Cp4.1LG16g07210 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g07210
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionglucan endo-1,3-beta-glucosidase 3-like
LocationCp4.1LG16: 7399212 .. 7400242 (+)
RNA-Seq ExpressionCp4.1LG16g07210
SyntenyCp4.1LG16g07210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTCAGAACTCTAATAAGCTCATCTTCTTTTCATGTTTTCTTCAATCAATGAATCCATTAAAGTAGTTCTTGTTCTTCTGTCCTGTTCTTGGATCTTTTCTTTTATGCTTTTCATGTTGTCGACTTCAATTTTGAATGCCTAAAAATGGGTTTGTTGTTTTGTTTGTACCCTTTTCCCCTAAATGGAAACCAGGTTCATCAAGCGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCAACAATCAACATCCCCACTACTTCCAACCCCGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTGCAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAAAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGTACTAATCCCAGTAAGCTATCTTTATCGCTTTCGATAATTAGGGTTTTGGTTAGGTGAGTAATAATTGTGTTTTTTAATGTTGTCACAGGCAGTGGCTCGTGCGAGTACCCATCAACAAGGTGAGCCCTCTGCAACCAGTTTGATCAATAAATTTTTGAAATGAAATTAAAATGTTAGTTGAAATTGGATTTATTTGCATTTGCATTTACAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTTCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAG

mRNA sequence

ATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTTCATCAAGCGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCAACAATCAACATCCCCACTACTTCCAACCCCGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTGCAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAAAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGTACTAATCCCAGCAGTGGCTCGTGCGAGTACCCATCAACAAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTTCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAG

Coding sequence (CDS)

ATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTTCATCAAGCGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCAACAATCAACATCCCCACTACTTCCAACCCCGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTGCAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAAAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGTACTAATCCCAGCAGTGGCTCGTGCGAGTACCCATCAACAAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTTCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAG

Protein sequence

MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINIPTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF
Homology
BLAST of Cp4.1LG16g07210 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-18
Identity = 55/126 (43.65%), Postives = 72/126 (57.14%), Query Frame = 0

Query: 51  PITTVPTINIPTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYAC 110
           P+ T PT ++PT+S+P    PT             G   WC+    A+ A LQ  +DY C
Sbjct: 11  PVPT-PTPSVPTSSSPVPKPPT------------QGNKKWCVPKAEATDAQLQSNIDYVC 70

Query: 111 GMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-KNPLPNSCNFGGTAVITSTNPSSG 170
              G DC  IQ  G+C+NPN+VR HASYA NS+YQ K      C+F GT  ITS++PS+G
Sbjct: 71  SQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNG 123

Query: 171 SCEYPS 176
           SC + S
Sbjct: 131 SCSFLS 123

BLAST of Cp4.1LG16g07210 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 8.8e-18
Identity = 55/120 (45.83%), Postives = 74/120 (61.67%), Query Frame = 0

Query: 88  SSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-K 147
           +SWC+   G S +VLQ  LDYACG  GADC+     GSC+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACG-NGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKK 78

Query: 148 NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASPSGPSTSSS 207
                SCNF GTA +T+T+PS   C +PS+++ SS       SGST      + P  S+S
Sbjct: 79  GQASESCNFTGTATLTTTDPSYTGCAFPSSASGSS------GSGSTTVTPGKNSPKGSNS 131

BLAST of Cp4.1LG16g07210 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 91.7 bits (226), Expect = 1.2e-17
Identity = 54/116 (46.55%), Postives = 71/116 (61.21%), Query Frame = 0

Query: 85  TGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYY 144
           T   ++CIA  G     LQ ALD+ACG G ++CS IQ G SCY PN+V+ HAS+AFNSYY
Sbjct: 376 TTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYY 435

Query: 145 QK-NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASPS 200
           QK      SC+F G A+IT+T+PS GSC +P +    +   T  +S     G + S
Sbjct: 436 QKEGRASGSCDFKGVAMITTTDPSHGSCIFPGSKKVGNRTQTVVNSTEVAAGEATS 491

BLAST of Cp4.1LG16g07210 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 1.5e-17
Identity = 55/128 (42.97%), Postives = 77/128 (60.16%), Query Frame = 0

Query: 85  TGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYY 144
           T   ++CIA     + +LQ ALD+ACG G  DCSA+  G SCY P+ V  H++YAFN+YY
Sbjct: 355 TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYY 414

Query: 145 QK-NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASPSGPST 204
           QK      SC+F G A +T+T+PS G+C +P ++ S+  L      G+     +PS  ST
Sbjct: 415 QKMGKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTL------GNNTSALAPSANST 474

Query: 205 SSSA-PKY 211
           +S   PKY
Sbjct: 475 TSGCIPKY 476

BLAST of Cp4.1LG16g07210 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.6e-17
Identity = 56/122 (45.90%), Postives = 77/122 (63.11%), Query Frame = 0

Query: 88  SSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-K 147
           +SWC+   G S  VLQ  LDYACG  GADC+  +   SC+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDTVLQATLDYACG-NGADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKK 78

Query: 148 NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASPSGPSTSSS 207
              P SCNF GTA  T+++PS   C +P TS S S  +TT + G+T    +P G  T+++
Sbjct: 79  GQSPGSCNFDGTATPTNSDPSYTGCAFP-TSASGSSGSTTVTPGTT----NPKGSPTTTT 134

Query: 208 AP 209
            P
Sbjct: 139 LP 134

BLAST of Cp4.1LG16g07210 vs. NCBI nr
Match: XP_023513115.1 (major pollen allergen Ole e 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421 bits (1082), Expect = 4.07e-148
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF 222
           SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF 222

BLAST of Cp4.1LG16g07210 vs. NCBI nr
Match: XP_022943478.1 (major pollen allergen Ole e 10-like [Cucurbita moschata])

HSP 1 Score: 418 bits (1074), Expect = 6.75e-147
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSS ADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF 222
           SILNTTNSSGSTVFGA+PSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 222

BLAST of Cp4.1LG16g07210 vs. NCBI nr
Match: KAG7010427.1 (Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 411 bits (1056), Expect = 4.65e-144
Identity = 220/228 (96.49%), Postives = 220/228 (96.49%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSS ADPTQKQGKYRA SSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAGSSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSS------ALFLLLIAF 222
           SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSS      ALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSSSSSSSALFLLLIAF 228

BLAST of Cp4.1LG16g07210 vs. NCBI nr
Match: KAG6570572.1 (Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 410 bits (1055), Expect = 5.72e-144
Identity = 219/224 (97.77%), Postives = 219/224 (97.77%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSS ADPTQKQGKYRA SSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAGSSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSS--ALFLLLIAF 222
           SILNTTNSSGSTVFGASPSGPSTSSS PKYSSS  ALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSEPKYSSSSSALFLLLIAF 224

BLAST of Cp4.1LG16g07210 vs. NCBI nr
Match: XP_022986028.1 (major pollen allergen Ole e 10-like [Cucurbita maxima])

HSP 1 Score: 404 bits (1038), Expect = 2.15e-141
Identity = 214/223 (95.96%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHC FPGSS ADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCFFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATT TGGSSWCIA+LGASQA LQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSS-ALFLLLIAF 222
           S+LNTTNSSGSTVFGA PSGPSTSSSAPKYSSS ALFLLLIAF
Sbjct: 181 SVLNTTNSSGSTVFGAVPSGPSTSSSAPKYSSSSALFLLLIAF 223

BLAST of Cp4.1LG16g07210 vs. ExPASy TrEMBL
Match: A0A6J1FWZ7 (major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC111448235 PE=4 SV=1)

HSP 1 Score: 418 bits (1074), Expect = 3.27e-147
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSS ADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF 222
           SILNTTNSSGSTVFGA+PSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 222

BLAST of Cp4.1LG16g07210 vs. ExPASy TrEMBL
Match: A0A6J1JFA6 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 PE=4 SV=1)

HSP 1 Score: 404 bits (1038), Expect = 1.04e-141
Identity = 214/223 (95.96%), Postives = 216/223 (96.86%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHC FPGSS ADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCFFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATT TGGSSWCIA+LGASQA LQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSS-ALFLLLIAF 222
           S+LNTTNSSGSTVFGA PSGPSTSSSAPKYSSS ALFLLLIAF
Sbjct: 181 SVLNTTNSSGSTVFGAVPSGPSTSSSAPKYSSSSALFLLLIAF 223

BLAST of Cp4.1LG16g07210 vs. ExPASy TrEMBL
Match: A0A1S4E1R5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC103496317 PE=4 SV=1)

HSP 1 Score: 322 bits (826), Expect = 3.58e-109
Identity = 186/235 (79.15%), Postives = 197/235 (83.83%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGK-YRAA---SSTHQKDITTPITTVP 60
           MDG F  +TLILFLF  IHC  PGSS ADP QKQGK +RAA   SST QKDITTPITTVP
Sbjct: 1   MDGRFIYSTLILFLF--IHCFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVP 60

Query: 61  TINIPT-------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYA 120
           TINIPT       +SNPD+VSP +TTPSFTPATT TGGSSWCIAS  ASQA LQLALDYA
Sbjct: 61  TINIPTIPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYA 120

Query: 121 CGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG 180
           CGMGGADCS+IQ GG+CYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSSG
Sbjct: 121 CGMGGADCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSSG 180

Query: 181 SCEYPSTSTSSSILNTTNSSGSTVFGASPSGPSTSSS--APKYSSSALFLLLIAF 222
           +CEYPSTSTSSSILNTTNSSGSTVFGA PSGPSTSSS  A  + S+  F LL  F
Sbjct: 181 TCEYPSTSTSSSILNTTNSSGSTVFGAVPSGPSTSSSSSALNFPSTQFFSLLTNF 233

BLAST of Cp4.1LG16g07210 vs. ExPASy TrEMBL
Match: A0A6J1D8X8 (glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111018000 PE=4 SV=1)

HSP 1 Score: 278 bits (712), Expect = 3.05e-91
Identity = 158/211 (74.88%), Postives = 170/211 (80.57%), Query Frame = 0

Query: 2   DGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINIP 61
           +  FTCTTLIL LF   HC+ PGSS++   Q Q          QKDITTPITTVPTI+IP
Sbjct: 49  NASFTCTTLILLLFT--HCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIP 108

Query: 62  T------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGA 121
           T      TS PD+VSP + TPSF PATTA GGSSWCIASLGASQA LQLALDYACG+GGA
Sbjct: 109 TMPIVNPTSTPDTVSPAMATPSFGPATTA-GGSSWCIASLGASQAALQLALDYACGVGGA 168

Query: 122 DCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPS 181
           DC  IQ GG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG+CEYPS
Sbjct: 169 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 228

Query: 182 TSTSSSILNTTNSSGSTVFGASPSGPSTSSS 206
           TSTSS++LNTTNSSGSTVFGA P+G   SSS
Sbjct: 229 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSS 249

BLAST of Cp4.1LG16g07210 vs. ExPASy TrEMBL
Match: A0A0A0KDA3 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=1)

HSP 1 Score: 274 bits (701), Expect = 6.07e-91
Identity = 149/187 (79.68%), Postives = 161/187 (86.10%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGK-YRAAS---STHQKDITTPITTVP 60
           MDG F C+TLILFLF  IH   PGSS ADPTQKQGK +RAAS   ST QKDITTPITTVP
Sbjct: 1   MDGRFLCSTLILFLF--IHWFCPGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVP 60

Query: 61  TINIPT-------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYA 120
           TINIPT       +SNPD+VSP +TTPSFTP+TT +GGSSWCIAS  ASQA LQLALDYA
Sbjct: 61  TINIPTIPIINPASSNPDTVSPAMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYA 120

Query: 121 CGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG 176
           CG+GGADCS+IQ GG+CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS+G
Sbjct: 121 CGIGGADCSSIQGGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSTG 180

BLAST of Cp4.1LG16g07210 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 2.0e-41
Identity = 111/232 (47.84%), Postives = 137/232 (59.05%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSS-ADPTQKQGKYRAASSTHQKDITTPITTVPTIN 60
           MD GF   T +L L  F    F G+ +  +P ++           +KDITTP+ T PT  
Sbjct: 1   MDKGFRLVTSLLLLSFF----FSGTKAITEPIEE-----------EKDITTPLATNPT-T 60

Query: 61  IPTTSNPDSVSPT-------ITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGM 120
            PTT  P+S S         +T PS        G  SWC+A    ++  LQ ALDYACG+
Sbjct: 61  TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGI 120

Query: 121 GGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCE 180
           GGADCS IQ GG+CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS GSC 
Sbjct: 121 GGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPSLGSCH 180

Query: 181 YPSTSTSSSILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSAL--FLLLIAF 223
           +PSTSTS SILN T+  G  +FG  PS P+    A   SS  L  FL  + F
Sbjct: 181 FPSTSTSESILNVTSEDGLGLFGRIPSHPTPKPEASTSSSRTLISFLYFLCF 216

BLAST of Cp4.1LG16g07210 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 5.0e-32
Identity = 84/175 (48.00%), Postives = 105/175 (60.00%), Query Frame = 0

Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSS-ADPTQKQGKYRAASSTHQKDITTPITTVPTIN 60
           MD GF   T +L L  F    F G+ +  +P ++           +KDITTP+ T PT  
Sbjct: 1   MDKGFRLVTSLLLLSFF----FSGTKAITEPIEE-----------EKDITTPLATNPT-T 60

Query: 61  IPTTSNPDSVSPT-------ITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGM 120
            PTT  P+S S         +T PS        G  SWC+A    ++  LQ ALDYACG+
Sbjct: 61  TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGI 120

Query: 121 GGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 168
           GGADCS IQ GG+CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS
Sbjct: 121 GGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPS 159

BLAST of Cp4.1LG16g07210 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 121.7 bits (304), Expect = 7.4e-28
Identity = 67/134 (50.00%), Postives = 81/134 (60.45%), Query Frame = 0

Query: 79  TPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASY 138
           T A    G   WCIA   AS   LQ+ALDYACG GGADC  IQ G +CY PN++RDHAS+
Sbjct: 137 TGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASF 196

Query: 139 AFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGASP 198
           AFNSYYQK+P  +SCNFGG A +TST+PS GSC + S+S                 G   
Sbjct: 197 AFNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHFSSSS-----------------GTVS 253

Query: 199 SGPSTSSSAPKYSS 213
           + P +  S P +SS
Sbjct: 257 TSPPSQMSPPDFSS 253

BLAST of Cp4.1LG16g07210 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 114.0 bits (284), Expect = 1.5e-25
Identity = 75/159 (47.17%), Postives = 98/159 (61.64%), Query Frame = 0

Query: 48  ITTPITTVPTINIPT-TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLAL 107
           +T P+T   T   PT  S    V   +  P     + +  G SWC+A  GASQ  LQ AL
Sbjct: 94  VTNPVTNPVTQYPPTQPSGTVPVPVPVVAPPVVSNSPSVSGQSWCVAKPGASQVSLQQAL 153

Query: 108 DYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNP 167
           DYACG+  ADCS +Q GG+CY+P S++ HAS+AFNSYYQKNP P SC+FGG A + +TNP
Sbjct: 154 DYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSPQSCDFGGAASLVNTNP 213

Query: 168 SSGSCEYPSTSTSSSIL--NTTNSSGSTVFGASPSGPST 204
           S+GSC Y + S++S+ +   TT  + ST     P   ST
Sbjct: 214 STGSCIYQTGSSTSTPMTAGTTTPTPSTQTVNQPPVTST 250

BLAST of Cp4.1LG16g07210 vs. TAIR 10
Match: AT5G55180.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 102.1 bits (253), Expect = 6.1e-22
Identity = 51/97 (52.58%), Postives = 63/97 (64.95%), Query Frame = 0

Query: 80  PATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYA 139
           P   +  G +WC+A+   ++  LQ  LDYACG GGADC  IQ G +CYNP S+  HASYA
Sbjct: 362 PVKPSLVGQTWCVANGKTTKEKLQEGLDYACGEGGADCRPIQPGATCYNPESLEAHASYA 421

Query: 140 FNSYYQKNPL-PNSCNFGGTAVITSTNPSSGSCEYPS 176
           FNSYYQKN     +CNFGG A + S  P  G CE+P+
Sbjct: 422 FNSYYQKNARGVGTCNFGGAAYVVSQPPKYGKCEFPT 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84V393.0e-1843.65Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1[more]
Q9SD848.8e-1845.83PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2... [more]
O653991.2e-1746.55Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9ZU911.5e-1742.97Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q9FNQ22.6e-1745.90PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1... [more]
Match NameE-valueIdentityDescription
XP_023513115.14.07e-148100.00major pollen allergen Ole e 10 [Cucurbita pepo subsp. pepo][more]
XP_022943478.16.75e-14799.10major pollen allergen Ole e 10-like [Cucurbita moschata][more]
KAG7010427.14.65e-14496.49Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6570572.15.72e-14497.77Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022986028.12.15e-14195.96major pollen allergen Ole e 10-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FWZ73.27e-14799.10major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC11144823... [more]
A0A6J1JFA61.04e-14195.96major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 ... [more]
A0A1S4E1R53.58e-10979.15PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1D8X83.05e-9174.88glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KDA36.07e-9179.68X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=... [more]
Match NameE-valueIdentityDescription
AT2G30933.12.0e-4147.84Carbohydrate-binding X8 domain superfamily protein [more]
AT2G30933.25.0e-3248.00Carbohydrate-binding X8 domain superfamily protein [more]
AT1G29380.17.4e-2850.00Carbohydrate-binding X8 domain superfamily protein [more]
AT1G09460.11.5e-2547.17Carbohydrate-binding X8 domain superfamily protein [more]
AT5G55180.16.1e-2252.58O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 89..173
e-value: 7.3E-45
score: 165.0
IPR012946X8 domainPFAMPF07983X8coord: 90..159
e-value: 4.6E-20
score: 72.1
NoneNo IPR availableGENE3D1.20.58.1040coord: 84..191
e-value: 2.5E-11
score: 45.8
NoneNo IPR availablePANTHERPTHR31044:SF86CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEINcoord: 16..210
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 16..210

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g07210.1Cp4.1LG16g07210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016020 membrane
cellular_component GO:0009506 plasmodesma
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds