCCTGGAGCTTTTAGAACTTAGTAACCTGAAAGATCTCATTGTTGTATGGTTCCTTTTATTACTCTAATGTATACTCTTGCATGTTCCGCTGCGGTGTATAGAGTTCATGATTTCACTGTGTTTTTATATGTGTACAGGTTGCTGAAGGTAAAGATGTGGCTCTTCGTATAGGGAGTTGGATATCAGGGGCTTATGATGATCGTCTCCGTGTAGAGGTACTGAGCTGTTTTTTAATACTTGGCTATGGAGATGGTTGAGTGTTTTGTATGATCAAATTGCTGGATGAAATATGAGGTTGCCTTTTGCCCCATTTTCCCCACGCAGGGGAGAATTAGGGCGTGCCTTTGCGCTCCCTGGACTCTGAGTAGTATAGGAGTATTCACCTGTTATCCTTTACGACGCCTTTTCGCCTGATCTTAGGTCTTGACTCACCCTTCATGGACTAACCTTGTGCAAGAACCCTTAGGCTTTGAGGACTCATGACTGAATAAACGATCATAGCGTCAATATTAGATAAAATGGTAGGACTTAGGAAAGAATGTCGAGAGTTTATTAAAATGTAAAACTGAATTTTTCACATTACATCGTTATATTCATCTTATCTTTAACCGTTTTCTCTAACATAAAAGAAAAAATGGGTGAACTTCAATGAATGGTCTTATATTACGAAAATTTTGATATTTAAATTGATGTTGATTGTTGAAGAAAAATATTAGTCGTACGAATAGAAATATTGGAAATGGAGGTTTTGGTTCATTCCACAAAATTTCTTGGGGCATTGGGTGGAAGCTCAGTGAAAGTTTGGATTGGATCAAATATTGCCTTGTTGCTGTTTGCTTGCAAGAACTTTTGTGTTTGTTCTACGTCTATTTCTCTGCTATTGACTAGTTGGAGTGATTTCTTGCAAAATTTTATAGCCTCTAGGTTGGGCTTGGACGTCTAGTTCTCCTCCCTTTTTGTACGCTCTTTTGATGAACATGAAACACGAAACACAAATGTTTTCTTTATGCCCATCAGGAAACAATTCATATTGCAATAGTATGAGAAAAGCCTTGTTGTCTGGAGTAGGGAAACTATAAATTTTAACATGGTTAAATGGTGTAATGCAAGGGTATTTCTTTTAATGTATTTAAAATAGCCATGTGTTAAGACTTTGTAAGAACTTGTGAATGGGGTAGCTTCTGTATTTATAGTTAAACGTGAATTTTTTTAATGCAGAAAATGTATTACAATAACAAATGTTAATTTTGTTGGTAACCACAATGGATTCGCTTGGTGTACAATGACTTTGAGGGAATGAGTTCAAATTCCATGGCAGTCACTTACTTAGGATGAGTCTTCTTGGTAAGCAAATGTTGCAAGGAGAGTTGGTTGTCCAATGAGGTTAATCATGTTCATTTTATATATCGAAAATGTCCAATTGGTATGTTGCAAAAGTGTGGAAGAAGTATGCAGTAAGTTTATGTTGCTTTCCTAGTTATTGAAATTTGAAATACATTTGATTTTTTTTGATGTTCGATGGACTCTGCATAATTTCATGTTTTTGATGTCGATGCTGCTGCTCTACATCACCTTCCGTTTTTACTTTTCCTTATGAATGAGAAAATCGAGAGGGTTGGTTATACACAAGTAACCTAAAAAAGCTGCTGATCTCCTCAATATATATTCTTCGAGTCTGCAATGGTTTCACAAAATTTTCCGTCAGAACGAGATTTCATAACATACACAAGCCCCATTACTACCTGCAAGGAAAAATACTGCTTGTAGTTCTTCTAAAAATTTGATATTTGTAGGTTACTTCTGTTCCTGAGGATGTTGGAACTGCTGGGGCACTTCGGGCCATTGCTCGCCACTTAACAGCAAATGATATATTGGTTAGACTTGAGCACTTTAAGCAGCATATGATCTATAACTCTATGTTTATTTACAGTTACATGTCCTTTGGTTGCAGGTTGTGAGTGGTGATCTTGTTTCTGATGTTTCTCCTGGTGCCGTTGCTGCTGCGCATAGGCGGCATGATGCTGTAGTTACTGCAATGCTCTGCTCTGTTCCAGTCAGTGGGTCTTCCGAGTCTGGATCCACTGGTGGAAAAGATAAGACCAAGAAAGCAGTAGTTCACAATGTTGTTGGACTTGATCCTACCAAGCAGTTCTTACTATACATAGCAACCGGTTAGCATTTTTTGGAATATCTCCGTAGGATTTTATTCAATATGTTCCCTTTATGTTCTTTTTATTTCAATCCAGGTGCTGAAATCGAGAAAGACTATAAAATTCAGAAGAGCATACTCCGTGCAGTTGGCCAGGTAACGGTTACTATAACAAGGTATTCTTTGGTAAAAAGCTTCCGTACTTCTAAAACCTAGTTGTCTTTCTACTGATTACTAATGTGCTTGATTGAAGTTATGGCACCAAGCCACATTAGGCCCTTTGAGGTTTCACAGTTTTGTGAAACGCTTCATTTTCGAACCTATCAATTATTTCATCTTGTTGAATTACTTTTCCTTTTTTATGATTTGAGTTTGATTATTACATTTTATGAATCCAAGTCCTAAATGGATGATGTGACTTATACATGTTTCGTGCGTTTAATGTTACTTTATCTCTTTCTTTTATGCAGATGGATATCCGAACTGATCTCATGGATGCATATATGTATGCGTTCAAAAGGTCTCAATTTAATTATCCTCCTTGTTTACTACTACAACATTAGTCATAATGATGTCTATATCCTTATGCATGTTCCATTGTATTATCTCCCAGATCTTCTTTGCAGGAGGTTCTAGATCAAAAAGATACTTTCCGAAGCTTAAGGCAAGACGTGTTGCCCTATCTTGTGCGGAGCCAGTTGGTTAGTTTGTAATGCCGTGACATATTCTATTGTGTCAACAAGTGCATATAAATTTACTGAACTAGATTAATTTCTTCAATAAATGTAGAGGTCAGAAGTTGTATTGAATCGAACACCCCAAGCGGAAGAAAATGGGAATGAGAAAGTTTCCTCCCATAAGATCCAAGTATTGATTTCACAGATACTTTCTAATTCGTCTACTACAAGTTTTCATGATCTTTATGCATATGGTGCTGATGGATCTATTCCCGTTCGAAAAACACATAAATGTTGTGTTTATATTCCCCCTAGTAGCAAGTATTGTGCTCGCTTATTTTCAATTCAAGCATACAGCGACATTAATCGGGATGTAAGTTTATTGTAGTGCTCGAACCCTCTCCTTACCTTCTTGTGTTGCATCTGTTCTCTGGACTTCATTATGTTTCTTTGACTAACGTTTGATTTAATTTTTGTTAGGTTATTGGAGAAGCAAGCCATCTTTCAGGTTACTCTTTCTCTGCTCAAAATAACATCATTCATCCTTCTGCCCAACTCGGATCAAAAACCACCGTGAGTTTATAAATACCTTCATTTTTAGTATATAATTCCAAATGTTGATACGTGCGGCATATATCGTATAGTAAAGGTCGCCAATGTAGTTTCCTTGTTCTTGTTTTCTCTTAAAAAGAAAAAATTTCTTGAGAACATGAAAGTTAAACAACAGCATAAAATGACGGTCTAGTAACATGAATCCAAACAATACCTAAAATAAACAAACAAGAAAGGGTTTCAATCCAAAAGAATAAAGTCTAATGGATACTTACAAAAATTCTTAGATAGAGGTGTTCAAAGACAGACGAGAAACATAGGAAGAGACCAAACTTCTTTTCAAAACCTCTAAAAAGCCTCGAGGAACTGGATTAGAAAAAAGAGGGATTCAGTTGTAAGATATCTAATGAAACTTGAGATCTTATTAAAAAAGAAAGATATCAAATATCTGGAGTTTCTTTTTGTATATTATATGGATGATCAGATGTACAAGGACCATGATTGGGAATTGTTTGATCGTCTTTCTCCGAGGAAGAGGCAAAACATAAGTCCTCTTTCATTCTTCTCCATGTAAGCTTGGTTTTTATTCCAGAAAGAAAGAGAAAAAAGGAAAGAAAAATATAGAGAGAATTTCCTTGTTATTCCTGAATTAATCAAATATCTGTACTTCCCGAATGATGGTTTACTTTATACTTGTGTTTATTATATTGGTCAGTCCCCTTGTACTAAGTGGGATGAGGGTTTCAGGTAGGACCCCATTGTATGTTGGGAGAAGGTTCACAGATGGGTGACAAATGTAGCGTGAAACGGTCAGTAATTGGCCAACATTGCCGGATAGGTTCCAATGTGAAGGTATCATATGATCACCTATTAATTTGGGATAAAATGAGGAATTTCTATCGTCTATTTGTTGTAAGAACCTTGTTAACTTTAACTTTTACGACCCTCGTCAATACAGATCGCTAATTCAGTCGTCATGGATCATGTTACCATTGGAGACGGTTGTTCAATTCAAGGTTCGGTTATCTGCAGCAATGTGCAACTTCAGGAGCGCGCTGTTTTAAGAGACTGTCAAGTAAGTATTTACAATTATACACACGCACTCCCCCACCCCCTCCCCCGTTCCTCTCGTGTCACCTCCTTACGTTAAACTTTCGATCCAGGTAGGAGCAGGTTTTGTGGTGACTGCTGCCTGTGAGTACAAAGGAGAGGCGTTGGCTAGAAAAGAAAAGTGAAGTTGGGGAGAGACATTGTATGAGGTATGTAGACGAAAGTGTTGGAAGGAAGCAGTGATTGAAATCATATAATCATAATGTTTCTTCCTTCTGGCTTTTTTTTGGCATCATTTCATTTGTAGTTCATATTTTGTTCACATTAGACAACATTTACTTAGTTTAATTATCTAGAGATTTACCAGACACAAATGGTGAGAGTTGCAAAAATTCTGTCATTTTTCCACTGAAATGCAATCTTATTAGAGATCGTGACTATATCGTGACTATTTAGTTCAATGATAATTGGTATATATTTAC

mRNA sequence

CGACGAACAAGCCTCTGCGAAATTTTCACTAGAGAGATTTTTTCTTTTTTTTTTTTTGCTAAATTAGCGCGAAGAACAAGAAGAAGAAGAATTTCCATTCTCGAGCACAATCGATTTTGACGGAAGCTGAAGAACTCCCCGCAGATTCAGAGAGTACTTCACCTCTTCGTTTGCTTCCATGGATTTTCAGGTGGTGGTGTTAGCCGGCGGCACTTCAAAGAATCTTGTACCTCTTGTTTCCAAGGAGCTTCCCAAGGCACTACTGCCGGTGGCGAATCGTCCGGTCCTCTCGTACGTCCTGGAGCTTTTAGAACTTAGTAACCTGAAAGATCTCATTGTTGTTGCTGAAGGTAAAGATGTGGCTCTTCGTATAGGGAGTTGGATATCAGGGGCTTATGATGATCGTCTCCGTGTAGAGGTTACTTCTGTTCCTGAGGATGTTGGAACTGCTGGGGCACTTCGGGCCATTGCTCGCCACTTAACAGCAAATGATATATTGGTTGTGAGTGGTGATCTTGTTTCTGATGTTTCTCCTGGTGCCGTTGCTGCTGCGCATAGGCGGCATGATGCTGTAGTTACTGCAATGCTCTGCTCTGTTCCAGTCAGTGGGTCTTCCGAGTCTGGATCCACTGGTGGAAAAGATAAGACCAAGAAAGCAGTAGTTCACAATGTTGTTGGACTTGATCCTACCAAGCAGTTCTTACTATACATAGCAACCGGTGCTGAAATCGAGAAAGACTATAAAATTCAGAAGAGCATACTCCGTGCAGTTGGCCAGATGGATATCCGAACTGATCTCATGGATGCATATATGTATGCGTTCAAAAGATCTTCTTTGCAGGAGGTTCTAGATCAAAAAGATACTTTCCGAAGCTTAAGGCAAGACGTGTTGCCCTATCTTGTGCGGAGCCAGTTGAGGTCAGAAGTTGTATTGAATCGAACACCCCAAGCGGAAGAAAATGGGAATGAGAAAGTTTCCTCCCATAAGATCCAAGTATTGATTTCACAGATACTTTCTAATTCGTCTACTACAAGTTTTCATGATCTTTATGCATATGGTGCTGATGGATCTATTCCCGTTCGAAAAACACATAAATGTTGTGTTTATATTCCCCCTAGTAGCAAGTATTGTGCTCGCTTATTTTCAATTCAAGCATACAGCGACATTAATCGGGATGTTATTGGAGAAGCAAGCCATCTTTCAGGTTACTCTTTCTCTGCTCAAAATAACATCATTCATCCTTCTGCCCAACTCGGATCAAAAACCACCGTAGGACCCCATTGTATGTTGGGAGAAGGTTCACAGATGGGTGACAAATGTAGCGTGAAACGGTCAGTAATTGGCCAACATTGCCGGATAGGTTCCAATGTGAAGATCGCTAATTCAGTCGTCATGGATCATGTTACCATTGGAGACGGTTGTTCAATTCAAGGTTCGGTTATCTGCAGCAATGTGCAACTTCAGGAGCGCGCTGTTTTAAGAGACTGTCAAGTAGGAGCAGGTTTTGTGGTGACTGCTGCCTGTGAGTACAAAGGAGAGGCGTTGGCTAGAAAAGAAAAGTGAAGTTGGGGAGAGACATTGTATGAGGTATGTAGACGAAAGTGTTGGAAGGAAGCAGTGATTGAAATCATATAATCATAATGTTTCTTCCTTCTGGCTTTTTTTTGGCATCATTTCATTTGTAGTTCATATTTTGTTCACATTAGACAACATTTACTTAGTTTAATTATCTAGAGATTTACCAGACACAAATGGTGAGAGTTGCAAAAATTCTGTCATTTTTCCACTGAAATGCAATCTTATTAGAGATCGTGACTATATCGTGACTATTTAGTTCAATGATAATTGGTATATATTTAC

Coding sequence (CDS)

ATGGATTTTCAGGTGGTGGTGTTAGCCGGCGGCACTTCAAAGAATCTTGTACCTCTTGTTTCCAAGGAGCTTCCCAAGGCACTACTGCCGGTGGCGAATCGTCCGGTCCTCTCGTACGTCCTGGAGCTTTTAGAACTTAGTAACCTGAAAGATCTCATTGTTGTTGCTGAAGGTAAAGATGTGGCTCTTCGTATAGGGAGTTGGATATCAGGGGCTTATGATGATCGTCTCCGTGTAGAGGTTACTTCTGTTCCTGAGGATGTTGGAACTGCTGGGGCACTTCGGGCCATTGCTCGCCACTTAACAGCAAATGATATATTGGTTGTGAGTGGTGATCTTGTTTCTGATGTTTCTCCTGGTGCCGTTGCTGCTGCGCATAGGCGGCATGATGCTGTAGTTACTGCAATGCTCTGCTCTGTTCCAGTCAGTGGGTCTTCCGAGTCTGGATCCACTGGTGGAAAAGATAAGACCAAGAAAGCAGTAGTTCACAATGTTGTTGGACTTGATCCTACCAAGCAGTTCTTACTATACATAGCAACCGGTGCTGAAATCGAGAAAGACTATAAAATTCAGAAGAGCATACTCCGTGCAGTTGGCCAGATGGATATCCGAACTGATCTCATGGATGCATATATGTATGCGTTCAAAAGATCTTCTTTGCAGGAGGTTCTAGATCAAAAAGATACTTTCCGAAGCTTAAGGCAAGACGTGTTGCCCTATCTTGTGCGGAGCCAGTTGAGGTCAGAAGTTGTATTGAATCGAACACCCCAAGCGGAAGAAAATGGGAATGAGAAAGTTTCCTCCCATAAGATCCAAGTATTGATTTCACAGATACTTTCTAATTCGTCTACTACAAGTTTTCATGATCTTTATGCATATGGTGCTGATGGATCTATTCCCGTTCGAAAAACACATAAATGTTGTGTTTATATTCCCCCTAGTAGCAAGTATTGTGCTCGCTTATTTTCAATTCAAGCATACAGCGACATTAATCGGGATGTTATTGGAGAAGCAAGCCATCTTTCAGGTTACTCTTTCTCTGCTCAAAATAACATCATTCATCCTTCTGCCCAACTCGGATCAAAAACCACCGTAGGACCCCATTGTATGTTGGGAGAAGGTTCACAGATGGGTGACAAATGTAGCGTGAAACGGTCAGTAATTGGCCAACATTGCCGGATAGGTTCCAATGTGAAGATCGCTAATTCAGTCGTCATGGATCATGTTACCATTGGAGACGGTTGTTCAATTCAAGGTTCGGTTATCTGCAGCAATGTGCAACTTCAGGAGCGCGCTGTTTTAAGAGACTGTCAAGTAGGAGCAGGTTTTGTGGTGACTGCTGCCTGTGAGTACAAAGGAGAGGCGTTGGCTAGAAAAGAAAAGTGA

Protein sequence

MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKDVALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGSVICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Homology
BLAST of Cp4.1LG18g01080 vs. ExPASy Swiss-Prot
Match: P70541 (Translation initiation factor eIF-2B subunit gamma OS=Rattus norvegicus OX=10116 GN=Eif2b3 PE=2 SV=2)

HSP 1 Score: 200.3 bits (508), Expect = 4.8e-50
Identity = 145/466 (31.12%), Postives = 247/466 (53.00%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           M+FQ VV+A G    +  L S  +PK LLPV N+P++ Y L LLE    +++IVV   KD
Sbjct: 1   MEFQAVVMAVGGGSRMTDLTS-SIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTT-KD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPE--DVGTAGALRAIARHLTANDILVVSGDLVSDVS 120
           V       +   +  +L+ ++  +P+  D+GTA +LR I   L   D+LV+  DL++DV+
Sbjct: 61  VQ----KALCAEFKMKLKPDIVCIPDEADMGTADSLRHIYPKL-KTDVLVLGCDLITDVA 120

Query: 121 PGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYI 180
              V    R +DA +  ++        S     G K K K     + +G+D T + LL++
Sbjct: 121 LHEVVDLFRAYDASLAMLMRK---GQESTEPVPGQKGKKKTVEQRDFIGVDSTGKRLLFM 180

Query: 181 ATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVL 240
           A  A+++++  I+ SIL+   ++  +T L+DA++Y  K+  +  +++ K +  S+R +++
Sbjct: 181 ANEADLDEELVIKGSILQKHPRIHFQTGLVDAHLYCLKKYVVDFLMENK-SITSIRSELI 240

Query: 241 PYLVRSQLRSEVVLNRTPQAEENGNEK-VSSHKIQVLISQILSNSSTTSFHDL--YAYGA 300
           PYLVR Q  S     R    EE+  +K   S  I   I +   N+ T + +D    A+  
Sbjct: 241 PYLVRKQFSSASSQQRQEDKEEDLKKKEPKSLDIYSFIKK--DNTLTLAPYDACWNAFRR 300

Query: 301 DGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHP 360
           D    + ++   C         C+R+ ++  Y + NR V    S L       + ++IHP
Sbjct: 301 DKWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSVL-----CPEESMIHP 360

Query: 361 SAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGC 420
           SAQ+ +K  +G   ++G  +Q+G+K S+KRSVIG  C I   V + N ++M+ VT+ +G 
Sbjct: 361 SAQIANKHLIGADSLIGSDTQVGEKSSIKRSVIGSSCVIRDRVTVTNCLLMNSVTVEEGS 420

Query: 421 SIQGSVICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 462
           SI GSVIC+N  ++  A +RDC +G+G  + A  +   E +   ++
Sbjct: 421 SIHGSVICNNAVVEAGAEIRDCLIGSGQRIEAKAKRMNEVIVGNDQ 448

BLAST of Cp4.1LG18g01080 vs. ExPASy Swiss-Prot
Match: Q4R6T3 (Translation initiation factor eIF-2B subunit gamma OS=Macaca fascicularis OX=9541 GN=EIF2B3 PE=2 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 3.4e-48
Identity = 144/470 (30.64%), Postives = 250/470 (53.19%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           M+FQ VV+A G    +  L S  +PK LLP  N+P++ Y L LLE    +++IVV   +D
Sbjct: 1   MEFQAVVMAVGGGSRMTDLTS-SIPKPLLPAGNKPLIWYPLNLLERVGFEEVIVVTT-RD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPE--DVGTAGALRAIARHLTANDILVVSGDLVSDVS 120
           V       +   +  +++ ++  +P+  D+GTA +LR +   L   D+LV+S DL++DV+
Sbjct: 61  VQ----KALCAEFKMKMKPDIVCIPDDADMGTADSLRYMYPKL-KTDVLVLSCDLITDVA 120

Query: 121 PGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYI 180
              V    R +DA +  ++        S     G K K K     + +G+D T + LL++
Sbjct: 121 LHEVVDLFRAYDASLAMLMRK---GQDSLEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFM 180

Query: 181 ATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVL 240
           A  A+++++  I+ SIL+   ++   TDL+DA++Y  K+  + + L +  +  S+R +++
Sbjct: 181 ANEADLDEELVIKGSILQKYPRIRFHTDLVDAHLYCLKK-YVVDFLMENGSITSIRSELI 240

Query: 241 PYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVL-ISQILSNSSTTSF--HDLYAYGA 300
           PYLVR Q  S      + Q +E   E +   +++ L I   L  ++T +   +D      
Sbjct: 241 PYLVRKQFSSA----SSQQGQEEKEEDLKKKELKSLDIYSFLKEANTLNLAPYDACWNAC 300

Query: 301 DG----SIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNN 360
            G     +P R   +C V+I      C+R+ ++  Y + NR V    S L       +  
Sbjct: 301 RGDRWEDLP-RSQVRCYVHIMKEG-LCSRVSTLGLYMEANRQVPKLLSAL-----CPEEP 360

Query: 361 IIHPSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTI 420
           ++H SAQ+ SK  VG   ++G  +Q+G+K S+KRSVIG  C I   V I N ++M+ VT+
Sbjct: 361 LVHSSAQIVSKHLVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTV 420

Query: 421 GDGCSIQGSVICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 462
            +G +IQGSVIC+N  +++ A ++DC +G+G  + A  +   E +   ++
Sbjct: 421 EEGSNIQGSVICNNAVIEKGADIKDCLIGSGQRIEAKAKRVNEVIVGSDQ 448

BLAST of Cp4.1LG18g01080 vs. ExPASy Swiss-Prot
Match: Q9NR50 (Translation initiation factor eIF-2B subunit gamma OS=Homo sapiens OX=9606 GN=EIF2B3 PE=1 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 5.0e-47
Identity = 143/469 (30.49%), Postives = 246/469 (52.45%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           M+FQ VV+A G    +  L S  +PK LLPV N+P++ Y L LLE    +++IVV   +D
Sbjct: 1   MEFQAVVMAVGGGSRMTDLTS-SIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVVTT-RD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPE--DVGTAGALRAIARHLTANDILVVSGDLVSDVS 120
           V       +   +  +++ ++  +P+  D+GTA +LR I   L   D+LV+S DL++DV+
Sbjct: 61  VQ----KALCAEFKMKMKPDIVCIPDDADMGTADSLRYIYPKL-KTDVLVLSCDLITDVA 120

Query: 121 PGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYI 180
              V    R +DA +  ++        S     G K K K     + +G+D T + LL++
Sbjct: 121 LHEVVDLFRAYDASLAMLMRK---GQDSIEPVPGQKGKKKAVEQRDFIGVDSTGKRLLFM 180

Query: 181 ATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVL 240
           A  A+++++  I+ SIL+   ++   T L+DA++Y  K+  + + L +  +  S+R +++
Sbjct: 181 ANEADLDEELVIKGSILQKHPRIRFHTGLVDAHLYCLKK-YIVDFLMENGSITSIRSELI 240

Query: 241 PYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVL-ISQILSNSSTTSFHDLYA----- 300
           PYLVR Q  S      + Q +E   E +   +++ L I   +  ++T +     A     
Sbjct: 241 PYLVRKQFSSA----SSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNAC 300

Query: 301 YGADGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNI 360
            G       R   +C V+I      C+R+ ++  Y + NR V    S L       +   
Sbjct: 301 RGDRWEDLSRSQVRCYVHIMKEG-LCSRVSTLGLYMEANRQVPKLLSAL-----CPEEPP 360

Query: 361 IHPSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIG 420
           +H SAQ+ SK  VG   ++G  +Q+G+K S+KRSVIG  C I   V I N ++M+ VT+ 
Sbjct: 361 VHSSAQIVSKHLVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVE 420

Query: 421 DGCSIQGSVICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 462
           +G +IQGSVIC+N  +++ A ++DC +G+G  + A  +   E +   ++
Sbjct: 421 EGSNIQGSVICNNAVIEKGADIKDCLIGSGQRIEAKAKRVNEVIVGNDQ 448

BLAST of Cp4.1LG18g01080 vs. ExPASy Swiss-Prot
Match: Q54FQ8 (Translation initiation factor eIF-2B subunit gamma OS=Dictyostelium discoideum OX=44689 GN=eif2b3 PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 134/441 (30.39%), Postives = 230/441 (52.15%), Query Frame = 0

Query: 3   FQVVVL----AGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLK---DLIVV 62
           FQVV+L    A G SK L P +   +P +LLP+ANRP++SY LE LE +  +   + +++
Sbjct: 6   FQVVILATDKASGNSK-LEP-IDATIPHSLLPIANRPLISYQLEFLEKAGFETKSEPVII 65

Query: 63  AEGKDVALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLV- 122
              +    +I  ++S  Y  ++ VE   + + + T   L  I   +     +V++ +LV 
Sbjct: 66  VVNETSQEKIKQYVSEIYKGKIEVEFFVLKDQLATCEILYRIRDKIRLEYFMVLNANLVL 125

Query: 123 SDVSPGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKK-AVVHNVVGLDPTKQ 182
            D     +A  HR+ ++ +T +L   P     + G    +  TK+  +  + + L+   Q
Sbjct: 126 EDTFIRQMADLHRKEESSLTVLL-KPPTPKVEQKGKGATETSTKQDKLFTDYIALEEKSQ 185

Query: 183 FLLYIATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVL-DQKD---T 242
            ++ +    E+E+D    KS+L+    + I T+L D  +Y F R  L  ++ DQK+    
Sbjct: 186 KIVMMEPATEVEEDLNFNKSLLKYFPNVTIYTNLQDTQLYIFSRWVLDLIIEDQKEKYPL 245

Query: 243 FRSLRQDVLPYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHD 302
           F  +++ ++PYL+  Q+ +       P +  N N+ +S            + SSTTS  D
Sbjct: 246 FFDIKKHLIPYLLSCQIPNIKRKRALPASAFNQNQTLSQ-----------TMSSTTSPFD 305

Query: 303 LYAYGADGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVI-GEASHLSGYSFSA 362
            +   ++ +    KT KC  ++     YC  + +I+ Y  INRD+  G+  +L     S 
Sbjct: 306 QF---SELNAQKNKTIKCFAHLLKKEGYCMNVNTIKNYQQINRDIAKGDLQYLPNEPKSE 365

Query: 363 QNNIIHPSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDH 422
           +N  I P+A + + T VGP C++G  + +G KCSVK S+IG+HC+IG  V+I NS++MDH
Sbjct: 366 KNFFIDPTANV-TITQVGPQCVIGTSTTLGAKCSVKFSIIGKHCKIGDGVRIENSIIMDH 425

Query: 423 VTIGDGCSIQGSVICSNVQLQ 430
           V I D C I  S+IC++V ++
Sbjct: 426 VIIEDRCVINSSIICNDVYIK 428

BLAST of Cp4.1LG18g01080 vs. ExPASy Swiss-Prot
Match: A5PJI7 (Translation initiation factor eIF-2B subunit gamma OS=Bos taurus OX=9913 GN=EIF2B3 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 4.2e-46
Identity = 140/448 (31.25%), Postives = 244/448 (54.46%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           M+FQ VV+A G    +  L S  +PK LLPV N+P++ Y L LLE    +++IV+   KD
Sbjct: 1   MEFQAVVMAVGGGSRMTDLTS-SIPKPLLPVGNKPLIWYPLNLLERVGFEEVIVITT-KD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPE--DVGTAGALRAIARHLTANDILVVSGDLVSDVS 120
           V   + +  +     ++++++  +P+  D+GTA +LR I + L   D+LV+S DL++DV+
Sbjct: 61  VQKALCADFNKM---KMKLDIVCIPDEADMGTADSLRHIYQKL-KTDVLVLSCDLITDVA 120

Query: 121 PGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYI 180
              V    R HDA +  ++        S     G K K K     + VG+D T + LL++
Sbjct: 121 LHEVVDLFRAHDASLAMLMRK---GQESLEPVPGQKGKKKAVEQRDFVGVDSTGKRLLFM 180

Query: 181 ATGAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVL 240
           A  A+++++  I+ SIL+   ++   T L+DA++Y  K+  +  +++ K +  S+R +++
Sbjct: 181 ANEADLDEELIIKGSILQKHPRIRFHTGLVDAHLYCLKKYVVDFLMENK-SITSIRSELI 240

Query: 241 PYLVRSQLRSEVVLNRTPQAEENGNEK-VSSHKIQVLISQILSNSSTTSFHDLYAYGADG 300
           PYLVR Q  S        + EE+  +K + S  I   I +  +N+ T + +D       G
Sbjct: 241 PYLVRKQFSSASSQQGQEEKEEDLKKKELKSLDIYSFIKE--ANTLTLAPYDTCWNACRG 300

Query: 301 SI---PVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIH 360
                  R   +C V+I      C+R+ ++  Y + NR V    S L       + ++IH
Sbjct: 301 DSWEGLSRSQVRCYVHIMKEG-LCSRVSTLGLYMEANRQV----SKLLPV-ICPEESLIH 360

Query: 361 PSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDG 420
            SAQ+ SK  VG   ++G  +Q+G+K S+K SVIG  C I   V + N ++M+ VT+ +G
Sbjct: 361 SSAQIVSKHMVGADSLIGPDTQVGEKSSIKHSVIGSSCVIRDRVTVTNCLLMNSVTVEEG 420

Query: 421 CSIQGSVICSNVQLQERAVLRDCQVGAG 443
            +IQ S+IC++  +++ A +++C +G+G
Sbjct: 421 SNIQSSIICNDAVIEKGADIKNCLIGSG 430

BLAST of Cp4.1LG18g01080 vs. NCBI nr
Match: XP_023516990.1 (translation initiation factor eIF-2B subunit gamma-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894 bits (2309), Expect = 0.0
Identity = 461/461 (100.00%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP
Sbjct: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. NCBI nr
Match: XP_022988103.1 (translation initiation factor eIF-2B subunit gamma-like [Cucurbita maxima])

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTAN+ILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANNILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVVLNRTPQAEENGNEKVSSHKI+VLISQILSNSSTTSFHDLYAYGADGSIP
Sbjct: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIKVLISQILSNSSTTSFHDLYAYGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGY+FSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYTFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. NCBI nr
Match: KAG6590261.1 (Translation initiation factor eIF-2B subunit gamma, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033455.1 Translation initiation factor eIF-2B subunit gamma [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 886 bits (2290), Expect = 0.0
Identity = 456/461 (98.92%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVV NRTPQAEENGNEKVSSHKIQ LISQILSNSSTTSFHDLYAYGA+GSIP
Sbjct: 241 LVRSQLRSEVVSNRTPQAEENGNEKVSSHKIQALISQILSNSSTTSFHDLYAYGANGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQ+GAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQIGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. NCBI nr
Match: XP_022960703.1 (translation initiation factor eIF-2B subunit gamma-like [Cucurbita moschata])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 455/461 (98.70%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAF+RS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFRRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQ LISQILSNSSTTSFHDLYAYGA+GSI 
Sbjct: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQALISQILSNSSTTSFHDLYAYGANGSIL 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQ+GAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQIGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. NCBI nr
Match: XP_038878285.1 (translation initiation factor eIF-2B subunit gamma [Benincasa hispida])

HSP 1 Score: 847 bits (2189), Expect = 8.57e-309
Identity = 436/461 (94.58%), Postives = 449/461 (97.40%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKD+IVVAEG+D
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDIIVVAEGED 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAI+RHLTANDILVVSGDLVSDV PG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAISRHLTANDILVVSGDLVSDVPPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAA HRR+DAVVTAMLCSVPVSGSSESGS GGKDKTKKAVVHN+VGLDPTKQFLLYIAT
Sbjct: 121 AVAATHRRNDAVVTAMLCSVPVSGSSESGSAGGKDKTKKAVVHNIVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIR+DLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRSDLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVR QLRSEV+ N TP+AEENG EKV SHKIQVL+SQILSNSSTTSFHDLYA GA+GSIP
Sbjct: 241 LVRRQLRSEVLSNGTPRAEENGIEKVGSHKIQVLLSQILSNSSTTSFHDLYASGANGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIP SS YCARLFSIQ+YSDINRDVIGEASHLSGYSFSAQNNII PSAQLG
Sbjct: 301 VRKTHKCCVYIPTSSNYCARLFSIQSYSDINRDVIGEASHLSGYSFSAQNNIIQPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIG+HCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGRHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. ExPASy TrEMBL
Match: A0A6J1JLA6 (translation initiation factor eIF-2B subunit gamma-like OS=Cucurbita maxima OX=3661 GN=LOC111485450 PE=4 SV=1)

HSP 1 Score: 888 bits (2294), Expect = 0.0
Identity = 457/461 (99.13%), Postives = 461/461 (100.00%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTAN+ILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANNILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVVLNRTPQAEENGNEKVSSHKI+VLISQILSNSSTTSFHDLYAYGADGSIP
Sbjct: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIKVLISQILSNSSTTSFHDLYAYGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGY+FSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYTFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. ExPASy TrEMBL
Match: A0A6J1H9R6 (translation initiation factor eIF-2B subunit gamma-like OS=Cucurbita moschata OX=3662 GN=LOC111461419 PE=4 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 455/461 (98.70%), Postives = 459/461 (99.57%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG
Sbjct: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT
Sbjct: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAF+RS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFRRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQ LISQILSNSSTTSFHDLYAYGA+GSI 
Sbjct: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQALISQILSNSSTTSFHDLYAYGANGSIL 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQ+GAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQIGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. ExPASy TrEMBL
Match: A0A0A0M2H0 (NTP_transferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G600810 PE=4 SV=1)

HSP 1 Score: 846 bits (2185), Expect = 1.69e-308
Identity = 433/461 (93.93%), Postives = 448/461 (97.18%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKD+IVVAEG+D
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDIIVVAEGED 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAYD+RLRVEVTSVPEDVGTAGALRAI+RHLTANDILVVSGDLVSDV PG
Sbjct: 61  VALRIGSWISGAYDERLRVEVTSVPEDVGTAGALRAISRHLTANDILVVSGDLVSDVPPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAA HRR+ AVVTAMLCSVPVSGSSESGS GGKDKTKKAV+HN+VGLDPTKQFLLYIA+
Sbjct: 121 AVAATHRRNGAVVTAMLCSVPVSGSSESGSAGGKDKTKKAVIHNIVGLDPTKQFLLYIAS 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIR DLMDAYMYAFKRS+LQ+VLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRADLMDAYMYAFKRSALQKVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEV+LN TP+ EENG EKV SHKIQVL+SQILSNSS TSFHDLYA GADGSIP
Sbjct: 241 LVRSQLRSEVLLNGTPRTEENGLEKVGSHKIQVLLSQILSNSSATSFHDLYASGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSS YCARLFSIQ+YSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSNYCARLFSIQSYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIG+HCRIGSNVKIANSVVMDHVTI DGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGRHCRIGSNVKIANSVVMDHVTIADGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. ExPASy TrEMBL
Match: A0A1S3BRQ0 (translation initiation factor eIF-2B subunit gamma OS=Cucumis melo OX=3656 GN=LOC103492452 PE=4 SV=1)

HSP 1 Score: 845 bits (2182), Expect = 4.84e-308
Identity = 433/461 (93.93%), Postives = 447/461 (96.96%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKD+IVVAEG+D
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDIIVVAEGED 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAY++RLRVEVTSVPEDVGTAGALRAI+RHLTANDILVVSGDLVSDV PG
Sbjct: 61  VALRIGSWISGAYEERLRVEVTSVPEDVGTAGALRAISRHLTANDILVVSGDLVSDVPPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAA HRR+ AVVTAMLCSVPVSGSSESGS  GKDKTKKAVVHN+VGLDPTKQFLLYIAT
Sbjct: 121 AVAATHRRNGAVVTAMLCSVPVSGSSESGSASGKDKTKKAVVHNIVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIR DLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRADLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEV+LN TP+ EENG EKV SHKIQVL+SQILSNSS TSFHDLYA GADGSIP
Sbjct: 241 LVRSQLRSEVLLNGTPRTEENGIEKVGSHKIQVLLSQILSNSSATSFHDLYACGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSS YCARLFSIQ+YSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSNYCARLFSIQSYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIG+HCRIGSNVKIANSVVMDHVTI DGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGRHCRIGSNVKIANSVVMDHVTIADGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAV+RDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVIRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. ExPASy TrEMBL
Match: A0A5A7UM73 (Translation initiation factor eIF-2B subunit gamma OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00630 PE=4 SV=1)

HSP 1 Score: 843 bits (2178), Expect = 1.97e-307
Identity = 432/461 (93.71%), Postives = 446/461 (96.75%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKD+IVVAEG+D
Sbjct: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDIIVVAEGED 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
           VALRIGSWISGAY++RLRVEVTSVPEDVGTAGALRAI+RHLTANDILVVSGDLVSDV PG
Sbjct: 61  VALRIGSWISGAYEERLRVEVTSVPEDVGTAGALRAISRHLTANDILVVSGDLVSDVPPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAA HRR+ AVVTAMLCSVPVSGSSESGS  GKDKTKKAVVHN+VGLDPTKQFLLYIAT
Sbjct: 121 AVAATHRRNGAVVTAMLCSVPVSGSSESGSASGKDKTKKAVVHNIVGLDPTKQFLLYIAT 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           GAEIEKDYKIQKSILRAVGQMDIR DLMDAYMYAFKRS+LQEVLDQKDTFRSLRQDVLPY
Sbjct: 181 GAEIEKDYKIQKSILRAVGQMDIRADLMDAYMYAFKRSALQEVLDQKDTFRSLRQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIP 300
           LVRSQLRSEV+LN TP+ EENG EKV SHKIQVL+SQILSNSS TSFHDLY  GADGSIP
Sbjct: 241 LVRSQLRSEVLLNGTPRTEENGIEKVGSHKIQVLLSQILSNSSATSFHDLYVCGADGSIP 300

Query: 301 VRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360
           VRKTHKCCVYIPPSS YCARLFSIQ+YSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG
Sbjct: 301 VRKTHKCCVYIPPSSNYCARLFSIQSYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQLG 360

Query: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 420
           SKTTVGPHCMLGEGSQMGDKCSVKRSVIG+HCRIGSNVKIANSVVMDHVTI DGCSIQGS
Sbjct: 361 SKTTVGPHCMLGEGSQMGDKCSVKRSVIGRHCRIGSNVKIANSVVMDHVTIADGCSIQGS 420

Query: 421 VICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARKEK 461
           VICSNVQLQERAV+RDCQVGAGFVVTAACEYKGEALARKEK
Sbjct: 421 VICSNVQLQERAVIRDCQVGAGFVVTAACEYKGEALARKEK 461

BLAST of Cp4.1LG18g01080 vs. TAIR 10
Match: AT5G19485.1 (transferases;nucleotidyltransferases )

HSP 1 Score: 641.3 bits (1653), Expect = 5.8e-184
Identity = 329/460 (71.52%), Postives = 381/460 (82.83%), Query Frame = 0

Query: 1   MDFQVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKD 60
           MDFQVV+LAGG S  LVPLV+KE+PKALLPVANRPVLSYVL+LLE SNLKDLIVV EG+D
Sbjct: 1   MDFQVVILAGGFSSYLVPLVAKEVPKALLPVANRPVLSYVLDLLESSNLKDLIVVVEGED 60

Query: 61  VALRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLTANDILVVSGDLVSDVSPG 120
            AL++G WIS A  DRL VEV +V E+VGTAGALRAIA HLTA DIL+VSGD+VSD+ PG
Sbjct: 61  AALKVGGWISSACVDRLHVEVAAVAENVGTAGALRAIAHHLTAKDILIVSGDIVSDIPPG 120

Query: 121 AVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIAT 180
           AVAA HRRHDA VT MLC+ PVSG SESG +GGKDKTKK    +++GLD  KQFLLYIA 
Sbjct: 121 AVAATHRRHDAAVTVMLCAQPVSGPSESGGSGGKDKTKKPACDDIIGLDSLKQFLLYIAK 180

Query: 181 GAEIEKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPY 240
           G EI+KD +++KSIL A G+M+IR+DLMD+++YAFKR+ LQEVLDQK  FRSL+QDVLPY
Sbjct: 181 GTEIKKDTRVKKSILCAAGKMEIRSDLMDSHIYAFKRAVLQEVLDQKPAFRSLKQDVLPY 240

Query: 241 LVRSQLRSEVVLNRTPQAEE-NGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSI 300
           LVR+QLRS+V  +++   E  NGN K +    +V++SQILSN+S  SFH +Y  G    +
Sbjct: 241 LVRTQLRSDVFSDQSNVEENGNGNGKNNMQNNEVVLSQILSNASMPSFHQVYESG----L 300

Query: 301 PVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQNNIIHPSAQL 360
             RKTHKCCVYI   SKY  RL SIQA+ D+NRDVIGE++HLSGYSFSA +NI+HPSA+L
Sbjct: 301 GTRKTHKCCVYIADESKYFVRLNSIQAFMDVNRDVIGESNHLSGYSFSAHHNIVHPSAEL 360

Query: 361 GSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQG 420
           GSKTTVGPHCMLGEGSQ+GDKCSVKRSVIG+HCRIGSNVKI NSVVMDH TIGDGCSIQG
Sbjct: 361 GSKTTVGPHCMLGEGSQVGDKCSVKRSVIGRHCRIGSNVKIVNSVVMDHATIGDGCSIQG 420

Query: 421 SVICSNVQLQERAVLRDCQVGAGFVVTAACEYKGEALARK 460
           SVICSN QLQER  LRDCQV AG+VV A  E+KGE  ARK
Sbjct: 421 SVICSNAQLQERVTLRDCQVEAGYVVCAGSEHKGETFARK 456

BLAST of Cp4.1LG18g01080 vs. TAIR 10
Match: AT2G34970.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 85.9 bits (211), Expect = 9.3e-17
Identity = 112/476 (23.53%), Postives = 195/476 (40.97%), Query Frame = 0

Query: 4   QVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIV--VAEGKDV 63
           Q ++LA   +    P V+ E PK LLP+ N P++ Y L  LE + ++++ V   A    V
Sbjct: 26  QAILLADSFATKFRP-VTLERPKVLLPIVNVPMIDYTLAWLESAGIEEVFVFCCAHSMQV 85

Query: 64  A--LRIGSWISGAYDDRLRVEVTSVPEDVGTAGALRAIARHLT-----ANDILVVSGDLV 123
              L    W S      L V      + +    ALR +    T       D ++VSGD V
Sbjct: 86  IEYLEKSEWYS---HPNLLVRTIESHKSISAGDALRYMYEQQTETSQIQGDFVLVSGDTV 145

Query: 124 SDVSPGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDP-TKQ 183
           S++    +   HR         + ++ +  S  S  T    +++       + +DP TKQ
Sbjct: 146 SNMPLADLIQEHRERKKKDEKAIMTMVIKQSKSSPLT---HQSRLGTDQLFIAVDPLTKQ 205

Query: 184 FLLYIATGAEIEKDYK-----IQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKD 243
            L Y     E + D+      ++KS+L     + +  D+ D Y+       L    D  D
Sbjct: 206 LLHY----EEDKIDHPSGSVCLEKSLLDTNPSVLVCNDMQDCYIDICSPEVLSLFEDNFD 265

Query: 244 TFRSLRQDVLPYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFH 303
            ++ LR+    + V+  L  +++             K+ +H+I                 
Sbjct: 266 -YQHLRR----HFVKGVLVDDIM-----------GYKIFTHEIH---------------- 325

Query: 304 DLYAYGADGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEAS--HLSGYSF 363
                                     S Y  R+ + ++Y  +++D+I   +  ++   +F
Sbjct: 326 --------------------------SSYAGRIDNFRSYDTVSKDIIQRWTYPYVPDINF 385

Query: 364 S-------AQNNIIHPSAQLGSKTT-VGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNV 423
           S        +  I   S  + S++  VG   ++G G+++G    +  SVIG  C IGSNV
Sbjct: 386 SGNRPLKLGRQGIYKASDVVQSRSADVGASTVIGYGTKIGHGDKIMNSVIGNGCSIGSNV 432

Query: 424 KIANSVVMDHVTIGDGCSIQGSVICSNVQLQERAVLR-------DCQVGAGFVVTA 448
            I  S + ++VTI DGC I+ +++C  V+++  AVL+       +  VG  FVV A
Sbjct: 446 VIEGSYIWNNVTIEDGCEIRNAIVCDGVKIRAGAVLQPGVVLSFNVVVGRDFVVPA 432

BLAST of Cp4.1LG18g01080 vs. TAIR 10
Match: AT4G18300.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 82.8 bits (203), Expect = 7.9e-16
Identity = 107/454 (23.57%), Postives = 184/454 (40.53%), Query Frame = 0

Query: 20  VSKELPKALLPVANRPVLSYVLELLELSNLKDLIV--VAEGKDVA--LRIGSWISGAYDD 79
           ++ E PK LLP+ N P++ Y L  LE + ++++ V   A    +   L+   W +   + 
Sbjct: 40  ITLERPKVLLPIVNIPMIDYTLAWLESAGIEEVFVFCCAHSSQIIDYLKKSEWYTRP-NP 99

Query: 80  RLRVEVTSVPEDVGTAGALRAIARHLT-----ANDILVVSGDLVSDVSPGAVAAAHRRHD 139
            L V             ALR I    T       D ++VSGD VS++    +   HR   
Sbjct: 100 NLLVRTIVSHNSTSVGDALRYIYEQQTETSQIQGDFVLVSGDTVSNMPLADLIQQHRDRK 159

Query: 140 AVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLLYIATGAEIEKDYKI 199
                 + ++     S  G   G D+         V +DP  + LL+         D+ +
Sbjct: 160 KKDEKAIMTMVFKKQSRLGI--GSDQL-------FVAVDPLTKQLLHYEECNSRGGDFCL 219

Query: 200 QKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDTFRSLRQDVLPYLVRSQLRSEV 259
            KS+L +     + +D+ D Y+       L    D  D ++ +R+    + V   L  ++
Sbjct: 220 DKSLLDST---VLCSDMQDCYIDICSPEVLSLFEDNFD-YQHMRR----HFVNGLLVDDI 279

Query: 260 VLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHDLYAYGADGSIPVRKTHKCCVY 319
           +             K+ +H+IQ                                      
Sbjct: 280 M-----------GYKIFTHEIQ-------------------------------------- 339

Query: 320 IPPSSKYCARLFSIQAYSDINRDVIGEAS--HLSGYSFS-------AQNNIIHPSAQLGS 379
              SS Y AR+ ++++Y  +++D+I   +  ++    FS        +  I   S  + S
Sbjct: 340 ---SSCYAARIDNLRSYDTVSKDIIQRWTYPYVPNIDFSGNRPVKLGRRGIYRASDAVQS 399

Query: 380 KTT-VGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHVTIGDGCSIQGS 439
            T  VG   ++G G+++G+   +  SVIG  C IGSNV I  S + ++VTI DGC I+ +
Sbjct: 400 HTADVGASTVIGYGTKIGNGGKIYNSVIGNGCCIGSNVVIQGSYIWNNVTIEDGCEIRNA 423

Query: 440 VICSNVQLQERAVLR-------DCQVGAGFVVTA 448
           ++C  V+++  AVL+          VG  FVV A
Sbjct: 460 IVCDGVKVRAGAVLQPGVVLSFSVVVGRDFVVPA 423

BLAST of Cp4.1LG18g01080 vs. TAIR 10
Match: AT3G02270.1 (Trimeric LpxA-like enzyme )

HSP 1 Score: 73.6 bits (179), Expect = 4.8e-13
Identity = 108/465 (23.23%), Postives = 186/465 (40.00%), Query Frame = 0

Query: 4   QVVVLAGGTSKNLVPLVSKELPKALLPVANRPVLSYVLELLELSNLKDLIVVAEGKDV-A 63
           Q ++LA   +  L+PL + E P  LLP+ N P++ Y L  LE + ++++ V    + +  
Sbjct: 26  QAILLADSFATKLLPL-TLERPNVLLPLVNIPMIDYTLAWLESAGIEEVFVFCSMQVIDY 85

Query: 64  LRIGSWISGAYDDRLRVEVTSVPEDVGTAG-ALR-----AIARHLTANDILVVSGDLVSD 123
           L    W S        V+    P++  +AG ALR      I       D ++V+G +VS+
Sbjct: 86  LNNSDWYS---HKDFTVKTIESPQNSTSAGDALRYIYEQQIETSQIQGDFVLVNGCIVSN 145

Query: 124 VSPGAVAAAHRRHDAVVTAMLCSVPVSGSSESGSTGGKDKTKKAVVHNVVGLDPTKQFLL 183
           +    +   HR                          K K +KA++  V+         L
Sbjct: 146 MPLTQLIQEHRDR------------------------KKKDEKAIMTMVIRQSLITDHQL 205

Query: 184 YIATGAEI-------EKDYKIQKSILRAVGQMDIRTDLMDAYMYAFKRSSLQEVLDQKDT 243
           +IA            E +    KS+L     + + +D+ D Y+       L   +D  D 
Sbjct: 206 FIAVNPLTKQLLYYDEDNICFDKSLLDRNPSVLLCSDMQDCYIDICSLEVLSLFVDNFD- 265

Query: 244 FRSLRQDVLPYLVRSQLRSEVVLNRTPQAEENGNEKVSSHKIQVLISQILSNSSTTSFHD 303
           ++ +R D     V   L  +++             K+ +H+I    +  + N  +   +D
Sbjct: 266 YQHMRCD----FVEGVLADDII-----------GYKIFTHEISSCYASRIENFRS---YD 325

Query: 304 LYAYGADGSIPVRKTHKCCVYIPPSSKYCARLFSIQAYSDINRDVIGEASHLSGYSFSAQ 363
           +        I  R+T     Y+P         FS      + R  I +AS  +       
Sbjct: 326 M----VSKDIIQRRTFP---YVPDMK------FSGNRTLKLERQGIYKASDATQL----- 385

Query: 364 NNIIHPSAQLGSKTTVGPHCMLGEGSQMGDKCSVKRSVIGQHCRIGSNVKIANSVVMDHV 423
                PSA +G+   +G    +G G++      +  SVIG  C IGSNV I  S + ++V
Sbjct: 386 -----PSAHVGASYVIGHATNIGSGTK------ILNSVIGNGCSIGSNVVIQGSYIWNNV 414

Query: 424 TIGDGCSIQGSVICSNVQLQERAVLR-------DCQVGAGFVVTA 448
           T+ DGC I+ +++C  V++   A+++          VG  FVV A
Sbjct: 446 TVEDGCEIRNAIVCDEVKVCAGAIVKPGVVLSFKVVVGRDFVVPA 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P705414.8e-5031.12Translation initiation factor eIF-2B subunit gamma OS=Rattus norvegicus OX=10116... [more]
Q4R6T33.4e-4830.64Translation initiation factor eIF-2B subunit gamma OS=Macaca fascicularis OX=954... [more]
Q9NR505.0e-4730.49Translation initiation factor eIF-2B subunit gamma OS=Homo sapiens OX=9606 GN=EI... [more]
Q54FQ81.9e-4630.39Translation initiation factor eIF-2B subunit gamma OS=Dictyostelium discoideum O... [more]
A5PJI74.2e-4631.25Translation initiation factor eIF-2B subunit gamma OS=Bos taurus OX=9913 GN=EIF2... [more]
Match NameE-valueIdentityDescription
XP_023516990.10.0100.00translation initiation factor eIF-2B subunit gamma-like [Cucurbita pepo subsp. p... [more]
XP_022988103.10.099.13translation initiation factor eIF-2B subunit gamma-like [Cucurbita maxima][more]
KAG6590261.10.098.92Translation initiation factor eIF-2B subunit gamma, partial [Cucurbita argyrospe... [more]
XP_022960703.10.098.70translation initiation factor eIF-2B subunit gamma-like [Cucurbita moschata][more]
XP_038878285.18.57e-30994.58translation initiation factor eIF-2B subunit gamma [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1JLA60.099.13translation initiation factor eIF-2B subunit gamma-like OS=Cucurbita maxima OX=3... [more]
A0A6J1H9R60.098.70translation initiation factor eIF-2B subunit gamma-like OS=Cucurbita moschata OX... [more]
A0A0A0M2H01.69e-30893.93NTP_transferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G60... [more]
A0A1S3BRQ04.84e-30893.93translation initiation factor eIF-2B subunit gamma OS=Cucumis melo OX=3656 GN=LO... [more]
A0A5A7UM731.97e-30793.71Translation initiation factor eIF-2B subunit gamma OS=Cucumis melo var. makuwa O... [more]
Match NameE-valueIdentityDescription
AT5G19485.15.8e-18471.52transferases;nucleotidyltransferases [more]
AT2G34970.19.3e-1723.53Trimeric LpxA-like enzyme [more]
AT4G18300.17.9e-1623.57Trimeric LpxA-like enzyme [more]
AT3G02270.14.8e-1323.23Trimeric LpxA-like enzyme [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 1..339
e-value: 1.1E-33
score: 118.7
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 4..425
NoneNo IPR availableGENE3D2.160.10.10Hexapeptide repeat proteinscoord: 347..459
e-value: 1.4E-18
score: 68.7
NoneNo IPR availablePANTHERPTHR45989TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMAcoord: 1..460
NoneNo IPR availableCDDcd04652LbH_eIF2B_gamma_Ccoord: 370..447
e-value: 1.932E-34
score: 121.916
IPR005835Nucleotidyl transferase domainPFAMPF00483NTP_transferasecoord: 6..147
e-value: 6.3E-12
score: 45.6
IPR001451Hexapeptide repeatPFAMPF00132Hexapepcoord: 391..419
e-value: 1.9E-5
score: 24.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG18g01080.1Cp4.1LG18g01080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002183 cytoplasmic translational initiation
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0009058 biosynthetic process
cellular_component GO:0005851 eukaryotic translation initiation factor 2B complex
cellular_component GO:0032045 guanyl-nucleotide exchange factor complex
molecular_function GO:0005085 guanyl-nucleotide exchange factor activity
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0003743 translation initiation factor activity