Cp4.1LG16g07290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g07290
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type domain-containing protein
LocationCp4.1LG16: 7371285 .. 7373440 (-)
RNA-Seq ExpressionCp4.1LG16g07290
SyntenyCp4.1LG16g07290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATATATGGCCGTTTCATTCTTCTCTCTCTGAACGGAATTTTGTTCTTCTTGTACAGATTGAAAGACTCTGCTGAGGGAACCCAAATTTCAAGTTCCGAGAACAACCAATCAATTTGTTTCGGTACGCAGCGATTGAAGCAAAACAAGGAATAAAGAAGAAAACAGAGAGATCGGTTTCATTTCGCTTTGAATCAGATTGTTATTGACAGAGATCATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGACTCGTTTCCTAAGGCGGTGGGAGCGATGCCGGCGTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGACAGCGCTATATCTTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATACTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAAGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATGTACGGTACCGAACGCTGTTTTTAATTTATTGATATATATAAATAGGAGAAGTAGTGGTTGAGAAACGTTGTGGTGGTTTAATTAACTGACGTATATACTTGTATGGAAGCAGATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGATAAGGATTATGGAGGCGATAGAAGTAGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAAGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACGCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGAACAACAAAAAAACACACACACATATTTCATTCTTCTCTTCAAATTCTTCAAATTCTCCACCCTTTTTCTACACATTTCTAATCTCTGATCAGCCTCTGTTTATCCTCTCTCAAATTTGTGTCATTTTCTTACACAAATTTCTCTAATCTCTTTATGCTTTGATTGAATCCGATGATGATGGGTATAGTGGAGACATGGGGGGAGCGGACGTAGCGTATTTTAGCGAAATTCAGGCGTATGTTAGTATTAAAATTATAAAGGAAAAAAAGGAACGGAGGAGGAGGTTTAAAAGCTGACACGTCGGACTCCCGGAAATATCGGTGTTGGAGAGCGGCAGTGAGGTAGGGCCCACATCCTTTTTACCTTACTCTTTTCACACGGATTCCAATTCCTAATGCTTCCAAAATTAATTAATTATTTATTTATTTATTTATTCGACAATTTTATGGGTTACTCTAGTAATTATGGGAATCTAATTCTTAAAATAAATAAAAAGTAATGGGTATTGGTTGAGGAGTCGCGTTGGGTCAATTTTATAATTTTGTCTTTTTCAGGGGAGCGAAATGTAAATGAAAACGGAGAGTTTTTTAAGTGGGGTCCATGGGATGGGGGGATTTTGTGTAAAAAGAGAGAGTCAGGGGGGCATGAATGTAAAGTGTTGAAGTATAGGAATACATGATGTGGAGAATTAGAGATTTTGTGGTGTCTTTGTTTATTTTTATTTTTATTTTTTTAATTTTAATTTATTTAGGGGGTTAGGGATAGGGATCTTAGGGTGTTCCTGAGTGGGGCCACCACCTCCACCTGTCCCTTTCATAACCAGCACTCTTGCCATCACATTCTCTCTCTCTCTCTCTCTAACTCTTCTGGGGCCCACAATGATTTTGTCCAAATTCTT

mRNA sequence

TTTATATATGGCCGTTTCATTCTTCTCTCTCTGAACGGAATTTTGTTCTTCTTGTACAGATTGAAAGACTCTGCTGAGGGAACCCAAATTTCAAGTTCCGAGAACAACCAATCAATTTGTTTCGGTACGCAGCGATTGAAGCAAAACAAGGAATAAAGAAGAAAACAGAGAGATCGGTTTCATTTCGCTTTGAATCAGATTGTTATTGACAGAGATCATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGACTCGTTTCCTAAGGCGGTGGGAGCGATGCCGGCGTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGACAGCGCTATATCTTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATACTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAAGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGATAAGGATTATGGAGGCGATAGAAGTAGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAAGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACGCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGAACAACAAAAAAACACACACACATATTTCATTCTTCTCTTCAAATTCTTCAAATTCTCCACCCTTTTTCTACACATTTCTAATCTCTGATCAGCCTCTGTTTATCCTCTCTCAAATTTGTGTCATTTTCTTACACAAATTTCTCTAATCTCTTTATGCTTTGATTGAATCCGATGATGATGGGTATAGTGGAGACATGGGGGGAGCGGACGTAGCGTATTTTAGCGAAATTCAGGCGTATGTTAGTATTAAAATTATAAAGGAAAAAAAGGAACGGAGGAGGAGGTTTAAAAGCTGACACGTCGGACTCCCGGAAATATCGGTGTTGGAGAGCGGCAGTGAGGTAGGGCCCACATCCTTTTTACCTTACTCTTTTCACACGGATTCCAATTCCTAATGCTTCCAAAATTAATTAATTATTTATTTATTTATTTATTCGACAATTTTATGGGTTACTCTAGTAATTATGGGAATCTAATTCTTAAAATAAATAAAAAGTAATGGGTATTGGTTGAGGAGTCGCGTTGGGTCAATTTTATAATTTTGTCTTTTTCAGGGGAGCGAAATGTAAATGAAAACGGAGAGTTTTTTAAGTGGGGTCCATGGGATGGGGGGATTTTGTGTAAAAAGAGAGAGTCAGGGGGGCATGAATGTAAAGTGTTGAAGTATAGGAATACATGATGTGGAGAATTAGAGATTTTGTGGTGTCTTTGTTTATTTTTATTTTTATTTTTTTAATTTTAATTTATTTAGGGGGTTAGGGATAGGGATCTTAGGGTGTTCCTGAGTGGGGCCACCACCTCCACCTGTCCCTTTCATAACCAGCACTCTTGCCATCACATTCTCTCTCTCTCTCTCTCTAACTCTTCTGGGGCCCACAATGATTTTGTCCAAATTCTT

Coding sequence (CDS)

ATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATACTGCAGCGGCGGAGACGACTCTGCTTCCGCCGTACAATTCTGTAGCGATTGACTCGTTTCCTAAGGCGGTGGGAGCGATGCCGGCGTCGGCATCGGCGTCGACGGGTGTTGTTATGAAGTCCGACAGCGCTATATCTTATAATCTTCCGATTCCGAGAAAGCGCGGTAGGGATCATTCCACCATAAACCGATTCCTCTCATATCCTTCCTCGCATACTCATTCTCAGAAGAATTGTGGACGTAATTTATATTTTCTTGGCGAAGATATCTCCATCCTAGTTCAACAGCAGCAATTAGATCTGGAACGATTGATATCGCAACATATGGAGAAGGTGAGGATGGAAATGGAAGAAAAACGGAAGAGAGAAGCGATAAGGATTATGGAGGCGATAGAAGTAGGAATGATGGGGATTTTAAGGAGCAAAGAGGAAGAGATCGAGAAGATGGGGAAACTAAATTGGGAACTAGAAGAACGAGTGAATAGTCTATGCATGGAGAATCAAATATGGCGTGATGTGGCTGAGACGAACGAAGCCACCGCAAATGCTCTCCGGAGCGACCTGGAACTTGTTCTGTTAGACGTGAAAAAGGAGGAGGAGGTGGAGGAGGAGGAGAAGGCAGAGTCGTGCTGCGGAAGCAACGGCGGAGATATGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAAGTTGAAGAAGAGAGGAGAAAAGGGAGGAAGACGAGAAGGAAGGAGGAGGACAGACGGTGCAAGAACTGTTGGAAGGAAGAATCATGCGTGCTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGTTCATCTCTACACCATTGTCCCATCTGCAACGCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAG

Protein sequence

MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNNASVRVIMP
Homology
BLAST of Cp4.1LG16g07290 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 4.9e-51
Identity = 135/298 (45.30%), Postives = 187/298 (62.75%), Query Frame = 0

Query: 14  TAAAETTLLPP-YNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPI-PRKRGRD 73
           T   +T+L  P YN   +D         P   S  + +   SD+ + +  P+  RKR R+
Sbjct: 44  TVECQTSLFNPIYNISPVDRLVHQ-SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE 103

Query: 74  HSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEE 133
            S +      PS++   QKN    L FLG+D+S  VQQ   D++RLIS H+E++RME+EE
Sbjct: 104 ESVVLN----PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEE 163

Query: 134 KRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNE 193
           KRK +  RI+EA+E G+M  LR+K++EI  +GKLN  LEE+V SLC+ENQIWRDVA++NE
Sbjct: 164 KRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNE 223

Query: 194 ATANALRSDLELVLLDVKK---EEEVEEEEKAESCCGSNG-GDMEEEEEEEEEEVEEERR 253
           AT NALRS+L+ VL  V++   EE     + A+SCCGSN  GD EEE  +   E ++ ++
Sbjct: 224 ATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKK 283

Query: 254 KGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNNASVRV 306
             R          C++C K E+ VLLLPCRH+CLC+VCGSSL+ CPIC +   AS+ V
Sbjct: 284 MCRVGM-----SMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Cp4.1LG16g07290 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.8e-48
Identity = 149/328 (45.43%), Postives = 189/328 (57.62%), Query Frame = 0

Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN-RFLSYPSSHTHSQKNCGRN------LYFLGEDISIL--VQQ 121
              P+P RKR RD S  N   L   +  + S  N            FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQH---MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLN 181
           QQ +++R +S H   ME+V+ E+EEKRKR+A  IMEAIE G++  LR KEEE E++GK+N
Sbjct: 162 QQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVN 221

Query: 182 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEK 241
             LEERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ 
Sbjct: 222 HALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDD 281

Query: 242 AESCCGSNGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHL 301
           AESCCGS+ G   EE       +E E +   + RR+   R C+NC +EESCVLLLPCRHL
Sbjct: 282 AESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRR---RMCRNCGEEESCVLLLPCRHL 341

Query: 302 CLCTVCGSSLHHCPICNATNNASVRVIM 308
           CLC VCGSS+H CPIC +  NASV V M
Sbjct: 342 CLCGVCGSSVHTCPICTSPKNASVHVNM 356

BLAST of Cp4.1LG16g07290 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-33
Identity = 91/209 (43.54%), Postives = 129/209 (61.72%), Query Frame = 0

Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ +   +  A++  ++  L++K+EEI +MGKLN
Sbjct: 106 IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 165

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 166 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 225

Query: 227 SCCGS-NGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 226 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 285

Query: 287 LCTVCGSS--LHHCPICNATNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 286 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

BLAST of Cp4.1LG16g07290 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 9.7e-31
Identity = 99/264 (37.50%), Postives = 143/264 (54.17%), Query Frame = 0

Query: 53  KSDSAISYNLPIP-----RKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILV 112
           K++S +SYN  +P     R+R    S  N  +   S    S      N+    E +S + 
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINV----ELVSQIQ 120

Query: 113 QQQQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNW 172
            QQQ +++R ++Q  EK+R+E+E +++ +   +  A++  +   L+ K++EI ++  LNW
Sbjct: 121 NQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLNW 180

Query: 173 ELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL--LDVKKEEEVEEEEKAESCCG 232
            L+ERV SL +ENQIWRD+A+TNEA AN LR++L+ VL  L+         E+ AES CG
Sbjct: 181 VLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSCG 240

Query: 233 SNGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVC 292
           S  GD                  G           CK C + E+ VL+LPCRHLCLCTVC
Sbjct: 241 SCCGD------------------GGGEAVTAVGGGCKRCGEREASVLVLPCRHLCLCTVC 300

Query: 293 GSS--LHHCPICNATNNASVRVIM 308
           G S  L  CP+C+   NASV V M
Sbjct: 301 GGSALLRTCPVCDMVMNASVHVNM 302

BLAST of Cp4.1LG16g07290 vs. NCBI nr
Match: XP_023513323.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580 bits (1496), Expect = 2.40e-208
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240

Query: 241 EVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNN 300
           EVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNN
Sbjct: 241 EVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNN 300

Query: 301 ASVRVIMP 308
           ASVRVIMP
Sbjct: 301 ASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. NCBI nr
Match: XP_023513322.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576 bits (1484), Expect = 1.68e-206
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEE 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEE
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEE 240

Query: 241 EEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATN 300
           EEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATN
Sbjct: 241 EEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATN 300

Query: 301 NASVRVIMP 308
           NASVRVIMP
Sbjct: 301 NASVRVIMP 309

BLAST of Cp4.1LG16g07290 vs. NCBI nr
Match: XP_022943619.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 561 bits (1445), Expect = 1.42e-200
Identity = 302/311 (97.11%), Postives = 303/311 (97.43%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEEE 240

Query: 241 EVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNA 300
           EVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN+
Sbjct: 241 EVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNS 300

Query: 301 TNNASVRVIMP 308
           TNNASVRVIMP
Sbjct: 301 TNNASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. NCBI nr
Match: KAG7010432.1 (putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558 bits (1439), Expect = 1.16e-199
Identity = 301/311 (96.78%), Postives = 302/311 (97.11%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSV IDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVPIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHDHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEEE 240

Query: 241 EVEEERRKGRKTRRKEE---DRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNA 300
           EVEEERRKGRKTRRKEE   DRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN+
Sbjct: 241 EVEEERRKGRKTRRKEEEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNS 300

Query: 301 TNNASVRVIMP 308
           TNNASVRVIMP
Sbjct: 301 TNNASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. NCBI nr
Match: XP_022986010.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita maxima])

HSP 1 Score: 556 bits (1434), Expect = 6.73e-199
Identity = 299/311 (96.14%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEEE 240

Query: 241 EVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNA 300
           EVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN+
Sbjct: 241 EVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNS 300

Query: 301 TNNASVRVIMP 308
           TNNASVRVIMP
Sbjct: 301 TNNASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. ExPASy TrEMBL
Match: A0A6J1FUW1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 561 bits (1445), Expect = 6.87e-201
Identity = 302/311 (97.11%), Postives = 303/311 (97.43%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEEE 240

Query: 241 EVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNA 300
           EVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN+
Sbjct: 241 EVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNS 300

Query: 301 TNNASVRVIMP 308
           TNNASVRVIMP
Sbjct: 301 TNNASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 3.26e-199
Identity = 299/311 (96.14%), Postives = 301/311 (96.78%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEEE 240
           QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEEE
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEEE 240

Query: 241 EVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNA 300
           EVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN+
Sbjct: 241 EVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNS 300

Query: 301 TNNASVRVIMP 308
           TNNASVRVIMP
Sbjct: 301 TNNASVRVIMP 308

BLAST of Cp4.1LG16g07290 vs. ExPASy TrEMBL
Match: A0A6J1FS66 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 4.80e-199
Identity = 302/312 (96.79%), Postives = 303/312 (97.12%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEE 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEE
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEE 240

Query: 241 EEVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN 300
           EEVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN
Sbjct: 241 EEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN 300

Query: 301 ATNNASVRVIMP 308
           +TNNASVRVIMP
Sbjct: 301 STNNASVRVIMP 309

BLAST of Cp4.1LG16g07290 vs. ExPASy TrEMBL
Match: A0A6J1JF86 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 2.28e-197
Identity = 299/312 (95.83%), Postives = 301/312 (96.47%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKA+GAMPASASASTGVVMKSDS ISY
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NLPIPRKRGRDHSTINRFLSYPSSH HSQKNCGRN YFLGEDISILVQQQQLDLERLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 H-MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180
           H MEKVRMEMEEKRKREA RIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME
Sbjct: 121 HQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDMEEEEEEEE 240
           NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM   EEEEE
Sbjct: 181 NQIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSNGGDM---EEEEE 240

Query: 241 EEVEEERRKGRKTRRKE---EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN 300
           EEVEEERRKGRKTRRKE   EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN
Sbjct: 241 EEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICN 300

Query: 301 ATNNASVRVIMP 308
           +TNNASVRVIMP
Sbjct: 301 STNNASVRVIMP 309

BLAST of Cp4.1LG16g07290 vs. ExPASy TrEMBL
Match: A0A6J1G785 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451502 PE=4 SV=1)

HSP 1 Score: 407 bits (1046), Expect = 7.77e-140
Identity = 236/333 (70.87%), Postives = 259/333 (77.78%), Query Frame = 0

Query: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60
           MGYGAPLTGFSAN AAAETTLLPPYN +AIDSFPKA  AM  +A+AST   +KSDS I+Y
Sbjct: 1   MGYGAPLTGFSANAAAAETTLLPPYNYIAIDSFPKAAAAMT-TATASTAAGLKSDSGITY 60

Query: 61  NLPIPRKRGRDHSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120
           NL IPRKR RDHSTINRF SYPS   H QKNCG N  FLGEDIS    QQQ DL+RLISQ
Sbjct: 61  NLRIPRKRARDHSTINRFASYPSP-AHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLISQ 120

Query: 121 HMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180
           HMEKVRMEMEEKRKR+A RI+EAIE+GMM ILRSKEEEIEK+GKLNWELEERVNSLCME 
Sbjct: 121 HMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMET 180

Query: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEE---------------VEEEEKAESCCG 240
           QIWRDVA+TNEATANALRS+LE VL  V+  +                V++   AESCCG
Sbjct: 181 QIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAVDD---AESCCG 240

Query: 241 SNGGDMEEEEE----------EEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLP 300
           SN  D ++ ++          EE EE EE  R+GRKTR+  EDR CKNCWKEESCVLLLP
Sbjct: 241 SNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKTRKGNEDRWCKNCWKEESCVLLLP 300

Query: 301 CRHLCLCTVCGSSLHHCPICNATNNASVRVIMP 308
           CRHLCLCTVCGSSLH CPICN+TNNASVRVIMP
Sbjct: 301 CRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 328

BLAST of Cp4.1LG16g07290 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 203.0 bits (515), Expect = 3.5e-52
Identity = 135/298 (45.30%), Postives = 187/298 (62.75%), Query Frame = 0

Query: 14  TAAAETTLLPP-YNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISYNLPI-PRKRGRD 73
           T   +T+L  P YN   +D         P   S  + +   SD+ + +  P+  RKR R+
Sbjct: 44  TVECQTSLFNPIYNISPVDRLVHQ-SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE 103

Query: 74  HSTINRFLSYPSSHTHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQHMEKVRMEMEE 133
            S +      PS++   QKN    L FLG+D+S  VQQ   D++RLIS H+E++RME+EE
Sbjct: 104 ESVVLN----PSAYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEE 163

Query: 134 KRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMENQIWRDVAETNE 193
           KRK +  RI+EA+E G+M  LR+K++EI  +GKLN  LEE+V SLC+ENQIWRDVA++NE
Sbjct: 164 KRKTQGRRIVEAVEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNE 223

Query: 194 ATANALRSDLELVLLDVKK---EEEVEEEEKAESCCGSNG-GDMEEEEEEEEEEVEEERR 253
           AT NALRS+L+ VL  V++   EE     + A+SCCGSN  GD EEE  +   E ++ ++
Sbjct: 224 ATVNALRSNLQQVLAAVERNRWEEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKK 283

Query: 254 KGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCPICNATNNASVRV 306
             R          C++C K E+ VLLLPCRH+CLC+VCGSSL+ CPIC +   AS+ V
Sbjct: 284 MCRVGM-----SMCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Cp4.1LG16g07290 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 199.9 bits (507), Expect = 3.0e-51
Identity = 149/325 (45.85%), Postives = 189/325 (58.15%), Query Frame = 0

Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN-RFLSYPSSHTHSQKNCGRN------LYFLGEDISIL--VQQ 121
              P+P RKR RD S  N   L   +  + S  N            FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLNWEL 181
           QQ +++R +S HME+V+ E+EEKRKR+A  IMEAIE G++  LR KEEE E++GK+N  L
Sbjct: 162 QQHEIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHAL 221

Query: 182 EERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEKAES 241
           EERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ AES
Sbjct: 222 EERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAES 281

Query: 242 CCGSNGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLCLC 301
           CCGS+ G   EE       +E E +   + RR+   R C+NC +EESCVLLLPCRHLCLC
Sbjct: 282 CCGSSCGGGGEETVRRRVGLEREAQDKAERRRR---RMCRNCGEEESCVLLLPCRHLCLC 341

Query: 302 TVCGSSLHHCPICNATNNASVRVIM 308
            VCGSS+H CPIC +  NASV V M
Sbjct: 342 GVCGSSVHTCPICTSPKNASVHVNM 353

BLAST of Cp4.1LG16g07290 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 194.5 bits (493), Expect = 1.2e-49
Identity = 149/328 (45.43%), Postives = 189/328 (57.62%), Query Frame = 0

Query: 2   GYGAPLTGFSANTAAAETTLLPPYNSV--AIDSFPKAVGAMPASASASTGVVMKSDSAIS 61
           GYG        N  +  TT  PP   V    D+ P   G     A  +T +  +      
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYY---ADGATNLDCEF----- 101

Query: 62  YNLPIP-RKRGRDHSTIN-RFLSYPSSHTHSQKNCGRN------LYFLGEDISIL--VQQ 121
              P+P RKR RD S  N   L   +  + S  N            FLG+DI I   + Q
Sbjct: 102 --FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQ 161

Query: 122 QQLDLERLISQH---MEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLN 181
           QQ +++R +S H   ME+V+ E+EEKRKR+A  IMEAIE G++  LR KEEE E++GK+N
Sbjct: 162 QQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVN 221

Query: 182 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVLLDVKK-------EEEVEEEEK 241
             LEERV SL +ENQIWRD+A+TNEATAN LR++LE VL  VK        E+ + EE+ 
Sbjct: 222 HALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDD 281

Query: 242 AESCCGSNGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHL 301
           AESCCGS+ G   EE       +E E +   + RR+   R C+NC +EESCVLLLPCRHL
Sbjct: 282 AESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRR---RMCRNCGEEESCVLLLPCRHL 341

Query: 302 CLCTVCGSSLHHCPICNATNNASVRVIM 308
           CLC VCGSS+H CPIC +  NASV V M
Sbjct: 342 CLCGVCGSSVHTCPICTSPKNASVHVNM 356

BLAST of Cp4.1LG16g07290 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 91/209 (43.54%), Postives = 129/209 (61.72%), Query Frame = 0

Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ +   +  A++  ++  L++K+EEI +MGKLN
Sbjct: 106 IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 165

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 166 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 225

Query: 227 SCCGS-NGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 226 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 285

Query: 287 LCTVCGSS--LHHCPICNATNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 286 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

BLAST of Cp4.1LG16g07290 vs. TAIR 10
Match: AT5G45100.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.5e-34
Identity = 91/209 (43.54%), Postives = 129/209 (61.72%), Query Frame = 0

Query: 107 VQQQQLDLERLISQHMEKVRMEMEEKRKREAIRIMEAIEVGMMGILRSKEEEIEKMGKLN 166
           +QQQ  +++R ++Q  E +R+E+E +++ +   +  A++  ++  L++K+EEI +MGKLN
Sbjct: 79  IQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKLN 138

Query: 167 WELEERVNSLCMENQIWRDVAETNEATANALRSDLELVL-----LDVKKEEEVEEEEKAE 226
           W L+ERV +L +ENQIWRD+A+TNEATAN LRS+LE VL     LD  +   VEE + AE
Sbjct: 139 WVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDAE 198

Query: 227 SCCGS-NGGDMEEEEEEEEEEVEEERRKGRKTRRKEEDRRCKNCWKEESCVLLLPCRHLC 286
           S CGS +GGD+                          +  CK C +  + VL+LPCRHLC
Sbjct: 199 SSCGSCDGGDV----------------------TAVVNGGCKRCGELTASVLVLPCRHLC 258

Query: 287 LCTVCGSS--LHHCPICNATNNASVRVIM 308
           LCTVCGSS  L  CP+C+    ASV V M
Sbjct: 259 LCTVCGSSALLRTCPVCDMVMTASVHVNM 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LDD14.9e-5145.30Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
F4IDI61.8e-4845.43Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9FHE42.1e-3343.54BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O818519.7e-3137.50E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023513323.12.40e-208100.00probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo su... [more]
XP_023513322.11.68e-20699.68probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo su... [more]
XP_022943619.11.42e-20097.11probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita moschat... [more]
KAG7010432.11.16e-19996.78putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp... [more]
XP_022986010.16.73e-19996.14probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FUW16.87e-20197.11probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch... [more]
A0A6J1JCV53.26e-19996.14probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim... [more]
A0A6J1FS664.80e-19996.79probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 OS=Cucurbita mosch... [more]
A0A6J1JF862.28e-19795.83probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim... [more]
A0A6J1G7857.77e-14070.87probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662... [more]
Match NameE-valueIdentityDescription
AT3G12920.13.5e-5245.30SBP (S-ribonuclease binding protein) family protein [more]
AT1G79110.23.0e-5145.85zinc ion binding [more]
AT1G79110.11.2e-4945.43zinc ion binding [more]
AT5G45100.11.5e-3443.54SBP (S-ribonuclease binding protein) family protein [more]
AT5G45100.21.5e-3443.54SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 152..172
NoneNo IPR availableCOILSCoilCoilcoord: 228..255
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 257..297
e-value: 9.1E-8
score: 31.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 228..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..249
NoneNo IPR availablePANTHERPTHR42647:SF12BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATEDcoord: 15..307
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 230..308
e-value: 4.5E-13
score: 50.4
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 15..307
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 261..296
score: 8.919052

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g07290.1Cp4.1LG16g07290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043067 regulation of programmed cell death
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity