Cp4.1LG17g06100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g06100
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	hyoscyamine 6-dioxygenase-like
Location	Cp4.1LG17: 6921494 .. 6923627 (+)
RNA-Seq Expression	Cp4.1LG17g06100
Synteny	Cp4.1LG17g06100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACACCATTCCCGTCCTCGATCTCTCTACCCGTGATCGACCTCTTCTCGTTCGTAAAATCCTCGATGCTTCTCAAGAATTCGGATTTTTTCAGGTTTCAATTCTCTCTTTCGTGTTCTTCGATACTTCTGATTCTTTTTCGATTATGATTGATTGTTTCGTTTCTTTTTGTTGTTGTTACGTATCGAACTATTTTGTTTTCGTCTCGTGAAATTCGAGTTGGGTTTTTAGAATTTTGATAATGTAAATGTTCAACGACCCACCTATATTGATCTAAATATAATTTACTTAATTAAGACATATATGGTAACGGTTCAAGCCCACCATTAGTAGATATTGTCTGTTTTTGCCTGTCACGTATCATCGTCAGCTTCACGGTATTAAAACGTGTCTGCTAGAGAGAAGTTTTCACACCCTTATAAGAAATGTTTCGTTCCAGACTCCAACCGACGTGGGATCTCATATATATATATATATATATATATTACCTTTTTGCTTGGGAATATGTCTCTTTTTCCCCTTTAGTTTCTAGTGTTTGTATGAATATGCCTTGGAAACTTACCTTTTTTACCATAATTTTGTAAAAAAAAATTAAAATTAAAAAAAACCCAATATATATTTTAACTATTTTCATCGAAGATTTTAATATTTTAGGTAGACGACGGATCGTTTTCAAATTAGGTCAGAATATAACTGAAGCTTAACCGTTAATTTTTTTCCGATGAACTTTGTCACATCCAACCATTATTTTCGGTGAAAGATTGCACATGAATATAATTAATTCAACTTAAAAGCTAAATGTCAACTCTATTTAAGATTCAAAAGCGGGCAATAACATTGAATAATACTATAATTCATAAATTTTCAAATGTTTCGGTCTCGAGAATATATGACCTAATCAGTTAATTCTTGTTTTAGGTGATAAACCACGGAGTGTCGAAAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCACGCGATGTCGGGAGCTGAGAAGGCGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGGTAAAATTACATATATACCCTTTCCATTTTTACTAATTATCAATAATCTTTTTTAATTTTTAATTTTTTTATTTATAAATATAAAATTATTTTAAAATATCACATAATTATTTATAAGCTCATAATTTTTTAAAAAAATTGAAATTAATTATTGAAAAATAATTCAAGATAATTAATCGTCATTTTAATAAATATTTAATTATGATATTGTTGACGTATTGCAGAGAAGTGGTCGGAGCGTATTGTGTTGAGATGAGAGCGTTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTAGTTAACCATTACCCACCGTGTCCAAACCCGAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTTCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAACATTGGCTTCGTTCTACAGGTACGTTTAAAGTGAGTTTGACCCGTGTTTCTTTGAAGATTTACTTATATGATGGTTGTTACGTAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACACCGAGCCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTGGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGCGATACCAAGGCGGTGATGGAGTGTATTGGCTCCGATCGCTCTTAA

mRNA sequence

ATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACACCATTCCCGTCCTCGATCTCTCTACCCGTGATCGACCTCTTCTCGTTCGTAAAATCCTCGATGCTTCTCAAGAATTCGGATTTTTTCAGGTGATAAACCACGGAGTGTCGAAAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCACGCGATGTCGGGAGCTGAGAAGGCGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGAGAAGTGGTCGGAGCGTATTGTGTTGAGATGAGAGCGTTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTAGTTAACCATTACCCACCGTGTCCAAACCCGAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTTCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAACATTGGCTTCGTTCTACAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACACCGAGCCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTGGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGCGATACCAAGGCGGTGATGGAGTGTATTGGCTCCGATCGCTCTTAA

Coding sequence (CDS)

ATGGGAGAGGTCAAGTTCGTGTCCAATTGGGAGGGTGTTCAATCTGTACCACCAGATTATGTCTATCCCCCGGAGAAGAGGCCCGGCAACGTCGTCGTTCCGATGGCCAACACCATTCCCGTCCTCGATCTCTCTACCCGTGATCGACCTCTTCTCGTTCGTAAAATCCTCGATGCTTCTCAAGAATTCGGATTTTTTCAGGTGATAAACCACGGAGTGTCGAAAAGGGTATTAGAGGAAACGACGAGGATATTCAAAAAATTTCACGCGATGTCGGGAGCTGAGAAGGCGAAGGAATGCTCGAGAGACCCGAACAAAAGTTGTAGGGTTTACACGAGTAGTGAGAATTATAGGAAGGAACAAACTCACTGTTGGAGAGATGCTTTGATCTTCAACTGCCATCCTTTGGACAAATATGTTCATTTTTGGCCTCAAAATCCCCCAAAATACAGAGAAGTGGTCGGAGCGTATTGTGTTGAGATGAGAGCGTTGGTTTTGGAGATATTAGAACTCGTAAGCGAAGGATTGGGACTCGGGAGAGGATACTTAGGGGGTGAAATGAGTGAAAATCCGTTGATGTTAGTTAACCATTACCCACCGTGTCCAAACCCGAGCTTGACCTTGGGATTGAGCCAACATTGTGATCCAAGCCTCATCACCATCTTGTTTCAAGAGGTCAATGGCCTTCAAGTTCTCAAGGATGGTCAATGGATTGGCGTTCATCCTATCGATAACGCGTTTGTTGTTAACATTGGCTTCGTTCTACAGGTGATTAGTAATGGGAAGCTAAAAGCAGCAGAACACCGAGCCGTGACGAACGCGAAAATATCTCGACAAAGCCTAACGTATTTGGTGTATCCAAAGGATGAAGTGACTATGGAACCAGCAAAATGTTTGATAAATGAAGCCAACCCTCCACGATATCGAGCCCTCAAGTTCAAAGACTTTCAACGAAACTACCTACCAAGGGCTAGCGATACCAAGGCGGTGATGGAGTGTATTGGCTCCGATCGCTCTTAA

Protein sequence

MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS

Homology

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BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot
Match: P24397 (Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-68
Identity = 140/332 (42.17%), Postives = 197/332 (59.34%), Query Frame = 0


Query: 6   FVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFGF 65
           FVSNW   +SV   ++ P +KR     VP+ N +P++DL  +   LLV++I  A Q+FG 
Sbjct: 4   FVSNW-STKSVSESFIAPLQKR-AEKDVPVGNDVPIIDLQ-QHHHLLVQQITKACQDFGL 63

Query: 66  FQVINHGVSKRVLEETTRIFKKFHAMSGAEKAK--------ECSRDPNKSCRVYTSSENY 125
           FQVINHG  + ++ ET  + K+F A+   EK K        +      +  ++Y   E  
Sbjct: 64  FQVINHGFPEELMLETMEVCKEFFALPAEEKEKFKPKGEAAKFELPLEQKAKLYVEGEQL 123

Query: 126 RKEQTHCWRDALIFNCHPLDK-YVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGL 185
             E+   W+D L   CHPLD+  V+ WP+ P KYREVV  Y VE+R L + +L+ + EGL
Sbjct: 124 SNEEFLYWKDTLAHGCHPLDQDLVNSWPEKPAKYREVVAKYSVEVRKLTMRMLDYICEGL 183

Query: 186 GLGRGYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQ--VLKD 245
           GL  GY   E+S+  +ML N+YPPCP+PS TLG   H D +LIT+L Q++ GLQ  ++KD
Sbjct: 184 GLKLGYFDNELSQIQMMLTNYYPPCPDPSSTLGSGGHYDGNLITLLQQDLPGLQQLIVKD 243

Query: 246 GQWIGVHPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTME 305
             WI V PI  AFVVN+G  L+VI+N K + + HR VT+    R S+  L+ P    T+E
Sbjct: 244 ATWIAVQPIPTAFVVNLGLTLKVITNEKFEGSIHRVVTDPTRDRVSIATLIGPDYSCTIE 303

Query: 306 PAKCLINEANPPRYRALKFKDFQRNYLPRASD 327
           PAK L+N+ NPP Y+   + +F   YL   SD
Sbjct: 304 PAKELLNQDNPPLYKPYSYSEFADIYLSDKSD 332

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BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.9e-68
Identity = 139/334 (41.62%), Postives = 200/334 (59.88%), Query Frame = 0


Query: 5   KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFG 64
           K +S      ++P +YV P   RP    V      P++DLS+ DR  L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHC 124
           FFQVINHGV+K++++E   + ++F +MS  EK K  S DP K+ R+ TS  N +KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y  E+R +  +I EL+SE LGL + Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E    M VN+YPPCP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG  K+  HRAVTN +  R S+   + P D   M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
             ++   P Y+   + ++ + +  R  D +  +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

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BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-66
Identity = 132/321 (41.12%), Postives = 188/321 (58.57%), Query Frame = 0


Query: 15  SVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFGFFQVINHGVS 74
           ++P  YV P  +RP   +V     IPV+DL++ DR  +V  + DA +  GFFQV+NHG+ 
Sbjct: 19  TMPGKYVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGID 78

Query: 75  KRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNCH 134
             ++     + ++F  +   EKAK  S DP K  R+ TS  N RKE  H WRD L  +C+
Sbjct: 79  AALIASVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF-NVRKETVHNWRDYLRLHCY 138

Query: 135 PLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGGEMSENPL-M 194
           PL ++V  WP NPP ++E++G YC E+R L   + E +SE LGL  GY+   + E    M
Sbjct: 139 PLHQFVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQEQHM 198

Query: 195 LVNHYPPCPNPSLTLGLSQHCDPSLITILF--QEVNGLQVLKDGQWIGVHPIDNAFVVNI 254
            VN+YP CP P LT GL  H DP+ +TIL    +V GLQVL DG+WI V+P   A V+NI
Sbjct: 199 AVNYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINI 258

Query: 255 GFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRAL 314
           G  LQ +SNGK ++  HRAV N+   R S+   + P + V + PAK LI + +P  YR  
Sbjct: 259 GDQLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNY 318

Query: 315 KFKDFQRNYLPRASDTKAVME 333
            + ++ + +  R  D +  +E
Sbjct: 319 TYDEYYKKFWSRNLDQEHCLE 338

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BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 124/328 (37.80%), Postives = 190/328 (57.93%), Query Frame = 0


Query: 13  VQSVPPDYVYPPEKRPG-NVVVPMANTIPVLDLST---RDRPLLVRKILDASQEFGFFQV 72
           V  VP +YV P   RP  + V    ++IP++DL      +R  ++ +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCWRDA 132
            NHGV +  +++     ++F   S +E+ K  S D  K+ R+ TS  N  KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 133 LIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGGEMS 192
           L  +C+P++ +++ WP  P  +REV   Y   +RALVL +LE +SE LGL +  +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 EN-PLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
           ++   M +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA--N 312
           F+VN+G  +QVISN K K+  HRAV N+ + R S+     P ++  + PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPRYRALKFKDFQRNYLPRASDTKAVME 333
           P  YR   + ++   +   A DT++ ++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCID 341

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BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot
Match: Q40062 (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=3)

HSP 1 Score: 223.0 bits (567), Expect = 5.1e-57
Identity = 122/309 (39.48%), Postives = 181/309 (58.58%), Query Frame = 0


Query: 15  SVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFGFFQVINHGVS 74
           S+P  +V+  +K P         ++P++DLS   R  + R IL+A +E GFFQV+NHGVS
Sbjct: 13  SLPESFVFASDKVP--PATKAVVSLPIIDLSC-GRDEVRRSILEAGKELGFFQVVNHGVS 72

Query: 75  KRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCWRDALIFNC- 134
           K+V+ +   + ++F  +  A+KA   S + +K  R++ S   Y       WRD L   C 
Sbjct: 73  KQVMRDMEGMCEQFFHLPAADKASLYSEERHKPNRLF-SGATYDTGGEKYWRDCLRLACP 132

Query: 135 HPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGGEMS-ENPL 194
            P+D  ++ WP  P   R+V+  +  + R +  E+L L+ EG+G+   Y  G++S  N +
Sbjct: 133 FPVDDSINEWPDTPKGLRDVIEKFTSQTRDVGKELLRLLCEGMGIQADYFEGDLSGGNVI 192

Query: 195 MLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNAFVVNI 254
           + +NHYP CPNP   LG   HCD +LIT+L    VNGL+V   G WI V P  NAFVVN 
Sbjct: 193 LNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNF 252

Query: 255 GFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEANPPRYRAL 314
           G  L+V++NG LK+ EHRA+TN+ ++R S+   + P  E  + PAK  +++ NPP YR  
Sbjct: 253 GQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTT 312

Query: 315 KFKDFQRNY 321
            F+DF R Y
Sbjct: 313 MFRDFMRIY 317

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BLAST of Cp4.1LG17g06100 vs. NCBI nr
Match: XP_023514953.1 (hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700 bits (1807), Expect = 1.04e-254
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339

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BLAST of Cp4.1LG17g06100 vs. NCBI nr
Match: KAG6593078.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688 bits (1776), Expect = 5.76e-250
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLSTRDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEK KECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

[Close]

BLAST of Cp4.1LG17g06100 vs. NCBI nr
Match: KAG7025486.1 (Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 687 bits (1773), Expect = 1.65e-249
Identity = 331/338 (97.93%), Postives = 333/338 (98.52%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLSTRDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGV +RVLEETTRIFKKFHAMSGAEKAKECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVPRRVLEETTRIFKKFHAMSGAEKAKECSRDPKKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

[Close]

BLAST of Cp4.1LG17g06100 vs. NCBI nr
Match: XP_022959666.1 (hyoscyamine 6-dioxygenase-like [Cucurbita moschata])

HSP 1 Score: 683 bits (1763), Expect = 5.52e-248
Identity = 329/338 (97.34%), Postives = 331/338 (97.93%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEK KECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

[Close]

BLAST of Cp4.1LG17g06100 vs. NCBI nr
Match: XP_023004374.1 (hyoscyamine 6-dioxygenase-like [Cucurbita maxima])

HSP 1 Score: 677 bits (1748), Expect = 1.03e-245
Identity = 327/339 (96.46%), Postives = 332/339 (97.94%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDY+YPPEKRPGNVVVPMAN IPV+DLSTRDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEKAKECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYCV MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYL  EMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGS+ S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

[Close]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL
Match: A0A6J1H5H6 (hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=3 SV=1)

HSP 1 Score: 683 bits (1763), Expect = 2.67e-248
Identity = 329/338 (97.34%), Postives = 331/338 (97.93%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMAN IPVLDLST DRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANAIPVLDLSTHDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEK KECSRDPNKSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKVKECSRDPNKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYC+ MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCIAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYLGGEMSENPLMLVNHYP CPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLGGEMSENPLMLVNHYPSCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHR VTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRVVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDR 338

[Close]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL
Match: A0A6J1KW36 (hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 SV=1)

HSP 1 Score: 677 bits (1748), Expect = 4.99e-246
Identity = 327/339 (96.46%), Postives = 332/339 (97.94%), Query Frame = 0


Query: 1   MGEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDAS 60
           MGEVKFVSNWEGVQSVPPDY+YPPEKRPGNVVVPMAN IPV+DLSTRDRPLLVRKILDAS
Sbjct: 1   MGEVKFVSNWEGVQSVPPDYIYPPEKRPGNVVVPMANAIPVVDLSTRDRPLLVRKILDAS 60

Query: 61  QEFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKE 120
           QEFGFFQVINHGVS+RVLEETTRIFKKFHAMSGAEKAKECSRDP KSCRVYTSSENYRKE
Sbjct: 61  QEFGFFQVINHGVSRRVLEETTRIFKKFHAMSGAEKAKECSRDPYKSCRVYTSSENYRKE 120

Query: 121 QTHCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGR 180
           QTHCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYCV MR LVLEILELVSEGLGLGR
Sbjct: 121 QTHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRTLVLEILELVSEGLGLGR 180

Query: 181 GYLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240
           GYL  EMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV
Sbjct: 181 GYLASEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGV 240

Query: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300
           HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI
Sbjct: 241 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 300

Query: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGS+ S
Sbjct: 301 NEANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSNCS 339

[Close]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL
Match: A0A0A0K5H3 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 608 bits (1568), Expect = 1.46e-218
Identity = 287/338 (84.91%), Postives = 312/338 (92.31%), Query Frame = 0


Query: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQ 61
           G  KFVSNWE V+SVP  YVYPPEKRPGN+VVPMA  IPV+DLS RDR LLVRKILDAS+
Sbjct: 4   GGAKFVSNWETVESVPESYVYPPEKRPGNIVVPMAKAIPVIDLSIRDRTLLVRKILDASK 63

Query: 62  EFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQ 121
           EFGFFQ+INHGVSK+V EET RIFK+FHAMSG EKAKECS+DPN+SCRVYTSSENY KEQ
Sbjct: 64  EFGFFQIINHGVSKKVSEETMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYTKEQ 123

Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRG 181
            HCWRDALIFNCHPL+KYVHFWPQ PPKYREVVGAYC+ MR LVLEILEL+SEGLGLG+G
Sbjct: 124 VHCWRDALIFNCHPLEKYVHFWPQKPPKYREVVGAYCMAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           Y GGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YFGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
           PIDNAFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE  MEPAKC+IN
Sbjct: 244 PIDNAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMIN 303

Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++S
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

[Close]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL
Match: A0A1S3BR20 (hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1)

HSP 1 Score: 604 bits (1558), Expect = 4.87e-217
Identity = 287/338 (84.91%), Postives = 311/338 (92.01%), Query Frame = 0


Query: 2   GEVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQ 61
           G+ KFVSNWE  +SVP  YVYPPEKRPGNVVVPMA  IPV+DLS  DR LLVRKIL AS+
Sbjct: 4   GDAKFVSNWETAESVPESYVYPPEKRPGNVVVPMAKAIPVIDLSICDRALLVRKILGASK 63

Query: 62  EFGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQ 121
           E GFFQ+INHGVSKRV E T RIFK+FHAMSG EKAKECS+DPN+SCRVYTSSENY+KEQ
Sbjct: 64  ESGFFQIINHGVSKRVSEATMRIFKEFHAMSGPEKAKECSKDPNRSCRVYTSSENYKKEQ 123

Query: 122 THCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRG 181
            HCWRDALIFNCHPL+KYVHFWPQNPPKYREVVGAYCV MR LVLEILEL+SEGLGLG+G
Sbjct: 124 IHCWRDALIFNCHPLEKYVHFWPQNPPKYREVVGAYCVAMRKLVLEILELMSEGLGLGKG 183

Query: 182 YLGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVH 241
           YLGGEMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ+VNGLQVLKDGQWIGV 
Sbjct: 184 YLGGEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQ 243

Query: 242 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLIN 301
           PID AFVVNIGFVLQ+I+NGKL+AAEHRAVTN+K SRQSLTYLVYPKDE TMEPAKC+IN
Sbjct: 244 PIDKAFVVNIGFVLQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMIN 303

Query: 302 EANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           EANPPRYR+L FKDFQRNYLPRA DTKAVM+ I +++S
Sbjct: 304 EANPPRYRSLNFKDFQRNYLPRAVDTKAVMQYIETNQS 341

[Close]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL
Match: A0A6J1CP90 (hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE=3 SV=1)

HSP 1 Score: 585 bits (1509), Expect = 1.37e-209
Identity = 271/337 (80.42%), Postives = 304/337 (90.21%), Query Frame = 0


Query: 3   EVKFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQE 62
           +V FVSNW GV+SVP  YVYPPEKRPGNV VP+ N IPV+DL+  DR  LV KILDASQE
Sbjct: 4   QVNFVSNWVGVESVPESYVYPPEKRPGNVAVPVENAIPVVDLTDHDRAFLVHKILDASQE 63

Query: 63  FGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQT 122
           FGFFQVINHGVSK ++EET R+F++FHAMSG EK +ECS+DPN+SCRVYTSSEN+ KE+T
Sbjct: 64  FGFFQVINHGVSKTLIEETMRVFEEFHAMSGPEKKRECSKDPNRSCRVYTSSENFVKEKT 123

Query: 123 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGY 182
           HCWRDALIFNCHPLDKYVHFWPQNP  YREVVGAYC+ MR LV+EILEL+SEGLGLG+GY
Sbjct: 124 HCWRDALIFNCHPLDKYVHFWPQNPSNYREVVGAYCIVMRKLVVEILELISEGLGLGKGY 183

Query: 183 LGGEMSENPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLKDGQWIGVHP 242
            G EMSENPL+LVNHYPPCPNPSLTLGLSQHCDPSLITILFQ++NGLQVLKDGQWIGVHP
Sbjct: 184 FGDEMSENPLLLVNHYPPCPNPSLTLGLSQHCDPSLITILFQQINGLQVLKDGQWIGVHP 243

Query: 243 IDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINE 302
           +DNAFVVNIGFVLQV+SNGKLKA EHRAV NAK+SRQS+TYLVYPKD+ TMEPAKC+INE
Sbjct: 244 VDNAFVVNIGFVLQVVSNGKLKAGEHRAVINAKMSRQSITYLVYPKDDATMEPAKCMINE 303

Query: 303 ANPPRYRALKFKDFQRNYLPRASDTKAVMECIGSDRS 339
           ANP  Y +L+FKDFQRNYLP A+DTKAV++ I SD S
Sbjct: 304 ANPSLYHSLQFKDFQRNYLPMAADTKAVIQYISSDHS 340

[Close]

BLAST of Cp4.1LG17g06100 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.5e-69
Identity = 139/334 (41.62%), Postives = 200/334 (59.88%), Query Frame = 0


Query: 5   KFVSNWEGVQSVPPDYVYPPEKRPGNVVVPMANTIPVLDLSTRDRPLLVRKILDASQEFG 64
           K +S      ++P +YV P   RP    V      P++DLS+ DR  L+++I  A   FG
Sbjct: 4   KLISTGFRHTTLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFG 63

Query: 65  FFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHC 124
           FFQVINHGV+K++++E   + ++F +MS  EK K  S DP K+ R+ TS  N +KE+ + 
Sbjct: 64  FFQVINHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF-NVKKEEVNN 123

Query: 125 WRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLG 184
           WRD L  +C+P+ KYV+ WP NPP ++E+V  Y  E+R +  +I EL+SE LGL + Y+ 
Sbjct: 124 WRDYLRLHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMK 183

Query: 185 GEMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQE--VNGLQVLKDGQWIGVH 244
             + E    M VN+YPPCP P LT GL  H DP+ +TIL Q+  V GLQ+L DGQW  V+
Sbjct: 184 KVLGEQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVN 243

Query: 245 PIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCL-- 304
           P  +AFV+NIG  LQ +SNG  K+  HRAVTN +  R S+   + P D   M PAK L  
Sbjct: 244 PHPDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWE 303

Query: 305 -INEANPPRYRALKFKDFQRNYLPRASDTKAVME 333
             ++   P Y+   + ++ + +  R  D +  +E
Sbjct: 304 AEDDETKPVYKDFTYAEYYKKFWSRNLDQEHCLE 336

[Close]

BLAST of Cp4.1LG17g06100 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 124/328 (37.80%), Postives = 190/328 (57.93%), Query Frame = 0


Query: 13  VQSVPPDYVYPPEKRPG-NVVVPMANTIPVLDLST---RDRPLLVRKILDASQEFGFFQV 72
           V  VP +YV P   RP  + V    ++IP++DL      +R  ++ +   A    GFFQ+
Sbjct: 15  VDHVPSNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQI 74

Query: 73  INHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCWRDA 132
            NHGV +  +++     ++F   S +E+ K  S D  K+ R+ TS  N  KE+   WRD 
Sbjct: 75  KNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF-NVSKEKVSNWRDF 134

Query: 133 LIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGGEMS 192
           L  +C+P++ +++ WP  P  +REV   Y   +RALVL +LE +SE LGL +  +   + 
Sbjct: 135 LRLHCYPIEDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIG 194

Query: 193 EN-PLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGVHPIDNA 252
           ++   M +N+YP CP P LT GL  H D +LIT+L Q EV+GLQV KDG+WI V+P+ N 
Sbjct: 195 KHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNT 254

Query: 253 FVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA--N 312
           F+VN+G  +QVISN K K+  HRAV N+ + R S+     P ++  + PA+ LINE   +
Sbjct: 255 FIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDS 314

Query: 313 PPRYRALKFKDFQRNYLPRASDTKAVME 333
           P  YR   + ++   +   A DT++ ++
Sbjct: 315 PAIYRNFTYAEYFEKFWDTAFDTESCID 341

[Close]

BLAST of Cp4.1LG17g06100 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 222.6 bits (566), Expect = 4.7e-58
Identity = 121/333 (36.34%), Postives = 189/333 (56.76%), Query Frame = 0


Query: 7   VSNWEGVQSVPPDYVYPPEKRPG-NVVVPMANTIPVLDLST---RDRPLLVRKILDASQE 66
           VS++     +P +YV P   RP  + V    ++IP++DL      +R ++V+++  A   
Sbjct: 11  VSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQLASACST 70

Query: 67  FGFFQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQT 126
           +GFFQ+ NHGV    + +   + ++F     +E+ K  S DP K+ R+ TS  N   ++ 
Sbjct: 71  YGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-NVGADKV 130

Query: 127 HCWRDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGY 186
             WRD L  +C P++ ++  WP +P  +REV   Y   +RALVL +LE +SE LGL   +
Sbjct: 131 LNWRDFLRLHCFPIEDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISESLGLESDH 190

Query: 187 LGGEMSENPL-MLVNHYPPCPNPSLTLGLSQHCDPSLITILFQ-EVNGLQVLKDGQWIGV 246
           +   + ++   M  N+YPPCP P LT GL  H DP++IT+L Q +V+GLQV KD +W+ V
Sbjct: 191 ISNILGKHAQHMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQVSGLQVFKDDKWVAV 250

Query: 247 HPIDNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLI 306
            PI N F+VNIG  +QVISN K K+  HRAV N +  R S+    +P  +  + PA  L+
Sbjct: 251 SPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTDAVIGPAHELV 310

Query: 307 NEANP-PRYRALKFKDFQRNYLPRASDTKAVME 333
           NE +    YR   F ++   +  R+  T + ++
Sbjct: 311 NEQDSLAIYRTYPFVEYWDKFWNRSLATASCLD 342

[Close]

BLAST of Cp4.1LG17g06100 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.3e-52
Identity = 118/318 (37.11%), Postives = 171/318 (53.77%), Query Frame = 0


Query: 12  GVQSVPPDYVYPPEKRP--GNVVVPMANTIPVLDLSTRDRPLL----VRKILDASQEFGF 71
           GV  VP  YV PP +RP  G+ +     T+PV+DLS   +P L    + +I  A +EFGF
Sbjct: 23  GVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGF 82

Query: 72  FQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCW 131
           FQVINHG+   V+ +      +F  +   EK    S + ++  R Y +S N+  ++ H W
Sbjct: 83  FQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVR-YGTSLNHSTDRVHYW 142

Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGG 191
           RD +    HPL K++  WP NPP Y++ VG Y      L  +++E +SE LGL + YL  
Sbjct: 143 RDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
           E+ E + +M VN YP CP P + LG+  H D S +TIL Q   GLQ++  +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIMDCNKNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
           + A +V +G  ++V+SNG  K+  HR   N ++ R S   L        + PA  L+N  
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLHKKISPAPKLVNPN 322

Query: 312 NPPRYRALKFKDFQRNYL 322
           N P Y    F DF  NY+
Sbjct: 323 NAPAYGEFSFNDF-LNYI 338

[Close]

BLAST of Cp4.1LG17g06100 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 5.2e-49
Identity = 116/325 (35.69%), Postives = 167/325 (51.38%), Query Frame = 0


Query: 12  GVQSVPPDYVYPPEKRP--GNVVVPMANTIPVLDLSTRDRPLL----VRKILDASQEFGF 71
           G   VP  Y+ PP +RP  G  +      +PV+DLS    PLL    + +I  A + FGF
Sbjct: 23  GDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFLHDPLLRLCVIHEIELACKGFGF 82

Query: 72  FQVINHGVSKRVLEETTRIFKKFHAMSGAEKAKECSRDPNKSCRVYTSSENYRKEQTHCW 131
           FQVINHG+S  V+++      +F  +   EK    S +  +  R Y +S N+  ++ H W
Sbjct: 83  FQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVR-YGTSINHSTDRVHYW 142

Query: 132 RDALIFNCHPLDKYVHFWPQNPPKYREVVGAYCVEMRALVLEILELVSEGLGLGRGYLGG 191
           RD +    HPL  +++ WP NPP Y+E VG Y      L  +++E +SE LGL + YL  
Sbjct: 143 RDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISESLGLEKNYLQE 202

Query: 192 EMSE-NPLMLVNHYPPCPNPSLTLGLSQHCDPSLITILFQEVNGLQVLK-DGQWIGVHPI 251
           E+ E + +M VN YP CP P + LG+  H D   +TIL Q   GLQ+   +  W+ V  I
Sbjct: 203 EIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKDCNNNWVCVPYI 262

Query: 252 DNAFVVNIGFVLQVISNGKLKAAEHRAVTNAKISRQSLTYLVYPKDEVTMEPAKCLINEA 311
           + A +V +G  ++V+SNG  K+  HR   N    R S   L        + PA  L+NE 
Sbjct: 263 EGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHKKISPATQLVNEN 322

Query: 312 NPPRYRALKFKDFQRNYLPRASDTK 329
            P  Y    F DF  +Y+ R   T+
Sbjct: 323 KPAAYGEFSFNDF-LDYISRNDITQ 345

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g06100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P24397	1.7e-68	42.17	Hyoscyamine 6-dioxygenase OS=Hyoscyamus niger OX=4079 GN=H6H PE=1 SV=1	[more]
Q9FLV0	4.9e-68	41.62	Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S...	[more]
Q8W2X5	3.5e-66	41.12	Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1...	[more]
Q9ZSA7	2.1e-58	37.80	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]
Q40062	5.1e-57	39.48	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare OX=4513 GN=IDS3 PE=1 SV=...	[more]

BLAST of Cp4.1LG17g06100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514953.1	1.04e-254	100.00	hyoscyamine 6-dioxygenase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6593078.1	5.76e-250	97.93	Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7025486.1	1.65e-249	97.93	Hyoscyamine 6-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022959666.1	5.52e-248	97.34	hyoscyamine 6-dioxygenase-like [Cucurbita moschata]	[more]
XP_023004374.1	1.03e-245	96.46	hyoscyamine 6-dioxygenase-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG17g06100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H5H6	2.67e-248	97.34	hyoscyamine 6-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111460677 PE=...	[more]
A0A6J1KW36	4.99e-246	96.46	hyoscyamine 6-dioxygenase-like OS=Cucurbita maxima OX=3661 GN=LOC111497716 PE=3 ...	[more]
A0A0A0K5H3	1.46e-218	84.91	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G...	[more]
A0A1S3BR20	4.87e-217	84.91	hyoscyamine 6-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103492610 PE=3 SV=1	[more]
A0A6J1CP90	1.37e-209	80.42	hyoscyamine 6-dioxygenase-like OS=Momordica charantia OX=3673 GN=LOC111013466 PE...	[more]

BLAST of Cp4.1LG17g06100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24530.1	3.5e-69	41.62	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10490.1	1.5e-59	37.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G10500.1	4.7e-58	36.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G44800.1	2.3e-52	37.11	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G60290.1	5.2e-49	35.69	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 3..327 e-value: 2.4E-88 score: 298.6
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 191..285 e-value: 7.8E-23 score: 80.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 39..145 e-value: 1.7E-19 score: 70.7
None	No IPR available	PANTHER	PTHR47991:SF31	1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE	coord: 3..333
None	No IPR available	PANTHER	PTHR47991	OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASE	coord: 3..333
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..322
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 188..287 score: 12.406347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g06100.1	Cp4.1LG17g06100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding