Cp4.1LG17g04020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG17g04020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionLong-chain-fatty-acid CoA ligase, putative
LocationCp4.1LG17: 2811681 .. 2814574 (-)
RNA-Seq ExpressionCp4.1LG17g04020
SyntenyCp4.1LG17g04020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCGGTTCCATTTAGGTTAGGAGTAAAAAATTTGTTCGTCCATTTTTCCGTAACCCATCTGTTCTTGGATATACCTCTGTTTTACTTCAATATTCTTATGCTCTGTGGCATTTGAATGTTGATGATGGGCATGGCGGTTCCTTTTACTTGCAATCAGCACTCGTGGATTTCTACCATTTCTGGCCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTAATTTTTGAAATCCGTTTTGAATGTCATTATGTTATGATTTAGAGAGAAATTGAATGAATTATAATGTAGAGAGAGAGGTTGGAGTTAAGGAGGTATTGTCCAATGCTAGAAAGTTGTTTGGTGTTAGGTATTGGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATCGCGATCGGGTAGCATTACTGGATCCATACCATAATCCTGCTTCCAAAATTACTTATTAACAGGTATCCATACCATAATCTTGTTAGTATTCCTCGTTTGCTTCTTCAGCATAGTCAGTGATTATGTTTTTGCTTCATTTTAGCAACGAAACACCATCTTGTTTCTTATATTACTTGCTTTTCTTTTCTTTTTTTCTTCGTAAATTTAAATTTGCAGCTAGAACAAAGTATCTTGAATTCCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATCGCACTCTTTTCTGATAATTTATGCCGATGGCTTGTTGCCGATCAAATATTGTATTTCATTTTGTTATATTTTAATAATTTGAGTTCAGGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACTCTGAAAGGTCTCTTAAATTGCTTCATATTTACTGTACGTTGCAGAGAATCAAAGGTTTTAATATAAACTATGTTTTGGTTTTAAATTTAAACTTTCGCATTAGTTGTAGACAACCCTGAGGCTTATGGAGCTCTCGAATAGTCTTTCCTTAATCGAGGCCCAACTCTTTTCTTAGAGCCCTCGAACAAAGTACACACTTTGTTCGACATTTTAGTCACTTTTGACTACACCTTCGAGGCTCACAATTCTTTGTTCGATATTTGAGGATTCTATTTCACGGCTAAGTTTAGGGTCATGGCTCTGATACCATGTTAGGAATCACATATCTGCACAATGGTATGATATTGTCCTCTTTGAGTATAAGCTCTCATGGCTTTGGTTTGGGATTCCCCAAAAGGTCTCGTACCAATGGAGATACCCATGATCTTCCACTAAATTAACCAACGTGGGACTCACACCCAATAATCTTCGACATTTGTTTCATTACTCTCTTTATTTCACCAAAGTATGATGTGTTCTAAGGCTGCTTGGTATGTCAAACATTTTCCTGATAAAGAATTTATGGGATATTATACCTGCTAAAGTTGGGGATAAATTTCTAAGCATGCTTCCACCTTGGCATGCGTACGAAGGGCTTGTGAATATTTCATATTTACACATGGAGTGGAGCAAGCTTACATAACAACGAGAAAGTTAAAGGTATTTCACTTGCACCAAATCTGGGAAATTAGAATTTTTTTAAACTCTTCTGTATTTGAGTTGTCAGGATGATCTTCGTGATTATCAACCAGACTACTTGGTTTCTGTTCCATTGGTGTGATTATAGTTCCCAACAAGGGACAATTGTTGTCTGCTATATTCGATCTTAGCAATCAAACCTTGACAAACCTGATTTATAGTGAACTCATAAAGTGGTTAGACTTTTCTGATGTTCCTATGTTCATCATTAGCCATTTACTGTTCGTTCTTAGTCTTTTGTGAAGAAGGAAGGGTGGTTGATAGCAATATAATATGTTGGTTTCATGCAGGACTTCAGAGTGTTCATTTCAAATTGGACCAATCCTCATTGCCAATGAACCATTCACGGTATGAAATTCGTGAATTTAATATAAAAAGATACTTCCAATTTGCAAATCTTTTAATCTACTTTTGAACACTTCGAATTGTAGATGGATAATGGGTTACTAAGCCCTACATTGAAAGTTTGAAGACACAAGGTTACTTCTTATTACAAGAAGGAAATAGAGAACTTATTCAAGGCAATATAATGTGGTAAGGCAATATAATGTGGTTAATTAGTATCTGGACTTGGACAAACCTTTAGCACAAGCTATATATATAAATAACTTTATAATATGCTACATGTGATACAAGCCTTTATATGATATTCTAGGATTAGTCCATGAATGTAAGTACCAAAGTACTATCCTAAGGCCATGAACTTAGTTAAGTAAGTCCACCACACACCCCATTCCTTAAAAGTTACCGTGACCTTGGTAGGTGCTATTCCAACCCAAATCTCATGATTTCATGTTTTTCTCATCTAGATAAGATGTCATGTGCCGTAGGATGTTATGTTCATGCTTTCATGTTTTCTTCGACTAGATTTTTACTGTAATGTTTTGGCCTGATTAATGCCCGATTTATGGACTTGAGGAATTGGGTATTCAAAGATTTTCACCATACACTTTCACTATAAAATTTAATTAGATGAAATTGAGTTGAGATAAAGCCTCAATTTGAGTATATTGTTACAAATTAGGAAGAGAGGTCCCACAAAGTTGGGGGAGGTGCAAGAATCAGAAACAAAGATGTTGGGCATCGTGTTGTCTGCAAAGGCTGTAAGGCGCCGATCCGCATTTATAACGACGGTCCCATCAGAGTGTCGGCAACACTTCAACTGCACCTTTCACTTCATATTTATTTCTTTTTCTCTTAAATCATAACTTTTTTTCTTTTTTTATTTCAACCTCTCAACATAAGGATAGAGTTATTGCGTTTTAATTAGTTTATCTTACATTTTTCTAAATATTAA

mRNA sequence

GGCGGTTCCATTTAGGTTAGGAGTAAAAAATTTGTTCGTCCATTTTTCCGTAACCCATCTGTTCTTGGATATACCTCTGTTTTACTTCAATATTCTTATGCTCTGTGGCATTTGAATGTTGATGATGGGCATGGCGGTTCCTTTTACTTGCAATCAGCACTCGTGGATTTCTACCATTTCTGGCCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTATTGGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATCGCGATCGGCTAGAACAAAGTATCTTGAATTCCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATCGCACTCTTTTCTGATAATTTATGCCGATGGCTTGTTGCCGATCAAATATTGTATTTCATTTTGTTATATTTTAATAATTTGAGTTCAGGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACTCTGAAAGGACTTCAGAGTGTTCATTTCAAATTGGACCAATCCTCATTGCCAATGAACCATTCACGGAAGAGAGGTCCCACAAAGTTGGGGGAGGTGCAAGAATCAGAAACAAAGATGTTGGGCATCGTGTTGTCTGCAAAGGCTGTAAGGCGCCGATCCGCATTTATAACGACGGTCCCATCAGAGTGTCGGCAACACTTCAACTGCACCTTTCACTTCATATTTATTTCTTTTTCTCTTAAATCATAACTTTTTTTCTTTTTTTATTTCAACCTCTCAACATAAGGATAGAGTTATTGCGTTTTAATTAGTTTATCTTACATTTTTCTAAATATTAA

Coding sequence (CDS)

ATGTTGATGATGGGCATGGCGGTTCCTTTTACTTGCAATCAGCACTCGTGGATTTCTACCATTTCTGGCCATTCGCTCCAAAGTAGCCCTGATTTTCGAATCTCTTGCCAATCGAAGGTATTGGTTCCTGATATTTGGAGGTCTTCAGCGGAGAGGTATCGCGATCGGCTAGAACAAAGTATCTTGAATTCCTGTGAAGGTCTTAGGGCCATTGGAATAAAGCCAGATGAAAAGATCGCACTCTTTTCTGATAATTTATGCCGATGGCTTGTTGCCGATCAAATATTGTATTTCATTTTGTTATATTTTAATAATTTGAGTTCAGGTATTATGAGTATGGGAGCAATTAATGTTGTTAGAGGTTCAAGATCATCAAGTGAGGAGTTGTTGCAAATATACAATCACTCTGAAAGGACTTCAGAGTGTTCATTTCAAATTGGACCAATCCTCATTGCCAATGAACCATTCACGGAAGAGAGGTCCCACAAAGTTGGGGGAGGTGCAAGAATCAGAAACAAAGATGTTGGGCATCGTGTTGTCTGCAAAGGCTGTAAGGCGCCGATCCGCATTTATAACGACGGTCCCATCAGAGTGTCGGCAACACTTCAACTGCACCTTTCACTTCATATTTATTTCTTTTTCTCTTAA

Protein sequence

MLMMGMAVPFTCNQHSWISTISGHSLQSSPDFRISCQSKVLVPDIWRSSAERYRDRLEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGIMSMGAINVVRGSRSSSEELLQIYNHSERTSECSFQIGPILIANEPFTEERSHKVGGGARIRNKDVGHRVVCKGCKAPIRIYNDGPIRVSATLQLHLSLHIYFFFS
Homology
BLAST of Cp4.1LG17g04020 vs. ExPASy Swiss-Prot
Match: Q8W471 (Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE15 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-24
Identity = 64/114 (56.14%), Postives = 72/114 (63.16%), Query Frame = 0

Query: 42  VPDIWRSSAERYRDR------------------LEQSILNSCEGLRAIGIKPDEKIALFS 101
           VPDIWRSSAE+Y DR                  LEQ IL+  EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 102 DNLCRWLVADQILYFILLYFNNLSSGIMSMGAINVVRGSRSSSEELLQIYNHSE 138
           DN CRWLV+DQ              GIM+ GA+NVVRGSRSS EELLQIY HSE
Sbjct: 159 DNSCRWLVSDQ--------------GIMATGAVNVVRGSRSSVEELLQIYRHSE 198

BLAST of Cp4.1LG17g04020 vs. ExPASy Swiss-Prot
Match: Q9LK39 (Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AAE16 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.7e-24
Identity = 64/114 (56.14%), Postives = 71/114 (62.28%), Query Frame = 0

Query: 42  VPDIWRSSAERYRDR------------------LEQSILNSCEGLRAIGIKPDEKIALFS 101
           VPDIWRSS E+Y DR                  LEQ IL+  EGLR +G+K DEKIALF+
Sbjct: 83  VPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDFVEGLRVVGVKADEKIALFA 142

Query: 102 DNLCRWLVADQILYFILLYFNNLSSGIMSMGAINVVRGSRSSSEELLQIYNHSE 138
           DN CRWLVADQ              GIM+ GA+NVVRGSRSS EELLQIY HSE
Sbjct: 143 DNSCRWLVADQ--------------GIMATGAVNVVRGSRSSVEELLQIYCHSE 182

BLAST of Cp4.1LG17g04020 vs. NCBI nr
Match: XP_022964384.1 (long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 149 bits (376), Expect = 1.63e-42
Identity = 78/106 (73.58%), Postives = 79/106 (74.53%), Query Frame = 0

Query: 1   MLMMGMAVPFTCNQHSWISTISGHSLQSSPDFRISCQSKVLVPDIWRSSAERYRDR---- 60
           MLMMG A+PFTCNQH WISTIS HSLQSSPDFRISCQSK LVPDIWR SAERY DR    
Sbjct: 1   MLMMGAAIPFTCNQHLWISTISRHSLQSSPDFRISCQSKELVPDIWRFSAERYGDRVALL 60

Query: 61  --------------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCR 88
                         LEQ ILN CEGLRAIGIKPDEKIALFSDN CR
Sbjct: 61  DPYHNHASKITYKQLEQIILNFCEGLRAIGIKPDEKIALFSDNSCR 106

BLAST of Cp4.1LG17g04020 vs. NCBI nr
Match: XP_022147167.1 (probable acyl-activating enzyme 16, chloroplastic [Momordica charantia])

HSP 1 Score: 137 bits (346), Expect = 1.98e-33
Identity = 95/206 (46.12%), Postives = 105/206 (50.97%), Query Frame = 0

Query: 4   MGMAVPFTCNQHSWISTISGHSLQ-------------------------SSPDFRISCQS 63
           M MAVP TC+ HSW S+ SG +LQ                         +S D R+ CQS
Sbjct: 1   MAMAVPLTCS-HSWSSSDSGRALQFLFSRYSLTGSYLLKNCVGSGARLSASRDRRVFCQS 60

Query: 64  KV-----------------------------LVPDIWRSSAERYRDR------------- 123
           K                               VPDIWRSSAERY DR             
Sbjct: 61  KTETLQLRRYSPFLESSFVPGNSATVSDEWQTVPDIWRSSAERYGDRVALVDPYHNPASK 120

Query: 124 -----LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGIM 137
                LEQ+IL+  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GIM
Sbjct: 121 MTYKELEQNILSFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GIM 180

BLAST of Cp4.1LG17g04020 vs. NCBI nr
Match: XP_022999933.1 (long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like isoform X4 [Cucurbita maxima])

HSP 1 Score: 125 bits (314), Expect = 4.05e-33
Identity = 73/106 (68.87%), Postives = 73/106 (68.87%), Query Frame = 0

Query: 1  MLMMGMAVPFTCNQHSWISTISGHSLQSSPDFRISCQSKVLVPDIWRSSAERYRDR---- 60
          MLMMG A          ISTIS HSLQSSPDFRISCQSKVLVPDIWRSSAERY DR    
Sbjct: 1  MLMMGAA----------ISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLL 60

Query: 61 --------------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCR 88
                        LEQSILN CEGLRAIGIKPDEKIALFSDN CR
Sbjct: 61 DPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCR 96

BLAST of Cp4.1LG17g04020 vs. NCBI nr
Match: XP_022958898.1 (probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 134 bits (337), Expect = 3.32e-32
Identity = 94/207 (45.41%), Postives = 101/207 (48.79%), Query Frame = 0

Query: 4   MGMAVPFTCNQHSWISTISGHSL-------------------------QSSPDFRISCQS 63
           M MA+PFT +Q SW ST    SL                          ++ D R+ CQS
Sbjct: 1   MAMALPFTSSQLSWRSTDCSQSLPFLFSRYRLTGPYLLQSCVGSGVRRSANRDCRVFCQS 60

Query: 64  KVL------------------------------VPDIWRSSAERYRDR------------ 123
                                            VPDIWRSSAERY DR            
Sbjct: 61  NKTETLQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYHNPAS 120

Query: 124 ------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGI 137
                 LEQSILN  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GI
Sbjct: 121 KMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GI 180

BLAST of Cp4.1LG17g04020 vs. NCBI nr
Match: KAG6575211.1 (putative acyl-activating enzyme 16, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 134 bits (337), Expect = 3.32e-32
Identity = 94/207 (45.41%), Postives = 101/207 (48.79%), Query Frame = 0

Query: 4   MGMAVPFTCNQHSWISTISGHSL-------------------------QSSPDFRISCQS 63
           M MA+PFT +Q SW ST    SL                          ++ D R+ CQS
Sbjct: 1   MAMALPFTSSQLSWRSTDCSQSLPFLFSRYRLTGPYLLQSCVGSGVRRSANRDCRVFCQS 60

Query: 64  KVL------------------------------VPDIWRSSAERYRDR------------ 123
                                            VPDIWRSSAERY DR            
Sbjct: 61  NKTETLQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYHNPAS 120

Query: 124 ------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGI 137
                 LEQSILN  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GI
Sbjct: 121 KMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GI 180

BLAST of Cp4.1LG17g04020 vs. ExPASy TrEMBL
Match: A0A6J1HMZ9 (long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111464417 PE=4 SV=1)

HSP 1 Score: 149 bits (376), Expect = 7.90e-43
Identity = 78/106 (73.58%), Postives = 79/106 (74.53%), Query Frame = 0

Query: 1   MLMMGMAVPFTCNQHSWISTISGHSLQSSPDFRISCQSKVLVPDIWRSSAERYRDR---- 60
           MLMMG A+PFTCNQH WISTIS HSLQSSPDFRISCQSK LVPDIWR SAERY DR    
Sbjct: 1   MLMMGAAIPFTCNQHLWISTISRHSLQSSPDFRISCQSKELVPDIWRFSAERYGDRVALL 60

Query: 61  --------------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCR 88
                         LEQ ILN CEGLRAIGIKPDEKIALFSDN CR
Sbjct: 61  DPYHNHASKITYKQLEQIILNFCEGLRAIGIKPDEKIALFSDNSCR 106

BLAST of Cp4.1LG17g04020 vs. ExPASy TrEMBL
Match: A0A6J1D1M2 (probable acyl-activating enzyme 16, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016178 PE=4 SV=1)

HSP 1 Score: 137 bits (346), Expect = 9.61e-34
Identity = 95/206 (46.12%), Postives = 105/206 (50.97%), Query Frame = 0

Query: 4   MGMAVPFTCNQHSWISTISGHSLQ-------------------------SSPDFRISCQS 63
           M MAVP TC+ HSW S+ SG +LQ                         +S D R+ CQS
Sbjct: 1   MAMAVPLTCS-HSWSSSDSGRALQFLFSRYSLTGSYLLKNCVGSGARLSASRDRRVFCQS 60

Query: 64  KV-----------------------------LVPDIWRSSAERYRDR------------- 123
           K                               VPDIWRSSAERY DR             
Sbjct: 61  KTETLQLRRYSPFLESSFVPGNSATVSDEWQTVPDIWRSSAERYGDRVALVDPYHNPASK 120

Query: 124 -----LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGIM 137
                LEQ+IL+  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GIM
Sbjct: 121 MTYKELEQNILSFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GIM 180

BLAST of Cp4.1LG17g04020 vs. ExPASy TrEMBL
Match: A0A6J1KL42 (long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111494262 PE=4 SV=1)

HSP 1 Score: 125 bits (314), Expect = 1.96e-33
Identity = 73/106 (68.87%), Postives = 73/106 (68.87%), Query Frame = 0

Query: 1  MLMMGMAVPFTCNQHSWISTISGHSLQSSPDFRISCQSKVLVPDIWRSSAERYRDR---- 60
          MLMMG A          ISTIS HSLQSSPDFRISCQSKVLVPDIWRSSAERY DR    
Sbjct: 1  MLMMGAA----------ISTISRHSLQSSPDFRISCQSKVLVPDIWRSSAERYGDRVVLL 60

Query: 61 --------------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCR 88
                        LEQSILN CEGLRAIGIKPDEKIALFSDN CR
Sbjct: 61 DPYHNPASKITYKQLEQSILNFCEGLRAIGIKPDEKIALFSDNSCR 96

BLAST of Cp4.1LG17g04020 vs. ExPASy TrEMBL
Match: A0A6J1H4D5 (probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460048 PE=4 SV=1)

HSP 1 Score: 134 bits (337), Expect = 1.61e-32
Identity = 94/207 (45.41%), Postives = 101/207 (48.79%), Query Frame = 0

Query: 4   MGMAVPFTCNQHSWISTISGHSL-------------------------QSSPDFRISCQS 63
           M MA+PFT +Q SW ST    SL                          ++ D R+ CQS
Sbjct: 1   MAMALPFTSSQLSWRSTDCSQSLPFLFSRYRLTGPYLLQSCVGSGVRRSANRDCRVFCQS 60

Query: 64  KVL------------------------------VPDIWRSSAERYRDR------------ 123
                                            VPDIWRSSAERY DR            
Sbjct: 61  NKTETLQLRRYSPLLESSFVPGNSATVSDEWQAVPDIWRSSAERYGDRVALVDPYHNPAS 120

Query: 124 ------LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGI 137
                 LEQSILN  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GI
Sbjct: 121 KMTYKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GI 180

BLAST of Cp4.1LG17g04020 vs. ExPASy TrEMBL
Match: A0A5D3CUE6 (Putative acyl-activating enzyme 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00130 PE=4 SV=1)

HSP 1 Score: 133 bits (334), Expect = 4.07e-32
Identity = 95/204 (46.57%), Postives = 100/204 (49.02%), Query Frame = 0

Query: 3   MMGMAVPFTCNQHSWIST------------ISGHSLQSSP----------DFRISCQSKV 62
           MM MA  FT  Q SW S+               H LQ+            D R+ CQSK 
Sbjct: 1   MMAMAFSFTPTQLSWTSSDCPRSLHFLFSHYGSHLLQNCVGSGARRLAIRDCRVFCQSKT 60

Query: 63  -----------------------------LVPDIWRSSAERYRDR--------------- 122
                                         VPDIWRSSAERY DR               
Sbjct: 61  ETLQLRRYSPLLESSFVPGTSATVSDEWQAVPDIWRSSAERYGDRIALVDPYHNPASKMT 120

Query: 123 ---LEQSILNSCEGLRAIGIKPDEKIALFSDNLCRWLVADQILYFILLYFNNLSSGIMSM 137
              LEQSILN  EGLRAIGIKPDEKIALF+DN CRWLVADQ              GIM+M
Sbjct: 121 YKELEQSILNFSEGLRAIGIKPDEKIALFADNSCRWLVADQ--------------GIMTM 180

BLAST of Cp4.1LG17g04020 vs. TAIR 10
Match: AT4G14070.1 (acyl-activating enzyme 15 )

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-25
Identity = 64/114 (56.14%), Postives = 72/114 (63.16%), Query Frame = 0

Query: 42  VPDIWRSSAERYRDR------------------LEQSILNSCEGLRAIGIKPDEKIALFS 101
           VPDIWRSSAE+Y DR                  LEQ IL+  EGLR +G+K DEKIALF+
Sbjct: 99  VPDIWRSSAEKYGDRVALVDPYHDPPLKLTYKQLEQEILDFAEGLRVLGVKADEKIALFA 158

Query: 102 DNLCRWLVADQILYFILLYFNNLSSGIMSMGAINVVRGSRSSSEELLQIYNHSE 138
           DN CRWLV+DQ              GIM+ GA+NVVRGSRSS EELLQIY HSE
Sbjct: 159 DNSCRWLVSDQ--------------GIMATGAVNVVRGSRSSVEELLQIYRHSE 198


HSP 2 Score: 38.1 bits (87), Expect = 1.0e-02
Identity = 14/19 (73.68%), Postives = 18/19 (94.74%), Query Frame = 0

Query: 139 TSECSFQIGPILIANEPFT 158
           TSECSFQ+GP+LI ++PFT
Sbjct: 679 TSECSFQVGPVLIVDDPFT 697

BLAST of Cp4.1LG17g04020 vs. TAIR 10
Match: AT3G23790.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 112.8 bits (281), Expect = 3.3e-25
Identity = 64/114 (56.14%), Postives = 71/114 (62.28%), Query Frame = 0

Query: 42  VPDIWRSSAERYRDR------------------LEQSILNSCEGLRAIGIKPDEKIALFS 101
           VPDIWRSS E+Y DR                  LEQ IL+  EGLR +G+K DEKIALF+
Sbjct: 83  VPDIWRSSVEKYGDRVAVVDPYHDPPSTFTYRQLEQEILDFVEGLRVVGVKADEKIALFA 142

Query: 102 DNLCRWLVADQILYFILLYFNNLSSGIMSMGAINVVRGSRSSSEELLQIYNHSE 138
           DN CRWLVADQ              GIM+ GA+NVVRGSRSS EELLQIY HSE
Sbjct: 143 DNSCRWLVADQ--------------GIMATGAVNVVRGSRSSVEELLQIYCHSE 182


HSP 2 Score: 38.1 bits (87), Expect = 1.0e-02
Identity = 14/19 (73.68%), Postives = 18/19 (94.74%), Query Frame = 0

Query: 139 TSECSFQIGPILIANEPFT 158
           TS+CSFQ+GP+LI +EPFT
Sbjct: 674 TSQCSFQVGPVLIVDEPFT 692

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W4711.6e-2456.14Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic OS=Ara... [more]
Q9LK394.7e-2456.14Probable acyl-activating enzyme 16, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
XP_022964384.11.63e-4273.58long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like i... [more]
XP_022147167.11.98e-3346.12probable acyl-activating enzyme 16, chloroplastic [Momordica charantia][more]
XP_022999933.14.05e-3368.87long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like i... [more]
XP_022958898.13.32e-3245.41probable acyl-activating enzyme 16, chloroplastic isoform X1 [Cucurbita moschata... [more]
KAG6575211.13.32e-3245.41putative acyl-activating enzyme 16, chloroplastic, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
A0A6J1HMZ97.90e-4373.58long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like i... [more]
A0A6J1D1M29.61e-3446.12probable acyl-activating enzyme 16, chloroplastic OS=Momordica charantia OX=3673... [more]
A0A6J1KL421.96e-3368.87long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic-like i... [more]
A0A6J1H4D51.61e-3245.41probable acyl-activating enzyme 16, chloroplastic isoform X1 OS=Cucurbita moscha... [more]
A0A5D3CUE64.07e-3246.57Putative acyl-activating enzyme 16 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT4G14070.11.1e-2556.14acyl-activating enzyme 15 [more]
AT3G23790.13.3e-2556.14AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR43813:SF6LONG-CHAIN-FATTY-ACID--[ACYL-CARRIER-PROTEIN] LIGASE AEE15, CHLOROPLASTICcoord: 23..137
NoneNo IPR availablePANTHERPTHR43813ACYL-ACTIVATING ENZYME 16, CHLOROPLASTIC-RELATEDcoord: 23..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG17g04020.1Cp4.1LG17g04020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016874 ligase activity