Cp4.1LG16g08430 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG16g08430
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG16: 8054835 .. 8055950 (+)
RNA-Seq Expression	Cp4.1LG16g08430
Synteny	Cp4.1LG16g08430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGCACCCATCTCCTGTGCTACAACAGAGGTTCCTGCAGAAAAGTGGCGATTAGAAGCTTGGTTTTCGGAACATGTGAAGCCCTTAGCGGCGCGCAAGGCGGATCTTGATCCAGCCCTTGTGGCAGCTGAAGAAAGCTCCACCGTCGTGAAAGTAAGGGCCGACGGAAGTGGAGATTTCAAGACTATCACCAAAGCCATTGCCAGCGTTCCTGTTGGTAACACCAAGCGCGTCGTGATTTGGATTGGAGGAGGGGTTTATAAAGAGAAGGTTAGGATCGATAGGAATAAGCCATTTATTACCCTTTATGGCTCTCCTAACAATATGCCAAACTTGACATTTGATGGAGACGCTACAAAATACGGCACCATTTACAGCCCAACTTTGATAGTTGAGGCTGATTATTTCGTTGCTGCAAATCTGGTGATTGAGGTAATTTGTTAATTAAATGCGTTTTGGGTTAATTGGGTGTCGTTTTTTGATAATTTTTTTTTAAATTAATACAATCAGAATTCATCTCCAAGGCCGGAAGGGATAAAAGGAGGTCAGGCATTAGCGGCAAGAATGAGAGGTAATAAAGCTGCAATTTACAATTGCAAGTTCATAGGATTTCAAGACACCCTGTGCGACGATGACGGGTTGCATTTTTACAAAGATTGCTTCGTCCAGGGCACAGTGGACTTCATCTTCGGGAAGGGAACGTCCCTTCATTTGAACACTCAGATGAACGTGGTGGGAATCGGGGGATTGGGAGTGGTGGCAGCGCATTCAAGGGAAAAGGAAGATGACCCGAGTGGGTATGTGTTCGCGCATTGCAGCATCACAGGAACAGGGGGGAAAAACACTTATCTGGGAAGATCATGGAGGCCATGGTCTAGAGTTGTGTTCGCCTACACCAGCATTGCGGACATCCTTCATCCTGAAGGCTGGGACGACATGAACAACCCCGCTTATGACCATACGGTTTTCTACGGAGAATACAAGTGTTCGGGGCTGGGAGCGGTATCCACCGGCCGTGCCAAATTCGGGAAGCAGCTCACTAAAGCACAAGTGAAACCCTTCATCAACCTTGATTTCGTGCACGCCCACAAGTGGTTGCTTCCTCCTCCCAAACTA

mRNA sequence

TTTGCACCCATCTCCTGTGCTACAACAGAGGTTCCTGCAGAAAAGTGGCGATTAGAAGCTTGGTTTTCGGAACATGTGAAGCCCTTAGCGGCGCGCAAGGCGGATCTTGATCCAGCCCTTGTGGCAGCTGAAGAAAGCTCCACCGTCGTGAAAGTAAGGGCCGACGGAAGTGGAGATTTCAAGACTATCACCAAAGCCATTGCCAGCGTTCCTGTTGGTAACACCAAGCGCGTCGTGATTTGGATTGGAGGAGGGGTTTATAAAGAGAAGGTTAGGATCGATAGGAATAAGCCATTTATTACCCTTTATGGCTCTCCTAACAATATGCCAAACTTGACATTTGATGGAGACGCTACAAAATACGGCACCATTTACAGCCCAACTTTGATAGTTGAGGCTGATTATTTCGTTGCTGCAAATCTGGTGATTGAGAATTCATCTCCAAGGCCGGAAGGGATAAAAGGAGGTCAGGCATTAGCGGCAAGAATGAGAGGTAATAAAGCTGCAATTTACAATTGCAAGTTCATAGGATTTCAAGACACCCTGTGCGACGATGACGGGTTGCATTTTTACAAAGATTGCTTCGTCCAGGGCACAGTGGACTTCATCTTCGGGAAGGGAACGTCCCTTCATTTGAACACTCAGATGAACGTGGTGGGAATCGGGGGATTGGGAGTGGTGGCAGCGCATTCAAGGGAAAAGGAAGATGACCCGAGTGGGTATGTGTTCGCGCATTGCAGCATCACAGGAACAGGGGGGAAAAACACTTATCTGGGAAGATCATGGAGGCCATGGTCTAGAGTTGTGTTCGCCTACACCAGCATTGCGGACATCCTTCATCCTGAAGGCTGGGACGACATGAACAACCCCGCTTATGACCATACGGTTTTCTACGGAGAATACAAGTGTTCGGGGCTGGGAGCGGTATCCACCGGCCGTGCCAAATTCGGGAAGCAGCTCACTAAAGCACAAGTGAAACCCTTCATCAACCTTGATTTCGTGCACGCCCACAAGTGGTTGCTTCCTCCTCCCAAACTA

Coding sequence (CDS)

TTTGCACCCATCTCCTGTGCTACAACAGAGGTTCCTGCAGAAAAGTGGCGATTAGAAGCTTGGTTTTCGGAACATGTGAAGCCCTTAGCGGCGCGCAAGGCGGATCTTGATCCAGCCCTTGTGGCAGCTGAAGAAAGCTCCACCGTCGTGAAAGTAAGGGCCGACGGAAGTGGAGATTTCAAGACTATCACCAAAGCCATTGCCAGCGTTCCTGTTGGTAACACCAAGCGCGTCGTGATTTGGATTGGAGGAGGGGTTTATAAAGAGAAGGTTAGGATCGATAGGAATAAGCCATTTATTACCCTTTATGGCTCTCCTAACAATATGCCAAACTTGACATTTGATGGAGACGCTACAAAATACGGCACCATTTACAGCCCAACTTTGATAGTTGAGGCTGATTATTTCGTTGCTGCAAATCTGGTGATTGAGAATTCATCTCCAAGGCCGGAAGGGATAAAAGGAGGTCAGGCATTAGCGGCAAGAATGAGAGGTAATAAAGCTGCAATTTACAATTGCAAGTTCATAGGATTTCAAGACACCCTGTGCGACGATGACGGGTTGCATTTTTACAAAGATTGCTTCGTCCAGGGCACAGTGGACTTCATCTTCGGGAAGGGAACGTCCCTTCATTTGAACACTCAGATGAACGTGGTGGGAATCGGGGGATTGGGAGTGGTGGCAGCGCATTCAAGGGAAAAGGAAGATGACCCGAGTGGGTATGTGTTCGCGCATTGCAGCATCACAGGAACAGGGGGGAAAAACACTTATCTGGGAAGATCATGGAGGCCATGGTCTAGAGTTGTGTTCGCCTACACCAGCATTGCGGACATCCTTCATCCTGAAGGCTGGGACGACATGAACAACCCCGCTTATGACCATACGGTTTTCTACGGAGAATACAAGTGTTCGGGGCTGGGAGCGGTATCCACCGGCCGTGCCAAATTCGGGAAGCAGCTCACTAAAGCACAAGTGAAACCCTTCATCAACCTTGATTTCGTGCACGCCCACAAGTGGTTGCTTCCTCCTCCCAAACTA

Protein sequence

FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL

Homology

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BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 386.0 bits (990), Expect = 4.6e-106
Identity = 183/339 (53.98%), Postives = 242/339 (71.39%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +P  K ++  WF+ HV PLA RK  LDPALVAAE +  ++ V   G G+FKT+T AI 
Sbjct: 27  TPIPEGKPQVVQWFNTHVGPLAQRKG-LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 86

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           SVP GNTKRV+I +  G Y+EKV IDRNKPFITL G PN MP +T+DG A KYGT+ S +
Sbjct: 87  SVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSAS 146

Query: 129 LIVEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 188
           LI+ +DYF+A N+V++N++P P+G  KG QAL+ R+ GN AA YNCKF GFQDT+CDD G
Sbjct: 147 LIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTG 206

Query: 189 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 248
            HF+KDC+V+GT DFIFG GTS++L TQ++VVG  G+ V+AAH+ +  ++ SGY F HC 
Sbjct: 207 NHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG-DGIRVIAAHAGKSAEEKSGYSFVHCK 266

Query: 249 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 308
           +TGTGG   YLGR+W    +VV+AYT +  +++P GW +   PA+D TVFYGEYKCSG G
Sbjct: 267 VTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPG 326

Query: 309 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 347
           +    R  F + +   +   F++L ++   KWLLPPP L
Sbjct: 327 SHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 7.9e-106
Identity = 183/339 (53.98%), Postives = 242/339 (71.39%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +P  K ++  WF+ +V PLA RK  LDPALVAAE +  ++ V   G G+FKT+T AI 
Sbjct: 27  TPIPEGKPQVAQWFNANVGPLAQRKG-LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 86

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           SVP GNTKRV+I +  G YKEKV IDRNKPFITL G PN MP +T+DG A KYGT+ S +
Sbjct: 87  SVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSAS 146

Query: 129 LIVEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 188
           LI+ +DYF+A N+V++N++P P+G  KG QAL+ R+ GN AA YNCKF GFQDT+CDD G
Sbjct: 147 LIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTG 206

Query: 189 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 248
            HF+KDC+V+GT DFIFG GTS++L TQ++VVG  G+ V+AAH+ +  ++ SGY F HC 
Sbjct: 207 NHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG-DGIRVIAAHAGKSAEEKSGYSFVHCK 266

Query: 249 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 308
           +TGTGG   YLGR+W    +VV+AYT +  +++P GW +   PA+D TVFYGEYKCSG G
Sbjct: 267 VTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPG 326

Query: 309 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 347
           +    R  F + +   +   F++L ++   KWLLPPP L
Sbjct: 327 SHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 355.5 bits (911), Expect = 6.7e-97
Identity = 171/335 (51.04%), Postives = 223/335 (66.57%), Query Frame = 0


Query: 11  VPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIASV 70
           +P  K R+E WF+ +VK                           +G G FKTIT+AI SV
Sbjct: 30  IPENKGRIEQWFNTNVK--------------------------QNGRGHFKTITEAINSV 89

Query: 71  PVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPTLI 130
             GNT+RV+I IG GVYKEKV IDR+KPFITLYG PN MP LTFDG A +YGT+ S TLI
Sbjct: 90  RAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPVLTFDGTAAQYGTVDSATLI 149

Query: 131 VEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDGLH 190
           V +DYF+A N++++NS+P P+G  KG QAL+ R+ GNKAA YNCKF G+QDT+CDD G H
Sbjct: 150 VLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNH 209

Query: 191 FYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCSIT 250
           F+KDC+++GT DFIFG G SL+L TQ+NVVG  G+ V+ AH+ +   + SGY F HC +T
Sbjct: 210 FFKDCYIEGTFDFIFGSGRSLYLGTQLNVVG-DGIRVITAHAGKSAAEKSGYSFVHCKVT 269

Query: 251 GTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLGAV 310
           GT G   YLGRSW    +VV+AYT ++ +++P GW +      D TVFYGEYKC+G G+ 
Sbjct: 270 GT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSH 329

Query: 311 STGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPP 345
              R K+ + +   + K FI+L ++    WLLPPP
Sbjct: 330 KEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPP 336

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 2.8e-95
Identity = 165/338 (48.82%), Postives = 223/338 (65.98%), Query Frame = 0


Query: 8   TTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAI 67
           T  +P  + ++  WF  +VKP + RK  LDPAL AAE +  ++ V   G  +FKT+ +AI
Sbjct: 25  TDPIPENRAQIPQWFKTNVKPYSQRKGTLDPALEAAEAARQIITVNQKGGANFKTLNEAI 84

Query: 68  ASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSP 127
            S+P GN  RV+I +  GVY EKV ID  +PFITL G P     LT+ G A +YGT+ S 
Sbjct: 85  KSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAETVLTYHGTAAQYGTVESA 144

Query: 128 TLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 187
           TLIV A+YF AA+L I+N++P P+    GQALA R+  +KAA Y+C+F GFQDTLCDD G
Sbjct: 145 TLIVWAEYFQAAHLTIKNTAPMPKPGSQGQALAMRINADKAAFYSCRFHGFQDTLCDDKG 204

Query: 188 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 247
            HF+KDC+++GT DFIFG+G SL+LNTQ++ VG  GL V+ A  R+   + +GY F HC 
Sbjct: 205 NHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVG-DGLRVITAQGRQSATEQNGYTFVHCK 264

Query: 248 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 307
           +TGT G   YLGRSW    +VV+A+T +  +++P GW +  N  YD TVFYGEYKC G G
Sbjct: 265 VTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPG 324

Query: 308 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPK 346
           +    R  + + + K +V PF+ L ++    WLLPPPK
Sbjct: 325 SHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 3.1e-94
Identity = 162/336 (48.21%), Postives = 223/336 (66.37%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +PA++ ++  WF  +VKP + R+  LDP L AAE S  V+ V  +G GDFKTI  AI 
Sbjct: 26  TPIPADRAQIPQWFMANVKPFSQRRGTLDPELEAAEASRRVIIVNQNGGGDFKTINAAIK 85

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           S+P+ N  RV+I +  G+Y EKV +D  +P++TL G P    NLT+ G A KYGT+ S T
Sbjct: 86  SIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPGAETNLTYAGTAAKYGTVESAT 145

Query: 129 LIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDGL 188
           LIV A  F+AANL I N+SP P+    GQALA R+ G+KAA YNC+F GFQDTLCDD G 
Sbjct: 146 LIVWATNFLAANLNIINTSPMPKPGTQGQALAMRINGDKAAFYNCRFYGFQDTLCDDRGN 205

Query: 189 HFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCSI 248
           HF+K+C+++GT DFIFG+G SL+L TQ++ VG  GL V+AAH+R+   + +GY F HC +
Sbjct: 206 HFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG-DGLRVIAAHNRQSTTEQNGYSFVHCKV 265

Query: 249 TGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLGA 308
           TG  G   YLGR+W    +VV++YT ++ +++P GW +    A+D TVFYGEY C+G G+
Sbjct: 266 TGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGS 325

Query: 309 VSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPP 345
               R    + +   +   F+ L ++   KWLLPPP
Sbjct: 326 HKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

[Close]

BLAST of Cp4.1LG16g08430 vs. NCBI nr
Match: XP_023512285.1 (probable pectinesterase 48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 719 bits (1856), Expect = 1.03e-261
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK
Sbjct: 75  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD
Sbjct: 135 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE
Sbjct: 255 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL
Sbjct: 315 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. NCBI nr
Match: XP_022943549.1 (probable pectinesterase 48 [Cucurbita moschata] >KAG6570447.1 putative pectinesterase 48, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 705 bits (1819), Expect = 4.52e-256
Identity = 341/346 (98.55%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPISCATTEVPAEK RLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISCATTEVPAEKSRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KTITKAIASVPVGNTKRVVIWIGGGVYKEKV I+RNKPFITLYGSPNNMPNLTFDGDATK
Sbjct: 75  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVTINRNKPFITLYGSPNNMPNLTFDGDATK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGTIYS TLIVEADYFVAANLVIENSSPRPEGIKGGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTIYSATLIVEADYFVAANLVIENSSPRPEGIKGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE
Sbjct: 255 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL
Sbjct: 315 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. NCBI nr
Match: XP_022986221.1 (probable pectinesterase 48 [Cucurbita maxima])

HSP 1 Score: 700 bits (1807), Expect = 3.05e-254
Identity = 337/346 (97.40%), Postives = 342/346 (98.84%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPISCAT+EVPAEK RLE+WFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISCATSEVPAEKSRLESWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KTITKAIASVPVGNTKRVVIWIGGGVYKEKV IDRNKPFITLYGSPNNMPNLTFDGDATK
Sbjct: 75  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVTIDRNKPFITLYGSPNNMPNLTFDGDATK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEADYFVAANLVIENSSPRPEGIKGGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEADYFVAANLVIENSSPRPEGIKGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNT MNVVGIGGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTHMNVVGIGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE
Sbjct: 255 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           YKCSGLGAVSTGRAKFGKQL+KAQVKPFINLDFVHAHKWLLPPPKL
Sbjct: 315 YKCSGLGAVSTGRAKFGKQLSKAQVKPFINLDFVHAHKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. NCBI nr
Match: XP_022985663.1 (probable pectinesterase 48 [Cucurbita maxima])

HSP 1 Score: 626 bits (1615), Expect = 5.57e-225
Identity = 298/346 (86.13%), Postives = 325/346 (93.93%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPIS ATTEVPAEK +LEAWFS HVKPLAARKA+LDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KT+T+AIASVP GNTKRVVIWIG GVYKEK+ I+RNKPFITLYGSPNNMPNLTFDGDA K
Sbjct: 75  KTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNKPFITLYGSPNNMPNLTFDGDAKK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEA+YFVAANLVIENSSPRP+G +GGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEAEYFVAANLVIENSSPRPDGREGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLH YKDCF+QGTVDFIFGK T+L+LN+Q+NVVG+GGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVVGVGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGG NTYLGRSWRPWSRVVFAYT+IADILHPEGWDDM+NPA+D TVF+GE
Sbjct: 255 YVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMSNPAFDKTVFFGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           +KCSG GA ++ R KF KQL++AQ KPFINLD+V A KWLLPPPKL
Sbjct: 315 FKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. NCBI nr
Match: XP_022985661.1 (probable pectinesterase 48 [Cucurbita maxima])

HSP 1 Score: 622 bits (1605), Expect = 1.86e-223
Identity = 295/346 (85.26%), Postives = 323/346 (93.35%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPIS ATTEVPAEK +LEAWFS HVKPLAARKA+LDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KT+T+AIASVP GNTKRVVIWIG GVYKEK+ I+RNKPFITLYGSPNNMPNLTFDGDA K
Sbjct: 75  KTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNKPFITLYGSPNNMPNLTFDGDAKK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEA+YFVAANLVIEN+SPRP+  KGG ALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLH YKDCF+QGTVDFIFGK T+L+LN+Q+NV+G+GGLGV+AAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVLGVGGLGVIAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGG NTYLGRSWRPWSRVVFAYT+IADILHPEGWDDMNNPA+D TVF+GE
Sbjct: 255 YVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNPAFDKTVFFGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           +KCSG GA ++ R KF KQL++AQ KPFINLD+V A KWLLPPPKL
Sbjct: 315 FKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL
Match: A0A6J1FUN3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448286 PE=4 SV=1)

HSP 1 Score: 705 bits (1819), Expect = 2.19e-256
Identity = 341/346 (98.55%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPISCATTEVPAEK RLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISCATTEVPAEKSRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KTITKAIASVPVGNTKRVVIWIGGGVYKEKV I+RNKPFITLYGSPNNMPNLTFDGDATK
Sbjct: 75  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVTINRNKPFITLYGSPNNMPNLTFDGDATK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGTIYS TLIVEADYFVAANLVIENSSPRPEGIKGGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTIYSATLIVEADYFVAANLVIENSSPRPEGIKGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE
Sbjct: 255 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL
Sbjct: 315 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL
Match: A0A6J1JAI1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484040 PE=4 SV=1)

HSP 1 Score: 700 bits (1807), Expect = 1.48e-254
Identity = 337/346 (97.40%), Postives = 342/346 (98.84%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPISCAT+EVPAEK RLE+WFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISCATSEVPAEKSRLESWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KTITKAIASVPVGNTKRVVIWIGGGVYKEKV IDRNKPFITLYGSPNNMPNLTFDGDATK
Sbjct: 75  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVTIDRNKPFITLYGSPNNMPNLTFDGDATK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEADYFVAANLVIENSSPRPEGIKGGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEADYFVAANLVIENSSPRPEGIKGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNT MNVVGIGGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTHMNVVGIGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE
Sbjct: 255 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           YKCSGLGAVSTGRAKFGKQL+KAQVKPFINLDFVHAHKWLLPPPKL
Sbjct: 315 YKCSGLGAVSTGRAKFGKQLSKAQVKPFINLDFVHAHKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL
Match: A0A6J1JEA4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483653 PE=4 SV=1)

HSP 1 Score: 626 bits (1615), Expect = 2.70e-225
Identity = 298/346 (86.13%), Postives = 325/346 (93.93%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPIS ATTEVPAEK +LEAWFS HVKPLAARKA+LDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KT+T+AIASVP GNTKRVVIWIG GVYKEK+ I+RNKPFITLYGSPNNMPNLTFDGDA K
Sbjct: 75  KTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNKPFITLYGSPNNMPNLTFDGDAKK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEA+YFVAANLVIENSSPRP+G +GGQALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEAEYFVAANLVIENSSPRPDGREGGQALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLH YKDCF+QGTVDFIFGK T+L+LN+Q+NVVG+GGLGVVAAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVVGVGGLGVVAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGG NTYLGRSWRPWSRVVFAYT+IADILHPEGWDDM+NPA+D TVF+GE
Sbjct: 255 YVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMSNPAFDKTVFFGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           +KCSG GA ++ R KF KQL++AQ KPFINLD+V A KWLLPPPKL
Sbjct: 315 FKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL
Match: A0A6J1J8V7 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483650 PE=4 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 9.00e-224
Identity = 295/346 (85.26%), Postives = 323/346 (93.35%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPIS ATTEVPAEK +LEAWFS HVKPLAARKA+LDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KT+T+AIASVP GNTKRVVIWIG GVYKEK+ I+RNKPFITLYGSPNNMPNLTFDGDA K
Sbjct: 75  KTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNKPFITLYGSPNNMPNLTFDGDAKK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEA+YFVAANLVIEN+SPRP+  KGG ALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLH YKDCF+QGTVDFIFGK T+L+LN+Q+NV+G+GGLGV+AAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVLGVGGLGVIAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGG NTYLGRSWRPWSRVVFAYT+IADILHPEGWDDMNNPA+D TVF+GE
Sbjct: 255 YVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNPAFDKTVFFGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           +KCSG GA ++ R KF KQL++AQ KPFINLD+V A KWLLPPPKL
Sbjct: 315 FKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL
Match: A0A6J1J5H3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483652 PE=4 SV=1)

HSP 1 Score: 621 bits (1601), Expect = 3.66e-223
Identity = 294/346 (84.97%), Postives = 323/346 (93.35%), Query Frame = 0


Query: 1   FAPISCATTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDF 60
           FAPIS ATTEVPAEK +LEAWFS HVKPLAARKA+LDPALVAAEESSTVVKVRADGSGDF
Sbjct: 15  FAPISSATTEVPAEKSQLEAWFSGHVKPLAARKAELDPALVAAEESSTVVKVRADGSGDF 74

Query: 61  KTITKAIASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATK 120
           KT+T+AIASVP GNTKRVVIWIG GVYKEK+ I+RN+PFITLYGSPNNMPNLTFDGDA K
Sbjct: 75  KTVTEAIASVPAGNTKRVVIWIGEGVYKEKLTIERNQPFITLYGSPNNMPNLTFDGDAKK 134

Query: 121 YGTIYSPTLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQD 180
           YGT+YS TLIVEA+YFVAANLVIEN+SPRP+  KGG ALAAR+RGNKAAIYNCKFIGFQD
Sbjct: 135 YGTVYSATLIVEAEYFVAANLVIENTSPRPDARKGGPALAARIRGNKAAIYNCKFIGFQD 194

Query: 181 TLCDDDGLHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSG 240
           TLCDDDGLH YKDCF+QGTVDFIFGK T+L+LN+Q+NV+G+GGLGV+AAHSREKEDDPSG
Sbjct: 195 TLCDDDGLHVYKDCFIQGTVDFIFGKATALYLNSQLNVLGVGGLGVIAAHSREKEDDPSG 254

Query: 241 YVFAHCSITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGE 300
           YVFAHCSITGTGG NTYLGRSWRPWSRVVFAYT+IADILHPEGWDDMNNPA+D TVF+GE
Sbjct: 255 YVFAHCSITGTGGPNTYLGRSWRPWSRVVFAYTTIADILHPEGWDDMNNPAFDKTVFFGE 314

Query: 301 YKCSGLGAVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 346
           +KCSG GA ++ R KF KQL++AQ KPFINLD+V A KWLLPPPKL
Sbjct: 315 FKCSGPGAETSKRVKFSKQLSEAQAKPFINLDYVQAQKWLLPPPKL 360

[Close]

BLAST of Cp4.1LG16g08430 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 386.0 bits (990), Expect = 3.3e-107
Identity = 183/339 (53.98%), Postives = 242/339 (71.39%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +P  K ++  WF+ HV PLA RK  LDPALVAAE +  ++ V   G G+FKT+T AI 
Sbjct: 27  TPIPEGKPQVVQWFNTHVGPLAQRKG-LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 86

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           SVP GNTKRV+I +  G Y+EKV IDRNKPFITL G PN MP +T+DG A KYGT+ S +
Sbjct: 87  SVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSAS 146

Query: 129 LIVEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 188
           LI+ +DYF+A N+V++N++P P+G  KG QAL+ R+ GN AA YNCKF GFQDT+CDD G
Sbjct: 147 LIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTG 206

Query: 189 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 248
            HF+KDC+V+GT DFIFG GTS++L TQ++VVG  G+ V+AAH+ +  ++ SGY F HC 
Sbjct: 207 NHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG-DGIRVIAAHAGKSAEEKSGYSFVHCK 266

Query: 249 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 308
           +TGTGG   YLGR+W    +VV+AYT +  +++P GW +   PA+D TVFYGEYKCSG G
Sbjct: 267 VTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPG 326

Query: 309 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 347
           +    R  F + +   +   F++L ++   KWLLPPP L
Sbjct: 327 SHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Cp4.1LG16g08430 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 385.2 bits (988), Expect = 5.6e-107
Identity = 183/339 (53.98%), Postives = 242/339 (71.39%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +P  K ++  WF+ +V PLA RK  LDPALVAAE +  ++ V   G G+FKT+T AI 
Sbjct: 27  TPIPEGKPQVAQWFNANVGPLAQRKG-LDPALVAAEAAPRIINVNPKG-GEFKTLTDAIK 86

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           SVP GNTKRV+I +  G YKEKV IDRNKPFITL G PN MP +T+DG A KYGT+ S +
Sbjct: 87  SVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAMPVITYDGTAAKYGTVDSAS 146

Query: 129 LIVEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 188
           LI+ +DYF+A N+V++N++P P+G  KG QAL+ R+ GN AA YNCKF GFQDT+CDD G
Sbjct: 147 LIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTG 206

Query: 189 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 248
            HF+KDC+V+GT DFIFG GTS++L TQ++VVG  G+ V+AAH+ +  ++ SGY F HC 
Sbjct: 207 NHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG-DGIRVIAAHAGKSAEEKSGYSFVHCK 266

Query: 249 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 308
           +TGTGG   YLGR+W    +VV+AYT +  +++P GW +   PA+D TVFYGEYKCSG G
Sbjct: 267 VTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPG 326

Query: 309 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPKL 347
           +    R  F + +   +   F++L ++   KWLLPPP L
Sbjct: 327 SHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPPAL 361

[Close]

BLAST of Cp4.1LG16g08430 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 4.7e-98
Identity = 171/335 (51.04%), Postives = 223/335 (66.57%), Query Frame = 0


Query: 11  VPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIASV 70
           +P  K R+E WF+ +VK                           +G G FKTIT+AI SV
Sbjct: 30  IPENKGRIEQWFNTNVK--------------------------QNGRGHFKTITEAINSV 89

Query: 71  PVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPTLI 130
             GNT+RV+I IG GVYKEKV IDR+KPFITLYG PN MP LTFDG A +YGT+ S TLI
Sbjct: 90  RAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAMPVLTFDGTAAQYGTVDSATLI 149

Query: 131 VEADYFVAANLVIENSSPRPEG-IKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDGLH 190
           V +DYF+A N++++NS+P P+G  KG QAL+ R+ GNKAA YNCKF G+QDT+CDD G H
Sbjct: 150 VLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNH 209

Query: 191 FYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCSIT 250
           F+KDC+++GT DFIFG G SL+L TQ+NVVG  G+ V+ AH+ +   + SGY F HC +T
Sbjct: 210 FFKDCYIEGTFDFIFGSGRSLYLGTQLNVVG-DGIRVITAHAGKSAAEKSGYSFVHCKVT 269

Query: 251 GTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLGAV 310
           GT G   YLGRSW    +VV+AYT ++ +++P GW +      D TVFYGEYKC+G G+ 
Sbjct: 270 GT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSH 329

Query: 311 STGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPP 345
              R K+ + +   + K FI+L ++    WLLPPP
Sbjct: 330 KEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPP 336

[Close]

BLAST of Cp4.1LG16g08430 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 2.0e-96
Identity = 165/338 (48.82%), Postives = 223/338 (65.98%), Query Frame = 0


Query: 8   TTEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAI 67
           T  +P  + ++  WF  +VKP + RK  LDPAL AAE +  ++ V   G  +FKT+ +AI
Sbjct: 25  TDPIPENRAQIPQWFKTNVKPYSQRKGTLDPALEAAEAARQIITVNQKGGANFKTLNEAI 84

Query: 68  ASVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSP 127
            S+P GN  RV+I +  GVY EKV ID  +PFITL G P     LT+ G A +YGT+ S 
Sbjct: 85  KSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAETVLTYHGTAAQYGTVESA 144

Query: 128 TLIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDG 187
           TLIV A+YF AA+L I+N++P P+    GQALA R+  +KAA Y+C+F GFQDTLCDD G
Sbjct: 145 TLIVWAEYFQAAHLTIKNTAPMPKPGSQGQALAMRINADKAAFYSCRFHGFQDTLCDDKG 204

Query: 188 LHFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCS 247
            HF+KDC+++GT DFIFG+G SL+LNTQ++ VG  GL V+ A  R+   + +GY F HC 
Sbjct: 205 NHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVG-DGLRVITAQGRQSATEQNGYTFVHCK 264

Query: 248 ITGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLG 307
           +TGT G   YLGRSW    +VV+A+T +  +++P GW +  N  YD TVFYGEYKC G G
Sbjct: 265 VTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNRGYDKTVFYGEYKCFGPG 324

Query: 308 AVSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPPK 346
           +    R  + + + K +V PF+ L ++    WLLPPPK
Sbjct: 325 SHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPPK 360

[Close]

BLAST of Cp4.1LG16g08430 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 2.2e-95
Identity = 162/336 (48.21%), Postives = 223/336 (66.37%), Query Frame = 0


Query: 9   TEVPAEKWRLEAWFSEHVKPLAARKADLDPALVAAEESSTVVKVRADGSGDFKTITKAIA 68
           T +PA++ ++  WF  +VKP + R+  LDP L AAE S  V+ V  +G GDFKTI  AI 
Sbjct: 26  TPIPADRAQIPQWFMANVKPFSQRRGTLDPELEAAEASRRVIIVNQNGGGDFKTINAAIK 85

Query: 69  SVPVGNTKRVVIWIGGGVYKEKVRIDRNKPFITLYGSPNNMPNLTFDGDATKYGTIYSPT 128
           S+P+ N  RV+I +  G+Y EKV +D  +P++TL G P    NLT+ G A KYGT+ S T
Sbjct: 86  SIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPGAETNLTYAGTAAKYGTVESAT 145

Query: 129 LIVEADYFVAANLVIENSSPRPEGIKGGQALAARMRGNKAAIYNCKFIGFQDTLCDDDGL 188
           LIV A  F+AANL I N+SP P+    GQALA R+ G+KAA YNC+F GFQDTLCDD G 
Sbjct: 146 LIVWATNFLAANLNIINTSPMPKPGTQGQALAMRINGDKAAFYNCRFYGFQDTLCDDRGN 205

Query: 189 HFYKDCFVQGTVDFIFGKGTSLHLNTQMNVVGIGGLGVVAAHSREKEDDPSGYVFAHCSI 248
           HF+K+C+++GT DFIFG+G SL+L TQ++ VG  GL V+AAH+R+   + +GY F HC +
Sbjct: 206 HFFKNCYIEGTYDFIFGRGASLYLTTQLHAVG-DGLRVIAAHNRQSTTEQNGYSFVHCKV 265

Query: 249 TGTGGKNTYLGRSWRPWSRVVFAYTSIADILHPEGWDDMNNPAYDHTVFYGEYKCSGLGA 308
           TG  G   YLGR+W    +VV++YT ++ +++P GW +    A+D TVFYGEY C+G G+
Sbjct: 266 TGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQENRVRAHDKTVFYGEYMCTGPGS 325

Query: 309 VSTGRAKFGKQLTKAQVKPFINLDFVHAHKWLLPPP 345
               R    + +   +   F+ L ++   KWLLPPP
Sbjct: 326 HKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g08430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LY19	4.6e-106	53.98	Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2	[more]
Q84WM7	7.9e-106	53.98	Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1	[more]
Q9FKF3	6.7e-97	51.04	Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2	[more]
Q9LY17	2.8e-95	48.82	Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1	[more]
Q9LY18	3.1e-94	48.21	Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1	[more]

BLAST of Cp4.1LG16g08430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023512285.1	1.03e-261	100.00	probable pectinesterase 48 [Cucurbita pepo subsp. pepo]	[more]
XP_022943549.1	4.52e-256	98.55	probable pectinesterase 48 [Cucurbita moschata] >KAG6570447.1 putative pectinest...	[more]
XP_022986221.1	3.05e-254	97.40	probable pectinesterase 48 [Cucurbita maxima]	[more]
XP_022985663.1	5.57e-225	86.13	probable pectinesterase 48 [Cucurbita maxima]	[more]
XP_022985661.1	1.86e-223	85.26	probable pectinesterase 48 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG16g08430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUN3	2.19e-256	98.55	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111448286 PE=4 SV=1	[more]
A0A6J1JAI1	1.48e-254	97.40	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484040 PE=4 SV=1	[more]
A0A6J1JEA4	2.70e-225	86.13	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483653 PE=4 SV=1	[more]
A0A6J1J8V7	9.00e-224	85.26	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483650 PE=4 SV=1	[more]
A0A6J1J5H3	3.66e-223	84.97	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483652 PE=4 SV=1	[more]

BLAST of Cp4.1LG16g08430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07410.1	3.3e-107	53.98	Pectin lyase-like superfamily protein	[more]
AT1G69940.1	5.6e-107	53.98	Pectin lyase-like superfamily protein	[more]
AT5G61680.1	4.7e-98	51.04	Pectin lyase-like superfamily protein	[more]
AT5G07430.1	2.0e-96	48.82	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	2.2e-95	48.21	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 43..342 e-value: 1.4E-93 score: 315.7
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 50..335 e-value: 1.3E-58 score: 198.0
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 15..341
None	No IPR available	PANTHER	PTHR31321:SF87	PECTINESTERASE 63-RELATED	coord: 15..341
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 196..205
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 46..340

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG16g08430.1	Cp4.1LG16g08430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity