Cp4.1LG17g03890 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g03890
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	B box-type domain-containing protein
Location	Cp4.1LG17: 2884092 .. 2886044 (+)
RNA-Seq Expression	Cp4.1LG17g03890
Synteny	Cp4.1LG17g03890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATGAGAGGGAAGGAGGAGCGTGTACCCATTTTTACTGTAAAGAAACAGAGAAAAGTCACTGTTTCACGTCTTGTCATTTACCACATGCTTCACTCAAACCAAAATCAAAACCAAAATCAAAATCAAAATCAAAATCAAAATCAAACCTCTCCTCCCTCTTTCACAAAATTCTGAATTACAAAGCAATTGATATGGAAGAAACGTAGGCCATTTTGTTTAGGGTTTTCCTCAAGGATCTTCCGGACATCCATTACATCTCTCTCTCTCTCTCTCTCTCTCTCTTCTCTTTATAATGTCCCATTACTCCTCTCCTCTCATCTCTCCGCCGTCTCTTTCTCTCTCCCTATGCCTTCTTAAACCCTAATCCCTCTCTCTCTCTCTCGTTAAAAAAAAAAAAGAAAAGAAAGAAATGGTGGGTTAATTCTGTTCTCTCTCTCTGTGTCTGTCTCTCTGTTTCTCGCAAGAATTGGTTCTAAATTGCTCTGTTCTTGTTTTTTAATTGTCCGCAGTACACGGTACCCATCTGGGTGCAAGCGCTTCTGAGCGAGAAATTCTTCAACGCCTGTGTGGTTCATGAAGATGCCAAAAAGAACGAGAAAAACATCTTCTGTTTCGACTGTTCCCTCGGTATCTGCCCGCACTGCTTGTCCTCTCACCGCTCTCACAAACTCCTCCAGGTACAATAATTCACAATCCCTAACTCTTTCAACAACCCCCCCTTTGCTCCACAACTCTGTTCAATTCCGCTGTGTTTCCTTTTCAGATTAGGCGATATGTTTATCACGATGTCATTAGATTGGACGATGCTGTGAAGCTAATTGACTGTGCTTTTGTTCAAGTAAGTAAAAATCTTAATTAATTCTCACCATGCTTTTGCAACACACACCCAAAATGTTGATGGGGGGGGTTTTTGTTATTAATAGTCGTACACTACGAATAGTGCGAAAGTGGTGTTTCTGAAACAGAGGCCACAGACTAGAAACTTCAGAGGCTCCTCCGGCAACCTCTGTAGTACCTGCGATAGAAGCCTTCAAGACCCTTATCTCTTTTGCTCTCTCTCTTGCAAGGTTCGATTCGGTTTCTGGTTTTTTTCTCTTCATTTTTTGCTTCTTGTTCTTGTGTTCTAAACTGGGTTTTTGTTTCTTTTCCTTGCCTTGGGTTTCGTTATGTAGATTGATTATCTGGTAAAGGCGCAAGGTGGGGTTTCCAGGCATCTTTTGGAGTGCAATTTCTTGCCATTACCCGAACCGGTTTGGGACGACGGAATGATTATGACGCCTGACTCTGTTCTAGAACCGGCCGGTTCGACCCGAACCTCTTCCAGTTCCGACGGTGGTAGAGTGTTGGAGATGAGGACTACTATTGCTTCGACGGCGACCACGGACTTTGTGAAGAAAAAGAGAAGTAGCTTGACGGCTGCTGCGGCGGCGTATCGGACAACGTGCCGGCCGGCTTGCTGTTCGGTGACGGTTTCCGAGACGTCTGGGAGTCTGATGAGCCGGAGAAAAAGTACCCCGCAGCGGGCCCCACTCTATTGAATTTTCATTTTCTATTTATTTTTCAAGTGTAGTACTGTAGTGGGCAGTCTGTAAGGGGGAGGTTTTTTAGGGACAGAGATGTCATTTTGTGGTTTCTCATGGGGGTGTTTTGGGGATATATGATGAAGTTATTTTTGGTGAGACGGATTTTGATAGTGTGNGGTGGGTTCGTGAGTCGACATGTTTTTGGTTGGGTCAACCCAATTTACCCGATTCATTAAAAAACAGGGTTAGAATCGAAGTCTACCGTTAACATATAAGAGTAAAAAAAAAAGTGATGGATTTGATGAAAAGAATTGAAGTATGAAGGTGGATGGAATGAGAGGGAAGGAGGAGCGTGTACCCATTTTTACTGTAAAGAAACAGAGAAAAGTCACTGTTTCACGTCTTGTCATTTACCACATGCTTCACTCA

mRNA sequence

AATGAGAGGGAAGGAGGAGCGTGTACCCATTTTTACTGTAAAGAAACAGAGAAAAGTCACTGTTTCACGTCTTGTCATTTACCACATGCTTCACTCAAACCAAAATCAAAACCAAAATCAAAATCAAAATCAAAATCAAAATCAAACCTCTCCTCCCTCTTTCACAAAATTCTGAATTACAAAGCAATTGATATGGAAGAAACGTAGGCCATTTTGTTTAGGGTTTTCCTCAAGGATCTTCCGGACATCCATTACATCTCTCTCTCTCTCTCTCTCTCTCTCTTCTCTTTATAATGTCCCATTACTCCTCTCCTCTCATCTCTCCGCCGTCTCTTTCTCTCTCCCTATGCCTTCTTAAACCCTAATCCCTCTCTCTCTCTCTCGTTAAAAAAAAAAAAGAAAAGAAAGAAATGTACACGGTACCCATCTGGGTGCAAGCGCTTCTGAGCGAGAAATTCTTCAACGCCTGTGTGGTTCATGAAGATGCCAAAAAGAACGAGAAAAACATCTTCTGTTTCGACTGTTCCCTCGGTATCTGCCCGCACTGCTTGTCCTCTCACCGCTCTCACAAACTCCTCCAGATTAGGCGATATGTTTATCACGATGTCATTAGATTGGACGATGCTGTGAAGCTAATTGACTGTGCTTTTGTTCAATCGTACACTACGAATAGTGCGAAAGTGGTGTTTCTGAAACAGAGGCCACAGACTAGAAACTTCAGAGGCTCCTCCGGCAACCTCTGTAGTACCTGCGATAGAAGCCTTCAAGACCCTTATCTCTTTTGCTCTCTCTCTTGCAAGATTGATTATCTGGTAAAGGCGCAAGGTGGGGTTTCCAGGCATCTTTTGGAGTGCAATTTCTTGCCATTACCCGAACCGGTTTGGGACGACGGAATGATTATGACGCCTGACTCTGTTCTAGAACCGGCCGGTTCGACCCGAACCTCTTCCAGTTCCGACGGTGGTAGAGTGTTGGAGATGAGGACTACTATTGCTTCGACGGCGACCACGGACTTTGTGAAGAAAAAGAGAAGTAGCTTGACGGCTGCTGCGGCGGCGTATCGGACAACGTGCCGGCCGGCTTGCTGTTCGGTGACGGTTTCCGAGACGTCTGGGAGTCTGATGAGCCGGAGAAAAAGTACCCCGCAGCGGGCCCCACTCTATTGAATTTTCATTTTCTATTTATTTTTCAAGTGTAGTACTGTAGTGGGCAGTCTGTAAGGGGGAGGTTTTTTAGGGACAGAGATGTCATTTTGTGGTTTCTCATGGGGGTGTTTTGGGGATATATGATGAAGTTATTTTTGGTGAGACGGATTTTGATAGTGTGNGGTGGGTTCGTGAGTCGACATGTTTTTGGTTGGGTCAACCCAATTTACCCGATTCATTAAAAAACAGGGTTAGAATCGAAGTCTACCGTTAACATATAAGAGTAAAAAAAAAAGTGATGGATTTGATGAAAAGAATTGAAGTATGAAGGTGGATGGAATGAGAGGGAAGGAGGAGCGTGTACCCATTTTTACTGTAAAGAAACAGAGAAAAGTCACTGTTTCACGTCTTGTCATTTACCACATGCTTCACTCA

Coding sequence (CDS)

ATGTACACGGTACCCATCTGGGTGCAAGCGCTTCTGAGCGAGAAATTCTTCAACGCCTGTGTGGTTCATGAAGATGCCAAAAAGAACGAGAAAAACATCTTCTGTTTCGACTGTTCCCTCGGTATCTGCCCGCACTGCTTGTCCTCTCACCGCTCTCACAAACTCCTCCAGATTAGGCGATATGTTTATCACGATGTCATTAGATTGGACGATGCTGTGAAGCTAATTGACTGTGCTTTTGTTCAATCGTACACTACGAATAGTGCGAAAGTGGTGTTTCTGAAACAGAGGCCACAGACTAGAAACTTCAGAGGCTCCTCCGGCAACCTCTGTAGTACCTGCGATAGAAGCCTTCAAGACCCTTATCTCTTTTGCTCTCTCTCTTGCAAGATTGATTATCTGGTAAAGGCGCAAGGTGGGGTTTCCAGGCATCTTTTGGAGTGCAATTTCTTGCCATTACCCGAACCGGTTTGGGACGACGGAATGATTATGACGCCTGACTCTGTTCTAGAACCGGCCGGTTCGACCCGAACCTCTTCCAGTTCCGACGGTGGTAGAGTGTTGGAGATGAGGACTACTATTGCTTCGACGGCGACCACGGACTTTGTGAAGAAAAAGAGAAGTAGCTTGACGGCTGCTGCGGCGGCGTATCGGACAACGTGCCGGCCGGCTTGCTGTTCGGTGACGGTTTCCGAGACGTCTGGGAGTCTGATGAGCCGGAGAAAAAGTACCCCGCAGCGGGCCCCACTCTATTGA

Protein sequence

MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRYVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDPYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSSSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRRKSTPQRAPLY

Homology

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BLAST of Cp4.1LG17g03890 vs. ExPASy Swiss-Prot
Match: Q1G3Q4 (Protein RGF1 INDUCIBLE TRANSCRIPTION FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=RITF1 PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 9.6e-53
Identity = 113/258 (43.80%), Postives = 155/258 (60.08%), Query Frame = 0


Query: 5   PIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRYVYH 64
           P W+ AL +EKFF  C  HE AKKNE+N+ C DC   +CPHC+ SHR H+LLQ+RRYVYH
Sbjct: 6   PAWLDALYAEKFFVGCPYHETAKKNERNVCCLDCCTSLCPHCVPSHRFHRLLQVRRYVYH 65

Query: 65  DVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDPYLF 124
           DV+RL+D  KLIDC+ VQ+YT NSAKVVF+K+RPQ R F+G +GN C++CDRSLQ+PY+ 
Sbjct: 66  DVVRLEDLQKLIDCSNVQAYTINSAKVVFIKKRPQNRQFKG-AGNYCTSCDRSLQEPYIH 125

Query: 125 CSLSCKIDYLVKAQGGVSRHLLECNFLPL------PEPVWDDGMIM---TPDSVLEPAGS 184
           CSL CK+D+++K    ++  L  C+ L L      P+ +  D  +    TP S +     
Sbjct: 126 CSLGCKVDFVMKRYRDITPFLKPCHTLTLGPDYIIPQDLLTDDEVAAYETPRSTVVDGDE 185

Query: 185 TRTSSSSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSG 244
           + + SS+             +  TT  V+KKR+     A       + A     VSE   
Sbjct: 186 SMSWSSASSDNNNAGAAAAYAATTTHVVRKKRTGFCLCA-------KSANSYKEVSEDPD 245

Query: 245 SL---MSRRKSTPQRAPL 251
            +   ++RRK  PQR+PL
Sbjct: 246 DISACINRRKGVPQRSPL 255

[Close]

BLAST of Cp4.1LG17g03890 vs. NCBI nr
Match: XP_023514505.1 (uncharacterized protein LOC111778763 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504 bits (1297), Expect = 6.31e-180
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR
Sbjct: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS
Sbjct: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
           SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR
Sbjct: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RKSTPQRAPLY
Sbjct: 241 RKSTPQRAPLY 251

[Close]

BLAST of Cp4.1LG17g03890 vs. NCBI nr
Match: XP_022964357.1 (uncharacterized protein LOC111464390 [Cucurbita moschata])

HSP 1 Score: 501 bits (1289), Expect = 1.05e-178
Identity = 249/251 (99.20%), Postives = 250/251 (99.60%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           MYTVPIW+Q LLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR
Sbjct: 1   MYTVPIWLQPLLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS
Sbjct: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
           SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR
Sbjct: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RKSTPQRAPLY
Sbjct: 241 RKSTPQRAPLY 251

[Close]

BLAST of Cp4.1LG17g03890 vs. NCBI nr
Match: KAG7025963.1 (hypothetical protein SDJN02_12461, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501 bits (1289), Expect = 2.18e-178
Identity = 249/250 (99.60%), Postives = 250/250 (100.00%), Query Frame = 0


Query: 2   YTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY 61
           YTVPIW+QALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY
Sbjct: 22  YTVPIWLQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY 81

Query: 62  VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP 121
           VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP
Sbjct: 82  VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP 141

Query: 122 YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS 181
           YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS
Sbjct: 142 YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS 201

Query: 182 SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR 241
           SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR
Sbjct: 202 SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR 261

Query: 242 KSTPQRAPLY 251
           KSTPQRAPLY
Sbjct: 262 KSTPQRAPLY 271

[Close]

BLAST of Cp4.1LG17g03890 vs. NCBI nr
Match: KAG6593617.1 (hypothetical protein SDJN03_13093, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 501 bits (1289), Expect = 2.18e-178
Identity = 249/250 (99.60%), Postives = 250/250 (100.00%), Query Frame = 0


Query: 2   YTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY 61
           YTVPIW+QALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY
Sbjct: 22  YTVPIWLQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRY 81

Query: 62  VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP 121
           VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP
Sbjct: 82  VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP 141

Query: 122 YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS 181
           YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS
Sbjct: 142 YLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSS 201

Query: 182 SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR 241
           SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR
Sbjct: 202 SDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSRR 261

Query: 242 KSTPQRAPLY 251
           KSTPQRAPLY
Sbjct: 262 KSTPQRAPLY 271

[Close]

BLAST of Cp4.1LG17g03890 vs. NCBI nr
Match: XP_023000095.1 (uncharacterized protein LOC111494393 [Cucurbita maxima])

HSP 1 Score: 486 bits (1252), Expect = 4.58e-173
Identity = 242/251 (96.41%), Postives = 245/251 (97.61%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           MY +PIW+QALLSEKFFNACV+HEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR
Sbjct: 1   MYALPIWLQALLSEKFFNACVLHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PY FCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEP WDDGMIMTPDSVLEPAGSTRTSS
Sbjct: 121 PYHFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPAWDDGMIMTPDSVLEPAGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
           SSDGGR LEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPA CSVTVSETSGSLMSR
Sbjct: 181 SSDGGRALEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPARCSVTVSETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RK TPQRAPLY
Sbjct: 241 RKGTPQRAPLY 251

[Close]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL
Match: A0A6J1HHL3 (uncharacterized protein LOC111464390 OS=Cucurbita moschata OX=3662 GN=LOC111464390 PE=4 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 5.07e-179
Identity = 249/251 (99.20%), Postives = 250/251 (99.60%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           MYTVPIW+Q LLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR
Sbjct: 1   MYTVPIWLQPLLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS
Sbjct: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
           SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR
Sbjct: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RKSTPQRAPLY
Sbjct: 241 RKSTPQRAPLY 251

[Close]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL
Match: A0A6J1KHE0 (uncharacterized protein LOC111494393 OS=Cucurbita maxima OX=3661 GN=LOC111494393 PE=4 SV=1)

HSP 1 Score: 486 bits (1252), Expect = 2.22e-173
Identity = 242/251 (96.41%), Postives = 245/251 (97.61%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           MY +PIW+QALLSEKFFNACV+HEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR
Sbjct: 1   MYALPIWLQALLSEKFFNACVLHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PY FCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEP WDDGMIMTPDSVLEPAGSTRTSS
Sbjct: 121 PYHFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPAWDDGMIMTPDSVLEPAGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
           SSDGGR LEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPA CSVTVSETSGSLMSR
Sbjct: 181 SSDGGRALEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPARCSVTVSETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RK TPQRAPLY
Sbjct: 241 RKGTPQRAPLY 251

[Close]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL
Match: A0A0A0KCM1 (B box-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G109690 PE=4 SV=1)

HSP 1 Score: 435 bits (1119), Expect = 3.85e-153
Identity = 215/251 (85.66%), Postives = 228/251 (90.84%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           M ++PIW+QALL+EKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSH SHKLLQIRR
Sbjct: 1   MCSLPIWLQALLTEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHSSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDA KLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDATKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCS+SCKIDYLVK QGG+S+HLLECNFLPLPEP WDDG++MTPDSVLEP GS R+SS
Sbjct: 121 PYLFCSVSCKIDYLVKTQGGISKHLLECNFLPLPEPGWDDGLMMTPDSVLEPVGSNRSSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
            SDGG       T+ STATT+FVKKKRSSLTAAAAAYR  CRP C   T+SETSGSLMSR
Sbjct: 181 GSDGGGTEGK--TLVSTATTEFVKKKRSSLTAAAAAYRAACRPVCWPGTISETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RK TPQRAPLY
Sbjct: 241 RKGTPQRAPLY 249

[Close]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL
Match: A0A1S3C8E3 (uncharacterized protein LOC103497663 OS=Cucumis melo OX=3656 GN=LOC103497663 PE=4 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 9.83e-151
Identity = 214/251 (85.26%), Postives = 227/251 (90.44%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           M ++PIW+QALL+EKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSH SHKLLQIRR
Sbjct: 1   MCSLPIWLQALLTEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHSSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDA KLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDATKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCS+SCKIDYLVK QGG+S++LLECNFLPLPEP WDDG++MTPDSVLEP GSTRTSS
Sbjct: 121 PYLFCSVSCKIDYLVKTQGGISKYLLECNFLPLPEPGWDDGLMMTPDSVLEPVGSTRTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
            SDGG       T+ STATT+FVKKKRSSLTAAA  YR  CRP C   T+SETSGSLMSR
Sbjct: 181 GSDGGGTEGK--TLVSTATTEFVKKKRSSLTAAA--YRAACRPVCWPGTISETSGSLMSR 240

Query: 241 RKSTPQRAPLY 251
           RK TPQRAPLY
Sbjct: 241 RKGTPQRAPLY 247

[Close]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL
Match: A0A6J1H7K0 (uncharacterized protein LOC111460295 OS=Cucurbita moschata OX=3662 GN=LOC111460295 PE=4 SV=1)

HSP 1 Score: 404 bits (1037), Expect = 9.67e-141
Identity = 203/251 (80.88%), Postives = 220/251 (87.65%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           M+++PIW+QALL+ KFFNACV+HE AKKNEKNIFCFDCSLGICPHCLSSH SHKLLQIRR
Sbjct: 1   MHSLPIWLQALLTHKFFNACVLHEHAKKNEKNIFCFDCSLGICPHCLSSHSSHKLLQIRR 60

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDVIRLDDA KLIDCAFVQSYTTNSAKVVFL QRPQTRNFRGSSGNLCSTCDRSLQD
Sbjct: 61  YVYHDVIRLDDATKLIDCAFVQSYTTNSAKVVFLNQRPQTRNFRGSSGNLCSTCDRSLQD 120

Query: 121 PYLFCSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSS 180
           PYLFCSLSCKIDYL+K +GGVSR+LLECNF       WDDG++MTPDSVLE  GS +TSS
Sbjct: 121 PYLFCSLSCKIDYLMKTEGGVSRNLLECNF-----SAWDDGLMMTPDSVLELGGSNQTSS 180

Query: 181 SSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSGSLMSR 240
            SDGG V   R+ +ASTATT+FVKKKRSSLTAA   YR  CRPAC   T+SETSGSL+SR
Sbjct: 181 GSDGGGVEGGRSVVASTATTEFVKKKRSSLTAA---YRAACRPACWPGTISETSGSLLSR 240

Query: 241 RKSTPQRAPLY 251
           RK TPQRAPLY
Sbjct: 241 RKGTPQRAPLY 243

[Close]

BLAST of Cp4.1LG17g03890 vs. TAIR 10
Match: AT3G60670.1 (PLATZ transcription factor family protein )

HSP 1 Score: 268.9 bits (686), Expect = 4.2e-72
Identity = 143/251 (56.97%), Postives = 173/251 (68.92%), Query Frame = 0


Query: 5   PIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRYVYH 64
           P W++ LL +KFFNAC+ HED KKNEKNI C DC L ICPHCLSSH SH+LLQIRRYVY 
Sbjct: 7   PAWLEVLLKDKFFNACLDHEDDKKNEKNILCIDCCLTICPHCLSSHTSHRLLQIRRYVYR 66

Query: 65  DVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDPYLF 124
           DV+R++D  KL+DC+ +Q YTTNS+KVVF+ +RPQ+R FRG SGN+C TCDRSLQ PYLF
Sbjct: 67  DVLRVEDGSKLMDCSLIQPYTTNSSKVVFINERPQSRQFRG-SGNICITCDRSLQSPYLF 126

Query: 125 CSLSCKIDYLVKAQGGVSRHLLECNFLPLPEPVWDDGMIMTPDSVLEPAGSTRTSSSSDG 184
           C LSCKI  ++  Q G+S  L  CN L L     D+    TP S LEP GS RTSS S G
Sbjct: 127 CCLSCKISDVIMRQRGLSGFLRVCNVLDLT----DEVTTTTPSSTLEPTGSNRTSSESSG 186

Query: 185 --GRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCR--PACCSVTVSETSGSLMSR 244
             G  +     +A TATT+ V+KKRSSL+       TTCR      S T +E   + ++R
Sbjct: 187 NEGEDMFWCQALACTATTEIVRKKRSSLS-------TTCRRVTEVVSTTNTEAPVNFLNR 245

Query: 245 RKSTPQRAPLY 252
           RK+ PQRAPLY
Sbjct: 247 RKNPPQRAPLY 245

[Close]

BLAST of Cp4.1LG17g03890 vs. TAIR 10
Match: AT2G12646.1 (PLATZ transcription factor family protein )

HSP 1 Score: 208.4 bits (529), Expect = 6.8e-54
Identity = 113/258 (43.80%), Postives = 155/258 (60.08%), Query Frame = 0


Query: 5   PIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRRYVYH 64
           P W+ AL +EKFF  C  HE AKKNE+N+ C DC   +CPHC+ SHR H+LLQ+RRYVYH
Sbjct: 6   PAWLDALYAEKFFVGCPYHETAKKNERNVCCLDCCTSLCPHCVPSHRFHRLLQVRRYVYH 65

Query: 65  DVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDPYLF 124
           DV+RL+D  KLIDC+ VQ+YT NSAKVVF+K+RPQ R F+G +GN C++CDRSLQ+PY+ 
Sbjct: 66  DVVRLEDLQKLIDCSNVQAYTINSAKVVFIKKRPQNRQFKG-AGNYCTSCDRSLQEPYIH 125

Query: 125 CSLSCKIDYLVKAQGGVSRHLLECNFLPL------PEPVWDDGMIM---TPDSVLEPAGS 184
           CSL CK+D+++K    ++  L  C+ L L      P+ +  D  +    TP S +     
Sbjct: 126 CSLGCKVDFVMKRYRDITPFLKPCHTLTLGPDYIIPQDLLTDDEVAAYETPRSTVVDGDE 185

Query: 185 TRTSSSSDGGRVLEMRTTIASTATTDFVKKKRSSLTAAAAAYRTTCRPACCSVTVSETSG 244
           + + SS+             +  TT  V+KKR+     A       + A     VSE   
Sbjct: 186 SMSWSSASSDNNNAGAAAAYAATTTHVVRKKRTGFCLCA-------KSANSYKEVSEDPD 245

Query: 245 SL---MSRRKSTPQRAPL 251
            +   ++RRK  PQR+PL
Sbjct: 246 DISACINRRKGVPQRSPL 255

[Close]

BLAST of Cp4.1LG17g03890 vs. TAIR 10
Match: AT1G31040.1 (PLATZ transcription factor family protein )

HSP 1 Score: 185.7 bits (470), Expect = 4.7e-47
Identity = 82/134 (61.19%), Postives = 103/134 (76.87%), Query Frame = 0


Query: 1   MYTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLGICPHCLSSHRSHKLLQIRR 60
           M T P W++ L++E FF++C +HE  +K+EKN+FC  C L +CPHCL SHRSH LLQ+RR
Sbjct: 15  MATKPAWLEGLMAETFFSSCGIHETRRKSEKNVFCLLCCLSVCPHCLPSHRSHPLLQVRR 74

Query: 61  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 120
           YVYHDV+RL D  KLIDC++VQ YT N AKV+FL QR Q+R     S N+C TCDR LQ+
Sbjct: 75  YVYHDVVRLSDLEKLIDCSYVQPYTINGAKVIFLNQRQQSR--AKVSSNVCFTCDRILQE 134

Query: 121 PYLFCSLSCKIDYL 135
           P+ FCSLSCK+DYL
Sbjct: 135 PFHFCSLSCKVDYL 146

[Close]

BLAST of Cp4.1LG17g03890 vs. TAIR 10
Match: AT1G32700.1 (PLATZ transcription factor family protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-32
Identity = 68/135 (50.37%), Postives = 87/135 (64.44%), Query Frame = 0


Query: 3   TVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCSLG-ICPHCLSSHRSHKLLQIRRY 62
           T P W++ LL EKFF  C +H D+ K+E N++C DC+ G +C  CLS H+ H  +QIRR 
Sbjct: 10  TYPHWLKPLLREKFFVQCKLHADSHKSECNMYCLDCTNGPLCSLCLSFHKDHHAIQIRRS 69

Query: 63  VYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQDP 122
            YHDVIR+ +  K +D   VQ+Y  NSAKVVFL +RPQ R  +G   N C  C RSL D 
Sbjct: 70  SYHDVIRVSEIQKFLDITGVQTYVINSAKVVFLNERPQPRPGKGVI-NTCEVCYRSLVDS 129

Query: 123 YLFCSLSCKIDYLVK 137
           + FCSL CKI  + K
Sbjct: 130 FRFCSLGCKISGISK 143

[Close]

BLAST of Cp4.1LG17g03890 vs. TAIR 10
Match: AT1G21000.1 (PLATZ transcription factor family protein )

HSP 1 Score: 135.6 bits (340), Expect = 5.6e-32
Identity = 67/150 (44.67%), Postives = 92/150 (61.33%), Query Frame = 0


Query: 2   YTVPIWVQALLSEKFFNACVVHEDAKKNEKNIFCFDCS-LGICPHCLSSHRSHKLLQIRR 61
           YT P W+  +L   +F  C +H D+ KNE N+FC DC+    C +CL  H+ H+++QIRR
Sbjct: 13  YTSPPWLMPMLRGSYFVPCSIHVDSNKNECNLFCLDCAGNAFCSYCLVKHKDHRVVQIRR 72

Query: 62  YVYHDVIRLDDAVKLIDCAFVQSYTTNSAKVVFLKQRPQTRNFRGSSGNLCSTCDRSLQD 121
             YH+V+R+++  K ID A VQ+Y  NSAK+VFL +RPQ R  +G + N C  C RSL D
Sbjct: 73  SSYHNVVRVNEIQKFIDIACVQTYIINSAKIVFLNERPQPRIGKGVT-NTCEICCRSLLD 132

Query: 122 PYLFCSLSCKIDYLVKAQGGVSRHLLECNF 151
            + FCSL CK+       GG+ R  L   F
Sbjct: 133 SFRFCSLGCKL-------GGMRRGDLSLTF 154

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g03890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1G3Q4	9.6e-53	43.80	Protein RGF1 INDUCIBLE TRANSCRIPTION FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of Cp4.1LG17g03890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514505.1	6.31e-180	100.00	uncharacterized protein LOC111778763 [Cucurbita pepo subsp. pepo]	[more]
XP_022964357.1	1.05e-178	99.20	uncharacterized protein LOC111464390 [Cucurbita moschata]	[more]
KAG7025963.1	2.18e-178	99.60	hypothetical protein SDJN02_12461, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6593617.1	2.18e-178	99.60	hypothetical protein SDJN03_13093, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023000095.1	4.58e-173	96.41	uncharacterized protein LOC111494393 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG17g03890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HHL3	5.07e-179	99.20	uncharacterized protein LOC111464390 OS=Cucurbita moschata OX=3662 GN=LOC1114643...	[more]
A0A6J1KHE0	2.22e-173	96.41	uncharacterized protein LOC111494393 OS=Cucurbita maxima OX=3661 GN=LOC111494393...	[more]
A0A0A0KCM1	3.85e-153	85.66	B box-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G109690 ...	[more]
A0A1S3C8E3	9.83e-151	85.26	uncharacterized protein LOC103497663 OS=Cucumis melo OX=3656 GN=LOC103497663 PE=...	[more]
A0A6J1H7K0	9.67e-141	80.88	uncharacterized protein LOC111460295 OS=Cucurbita moschata OX=3662 GN=LOC1114602...	[more]

BLAST of Cp4.1LG17g03890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G60670.1	4.2e-72	56.97	PLATZ transcription factor family protein	[more]
AT2G12646.1	6.8e-54	43.80	PLATZ transcription factor family protein	[more]
AT1G31040.1	4.7e-47	61.19	PLATZ transcription factor family protein	[more]
AT1G32700.1	3.3e-32	50.37	PLATZ transcription factor family protein	[more]
AT1G21000.1	5.6e-32	44.67	PLATZ transcription factor family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006734	PLATZ transcription factor	PFAM	PF04640	PLATZ	coord: 59..131 e-value: 1.1E-25 score: 90.1
None	No IPR available	PANTHER	PTHR31065	PLATZ TRANSCRIPTION FACTOR FAMILY PROTEIN	coord: 5..251
None	No IPR available	PANTHER	PTHR31065:SF78	SUBFAMILY NOT NAMED	coord: 5..251
None	No IPR available	SUPERFAMILY	57845	B-box zinc-binding domain	coord: 18..63
IPR000315	B-box-type zinc finger	PROSITE	PS50119	ZF_BBOX	coord: 20..58 score: 9.299861

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g03890.1	Cp4.1LG17g03890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding