Cp4.1LG17g02200 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG17g02200 vs. ExPASy Swiss-Prot
Match: P26369 (Splicing factor U2AF 65 kDa subunit OS=Mus musculus OX=10090 GN=U2af2 PE=1 SV=3)

HSP 1 Score: 133.7 bits (335), Expect = 1.1e-29
Identity = 97/321 (30.22%), Postives = 157/321 (48.91%), Query Frame = 0

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           +Q TR  RRLY+ N+P   +E+A++D  N  +   G+    G  P ++  I++D+  A +
Sbjct: 142 SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDKNFAFL 201

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EF + ++ + A++FDG  F G  L+IRRP DY  L                        G
Sbjct: 202 EFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPL-----------------------PG 261

Query: 541 DLEK-SVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIV 600
             E  SV V   +S VV DS +K+ + G+ N ++ +                  Q+K ++
Sbjct: 262 MSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDD------------------QVKELL 321

Query: 601 AAFGPLKAYHF--EINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPA---- 660
            +FGPLKA++   +    L++  AF EYVD +V  +A AGLNGM++G + L V  A    
Sbjct: 322 TSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGA 381

Query: 661 --VPIASLER--NGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLP--LLSESDINEVL 720
               + SL    N       +P  +   +Q      +V      VLP  LL + +  E++
Sbjct: 382 KNATLVSLPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIV 420

Query: 721 EDIRFECARFGTVKSMNVAKP 729
           ED+R EC+++G VKS+ + +P
Sbjct: 442 EDVRDECSKYGLVKSIEIPRP 420

BLAST of Cp4.1LG17g02200 vs. ExPASy Swiss-Prot
Match: Q24562 (Splicing factor U2AF 50 kDa subunit OS=Drosophila melanogaster OX=7227 GN=U2af50 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.3e-29
Identity = 95/334 (28.44%), Postives = 160/334 (47.90%), Query Frame = 0

Query: 424 TRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALVEFL 483
           TR  RRLY+ N+P   +E+ +++  N  +   G+    G+ P ++C I+ D+  A +EF 
Sbjct: 89  TRQARRLYVGNIPFGVTEEEMMEFFNQQMHLVGLAQAAGS-PVLACQINLDKNFAFLEFR 148

Query: 484 TPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTGDLE 543
           + ++ + A++FDG +  G  L+IRRP DY                  Q +     T  ++
Sbjct: 149 SIDETTQAMAFDGINLKGQSLKIRRPHDY------------------QPMPGITDTPAIK 208

Query: 544 KSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVAAFG 603
            +V     IS VV DSP+KI + G+ N ++ +                  Q+K ++ +FG
Sbjct: 209 PAVVSSGVISTVVPDSPHKIFIGGLPNYLNDD------------------QVKELLLSFG 268

Query: 604 PLKAYHF--EINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLER 663
            L+A++   +    L++  AF EYVD S+  ++ AGLNGM++G + L V  A   A   +
Sbjct: 269 KLRAFNLVKDAATGLSKGYAFCEYVDLSITDQSIAGLNGMQLGDKKLIVQRASVGAKNAQ 328

Query: 664 NGCQPCYGIPEHVKPLLQ-----QPTVVL-KVNVFNADVLPLLSESDINEVLEDIRFECA 723
           N       +   V  L        PT VL  +N+   D   L  E +  ++LEDI+ EC 
Sbjct: 329 NAANTTQSVMLQVPGLSNVVTSGPPTEVLCLLNMVTPD--ELRDEEEYEDILEDIKEECT 383

Query: 724 RFGTVKSMNVAKP-----CTSCVSAEEEYKSICD 745
           ++G V+S+ + +P        C     E+ S+ D
Sbjct: 389 KYGVVRSVEIPRPIEGVEVPGCGKVFVEFNSVLD 383

BLAST of Cp4.1LG17g02200 vs. ExPASy Swiss-Prot
Match: P26368 (Splicing factor U2AF 65 kDa subunit OS=Homo sapiens OX=9606 GN=U2AF2 PE=1 SV=4)

HSP 1 Score: 131.7 bits (330), Expect = 4.3e-29
Identity = 96/323 (29.72%), Postives = 158/323 (48.92%), Query Frame = 0

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           +Q TR  RRLY+ N+P   +E+A++D  N  +   G+    G  P ++  I++D+  A +
Sbjct: 142 SQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPG-NPVLAVQINQDKNFAFL 201

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EF + ++ + A++FDG  F G  L+IRRP DY  L                        G
Sbjct: 202 EFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPL-----------------------PG 261

Query: 541 DLEK-SVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIV 600
             E  SV V   +S VV DS +K+ + G+ N ++ +                  Q+K ++
Sbjct: 262 MSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDD------------------QVKELL 321

Query: 601 AAFGPLKAYHF--EINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPA---- 660
            +FGPLKA++   +    L++  AF EYVD +V  +A AGLNGM++G + L V  A    
Sbjct: 322 TSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGA 381

Query: 661 ------VPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLP--LLSESDINE 720
                  P +++  N       +P  +   +Q      +V      VLP  LL + +  E
Sbjct: 382 KNATLVSPPSTI--NQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEE 420

Query: 721 VLEDIRFECARFGTVKSMNVAKP 729
           ++ED+R EC+++G VKS+ + +P
Sbjct: 442 IVEDVRDECSKYGLVKSIEIPRP 420

BLAST of Cp4.1LG17g02200 vs. ExPASy Swiss-Prot
Match: P90978 (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis elegans OX=6239 GN=uaf-1 PE=2 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 2.8e-28
Identity = 86/308 (27.92%), Postives = 154/308 (50.00%), Query Frame = 0

Query: 428 RRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALVEFLTPED 487
           RRLY+ N+P   +E+A++D  N  +   G+    G  P + C I+ D+  A +EF + ++
Sbjct: 184 RRLYVGNIPFGCNEEAMLDFFNQQMHLCGLAQAPG-NPILLCQINLDKNFAFIEFRSIDE 243

Query: 488 ASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTGDLEKSVA 547
            +A ++FDG +F G  L++RRP+DY                          T D+   + 
Sbjct: 244 TTAGMAFDGINFMGQQLKVRRPRDYQP---------------------SQNTFDMNSRMP 303

Query: 548 VVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVAAFGPLKA 607
           V    S +V DS NKI + G+ N ++ +                  Q+K ++ +FGPLKA
Sbjct: 304 V----STIVVDSANKIFIGGLPNYLTED------------------QVKELLCSFGPLKA 363

Query: 608 YHFEINNDLN-EPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLER----- 667
           +   +++  N +  AF EY+D ++  +A AGLNGM++G + L V   +  A+ +R     
Sbjct: 364 FSLNVDSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVV--QLACANQQRHNTNL 423

Query: 668 -NGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 727
            N      GI +  +   +   ++  +N+   D   L ++ +  E+LED+R EC+++G V
Sbjct: 424 PNSASAIAGI-DLSQGAGRATEILCLMNMVTED--ELKADDEYEEILEDVRDECSKYGIV 442

Query: 728 KSMNVAKP 729
           +S+ + +P
Sbjct: 484 RSLEIPRP 442

BLAST of Cp4.1LG17g02200 vs. ExPASy Swiss-Prot
Match: P90727 (Splicing factor U2AF 65 kDa subunit OS=Caenorhabditis briggsae OX=6238 GN=uaf-1 PE=3 SV=2)

HSP 1 Score: 128.6 bits (322), Expect = 3.6e-28
Identity = 86/306 (28.10%), Postives = 150/306 (49.02%), Query Frame = 0

Query: 428 RRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALVEFLTPED 487
           RRLY+ N+P   +E+A++D  N  +    +    G  P + C I+ D+  A +EF + ++
Sbjct: 175 RRLYVGNIPFGCNEEAMLDFFNQQMHLCNLAQAPG-NPILLCQINLDKNFAFIEFRSIDE 234

Query: 488 ASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTGDLEKSVA 547
            +A ++FDG +F G  L++RRP+DY                          T D+   + 
Sbjct: 235 TTAGMAFDGINFMGQQLKVRRPRDYQP---------------------SQNTFDMNARMP 294

Query: 548 VVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVAAFGPLKA 607
           V    S +V DS NKI + G+ N ++ +                  Q+K ++ +FGPLKA
Sbjct: 295 V----SSIVVDSANKIFIGGLPNYLTED------------------QVKELLCSFGPLKA 354

Query: 608 YHFEINNDLN-EPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLER----N 667
           +   +++  N +  AF EY+D ++  +A AGLNGM++G + L V  A    +       N
Sbjct: 355 FSLNVDSQGNSKGYAFAEYLDPTLTDQAIAGLNGMQLGDKQLVVQLACANQTRHNTHLPN 414

Query: 668 GCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTVKS 727
                 GI +  +   +   ++  +N+   D   L S+ D  E+LED+R EC+++G V+S
Sbjct: 415 SASAIAGI-DLSQGAGRATEILCLMNMVTED--ELRSDEDYEEILEDVREECSKYGIVRS 433

Query: 728 MNVAKP 729
           + + +P
Sbjct: 475 LEIPRP 433

BLAST of Cp4.1LG17g02200 vs. NCBI nr
Match: XP_023514084.1 (splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo] >XP_023514085.1 splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo] >XP_023514086.1 splicing factor U2af large subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1728 bits (4476), Expect = 0.0
Identity = 910/950 (95.79%), Postives = 910/950 (95.79%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD
Sbjct: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE
Sbjct: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG
Sbjct: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM                  LKNIVA
Sbjct: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE
Sbjct: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV
Sbjct: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 910

BLAST of Cp4.1LG17g02200 vs. NCBI nr
Match: XP_022964139.1 (splicing factor U2af large subunit A-like [Cucurbita moschata] >XP_022964140.1 splicing factor U2af large subunit A-like [Cucurbita moschata] >XP_022964141.1 splicing factor U2af large subunit A-like [Cucurbita moschata])

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 893/950 (94.00%), Postives = 899/950 (94.63%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITAN ESERHFRHSRGSSLD+QNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANLESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLK DTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKR SRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRRSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RREDRGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAE VG
Sbjct: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAETVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNAS+DSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASIDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM                  LKNIVA
Sbjct: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAK CTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRND DHDDNCPD TYQE
Sbjct: 721 KSMNVAKSCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDTDHDDNCPDDTYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           QGNYPHNGRHKNEVVEDKLCHMGIKDATCFED+VCKSASETIPRGCCDKQSSPGNELHDV
Sbjct: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDVVCKSASETIPRGCCDKQSSPGNELHDV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSE REKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSEKREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYH+RF K
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Cp4.1LG17g02200 vs. NCBI nr
Match: KAG6593827.1 (Splicing factor U2AF 65 kDa subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1693 bits (4384), Expect = 0.0
Identity = 892/950 (93.89%), Postives = 900/950 (94.74%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITANRESERHFRHSRGSSLD+QNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANRESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLK DTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RREDRGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLD QSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG
Sbjct: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPS GALFSLAMGKSLSGVSSNNLATKTNASVDSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSSGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM                  LKNIVA
Sbjct: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAKPCTSC+SAEEEYKSICDVTDVEIKHEIQEISTIVISRND DHDDNCPD TYQE
Sbjct: 721 KSMNVAKPCTSCISAEEEYKSICDVTDVEIKHEIQEISTIVISRNDTDHDDNCPDDTYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           +GNYPHNGRHKNEVVEDKLCHMGIKDATCFED+VCKSASETIP+G CDKQSSPGNELHDV
Sbjct: 781 KGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDVVCKSASETIPQGYCDKQSSPGNELHDV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSE REKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSEKREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYH+RF K
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Cp4.1LG17g02200 vs. NCBI nr
Match: KAG7026156.1 (Splicing factor U2AF 65 kDa subunit [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1681 bits (4353), Expect = 0.0
Identity = 894/978 (91.41%), Postives = 900/978 (92.02%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITANRESERHFRHSRGSSLD+QNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANRESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLK DTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RREDRGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLD QSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG
Sbjct: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPS GALFSLAMGKSLSGVSSNNLATKTNASVDSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSSGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM                  LKNIVA
Sbjct: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLE----------------------------YVDQSVMP 660
           AFGPLKAYHFEINNDLNEPCAFLE                            YVDQSVMP
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLELYINSPKASIYRHIIAHGNPLIISSRLFYVDQSVMP 660

Query: 661 KACAGLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNA 720
           KACAGLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNA
Sbjct: 661 KACAGLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNA 720

Query: 721 DVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKH 780
           DVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKPCTSC+SAEEEYKSICDVTDVEIKH
Sbjct: 721 DVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKPCTSCISAEEEYKSICDVTDVEIKH 780

Query: 781 EIQEISTIVISRNDNDHDDNCPDATYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFED 840
           EIQEISTIVISRND DHDDNCPD TYQEQGNYPHNGRHKNEVVEDKLCHMGIKDAT FED
Sbjct: 781 EIQEISTIVISRNDTDHDDNCPDDTYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATYFED 840

Query: 841 IVCKSASETIPRGCCDKQSSPGNELHDVKVAKIIETGSDEKPVLLGDSTTVVADDEKKVL 900
           +VCKSASETIPRGCCDKQSSPGNELHDVKVAKIIETGSDEKPVLLGDSTTVVADDEKKVL
Sbjct: 841 VVCKSASETIPRGCCDKQSSPGNELHDVKVAKIIETGSDEKPVLLGDSTTVVADDEKKVL 900

Query: 901 HGMDPMVRTDSDTSENREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQ 950
           HGMDPMVRTDSDTSE REKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQ
Sbjct: 901 HGMDPMVRTDSDTSEKREKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQ 938

BLAST of Cp4.1LG17g02200 vs. NCBI nr
Match: XP_023000460.1 (splicing factor U2af large subunit A-like [Cucurbita maxima] >XP_023000461.1 splicing factor U2af large subunit A-like [Cucurbita maxima] >XP_023000462.1 splicing factor U2af large subunit A-like [Cucurbita maxima])

HSP 1 Score: 1672 bits (4330), Expect = 0.0
Identity = 880/950 (92.63%), Postives = 892/950 (93.89%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQ LYNSNDESAARTRPFSFEDIMLRRKNKESAA DGGDT S SR
Sbjct: 1   MSTYSCSKQYSRTSAKQCLYNSNDESAARTRPFSFEDIMLRRKNKESAATDGGDTSSQSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RRE+RGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RRENRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLD QSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSG TRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG
Sbjct: 301 GDFTNSSSSQYRRHSGLTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSL  GKSLSG+SSNNLATKTNAS DSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLTTGKSLSGISSNNLATKTNASFDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DL+KSVAVVNKI+DVVEDSPNKII+AGISNRISSEM                  LKNIVA
Sbjct: 541 DLDKSVAVVNKINDVVEDSPNKIIIAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGG+VLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGEVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAKPCTSCVSAEEEY+SICD+TDVEIKHEIQEISTIVISRND DHDDNC D TYQE
Sbjct: 721 KSMNVAKPCTSCVSAEEEYQSICDITDVEIKHEIQEISTIVISRNDTDHDDNCLDYTYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           QGNYPHNGRHKNEVVEDKLCHMGIKDATCFED+VCKSA ETIPRGCCDKQSSPGNELH V
Sbjct: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDVVCKSALETIPRGCCDKQSSPGNELHGV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDS+TVVADDEKKVLHGMDPMVRTDSDTSE REKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSSTVVADDEKKVLHGMDPMVRTDSDTSEKREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRF K
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFPK 910

BLAST of Cp4.1LG17g02200 vs. ExPASy TrEMBL
Match: A0A6J1HJZ8 (splicing factor U2af large subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC111464254 PE=4 SV=1)

HSP 1 Score: 1694 bits (4388), Expect = 0.0
Identity = 893/950 (94.00%), Postives = 899/950 (94.63%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR
Sbjct: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITAN ESERHFRHSRGSSLD+QNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANLESERHFRHSRGSSLDLQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLK DTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKR SRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKRDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRRSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RREDRGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAE VG
Sbjct: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAETVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNAS+DSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASIDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM                  LKNIVA
Sbjct: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAK CTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRND DHDDNCPD TYQE
Sbjct: 721 KSMNVAKSCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDTDHDDNCPDDTYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           QGNYPHNGRHKNEVVEDKLCHMGIKDATCFED+VCKSASETIPRGCCDKQSSPGNELHDV
Sbjct: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDVVCKSASETIPRGCCDKQSSPGNELHDV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSE REKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSEKREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYH+RF K
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHRRFPK 910

BLAST of Cp4.1LG17g02200 vs. ExPASy TrEMBL
Match: A0A6J1KDP6 (splicing factor U2af large subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC111494723 PE=4 SV=1)

HSP 1 Score: 1672 bits (4330), Expect = 0.0
Identity = 880/950 (92.63%), Postives = 892/950 (93.89%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAANDGGDTGSHSR 60
           MSTYSCSKQYSRTSAKQ LYNSNDESAARTRPFSFEDIMLRRKNKESAA DGGDT S SR
Sbjct: 1   MSTYSCSKQYSRTSAKQCLYNSNDESAARTRPFSFEDIMLRRKNKESAATDGGDTSSQSR 60

Query: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSDR 120
           RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESA+ISSRRKKEETLLKDNMVIRSDR
Sbjct: 61  RESIDKHITANRESERHFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDNMVIRSDR 120

Query: 121 NNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180
           NNY SGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD
Sbjct: 121 NNYESGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGKD 180

Query: 181 RREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHHE 240
           RRE+RGKGKSERESKRKYQHGDDDRNRDRHI+KKHDHGKHHDLESKERKEAKI LSSHHE
Sbjct: 181 RRENRGKGKSERESKRKYQHGDDDRNRDRHITKKHDHGKHHDLESKERKEAKISLSSHHE 240

Query: 241 DSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300
           DSRLKKRRKRSLD QSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI
Sbjct: 241 DSRLKKRRKRSLDRQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDRI 300

Query: 301 GDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360
           GDFTNSSSSQYRRHSG TRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG
Sbjct: 301 GDFTNSSSSQYRRHSGLTRSGLGGYSPRKRRTESAVKTPPVRSPEKKNEGLDLLPAEKVG 360

Query: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSIQL 420
           LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSL  GKSLSG+SSNNLATKTNAS DSIQL
Sbjct: 361 LFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLTTGKSLSGISSNNLATKTNASFDSIQL 420

Query: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480
           TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV
Sbjct: 421 TQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQALV 480

Query: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQTG 540
           EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV                      TG
Sbjct: 481 EFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELV----------------------TG 540

Query: 541 DLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNIVA 600
           DL+KSVAVVNKI+DVVEDSPNKII+AGISNRISSEM                  LKNIVA
Sbjct: 541 DLDKSVAVVNKINDVVEDSPNKIIIAGISNRISSEM------------------LKNIVA 600

Query: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIASLE 660
           AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGG+VLKVFPAVPIASLE
Sbjct: 601 AFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGEVLKVFPAVPIASLE 660

Query: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720
           RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV
Sbjct: 661 RNGCQPCYGIPEHVKPLLQQPTVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTV 720

Query: 721 KSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCPDATYQE 780
           KSMNVAKPCTSCVSAEEEY+SICD+TDVEIKHEIQEISTIVISRND DHDDNC D TYQE
Sbjct: 721 KSMNVAKPCTSCVSAEEEYQSICDITDVEIKHEIQEISTIVISRNDTDHDDNCLDYTYQE 780

Query: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPGNELHDV 840
           QGNYPHNGRHKNEVVEDKLCHMGIKDATCFED+VCKSA ETIPRGCCDKQSSPGNELH V
Sbjct: 781 QGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDVVCKSALETIPRGCCDKQSSPGNELHGV 840

Query: 841 KVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREKKERNNNLES 900
           KVAKIIETGSDEKPVLLGDS+TVVADDEKKVLHGMDPMVRTDSDTSE REKKERNNNLES
Sbjct: 841 KVAKIIETGSDEKPVLLGDSSTVVADDEKKVLHGMDPMVRTDSDTSEKREKKERNNNLES 900

Query: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRF K
Sbjct: 901 SFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFPK 910

BLAST of Cp4.1LG17g02200 vs. ExPASy TrEMBL
Match: A0A6J1C9Y6 (splicing factor U2af large subunit A OS=Momordica charantia OX=3673 GN=LOC111009561 PE=4 SV=1)

HSP 1 Score: 1283 bits (3320), Expect = 0.0
Identity = 715/959 (74.56%), Postives = 795/959 (82.90%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDT--GS 60
           MST+SCSK+YSR+S K +LYN+NDESAARTRPFSFEDIMLRRKNK SAAN +GG T  GS
Sbjct: 1   MSTHSCSKKYSRSSVKHTLYNTNDESAARTRPFSFEDIMLRRKNKGSAANVEGGATVAGS 60

Query: 61  HSRRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIR 120
            SRR+SID  IT NRESER FRHSRGSSLDVQNLPLEESA+ISSRRKKEETLLKD+ V+R
Sbjct: 61  RSRRDSIDNDITDNRESERLFRHSRGSSLDVQNLPLEESAKISSRRKKEETLLKDSTVVR 120

Query: 121 SDRNNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAA 180
           SDRNNY SGLTL+GK+K++TNGKDK QKYGQENLGWGK+D+R+RIDIENE+GKRHSRD A
Sbjct: 121 SDRNNYESGLTLMGKVKYETNGKDKGQKYGQENLGWGKSDQRTRIDIENESGKRHSRDLA 180

Query: 181 GKDRREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSS 240
           GKDRREDRG+GK ERESKRKYQ+GDDD+NRDR+  KKHDH KH DLE+++RKEAK+ LSS
Sbjct: 181 GKDRREDRGRGKFERESKRKYQNGDDDKNRDRYSEKKHDHSKHRDLENRDRKEAKVSLSS 240

Query: 241 HHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDS 300
           H+EDSR K+RRKRS D +SKH++S SLSPR  K STKL R KELP DSH+KKSGRW SDS
Sbjct: 241 HYEDSRFKRRRKRSQDRESKHRRSVSLSPRAHKHSTKLVRHKELP-DSHLKKSGRWRSDS 300

Query: 301 DRIGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPA 360
           DR GDFTNSS+SQYRRHSGST SGLGGYSPRKRRTESA+KTP PVRSPEKKNE LDLLPA
Sbjct: 301 DRTGDFTNSSTSQYRRHSGST-SGLGGYSPRKRRTESAIKTPSPVRSPEKKNEALDLLPA 360

Query: 361 EKVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKT-NASV 420
           +KVGLFSGSVTS FQPSNPTVSS IS D S GALFS AMGKSLSGVSSNNL  KT N S+
Sbjct: 361 DKVGLFSGSVTSIFQPSNPTVSSVISTDQSSGALFSSAMGKSLSGVSSNNLPMKTANVSL 420

Query: 421 DSIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDR 480
           D IQLTQATRPMRRLYIEN+PHS SEKA+I+C+NGFLTSSGVNHIEGTQPCISCIIHKDR
Sbjct: 421 DLIQLTQATRPMRRLYIENVPHSASEKAVIECMNGFLTSSGVNHIEGTQPCISCIIHKDR 480

Query: 481 GQALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAI 540
           GQALVEFLTPEDASAALSFDG++FSGSIL+IRRPKDYVE+V                   
Sbjct: 481 GQALVEFLTPEDASAALSFDGTNFSGSILKIRRPKDYVEIV------------------- 540

Query: 541 FMQTGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQL 600
              TGDL+KS AVVN+ISDVVEDSPNKI +AGISNRISSEM                  L
Sbjct: 541 ---TGDLDKSEAVVNEISDVVEDSPNKIFIAGISNRISSEM------------------L 600

Query: 601 KNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVP 660
           ++IV +FGPLKAYHFEIN DLNEPCAFLEYVDQSVMPKACAGLNGMKIGG+VLKVF AVP
Sbjct: 601 RDIVTSFGPLKAYHFEINGDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGEVLKVFSAVP 660

Query: 661 IASLERNGCQPCYGIPEHVKPLLQQPTVVLKVN-VFNADVLPLLSESDINEVLEDIRFEC 720
            A LERNGCQPCYGIPEHVKPLLQQPTVVLK+N VF ADVLP+LSES+I+EVLEDIRFEC
Sbjct: 661 FAPLERNGCQPCYGIPEHVKPLLQQPTVVLKLNNVFGADVLPVLSESNIDEVLEDIRFEC 720

Query: 721 ARFGTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDNCP 780
           ARFGTVKSMNV KPC S V AEE+YK+     DVEIKH IQ  ST+V SRNDND +D+  
Sbjct: 721 ARFGTVKSMNVVKPCNSFVGAEEKYKN---TNDVEIKHGIQGNSTLVPSRNDNDLEDD-- 780

Query: 781 DATYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQSSPG 840
           +A   ++          NEVVE+KLC MG  DA CFED+ C++  E+IP G   +QSSPG
Sbjct: 781 EANLNDR---------PNEVVENKLCQMGNDDAPCFEDVACENTLESIPGGIPGRQSSPG 840

Query: 841 NELHDVKVAKIIET---GSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREK 900
           NEL D  VA+++ET   GSD K +LL DSTTV AD +KKVL+G+D MVRTDSD  E  EK
Sbjct: 841 NELRDTNVAEMVETDEKGSDNKSMLLDDSTTVGADSKKKVLNGLDAMVRTDSDAFEKVEK 900

Query: 901 KERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
           K+   NL   F +GSVFVEFGR+EASCMAAHSLHGRIYDGQEI +EYIPHDLY KRF K
Sbjct: 901 KDPRTNL---FVLGSVFVEFGRMEASCMAAHSLHGRIYDGQEICIEYIPHDLYRKRFPK 900

BLAST of Cp4.1LG17g02200 vs. ExPASy TrEMBL
Match: A0A6J1H6S0 (splicing factor U2af large subunit B-like OS=Cucurbita moschata OX=3662 GN=LOC111460135 PE=4 SV=1)

HSP 1 Score: 1214 bits (3140), Expect = 0.0
Identity = 679/959 (70.80%), Postives = 761/959 (79.35%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60
           MS+YS SK YSR S KQ+L NSNDESAARTRP SFEDIMLRRKNK SA   + G TGSHS
Sbjct: 1   MSSYSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVGVTGSHS 60

Query: 61  RRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSD 120
           RRESIDK  T N  SERHFRH +G+SLDVQNL LEES + SSRRKKEETLLKDNM +RSD
Sbjct: 61  RRESIDKLSTDNCASERHFRHGKGASLDVQNLSLEESGKDSSRRKKEETLLKDNMEVRSD 120

Query: 121 RNNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180
           RNNY S LTL+GKLK+D NG DK+QKYGQEN+G GK ++ SR+DIE ETGKRHSRD+  K
Sbjct: 121 RNNYDSELTLMGKLKYDANGNDKKQKYGQENVGRGKKNQSSRVDIEKETGKRHSRDSKAK 180

Query: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHH 240
             RED G+G  ER  KRK Q+GDD+ NRD++ +K+HDHGKHHD E++ERKEAK  L+S++
Sbjct: 181 --REDLGRGNFERGGKRKDQNGDDEGNRDKYAAKRHDHGKHHDPENRERKEAKASLTSNY 240

Query: 241 EDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300
           EDSRL++RRKRS D +SKH++S SLSPRP K S+KL RQKELPLDSHVKKSGRW SDSDR
Sbjct: 241 EDSRLQRRRKRSQDRESKHRRSVSLSPRPHKHSSKLVRQKELPLDSHVKKSGRWRSDSDR 300

Query: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360
            GD TNSS+SQYRRH GST SGLGGYSPRKRRTESA+KTP PV+SPEKK+EGLD+ P EK
Sbjct: 301 TGDLTNSSNSQYRRHFGST-SGLGGYSPRKRRTESAIKTPSPVQSPEKKDEGLDVPPTEK 360

Query: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSI 420
           +GLFS S+ SNFQPSN TVSSGI N  SGGA+FS  +GKSL+GVSSNN+  KTN S+D I
Sbjct: 361 IGLFSSSINSNFQPSNATVSSGIINVQSGGAIFSSVIGKSLTGVSSNNIEMKTNVSLDLI 420

Query: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480
           QLTQATRPMRRLYIENLPHS SEKAIIDCLNGFLTSSGVNHI+GT PCISCIIHKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGVNHIKGTLPCISCIIHKDRGQA 480

Query: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQ 540
           LVEFLTPEDASAALSFDGSDFSGSIL+IRRPKDYVE+V                      
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV---------------------- 540

Query: 541 TGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNI 600
           TG L+KSVAVVNKI D VEDSPNKI +AGISNRISSEM                  L++I
Sbjct: 541 TGGLDKSVAVVNKIIDTVEDSPNKIFIAGISNRISSEM------------------LRDI 600

Query: 601 VAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIAS 660
           V AFGPLKA+HFE+N+DLNE CAFLEYVDQS++ KACAGLNGMKIGG+VLKVFPAVP  S
Sbjct: 601 VTAFGPLKAFHFEMNDDLNERCAFLEYVDQSIVSKACAGLNGMKIGGEVLKVFPAVPFTS 660

Query: 661 LERNGCQPCYGIPEHVKPLLQQPTVVLKVN-VFNADVLPLLSESDINEVLEDIRFECARF 720
           LERN CQP YGIPEHVKPLLQQPTVVLKVN VFNADVLP+LSE+DI+EVLEDIR ECARF
Sbjct: 661 LERNECQPWYGIPEHVKPLLQQPTVVLKVNNVFNADVLPVLSEADIDEVLEDIRVECARF 720

Query: 721 GTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDN----- 780
           GTVKSMN  KPC  C SAEE+YK+I D+TDVEIKHEIQE  T+ ISRN ND +DN     
Sbjct: 721 GTVKSMNFVKPCNGCFSAEEDYKNISDITDVEIKHEIQENCTMAISRNGNDVEDNNVNLD 780

Query: 781 -CPDATYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQS 840
            CP+ T Q QGN P NGRH++EV E KLC MG  DAT FE + C++ASE IPRG  ++QS
Sbjct: 781 NCPNDTNQRQGNCPGNGRHQDEV-EVKLCRMGQDDATRFEIVACENASERIPRGLSEQQS 840

Query: 841 SPGNELHDVKVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENREK 900
           SPGN+  D KVA+ IET               ++ D+K V      MVRTD DTSE  EK
Sbjct: 841 SPGNQHQDAKVAETIETNE-------------ISPDKKSVCIDDSAMVRTDFDTSEKSEK 900

Query: 901 KERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRFSK 950
            +  NNL S F +GSVFVEFGR EASCMAAHSLHGRIYDGQEIS+EYIPHDLY KRF K
Sbjct: 901 DDPRNNLASLFVLGSVFVEFGRTEASCMAAHSLHGRIYDGQEISIEYIPHDLYRKRFPK 902

BLAST of Cp4.1LG17g02200 vs. ExPASy TrEMBL
Match: A0A6J1L124 (splicing factor U2af large subunit B-like OS=Cucurbita maxima OX=3661 GN=LOC111498921 PE=4 SV=1)

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 684/962 (71.10%), Postives = 760/962 (79.00%), Query Frame = 0

Query: 1   MSTYSCSKQYSRTSAKQSLYNSNDESAARTRPFSFEDIMLRRKNKESAAN-DGGDTGSHS 60
           MS++S SK YSR S KQ+L NSNDESAARTRP SFEDIMLRRKNK SA   + G TGSHS
Sbjct: 1   MSSFSFSKHYSRRSKKQTLSNSNDESAARTRPLSFEDIMLRRKNKGSADTVEVGVTGSHS 60

Query: 61  RRESIDKHITANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEETLLKDNMVIRSD 120
           RRESIDK  T N  SERHFRH +G+S DVQNL LEES + SSRRKKEETLLKDNM  RSD
Sbjct: 61  RRESIDKLSTDNCASERHFRHGKGASPDVQNLSLEESGKDSSRRKKEETLLKDNMEFRSD 120

Query: 121 RNNYGSGLTLIGKLKHDTNGKDKRQKYGQENLGWGKNDERSRIDIENETGKRHSRDAAGK 180
           RNNY S LTL+GKLK+D NG DK+QKYG EN+G GKN++ SR+DIE ETGKRHSRD+  K
Sbjct: 121 RNNYDSELTLMGKLKYDANGNDKKQKYGLENVGRGKNNQSSRVDIEKETGKRHSRDS--K 180

Query: 181 DRREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLSSHH 240
            RRED G+GK ER  KRK Q+GDD+ NRD++ +K+HDHGKHHD E++ERKEAK  L+S++
Sbjct: 181 ARREDLGRGKFERGGKRKDQNGDDEGNRDKYAAKRHDHGKHHDPENRERKEAKGSLTSNY 240

Query: 241 EDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSDSDR 300
           EDSRLKKRRKRS D +SKH+    +SPRP K STKLARQKELPLDS+VKKSGRW SDSDR
Sbjct: 241 EDSRLKKRRKRSQDRESKHR----ISPRPHKHSTKLARQKELPLDSNVKKSGRWRSDSDR 300

Query: 301 IGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTP-PVRSPEKKNEGLDLLPAEK 360
            GD TNSS+SQYRR SGST SGLGGYSPRKRRTESAVKTP PV+SPEKK+EGLDL P EK
Sbjct: 301 TGDLTNSSNSQYRRQSGST-SGLGGYSPRKRRTESAVKTPSPVQSPEKKDEGLDLPPTEK 360

Query: 361 VGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNASVDSI 420
           +GLFS S+ SNFQPSN TVSSGI N  SGGA+FS  +G+SLSGVSSNN+  KTN S+D I
Sbjct: 361 IGLFSSSINSNFQPSNTTVSSGIINVQSGGAIFSSVIGRSLSGVSSNNIEMKTNVSLDLI 420

Query: 421 QLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKDRGQA 480
           QLTQATRPMRRLYIENLPHS SEKAIIDCLNGFLTSSG+NHI+GT PCISCIIHKDRGQA
Sbjct: 421 QLTQATRPMRRLYIENLPHSASEKAIIDCLNGFLTSSGINHIKGTLPCISCIIHKDRGQA 480

Query: 481 LVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNRGGFQTVAIFMQ 540
           LVEFLTPEDASAALSFDGSDFSGSIL+IRRPKDYVE+V                      
Sbjct: 481 LVEFLTPEDASAALSFDGSDFSGSILKIRRPKDYVEIV---------------------- 540

Query: 541 TGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFSLCCVHSGDYFFNVILQLKNI 600
           TG L+KSVAVVNKI D VEDSPNKI +AGISNRISSEM                  L++I
Sbjct: 541 TGGLDKSVAVVNKIIDTVEDSPNKIFIAGISNRISSEM------------------LRDI 600

Query: 601 VAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAVPIAS 660
           V AFGPLKAYHFE+N+DLNE CAFLEYVDQS++ KACAGLNGMKIGG+VLKVFPAVP   
Sbjct: 601 VTAFGPLKAYHFEMNDDLNERCAFLEYVDQSIVSKACAGLNGMKIGGEVLKVFPAVPFTP 660

Query: 661 LERNGCQPCYGIPEHVKPLLQQPTVVLKVN-VFNADVLPLLSESDINEVLEDIRFECARF 720
           LERN CQP YGIPEHVKPLLQQPTVVLKVN VFNADVLP+LSE+DI+EVLEDIR ECARF
Sbjct: 661 LERNECQPWYGIPEHVKPLLQQPTVVLKVNNVFNADVLPVLSEADIDEVLEDIRVECARF 720

Query: 721 GTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDN----- 780
           GTVKSMN  KPC  C SAEE+YK+I D+TDVEIKHEIQE  T+ ISRN ND +DN     
Sbjct: 721 GTVKSMNFVKPCNGCFSAEEDYKNISDITDVEIKHEIQENCTMAISRNGNDVEDNNVNLD 780

Query: 781 -CPDATYQEQGNYPHNGRHKNEVVEDKLCHMGIKDATCFEDIVCKSASETIPRGCCDKQS 840
            CP+ T Q QGN P NGRH +EVVEDKLC MG  DAT FE + C++ASE  PRG  ++QS
Sbjct: 781 NCPNGTNQRQGNCPGNGRHHDEVVEDKLCRMGQDDATRFEIVACENASERNPRGLSEQQS 840

Query: 841 SPGNELHDVKVAKIIETGS---DEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSEN 900
           SPGN+  D KVA+ IET     D+K V L DS                 MVRTDSDTS  
Sbjct: 841 SPGNQHRDAKVAETIETNEISPDKKSVCLNDSA----------------MVRTDSDTSAK 899

Query: 901 REKKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRF 950
            +K +R NNL S F +GSVFVEFGR EASCMAAHSLHGRIYDGQEIS+EYIPHDLY KRF
Sbjct: 901 SKKGDRRNNLASLFVLGSVFVEFGRTEASCMAAHSLHGRIYDGQEISIEYIPHDLYRKRF 899

BLAST of Cp4.1LG17g02200 vs. TAIR 10
Match: AT2G33435.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 215.3 bits (547), Expect = 2.1e-55
Identity = 172/473 (36.36%), Postives = 256/473 (54.12%), Query Frame = 0

Query: 70  ANRESERHFRHSRGSSLDVQNLPLEESARISSRRKKEE---TLLKDNMVIRSDRNNYGSG 129
           ++R+ E     SR  +LD Q    ++    S+R++K E   +  +   +    ++  G  
Sbjct: 521 SSRKKEEAISSSREENLDKQ----KKDESTSNRKRKAEGECSTAETESIEEHSKDRRGK- 580

Query: 130 LTLIGKLKHDTNGKDKR--QKYGQENLGWGKNDE--------RSRIDIENETGKRHSRDA 189
                K + ++N +++R  +K  +E+L   +  E        ++  D +    K+  R  
Sbjct: 581 -----KEETNSNCREERRDKKMKKEDLASNRKIEGEIPTTETKTMTDRDGLCSKKRLRSL 640

Query: 190 AGKDRREDRGKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHHDLESKERKEAKILLS 249
              D   D    K +   KRK Q+G+  +NR+ ++SK+HD GK H +E  ER E +    
Sbjct: 641 VVADVPRDESSIKPDNGDKRKNQNGNHKKNREINMSKRHDPGKVHSVEVSERWERREQPK 700

Query: 250 SHHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELPLDSHVKKSGRWHSD 309
           S   D R K+RR RS DH    +K  S  PR  K++++  R  E   ++ VK     H  
Sbjct: 701 SRQRDLREKRRRSRSRDHGQDRQKRSSPLPRAEKATSRHKRNHEERSENVVKDRSGKHHC 760

Query: 310 SDRIGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVK--TPPVRSPEKKNEGLDLL 369
           +D     T++ S++ RR+S S +S LGGYSPRKRR +++ K  +PP  S EKK+    L 
Sbjct: 761 NDNEDKVTSTVSNKSRRYSAS-KSELGGYSPRKRREQASTKAASPPNLSSEKKSAKWGLA 820

Query: 370 PAEKVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKSLSGVSSNNLATKTNAS 429
                G+FS SV S  Q +  T    IS      A  +L     +          +   S
Sbjct: 821 ATVTAGMFSDSVFSGLQAATQTAYPTISE-----ASLTLLKPLMVMDAPFRTPPARQTTS 880

Query: 430 VDSIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVNHIEGTQPCISCIIHKD 489
            DS+QLT++TR MRRLY EN+P S SEK++I+C NG++ SSG NHI+G++PCISCII+K+
Sbjct: 881 FDSVQLTESTRRMRRLYAENVPDSASEKSLIECFNGYMLSSGSNHIKGSEPCISCIINKE 940

Query: 490 RGQALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMKLSYIFFSNR 528
           + QALVEFLTP+DASAALS DG  F+GS L+IRRPKDYV   +    + F N+
Sbjct: 941 KSQALVEFLTPQDASAALSLDGCSFAGSNLKIRRPKDYVRTTVSSIMVVFYNQ 977

BLAST of Cp4.1LG17g02200 vs. TAIR 10
Match: AT2G33440.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 191.8 bits (486), Expect = 2.5e-48
Identity = 125/358 (34.92%), Postives = 187/358 (52.23%), Query Frame = 0

Query: 595 LKNIVAAFGPLKAYHFEINNDLNEPCAFLEYVDQSVMPKACAGLNGMKIGGQVLKVFPAV 654
           L  IV+ FGPLKAY F  NNDLN+ CAFLEY D SV  KACAGLNGM++GG V+    A 
Sbjct: 2   LMEIVSVFGPLKAYRFVSNNDLNQRCAFLEYTDGSVTLKACAGLNGMRLGGSVITAVCAF 61

Query: 655 PIA-SLERNGCQPCYGIPEHVKPLLQQPTVVLKV-NVFNADVLPLLSESDINEVLEDIRF 714
           P A S+  N   P YGIP H KPLL +P  +LK+ NV + + L   SE ++ E+LED+R 
Sbjct: 62  PDASSVAVNENPPFYGIPSHAKPLLGKPKNILKLKNVVDPEDLTSFSEQEVKEILEDVRL 121

Query: 715 ECARFGTVKSMNVAKPCTSCVSAEEEYKSICDVTDVEIKHEIQEISTIVISRNDNDHDDN 774
           ECARFG +KS+N+ +  +  ++  E    + ++   + K    E++  VI   D      
Sbjct: 122 ECARFGVIKSINILEHKSKDITVSET-NPLLNLESTDSK----EMNVSVIEEKD------ 181

Query: 775 CPDATYQEQGNYPHNGRHKNEVVEDK--LCHMGIKDATCFEDIVCKSASETIPRGCCDKQ 834
                          G  K E + D   L  + + D+   ED +C+  S+T      +  
Sbjct: 182 --------------EGSEKAEDIADNVDLAEVVMPDSLTGEDKLCEPCSDT----AAETS 241

Query: 835 SSPGNELHDVKVAKIIETGSDEKPVLLGDSTTVVADDEKKVLHGMDPMVRTDSDTSENRE 894
           +    +LH         T  +    ++ +S    A++ ++V        R D+    ++ 
Sbjct: 242 TQENEDLH--------STEQEHCEKIVEESAQAEAENPQEVASVRTVKTRWDAG---DKI 301

Query: 895 KKERNNNLESSFAVGSVFVEFGRLEASCMAAHSLHGRIYDGQEISVEYIPHDLYHKRF 949
           ++E+  + E  F  G +F+E+ R EA+C AAHSLHGR+YD + +  EY+  +LY  RF
Sbjct: 302 EEEQEEDPEDVFETGCIFIEYRRPEATCDAAHSLHGRLYDNRIVKAEYVSKELYQIRF 319

BLAST of Cp4.1LG17g02200 vs. TAIR 10
Match: AT4G36690.4 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 94.4 bits (233), Expect = 5.4e-19
Identity = 128/534 (23.97%), Postives = 216/534 (40.45%), Query Frame = 0

Query: 223 LESKERKEAKILLSSHHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKELP 282
           L+SK ++E++     H  ++   K R+R        ++   +S R               
Sbjct: 33  LDSKPKRESR----DHERETSRSKDREREKGRDKDRERDSEVSRR--------------S 92

Query: 283 LDSHVKKSGRWHSDSDRIGDFTNSSSSQYRRHS---GSTRSGLGGYSPRKRRTES---AV 342
            D   +KS     D DR     +  SS++R HS   G  R   GG   R+ R+ S   + 
Sbjct: 93  RDRDGEKSKERSRDKDRDHRERHHRSSRHRDHSRERGERRERGGGRRSRRSRSRSKDRSE 152

Query: 343 KTPPVRSPEKKNE---GLDLLPAEKVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSL 402
           +    RSP K  +   G D+ P     L +G+  +   P  P    G    P+   +F L
Sbjct: 153 RRTRSRSPSKSKQRVSGFDMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPL 212

Query: 403 AMGKSLSGVSSNNLATKTNASVDSIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLT 462
             G+S  G+S   +   T          QATR  RR+Y+  L  + +E+++    +  + 
Sbjct: 213 PTGQSFGGLSMMPIQAMTQ---------QATRHARRVYVGGLSPTANEQSVATFFSQVMA 272

Query: 463 SSGVNHIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYV 522
           + G N        ++  I+ ++  A VE  + E+AS A+S DG  F G+ +++RRP DY 
Sbjct: 273 AVGGNTAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY- 332

Query: 523 ELVMKLSYIFFSNRGGFQTVAIFMQTGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRIS 582
                       N     T+     +  L  +   +   +    + P++I V G+     
Sbjct: 333 ------------NPSLAATLGPSQPSPHLNLAAVGLTPGASGGLEGPDRIFVGGL----- 392

Query: 583 SEMFSLCCVHSGDYFFNVILQLKNIVAAFGPLKAYHFEINNDL--NEPCAFLEYVDQSVM 642
                        Y+F    Q++ ++ +FG LK +    + +   ++  AF  Y D SV 
Sbjct: 393 ------------PYYFTE-SQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVT 452

Query: 643 PKACAGLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQ------------ 702
             ACA LNG+K+G + L V  A     L++         PE    LL             
Sbjct: 453 DIACAALNGIKMGDKTLTVRRANQGTMLQK---------PEQENVLLHAQQQIAFQRVML 494

Query: 703 QP-----TVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKP 729
           QP     TVV    V   D   L  + +  +++ED+R E  +FG + ++ + +P
Sbjct: 513 QPGAVATTVVCLTQVVTED--ELRDDEEYGDIMEDMRQEGGKFGALTNVVIPRP 494

BLAST of Cp4.1LG17g02200 vs. TAIR 10
Match: AT4G36690.1 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 82.0 bits (201), Expect = 2.8e-15
Identity = 139/589 (23.60%), Postives = 237/589 (40.24%), Query Frame = 0

Query: 163 DIENETGKRHSRDAAGKDRREDR-GKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHH 222
           + E+  G     DA   +    R G+ + + +SK K +  D +R   R   ++ + G+  
Sbjct: 3   EFEDHEGNGTVADAIYDEENGGRDGEIEDQLDSKPKRESRDHERETSRSKDREREKGRDK 62

Query: 223 DLESKERKEAKILLSSHHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKEL 282
           D E    +++++   S   D   +K ++RS D    H++    S R    S +   ++E 
Sbjct: 63  DRE----RDSEVSRRSRDRDG--EKSKERSRDKDRDHRERHHRSSRHRDHSRERGERRE- 122

Query: 283 PLDSHVKKSGRWHSDSDRIGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPV 342
                  + GR   D  R  D  +      R    S RS        +RRT S       
Sbjct: 123 -------RGGRDDDDYRRSRDRDHDRRRDDRGGRRSRRSRSRSKDRSERRTRS------- 182

Query: 343 RSPEKKNE---GLDLLPAEKVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKS 402
           RSP K  +   G D+ P     L +G+  +   P  P    G    P+   +F L  G+S
Sbjct: 183 RSPSKSKQRVSGFDMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQS 242

Query: 403 LSGVSSNNLATKTNASVDSIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVN 462
             G+S   +   T          QATR  RR+Y+  L  + +E+++    +  + + G N
Sbjct: 243 FGGLSMMPIQAMTQ---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGN 302

Query: 463 HIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMK 522
                   ++  I+ ++  A VE  + E+AS A+S DG  F G+ +++RRP DY      
Sbjct: 303 TAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY------ 362

Query: 523 LSYIFFSNRGGFQTVAIFMQTGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFS 582
                  N     T+     +  L  +   +   +    + P++I V G+          
Sbjct: 363 -------NPSLAATLGPSQPSPHLNLAAVGLTPGASGGLEGPDRIFVGGL---------- 422

Query: 583 LCCVHSGDYFFNVILQLKNIVAAFGPLKAYHFEINNDL--NEPCAFLEYVDQSVMPKACA 642
                   Y+F    Q++ ++ +FG LK +    + +   ++  AF  Y D SV   ACA
Sbjct: 423 -------PYYFTE-SQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVTDIACA 482

Query: 643 GLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQ------------QP--- 702
            LNG+K+G + L V  A     L++         PE    LL             QP   
Sbjct: 483 ALNGIKMGDKTLTVRRANQGTMLQK---------PEQENVLLHAQQQIAFQRVMLQPGAV 516

Query: 703 --TVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFGTVKSMNVAKP 729
             TVV    V   D   L  + +  +++ED+R E  +FG + ++ + +P
Sbjct: 543 ATTVVCLTQVVTED--ELRDDEEYGDIMEDMRQEGGKFGALTNVVIPRP 516

BLAST of Cp4.1LG17g02200 vs. TAIR 10
Match: AT4G36690.2 (U2 snRNP auxilliary factor, large subunit, splicing factor )

HSP 1 Score: 78.2 bits (191), Expect = 4.0e-14
Identity = 138/579 (23.83%), Postives = 231/579 (39.90%), Query Frame = 0

Query: 163 DIENETGKRHSRDAAGKDRREDR-GKGKSERESKRKYQHGDDDRNRDRHISKKHDHGKHH 222
           + E+  G     DA   +    R G+ + + +SK K +  D +R   R   ++ + G+  
Sbjct: 3   EFEDHEGNGTVADAIYDEENGGRDGEIEDQLDSKPKRESRDHERETSRSKDREREKGRDK 62

Query: 223 DLESKERKEAKILLSSHHEDSRLKKRRKRSLDHQSKHKKSGSLSPRPPKSSTKLARQKEL 282
           D E    +++++   S   D   +K ++RS D    H++    S R    S +   ++E 
Sbjct: 63  DRE----RDSEVSRRSRDRDG--EKSKERSRDKDRDHRERHHRSSRHRDHSRERGERRE- 122

Query: 283 PLDSHVKKSGRWHSDSDRIGDFTNSSSSQYRRHSGSTRSGLGGYSPRKRRTESAVKTPPV 342
                  + GR   D  R  D  +      R    S RS        +RRT S       
Sbjct: 123 -------RGGRDDDDYRRSRDRDHDRRRDDRGGRRSRRSRSRSKDRSERRTRS------- 182

Query: 343 RSPEKKNE---GLDLLPAEKVGLFSGSVTSNFQPSNPTVSSGISNDPSGGALFSLAMGKS 402
           RSP K  +   G D+ P     L +G+  +   P  P    G    P+   +F L  G+S
Sbjct: 183 RSPSKSKQRVSGFDMAPPASAMLAAGAAVTGQVPPAPPTLPGAGMFPN---MFPLPTGQS 242

Query: 403 LSGVSSNNLATKTNASVDSIQLTQATRPMRRLYIENLPHSPSEKAIIDCLNGFLTSSGVN 462
             G+S   +   T          QATR  RR+Y+  L  + +E+++    +  + + G N
Sbjct: 243 FGGLSMMPIQAMTQ---------QATRHARRVYVGGLSPTANEQSVATFFSQVMAAVGGN 302

Query: 463 HIEGTQPCISCIIHKDRGQALVEFLTPEDASAALSFDGSDFSGSILRIRRPKDYVELVMK 522
                   ++  I+ ++  A VE  + E+AS A+S DG  F G+ +++RRP DY      
Sbjct: 303 TAGPGDAVVNVYINHEKKFAFVEMRSVEEASNAMSLDGIIFEGAPVKVRRPSDY------ 362

Query: 523 LSYIFFSNRGGFQTVAIFMQTGDLEKSVAVVNKISDVVEDSPNKIIVAGISNRISSEMFS 582
                  N     T+     +  L  +   +   +    + P++I V G+          
Sbjct: 363 -------NPSLAATLGPSQPSPHLNLAAVGLTPGASGGLEGPDRIFVGGL---------- 422

Query: 583 LCCVHSGDYFFNVILQLKNIVAAFGPLKAYHFEINNDL--NEPCAFLEYVDQSVMPKACA 642
                   Y+F    Q++ ++ +FG LK +    + +   ++  AF  Y D SV   ACA
Sbjct: 423 -------PYYFTE-SQVRELLESFGGLKGFDLVKDRETGNSKGYAFCVYQDLSVTDIACA 482

Query: 643 GLNGMKIGGQVLKVFPAVPIASLERNGCQPCYGIPEHVKPLLQ------------QP--- 702
            LNG+K+G + L V  A     L++         PE    LL             QP   
Sbjct: 483 ALNGIKMGDKTLTVRRANQGTMLQK---------PEQENVLLHAQQQIAFQRVMLQPGAV 506

Query: 703 --TVVLKVNVFNADVLPLLSESDINEVLEDIRFECARFG 719
             TVV    V   D   L  + +  +++ED+R E  +FG
Sbjct: 543 ATTVVCLTQVVTED--ELRDDEEYGDIMEDMRQEGGKFG 506