Cp4.1LG17g06880 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG17g06880 vs. ExPASy Swiss-Prot
Match: Q8TB22 (Spermatogenesis-associated protein 20 OS=Homo sapiens OX=9606 GN=SPATA20 PE=2 SV=3)

HSP 1 Score: 560.1 bits (1442), Expect = 4.1e-158
Identity = 315/775 (40.65%), Postives = 439/775 (56.65%), Query Frame = 0

Query: 60  GGSHSH------KYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGY 119
           GG  SH      +  N L  E SPYLLQHA+NPV+WYPWG EAF++ARK N PIFLS+GY
Sbjct: 48  GGKGSHPSSTPQRVPNRLIHEKSPYLLQHAYNPVDWYPWGQEAFDKARKENKPIFLSVGY 107

Query: 120 STCHWCHVMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLS 179
           STCHWCH+ME ESF+NEE+ +LL++ F+S+KVDREERPDVDKVYMT+VQA  SGGGWP++
Sbjct: 108 STCHWCHMMEEESFQNEEIGRLLSEDFVSVKVDREERPDVDKVYMTFVQATSSGGGWPMN 167

Query: 180 VFLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEAL 239
           V+L+P+L+P +GGTYFPP+D   R GF+TVL ++++ W   ++ L+++     ++++ AL
Sbjct: 168 VWLTPNLQPFVGGTYFPPEDGLTRVGFRTVLLRIREQWKQNKNTLLENS----QRVTTAL 227

Query: 240 SASASSNKLPEELPQKALRL---CAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYY--T 299
            A +  +    +LP  A  +   C +QL + YD  +GGF  APKFP PV +  +  Y  +
Sbjct: 228 LARSEISVGDRQLPPSAATVNNRCFQQLDEGYDEEYGGFAEAPKFPTPVILSFLFSYWLS 287

Query: 300 KILEESGKSVEAEESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQ 359
             L + G   +      M +  L+ M+ GGI DHVG GFHRYS D +WHVPHFEKMLYDQ
Sbjct: 288 HRLTQDGSRAQ-----QMALHTLKMMANGGIRDHVGQGFHRYSTDRQWHVPHFEKMLYDQ 347

Query: 360 GQIANVYLDAFSITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKE 419
            Q+A  Y  AF ++ D  YS V++ +L Y+ R +    G  YSAEDADS    G  R KE
Sbjct: 348 AQLAVAYSQAFQLSGDEFYSDVAKGILQYVARSLSHRSGGFYSAEDADSPPERG-QRPKE 407

Query: 420 GAFYVWTSKEVDDTLGE----------HADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKN 479
           GA+YVWT KEV   L E                +HY +  +GN  +S   DP GE +G+N
Sbjct: 408 GAYYVWTVKEVQQLLPEPVLGATEPLTSGQLLMKHYGLTEAGN--ISPSQDPKGELQGQN 467

Query: 480 VLIEMKSVSEIASKHGLPVEKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISS 539
           VL    S+   A++ GL VE    +L     KLF+ R+HRPKPHLD K++ +WNGL +S 
Sbjct: 468 VLTVRYSLELTAARFGLDVEAVRTLLNSGLEKLFQARKHRPKPHLDSKMLAAWNGLMVSG 527

Query: 540 FARASKILRNEEDGTRFYFPVVGSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGP 599
           +A    +L              G D    +  A   A F++  ++D  + RL  +   GP
Sbjct: 528 YAVTGAVL--------------GQD--RLINYATNGAKFLKRHMFDVASGRLMRTCYTGP 587

Query: 600 ------SKAP--GFLDDYAFLIGGLLDLYEY-----------------------GEGGGY 659
                 S  P  GFL+DYAF++ GLLDLYE                         +GGGY
Sbjct: 588 GGTVEHSNPPCWGFLEDYAFVVRGLLDLYEASQESAWLEWALRLQDTQDKLFWDSQGGGY 647

Query: 660 YNTAGE-DPSILLRVKEDHDGAEPSGNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVF 719
           + +  E    + LR+K+D DGAEPS NSVSA NL+RL    +G K   +      LL  F
Sbjct: 648 FCSEAELGAGLPLRLKDDQDGAEPSANSVSAHNLLRLHGF-TGHKD--WMDKCVCLLTAF 707

Query: 720 EKRLKDTAVAVPLMCCAADMFSVPSRKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHI 779
            +R++   VA+P M  A       + K +V+ G + ++  + +V   H+ Y PN+ +I  
Sbjct: 708 SERMRRVPVALPEMVRALSA-QQQTLKQIVICGDRQAKDTKALVQCVHSVYIPNKVLILA 767

Query: 780 DTTDEMEMQFWEENNRNVAAMATNNFAADKVVGMVCQNFTCKAPVSDPESLEAML 782
           D      +      +R +  ++T     D+    VC+N  C  P++DP  L  +L
Sbjct: 768 DGDPSSFL------SRQLPFLSTLRRLEDQATAYVCENQACSVPITDPCELRKLL 784

BLAST of Cp4.1LG17g06880 vs. ExPASy Swiss-Prot
Match: Q6T393 (Spermatogenesis-associated protein 20 OS=Rattus norvegicus OX=10116 GN=Spata20 PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 2.5e-152
Identity = 303/763 (39.71%), Postives = 431/763 (56.49%), Query Frame = 0

Query: 66  KYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVMEVE 125
           K  N L  E SPYLLQHAHNPV+WYPWG EAF++A+K N PIFLS+GYSTCHWCH+ME E
Sbjct: 63  KTANRLINEKSPYLLQHAHNPVDWYPWGQEAFDKAKKENKPIFLSVGYSTCHWCHMMEEE 122

Query: 126 SFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMG 185
           SF+NEE+  LLN+ F+S+ VDREERPDVDKVYMT+VQA  SGGGWP++V+L+P L+P +G
Sbjct: 123 SFQNEEIGHLLNENFVSVMVDREERPDVDKVYMTFVQATSSGGGWPMNVWLTPSLQPFVG 182

Query: 186 GTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLPEE 245
           GTYFPP+D   R GF+TVL ++ D W   ++ L+++     ++++ AL A +  +    +
Sbjct: 183 GTYFPPEDGLTRVGFRTVLMRICDQWKQNKNTLLENS----QRVTTALLARSEISVGDRQ 242

Query: 246 LPQKALRL---CAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEE 305
           LP  A  +   C +QL + YD  +GGF  APKFP PV +  +  Y      +     A++
Sbjct: 243 LPPSAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPVILNFLFSYWLSHRVTQDGSRAQQ 302

Query: 306 SLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSIT 365
              M +  L+ M+ GGI DHVG GFHRYS D +WH+PHFEKMLYDQ Q++ VY  AF I+
Sbjct: 303 ---MALHTLKMMANGGIRDHVGQGFHRYSTDRQWHIPHFEKMLYDQAQLSVVYCQAFQIS 362

Query: 366 KDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDT 425
            D  +S V++ +L Y+ R++    G  YSAEDADS    G  + +EGA Y+WT KEV   
Sbjct: 363 GDEFFSDVAKGILQYVTRNLSHRSGGFYSAEDADSPPERG-VKPQEGALYLWTVKEVQQL 422

Query: 426 LGE----------HADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASK 485
           L E                +HY +  +GN  ++   D +GE  G+NVL    S+    ++
Sbjct: 423 LPEPVGGASEPLTSGQLLMKHYGLSEAGN--INPTQDVNGEMHGQNVLTVRDSLELTGAR 482

Query: 486 HGLPVEKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDG 545
           +GL VE    +L     KLF+ R+HRPK HLD+K++ +WNGL +S FA A  +L  E+  
Sbjct: 483 YGLEVEAVRALLNTGLEKLFQARKHRPKAHLDNKMLAAWNGLMVSGFAVAGSVLGMEKLV 542

Query: 546 TRFYFPVVGSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNG------PSKAP--GF 605
           T+                A   A F++  ++D  + RL+ +   G       S  P  GF
Sbjct: 543 TQ----------------ATNGAKFLKRHMFDVSSGRLKRTCYAGAGGTVEQSNPPCWGF 602

Query: 606 LDDYAFLIGGLLDLYEYGE-----------------------GGGYYNTAGE-DPSILLR 665
           L+DYAF++ GLLDLYE  +                       GGGY+ +  E    + LR
Sbjct: 603 LEDYAFVVRGLLDLYEASQESSWLEWALRLQDIQDKLFWDSHGGGYFCSEAELGTDLPLR 662

Query: 666 VKEDHDGAEPSGNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLM 725
           +K+D DGAEPS NSVSA NL+RL  L +G K   +      LL  F +R++   VA+P M
Sbjct: 663 LKDDQDGAEPSANSVSAHNLLRLHGL-TGHKD--WMDKCVCLLTAFSERMRRVPVALPEM 722

Query: 726 CCAADMFSVPSRKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEEN 784
             A       + K +V+ G   ++  + ++   H+ Y PN+ +I  D      +      
Sbjct: 723 VRALSA-QQQTLKQIVICGDPQAKDTKALLQCVHSIYIPNKVLILADGDPSSFL------ 782

BLAST of Cp4.1LG17g06880 vs. ExPASy Swiss-Prot
Match: Q80YT5 (Spermatogenesis-associated protein 20 OS=Mus musculus OX=10090 GN=Spata20 PE=1 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 3.7e-151
Identity = 303/765 (39.61%), Postives = 432/765 (56.47%), Query Frame = 0

Query: 66  KYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVMEVE 125
           K  N L  E SPYLLQHA+NPV+WYPWG EAF++A+K N PIFLS+GYSTCHWCH+ME E
Sbjct: 64  KTVNRLINEKSPYLLQHAYNPVDWYPWGQEAFDKAKKENKPIFLSVGYSTCHWCHMMEEE 123

Query: 126 SFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMG 185
           SF+NEE+ +LLN+ FI + VDREERPDVDKVYMT+VQA  SGGGWP++V+L+P L+P +G
Sbjct: 124 SFQNEEIGRLLNENFICVMVDREERPDVDKVYMTFVQATSSGGGWPMNVWLTPGLQPFVG 183

Query: 186 GTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLPEE 245
           GTYFPP+D   R GF+TVL ++ D W   ++ L+++     ++++ AL A +  +    +
Sbjct: 184 GTYFPPEDGLTRVGFRTVLMRICDQWKLNKNTLLENS----QRVTTALLARSEISVGDRQ 243

Query: 246 LPQKALRL---CAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYY--TKILEESGKSVEA 305
           +P  A  +   C +QL + YD  +GGF  APKFP PV +  +  Y  +  L + G   + 
Sbjct: 244 IPASAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPVILNFLFSYWLSHRLTQDGSRAQ- 303

Query: 306 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 365
                M +  L+ M+ GGI DHVG GFHRYS D +WH+PHFEKMLYDQ Q++ VY  AF 
Sbjct: 304 ----QMALHTLKMMANGGIQDHVGQGFHRYSTDRQWHIPHFEKMLYDQAQLSVVYTQAFQ 363

Query: 366 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 425
           I+ D  Y+ V++ +L Y+ R +    G  YSAEDADS    G  + +EGA+YVWT KEV 
Sbjct: 364 ISGDEFYADVAKGILQYVTRTLSHRSGGFYSAEDADSPPERG-MKPQEGAYYVWTVKEVQ 423

Query: 426 DTLGE----------HADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIA 485
             L E                +HY +   GN + S+  DP+GE  G+NVL+   S+   A
Sbjct: 424 QLLPEPVVGASEPLTSGQLLMKHYGLSEVGNINSSQ--DPNGELHGQNVLMVRYSLELTA 483

Query: 486 SKHGLPVEKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEE 545
           +++GL VE    +L     KLF+ R+HRPK HLD+K++ +WNGL +S FA     L  E 
Sbjct: 484 ARYGLEVEAVRALLNTGLEKLFQARKHRPKAHLDNKMLAAWNGLMVSGFAVTGAALGME- 543

Query: 546 DGTRFYFPVVGSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNG------PSKAP-- 605
                          + +  A   A F++  ++D  + RL+ +   G       S  P  
Sbjct: 544 ---------------KLVAQATSGAKFLKRHMFDVSSGRLKRTCYAGTGGTVEQSNPPCW 603

Query: 606 GFLDDYAFLIGGLLDLYEYGE-----------------------GGGYYNTAGE-DPSIL 665
           GFL+DYAF++ GLLDLYE  +                       GGGY+ +  E    + 
Sbjct: 604 GFLEDYAFVVRGLLDLYEASQESSWLEWALRLQDTQDKLFWDPRGGGYFCSEAELGADLP 663

Query: 666 LRVKEDHDGAEPSGNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVP 725
           LR+K+D DGAEPS NSVSA NL+RL S  +G K   +      LL  F +R++   VA+P
Sbjct: 664 LRLKDDQDGAEPSANSVSAHNLLRLHSF-TGHKD--WMDKCVCLLTAFSERMRRVPVALP 723

Query: 726 LMCCAADMFSVPSRKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWE 784
            M          + K +V+ G   ++  + ++   H+ Y PN+ +I  D      +    
Sbjct: 724 EMVRTLSA-QQQTLKQIVICGDPQAKDTKALLQCVHSIYVPNKVLILADGDPSSFL---- 783

BLAST of Cp4.1LG17g06880 vs. ExPASy Swiss-Prot
Match: Q09214 (Uncharacterized protein B0495.5 OS=Caenorhabditis elegans OX=6239 GN=B0495.5 PE=4 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 6.5e-140
Identity = 285/750 (38.00%), Postives = 408/750 (54.40%), Query Frame = 0

Query: 67  YTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVMEVES 126
           Y N L  E SPYLLQHA+NP++WYPWG EAF++A+  N PIFLS+GYSTCHWCHVME ES
Sbjct: 17  YKNRLGQEKSPYLLQHANNPIDWYPWGQEAFQKAKDNNKPIFLSVGYSTCHWCHVMEKES 76

Query: 127 FENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMGG 186
           FENE  AK+LND F++IKVDREERPDVDK+YM +V A    GGWP+SVFL+PDL P+ GG
Sbjct: 77  FENEATAKILNDNFVAIKVDREERPDVDKLYMAFVVASSGHGGWPMSVFLTPDLHPITGG 136

Query: 187 TYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLPEEL 246
           TYFPPDD  G  GF T+L  +   W  + + L + G   I +L +  +AS   N+     
Sbjct: 137 TYFPPDDNRGMLGFPTILNMIHTEWKKEGESLKQRGAQII-KLLQPETASGDVNR----- 196

Query: 247 PQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEESLNM 306
            ++  +        S+D   GGFG APKFP+  ++  ++ +     ES K   A++S+ M
Sbjct: 197 SEEVFKSIYSHKQSSFDSRLGGFGRAPKFPKACDLDFLITFAASENESEK---AKDSIMM 256

Query: 307 VIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSIT--KD 366
           +   L+ M+ GGIHDH+G GFHRYSV   WH+PHFEKMLYDQ Q+   Y D   +T  K 
Sbjct: 257 LQKTLESMADGGIHDHIGNGFHRYSVGSEWHIPHFEKMLYDQSQLLATYSDFHKLTERKH 316

Query: 367 VSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDTLG 426
            +   V  D+  Y+++ +   +G  Y+AEDADS  +  ++ K EGAF  W  +E+   LG
Sbjct: 317 DNVKHVINDIYQYMQK-ISHKDGGFYAAEDADSLPNHNSSNKVEGAFCAWEKEEIKQLLG 376

Query: 427 EHA-------DFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPV 486
           +         D   +++ ++ SGN  ++R SDPHGE K KNVL ++ +  E A+ H + V
Sbjct: 377 DKKIGSASLFDVVADYFDVEDSGN--VARSSDPHGELKNKNVLRKLLTDEECATNHEISV 436

Query: 487 EKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYF 546
            +  + + E +  L+  R  RP PHLD K++ SW GL I+   +A +             
Sbjct: 437 AELKKGIDEAKEILWNARTQRPSPHLDSKMVTSWQGLAITGLVKAYQ------------- 496

Query: 547 PVVGSDAKEYLKVAEKAALFIRTKLYDE-QTRRLQHSFRNGP-----SKAPGFLDDYAFL 606
               ++  +YL  AEK A FI   L D  + RR  +   NG       +   F DDYAFL
Sbjct: 497 ---ATEETKYLDRAEKCAEFIGKFLDDNGELRRSVYLGANGEVEQGNQEIRAFSDDYAFL 556

Query: 607 IGGLLDLY---------------------EYGEGGGYYNTAGEDPSILLRVKEDHDGAEP 666
           I  LLDLY                     ++  G GY+ +   D  + +R+ ED DGAEP
Sbjct: 557 IQALLDLYTTVGKDEYLKKAVELQKICDVKFWNGNGYFISEKTDEDVSVRMIEDQDGAEP 616

Query: 667 SGNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVP 726
           +  S+++ NL+RL  ++   + + YR+ A        +RL    +A+P M  A   + + 
Sbjct: 617 TATSIASNNLLRLYDIL---EKEEYREKANQCFRGASERLNTVPIALPKMAVALHRWQIG 676

Query: 727 SRKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATN 781
           S    VLVG   SE      +  +  +  N +V+HI + +++       +  +  AMA  
Sbjct: 677 STT-FVLVGDPKSELLSETRSRLNQKFLNNLSVVHIQSEEDL-----SASGPSHKAMA-- 725

BLAST of Cp4.1LG17g06880 vs. ExPASy Swiss-Prot
Match: P37512 (Uncharacterized protein YyaL OS=Bacillus subtilis (strain 168) OX=224308 GN=yyaL PE=4 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 5.2e-129
Identity = 267/734 (36.38%), Postives = 400/734 (54.50%), Query Frame = 0

Query: 69  NLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVMEVESFE 128
           N L  E SPYLLQHAHNPV+W+PWG+EAFE+A++ N P+ +SIGYSTCHWCHVM  ESFE
Sbjct: 8   NRLINEKSPYLLQHAHNPVDWFPWGEEAFEKAKRENKPVLVSIGYSTCHWCHVMAHESFE 67

Query: 129 NEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMGGTY 188
           +EE+A+LLN+ F++IKVDREERPDVD VYM   Q +   GGWPL+VF++PD KP   GTY
Sbjct: 68  DEEIARLLNERFVAIKVDREERPDVDSVYMRICQLMTGQGGWPLNVFITPDQKPFYAGTY 127

Query: 189 FPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLPEELPQ 248
           FP   K+ RPGF  VL  + + + N R+ +      A + L    +A     K  E L +
Sbjct: 128 FPKTSKFNRPGFVDVLEHLSETFANDREHVEDIAENAAKHLQTKTAA-----KTGEGLSE 187

Query: 249 KALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEESLNMVI 308
            A+    +QL+  +D  +GGFG APKFP P    +++Y  +    +G+    E +L  V 
Sbjct: 188 SAIHRTFQQLASGFDTIYGGFGQAPKFPMP---HMLMYLLRYDHNTGQ----ENALYNVT 247

Query: 309 FGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSITKDVSYS 368
             L  M+ GGI+DH+G GF RYS D+ W VPHFEKMLYD   +   Y +A+ +T++  Y 
Sbjct: 248 KTLDSMANGGIYDHIGYGFARYSTDDEWLVPHFEKMLYDNALLLTAYTEAYQVTQNSRYK 307

Query: 369 CVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDTLGEH-A 428
            +   ++ +++R+M   +G  +SA DAD       T  +EG +YVW+ +E+  TLG+   
Sbjct: 308 EICEQIITFIQREMTHEDGSFFSALDAD-------TEGEEGKYYVWSKEEILKTLGDDLG 367

Query: 429 DFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNV--LIEMKSVSEIASKHGLPVEKYLEILG 488
             + + Y I   GN            F+GKN+  LI  K   +I    GL  ++    L 
Sbjct: 368 TLYCQVYDITEEGN------------FEGKNIPNLIHTKR-EQIKEDAGLTEKELSLKLE 427

Query: 489 ECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVVGSDAK 548
           + R +L + RE R  PH+DDKV+ SWN L I+  A+A+K+ +                  
Sbjct: 428 DARQQLLKTREERTYPHVDDKVLTSWNALMIAGLAKAAKVYQE----------------P 487

Query: 549 EYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYG--- 608
           +YL +A+ A  FI  KL  +   R+   +R+G  K  GF+DDYAFL+   LDLYE     
Sbjct: 488 KYLSLAKDAITFIENKLIID--GRVMVRYRDGEVKNKGFIDDYAFLLWAYLDLYEASFDL 547

Query: 609 --------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSAINLVR 668
                               E GG+Y T  +  ++++R KE +DGA PSGNSV+A+ L+R
Sbjct: 548 SYLQKAKKLTDDMISLFWDEEHGGFYFTGHDAEALIVREKEVYDGAVPSGNSVAAVQLLR 607

Query: 669 LSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVLVGHKN 728
           L   V+G  S    + AE + +VF+  ++           +     +P +K +V+ G  +
Sbjct: 608 LGQ-VTGDLS--LIEKAETMFSVFKPDIEAYPSGHAFFMQSVLRHLMP-KKEIVIFGSAD 667

Query: 729 SEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAAD-KVVGMV 776
               + ++A    ++ PN +++  +  ++          +++A  A +    D K    +
Sbjct: 668 DPARKQIIAELQKAFKPNDSILVAEQPEQC---------KDIAPFAADYRIIDGKTTVYI 678

BLAST of Cp4.1LG17g06880 vs. NCBI nr
Match: XP_023514060.1 (spermatogenesis-associated protein 20 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1593 bits (4126), Expect = 0.0
Identity = 784/809 (96.91%), Postives = 785/809 (97.03%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG
Sbjct: 39  LRKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 98

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
           GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH
Sbjct: 99  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 158

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 159 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 218

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN
Sbjct: 219 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 278

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA
Sbjct: 279 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 338

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 339 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 398

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD
Sbjct: 399 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 458

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 459 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 518

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV
Sbjct: 519 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 578

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 579 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 638

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 639 YGGGLNWLIWAIELQATQDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 698

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL
Sbjct: 699 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 758

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV
Sbjct: 759 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 818

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VGMVCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 819 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 847

BLAST of Cp4.1LG17g06880 vs. NCBI nr
Match: XP_022959785.1 (spermatogenesis-associated protein 20 [Cucurbita moschata])

HSP 1 Score: 1549 bits (4010), Expect = 0.0
Identity = 763/809 (94.31%), Postives = 772/809 (95.43%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRF RRRYPSEFSPNSTPIPFPRFPFLLSHSP RFRAPFHACKVLAMAAQSSDG
Sbjct: 39  LRKLPLRRFLRRRYPSEFSPNSTPIPFPRFPFLLSHSPFRFRAPFHACKVLAMAAQSSDG 98

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
           GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH
Sbjct: 99  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 158

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 159 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 218

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN
Sbjct: 219 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 278

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEELPQ ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTK LEESGKS EA
Sbjct: 279 KLPEELPQNALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKKLEESGKSGEA 338

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIF LQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 339 EESLNMVIFSLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 398

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYS VSRDVLDYLRRDMIGP+GEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD
Sbjct: 399 ITKDVSYSYVSRDVLDYLRRDMIGPDGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 458

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           +TLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 459 ETLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 518

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECR KLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNE++GTRF FPVV
Sbjct: 519 LEILGECRQKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEKEGTRFNFPVV 578

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GSDAKEY KVAEKAALFIRTKLY+EQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 579 GSDAKEYFKVAEKAALFIRTKLYNEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 638

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 639 YGGGLNWLIWAIELQATLDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 698

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL
Sbjct: 699 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 758

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKN EQFETM+AAAHASYDPNRTVIHIDTTD+MEMQFWEENNRNV AMA NNFAADKV
Sbjct: 759 VGHKNWEQFETMLAAAHASYDPNRTVIHIDTTDKMEMQFWEENNRNVGAMAKNNFAADKV 818

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VG+VCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 819 VGLVCQNFTCKAPVSDPESLEAMLAQKPS 847

BLAST of Cp4.1LG17g06880 vs. NCBI nr
Match: XP_023004454.1 (spermatogenesis-associated protein 20 [Cucurbita maxima])

HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 759/809 (93.82%), Postives = 766/809 (94.68%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRF RR YPSE SPNSTPIPFPRFPFL SHSP RF APFHACKVLAMAAQSSDG
Sbjct: 38  LRKLPLRRFLRRCYPSELSPNSTPIPFPRFPFLPSHSPFRFCAPFHACKVLAMAAQSSDG 97

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
             HSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH
Sbjct: 98  SPHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 157

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 158 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 217

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASS 
Sbjct: 218 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSK 277

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEEL Q AL LCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTK LEESGKS EA
Sbjct: 278 KLPEELQQNALLLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKKLEESGKSGEA 337

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 338 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 397

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYSC+SRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD
Sbjct: 398 ITKDVSYSCISRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 457

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 458 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 517

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECR KLFRVREHRPKP+LDDKVIVSWNGLTISSFARASKILRNE+DGTRFYFPVV
Sbjct: 518 LEILGECRQKLFRVREHRPKPYLDDKVIVSWNGLTISSFARASKILRNEKDGTRFYFPVV 577

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GSDAKEY KVAEKAALFIRTKLYDEQT RLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 578 GSDAKEYFKVAEKAALFIRTKLYDEQTHRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 637

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 638 YGGGLNWLIWAIELQATQDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 697

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMF VPSRKHVVL
Sbjct: 698 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFLVPSRKHVVL 757

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKNSEQFETM+AAAHASYDPNRTVIHIDTTDE+EMQFWEENNRNVAAMA NNFAADKV
Sbjct: 758 VGHKNSEQFETMLAAAHASYDPNRTVIHIDTTDEIEMQFWEENNRNVAAMAKNNFAADKV 817

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VG+VCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 818 VGLVCQNFTCKAPVSDPESLEAMLAQKPS 846

BLAST of Cp4.1LG17g06880 vs. NCBI nr
Match: KAG6593178.1 (Spermatogenesis-associated protein 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1485 bits (3845), Expect = 0.0
Identity = 739/809 (91.35%), Postives = 751/809 (92.83%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRF RRRYPSEFSPNSTPIPFPRFPFL SHSP RF APFHACKVLAMAAQSSDG
Sbjct: 39  LRKLPLRRFLRRRYPSEFSPNSTPIPFPRFPFLPSHSPFRFCAPFHACKVLAMAAQSSDG 98

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
           GSHSHKYTNLLAVE SPYLLQHAHNP+           +           +GYSTCHWCH
Sbjct: 99  GSHSHKYTNLLAVEQSPYLLQHAHNPL---------IHDEIDSLFFFSFEVGYSTCHWCH 158

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 159 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 218

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN
Sbjct: 219 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 278

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEELPQ ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTK LEESGKSVEA
Sbjct: 279 KLPEELPQNALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKKLEESGKSVEA 338

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIF LQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 339 EESLNMVIFSLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 398

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGA+YVWTSKEVD
Sbjct: 399 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAYYVWTSKEVD 458

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           +TLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 459 ETLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 518

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECR KLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNE+DGTRFYFPVV
Sbjct: 519 LEILGECRQKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEKDGTRFYFPVV 578

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GS+AKEY KVAEKAALFIRTKL+DEQTRRLQHSFRN PSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 579 GSNAKEYFKVAEKAALFIRTKLFDEQTRRLQHSFRNRPSKAPGFLDDYAFLIGGLLDLYE 638

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 639 YGGGLNWLIWAIELQATQDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 698

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL
Sbjct: 699 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 758

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKNSEQFETM+AAAHASYDPNRTVIHIDTT+EMEMQFWEENNRNVAAMA NNFAADKV
Sbjct: 759 VGHKNSEQFETMLAAAHASYDPNRTVIHIDTTNEMEMQFWEENNRNVAAMAKNNFAADKV 818

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VG+VCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 819 VGLVCQNFTCKAPVSDPESLEAMLAQKPS 838

BLAST of Cp4.1LG17g06880 vs. NCBI nr
Match: XP_038876725.1 (spermatogenesis-associated protein 20 [Benincasa hispida])

HSP 1 Score: 1411 bits (3653), Expect = 0.0
Identity = 690/806 (85.61%), Postives = 728/806 (90.32%), Query Frame = 0

Query: 4   LPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDGGSH 63
           L LRRF R   PS+ SP S  +PFPRFPF  S    R   P +  K+LAMAAQSS G +H
Sbjct: 5   LSLRRFLRHCNPSDSSPKSPSLPFPRFPFPSSPFSFRLSTPIYPHKLLAMAAQSSGGSAH 64

Query: 64  SHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVME 123
            H YTN LA EHSPYLLQHAHNPVNWYPWG+EAF EA+KRNVPIFLSIGYSTCHWCHVME
Sbjct: 65  PHNYTNHLATEHSPYLLQHAHNPVNWYPWGEEAFAEAQKRNVPIFLSIGYSTCHWCHVME 124

Query: 124 VESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPL 183
           VESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPL
Sbjct: 125 VESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPL 184

Query: 184 MGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLP 243
           MGGTYFPPDDKYGRPGFKTVLRKVK+AWDNKRDVLVKSGT+AIEQLSEALSA+ASSNKLP
Sbjct: 185 MGGTYFPPDDKYGRPGFKTVLRKVKEAWDNKRDVLVKSGTFAIEQLSEALSATASSNKLP 244

Query: 244 EELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEES 303
           EELPQ ALRLCA QLSQSYDPNFGGFGS+PKFPRPVEVQLMLYY K LEESGKS EAEES
Sbjct: 245 EELPQNALRLCAVQLSQSYDPNFGGFGSSPKFPRPVEVQLMLYYAKKLEESGKSDEAEES 304

Query: 304 LNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSITK 363
           LNMV FGLQCM+RGGIHDHVGGGFHRYSVDE WHVPHFEKMLYDQGQI NVYLDAFSITK
Sbjct: 305 LNMVTFGLQCMARGGIHDHVGGGFHRYSVDECWHVPHFEKMLYDQGQITNVYLDAFSITK 364

Query: 364 DVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDTL 423
           D SYSCVSRDVLDYLRR+MIGP+GEI+SAEDADSAESEGATRKKEGAFYVWTSKE+DD L
Sbjct: 365 DASYSCVSRDVLDYLRRNMIGPQGEIFSAEDADSAESEGATRKKEGAFYVWTSKELDDIL 424

Query: 424 GEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKYLEI 483
           GEHADFF++HYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSE+ASKHG+PVEKYLEI
Sbjct: 425 GEHADFFRDHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEMASKHGMPVEKYLEI 484

Query: 484 LGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVVGSD 543
           LGECR KLF+VREHRPKPHLDDKVIVSWNGLTISSFARASKIL NE++GTRFYFPVVG D
Sbjct: 485 LGECRQKLFKVREHRPKPHLDDKVIVSWNGLTISSFARASKILGNEKEGTRFYFPVVGCD 544

Query: 544 AKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYG- 603
           AKEY +VAEKAALFIRTKLYDEQT RLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYG 
Sbjct: 545 AKEYFEVAEKAALFIRTKLYDEQTHRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYGG 604

Query: 604 ----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSAINL 663
                                 EGGGYYNT GED S+LLRVKEDHDGAEPSGNSVSAINL
Sbjct: 605 GLNWLVWAIELQATQDELFLDREGGGYYNTTGEDASVLLRVKEDHDGAEPSGNSVSAINL 664

Query: 664 VRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVLVGH 723
           +RLSSLVSGS+S++YRQNAEHLLAVFEKRLKD AVAVPLMCCAADMFS+PSRK VVLVGH
Sbjct: 665 IRLSSLVSGSRSNHYRQNAEHLLAVFEKRLKDMAVAVPLMCCAADMFSIPSRKQVVLVGH 724

Query: 724 KNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKVVGM 783
           KNS QFET++AAAHASYDPNRTVIHID TD+ E+QFWE NNRNVA MA NNFAADKVV +
Sbjct: 725 KNSAQFETILAAAHASYDPNRTVIHIDPTDDTELQFWEGNNRNVAVMAKNNFAADKVVAL 784

Query: 784 VCQNFTCKAPVSDPESLEAMLAQKPS 786
           +CQNFTCKAP++DP SLEAMLAQKPS
Sbjct: 785 ICQNFTCKAPITDPGSLEAMLAQKPS 810

BLAST of Cp4.1LG17g06880 vs. ExPASy TrEMBL
Match: A0A6J1H7B1 (spermatogenesis-associated protein 20 OS=Cucurbita moschata OX=3662 GN=LOC111460750 PE=4 SV=1)

HSP 1 Score: 1549 bits (4010), Expect = 0.0
Identity = 763/809 (94.31%), Postives = 772/809 (95.43%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRF RRRYPSEFSPNSTPIPFPRFPFLLSHSP RFRAPFHACKVLAMAAQSSDG
Sbjct: 39  LRKLPLRRFLRRRYPSEFSPNSTPIPFPRFPFLLSHSPFRFRAPFHACKVLAMAAQSSDG 98

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
           GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH
Sbjct: 99  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 158

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 159 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 218

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN
Sbjct: 219 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 278

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEELPQ ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTK LEESGKS EA
Sbjct: 279 KLPEELPQNALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKKLEESGKSGEA 338

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIF LQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 339 EESLNMVIFSLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 398

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYS VSRDVLDYLRRDMIGP+GEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD
Sbjct: 399 ITKDVSYSYVSRDVLDYLRRDMIGPDGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 458

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           +TLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 459 ETLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 518

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECR KLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNE++GTRF FPVV
Sbjct: 519 LEILGECRQKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEKEGTRFNFPVV 578

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GSDAKEY KVAEKAALFIRTKLY+EQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 579 GSDAKEYFKVAEKAALFIRTKLYNEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 638

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 639 YGGGLNWLIWAIELQATLDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 698

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL
Sbjct: 699 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 758

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKN EQFETM+AAAHASYDPNRTVIHIDTTD+MEMQFWEENNRNV AMA NNFAADKV
Sbjct: 759 VGHKNWEQFETMLAAAHASYDPNRTVIHIDTTDKMEMQFWEENNRNVGAMAKNNFAADKV 818

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VG+VCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 819 VGLVCQNFTCKAPVSDPESLEAMLAQKPS 847

BLAST of Cp4.1LG17g06880 vs. ExPASy TrEMBL
Match: A0A6J1KWB0 (spermatogenesis-associated protein 20 OS=Cucurbita maxima OX=3661 GN=LOC111497758 PE=4 SV=1)

HSP 1 Score: 1539 bits (3984), Expect = 0.0
Identity = 759/809 (93.82%), Postives = 766/809 (94.68%), Query Frame = 0

Query: 1   MAKLPLRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG 60
           + KLPLRRF RR YPSE SPNSTPIPFPRFPFL SHSP RF APFHACKVLAMAAQSSDG
Sbjct: 38  LRKLPLRRFLRRCYPSELSPNSTPIPFPRFPFLPSHSPFRFCAPFHACKVLAMAAQSSDG 97

Query: 61  GSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 120
             HSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH
Sbjct: 98  SPHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCH 157

Query: 121 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 180
           VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL
Sbjct: 158 VMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDL 217

Query: 181 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSN 240
           KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASS 
Sbjct: 218 KPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSK 277

Query: 241 KLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEA 300
           KLPEEL Q AL LCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTK LEESGKS EA
Sbjct: 278 KLPEELQQNALLLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKKLEESGKSGEA 337

Query: 301 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 360
           EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS
Sbjct: 338 EESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFS 397

Query: 361 ITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 420
           ITKDVSYSC+SRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD
Sbjct: 398 ITKDVSYSCISRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVD 457

Query: 421 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 480
           DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY
Sbjct: 458 DTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKY 517

Query: 481 LEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVV 540
           LEILGECR KLFRVREHRPKP+LDDKVIVSWNGLTISSFARASKILRNE+DGTRFYFPVV
Sbjct: 518 LEILGECRQKLFRVREHRPKPYLDDKVIVSWNGLTISSFARASKILRNEKDGTRFYFPVV 577

Query: 541 GSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 600
           GSDAKEY KVAEKAALFIRTKLYDEQT RLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE
Sbjct: 578 GSDAKEYFKVAEKAALFIRTKLYDEQTHRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYE 637

Query: 601 YG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 660
           YG                       EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA
Sbjct: 638 YGGGLNWLIWAIELQATQDELFLDREGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSA 697

Query: 661 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVL 720
           INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMF VPSRKHVVL
Sbjct: 698 INLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFLVPSRKHVVL 757

Query: 721 VGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKV 780
           VGHKNSEQFETM+AAAHASYDPNRTVIHIDTTDE+EMQFWEENNRNVAAMA NNFAADKV
Sbjct: 758 VGHKNSEQFETMLAAAHASYDPNRTVIHIDTTDEIEMQFWEENNRNVAAMAKNNFAADKV 817

Query: 781 VGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           VG+VCQNFTCKAPVSDPESLEAMLAQKPS
Sbjct: 818 VGLVCQNFTCKAPVSDPESLEAMLAQKPS 846

BLAST of Cp4.1LG17g06880 vs. ExPASy TrEMBL
Match: A0A1S3BRB9 (spermatogenesis-associated protein 20 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103492853 PE=4 SV=1)

HSP 1 Score: 1379 bits (3568), Expect = 0.0
Identity = 676/801 (84.39%), Postives = 711/801 (88.76%), Query Frame = 0

Query: 9   FPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDGGSHSHKYT 68
           F  R + S  SP+   +PF RFPFL S    RF  P +  KV AMAA+SS G SHSH YT
Sbjct: 58  FSLRHFNSSISPS---LPFQRFPFLSSPFSFRFSTPIYPHKVFAMAARSSGGSSHSHSYT 117

Query: 69  NLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVMEVESFE 128
           N LA EHSPYLLQHAHNPVNWYPWG+EAF EA+KRNVPIFLSIGYSTCHWCHVMEVESFE
Sbjct: 118 NRLATEHSPYLLQHAHNPVNWYPWGEEAFAEAQKRNVPIFLSIGYSTCHWCHVMEVESFE 177

Query: 129 NEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMGGTY 188
           N+EVAKLLNDWF+SIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMGGTY
Sbjct: 178 NKEVAKLLNDWFVSIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPLMGGTY 237

Query: 189 FPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLPEELPQ 248
           FPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGT+AIEQLSEALS +ASSNKLPEELPQ
Sbjct: 238 FPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTFAIEQLSEALSTTASSNKLPEELPQ 297

Query: 249 KALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEESLNMVI 308
            ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVE QLMLYY K LEESGKS EAEE LNMV 
Sbjct: 298 NALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEAQLMLYYAKKLEESGKSDEAEEILNMVF 357

Query: 309 FGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSITKDVSYS 368
           FGLQCM+RGGIHDHVGGGFHRYSVDE WHVPHFEKMLYDQGQI NVYLDAFSITKDVSYS
Sbjct: 358 FGLQCMARGGIHDHVGGGFHRYSVDECWHVPHFEKMLYDQGQITNVYLDAFSITKDVSYS 417

Query: 369 CVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDTLGEHAD 428
            VSRD+LDYLRRDMIG +GEI+SAEDADSAESEGATRKKEGAFYVWTSKE+DD LGEHAD
Sbjct: 418 WVSRDILDYLRRDMIGTQGEIFSAEDADSAESEGATRKKEGAFYVWTSKEIDDILGEHAD 477

Query: 429 FFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKYLEILGECR 488
           FFKEHYYIKPSGNCDLSR+SDPH EFKGKNVLIEMKSVSE+AS HG+PVEKYLEILGECR
Sbjct: 478 FFKEHYYIKPSGNCDLSRMSDPHDEFKGKNVLIEMKSVSEMASNHGMPVEKYLEILGECR 537

Query: 489 LKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVVGSDAKEYL 548
            KLF+VRE RPKPHLDDKVIVSWNGLTISS ARASKILRNE++GTRFYFPVVG D KEYL
Sbjct: 538 QKLFKVRERRPKPHLDDKVIVSWNGLTISSLARASKILRNEKEGTRFYFPVVGCDPKEYL 597

Query: 549 KVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYG------ 608
            VAEKAALFI+TKLYDEQT RLQHSFRN PS+APGFLDDYAFLIGGLLDLYEYG      
Sbjct: 598 DVAEKAALFIKTKLYDEQTHRLQHSFRNAPSRAPGFLDDYAFLIGGLLDLYEYGGGLNWL 657

Query: 609 -----------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSAINLVRLSS 668
                            EGGGYYNT GED S++LRVKEDHDGAEPSGNSVSAINLVRLSS
Sbjct: 658 VWAIELQATQDELFLDREGGGYYNTTGEDKSVILRVKEDHDGAEPSGNSVSAINLVRLSS 717

Query: 669 LVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVLVGHKNSEQ 728
           LVSGS+S+YYRQNAEHLLAVFEKRLKD AVAVPLMCCAA M S PSRK VVLVGHKNS Q
Sbjct: 718 LVSGSRSNYYRQNAEHLLAVFEKRLKDMAVAVPLMCCAAGMLSTPSRKQVVLVGHKNSAQ 777

Query: 729 FETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKVVGMVCQNF 786
           FET +AAAHASYDPN TVIHID TD+ E+QFWEENNR VA MA NNFAADKVV +VCQNF
Sbjct: 778 FETFLAAAHASYDPNSTVIHIDPTDDTELQFWEENNRGVAVMAKNNFAADKVVALVCQNF 837

BLAST of Cp4.1LG17g06880 vs. ExPASy TrEMBL
Match: A0A6J1D1Z4 (spermatogenesis-associated protein 20 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016275 PE=4 SV=1)

HSP 1 Score: 1368 bits (3542), Expect = 0.0
Identity = 680/815 (83.44%), Postives = 717/815 (87.98%), Query Frame = 0

Query: 1   MAKLPL----RRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQ 60
           + KLPL     RF RR  PS+  P + P PF            RF  P        MAA 
Sbjct: 2   LTKLPLSSSLHRFLRRPTPSKIPPITRPFPFTD----------RFCTP--------MAAH 61

Query: 61  SSDGGS--HSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYS 120
           SS   S  HSHKY+N LA EHSPYLLQHAHNPVNWYPWG+EAF EARKRNVPIFLSIGYS
Sbjct: 62  SSHPASTSHSHKYSNRLAAEHSPYLLQHAHNPVNWYPWGEEAFVEARKRNVPIFLSIGYS 121

Query: 121 TCHWCHVMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSV 180
           TCHWCHVMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSV
Sbjct: 122 TCHWCHVMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSV 181

Query: 181 FLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALS 240
           FLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWD+KRDVLVKSGT+AIEQLSEALS
Sbjct: 182 FLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDDKRDVLVKSGTFAIEQLSEALS 241

Query: 241 ASASSNKLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEES 300
           A+ASSNKL  ELPQ ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYY K LEES
Sbjct: 242 ATASSNKLSTELPQNALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYIKKLEES 301

Query: 301 GKSVEAEESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANV 360
           GKS ++EE LNMV FGL+CM+RGGIHDHVGGGFHRYSVDE WHVPHFEKMLYDQGQI NV
Sbjct: 302 GKSGDSEEHLNMVSFGLKCMARGGIHDHVGGGFHRYSVDECWHVPHFEKMLYDQGQITNV 361

Query: 361 YLDAFSITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVW 420
           YLDAFSITKDV YSC+SRD+LDYLRRDMIGPEGEI+SAEDADSAESEGA R KEGAFYVW
Sbjct: 362 YLDAFSITKDVLYSCISRDILDYLRRDMIGPEGEIFSAEDADSAESEGAIRNKEGAFYVW 421

Query: 421 TSKEVDDTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHG 480
           TSKEVDD LGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEM S SE+ASKHG
Sbjct: 422 TSKEVDDILGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMTSTSEMASKHG 481

Query: 481 LPVEKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTR 540
           +PVEKYLEILGECR KLF+VRE RPKPHLDDKVIVSWNGLTISSFARASKILR+E++GTR
Sbjct: 482 IPVEKYLEILGECREKLFKVRESRPKPHLDDKVIVSWNGLTISSFARASKILRHEKEGTR 541

Query: 541 FYFPVVGSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGG 600
           FYFPVVG D KEY +VAEKAALFIRTKLY+EQT RLQHSFRNGPSKAPGFLDDYAFLIGG
Sbjct: 542 FYFPVVGCDVKEYFEVAEKAALFIRTKLYNEQTHRLQHSFRNGPSKAPGFLDDYAFLIGG 601

Query: 601 LLDLYEYG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPS 660
           LLDLYEYG                       EGGGYYNTAGEDPS+LLRVKEDHDGAEPS
Sbjct: 602 LLDLYEYGGGLNWLIWAIELQATQDELFLDREGGGYYNTAGEDPSVLLRVKEDHDGAEPS 661

Query: 661 GNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPS 720
           GNSVSAINLVRLSS+VSGS+SD+YRQNAEHLLAVFEKRLKD A+AVPLMCCAA+MFSVPS
Sbjct: 662 GNSVSAINLVRLSSMVSGSRSDHYRQNAEHLLAVFEKRLKDMAMAVPLMCCAANMFSVPS 721

Query: 721 RKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNN 780
           RKHVVL+GHK S QFETM+AA HASYDPNRTVIH+D TDE EM+FWEENN N+AAMA NN
Sbjct: 722 RKHVVLIGHKKSAQFETMLAAVHASYDPNRTVIHVDPTDETEMRFWEENNSNIAAMAKNN 781

Query: 781 FAADKVVGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           FAADKVVG+VCQNFTCKAPV+DPESL+AMLAQKPS
Sbjct: 782 FAADKVVGLVCQNFTCKAPVTDPESLKAMLAQKPS 798

BLAST of Cp4.1LG17g06880 vs. ExPASy TrEMBL
Match: A0A5D3D2G6 (Spermatogenesis-associated protein 20 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00130 PE=4 SV=1)

HSP 1 Score: 1352 bits (3500), Expect = 0.0
Identity = 658/757 (86.92%), Postives = 689/757 (91.02%), Query Frame = 0

Query: 53  MAAQSSDGGSHSHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIG 112
           MAA+SS G SHSH YTN LA EHSPYLLQHAHNPVNWYPWG+EAF EA+KRNVPIFLSIG
Sbjct: 1   MAARSSGGSSHSHSYTNRLATEHSPYLLQHAHNPVNWYPWGEEAFAEAQKRNVPIFLSIG 60

Query: 113 YSTCHWCHVMEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPL 172
           YSTCHWCHVMEVESFEN+EVAKLLNDWF+SIKVDREERPDVDKVYMTYVQALYSGGGWPL
Sbjct: 61  YSTCHWCHVMEVESFENKEVAKLLNDWFVSIKVDREERPDVDKVYMTYVQALYSGGGWPL 120

Query: 173 SVFLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEA 232
           SVFLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGT+AIEQLSEA
Sbjct: 121 SVFLSPDLKPLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTFAIEQLSEA 180

Query: 233 LSASASSNKLPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILE 292
           LS +ASSNKLPEELPQ ALRLCAEQLSQSYDPNFGGFGSAPKFPRPVE QLMLYY K LE
Sbjct: 181 LSTTASSNKLPEELPQNALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEAQLMLYYAKKLE 240

Query: 293 ESGKSVEAEESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIA 352
           ESGKS EAEE LNMV FGLQCM+RGGIHDHVGGGFHRYSVDE WHVPHFEKMLYDQGQI 
Sbjct: 241 ESGKSDEAEEILNMVFFGLQCMARGGIHDHVGGGFHRYSVDECWHVPHFEKMLYDQGQIT 300

Query: 353 NVYLDAFSITKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFY 412
           NVYLDAFSITKDVSYS VSRD+LDYLRRDMIG +GEI+SAEDADSAESEGATRKKEGAFY
Sbjct: 301 NVYLDAFSITKDVSYSWVSRDILDYLRRDMIGTQGEIFSAEDADSAESEGATRKKEGAFY 360

Query: 413 VWTSKEVDDTLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASK 472
           VWTSKE+DD LGEHADFFKEHYYIKPSGNCDLSR+SDPH EFKGKNVLIEMKSVSE+AS 
Sbjct: 361 VWTSKEIDDILGEHADFFKEHYYIKPSGNCDLSRMSDPHDEFKGKNVLIEMKSVSEMASN 420

Query: 473 HGLPVEKYLEILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDG 532
           HG+PVEKYLEILGECR KLF+VRE RPKPHLDDKVIVSWNGLTISS ARASKILRNE++G
Sbjct: 421 HGMPVEKYLEILGECRQKLFKVRERRPKPHLDDKVIVSWNGLTISSLARASKILRNEKEG 480

Query: 533 TRFYFPVVGSDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLI 592
           TRFYFPVVG D KEYL VAEKAALFI+TKLYDEQT RLQHSFRNGPS+APGFLDDYAFLI
Sbjct: 481 TRFYFPVVGCDPKEYLDVAEKAALFIKTKLYDEQTHRLQHSFRNGPSRAPGFLDDYAFLI 540

Query: 593 GGLLDLYEYG-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAE 652
           GGLLDLYEYG                       EGGGYYNT GED S++LRVKEDHDGAE
Sbjct: 541 GGLLDLYEYGGGLNWLVWAIELQATQDELFLDREGGGYYNTTGEDKSVILRVKEDHDGAE 600

Query: 653 PSGNSVSAINLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSV 712
           PSGNSVSAINLVRLSSLVSGS+S+YYRQNAEHLLAVFEKRLKD AVAVPLMCCAA M S 
Sbjct: 601 PSGNSVSAINLVRLSSLVSGSRSNYYRQNAEHLLAVFEKRLKDMAVAVPLMCCAAGMLST 660

Query: 713 PSRKHVVLVGHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMAT 772
           PSRK VVLVGHKNS QFET +AAAHASYDPN TVIHID TD+ E+QFWEENNR VA MA 
Sbjct: 661 PSRKQVVLVGHKNSAQFETFLAAAHASYDPNSTVIHIDPTDDTELQFWEENNRGVAVMAK 720

Query: 773 NNFAADKVVGMVCQNFTCKAPVSDPESLEAMLAQKPS 786
           NNFAADKVV +VCQNFTCKAP++DP SLEAMLA+KPS
Sbjct: 721 NNFAADKVVALVCQNFTCKAPITDPGSLEAMLAEKPS 757

BLAST of Cp4.1LG17g06880 vs. TAIR 10
Match: AT4G03200.1 (catalytics )

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 553/803 (68.87%), Postives = 640/803 (79.70%), Query Frame = 0

Query: 6   LRRFPRRRYPSEFSPNSTPIPFPRFPFLLSHSPLRFRAPFHACKVLAMAAQSSDG--GSH 65
           L   PRRR        S   PFP         P+  R P  + KVLAMA +SS     S 
Sbjct: 26  LSTLPRRRNIVRIHNPSFSSPFP---------PILSR-PISSGKVLAMAEESSSSSTSST 85

Query: 66  SHKYTNLLAVEHSPYLLQHAHNPVNWYPWGDEAFEEARKRNVPIFLSIGYSTCHWCHVME 125
           S K+TN LA EHSPYLLQHAHNPV+WYPWG+EAFEEARKR+VPIFLSIGYSTCHWCHVME
Sbjct: 86  SQKHTNRLAAEHSPYLLQHAHNPVDWYPWGEEAFEEARKRDVPIFLSIGYSTCHWCHVME 145

Query: 126 VESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLKPL 185
           VESFE+EEVAKLLN+ F+SIKVDREERPDVDKVYM++VQALY GGGWPLSVFLSPDLKPL
Sbjct: 146 VESFEDEEVAKLLNNSFVSIKVDREERPDVDKVYMSFVQALYGGGGWPLSVFLSPDLKPL 205

Query: 186 MGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNKLP 245
           MGGTYFPP+D YGRPGFKT+L+KVKDAW++KRD LVKSGTYAIE+LS+ALSAS  ++KL 
Sbjct: 206 MGGTYFPPNDNYGRPGFKTLLKKVKDAWNSKRDTLVKSGTYAIEELSKALSASTGADKLS 265

Query: 246 EELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAEES 305
           + + ++A+  CA+QLS+SYD  FGGFGSAPKFPRPVE+QLMLY+ K L+ESGK+ EA+E 
Sbjct: 266 DGISREAVSTCAKQLSRSYDSEFGGFGSAPKFPRPVEIQLMLYHYKKLKESGKTSEADEE 325

Query: 306 LNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSITK 365
            +MV+F LQ M+ GG+HDH+GGGFHRYSVDE WHVPHFEKMLYDQGQ+ANVYLD FSITK
Sbjct: 326 KSMVLFSLQGMANGGMHDHIGGGFHRYSVDECWHVPHFEKMLYDQGQLANVYLDGFSITK 385

Query: 366 DVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDDTL 425
           DV YS V+RD+LDYLRRDMI PEG I+SAEDADS E EGA RKKEGAFY+WTS E+D+ L
Sbjct: 386 DVMYSYVARDILDYLRRDMIAPEGGIFSAEDADSFEFEGAKRKKEGAFYIWTSDEIDEVL 445

Query: 426 GEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKYLEI 485
           GE+AD FKEHYY+K SGNCDLS  SDPH EF GKNVLIE    S +ASK  L VEKY EI
Sbjct: 446 GENADLFKEHYYVKKSGNCDLSSRSDPHNEFAGKNVLIERNETSAMASKFSLSVEKYQEI 505

Query: 486 LGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVVGSD 545
           LGECR KLF VR  RPKPHLDDK+IVSWNGL ISSFARASKIL+ E + T++YFPVV S 
Sbjct: 506 LGECRRKLFDVRLKRPKPHLDDKIIVSWNGLVISSFARASKILKAEPESTKYYFPVVNSQ 565

Query: 546 AKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEYG- 605
            ++Y++VAEKAALFIR  LYDEQ+RRLQHS+R GPSKAP FLDDYAFLI GLLDLYE G 
Sbjct: 566 PEDYIEVAEKAALFIRGNLYDEQSRRLQHSYRQGPSKAPAFLDDYAFLISGLLDLYENGG 625

Query: 606 ----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSAINL 665
                                 EGG Y+NT G+DPS+LLRVKEDHDGAEPSGNSVSAINL
Sbjct: 626 GIEWLKWAIKLQETQDELYLDREGGAYFNTEGQDPSVLLRVKEDHDGAEPSGNSVSAINL 685

Query: 666 VRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVLVGH 725
           VRL+S+V+G K++ Y   A  LLAVFE RL++ AVAVPLMCC+ADM SVPSRK VVLVG 
Sbjct: 686 VRLASIVAGEKAESYLNTAHRLLAVFELRLRELAVAVPLMCCSADMISVPSRKQVVLVGS 745

Query: 726 KNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKVVGM 784
           K+S +   M++AAH+ YDPN+TVIHID +   E++FWEE+N NVA MA  N  ++KVV +
Sbjct: 746 KSSPELTNMLSAAHSVYDPNKTVIHIDPSSSDEIEFWEEHNSNVAEMAKKNRNSEKVVAL 805

BLAST of Cp4.1LG17g06880 vs. TAIR 10
Match: AT4G03200.2 (catalytics )

HSP 1 Score: 987.3 bits (2551), Expect = 7.3e-288
Identity = 481/685 (70.22%), Postives = 561/685 (81.90%), Query Frame = 0

Query: 122 MEVESFENEEVAKLLNDWFISIKVDREERPDVDKVYMTYVQALYSGGGWPLSVFLSPDLK 181
           MEVESFE+EEVAKLLN+ F+SIKVDREERPDVDKVYM++VQALY GGGWPLSVFLSPDLK
Sbjct: 1   MEVESFEDEEVAKLLNNSFVSIKVDREERPDVDKVYMSFVQALYGGGGWPLSVFLSPDLK 60

Query: 182 PLMGGTYFPPDDKYGRPGFKTVLRKVKDAWDNKRDVLVKSGTYAIEQLSEALSASASSNK 241
           PLMGGTYFPP+D YGRPGFKT+L+KVKDAW++KRD LVKSGTYAIE+LS+ALSAS  ++K
Sbjct: 61  PLMGGTYFPPNDNYGRPGFKTLLKKVKDAWNSKRDTLVKSGTYAIEELSKALSASTGADK 120

Query: 242 LPEELPQKALRLCAEQLSQSYDPNFGGFGSAPKFPRPVEVQLMLYYTKILEESGKSVEAE 301
           L + + ++A+  CA+QLS+SYD  FGGFGSAPKFPRPVE+QLMLY+ K L+ESGK+ EA+
Sbjct: 121 LSDGISREAVSTCAKQLSRSYDSEFGGFGSAPKFPRPVEIQLMLYHYKKLKESGKTSEAD 180

Query: 302 ESLNMVIFGLQCMSRGGIHDHVGGGFHRYSVDERWHVPHFEKMLYDQGQIANVYLDAFSI 361
           E  +MV+F LQ M+ GG+HDH+GGGFHRYSVDE WHVPHFEKMLYDQGQ+ANVYLD FSI
Sbjct: 181 EEKSMVLFSLQGMANGGMHDHIGGGFHRYSVDECWHVPHFEKMLYDQGQLANVYLDGFSI 240

Query: 362 TKDVSYSCVSRDVLDYLRRDMIGPEGEIYSAEDADSAESEGATRKKEGAFYVWTSKEVDD 421
           TKDV YS V+RD+LDYLRRDMI PEG I+SAEDADS E EGA RKKEGAFY+WTS E+D+
Sbjct: 241 TKDVMYSYVARDILDYLRRDMIAPEGGIFSAEDADSFEFEGAKRKKEGAFYIWTSDEIDE 300

Query: 422 TLGEHADFFKEHYYIKPSGNCDLSRLSDPHGEFKGKNVLIEMKSVSEIASKHGLPVEKYL 481
            LGE+AD FKEHYY+K SGNCDLS  SDPH EF GKNVLIE    S +ASK  L VEKY 
Sbjct: 301 VLGENADLFKEHYYVKKSGNCDLSSRSDPHNEFAGKNVLIERNETSAMASKFSLSVEKYQ 360

Query: 482 EILGECRLKLFRVREHRPKPHLDDKVIVSWNGLTISSFARASKILRNEEDGTRFYFPVVG 541
           EILGECR KLF VR  RPKPHLDDK+IVSWNGL ISSFARASKIL+ E + T++YFPVV 
Sbjct: 361 EILGECRRKLFDVRLKRPKPHLDDKIIVSWNGLVISSFARASKILKAEPESTKYYFPVVN 420

Query: 542 SDAKEYLKVAEKAALFIRTKLYDEQTRRLQHSFRNGPSKAPGFLDDYAFLIGGLLDLYEY 601
           S  ++Y++VAEKAALFIR  LYDEQ+RRLQHS+R GPSKAP FLDDYAFLI GLLDLYE 
Sbjct: 421 SQPEDYIEVAEKAALFIRGNLYDEQSRRLQHSYRQGPSKAPAFLDDYAFLISGLLDLYEN 480

Query: 602 G-----------------------EGGGYYNTAGEDPSILLRVKEDHDGAEPSGNSVSAI 661
           G                       EGG Y+NT G+DPS+LLRVKEDHDGAEPSGNSVSAI
Sbjct: 481 GGGIEWLKWAIKLQETQDELYLDREGGAYFNTEGQDPSVLLRVKEDHDGAEPSGNSVSAI 540

Query: 662 NLVRLSSLVSGSKSDYYRQNAEHLLAVFEKRLKDTAVAVPLMCCAADMFSVPSRKHVVLV 721
           NLVRL+S+V+G K++ Y   A  LLAVFE RL++ AVAVPLMCC+ADM SVPSRK VVLV
Sbjct: 541 NLVRLASIVAGEKAESYLNTAHRLLAVFELRLRELAVAVPLMCCSADMISVPSRKQVVLV 600

Query: 722 GHKNSEQFETMVAAAHASYDPNRTVIHIDTTDEMEMQFWEENNRNVAAMATNNFAADKVV 781
           G K+S +   M++AAH+ YDPN+TVIHID +   E++FWEE+N NVA MA  N  ++KVV
Sbjct: 601 GSKSSPELTNMLSAAHSVYDPNKTVIHIDPSSSDEIEFWEEHNSNVAEMAKKNRNSEKVV 660

Query: 782 GMVCQNFTCKAPVSDPESLEAMLAQ 784
            +VCQ+FTC  PV D  SL  +L++
Sbjct: 661 ALVCQHFTCSPPVFDSSSLTRLLSK 685