Cp4.1LG17g05670 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG17g05670
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRegulator of Vps4 activity in the MVB pathway protein
LocationCp4.1LG17: 4634399 .. 4636870 (+)
RNA-Seq ExpressionCp4.1LG17g05670
SyntenyCp4.1LG17g05670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTGGCAGTTCTATTTTCTTCTTCTTCATCTTCTTACACAGCTGGGATTTTCCGGTATCTTTTTGGCGGGTTTTCGATTCTTCTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTCTTCTTTTATAATAAACCCTTTTTTTTTGGAGTTCATTTTTGTCGGTGAGCTTCTCTGTTTCTCTTGCACTGTCGTTTCTGTGTTGTATTGTTCATGTTCATCTTCCATTTGGCTGATTATTACTCGTTCCTCCTTTTGATGTTTTGTAAACATTCTTCAATGGCGGATGTGGGATTATTCCATTTTCTTCATCCCTTCTTTTAATTGTTTTTGGAATCTTGGTTGTGGGTTTCTTGGGATTTTCATTCTCCATTGCCTGATTCTTCGGTGTTCTTCCTCGTTTCAGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGGTATTGATTCTGATTCTGATTCTGATTCCGATTCCGATTCTGATTTTTATGGGTTTCTTTTGATTGATTCTTTCTTTTATTGTAATGTAGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGTTAGTTCTTCTTTATGTCTTGGATTGGATTCTTAATCTCTCCGAAACCCATAATTTTAATATCTGTCTTCTGTTGTGATTGTTCTTAAATCCTTTGTGTTTGGATTGACTGTAAGAGAATTTCTGGTTCATATTCCCTGATTTTGGTCGTGCTTGTTGTGCTAATGGTGAAATAGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTGTCTGTCTTGATCAAACAGAGGTAGTAGATATCATGATTTTTGGAACATGTCTGTGTTCTTAGGCTTCAATTTGTTCAAATCAGTTTAAATGTGGTTTCCATGTTTTTGTTTCTGTTCTAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCGGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGGTTGTTCTTGGTAGCTTAAAAACTCGGTTGTTTCGTTGGAGGGTCGTCCTAAAACTTGGTTTAATTCTTGTTTTTCCTTTTAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAAGTGAGTTTGTGTGCAGTTCTGTTGTTGTGTGTTGATGAACTCTCTTCATTCTATTGATGGTTTTATGGTCAATCTTGTTGCAGCCTGATGTTGTTCCTGTGCACGGAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCAACCAAGTAGAAAACGATGAACGTAATAAGCAGCAACCCGAGCCAACCTTAGAAGAGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATCGACAATGTCGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAGGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGTTAACTGAGCTAGAGAACAAGCATCATATTAGAACCAATTCGTTCGTTCATCCTAAGCTCCCGGACTACGACGAACTCGCAGCTCGGTTTGCAGCCCTCAAGGAAGATAAATAAAAGGTGTCGGTATCAGACGCTCGAGAAAATTATCGATCGAACATGGTTGGCATGACATTAACAAGGAGACTCCATCCTAAAGGAAGATTCTTTGCTTCGAAACTAAACCAACAGGACACTTGCAATTCTCTAATCATTTCTTACAACCTACCTGTACTTTATAACTTNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATATA

mRNA sequence

CGTGGCAGTTCTATTTTCTTCTTCTTCATCTTCTTACACAGCTGGGATTTTCCGGTATCTTTTTGGCGGGTTTTCGATTCTTCTTCTTCTTCTTTCTCCTCCATTATATCTCTCTCGCTTTCGTTCTTCTTTTATAATAAACCCTTTTTTTTTGGAGTTCATTTTTGTCGCCCTCTAAATTTCTGTTTTCTTTCGGATTTCTTCGGTTTCAAAATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTGTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCGGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCTGTGCACGGAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCAACCAAGTAGAAAACGATGAACGTAATAAGCAGCAACCCGAGCCAACCTTAGAAGAGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATCGACAATGTCGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAGGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGTTAACTGAGCTAGAGAACAAGCATCATATTAGAACCAATTCGTTCGTTCATCCTAAGCTCCCGGACTACGACGAACTCGCAGCTCGGTTTGCAGCCCTCAAGGAAGATAAATAAAAGGTGTCGGTATCAGACGCTCGAGAAAATTATCGATCGAACATGGTTGGCATGACATTAACAAGGAGACTCCATCCTAAAGGAAGATTCTTTGCTTCGAAACTAAACCAACAGGACACTTGCAATTCTCTAATCATTTCTTACAACCTACCTGTACTTTATAACTTNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATATA

Coding sequence (CDS)

ATGTTCGACATGTTCTTGAAGCCGAAGTACTACACGAAATGCAAATCTTGCTTAAAGCTGTTGAAGTCGCGGCTGGATGCAATTAGGAAGAAGAAGAATGCGGTTCTTAAGTATTTGAAGAGCGACATTGTTGAACTTCTCAAGAGTCGTCTCGATTACAATGCTTACAACAGGGCTGAAGGGTTTCTTGTTGAGCGAAATGTGTTGAGATGTTATGAGCTAATTGATGAGTTTTGTGGGACTATCTCCAATCAACTGTCTGTCTTGATCAAACAGAGCGAATGCCCTGATGAATGCAACGAAGCAGTCGCGTCGTTGATCTACGCTGCAGCGAGATTCGCGGACTTGCCTGAATTACGAGAGCTTCGAAACTTCTTTACTGGGAAATATGGATGTTCCTTCGAACCATTTACCAGCATAGAGCTTATCGAGAAGTCGAGGGCGACGATTCAATCGAAAGAGATAAAGCTTCAACTACTTCAGGAGATTGCTCAGGAATCCGCCATTGATTGGAACTCCAAAGCTCTTGAACAACAATTGTATATTCCTCCCCCAAATGAACCTGATGTTGTTCCTGTGCACGGAAGGAAGAAGAGCTCGGTCGGAAAAAGGGACAGCAGCGACGACGAATCCATGTTCGATATTAGAAGTGAAGGCAACACGACCGAGACCTCGACCGTAGATAGTAGTACTGATCAAGATGTCCGAAAAGGAGGTTCCGAGGATGAAGTAGAGGATCAGACACCGTTCTACCTTAGAGTTATCCGACCTCCTTACCTTAAAACGAAACCAACCAACAAGGAAACAATGGAAGAAGCTCGAAAAGCTACCAACCAAGTAGAAAACGATGAACGTAATAAGCAGCAACCCGAGCCAACCTTAGAAGAGAAACCGAAACCGAAATCAGTCAGGCGAAGAAATGTGAAACTACAGCCTACAATGGAGATCAAGATCGACAATGTCGGGAATTCGGGAAACGATATGAGTTCGAGTCGAAACAAAGGTAAAGAAGCCATGAAAGGAGAAGGGGATGATGATGAGGAGGAGGAGAAGATGTTAGATGGGCTGTTGATGCATTACAGTAAGAAAAAACCAAGCCATGAATCAAACGAGGTCAGGGCAGTGTCACTGTCCATAGACTCAACTGAGCCAACTGAGTTAACTGAGCTAGAGAACAAGCATCATATTAGAACCAATTCGTTCGTTCATCCTAAGCTCCCGGACTACGACGAACTCGCAGCTCGGTTTGCAGCCCTCAAGGAAGATAAATAA

Protein sequence

MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAALKEDK
Homology
BLAST of Cp4.1LG17g05670 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 7.3e-13
Identity = 47/181 (25.97%), Postives = 91/181 (50.28%), Query Frame = 0

Query: 13  KCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRCY 72
           K K  LKL  SR+  ++ KK  +++  K ++ ELL+ + + +A  R E  + +  ++ C+
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 73  ELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG- 132
           ++I+  C  +  +++++   +E P E  E++ +L+Y++ R   +PEL +++N    KYG 
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 133 -------CSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYIPP 186
                  C      + +++ K         I  Q L EIA++  +DW         Y PP
Sbjct: 132 GLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDWCGSD-----YPPP 186

BLAST of Cp4.1LG17g05670 vs. NCBI nr
Match: XP_023514654.1 (uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 810 bits (2091), Expect = 3.80e-295
Identity = 423/423 (100.00%), Postives = 423/423 (100.00%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300

Query: 301 KSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLMHY 360
           KSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLMHY
Sbjct: 301 KSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLMHY 360

Query: 361 SKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAALK 420
           SKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAALK
Sbjct: 361 SKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAALK 420

Query: 421 EDK 423
           EDK
Sbjct: 421 EDK 423

BLAST of Cp4.1LG17g05670 vs. NCBI nr
Match: XP_022964044.1 (uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 779 bits (2011), Expect = 6.37e-283
Identity = 411/425 (96.71%), Postives = 416/425 (97.88%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVH RKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDERNKQQ EPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           K  SVRRRNVKLQPT+EIKI NV NSGND+SSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHH RTNSFVHPKLPDYD+LAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 423
           LKEDK
Sbjct: 421 LKEDK 425

BLAST of Cp4.1LG17g05670 vs. NCBI nr
Match: KAG6593450.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 770 bits (1988), Expect = 1.82e-279
Identity = 409/425 (96.24%), Postives = 413/425 (97.18%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVH RKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDERNKQQ EPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           K  SVRRRNVKLQPT+EIKI NV NSGND+SSSRNKGKEAMKGEGDDDEEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPT   ELENKHH RTNSFVHPKLPDYD+LAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPT---ELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 423
           LKEDK
Sbjct: 421 LKEDK 422

BLAST of Cp4.1LG17g05670 vs. NCBI nr
Match: XP_023000359.1 (uncharacterized protein LOC111494616 [Cucurbita maxima])

HSP 1 Score: 750 bits (1937), Expect = 1.11e-271
Identity = 402/426 (94.37%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+H RKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDE  KQQPEPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEE-KMLDGLL 360
           K  SVRRRNVKLQPTMEIKID+VGNSGNDMSSSRNKGKEAMKGEGDD+EEEE KMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPT   ELENK H RTNSFVHP LPDYDELAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPT---ELENKRHTRTNSFVHPNLPDYDELAARFA 420

Query: 421 ALKEDK 423
           ALKEDK
Sbjct: 421 ALKEDK 423

BLAST of Cp4.1LG17g05670 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 513 bits (1322), Expect = 1.96e-177
Identity = 308/478 (64.44%), Postives = 345/478 (72.18%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD+IRKKK AVLKYLK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCY LIDEFCGTIS+Q+ VL K+SECP+EC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYLLIDEFCGTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR+ FT KYG SF  FT+ ELIEKSRAT Q+KE+K+QLLQEIAQ+SAIDWNSKALEQQL
Sbjct: 121 ELRSLFTEKYGSSFGSFTNKELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NEP                 VPVH RK  S  K+D+SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNEPAGERSATIKRNKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKET-MEEARKATNQVEND 300
           ST DS TDQDV KG SEDEVEDQ PFYLR + PPYLKTKP   E  +EE++K T    ND
Sbjct: 241 STGDS-TDQDVHKGISEDEVEDQKPFYLRFVPPPYLKTKPIKTEANVEESQKVT-VGNND 300

Query: 301 ERNKQQPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVGNSGND----MSSSRNKGKEAM 360
           E NKQ P+   EEKPKP+SVRRRNVK QP  +I ID+ G+S  D     S S+NKGKE M
Sbjct: 301 ESNKQHPKSPTEEKPKPRSVRRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETM 360

Query: 361 KGE----GDDDEEEEKMLDGLLMHYSKKKPSHESNE------------------------ 420
            GE    GDD   EE++LDGLLM YS+KK S ESN                         
Sbjct: 361 IGEERGAGDD---EERVLDGLLMQYSRKKTSQESNRGKGNLKPQRQQEKDNAEPQKPKST 420

BLAST of Cp4.1LG17g05670 vs. ExPASy TrEMBL
Match: A0A6J1HLZ1 (uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC111464187 PE=3 SV=1)

HSP 1 Score: 779 bits (2011), Expect = 3.08e-283
Identity = 411/425 (96.71%), Postives = 416/425 (97.88%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKN VLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNTVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVPVH RKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPVHERKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDERNKQQ EPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDERNKQQSEPTSEEKPKP 300

Query: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEEKMLDGLLM 360
           K  SVRRRNVKLQPT+EIKI NV NSGND+SSSRNKGKEAMKGEGDD+EEEEKMLDGLLM
Sbjct: 301 KPKSVRRRNVKLQPTVEIKIGNVENSGNDISSSRNKGKEAMKGEGDDEEEEEKMLDGLLM 360

Query: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFAA 420
           HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHH RTNSFVHPKLPDYD+LAARFAA
Sbjct: 361 HYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHTRTNSFVHPKLPDYDKLAARFAA 420

Query: 421 LKEDK 423
           LKEDK
Sbjct: 421 LKEDK 425

BLAST of Cp4.1LG17g05670 vs. ExPASy TrEMBL
Match: A0A6J1KJN9 (uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616 PE=3 SV=1)

HSP 1 Score: 750 bits (1937), Expect = 5.38e-272
Identity = 402/426 (94.37%), Postives = 408/426 (95.77%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG ISNQLSVLIKQSECPDE  EAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRNFFTGKYGCSFEPFTSIELIEKSRATIQ+KEIKLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240
           YIPPPNEPDVVP+H RKK SVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS
Sbjct: 181 YIPPPNEPDVVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGS 240

Query: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERNKQQPEPTLEEKPKP 300
           EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKAT+QVENDE  KQQPEPT EEKPKP
Sbjct: 241 EDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDECKKQQPEPTSEEKPKP 300

Query: 301 K--SVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEEEE-KMLDGLL 360
           K  SVRRRNVKLQPTMEIKID+VGNSGNDMSSSRNKGKEAMKGEGDD+EEEE KMLDGLL
Sbjct: 301 KPKSVRRRNVKLQPTMEIKIDDVGNSGNDMSSSRNKGKEAMKGEGDDEEEEEEKMLDGLL 360

Query: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFVHPKLPDYDELAARFA 420
           MHYSKKKPSHESNEVRAVSLSIDSTEPT   ELENK H RTNSFVHP LPDYDELAARFA
Sbjct: 361 MHYSKKKPSHESNEVRAVSLSIDSTEPT---ELENKRHTRTNSFVHPNLPDYDELAARFA 420

Query: 421 ALKEDK 423
           ALKEDK
Sbjct: 421 ALKEDK 423

BLAST of Cp4.1LG17g05670 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 509 bits (1310), Expect = 4.13e-176
Identity = 306/467 (65.52%), Postives = 343/467 (73.45%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMFLKPK+YTKCKSC+K+ K+RLD IRKKKNAVLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCGTISNQ+ VL K+SECPDEC EAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELRN FT KYG SF  FT+ E IEKSRAT Q+KE+K+QLLQEIAQE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YIPPP-NEPD---------------VVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTET 240
           Y PPP NE D                VPV+ RK +S   +++SD+ES+FD RSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETME-EARKATNQVEND 300
           ST DS TDQD+ KG SEDEVEDQ PF  R ++PPYLKTKP   E  E E RK T  +END
Sbjct: 241 STGDS-TDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ERNKQQPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVGNSGNDM----SSSRNKGKEAM 360
           E N   P+   EEKPKP+SVRRRNVK QP  +I ID+VG+S  D+    SSSRNKGKE M
Sbjct: 301 ESNN--PKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETM 360

Query: 361 KGE--GDDDEEEEKMLDGLLMHYSKKKPSHESNE-----------------------VRA 420
            GE  G  D+EE ++LDGLLM YSKKK + ES                          RA
Sbjct: 361 IGEEKGARDDEE-RVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRPTTRA 420

BLAST of Cp4.1LG17g05670 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 7.32e-173
Identity = 293/470 (62.34%), Postives = 327/470 (69.57%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFDMF KPK++TKCKSC+KLLK RLD IRKKKNAVLK+LK+DI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF GTI NQLSVL KQSECPDEC EAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR+ FT KYG SFE FT+ ELIEK  A   +KE+KLQLLQEIAQES I+WNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YIPPPNEPDV--------------VPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETST 240
           Y PP N PD               +PV  RK S + K+D+SDD+S+FD RSE N TETST
Sbjct: 181 YTPPQNTPDEEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETST 240

Query: 241 VDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVENDERN 300
            DSSTDQDV+KG  EDEVED+ PFYLR I PPYLK+KP+ KE   E              
Sbjct: 241 GDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE-------------- 300

Query: 301 KQQPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVGNSGND----MSSSRNKGKEAMKGE 360
               +P +EEKPKP+SVRRRN KL+PT +I I NV NS ND     SSSRNKGKE M GE
Sbjct: 301 ----KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMGE 360

Query: 361 GDDDEEEEKMLDGLLMHYSKKKPSHESNEV------------------------------ 420
                EEE+MLDGLLMHYSKKK + ESN                                
Sbjct: 361 EKGAGEEERMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQDEPQRTKNSKAEEDLSVP 420

BLAST of Cp4.1LG17g05670 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 493 bits (1269), Expect = 6.20e-170
Identity = 289/461 (62.69%), Postives = 332/461 (72.02%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSC+K+ K+RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCGTI   +SVL K+SECPDEC EAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
            LR+ FT +YG SF  FT+ EL+EK RA  Q+KE KLQLLQEIAQESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YIPPPNEPDV---------------VPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETS 240
           Y+PP NE D                VPV+GR  +S  K+D+SDD+S+FD RSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TVDSSTDQDVRKGG-SEDEVEDQTPFYLRVIRPPYLKTKPTNKE-TMEEARKATNQVEND 300
             DSSTDQDV KG  SEDEVEDQ PFYLR I PPYLKTKPT KE  +EE +  T +  ND
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDTEENRND 300

Query: 301 ERNKQQPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVGNSGND----MSSSRNKGKEAM 360
                 P+  +EEKPKP+SVRRR+ K QP   I ID+ G S  D    +SSSRNKGKEAM
Sbjct: 301 ------PKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAM 360

Query: 361 KGEGDD-DEEEEKMLDGLLMHYSKKKPSHESNE------------------VRAVSLSID 420
            GE    +++EE+MLDGLL  YSKKK   E  +                   +++SL   
Sbjct: 361 TGEEKGVEDDEERMLDGLLKLYSKKKTRQEDKDNAEPQRPKNTRTEDAYVPTKSLSLPPP 420

BLAST of Cp4.1LG17g05670 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.5e-61
Identity = 144/355 (40.56%), Postives = 215/355 (60.56%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD   KPK+YTKCKS +K+ K+R+D +++KKN+V KYLK+DIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           +LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YIPP--------PNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDSS 240
           Y PP          EP     +   + ++ KR S       D   + N +   TS  DS 
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDSM 240

Query: 241 TDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATNQVENDERNKQQ 300
           +       G ED+ E   PFY R + P PY K K   +E++ E    ++ ++ +      
Sbjct: 241 STSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE------ 300

Query: 301 PEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVG---NSGNDMSSSRNKGKEAMKG 342
             PT   KPKP+SVRRR     P  E + + +     S N M +S + GK + +G
Sbjct: 301 -SPT-GGKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 346

BLAST of Cp4.1LG17g05670 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.2e-37
Identity = 106/296 (35.81%), Postives = 165/296 (55.74%), Query Frame = 0

Query: 60  EGFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  +++ +S+L K   CPDEC EA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 179
           R+LR+ F  +YG + + F + E +E+ +A   SKE+K++LLQEIA+E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYIPP--------PNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTT--ETSTVDS 239
           LY PP          EP     +   + ++ KR S       D   + N +   TS  DS
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEKTMEKRSSLSFHGREDSMDDSNKSIMSTSEEDS 225

Query: 240 STDQDVRKGGSEDEVEDQTPFYLRVIRP-PYLKTKPTNKETMEEARKATNQVENDERNKQ 299
            +       G ED+ E   PFY R + P PY K K   +E++ E    ++ ++ +     
Sbjct: 226 MSTSGSCMTGPEDDPE-MKPFYYRFMTPAPYTKPKIEKQESLPEKMTTSHLMDTE----- 285

Query: 300 QPEPTLEEKPKPKSVRRRNVKLQPTMEIKIDNVG---NSGNDMSSSRNKGKEAMKG 342
              PT   KPKP+SVRRR     P  E + + +     S N M +S + GK + +G
Sbjct: 286 --SPT-GGKPKPRSVRRRFANPPPEAETEGEALKLKVESKNMMRASESMGKASGEG 332

BLAST of Cp4.1LG17g05670 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 144.4 bits (363), Expect = 2.0e-34
Identity = 74/180 (41.11%), Postives = 118/180 (65.56%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFD FL   +  K K  +KL K+R+D +R+K+NA +K+LK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
           G L E   L   + +++ C  +  QLS + K  ECP++C EA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQL 180
           ELR  F  KY  S   F + EL+E   +   S E K++L++++A E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180


HSP 2 Score: 28.9 bits (63), Expect = 1.2e+01
Identity = 43/156 (27.56%), Postives = 68/156 (43.59%), Query Frame = 0

Query: 236 RKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATN---QVENDERNKQQPEP 295
           RK    +    ++PFY + + PPY+K K   K   E+A    N   + + +E N      
Sbjct: 502 RKVEERETERMKSPFY-KSLPPPYVK-KSIAKARHEKAEALDNPKARFDGEEGNHPDNGK 561

Query: 296 TLEEKPKPKSVRRRNVKLQPTMEIKID-----NVGNSGNDMSSSRNKGKEAMKG-----E 355
            +    +        V       +K       ++  SG D   S  + + + KG     +
Sbjct: 562 NVYGAERRNGAGHHEVNDIDNASLKRQTNRRKHIVESGGDDHISSRRRENSRKGLQVLID 621

Query: 356 GDDDEEEEKMLDGLLMHYSKKKPSHESNEVRAVSLS 379
            D+ + EEKM+D LLMHYSKK  S+E + V+  S S
Sbjct: 622 EDEKDSEEKMMDKLLMHYSKKPSSYEKDNVQEESKS 655

BLAST of Cp4.1LG17g05670 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 139.0 bits (349), Expect = 8.5e-33
Identity = 98/304 (32.24%), Postives = 164/304 (53.95%), Query Frame = 0

Query: 1   MFDMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+Y K KS    +K R+D +R+K+ A+++  K+DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  IS  LS+++K+ ECP+EC EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNFFTGKYGCSFEPFTSIELIEKSR-ATIQSKEIKLQLLQEIAQESAIDWNSKALEQQ 180
           +LR  FT ++G       + EL+EK+      S+E+K+Q ++++A E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LYIPPPNEPDVVPVHGRKKSSVG----------KRDSSDDESM------FDIRSEGNTTE 240
           L      E   + +  + ++               + SDDES+       D  S+G+ + 
Sbjct: 181 LL----RETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVLSESWRIDSLSKGSLSS 240

Query: 241 TSTVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVEND 288
           +S+  SS+ + V    S  + + +      +I  P  K    N E  +E ++   ++ + 
Sbjct: 241 SSSSSSSSLRRV----SVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEKRKIADQ 296

BLAST of Cp4.1LG17g05670 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 109.0 bits (271), Expect = 9.5e-24
Identity = 114/441 (25.85%), Postives = 205/441 (46.49%), Query Frame = 0

Query: 12  TKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRC 71
           +KCK+  K+  +R+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGTISNQLSVLIKQSECPDECNEAVASLIYAAARFADLPELRELRNFFTGKYG 131
            E+I+ FC  I ++L+++ KQ +CP +  E +ASLI+AA R +++PEL +LR+ F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 CSF-------EPFTSIE--LIEKSRATIQSKEIKLQLLQEIAQESAIDWNSKALEQQLYI 191
             F        P   +   LI+K        E KL++++EIA+E  +DW++   EQ+L +
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQEL-L 208

Query: 192 PPPNEPDVVPVHGRKKSSVGKRDSSDDESMFDIRSEGNTTETSTVDSSTDQDVRKGGSED 251
            P  E    P      SS+    ++ +E +   ++   +T + ++++          +  
Sbjct: 209 KPQEESIDGPRKFVSASSLPVNRAAINEPIDPTKAVPRSTSSMSINTHYHDTESAAEAAT 268

Query: 252 EVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATNQVEND----------ERNKQQPEP 311
           E+  Q     +V      +   +NKE    +  +T+Q ++            R + +   
Sbjct: 269 ELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDHHHHHPGSRRQSRDSE 328

Query: 312 TLEEKPKP----KSVRRRNVKLQPTMEIKIDNVGNSGNDMSSSRNKGKEAMKGEGDDDEE 371
           T     KP    + + RR+    P +     N  +   + +++  + KE M+     +  
Sbjct: 329 TSSYYAKPGAENRGMGRRHSYNNPGI-----NESDYEEEYTNTEAEAKETMRRRHSYNPR 388

Query: 372 E------EKMLDGLLMHYSKKKPSHESNEVRAVSLSIDSTEPTELTELENKHHIRTNSFV 424
                   ++      +Y ++    E ++ R  SL  +   P      E++    +   V
Sbjct: 389 SVPPPATSEIKFDESDYYEEETEPDEPSQGRVSSLPPNRAPPQAPQSGESRQD-SSGHQV 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q54I397.3e-1325.97IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023514654.13.80e-295100.00uncharacterized protein LOC111778886 [Cucurbita pepo subsp. pepo][more]
XP_022964044.16.37e-28396.71uncharacterized protein LOC111464187 [Cucurbita moschata] >KAG7025791.1 IST1-lik... [more]
KAG6593450.11.82e-27996.24IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023000359.11.11e-27194.37uncharacterized protein LOC111494616 [Cucurbita maxima][more]
XP_038897244.11.96e-17764.44uncharacterized protein LOC120085370 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HLZ13.08e-28396.71uncharacterized protein LOC111464187 OS=Cucurbita moschata OX=3662 GN=LOC1114641... [more]
A0A6J1KJN95.38e-27294.37uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616... [more]
A0A1S3CGT54.13e-17665.52uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=... [more]
A0A6J1CWS87.32e-17362.34vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 ... [more]
A0A6J1GMM16.20e-17062.69uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC1114558... [more]
Match NameE-valueIdentityDescription
AT1G79910.12.5e-6140.56Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G79910.21.2e-3735.81Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G32350.12.0e-3441.11Regulator of Vps4 activity in the MVB pathway protein [more]
AT1G52315.18.5e-3332.24Regulator of Vps4 activity in the MVB pathway protein [more]
AT4G35730.19.5e-2425.85Regulator of Vps4 activity in the MVB pathway protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 266..286
NoneNo IPR availableCOILSCoilCoilcoord: 143..163
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..349
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 265..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 317..332
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..216
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 234..249
NoneNo IPR availablePANTHERPTHR12161:SF60REGULATOR OF VPS4 ACTIVITY IN THE MVB PATHWAY PROTEINcoord: 9..421
IPR042277Vacuolar protein sorting-associated protein IST1-likeGENE3D1.20.1260.60coord: 6..177
e-value: 9.2E-46
score: 157.7
IPR005061Vacuolar protein sorting-associated protein Ist1PFAMPF03398Ist1coord: 15..169
e-value: 5.7E-40
score: 136.9
IPR005061Vacuolar protein sorting-associated protein Ist1PANTHERPTHR12161IST1 FAMILY MEMBERcoord: 9..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG17g05670.1Cp4.1LG17g05670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015031 protein transport