Cp4.1LG17g06540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g06540
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	adenylate isopentenyltransferase 3, chloroplastic-like
Location	Cp4.1LG17: 6547060 .. 6548052 (-)
RNA-Seq Expression	Cp4.1LG17g06540
Synteny	Cp4.1LG17g06540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGTGTCTACCATGTTCATGAGCCAACAAATCACTCAACAACCATTGATAAATCTTCCCCCTCGAAGAATTAACACCAACCCTTTCTTCAACGTTAGGAAGCTCGAAAAGGAGAAGGTGGTGATTGTGATTGGCGCCACTGGAACGGGAAAATCTCGACTTTCGATCGATATTGCTACCCGATTTCCGTCGGAAGTCATCAATTCTGACAAAATGCAAGTCTACGACGGTCTGGATATAATAACAAATAAGGTCACTAAACAAGAACAACGCGGTGTGCCTCATCACTTACTTGGAATTATTGATCCTTTCTCTGACTTCTCGGCTACCAACTTCTCTGATATGGCTATGCTTTCTTTGGACACTATTCTCGGCCGTGACCGTCTCCCAATCATTGCTGGTGGCTCGAATTCTTACGTTGAAGCTTTAATTGACTCGAACTTTCAATCGAAATATGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCAGTACTTCATTCCTTTGTATCCAAACGAGTTGACAAGATGGTAGCAAATGGGATGGTCGACGAGGCCGAAGAATTCTTCGATCCGGCTGGTGATTATTCTCGTGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCGTATTTTCGATACAGATCGTTATTAAACGACGAAACTCAAGAGAAACTTTTGCAAGAAGCCATATCAAAAGTAAAGAAGTACACATGCAAGCTAGCATCGAGGCAAATGGGGAAGATCCAACGGCTGAGAAACATCAAGGGGTGGAAGATACACCGGTTGGATGCGACCGAGGCCTTTAAAAAACAAGGGAAAGAAGCCGAAGAAGAATGGAACAAGCTCGTTACAGTGCCTAGCACGACGATAATCGACCAGTTTCTTCACTCGAACGTCATCACCACAAAAGTCCCGACGAATTTAGCGACCCTCCTCGGCCTGCCGAGGAAGAGAAGTGCAGCCATTGCAGCTGCAACTCCTTGA

mRNA sequence

ATGAATGTGTCTACCATGTTCATGAGCCAACAAATCACTCAACAACCATTGATAAATCTTCCCCCTCGAAGAATTAACACCAACCCTTTCTTCAACGTTAGGAAGCTCGAAAAGGAGAAGGTGGTGATTGTGATTGGCGCCACTGGAACGGGAAAATCTCGACTTTCGATCGATATTGCTACCCGATTTCCGTCGGAAGTCATCAATTCTGACAAAATGCAAGTCTACGACGGTCTGGATATAATAACAAATAAGGTCACTAAACAAGAACAACGCGGTGTGCCTCATCACTTACTTGGAATTATTGATCCTTTCTCTGACTTCTCGGCTACCAACTTCTCTGATATGGCTATGCTTTCTTTGGACACTATTCTCGGCCGTGACCGTCTCCCAATCATTGCTGGTGGCTCGAATTCTTACGTTGAAGCTTTAATTGACTCGAACTTTCAATCGAAATATGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCAGTACTTCATTCCTTTGTATCCAAACGAGTTGACAAGATGGTAGCAAATGGGATGGTCGACGAGGCCGAAGAATTCTTCGATCCGGCTGGTGATTATTCTCGTGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCGTATTTTCGATACAGATCGTTATTAAACGACGAAACTCAAGAGAAACTTTTGCAAGAAGCCATATCAAAAGTAAAGAAGTACACATGCAAGCTAGCATCGAGGCAAATGGGGAAGATCCAACGGCTGAGAAACATCAAGGGGTGGAAGATACACCGGTTGGATGCGACCGAGGCCTTTAAAAAACAAGGGAAAGAAGCCGAAGAAGAATGGAACAAGCTCGTTACAGTGCCTAGCACGACGATAATCGACCAGTTTCTTCACTCGAACGTCATCACCACAAAAGTCCCGACGAATTTAGCGACCCTCCTCGGCCTGCCGAGGAAGAGAAGTGCAGCCATTGCAGCTGCAACTCCTTGA

Coding sequence (CDS)

ATGAATGTGTCTACCATGTTCATGAGCCAACAAATCACTCAACAACCATTGATAAATCTTCCCCCTCGAAGAATTAACACCAACCCTTTCTTCAACGTTAGGAAGCTCGAAAAGGAGAAGGTGGTGATTGTGATTGGCGCCACTGGAACGGGAAAATCTCGACTTTCGATCGATATTGCTACCCGATTTCCGTCGGAAGTCATCAATTCTGACAAAATGCAAGTCTACGACGGTCTGGATATAATAACAAATAAGGTCACTAAACAAGAACAACGCGGTGTGCCTCATCACTTACTTGGAATTATTGATCCTTTCTCTGACTTCTCGGCTACCAACTTCTCTGATATGGCTATGCTTTCTTTGGACACTATTCTCGGCCGTGACCGTCTCCCAATCATTGCTGGTGGCTCGAATTCTTACGTTGAAGCTTTAATTGACTCGAACTTTCAATCGAAATATGAATGTTGCTTCCTTTGGGTGGATGTGTCAATGCCAGTACTTCATTCCTTTGTATCCAAACGAGTTGACAAGATGGTAGCAAATGGGATGGTCGACGAGGCCGAAGAATTCTTCGATCCGGCTGGTGATTATTCTCGTGGGATCAAAAGGGCAATTGGGGTTCCCGAATTCGACTCGTATTTTCGATACAGATCGTTATTAAACGACGAAACTCAAGAGAAACTTTTGCAAGAAGCCATATCAAAAGTAAAGAAGTACACATGCAAGCTAGCATCGAGGCAAATGGGGAAGATCCAACGGCTGAGAAACATCAAGGGGTGGAAGATACACCGGTTGGATGCGACCGAGGCCTTTAAAAAACAAGGGAAAGAAGCCGAAGAAGAATGGAACAAGCTCGTTACAGTGCCTAGCACGACGATAATCGACCAGTTTCTTCACTCGAACGTCATCACCACAAAAGTCCCGACGAATTTAGCGACCCTCCTCGGCCTGCCGAGGAAGAGAAGTGCAGCCATTGCAGCTGCAACTCCTTGA

Protein sequence

MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHSNVITTKVPTNLATLLGLPRKRSAAIAAATP

Homology

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BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot
Match: Q93WC9 (Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT3 PE=1 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.2e-88
Identity = 162/304 (53.29%), Postives = 216/304 (71.05%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     Q +   P ++ PP R   N         K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRF +E+INSDK+QV+ GLDI+TNK+T +E  GVPHHLLG++ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDTILGRDRLPIIAGGSNSYVEALID---SNFQSKYECCFLWVDVSMPVLHSFVSKRVDK 180
           ++++L R +LPII GGSNSYVEAL+D   + F+S+Y+CCFLWVDV++PVLH FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVANGMVDEAEEFFD-PAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKV 240
           MV +GMV+E  EFFD    DYSRGIK+AIG PEFD +FR    LN E +E+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK--EAEEEWNKLVTVPSTTII 299
           K+ T +LA RQ  KI+RLR +K W I R+DAT  F K+    +A   W +LV  PST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot
Match: Q94ID2 (Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT5 PE=1 SV=2)

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-83
Identity = 161/294 (54.76%), Postives = 213/294 (72.45%), Query Frame = 0


Query: 10  QQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVIN 69
           +Q+ Q   +N     ++  PFF      K+KVV V+GATGTGKSRL+ID+ATRFP+E++N
Sbjct: 9   RQVIQPLSLNFQGNMVDV-PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVN 68

Query: 70  SDKMQVYDGLDIITNKVTKQEQRGVPHHLLGII-DPFSDFSATNFSDMAMLSLDTILGRD 129
           SDK+QVY GLDI+TNKVT +E  GVPHHLLG + D + DF+A +F   A+ ++++I+ RD
Sbjct: 69  SDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRD 128

Query: 130 RLPIIAGGSNSYVEALIDS--NFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDE 189
           R+PIIAGGSNSY+EAL++   +F+ +Y CCFLWVDVS PVLHSFVS+RVDKMV  G+VDE
Sbjct: 129 RVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDE 188

Query: 190 AEEFFDP-AGDYSRGIKRAIGVPEFDSYFRYRSL-LNDETQEKLLQEAISKVKKYTCKLA 249
               FDP + DYS GI+RAIGVPE D + R        ET E+LL+ AI K+K+ TC LA
Sbjct: 189 VRRIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLA 248

Query: 250 SRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFL 299
            RQ+ KIQRL     W +HR+DATE F ++G+EA+E W+  V  PS   +++FL
Sbjct: 249 CRQLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot
Match: Q94ID1 (Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IPT7 PE=2 SV=2)

HSP 1 Score: 285.4 bits (729), Expect = 8.1e-76
Identity = 146/285 (51.23%), Postives = 204/285 (71.58%), Query Frame = 0


Query: 24  RINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIIT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKQEQRGVPHHLLGIID-PFSDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNSYVE 143
           NKVT +E RGVPHHLLG+ D    + +AT +S +A  ++  +   ++LPI+AGGSNSY+E
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSN---FQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSR 203
           AL++ +     + Y+CCF+WVDVS+PVL+SFVSKRVD+M+  G+++E  E F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKG 263
           GI+RAIGVPE   Y R  SL++  T+ K+L  A+  +KK T  LA RQ+ KIQRL   K 
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KK 262

Query: 264 WK--IHRLDATEAF-KKQGKEAEEEWNKLVTVPSTTIIDQFLHSN 302
           WK  +HR+DATE F K+  +E +E W  LV  PS  I+D+F ++N
Sbjct: 263 WKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 245.4 bits (625), Expect = 9.3e-64
Identity = 140/336 (41.67%), Postives = 203/336 (60.42%), Query Frame = 0


Query: 4   STMFMSQQITQQPLINLPPRRINTNPFFNVRKLE-------KEKVVIVIGATGTGKSRLS 63
           S  F S   +   L++   RR    P  +  ++E       K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDP-FSDFSATNFSD 123
           +D+ATRFPSE+INSDK+QVY+GL+I TN++T Q++RGVPHHLLG+I+P   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDTILGRDRLPIIAGGSNSYVEALIDSNFQSK----------------YECCFLW 183
            A   +  I  R ++PIIAGGSNS+V AL+   F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDP--AGDYSR-GIKRAIGVPEFDSYFR- 243
           VDVS  VL+ ++ +RVD+M+ +GM +E   F+DP  +G  +R GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YRSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAF 302
                + +  D  ++    +A+  +K+ T  LA RQ+ KI+ L++  GW+I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKD-AGWEIERVDATASF 322

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.1e-63
Identity = 136/317 (42.90%), Postives = 189/317 (59.62%), Query Frame = 0


Query: 21  PPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLD 80
           P     TN     ++  KEK+++++GATGTGKSRLSID+A  FP EVINSDKMQVY GLD
Sbjct: 12  PTTTTTTNVSLRRQRHRKEKLLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLD 71

Query: 81  IITNKVTKQEQRGVPHHLLGIIDPF-SDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNS 140
           I TNK++  ++ GVPHHLLG +DP   + +  +F  +A  ++  I GR +LP++ GGSNS
Sbjct: 72  ITTNKISVPDRGGVPHHLLGEVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNS 131

Query: 141 YVEALIDSNFQS------------------KYECCFLWVDVSMPVLHSFVSKRVDKMVAN 200
           ++ AL+   F S                  +Y+CCFLWVDVS+ VL  +++KRVD M+  
Sbjct: 132 FIHALLVDRFDSSGPGVFEEGSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLEL 191

Query: 201 GMVDEAEEFFDPAGD-------YSRGIKRAIGVPEFDSYF-RYR-------SLLNDETQE 260
           GM DE  EF+ P  +          G+++AIGVPEFD YF ++R           D  + 
Sbjct: 192 GMFDELAEFYSPEDEDHDEDSATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRR 251

Query: 261 KLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFK-----KQGKEAEEE 299
              +EA+  +K+ TC LA RQ+GKI RL+   GW + RLDATE+F+       G++  E 
Sbjct: 252 GAFEEAVRAIKENTCHLAKRQIGKILRLKG-AGWDLRRLDATESFRAAMTSDSGEKCTEI 311

[Close]

BLAST of Cp4.1LG17g06540 vs. NCBI nr
Match: XP_023514910.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648 bits (1672), Expect = 1.97e-234
Identity = 330/330 (100.00%), Postives = 330/330 (100.00%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITTKVPTNLATLLGLPRKRSAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. NCBI nr
Match: KAG7025533.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639 bits (1648), Expect = 8.96e-231
Identity = 326/330 (98.79%), Postives = 327/330 (99.09%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDTI GRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LDTIFGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITTKVPTNLATLL L RKRSAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRKRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. NCBI nr
Match: XP_023004596.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 635 bits (1638), Expect = 2.99e-229
Identity = 320/330 (96.97%), Postives = 329/330 (99.70%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDTILGRDRLPIIAGGSNSY+EALIDSNFQSKYECCFLWVDVSMP+LHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMV+EA+EFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTII+QFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITT VPTNLA+LLGLPRKRSAAIAAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. NCBI nr
Match: XP_022960175.1 (adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 634 bits (1636), Expect = 6.04e-229
Identity = 324/330 (98.18%), Postives = 326/330 (98.79%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           L TILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITTKVPTNLATLL L R RSAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. NCBI nr
Match: KAG6593132.1 (Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564 bits (1453), Expect = 9.50e-202
Identity = 284/287 (98.95%), Postives = 285/287 (99.30%), Query Frame = 0


Query: 6   MFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPS 65
           MFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPS
Sbjct: 1   MFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPS 60

Query: 66  EVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLSLDTIL 125
           EVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLSLDTI 
Sbjct: 61  EVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLSLDTIF 120

Query: 126 GRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVD 185
           GRDRLPIIAGGSNSYVEALIDSNFQSKYE CFLWVDVSMPVLHSFVSKRVDKMVANGMVD
Sbjct: 121 GRDRLPIIAGGSNSYVEALIDSNFQSKYEYCFLWVDVSMPVLHSFVSKRVDKMVANGMVD 180

Query: 186 EAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYTCKLAS 245
           EAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYTCKLAS
Sbjct: 181 EAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYTCKLAS 240

Query: 246 RQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTT 292
           RQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTT
Sbjct: 241 RQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTT 287

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL
Match: A0A6J1KSJ2 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111497849 PE=4 SV=1)

HSP 1 Score: 635 bits (1638), Expect = 1.45e-229
Identity = 320/330 (96.97%), Postives = 329/330 (99.70%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LDTILGRDRLPIIAGGSNSY+EALIDSNFQSKYECCFLWVDVSMP+LHSFVSKRVDKMVA
Sbjct: 121 LDTILGRDRLPIIAGGSNSYIEALIDSNFQSKYECCFLWVDVSMPILHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMV+EA+EFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVNEAKEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTII+QFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIEQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITT VPTNLA+LLGLPRKRSAAIAAATP
Sbjct: 301 NVITTNVPTNLASLLGLPRKRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL
Match: A0A6J1H8C9 (adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111460993 PE=4 SV=1)

HSP 1 Score: 634 bits (1636), Expect = 2.93e-229
Identity = 324/330 (98.18%), Postives = 326/330 (98.79%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA
Sbjct: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVYDGLDI+TNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYDGLDIVTNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           L TILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA
Sbjct: 121 LVTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLL+DETQEKLLQEAISKVKKYT
Sbjct: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLDDETQEKLLQEAISKVKKYT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS
Sbjct: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVITTKVPTNLATLL L R RSAAIAAATP
Sbjct: 301 NVITTKVPTNLATLLDLSRMRSAAIAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL
Match: A0A5D3DBM7 (Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00080 PE=4 SV=1)

HSP 1 Score: 553 bits (1424), Expect = 5.88e-197
Identity = 276/330 (83.64%), Postives = 304/330 (92.12%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPP R+NTN FFN+RKL+KEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFFNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVY GLDI+TNK+TKQEQ GVPHHLLGII+PF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I   DRLPIIAGGSNSY+EALIDSNF+ KYECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFR RSLL++E QEKLL+E+I+KVK++T
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLA +Q+GKIQRLRNIKGW IHRLDATEAF KQGKEAEE WNKLVT+PSTTIIDQFLH 
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           N + TK PTNLA+L+G+PRKRSAA+AAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL
Match: A0A0A0K919 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.96e-195
Identity = 273/330 (82.73%), Postives = 303/330 (91.82%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPP R+ TNPF  +RKL+KEKVVIVIG TGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSKITTQQPLINLPPGRLTTNPFSTLRKLQKEKVVIVIGPTGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRFPSEVINSDKMQVY GLDI+TNK+TKQEQ GVPHHLLGII+PF DFSA+NFSDMAMLS
Sbjct: 61  TRFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I   DRLPIIAGGSNSY+EALIDSNF+ +YECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLRYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFRYRSLL++E QEKLL+E+I+KVK++T
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRYRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLA RQ+GKIQRLRN+KGW IHRLDATEAF KQGKEA+E WNK VT+PSTTIIDQFLH 
Sbjct: 241 CKLALRQLGKIQRLRNVKGWNIHRLDATEAFTKQGKEADEAWNKHVTIPSTTIIDQFLHQ 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           NVI TK PTNLA+L+G+PRK+SAA+AAATP
Sbjct: 301 NVIATKFPTNLASLIGVPRKKSAAMAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL
Match: A0A1S3BRC2 (adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492689 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 3.96e-195
Identity = 274/330 (83.03%), Postives = 303/330 (91.82%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           MNVS+MFMS+  TQQPLINLPP R+NTN F N+RKL+KEKVV VIGATGTGKSRLSIDIA
Sbjct: 1   MNVSSMFMSEITTQQPLINLPPGRLNTNHFSNLRKLQKEKVVFVIGATGTGKSRLSIDIA 60

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           T+FPSEVINSDKMQVY GLDI+TNK+TKQEQ GVPHHLLGII+PF DFSA+NFSDMAMLS
Sbjct: 61  TQFPSEVINSDKMQVYHGLDIVTNKITKQEQCGVPHHLLGIINPFLDFSASNFSDMAMLS 120

Query: 121 LDTILGRDRLPIIAGGSNSYVEALIDSNFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVA 180
           LD I   DRLPIIAGGSNSY+EALIDSNF+ KYECCFLWVDVSM VL SFVS+RVDKMVA
Sbjct: 121 LDAISSHDRLPIIAGGSNSYIEALIDSNFRLKYECCFLWVDVSMQVLQSFVSERVDKMVA 180

Query: 181 NGMVDEAEEFFDPAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYT 240
           NGMVDEAE+FFDPA DYSRGIKRAIGVPEFDSYFR RSLL++E QEKLL+E+I+KVK++T
Sbjct: 181 NGMVDEAEKFFDPARDYSRGIKRAIGVPEFDSYFRCRSLLDEEAQEKLLEESIAKVKEHT 240

Query: 241 CKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFLHS 300
           CKLA +Q+GKIQRLRNIKGW IHRLDATEAF KQGKEAEE WNKLVT+PSTTIIDQFLH 
Sbjct: 241 CKLALKQLGKIQRLRNIKGWNIHRLDATEAFTKQGKEAEEAWNKLVTIPSTTIIDQFLHP 300

Query: 301 NVITTKVPTNLATLLGLPRKRSAAIAAATP 330
           N + TK PTNLA+L+G+PRKRSAA+AAATP
Sbjct: 301 NAVATKFPTNLASLIGVPRKRSAAMAAATP 330

[Close]

BLAST of Cp4.1LG17g06540 vs. TAIR 10
Match: AT3G63110.1 (isopentenyltransferase 3 )

HSP 1 Score: 326.6 bits (836), Expect = 2.3e-89
Identity = 162/304 (53.29%), Postives = 216/304 (71.05%), Query Frame = 0


Query: 1   MNVSTMFMSQQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIA 60
           M +S     Q +   P ++ PP R   N         K+KVV+++GATGTGKSRLS+DIA
Sbjct: 3   MKISMAMCKQPLPPSPTLDFPPARFGPNMLTLNPYGPKDKVVVIMGATGTGKSRLSVDIA 62

Query: 61  TRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDPFSDFSATNFSDMAMLS 120
           TRF +E+INSDK+QV+ GLDI+TNK+T +E  GVPHHLLG++ P +D +A N+  MA LS
Sbjct: 63  TRFRAEIINSDKIQVHQGLDIVTNKITSEESCGVPHHLLGVLPPEADLTAANYCHMANLS 122

Query: 121 LDTILGRDRLPIIAGGSNSYVEALID---SNFQSKYECCFLWVDVSMPVLHSFVSKRVDK 180
           ++++L R +LPII GGSNSYVEAL+D   + F+S+Y+CCFLWVDV++PVLH FVS+RVDK
Sbjct: 123 IESVLNRGKLPIIVGGSNSYVEALVDDKENKFRSRYDCCFLWVDVALPVLHGFVSERVDK 182

Query: 181 MVANGMVDEAEEFFD-PAGDYSRGIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKV 240
           MV +GMV+E  EFFD    DYSRGIK+AIG PEFD +FR    LN E +E+LL + + ++
Sbjct: 183 MVESGMVEEVREFFDFSNSDYSRGIKKAIGFPEFDRFFRNEQFLNVEDREELLSKVLEEI 242

Query: 241 KKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAFKKQGK--EAEEEWNKLVTVPSTTII 299
           K+ T +LA RQ  KI+RLR +K W I R+DAT  F K+    +A   W +LV  PST  +
Sbjct: 243 KRNTFELACRQREKIERLRKVKKWSIQRVDATPVFTKRRSKMDANVAWERLVAGPSTDTV 302

[Close]

BLAST of Cp4.1LG17g06540 vs. TAIR 10
Match: AT5G19040.1 (isopentenyltransferase 5 )

HSP 1 Score: 311.6 bits (797), Expect = 7.5e-85
Identity = 161/294 (54.76%), Postives = 213/294 (72.45%), Query Frame = 0


Query: 10  QQITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVIN 69
           +Q+ Q   +N     ++  PFF      K+KVV V+GATGTGKSRL+ID+ATRFP+E++N
Sbjct: 9   RQVIQPLSLNFQGNMVDV-PFFR----RKDKVVFVMGATGTGKSRLAIDLATRFPAEIVN 68

Query: 70  SDKMQVYDGLDIITNKVTKQEQRGVPHHLLGII-DPFSDFSATNFSDMAMLSLDTILGRD 129
           SDK+QVY GLDI+TNKVT +E  GVPHHLLG + D + DF+A +F   A+ ++++I+ RD
Sbjct: 69  SDKIQVYKGLDIVTNKVTPEESLGVPHHLLGTVHDTYEDFTAEDFQREAIRAVESIVQRD 128

Query: 130 RLPIIAGGSNSYVEALIDS--NFQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDE 189
           R+PIIAGGSNSY+EAL++   +F+ +Y CCFLWVDVS PVLHSFVS+RVDKMV  G+VDE
Sbjct: 129 RVPIIAGGSNSYIEALVNDCVDFRLRYNCCFLWVDVSRPVLHSFVSERVDKMVDMGLVDE 188

Query: 190 AEEFFDP-AGDYSRGIKRAIGVPEFDSYFRYRSL-LNDETQEKLLQEAISKVKKYTCKLA 249
               FDP + DYS GI+RAIGVPE D + R        ET E+LL+ AI K+K+ TC LA
Sbjct: 189 VRRIFDPSSSDYSAGIRRAIGVPELDEFLRSEMRNYPAETTERLLETAIEKIKENTCLLA 248

Query: 250 SRQMGKIQRLRNIKGWKIHRLDATEAFKKQGKEAEEEWNKLVTVPSTTIIDQFL 299
            RQ+ KIQRL     W +HR+DATE F ++G+EA+E W+  V  PS   +++FL
Sbjct: 249 CRQLQKIQRLYKQWKWNMHRVDATEVFLRRGEEADEAWDNSVAHPSALAVEKFL 297

[Close]

BLAST of Cp4.1LG17g06540 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 285.4 bits (729), Expect = 5.8e-77
Identity = 146/285 (51.23%), Postives = 204/285 (71.58%), Query Frame = 0


Query: 24  RINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSIDIATRFPSEVINSDKMQVYDGLDIIT 83
           ++N N F +     KEKV+ V+GATG+GKSRL+ID+ATRF  E+INSDK+Q+Y GLD++T
Sbjct: 23  KVNVNSFLH----PKEKVIFVMGATGSGKSRLAIDLATRFQGEIINSDKIQLYKGLDVLT 82

Query: 84  NKVTKQEQRGVPHHLLGIID-PFSDFSATNFSDMAMLSLDTILGRDRLPIIAGGSNSYVE 143
           NKVT +E RGVPHHLLG+ D    + +AT +S +A  ++  +   ++LPI+AGGSNSY+E
Sbjct: 83  NKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKLSANNKLPIVAGGSNSYIE 142

Query: 144 ALIDSN---FQSKYECCFLWVDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDPAGDYSR 203
           AL++ +     + Y+CCF+WVDVS+PVL+SFVSKRVD+M+  G+++E  E F+P  +YS 
Sbjct: 143 ALVNHSSGFLLNNYDCCFIWVDVSLPVLNSFVSKRVDRMMEAGLLEEVREVFNPKANYSV 202

Query: 204 GIKRAIGVPEFDSYFRYRSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKG 263
           GI+RAIGVPE   Y R  SL++  T+ K+L  A+  +KK T  LA RQ+ KIQRL   K 
Sbjct: 203 GIRRAIGVPELHEYLRNESLVDRATKSKMLDVAVKNIKKNTEILACRQLKKIQRLH--KK 262

Query: 264 WK--IHRLDATEAF-KKQGKEAEEEWNKLVTVPSTTIIDQFLHSN 302
           WK  +HR+DATE F K+  +E +E W  LV  PS  I+D+F ++N
Sbjct: 263 WKMSMHRVDATEVFLKRNVEEQDEAWENLVARPSERIVDKFYNNN 301

[Close]

BLAST of Cp4.1LG17g06540 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 245.4 bits (625), Expect = 6.6e-65
Identity = 140/336 (41.67%), Postives = 203/336 (60.42%), Query Frame = 0


Query: 4   STMFMSQQITQQPLINLPPRRINTNPFFNVRKLE-------KEKVVIVIGATGTGKSRLS 63
           S  F S   +   L++   RR    P  +  ++E       K+KVV+++GATG GKSRLS
Sbjct: 23  SPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSRLS 82

Query: 64  IDIATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDP-FSDFSATNFSD 123
           +D+ATRFPSE+INSDK+QVY+GL+I TN++T Q++RGVPHHLLG+I+P   + +A  F  
Sbjct: 83  VDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEFRS 142

Query: 124 MAMLSLDTILGRDRLPIIAGGSNSYVEALIDSNFQSK----------------YECCFLW 183
            A   +  I  R ++PIIAGGSNS+V AL+   F  K                YECCF+W
Sbjct: 143 AASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCFIW 202

Query: 184 VDVSMPVLHSFVSKRVDKMVANGMVDEAEEFFDP--AGDYSR-GIKRAIGVPEFDSYFR- 243
           VDVS  VL+ ++ +RVD+M+ +GM +E   F+DP  +G  +R GI++AIGVPEFD YF+ 
Sbjct: 203 VDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYFKE 262

Query: 244 ----YRSLLNDETQEKLLQEAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDATEAF 302
                + +  D  ++    +A+  +K+ T  LA RQ+ KI+ L++  GW+I R+DAT +F
Sbjct: 263 YPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKD-AGWEIERVDATASF 322

[Close]

BLAST of Cp4.1LG17g06540 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 229.6 bits (584), Expect = 3.7e-60
Identity = 134/330 (40.61%), Postives = 198/330 (60.00%), Query Frame = 0


Query: 2   NVSTMFMSQQ---ITQQPLINLPPRRINTNPFFNVRKLEKEKVVIVIGATGTGKSRLSID 61
           N+++ F+S     IT   L   PPR +       + +  K+KVV+++GATG+GKS LSID
Sbjct: 3   NLTSTFVSPSMIPITSPRLRLPPPRSVVPMTTVCMEQSYKQKVVVIMGATGSGKSCLSID 62

Query: 62  IATRFPSEVINSDKMQVYDGLDIITNKVTKQEQRGVPHHLLGIIDP-FSDFSATNFSDMA 121
           +ATRF  E++NSDK+Q YDGL + TN+++  E+ GVPHHLLG + P  S+ + + F  +A
Sbjct: 63  LATRFSGEIVNSDKIQFYDGLKVTTNQMSILERCGVPHHLLGELPPDDSELTTSEFRSLA 122

Query: 122 MLSLDTILGRDRLPIIAGGSNSYVEALIDSNFQSK---------------YECCFLWVDV 181
             S+  I  R  LPIIAGGSNS++ AL+   F  K               YECCFLWVDV
Sbjct: 123 SRSISEITARGNLPIIAGGSNSFIHALLVDRFDPKTYPFSSETSISSGLRYECCFLWVDV 182

Query: 182 SMPVLHSFVSKRVDKMVANGMVDEAEEFFDP----AGDYSRGIKRAIGVPEFDSYFRYRS 241
           S+ VL  ++SKRVD+M+ +GM +E   F+DP    +   + GI + IG+PEFD YF   S
Sbjct: 183 SVSVLFEYLSKRVDQMMESGMFEELAGFYDPRYSGSAIRAHGIHKTIGIPEFDRYF---S 242

Query: 242 LLNDETQEKLLQ----------EAISKVKKYTCKLASRQMGKIQRLRNIKGWKIHRLDAT 299
           L   E ++K+ +          EA+ ++K+ T +LA +Q+ +I +L++  GW I RLDAT
Sbjct: 243 LYPPERKQKMSEWDQARKGAYDEAVQEIKENTWRLAKKQIERIMKLKS-SGWDIQRLDAT 302

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g06540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WC9	3.2e-88	53.29	Adenylate isopentenyltransferase 3, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID2	1.1e-83	54.76	Adenylate isopentenyltransferase 5, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q94ID1	8.1e-76	51.23	Adenylate isopentenyltransferase 7, mitochondrial OS=Arabidopsis thaliana OX=370...	[more]
Q94ID3	9.3e-64	41.67	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q5GHF7	2.1e-63	42.90	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]

BLAST of Cp4.1LG17g06540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514910.1	1.97e-234	100.00	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita pepo subsp. pe...	[more]
KAG7025533.1	8.96e-231	98.79	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_023004596.1	2.99e-229	96.97	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita maxima]	[more]
XP_022960175.1	6.04e-229	98.18	adenylate isopentenyltransferase 3, chloroplastic-like [Cucurbita moschata]	[more]
KAG6593132.1	9.50e-202	98.95	Adenylate isopentenyltransferase 3, chloroplastic, partial [Cucurbita argyrosper...	[more]

BLAST of Cp4.1LG17g06540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KSJ2	1.45e-229	96.97	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H8C9	2.93e-229	98.18	adenylate isopentenyltransferase 3, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A5D3DBM7	5.88e-197	83.64	Adenylate isopentenyltransferase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A0A0K919	3.96e-195	82.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G392940 PE=4 SV=1	[more]
A0A1S3BRC2	3.96e-195	83.03	adenylate isopentenyltransferase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC...	[more]

BLAST of Cp4.1LG17g06540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63110.1	2.3e-89	53.29	isopentenyltransferase 3	[more]
AT5G19040.1	7.5e-85	54.76	isopentenyltransferase 5	[more]
AT3G23630.1	5.8e-77	51.23	isopentenyltransferase 7	[more]
AT1G68460.1	6.6e-65	41.67	isopentenyltransferase 1	[more]
AT3G19160.1	3.7e-60	40.61	ATP/ADP isopentenyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 164..262 e-value: 1.2E-108 score: 364.3
None	No IPR available	PFAM	PF01715	IPPT	coord: 74..147 e-value: 1.4E-21 score: 77.3 coord: 151..249 e-value: 3.4E-12 score: 46.6
None	No IPR available	PANTHER	PTHR11088:SF74	ADENYLATE ISOPENTENYLTRANSFERASE 5, CHLOROPLASTIC	coord: 34..302
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 38..298 e-value: 1.2E-108 score: 364.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 37..191
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 34..302

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g06540.1	Cp4.1LG17g06540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009691	cytokinin biosynthetic process
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005634	nucleus
cellular_component	GO:0009536	plastid
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity