Cp4.1LG17g04100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG17g04100 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 994.2 bits (2569), Expect = 8.3e-289
Identity = 503/778 (64.65%), Postives = 600/778 (77.12%), Query Frame = 0

Query: 1   MANLHLCSSGFFF-LILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60
           MA L L S  F F L+LCF     S S    E++IVHV +S KP+LFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120
           L  SP P  LLYSY  A +GFSAR++  Q A LRR P VISVIPD+ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180
            + + GLW+ +NY +DVI+GVLDTGIWPE PSFSD GL P+P+TWKG C+ G  F AS+C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFSGYESAVRRSLQ-GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           NRK+IGARA++ GY +    + +  + + +S RDT+GHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRD 300
           RG A GMAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG  P Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWI+TVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSA 420
           F+G SLY+G+SL DS+L LVY+GDCG R CY G L+SS V GKIV+CDRGGNARV KGSA
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGT 480
           VK AGG GMILANT E+GEEL ADSHL+P TM+G  AG+ ++DYI +  SPTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y +++SG PI DL+T K SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCE-HKL 660
            F+HGAGH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D      CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 TSPGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLE 720
            + G+LNYPSF+VVF S G EVVKY R V NVG  VDAVYEV V+SP  VEI V P+KL 
Sbjct: 661 RTAGDLNYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLA 720

Query: 721 FSAKNRTQSYEITFTKI-----NGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIAS 771
           FS +     YE+TF  +      G      FGSI+W+DG H+V+SPVAV +  G + S
Sbjct: 721 FSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Cp4.1LG17g04100 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 9.9e-226
Identity = 420/765 (54.90%), Postives = 536/765 (70.07%), Query Frame = 0

Query: 8   SSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPT 67
           SS  FFL+LC      S S  +Q T+IVH++KS+ P+ F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGLAD-SQGL 127
           +LLY+YE A +GFS R+T  +A  L   PGVISV+P+   +LHTTRTP FLGL + +  L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGA 187
           + E     DV++GVLDTG+WPE  S+SDEG  P+P++WKG C+ G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R +  GYES +   +  S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYS 367
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDSLGDSKLPLVYAGDCGD----RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKTA 427
           G++L D  LP +YAG+  +      C +GTL   KV GKIV+CDRG NARV KG  VK A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVIGD 487
           GG+GMILANT  NGEEL+AD+HL+P T +GE AG+ ++ Y+ +DP+PTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIIS 547
             P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPFVH 607
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G+P+ D++T K S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCE-HKLTSPG 667
           GAGH+ P  A NPGLIYDL  +DY+ FLC++ Y S QI    + +     C+  K  S  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN---YTCDPSKSYSVA 666

Query: 668 NLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVES-PQGVEITVLPNKLEFSA 727
           +LNYPSFAV  + DG    KYTRTVT+VG      Y VKV S   GV+I+V P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KNRTQSYEITFTKINGIK--NSASFGSIQWSDGSHIVRSPVAVSF 764
            N  +SY +TFT ++  K   S SFGSI+WSDG H+V SPVA+S+
Sbjct: 727 ANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG17g04100 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 3.9e-214
Identity = 387/740 (52.30%), Postives = 504/740 (68.11%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPTKLLYSYELAANGFSARITAAQA 89
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRRIPGVISVIPDRVRQLHTTRTPHFLGLADSQGLWAETNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGARAYFSGYESAVRRSLQGSSDFK 209
             SF D  +  +P+ WKG C++G  F +  CN+K+IGAR++  G++ A         +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWELGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G    P  YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGGGSAP--YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYSGDSLGDSKLPLVY--AGDCGDR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY         
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKTAGGLGMILANTEENGEELLADSHLI 449
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLGMI+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
           P   +G+  G+ L++Y+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPFVHGAGHIDPNKALNPGLIYDLNPQDY 629
           P+AIKSAL+TT+Y LD++  P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSPGNLNYPSFAVVFDSDGDEVVKYTRTV 689
           + FLCS+ Y    I   VK    +  C  K + PG LNYPSF+V+F   G  VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRP--SVNCSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSAKNRTQSYEITFTKINGIK--NSASFG 749
           TNVG    +VY+V V     V I+V P+KL F +    + Y +TF    G+   N A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPVAVSFN 765
           SI WS+  H VRSPVA S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Cp4.1LG17g04100 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 2.2e-209
Identity = 393/776 (50.64%), Postives = 514/776 (66.24%), Query Frame = 0

Query: 11  FFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPTKLL 70
           +FF +L       S S  N  T+IVHV    KP++F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL--ADSQGLWA 130
           ++Y+   +GFSAR+T+  A++L   P VISVIP++VR LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGARA 190
           E+++  D++IGV+DTG+WPERPSF D GL PVP  WKG C     F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           +  GYE A    +  +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYE-ATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG    P  YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVP--YYLDAIAIGAFGAI 305

Query: 311 QHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYSGD 370
             G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 SLGDSKL-PLVYAGDC--GDRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKT 430
            L   ++ PLVY G    GD Y    C  G+LD + V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYI------HSDPSPTATIVF 490
            GGLGMI+AN   +GE L+AD H++P T +G   G+ ++ YI       S   PTATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRR 550
           +GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSK 610
            EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D+SG P+ D ST  
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 QSNPFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVK---DSPYAQL 670
            S+   +G+GH+ P KA++PGL+YD+   DY++FLC+  Y    I    +   D   A+ 
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 665

Query: 671 CEHKLTSPGNLNYPSFAVVFDSDGDEVVK--YTRTVTNVGDEVDAVYEVKVESPQGVEIT 730
             H     GNLNYPSF+VVF   G+  +   + RTVTNVGD  D+VYE+K+  P+G  +T
Sbjct: 666 AGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDS-DSVYEIKIRPPRGTTVT 725

Query: 731 VLPNKLEFSAKNRTQSYEI----TFTKINGIKNSASFGSIQWSDGSHIVRSPVAVS 763
           V P KL F    +  S+ +    T  K++    +   G I WSDG   V SP+ V+
Sbjct: 726 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG17g04100 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 2.1e-207
Identity = 382/784 (48.72%), Postives = 524/784 (66.84%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLI----LCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSI 60
           MAN +     F F+I    L F+    +  +  ++T+++H+ KS  P  +++H  W+SS 
Sbjct: 1   MANKNPLQKPFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSK 60

Query: 61  LESLS------PSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLH 120
           + S++         +  ++LY+Y+ A +G +A++T  +A  L    GV++VIP+   +LH
Sbjct: 61  INSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELH 120

Query: 121 TTRTPHFLGL--ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTC 180
           TTR+P FLGL   +S+ +WAE     DV++GVLDTGIWPE  SF+D G+SPVPATW+G C
Sbjct: 121 TTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGAC 180

Query: 181 QTGVGFSASACNRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSF 240
           +TG  F    CNRKI+GAR ++ GYE+A  + +    ++KS RD DGHGTHTA+T AGS 
Sbjct: 181 ETGKRFLKRNCNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGSP 240

Query: 241 VKNASFFQYARGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGS 300
           VK A+ F +A G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA+ADGV ++S+S+G 
Sbjct: 241 VKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG- 300

Query: 301 SGRPPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFL 360
            G    Y RDS++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F 
Sbjct: 301 -GGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFP 360

Query: 361 ADVILGDGRVFSGVSLYSGDSL--GDSKLPLVYAGDCGD-----RYCYSGTLDSSKVAGK 420
           A V +G  R F GVSLY G ++   + + PLVY G          +C  G LD   VAGK
Sbjct: 361 ATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGK 420

Query: 421 IVVCDRGGNARVAKGSAVKTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKD 480
           IV+CDRG   RV KG  VK AGG+GM+L NT  NGEEL+ADSH++P   +GE  G  +K 
Sbjct: 421 IVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQ 480

Query: 481 YIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWS 540
           Y  +    TA++   GT IG   P+P VAAFSSRGPN+ + EILKPD++APGVNILA W+
Sbjct: 481 YAMTSKKATASLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWT 540

Query: 541 GYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSL 600
           G  +P+SLS DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  
Sbjct: 541 GDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVH 600

Query: 601 DSSGRPITDLSTSKQSNPFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIA 660
           D+  +P+TD S +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ 
Sbjct: 601 DNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLK 660

Query: 661 VFVKDSPYAQLCEHKLT-SPGNLNYPSFAVVF-DSDGDEVVKYTRTVTNVGDEVDAVYEV 720
           VF K S   + C+H L  +PGNLNYP+ + +F ++   + +   RTVTNVG  + + Y+V
Sbjct: 661 VFTKHS--NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKV 720

Query: 721 KVESPQGVEITVLPNKLEFSAKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPV 764
            V   +G  +TV P  L F++K++  SY +TF     +K    FG + W   +H VRSPV
Sbjct: 721 SVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPV 776

BLAST of Cp4.1LG17g04100 vs. NCBI nr
Match: XP_023514101.1 (subtilisin-like protease SBT1.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1532 bits (3966), Expect = 0.0
Identity = 771/771 (100.00%), Postives = 771/771 (100.00%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. NCBI nr
Match: XP_022964269.1 (subtilisin-like protease SBT1.4 [Cucurbita moschata])

HSP 1 Score: 1528 bits (3956), Expect = 0.0
Identity = 769/771 (99.74%), Postives = 770/771 (99.87%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           K+RTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. NCBI nr
Match: KAG6593557.1 (Subtilisin-like protease 1.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1523 bits (3942), Expect = 0.0
Identity = 767/771 (99.48%), Postives = 767/771 (99.48%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANL LCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL
Sbjct: 1   MANLRLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           KN TQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KNLTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. NCBI nr
Match: XP_023000019.1 (subtilisin-like protease SBT1.4 [Cucurbita maxima])

HSP 1 Score: 1514 bits (3920), Expect = 0.0
Identity = 760/771 (98.57%), Postives = 768/771 (99.61%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANLHLCSS FFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSIL+SL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLY+YELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMA+RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYA+LC+HKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESP+GVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. NCBI nr
Match: XP_023548434.1 (subtilisin-like protease SBT1.4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1377 bits (3564), Expect = 0.0
Identity = 681/771 (88.33%), Postives = 726/771 (94.16%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MAN H CSS FFF  LCF+PVIFSRS   QETFIVHVS+SEKP+LFSSH+ WHSSIL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSSDKQETFIVHVSRSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPS  P KLLY+YE AANGF+ARIT AQAAELRR+PGV+SVIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAANGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+ G GFSA+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F GYES +RR L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIRRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           +AGGLG+ILANTEENGEELLADSHLIPGTMIGEIAGN L+DYIHSDP+P+ATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
            DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 SDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SGRPI DLSTS  SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHG GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDS Y+ LCEHKL++P
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVF S+G+EVVKYTRTVTNVGD+ DAVYEVKVE+PQG+EI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDKADAVYEVKVEAPQGIEISVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           K  TQSYEITFTKI+G KNSASFGSIQWSDGSHIVRSP+AVSFN+  IASM
Sbjct: 721 KKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Cp4.1LG17g04100 vs. ExPASy TrEMBL
Match: A0A6J1HMN7 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111464338 PE=3 SV=1)

HSP 1 Score: 1528 bits (3956), Expect = 0.0
Identity = 769/771 (99.74%), Postives = 770/771 (99.87%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL
Sbjct: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           K+RTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KDRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. ExPASy TrEMBL
Match: A0A6J1KIR1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111494328 PE=3 SV=1)

HSP 1 Score: 1514 bits (3920), Expect = 0.0
Identity = 760/771 (98.57%), Postives = 768/771 (99.61%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MANLHLCSS FFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSIL+SL
Sbjct: 1   MANLHLCSSVFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPSPHPTKLLY+YELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL
Sbjct: 61  SPSPHPTKLLYNYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN
Sbjct: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMA+RARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI
Sbjct: 241 EARGMANRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVYAGDCG+RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGS VK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYAGDCGNRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSTVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI
Sbjct: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPN+RTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNFRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYA+LC+HKLTSP
Sbjct: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAELCKHKLTSP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESP+GVEITVLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPEGVEITVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM
Sbjct: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771

BLAST of Cp4.1LG17g04100 vs. ExPASy TrEMBL
Match: A0A6J1JUV1 (subtilisin-like protease SBT1.4 OS=Cucurbita maxima OX=3661 GN=LOC111488562 PE=3 SV=1)

HSP 1 Score: 1369 bits (3544), Expect = 0.0
Identity = 679/771 (88.07%), Postives = 721/771 (93.51%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESL 60
           MAN H CSS FFF  LCF+PVIFSRS   QETFIVHVSKSEKP+LFSSH+ WHSSIL+SL
Sbjct: 1   MANFHRCSSVFFFFFLCFLPVIFSRSPDKQETFIVHVSKSEKPSLFSSHYRWHSSILDSL 60

Query: 61  SPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL 120
           SPS  P KLLY+YE A NGF+ARITA QAAELRR+PGV+ VIPDR+RQLHTTRTPHFLGL
Sbjct: 61  SPSSRPRKLLYNYERAVNGFAARITADQAAELRRVPGVVLVIPDRMRQLHTTRTPHFLGL 120

Query: 121 ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACN 180
           AD+ GLW +TNYADDVIIGVLDTGIWPE PSFSDEGLSPVPA+WKGTC+ G GFSA+ CN
Sbjct: 121 ADNLGLWPDTNYADDVIIGVLDTGIWPEHPSFSDEGLSPVPASWKGTCEAGEGFSATTCN 180

Query: 181 RKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARG 240
           RKIIGARA+F GYES + R L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYARG
Sbjct: 181 RKIIGARAFFHGYESNIGRRLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYARG 240

Query: 241 EARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSI 300
           EARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDSI
Sbjct: 241 EARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDSI 300

Query: 301 AIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFS 360
           AIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVFS
Sbjct: 301 AIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVFS 360

Query: 361 GVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVK 420
           GVSLYSGD LGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAVK
Sbjct: 361 GVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAVK 420

Query: 421 TAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVI 480
           +AGGLG+ILANTEENGEELLADSHLIPGTMIGEIAGN L+DYIHSDP+P+ATIVFRGTVI
Sbjct: 421 SAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTVI 480

Query: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNI 540
           GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFNI
Sbjct: 481 GDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFNI 540

Query: 541 ISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPF 600
           ISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SG PI DLSTS  SNPF
Sbjct: 541 ISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGSPIKDLSTSNDSNPF 600

Query: 601 VHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSP 660
           VHG GHIDPNKALNPGLIYDLNP DYVSFLCSIGYDSRQIAVFVKDS Y+ LCEHKL++P
Sbjct: 601 VHGGGHIDPNKALNPGLIYDLNPHDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSNP 660

Query: 661 GNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSA 720
           GNLNYPSFAVVF S+G+EVVKYTRTVTNVGDE DAVYEVKVE+PQGVEI+VLPNKLEFSA
Sbjct: 661 GNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFSA 720

Query: 721 KNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           K  T+SYEITFTKI+G KNSASFGSIQWSDGSHIVRSP+AVSFN+  IASM
Sbjct: 721 KKTTESYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 771

BLAST of Cp4.1LG17g04100 vs. ExPASy TrEMBL
Match: A0A6J1GQK5 (subtilisin-like protease SBT1.4 OS=Cucurbita moschata OX=3662 GN=LOC111456188 PE=3 SV=1)

HSP 1 Score: 1368 bits (3541), Expect = 0.0
Identity = 680/772 (88.08%), Postives = 723/772 (93.65%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLI-LCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60
           MAN H CSS FFF   LCF+PVIFSRS   QETFIVHVSK EKP+LFSSH+ WHSSIL+S
Sbjct: 1   MANFHRCSSVFFFFFFLCFLPVIFSRSPDKQETFIVHVSKLEKPSLFSSHYRWHSSILDS 60

Query: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120
           LSPS  P KLLY+YE A NGF+ARIT AQAAELRR+PGV+SVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSSRPRKLLYNYERAVNGFAARITDAQAAELRRVPGVVSVIPDRMRQLHTTRTPHFLG 120

Query: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180
           LAD+ GLW +TNYADDVIIGVLDTGIWPERPSFSDEGLSPVPA+WKGTC+ G GFSA+ C
Sbjct: 121 LADNLGLWPDTNYADDVIIGVLDTGIWPERPSFSDEGLSPVPASWKGTCEAGEGFSATTC 180

Query: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYES + R L G SDFKSARD+DGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFHGYESNIGRPLHGLSDFKSARDSDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAIADGVHIISLSVGSSGR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAIADGVHIISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGDGRVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWILTVGASTIDREFLADVILGDGRVF 360

Query: 361 SGVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGD LGDSKLPLVY GDCG+RYCYSG+LD SKVAGKIVVCDRGGNARVAKGSAV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGNRYCYSGSLDPSKVAGKIVVCDRGGNARVAKGSAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480
           K+AGGLG+ILANTEENGEELLADSHLIPGTMIGEIAGN L+DYIHSDP+P+ATIVFRGT+
Sbjct: 421 KSAGGLGVILANTEENGEELLADSHLIPGTMIGEIAGNKLRDYIHSDPNPSATIVFRGTI 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           I DSPP+PRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGY+SP+SLSID RRVEFN
Sbjct: 481 ISDSPPSPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYNSPSSLSIDTRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600
           IISGTSMSCPHVSGVAALLRKAFPTW+PAAIKSAL+TTSYSLD+SGRPI DLSTS  SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWTPAAIKSALITTSYSLDTSGRPIKDLSTSNDSNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660
           FVHG GHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDS Y+ LCEHKL++
Sbjct: 601 FVHGGGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSSYSNLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720
           PGNLNYPSFAVVF S+G+EVVKYTRTVTNVGDE DAVYEVKVE+PQGVEI+VLPNKLEFS
Sbjct: 661 PGNLNYPSFAVVFTSNGEEVVKYTRTVTNVGDEADAVYEVKVEAPQGVEISVLPNKLEFS 720

Query: 721 AKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
           AK  TQSYEITFTKI+G KNSASFGSIQWSDGSHIVRSP+AVSFN+  IASM
Sbjct: 721 AKKTTQSYEITFTKISGFKNSASFGSIQWSDGSHIVRSPIAVSFNTASIASM 772

BLAST of Cp4.1LG17g04100 vs. ExPASy TrEMBL
Match: A0A1S3CGN8 (LOW QUALITY PROTEIN: subtilisin-like protease SBT1.4 OS=Cucumis melo OX=3656 GN=LOC103500685 PE=3 SV=1)

HSP 1 Score: 1353 bits (3502), Expect = 0.0
Identity = 673/772 (87.18%), Postives = 722/772 (93.52%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLILCFV-PVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60
           MAN HL SS FFF  + FV P++FSRSL+ QET+IVHVSKSEKP+LFSSHHHWHSSILES
Sbjct: 1   MANFHLPSSSFFFFFVLFVFPLVFSRSLETQETYIVHVSKSEKPSLFSSHHHWHSSILES 60

Query: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120
           LSPSPHPTKLLY+YE AANGFSARIT AQA ELRR+PG+ISVIPDR+RQLHTTRTPHFLG
Sbjct: 61  LSPSPHPTKLLYNYERAANGFSARITTAQAEELRRVPGIISVIPDRIRQLHTTRTPHFLG 120

Query: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180
           LAD+ GLWA+TNYADDVIIGVLDTGIWPERPSFSDEGL+PVPA WKGTC TG G SA AC
Sbjct: 121 LADNLGLWADTNYADDVIIGVLDTGIWPERPSFSDEGLAPVPARWKGTCDTGEGSSALAC 180

Query: 181 NRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240
           NRKIIGARA+F GYES +R SL+GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR
Sbjct: 181 NRKIIGARAFFXGYESNLRGSLKGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYAR 240

Query: 241 GEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDS 300
           GEARGMASRARIAAYKICWE GCYDSDILAAMDQAI+DGV +ISLSVGSSGR PAYYRDS
Sbjct: 241 GEARGMASRARIAAYKICWEFGCYDSDILAAMDQAISDGVDVISLSVGSSGRAPAYYRDS 300

Query: 301 IAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVF 360
           IAIGAFGAMQHG+VVSCSAGNSGPGPYTAVNIAPWI+TVGASTIDREFLADVILGD RVF
Sbjct: 301 IAIGAFGAMQHGIVVSCSAGNSGPGPYTAVNIAPWIVTVGASTIDREFLADVILGDNRVF 360

Query: 361 SGVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAV 420
           SGVSLYSGD LGDSKLPLVY GDCG RYCYSG+LDSSKVAG+IVVCDRGGNARVAKG AV
Sbjct: 361 SGVSLYSGDPLGDSKLPLVYGGDCGSRYCYSGSLDSSKVAGRIVVCDRGGNARVAKGGAV 420

Query: 421 KTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTV 480
           K+AGGLGM+LANTEENGEELLADSHLIPGTM+GEIAGN L+DYIH+DP+PTATIVFRGTV
Sbjct: 421 KSAGGLGMVLANTEENGEELLADSHLIPGTMVGEIAGNKLRDYIHTDPNPTATIVFRGTV 480

Query: 481 IGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFN 540
           IGDSPPAP+VA+FSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPT L+IDPRRVEFN
Sbjct: 481 IGDSPPAPKVASFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTGLNIDPRRVEFN 540

Query: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNP 600
           IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSAL+TTSYSLDSSG PI DL+TS++SNP
Sbjct: 541 IISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALITTSYSLDSSGSPIKDLATSEESNP 600

Query: 601 FVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTS 660
           FVHGAGHIDPN+ALNPGLIYDL PQDYVSFLCSIGYDS+QIAVFVK S Y+QLCEHKL++
Sbjct: 601 FVHGAGHIDPNQALNPGLIYDLTPQDYVSFLCSIGYDSKQIAVFVKGSSYSQLCEHKLSN 660

Query: 661 PGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLEFS 720
           PGNLNYPSF+VVFD DG EVVKYTRTVTNVGDE + VY VKVE+PQGVEI+V+PNKLEF+
Sbjct: 661 PGNLNYPSFSVVFD-DG-EVVKYTRTVTNVGDETEVVYGVKVEAPQGVEISVVPNKLEFN 720

Query: 721 AKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIASM 771
            +  T SYEITFTKING K SASFGSIQWSDG H VRSP+AVSF +G IASM
Sbjct: 721 KEKTTLSYEITFTKINGFKESASFGSIQWSDGIHNVRSPIAVSFKTGSIASM 770

BLAST of Cp4.1LG17g04100 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 994.2 bits (2569), Expect = 5.9e-290
Identity = 503/778 (64.65%), Postives = 600/778 (77.12%), Query Frame = 0

Query: 1   MANLHLCSSGFFF-LILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILES 60
           MA L L S  F F L+LCF     S S    E++IVHV +S KP+LFSSH++WH S+L S
Sbjct: 1   MAKLSLSSIFFVFPLLLCFFSP-SSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRS 60

Query: 61  LSPSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLG 120
           L  SP P  LLYSY  A +GFSAR++  Q A LRR P VISVIPD+ R++HTT TP FLG
Sbjct: 61  LPSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLG 120

Query: 121 LADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASAC 180
            + + GLW+ +NY +DVI+GVLDTGIWPE PSFSD GL P+P+TWKG C+ G  F AS+C
Sbjct: 121 FSQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSC 180

Query: 181 NRKIIGARAYFSGYESAVRRSLQ-GSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYA 240
           NRK+IGARA++ GY +    + +  + + +S RDT+GHGTHTASTAAGS V NAS +QYA
Sbjct: 181 NRKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYA 240

Query: 241 RGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRD 300
           RG A GMAS+ARIAAYKICW  GCYDSDILAAMDQA+ADGVH+ISLSVG+SG  P Y+ D
Sbjct: 241 RGTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTD 300

Query: 301 SIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRV 360
           SIAIGAFGA +HG+VVSCSAGNSGP P TA NIAPWI+TVGAST+DREF A+ I GDG+V
Sbjct: 301 SIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKV 360

Query: 361 FSGVSLYSGDSLGDSKLPLVYAGDCGDRYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSA 420
           F+G SLY+G+SL DS+L LVY+GDCG R CY G L+SS V GKIV+CDRGGNARV KGSA
Sbjct: 361 FTGTSLYAGESLPDSQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSA 420

Query: 421 VKTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGT 480
           VK AGG GMILANT E+GEEL ADSHL+P TM+G  AG+ ++DYI +  SPTA I F GT
Sbjct: 421 VKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGT 480

Query: 481 VIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEF 540
           +IG SPP+PRVAAFSSRGPN+ T  ILKPDVIAPGVNILAGW+G   PT L IDPRRV+F
Sbjct: 481 LIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQF 540

Query: 541 NIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSN 600
           NIISGTSMSCPHVSG+AALLRKA P WSPAAIKSALVTT+Y +++SG PI DL+T K SN
Sbjct: 541 NIISGTSMSCPHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSN 600

Query: 601 PFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCE-HKL 660
            F+HGAGH+DPNKALNPGL+YD+  ++YV+FLC++GY+   I VF++D      CE  KL
Sbjct: 601 SFIHGAGHVDPNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKL 660

Query: 661 TSPGNLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVESPQGVEITVLPNKLE 720
            + G+LNYPSF+VVF S G EVVKY R V NVG  VDAVYEV V+SP  VEI V P+KL 
Sbjct: 661 RTAGDLNYPSFSVVFASTG-EVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLA 720

Query: 721 FSAKNRTQSYEITFTKI-----NGIKNSASFGSIQWSDGSHIVRSPVAVSFNSGYIAS 771
           FS +     YE+TF  +      G      FGSI+W+DG H+V+SPVAV +  G + S
Sbjct: 721 FSKEKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQWGQGSVQS 776

BLAST of Cp4.1LG17g04100 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 784.6 bits (2025), Expect = 7.1e-227
Identity = 420/765 (54.90%), Postives = 536/765 (70.07%), Query Frame = 0

Query: 8   SSGFFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPT 67
           SS  FFL+LC      S S  +Q T+IVH++KS+ P+ F  H +W+ S L S+S S    
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDS---A 66

Query: 68  KLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGLAD-SQGL 127
           +LLY+YE A +GFS R+T  +A  L   PGVISV+P+   +LHTTRTP FLGL + +  L
Sbjct: 67  ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 126

Query: 128 WAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGA 187
           + E     DV++GVLDTG+WPE  S+SDEG  P+P++WKG C+ G  F+AS CNRK+IGA
Sbjct: 127 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 186

Query: 188 RAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMA 247
           R +  GYES +   +  S + +S RD DGHGTHT+STAAGS V+ AS   YA G ARGMA
Sbjct: 187 RFFARGYESTM-GPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMA 246

Query: 248 SRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFG 307
            RAR+A YK+CW  GC+ SDILAA+D+AIAD V+++S+S+G  G    YYRD +AIGAF 
Sbjct: 247 PRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLG--GGMSDYYRDGVAIGAFA 306

Query: 308 AMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYS 367
           AM+ G++VSCSAGN+GP   +  N+APWI TVGA T+DR+F A  ILG+G+ F+GVSL+ 
Sbjct: 307 AMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFK 366

Query: 368 GDSLGDSKLPLVYAGDCGD----RYCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKTA 427
           G++L D  LP +YAG+  +      C +GTL   KV GKIV+CDRG NARV KG  VK A
Sbjct: 367 GEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAA 426

Query: 428 GGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVIGD 487
           GG+GMILANT  NGEEL+AD+HL+P T +GE AG+ ++ Y+ +DP+PTA+I   GTV+G 
Sbjct: 427 GGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG- 486

Query: 488 SPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIIS 547
             P+P VAAFSSRGPN  T  ILKPD+IAPGVNILA W+G + PT L+ D RRVEFNIIS
Sbjct: 487 VKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIIS 546

Query: 548 GTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPFVH 607
           GTSMSCPHVSG+AALL+   P WSPAAI+SAL+TT+Y     G+P+ D++T K S PF H
Sbjct: 547 GTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDH 606

Query: 608 GAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVKDSPYAQLCE-HKLTSPG 667
           GAGH+ P  A NPGLIYDL  +DY+ FLC++ Y S QI    + +     C+  K  S  
Sbjct: 607 GAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN---YTCDPSKSYSVA 666

Query: 668 NLNYPSFAVVFDSDGDEVVKYTRTVTNVGDEVDAVYEVKVES-PQGVEITVLPNKLEFSA 727
           +LNYPSFAV  + DG    KYTRTVT+VG      Y VKV S   GV+I+V P  L F  
Sbjct: 667 DLNYPSFAV--NVDGVGAYKYTRTVTSVGGA--GTYSVKVTSETTGVKISVEPAVLNFKE 726

Query: 728 KNRTQSYEITFTKINGIK--NSASFGSIQWSDGSHIVRSPVAVSF 764
            N  +SY +TFT ++  K   S SFGSI+WSDG H+V SPVA+S+
Sbjct: 727 ANEKKSYTVTFT-VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISW 756

BLAST of Cp4.1LG17g04100 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 746.1 bits (1925), Expect = 2.8e-215
Identity = 387/740 (52.30%), Postives = 504/740 (68.11%), Query Frame = 0

Query: 30  QETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPTKLLYSYELAANGFSARITAAQA 89
           ++T+I+ V+ S+KP  F +HH W++S L S S       LLY+Y  + +GFSA + + +A
Sbjct: 27  KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES------SLLYTYTTSFHGFSAYLDSTEA 86

Query: 90  -AELRRIPGVISVIPDRVRQLHTTRTPHFLGLADSQGLWAETNYADDVIIGVLDTGIWPE 149
            + L     ++ +  D +  LHTTRTP FLGL    G+    + ++ VIIGVLDTG+WPE
Sbjct: 87  DSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPE 146

Query: 150 RPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGARAYFSGYESAVRRSLQGSSDFK 209
             SF D  +  +P+ WKG C++G  F +  CN+K+IGAR++  G++ A         +  
Sbjct: 147 SRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESV 206

Query: 210 SARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASRARIAAYKICWELGCYDSDIL 269
           S RD DGHGTHT++TAAGS V+NASF  YA G ARGMA+RAR+A YK+CW  GC+ SDIL
Sbjct: 207 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDIL 266

Query: 270 AAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTA 329
           AAMD+AI DGV ++SLS+G    P  YYRD+IAIGAF AM+ GV VSCSAGNSGP   + 
Sbjct: 267 AAMDRAILDGVDVLSLSLGGGSAP--YYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 326

Query: 330 VNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYSGDSLGDSKLPLVY--AGDCGDR 389
            N+APW++TVGA T+DR+F A   LG+G+  +GVSLYSG  +G   L LVY         
Sbjct: 327 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSN 386

Query: 390 YCYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKTAGGLGMILANTEENGEELLADSHLI 449
            C  G+LDSS V GKIVVCDRG NARV KG+ V+ AGGLGMI+ANT  +GEEL+ADSHL+
Sbjct: 387 LCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLL 446

Query: 450 PGTMIGEIAGNTLKDYIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILK 509
           P   +G+  G+ L++Y+ SD  PTA +VF+GTV+ D  P+P VAAFSSRGPN  T EILK
Sbjct: 447 PAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVL-DVKPSPVVAAFSSRGPNTVTPEILK 506

Query: 510 PDVIAPGVNILAGWSGYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWS 569
           PDVI PGVNILAGWS    PT L  D RR +FNI+SGTSMSCPH+SG+A LL+ A P WS
Sbjct: 507 PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWS 566

Query: 570 PAAIKSALVTTSYSLDSSGRPITDLSTSKQSNPFVHGAGHIDPNKALNPGLIYDLNPQDY 629
           P+AIKSAL+TT+Y LD++  P+ D + +  SNP+ HG+GH+DP KAL+PGL+YD++ ++Y
Sbjct: 567 PSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEY 626

Query: 630 VSFLCSIGYDSRQIAVFVKDSPYAQLCEHKLTSPGNLNYPSFAVVFDSDGDEVVKYTRTV 689
           + FLCS+ Y    I   VK    +  C  K + PG LNYPSF+V+F   G  VV+YTR V
Sbjct: 627 IRFLCSLDYTVDHIVAIVKRP--SVNCSKKFSDPGQLNYPSFSVLF--GGKRVVRYTREV 686

Query: 690 TNVGDEVDAVYEVKVESPQGVEITVLPNKLEFSAKNRTQSYEITFTKINGIK--NSASFG 749
           TNVG    +VY+V V     V I+V P+KL F +    + Y +TF    G+   N A FG
Sbjct: 687 TNVG-AASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 746

Query: 750 SIQWSDGSHIVRSPVAVSFN 765
           SI WS+  H VRSPVA S+N
Sbjct: 747 SITWSNPQHEVRSPVAFSWN 752

BLAST of Cp4.1LG17g04100 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 730.3 bits (1884), Expect = 1.6e-210
Identity = 393/776 (50.64%), Postives = 514/776 (66.24%), Query Frame = 0

Query: 11  FFFLILCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSILESLSPSPHPTKLL 70
           +FF +L       S S  N  T+IVHV    KP++F +H HW++S L SL+ S  P  ++
Sbjct: 6   YFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSS--PPSII 65

Query: 71  YSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLHTTRTPHFLGL--ADSQGLWA 130
           ++Y+   +GFSAR+T+  A++L   P VISVIP++VR LHTTR+P FLGL   D  GL  
Sbjct: 66  HTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLE 125

Query: 131 ETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTCQTGVGFSASACNRKIIGARA 190
           E+++  D++IGV+DTG+WPERPSF D GL PVP  WKG C     F  SACNRK++GAR 
Sbjct: 126 ESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARF 185

Query: 191 YFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSFVKNASFFQYARGEARGMASR 250
           +  GYE A    +  +++F+S RD+DGHGTHTAS +AG +V  AS   YA G A GMA +
Sbjct: 186 FCGGYE-ATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPK 245

Query: 251 ARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGSSGRPPAYYRDSIAIGAFGAM 310
           AR+AAYK+CW  GCYDSDILAA D A+ADGV +ISLSVG    P  YY D+IAIGAFGA+
Sbjct: 246 ARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVP--YYLDAIAIGAFGAI 305

Query: 311 QHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFLADVILGDGRVFSGVSLYSGD 370
             G+ VS SAGN GPG  T  N+APW+ TVGA TIDR+F A+V LG+G++ SGVS+Y G 
Sbjct: 306 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 365

Query: 371 SLGDSKL-PLVYAGDC--GDRY----CYSGTLDSSKVAGKIVVCDRGGNARVAKGSAVKT 430
            L   ++ PLVY G    GD Y    C  G+LD + V GKIV+CDRG N+R  KG  V+ 
Sbjct: 366 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 425

Query: 431 AGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKDYI------HSDPSPTATIVF 490
            GGLGMI+AN   +GE L+AD H++P T +G   G+ ++ YI       S   PTATIVF
Sbjct: 426 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 485

Query: 491 RGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWSGYSSPTSLSIDPRR 550
           +GT +G   PAP VA+FS+RGPN  T EILKPDVIAPG+NILA W     P+ ++ D RR
Sbjct: 486 KGTRLG-IRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRR 545

Query: 551 VEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSLDSSGRPITDLSTSK 610
            EFNI+SGTSM+CPHVSG+AALL+ A P WSPAAI+SAL+TT+Y++D+SG P+ D ST  
Sbjct: 546 TEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN 605

Query: 611 QSNPFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIAVFVK---DSPYAQL 670
            S+   +G+GH+ P KA++PGL+YD+   DY++FLC+  Y    I    +   D   A+ 
Sbjct: 606 TSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARR 665

Query: 671 CEHKLTSPGNLNYPSFAVVFDSDGDEVVK--YTRTVTNVGDEVDAVYEVKVESPQGVEIT 730
             H     GNLNYPSF+VVF   G+  +   + RTVTNVGD  D+VYE+K+  P+G  +T
Sbjct: 666 AGH----VGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDS-DSVYEIKIRPPRGTTVT 725

Query: 731 VLPNKLEFSAKNRTQSYEI----TFTKINGIKNSASFGSIQWSDGSHIVRSPVAVS 763
           V P KL F    +  S+ +    T  K++    +   G I WSDG   V SP+ V+
Sbjct: 726 VEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770

BLAST of Cp4.1LG17g04100 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 723.8 bits (1867), Expect = 1.5e-208
Identity = 382/784 (48.72%), Postives = 524/784 (66.84%), Query Frame = 0

Query: 1   MANLHLCSSGFFFLI----LCFVPVIFSRSLQNQETFIVHVSKSEKPALFSSHHHWHSSI 60
           MAN +     F F+I    L F+    +  +  ++T+++H+ KS  P  +++H  W+SS 
Sbjct: 1   MANKNPLQKPFLFIILSINLIFLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSK 60

Query: 61  LESLS------PSPHPTKLLYSYELAANGFSARITAAQAAELRRIPGVISVIPDRVRQLH 120
           + S++         +  ++LY+Y+ A +G +A++T  +A  L    GV++VIP+   +LH
Sbjct: 61  INSVTQHKSQEEEGNNNRILYTYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELH 120

Query: 121 TTRTPHFLGL--ADSQGLWAETNYADDVIIGVLDTGIWPERPSFSDEGLSPVPATWKGTC 180
           TTR+P FLGL   +S+ +WAE     DV++GVLDTGIWPE  SF+D G+SPVPATW+G C
Sbjct: 121 TTRSPTFLGLERQESERVWAERVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGAC 180

Query: 181 QTGVGFSASACNRKIIGARAYFSGYESAVRRSLQGSSDFKSARDTDGHGTHTASTAAGSF 240
           +TG  F    CNRKI+GAR ++ GYE+A  + +    ++KS RD DGHGTHTA+T AGS 
Sbjct: 181 ETGKRFLKRNCNRKIVGARVFYRGYEAATGK-IDEELEYKSPRDRDGHGTHTAATVAGSP 240

Query: 241 VKNASFFQYARGEARGMASRARIAAYKICWELGCYDSDILAAMDQAIADGVHIISLSVGS 300
           VK A+ F +A G ARGMA +AR+AAYK+CW  GC+ SDIL+A+DQA+ADGV ++S+S+G 
Sbjct: 241 VKGANLFGFAYGTARGMAQKARVAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLG- 300

Query: 301 SGRPPAYYRDSIAIGAFGAMQHGVVVSCSAGNSGPGPYTAVNIAPWIITVGASTIDREFL 360
            G    Y RDS++I  FGAM+ GV VSCSAGN GP P +  N++PWI TVGAST+DR+F 
Sbjct: 301 -GGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFP 360

Query: 361 ADVILGDGRVFSGVSLYSGDSL--GDSKLPLVYAGDCGD-----RYCYSGTLDSSKVAGK 420
           A V +G  R F GVSLY G ++   + + PLVY G          +C  G LD   VAGK
Sbjct: 361 ATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGK 420

Query: 421 IVVCDRGGNARVAKGSAVKTAGGLGMILANTEENGEELLADSHLIPGTMIGEIAGNTLKD 480
           IV+CDRG   RV KG  VK AGG+GM+L NT  NGEEL+ADSH++P   +GE  G  +K 
Sbjct: 421 IVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQ 480

Query: 481 YIHSDPSPTATIVFRGTVIGDSPPAPRVAAFSSRGPNYRTAEILKPDVIAPGVNILAGWS 540
           Y  +    TA++   GT IG   P+P VAAFSSRGPN+ + EILKPD++APGVNILA W+
Sbjct: 481 YAMTSKKATASLEILGTRIG-IKPSPVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWT 540

Query: 541 GYSSPTSLSIDPRRVEFNIISGTSMSCPHVSGVAALLRKAFPTWSPAAIKSALVTTSYSL 600
           G  +P+SLS DPRRV+FNI+SGTSMSCPHVSGVAAL++   P WSPAAIKSAL+TT+Y  
Sbjct: 541 GDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVH 600

Query: 601 DSSGRPITDLSTSKQSNPFVHGAGHIDPNKALNPGLIYDLNPQDYVSFLCSIGYDSRQIA 660
           D+  +P+TD S +  S+P+ HGAGHIDP +A +PGL+YD+ PQ+Y  FLC+      Q+ 
Sbjct: 601 DNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLK 660

Query: 661 VFVKDSPYAQLCEHKLT-SPGNLNYPSFAVVF-DSDGDEVVKYTRTVTNVGDEVDAVYEV 720
           VF K S   + C+H L  +PGNLNYP+ + +F ++   + +   RTVTNVG  + + Y+V
Sbjct: 661 VFTKHS--NRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRTVTNVGPHISS-YKV 720

Query: 721 KVESPQGVEITVLPNKLEFSAKNRTQSYEITFTKINGIKNSASFGSIQWSDGSHIVRSPV 764
            V   +G  +TV P  L F++K++  SY +TF     +K    FG + W   +H VRSPV
Sbjct: 721 SVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKR-PEFGGLVWKSTTHKVRSPV 776