Cp4.1LG17g06940 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g06940
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 ubiquitin-protein ligase MIEL1
Location	Cp4.1LG17: 6349164 .. 6353246 (+)
RNA-Seq Expression	Cp4.1LG17g06940
Synteny	Cp4.1LG17g06940

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTCTCGAACCAAACCAGAGAGAACGAACAGGGTCGGTCTCCAATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGGTACCAATTTCTTTCCTTCATGAGGCGGCCTTGGCCTTCTTCTTCTTGTTCATCTTCGTTTTCGTCATCAGTAACTTCTTTCGATTTGAATTAGATGCGAGCATTATAGAAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGTCATTGCCACAACGAGGCCATGGTATTTCTCTCTCCTTTCTGCGTTGTGTGTGCGTGTGAGAGAGGCGTGTAATAAGGAATTGTGTATATGTTGTTGAACTGGAGCTTTCAGCTGAATTTATGAAATTTTCATTGGTTTTAGAGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTCTGTTTGTACGCTGTTCTATTGATTTTTTTTTTCGGTTAAATTGTTGATTGTAACACATCGTGTAAAGGACGTCATAATAGGATTTGATTGATTTTGTTGGATTTGATGCTTTAGGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGTTTGGGGGTTTCTTTCCTTTTCTTTTTGGATAATTTCATTCTTTGTGTTTATATGTTCTTGTTTATGTATTAAACGTTTGAATTTGGTGCATTTTAGGCCGTCAGGGTTTGTCGCAACTGTAATGTGAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTACGATGATGATGTAGGAAAAGTTTCCATCAATCTGTTTCCCTTTATTTAGGAACTGATTTTGAATTTTGAAGTGTGGAACTGAATTATCTGGATGCTAATAGTTTCTGTTGTGTATGTAGACCAAGAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGGTAAGGGTGCAATTTTCCGGTTCTAATTCTAATGTAAACTCAGCTTTCTGTGTTAAGTTTCTAAATAAGTGTTCTTTGTAATATGATTTTCAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGTATGTTATTCAACTCTCTTGATGATTCGTTATAACTTTGTCTTGTGTTGTAACTTCTTTTACTACAAGCAATTACTATAAGTTATTTGCTTATGATACCTTCAGGGTTAGTGCAACTAATTCACTGCCTCTTAAGGTCTTTTTGTCCCTTTGTTTTTGGAGAAGTTATTTGATGAGACCTTTCACAATGCAACCGATAAGTTTGTGTGAGATCCCACATCGGTTGGAGGGGATGGAAACCTCTCCCTAACAGACACGTTTTAAAACCTTGAAGGAAAGCCCGAAAGGAAAATCCAAAAAGGATAATATTTACTAGTCGTGGACAGTTTGCCCTTTTGGATGTGTGAGAGGGAATTTTTTTTAAGACAATATTTAATAACACATTTAGAGCTCATCCAACCATTATAACAAACTAAAAGCTAACCTCACCACTGAACAAGTTTTGGAATTGCAAATTGGAAAGACAACTACACATCTATCTCCTTTAGGGCACAAGTGAAATGAGTTAGCTATAGGTGTCGGTTGCTCTGGTGTAGATCTGTTGACTGACATGAGCTGAGATCATTCTGATAGAACCTCATGCAAGCAAGCCATTGATCCTCAACCAATCAGAAAGGATTGAACTAGCTTGTACTTGGTAACTTCAAAAAGAAGCTAATCATAAGTTCCATTATCAAATGAAACCATGTTGTTCACCTTCTCCTTTTGCTTACCCTTGCCCCCATTTCTTCTTCTGCTGCTGCTTCCTCCTTGTTCGGCGCCACAAGATCTGATTTGAGCATTTGAGTGAAGGAGTTGCAAAGGTTTTGGCTTGAACAAAGACGTGCGAGAGAGAGATTATAAAAGTATTTCATTTTTGTATCCAATAACTTATTTTTGGATCTACATCTTACAGTTATTAAGAGGTGGCCTCATTGAATAATTTGTTCTATTTTTGTGTTTGTGAGGTGGGTCGATAGGGAGATTAACATTTTGTTATAAGTACAGGATCTTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCCATCTGTTATGAGGTTTGTGCTTTTAGATTGCGTATTTTCCCAGGAAGGTACAATCTTAACTGTTCTCTTCTAACAACTTTATTGATGCTTGCAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAGTACTTGAATTCTCAACTCAACATCCAGTTTCTGAATATACTAAAATATTTCTAAAAACTTTGCAAATACAAAATACAACTCTATTATATCCATTGTCTTTATTCTCAAACCAAACAGGTTCTAAGCTTCCTGTTTTGAATTACATTGAGATTTCCAGTTCTCTTATTCTAATGTAGTAATATTTGCCTCTCAGATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGGTGTGTAACACTTAGTTCAGTCATGATAAAAATGAAATGTAGAAGGAAATTAGAGAACTGTCGTTTTAATTCTTAGAGAGGTTTCCCCAGTTAGTGAACACGATAGAACCGGTGGCTTCACATAAATTTTAGCAACCTTAGAGATTAGAATCTGTATCTTATCATTGTACATTGTACCCCCACTCTTCAACCTTCTGACTGATTTTTTTCATTTTTTTTCTTTTTGTCGTGATGTATGATTACATGTATTGGTCTGTTCGTTAGAAAGTTGGTGGTTGCAGACTTATTATCTTAATAGAAATCTGACTGGAGTGATTGTCTGTTAGAAAGAAAATGAGTGTAGATTCAAGGTTTTAGTTATGCATTTCAGTTGAATTGCGAGAGAGGGATTGAGAGGCAAGATGAAAGAAGCAGTCCATGAGAGCTTAATACTTTCTGTTTCGGTTTGACTTGCTCTGTTTACGACTTTAAATTTTGTCCTTTTCCATTTGCCCATTACCCGTTACCCAGTTGATATTTATGGTAAAATCGATCCTGTTGAATACTAAACTTTTCGGGTCACCGAGTGGTGGTTCTTTATATTTGTAAAGACATGTTTCTTCTGTCATATTTCAGTGTTGATATTCCGAAGTTTCTTTGGTTTCTTAATTGTGTTTACTGTACCGTAGACCTTTGGCATTGCATATTATCTTAAATTTTAAATTCTATTCTATCTTTTTCTAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTAGTTGCTTATCTGCTTGTTTACGTTACCACACTGCATTTCAGAAATCGTCTAACTTAATCAACTACCGGGTTAAACAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTCCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGAAAACTAAAGGTAAGCAAGAAAAATGGGGGCTCTCTTGATTTAACCATTAGGTTCTGTTCAAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGGAAGGCAACCTGTTTTGTTCAGAGGCCGGCTATGATTGGTTTATACCCTTAATCAGACATGTGGAAAGTCTAATAGTGCAACATAAAGTTAAATAGTTGGATCTTCTATAGGAAGAGTCTCAGATTCTTTTCATTGTATCATTTAGTATTTATCTTGCAAAAAAATATATACATCATATTAGATACATTACGCAGATGTGAATTTAACATAAATCTCAACATATTTGATACCTGAATTTCAGTAAATTAGGAAATAGGTTGATGATTGTTCATTTAAGAGGATATAGTCAAGGAGATGGATGATAAAGTGCTTTTAACTCTACTGATAGTTCGTTCGGATGCTGAGTAAACTCGTAGGATATTA

mRNA sequence

TATTCTCGAACCAAACCAGAGAGAACGAACAGGGTCGGTCTCCAATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGATGCGAGCATTATAGAAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGTCATTGCCACAACGAGGCCATGAGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGCCGTCAGGGTTTGTCGCAACTGTAATGTGAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTACGATGATGATACCAAGAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGATCTTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCCATCTGTTATGAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTCCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGAAAACTAAAGGTAAGCAAGAAAAATGGGGGCTCTCTTGATTTAACCATTAGGTTCTGTTCAAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGAAGTTCGATTGTTCGAGTCATTTGATCCATTTAGGAAGGCAACCTGTTTTGTTCAGAGGCCGGCTATGATTGGTTTATACCCTTAATCAGACATGTGGAAAGTCTAATAGTGCAACATAAAGTTAAATAGTTGGATCTTCTATAGGAAGAGTCTCAGATTCTTTTCATTGTATCATTTAGTATTTATCTTGCAAAAAAATATATACATCATATTAGATACATTACGCAGATGTGAATTTAACATAAATCTCAACATATTTGATACCTGAATTTCAGTAAATTAGGAAATAGGTTGATGATTGTTCATTTAAGAGGATATAGTCAAGGAGATGGATGATAAAGTGCTTTTAACTCTACTGATAGTTCGTTCGGATGCTGAGTAAACTCGTAGGATATTA

Coding sequence (CDS)

ATGGAACAAGACGACTGCCCTGTGCCCCAATCTGCCGGGAGAGAAGATTTCGGGAAATTGCAGCACGGATGCGAGCATTATAGAAGACGATGCAGAATCCGAGCCCCGTGCTGCGACGGAATCTTTACCTGTCGTCATTGCCACAACGAGGCCATGAGCTCGCTGAGCAATCCGAAGGACCGCCACGAACTTGTTCGACAGGACGTTAGACAAGTGGTTTGCTTGATTTGCAACACAGAGCAAGAGGCCGTCAGGGTTTGTCGCAACTGTAATGTGAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTACGATGATGATACCAAGAAGAAGCAGTTTCATTGTGATGCTTGTGGTATCTGTAGAGTTGGTGGTCGTGAAAACTTCTTTCACTGTGAAAAGTGTGGATCTTGCTATTCCACCCCGCTGCGCGATAACCACATGTGTGTTGAGAACTCTATGAAAAGTTTTTGTCCCATCTGTTATGAGTTTCTTTTTGACTCAATTAAAGACACAACTGTTATGCCTTGTGGTCACACAATACATTTGGAGTGCTTCAGAGAGATGGAATCACAGAATCAATACCGTTGTCCTATCTGCTCCAAGACAGTTATGGACATGTCTGCAAGCTGGGCATTGCTGAAAATGGAGATTGACTGCACACCGATGCCTGAGGAATACAGCCATGAGGTATCGATTCTCTGCAACGACTGTAGCCATACGAGCAATGTCCGTTTTCACATTTTGGGACACAAGTGCAGTCAGTGCAATTCATTCAATACTAGGAGGATTTCTACTGTAGAAGGTCAATGA

Protein sequence

MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRFHILGHKCSQCNSFNTRRISTVEGQ

Homology

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BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 4.7e-106
Identity = 164/254 (64.57%), Postives = 205/254 (80.71%), Query Frame = 0


Query: 14  REDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVV 73
           R  FGK+  GC+HY+RRC+IRAPCC+ +F CRHCHNE+ S+L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ A +VC NC VNMGEYFC IC FYDDDT+K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSNVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T+ V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 9.6e-91
Identity = 142/251 (56.57%), Postives = 179/251 (71.31%), Query Frame = 0


Query: 16  DFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLS-NPKDRHELVRQDVRQVVC 75
           + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RHEL R +V +V+C
Sbjct: 19  EIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVIC 78

Query: 76  LICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCE 135
            +C TEQ+  + C NC V MG+YFC  CKF+DDD  KKQ+HCD CGICR GG ENFFHC+
Sbjct: 79  SLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHCK 138

Query: 136 KCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQN 195
           +C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+HLEC ++M   N
Sbjct: 139 RCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLHN 198

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSNVRFHILGHK 255
           +Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC   +NVRFH++ HK
Sbjct: 199 RYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAHK 258

Query: 256 CSQCNSFNTRR 265
           CS C S+NTR+
Sbjct: 259 CSSCGSYNTRQ 269

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 6.2e-90
Identity = 142/258 (55.04%), Postives = 188/258 (72.87%), Query Frame = 0


Query: 9   PQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLS-NPKDRHELVRQ 68
           P    + + G +Q+GC HYRRRCRIRAPCC+ IF CRHCHNE  +S+  +   RHEL R 
Sbjct: 46  PDDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVKRHELPRH 105

Query: 69  DVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGR 128
           +V+QV+C +C TEQE  +VC +C V MG+YFC++CK +DDD  K+Q+HC+ CGICR+GG+
Sbjct: 106 EVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCGICRIGGK 165

Query: 129 ENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECF 188
           ENFFHC KCG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+PCGHTIH++C 
Sbjct: 166 ENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGHTIHVKCL 225

Query: 189 REMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSNVRF 248
           REME   Q+ CP+CSK+V DMS +W  L  E+  T      +  V ILCNDC  TS V+F
Sbjct: 226 REMEEHCQFACPLCSKSVCDMSKAWERLDEEL-ATISDTCDNKMVRILCNDCGATSEVQF 285

Query: 249 HILGHKCSQCNSFNTRRI 266
           H++ HKC +C S+NTR+I
Sbjct: 286 HLIAHKCQKCKSYNTRQI 302

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 330.5 bits (846), Expect = 1.8e-89
Identity = 145/266 (54.51%), Postives = 183/266 (68.80%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           ME DD      A R  FG++  GC+HYRRRCRIRAPCC+ +F CRHCHNE      + KD
Sbjct: 1   MELDD------ASRHGFGRMGFGCKHYRRRCRIRAPCCNDVFHCRHCHNE------STKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
            HEL R  V  V+CL+C+TEQ   +VC NC V MGEYFC  CKF+DDD  ++ FHC  CG
Sbjct: 61  GHELDRHAVESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGG++NFFHCEKCGSCYS  LRD H C+ENSMK+ CPICYE+LFDS+++T+V+ CGH
Sbjct: 121 ICRVGGKDNFFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           T+HL+CF EM   +++ CPICS  + DM      L  EI+   +  +Y  +  I+CNDC 
Sbjct: 181 TMHLQCFHEMLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHIDYMGKGWIVCNDCR 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIS 267
            T+ V   + GHKC  C S NT R++
Sbjct: 241 DTTQVYARVAGHKCCHCQSHNTCRVA 254

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.1e-65
Identity = 112/241 (46.47%), Postives = 152/241 (63.07%), Query Frame = 0


Query: 23  GCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVVCLICNTEQE 82
           GCEHY R C ++APCCD ++TCR CH        +  + H+L R  V++V C+ C   Q 
Sbjct: 19  GCEHYDRACLLKAPCCDKLYTCRLCH--------DTNEDHQLDRFKVKEVQCINCEKLQH 78

Query: 83  AVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYST 142
           A + C +C+   GEY+C IC  +D D  K+Q+HC++CGICR+G +E+FFHC KC  C +T
Sbjct: 79  AQQTCEDCSTLFGEYYCSICHLFDKD--KRQYHCESCGICRIGPKEDFFHCLKCNLCLTT 138

Query: 143 PLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICS 202
            LR  H C+EN  +  CPIC E +  S     V+PCGH +H  C+ EM  +  YRCP+C 
Sbjct: 139 NLRGKHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEG-YRCPLCM 198

Query: 203 KTVMDMSASWALLKMEIDCTPMPEEYSH-EVSILCNDCSHTSNVRFHILGHKCSQCNSFN 262
            + +DM+  W  L  E+  TPMP EY +  V ILCNDC+  S V+FHILG KC  C+S+N
Sbjct: 199 HSALDMTRYWRQLDTEVAQTPMPSEYQNVTVDILCNDCNGRSTVQFHILGMKCKLCDSYN 248

[Close]

BLAST of Cp4.1LG17g06940 vs. NCBI nr
Match: XP_023004622.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquitin-protein ligase MIEL1 [Cucurbita pepo subsp. pepo] >KAG6593162.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595 bits (1534), Expect = 2.16e-215
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271
           HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271

[Close]

BLAST of Cp4.1LG17g06940 vs. NCBI nr
Match: XP_022959587.1 (E3 ubiquitin-protein ligase MIEL1 [Cucurbita moschata])

HSP 1 Score: 592 bits (1525), Expect = 5.10e-214
Identity = 269/271 (99.26%), Postives = 270/271 (99.63%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNP D
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPND 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271
           HTSNVRFHILGHKCS+CNSFNTRRISTVEGQ
Sbjct: 241 HTSNVRFHILGHKCSRCNSFNTRRISTVEGQ 271

[Close]

BLAST of Cp4.1LG17g06940 vs. NCBI nr
Match: XP_022953225.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 558 bits (1437), Expect = 1.44e-200
Identity = 253/267 (94.76%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. NCBI nr
Match: XP_022992470.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima])

HSP 1 Score: 556 bits (1434), Expect = 4.14e-200
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. NCBI nr
Match: XP_023549279.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556 bits (1433), Expect = 5.88e-200
Identity = 252/267 (94.38%), Postives = 256/267 (95.88%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+K QFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCSKCNVNMGEYFCGICKFYDDDTRKMQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 595 bits (1534), Expect = 1.05e-215
Identity = 271/271 (100.00%), Postives = 271/271 (100.00%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271
           HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ
Sbjct: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL
Match: A0A6J1H4Y6 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 PE=4 SV=1)

HSP 1 Score: 592 bits (1525), Expect = 2.47e-214
Identity = 269/271 (99.26%), Postives = 270/271 (99.63%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNP D
Sbjct: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPND 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRISTVEGQ 271
           HTSNVRFHILGHKCS+CNSFNTRRISTVEGQ
Sbjct: 241 HTSNVRFHILGHKCSRCNSFNTRRISTVEGQ 271

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL
Match: A0A6J1GP21 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC111455836 PE=4 SV=1)

HSP 1 Score: 558 bits (1437), Expect = 6.99e-201
Identity = 253/267 (94.76%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKSCCKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL
Match: A0A6J1JVS4 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC111488790 PE=4 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 2.00e-200
Identity = 252/267 (94.38%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           MEQ+D PVPQSAGREDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAM+SLSNPKD
Sbjct: 1   MEQEDSPVPQSAGREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMTSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RH+LVRQDVRQVVCLICNTEQE  + C  CNVNMGEYFC ICKFYDDDT+KKQFHCDACG
Sbjct: 61  RHDLVRQDVRQVVCLICNTEQEVSKSCSKCNVNMGEYFCGICKFYDDDTRKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL
Match: A0A6J1D947 (E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400 PE=4 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 4.23e-199
Identity = 251/267 (94.01%), Postives = 257/267 (96.25%), Query Frame = 0


Query: 1   MEQDDCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKD 60
           ME++D  VPQSA REDFGK QHGCEHYRRRCRIRAPCCD IFTCRHCHNEAMSSLSNPKD
Sbjct: 1   MEKEDSSVPQSARREDFGKFQHGCEHYRRRCRIRAPCCDRIFTCRHCHNEAMSSLSNPKD 60

Query: 61  RHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACG 120
           RHELVRQDVRQVVCLICNTEQE  +VCR CNVNMGEYFCDICKF+DDDTKKKQFHCDACG
Sbjct: 61  RHELVRQDVRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFFDDDTKKKQFHCDACG 120

Query: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGH 180
           ICRVGGRENFFHCEKCGSCYSTPLRDNH+CVENSMKSFCPICYEFLFDSIKDTTVM CGH
Sbjct: 121 ICRVGGRENFFHCEKCGSCYSTPLRDNHLCVENSMKSFCPICYEFLFDSIKDTTVMRCGH 180

Query: 181 TIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240
           TIHL+CFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS
Sbjct: 181 TIHLDCFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCS 240

Query: 241 HTSNVRFHILGHKCSQCNSFNTRRIST 267
           HTS VRFHILGHKCSQCNSFNTRRIST
Sbjct: 241 HTSKVRFHILGHKCSQCNSFNTRRIST 267

[Close]

BLAST of Cp4.1LG17g06940 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 4.8e-114
Identity = 173/263 (65.78%), Postives = 211/263 (80.23%), Query Frame = 0


Query: 5   DCPVPQSAGREDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKDRHEL 64
           D  +P+    +DFGK Q GCEHY+RRC+IRAPCC+ IF+CRHCHN++ +SL +PK+RH+L
Sbjct: 19  DSSIPRD---KDFGKFQFGCEHYKRRCKIRAPCCNLIFSCRHCHNDSANSLPDPKERHDL 78

Query: 65  VRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRV 124
           VRQ+V+QVVC IC TEQE  +VC NC VNMGEYFCDICKF+DDD  K+QFHCD CGICRV
Sbjct: 79  VRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQFHCDDCGICRV 138

Query: 125 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 184
           GGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+CYE+LFDS+K   VM CGHT+H+
Sbjct: 139 GGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAAHVMKCGHTMHM 198

Query: 185 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEVSILCNDCSHTSN 244
           +CF +M ++NQYRCPIC+K+++DMS SW LL  EI  T MP EY  EVSILCNDC+  S 
Sbjct: 199 DCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEISATEMPVEYKFEVSILCNDCNKGSK 258

Query: 245 VRFHILGHKCSQCNSFNTRRIST 268
             FHILGHKCS C S+NTRRIST
Sbjct: 259 AMFHILGHKCSDCGSYNTRRIST 278

[Close]

BLAST of Cp4.1LG17g06940 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 385.6 bits (989), Expect = 3.4e-107
Identity = 164/254 (64.57%), Postives = 205/254 (80.71%), Query Frame = 0


Query: 14  REDFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLSNPKDRHELVRQDVRQVV 73
           R  FGK+  GC+HY+RRC+IRAPCC+ +F CRHCHNE+ S+L N  DRH+LVRQDV+QV+
Sbjct: 8   RLHFGKMGFGCKHYKRRCQIRAPCCNEVFDCRHCHNESTSTLRNIYDRHDLVRQDVKQVI 67

Query: 74  CLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHC 133
           C +C+TEQ A +VC NC VNMGEYFC IC FYDDDT+K+QFHCD CGICRVGGRENFFHC
Sbjct: 68  CSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHC 127

Query: 134 EKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQ 193
           +KCGSCY+  LR+NH CVENSM+  CPICYE+LFDS+KDT VM CGHT+H+EC+ EM  +
Sbjct: 128 KKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKR 187

Query: 194 NQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEY-SHEVSILCNDCSHTSNVRFHILGH 253
           +++ CPICS++V+DMS +W  L  EI+ T MP +Y   +V ILCNDC+ T+ V FHI+G 
Sbjct: 188 DKFCCPICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQ 247

Query: 254 KCSQCNSFNTRRIS 267
           KC  C S+NTR I+
Sbjct: 248 KCGHCRSYNTRAIA 261

[Close]

BLAST of Cp4.1LG17g06940 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 338.6 bits (867), Expect = 4.7e-93
Identity = 144/251 (57.37%), Postives = 181/251 (72.11%), Query Frame = 0


Query: 16  DFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLS-NPKDRHELVRQDVRQVVC 75
           D G +++GC HYRRRC IRAPCC+ IF C HCH EA ++++ + K RH++ R  V QV+C
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQVIC 114

Query: 76  LICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCE 135
           L+C TEQE  ++C +C V MG+YFC +CK YDDDT KKQ+HCD CGICR+GGRENFFHC 
Sbjct: 115 LLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFFHCY 174

Query: 136 KCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQN 195
           KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH +C  EM    
Sbjct: 175 KCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMRDHY 234

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSNVRFHILGHK 255
           QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   + V++H++  K
Sbjct: 235 QYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVVAQK 294

Query: 256 CSQCNSFNTRR 265
           C  C S+NTR+
Sbjct: 295 CPNCKSYNTRQ 305

[Close]

BLAST of Cp4.1LG17g06940 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 334.7 bits (857), Expect = 6.8e-92
Identity = 142/251 (56.57%), Postives = 179/251 (71.31%), Query Frame = 0


Query: 16  DFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLS-NPKDRHELVRQDVRQVVC 75
           + G   +GC HYRRRC+IRAPCCD IF CRHCHNEA  SL      RHEL R +V +V+C
Sbjct: 19  EIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHELPRHEVSKVIC 78

Query: 76  LICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCE 135
            +C TEQ+  + C NC V MG+YFC  CKF+DDD  KKQ+HCD CGICR GG ENFFHC+
Sbjct: 79  SLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRTGGEENFFHCK 138

Query: 136 KCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQN 195
           +C  CYS  + D H CVE +M   CP+C+E+LFDS +D TV+ CGHT+HLEC ++M   N
Sbjct: 139 RCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHLECTKDMGLHN 198

Query: 196 QYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTSNVRFHILGHK 255
           +Y CP+CSK++ DMS  W  L  E+   PMP+ Y ++ V ILCNDC   +NVRFH++ HK
Sbjct: 199 RYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNTNVRFHLIAHK 258

Query: 256 CSQCNSFNTRR 265
           CS C S+NTR+
Sbjct: 259 CSSCGSYNTRQ 269

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BLAST of Cp4.1LG17g06940 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 330.5 bits (846), Expect = 1.3e-90
Identity = 144/261 (55.17%), Postives = 181/261 (69.35%), Query Frame = 0


Query: 16  DFGKLQHGCEHYRRRCRIRAPCCDGIFTCRHCHNEAMSSLS-NPKDRHELVRQDVR---- 75
           D G +++GC HYRRRC IRAPCC+ IF C HCH EA ++++ + K RH++ R  V     
Sbjct: 55  DRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQLTR 114

Query: 76  ------QVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRV 135
                 QV+CL+C TEQE  ++C +C V MG+YFC +CK YDDDT KKQ+HCD CGICR+
Sbjct: 115 PLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRI 174

Query: 136 GGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHL 195
           GGRENFFHC KCG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PCGHTIH 
Sbjct: 175 GGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQ 234

Query: 196 ECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHE-VSILCNDCSHTS 255
           +C  EM    QY CP+CSK+V DMS  W    MEI  TPMPE Y +  V ILCNDC   +
Sbjct: 235 KCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKA 294

Query: 256 NVRFHILGHKCSQCNSFNTRR 265
            V++H++  KC  C S+NTR+
Sbjct: 295 EVQYHVVAQKCPNCKSYNTRQ 315

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g06940 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZK0	4.7e-106	64.57	E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ...	[more]
Q9FFB6	9.6e-91	56.57	E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=...	[more]
Q5JL96	6.2e-90	55.04	Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q10LI1	1.8e-89	54.51	E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR...	[more]
Q9CR50	3.1e-65	46.47	RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1...	[more]

BLAST of Cp4.1LG17g06940 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023004622.1	2.16e-215	100.00	E3 ubiquitin-protein ligase MIEL1 [Cucurbita maxima] >XP_023513585.1 E3 ubiquiti...	[more]
XP_022959587.1	5.10e-214	99.26	E3 ubiquitin-protein ligase MIEL1 [Cucurbita moschata]	[more]
XP_022953225.1	1.44e-200	94.76	E3 ubiquitin-protein ligase MIEL1-like [Cucurbita moschata] >KAG6575860.1 E3 ubi...	[more]
XP_022992470.1	4.14e-200	94.38	E3 ubiquitin-protein ligase MIEL1-like [Cucurbita maxima]	[more]
XP_023549279.1	5.88e-200	94.38	E3 ubiquitin-protein ligase MIEL1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cp4.1LG17g06940 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQY2	1.05e-215	100.00	E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE...	[more]
A0A6J1H4Y6	2.47e-214	99.26	E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita moschata OX=3662 GN=LOC111460617 ...	[more]
A0A6J1GP21	6.99e-201	94.76	E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1JVS4	2.00e-200	94.38	E3 ubiquitin-protein ligase MIEL1-like OS=Cucurbita maxima OX=3661 GN=LOC1114887...	[more]
A0A6J1D947	4.23e-199	94.01	E3 ubiquitin-protein ligase MIEL1 OS=Momordica charantia OX=3673 GN=LOC111018400...	[more]

BLAST of Cp4.1LG17g06940 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62970.1	4.8e-114	65.78	zinc finger (C3HC4-type RING finger) family protein	[more]
AT5G18650.1	3.4e-107	64.57	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G25560.1	4.7e-93	57.37	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G22920.1	6.8e-92	56.57	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]
AT5G25560.3	1.3e-90	55.17	CHY-type/CTCHY-type/RING-type Zinc finger protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 159..201 e-value: 7.1E-8 score: 42.2
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 159..201 e-value: 4.0E-8 score: 33.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 159..202 score: 12.282736
IPR039512	RCHY1, zinc-ribbon	PFAM	PF14599	zinc_ribbon_6	coord: 206..263 e-value: 2.0E-23 score: 82.1
None	No IPR available	GENE3D	2.20.28.10		coord: 229..271 e-value: 8.2E-18 score: 65.4
None	No IPR available	PANTHER	PTHR21319	RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1	coord: 15..265
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 158..209
IPR008913	Zinc finger, CHY-type	PFAM	PF05495	zf-CHY	coord: 24..104 e-value: 5.0E-20 score: 71.8
IPR008913	Zinc finger, CHY-type	PROSITE	PS51266	ZF_CHY	coord: 17..92 score: 23.863016
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 141..211 e-value: 7.0E-11 score: 43.3
IPR031109	Ring finger and CHY zinc finger domain-containing protein, plant	PANTHER	PTHR21319:SF12	ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEIN	coord: 15..265
IPR017921	Zinc finger, CTCHY-type	PROSITE	PS51270	ZF_CTCHY	coord: 94..158 score: 33.139317
IPR037274	Zinc finger, CHY-type superfamily	SUPERFAMILY	161219	CHY zinc finger-like	coord: 20..93
IPR037275	Zinc finger, CTCHY-type superfamily	SUPERFAMILY	161245	Zinc hairpin stack	coord: 95..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g06940.1	Cp4.1LG17g06940.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005634	nucleus
molecular_function	GO:0016746	acyltransferase activity
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding