Cp4.1LG16g06240 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g06240
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG16: 6771140 .. 6772288 (-)
RNA-Seq ExpressionCp4.1LG16g06240
SyntenyCp4.1LG16g06240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCGGAACGGCGTCGTATTGGTGTTACCGCTGCAATCGGTTCGTTCGTGCTTGGGTTGAGGATACCATCACTTGCCCTCACTGTGACGGAGGGTTTGTTGAAACGATTGAAACCGCCTCTTCTCTCCCGGCGGCGGATCTCCACCGTCGGCTCTCACCGTCTGCGATTCACACCTTGGATCAAGATCAGTTTCCGACTTCTCGCTTGAGCTCTCGCCGTAGTCGTCGGAGACTTGGGGAACGGACGACCTTTAACCCTATTGTTGTTGTTCGTGGTTCTGCGGATGGCGGCGATGTGGTCGGCGGCGGGGATAGAAATAGCTTCGATATTTACTACGACGATGGTGCTGGGTCTGGTCTTCGACCGGTACCGGCGACCATGTCGGAATTTCTTATGGGGACTGGATTCGATCGCTTGCTGGAGCAGTTGGCTCAACTGGAGATCAACGGGTTAGGTAGATCTGAAAATCCGCCGGCGTCGAAAGCCGCTGTGGAATCAATGCCGACAATCGAGATTTTAGAAAGTCACGTTGGTTCCGATTCGCACTGCGCCGTGTGTAAAGAGGCGTTTGAAATCGGAACGGAAGCCCGGGAAATGCCCTGTAAACACATCTATCACTCCGATTGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGCCCAGTCTGCCGGCACGAGCTGCCATCAGAGCGTGAATCTCCGGCCGGCGGGGTTTCAGATCGGGTTGTTGACGAAGAAACCGTAGGGTTAACGATCTGGAGGCTCCCAGGCGGTGGGTTCGCCGTCGGGCGATTCTCTGGCGGAAGATTAGCCGGAGAAAGAGACCCACCGGCCGTGTACACCGAAATGGACGGTGGATTCAATGCAAACGGAGCTCCAAGAAGGGTGTCGTGGGGTTCAAGAAGAAGCAGGGGAAGAGAGAGCCGTGGAATTGGACGAACATTTCGTAATATCTTTTCCTTTTTTGGGAGATTAAGATCATCAAATTCATCTTCTCCGGCGGTTTCAAATGAATCGGAGTCTGTCAGTAGAAGCCATAGCCATAGCCATAGCCATTCGGGTTCTTCAGTGTTCAACAGATACTTGCGCCGGCAAAACAGAGCATGGGCGATGGAGAACCAGAGCGATAACGATCGATGGTGA

mRNA sequence

ATGGCTACCGGAACGGCGTCGTATTGGTGTTACCGCTGCAATCGGTTCGTTCGTGCTTGGGTTGAGGATACCATCACTTGCCCTCACTGTGACGGAGGGTTTGTTGAAACGATTGAAACCGCCTCTTCTCTCCCGGCGGCGGATCTCCACCGTCGGCTCTCACCGTCTGCGATTCACACCTTGGATCAAGATCAGTTTCCGACTTCTCGCTTGAGCTCTCGCCGTAGTCGTCGGAGACTTGGGGAACGGACGACCTTTAACCCTATTGTTGTTGTTCGTGGTTCTGCGGATGGCGGCGATGTGGTCGGCGGCGGGGATAGAAATAGCTTCGATATTTACTACGACGATGGTGCTGGGTCTGGTCTTCGACCGGTACCGGCGACCATGTCGGAATTTCTTATGGGGACTGGATTCGATCGCTTGCTGGAGCAGTTGGCTCAACTGGAGATCAACGGGTTAGGTAGATCTGAAAATCCGCCGGCGTCGAAAGCCGCTGTGGAATCAATGCCGACAATCGAGATTTTAGAAAGTCACGTTGGTTCCGATTCGCACTGCGCCGTGTGTAAAGAGGCGTTTGAAATCGGAACGGAAGCCCGGGAAATGCCCTGTAAACACATCTATCACTCCGATTGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGCCCAGTCTGCCGGCACGAGCTGCCATCAGAGCGTGAATCTCCGGCCGGCGGGGTTTCAGATCGGGTTGTTGACGAAGAAACCGTAGGGTTAACGATCTGGAGGCTCCCAGGCGGTGGGTTCGCCGTCGGGCGATTCTCTGGCGGAAGATTAGCCGGAGAAAGAGACCCACCGGCCGTGTACACCGAAATGGACGGTGGATTCAATGCAAACGGAGCTCCAAGAAGGGTGTCGTGGGGTTCAAGAAGAAGCAGGGGAAGAGAGAGCCGTGGAATTGGACGAACATTTCGTAATATCTTTTCCTTTTTTGGGAGATTAAGATCATCAAATTCATCTTCTCCGGCGGTTTCAAATGAATCGGAGTCTGTCAGTAGAAGCCATAGCCATAGCCATAGCCATTCGGGTTCTTCAGTGTTCAACAGATACTTGCGCCGGCAAAACAGAGCATGGGCGATGGAGAACCAGAGCGATAACGATCGATGGTGA

Coding sequence (CDS)

ATGGCTACCGGAACGGCGTCGTATTGGTGTTACCGCTGCAATCGGTTCGTTCGTGCTTGGGTTGAGGATACCATCACTTGCCCTCACTGTGACGGAGGGTTTGTTGAAACGATTGAAACCGCCTCTTCTCTCCCGGCGGCGGATCTCCACCGTCGGCTCTCACCGTCTGCGATTCACACCTTGGATCAAGATCAGTTTCCGACTTCTCGCTTGAGCTCTCGCCGTAGTCGTCGGAGACTTGGGGAACGGACGACCTTTAACCCTATTGTTGTTGTTCGTGGTTCTGCGGATGGCGGCGATGTGGTCGGCGGCGGGGATAGAAATAGCTTCGATATTTACTACGACGATGGTGCTGGGTCTGGTCTTCGACCGGTACCGGCGACCATGTCGGAATTTCTTATGGGGACTGGATTCGATCGCTTGCTGGAGCAGTTGGCTCAACTGGAGATCAACGGGTTAGGTAGATCTGAAAATCCGCCGGCGTCGAAAGCCGCTGTGGAATCAATGCCGACAATCGAGATTTTAGAAAGTCACGTTGGTTCCGATTCGCACTGCGCCGTGTGTAAAGAGGCGTTTGAAATCGGAACGGAAGCCCGGGAAATGCCCTGTAAACACATCTATCACTCCGATTGCATAATCCCTTGGCTTTCCATGAGAAATTCCTGCCCAGTCTGCCGGCACGAGCTGCCATCAGAGCGTGAATCTCCGGCCGGCGGGGTTTCAGATCGGGTTGTTGACGAAGAAACCGTAGGGTTAACGATCTGGAGGCTCCCAGGCGGTGGGTTCGCCGTCGGGCGATTCTCTGGCGGAAGATTAGCCGGAGAAAGAGACCCACCGGCCGTGTACACCGAAATGGACGGTGGATTCAATGCAAACGGAGCTCCAAGAAGGGTGTCGTGGGGTTCAAGAAGAAGCAGGGGAAGAGAGAGCCGTGGAATTGGACGAACATTTCGTAATATCTTTTCCTTTTTTGGGAGATTAAGATCATCAAATTCATCTTCTCCGGCGGTTTCAAATGAATCGGAGTCTGTCAGTAGAAGCCATAGCCATAGCCATAGCCATTCGGGTTCTTCAGTGTTCAACAGATACTTGCGCCGGCAAAACAGAGCATGGGCGATGGAGAACCAGAGCGATAACGATCGATGGTGA

Protein sequence

MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHTLDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVGSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSWGSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVFNRYLRRQNRAWAMENQSDNDRW
Homology
BLAST of Cp4.1LG16g06240 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.3e-87
Identity = 188/395 (47.59%), Postives = 247/395 (62.53%), Query Frame = 0

Query: 5   TASYWCYRCNRFVRAWVED------TITCPHCDGGFVETIETASSLPAADLHRRLSPSAI 64
           TASYWCY C RFV  W +       ++ CPHCDGGF+E I  +SS  A +L         
Sbjct: 15  TASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSS-AATEL--------- 74

Query: 65  HTLDQDQFPTSRLSSRRS---RRRLGERTTFNPIVVVRGSA----DGGDVVGGGDRNSFD 124
            T+       S  ++RRS   RRR G R +FNP++V++G A    +G +     DR +F+
Sbjct: 75  -TIPASTEVRSINNNRRSVIRRRRSGRRPSFNPVIVLQGGAGEREEGEEGDAARDRRAFE 134

Query: 125 IYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN--GLGRSENPPASKAAVESM 184
            YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E +  G+GRS NPPASK+A+ES+
Sbjct: 135 FYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESL 194

Query: 185 PTIEILESHVGSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHEL 244
           P +EI + H+GS+++CAVC E FE  TEAREMPCKH++H DCI+PWLS+RNSCPVCR EL
Sbjct: 195 PRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFEL 254

Query: 245 PSERESPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGG- 304
           PSE        S+   ++  VG+TIWRLPGGGFAVGRF+     GER  P V TEMDGG 
Sbjct: 255 PSEPNRR----SNNNEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGG 314

Query: 305 -FNANGAPRRVSW-GSRRSRGRESRGIG----------RTFRNIFSFFGRL---RSSNSS 364
             N+   PRR+SW  S  +   +S G G          R  R + S   R+   RSS+SS
Sbjct: 315 IGNSQDGPRRISWVRSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRPNRSSSSS 374

Query: 365 SPAVSNESESVSRSHSHSHS--HSGSSVFNRYLRR 367
           S A+S  S+++  +           +SV  RY RR
Sbjct: 375 SAAISTSSDNLDLNIEVESRVLDRSNSVMRRYFRR 394

BLAST of Cp4.1LG16g06240 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.3e-84
Identity = 176/378 (46.56%), Postives = 238/378 (62.96%), Query Frame = 0

Query: 3   TGTASYWCYRCNRFVRAWVEDT----ITCPHCDGGFVETIETASSLPAADLHRRLSPSAI 62
           T T SYWCY C RF+  W +      + CP+C+GGF+E IE +S+   A +    S   +
Sbjct: 26  TLTTSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAI--PASTPEV 85

Query: 63  HTLDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGG------DVVGGG---DRNS 122
            ++++    T R   R  RRR   RT+FNP++V+ G   GG      +  G G   +R +
Sbjct: 86  RSVEE----THRSIIR--RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRA 145

Query: 123 FDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEI--NGLGRSENPPASKAAVE 182
           ++ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E   NG+GRS NPPASK+A+E
Sbjct: 146 YEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIE 205

Query: 183 SMPTIEILESHVGSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRH 242
           S+P +EI + H  ++++CAVC E FE G E REMPCKHI+H DCI+PWLS+RNSCPVCR 
Sbjct: 206 SLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRF 265

Query: 243 ELPSERESPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMD- 302
           ELPS+       +     +E  VG+TIWRLPGGGFAVGRF+ G   GER  P V TEMD 
Sbjct: 266 ELPSD------PIQRSNEEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDG 325

Query: 303 GGFNANGAPRRVSW-------GSRRSRGRESRG--IGRTFRNIFSFFGRLRSS--NSSSP 354
           GG  +N  PRR+SW          R+ GR   G  + R  R + SF  R+R S  +S+S 
Sbjct: 326 GGLGSNEGPRRISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSRGSSNSN 385

BLAST of Cp4.1LG16g06240 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 7.4e-81
Identity = 189/410 (46.10%), Postives = 246/410 (60.00%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAAD--------LHR- 60
           MA+G  SYWCY C+RFV  WV D+I+CP CDGGF+E I+       +D         HR 
Sbjct: 1   MASG--SYWCYSCSRFV--WVSDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRS 60

Query: 61  ----------RLSPSAIHTLDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGS--ADGG 120
                       +PSA  ++  D  PT  + +R    R     + NP++V+RGS  A   
Sbjct: 61  PTRFPPPSSSSSTPSA--SMHADNSPTPTIVTRTRSNR-----SPNPVIVLRGSAAAPSS 120

Query: 121 DVVGGG-DRNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN---GLGR 180
           DVV  G DR++F +YYDDG  SGLRP+P +M+EFL+G+GFDRLL+Q++Q+E+N    L  
Sbjct: 121 DVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRS 180

Query: 181 SENPPASKAAVESMPTIEILESHVGSD--SHCAVCKEAFEIGTEAREMPCKHIYHSDCII 240
            E+PPASK+A+E++P IEI  +H+ SD  SHCAVCKE F + + AREMPC HIYH DCI+
Sbjct: 181 CEHPPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCIL 240

Query: 241 PWLSMRNSCPVCRHELPSERESPAGGVSDRVV-------DEETVGLTIWRLPGGGFAVGR 300
           PWL++RNSCPVCRHELP+E  +   G +   V       ++   GLTIWRLPGGGFAVGR
Sbjct: 241 PWLAIRNSCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGR 300

Query: 301 FSGGRLAGERDPPAVYTEMDGG-FNANGAPRRVSWGSRR-------SRGRESRGIGRTFR 360
             GG   G+R  P VYTE+DGG       PRRV+WGSRR       SR R     GR  R
Sbjct: 301 IPGGWRGGDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMR 360

Query: 361 NIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVFNRYLRRQN 369
            +F  F     S +++ A S+ S S  R    + S S  S  +   RR+N
Sbjct: 361 -LFGCFSGSSGSIAAAAAASSGSGSRIRVTRRTRSFSMFSTASSSSRRRN 398

BLAST of Cp4.1LG16g06240 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.0e-37
Identity = 90/240 (37.50%), Postives = 132/240 (55.00%), Query Frame = 0

Query: 7   SYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHTLDQDQF 66
           ++WC+RC R V     D + C +C GGFVE I+   S    D+ R  +   +       F
Sbjct: 7   THWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAF--SAF 66

Query: 67  PTSRLSSRRSRRRLGER---------TTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDG 126
             SRL+ R   R +  R         +   P+++  G A     + GGD +S + +  +G
Sbjct: 67  MRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVEAFV-NG 126

Query: 127 AGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILE 186
           A  G+     T   ++  G G + L+EQL+    +G      PPA K++++++PTI+I +
Sbjct: 127 AAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALPTIKITQ 186

Query: 187 SHV-GSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERES 236
            H+  SDSHC VCK+ FE+ +EA++MPC HIYHSDCI+PWL   NSCPVCR ELPS   S
Sbjct: 187 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSS 236

BLAST of Cp4.1LG16g06240 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.1e-35
Identity = 93/248 (37.50%), Postives = 138/248 (55.65%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M++   ++WC+RC R VR   ++ + C +C GGFVE ++ A + P  D+ R    S    
Sbjct: 1   MSSSRNTHWCHRCQRAVRLHGQEPV-CFYCGGGFVEELDMAQASP-FDMFR----SHRGV 60

Query: 61  LDQDQ----------FPTSRLSSRRSRRRLGERTTFNPIVVVRGSAD----GGDVVG--G 120
           +++DQ          F  +RL+ R   R +  RT  +      G A     GG V     
Sbjct: 61  VERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLT 120

Query: 121 GDRNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKA 180
           GD N+ +  + +G   G+        ++  G G + L EQL+     G  R   PPA ++
Sbjct: 121 GD-NAVEALF-NGGSPGIGITRGNTGDYFFGPGLEELFEQLSA----GTTRRGPPPAPRS 180

Query: 181 AVESMPTIEILESHV-GSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCP 232
           A++++PTI+I + H+  SDS+C VCK+ FE+G+EA++MPC HIYHSDCI+PWL   NSCP
Sbjct: 181 AIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCP 236

BLAST of Cp4.1LG16g06240 vs. NCBI nr
Match: XP_023512263.1 (E3 ubiquitin-protein ligase RDUF2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 767 bits (1980), Expect = 4.44e-279
Identity = 382/382 (100.00%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 34  MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 93

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 94  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 153

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG
Sbjct: 154 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 213

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 214 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 273

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 274 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 333

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF
Sbjct: 334 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 393

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           NRYLRRQNRAWAMENQSDNDRW
Sbjct: 394 NRYLRRQNRAWAMENQSDNDRW 415

BLAST of Cp4.1LG16g06240 vs. NCBI nr
Match: XP_022986887.1 (E3 ubiquitin-protein ligase RDUF2-like [Cucurbita maxima])

HSP 1 Score: 764 bits (1974), Expect = 1.05e-278
Identity = 380/382 (99.48%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF
Sbjct: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           NRYLRRQNRAWA+ENQSDNDRW
Sbjct: 361 NRYLRRQNRAWALENQSDNDRW 382

BLAST of Cp4.1LG16g06240 vs. NCBI nr
Match: KAG6570690.1 (E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 754 bits (1948), Expect = 1.04e-274
Identity = 378/384 (98.44%), Postives = 380/384 (98.96%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEI TEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 181 SDSHCAVCKEAFEIRTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           +DRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 241 ADRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHS--GSS 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESE VSRSHSHSHSHS  GSS
Sbjct: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESEPVSRSHSHSHSHSHSGSS 360

Query: 361 VFNRYLRRQNRAWAMENQSDNDRW 382
           VFNRYLRRQNRAWAMENQSDNDRW
Sbjct: 361 VFNRYLRRQNRAWAMENQSDNDRW 384

BLAST of Cp4.1LG16g06240 vs. NCBI nr
Match: XP_022944700.1 (E3 ubiquitin-protein ligase RDUF2-like [Cucurbita moschata])

HSP 1 Score: 749 bits (1935), Expect = 9.96e-273
Identity = 376/384 (97.92%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSEN PASKAAVESMPTIEILESHVG
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENLPASKAAVESMPTIEILESHVG 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           +DRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 241 ADRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHS--GSS 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSS AVSN+SE VSRSHSHSHSHS  GSS
Sbjct: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSTAVSNQSEPVSRSHSHSHSHSHSGSS 360

Query: 361 VFNRYLRRQNRAWAMENQSDNDRW 382
           VFNRYLRRQNRAWAMENQSDNDRW
Sbjct: 361 VFNRYLRRQNRAWAMENQSDNDRW 384

BLAST of Cp4.1LG16g06240 vs. NCBI nr
Match: KAE8646961.1 (hypothetical protein Csa_020907 [Cucumis sativus])

HSP 1 Score: 685 bits (1767), Expect = 3.19e-247
Identity = 339/382 (88.74%), Postives = 359/382 (93.98%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAW +D+ITCP+CDGGFVE IETASSLP ++LHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPPSNLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQD F + RLS+RRSRRRLG+R+TFNP+VV+RGSAD GDVVGGG+RNSFDIYYDDGAGS
Sbjct: 61  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEING GRSENPPASKAAVESMPTIEILESHV 
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSER SPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFN NG PRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
            SRRSRGRESRGIGRTFRNIFSFFGR RSS+SSS + SNESESVSRSHSHS     SSVF
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHS----SSSVF 360

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           +RYLRRQNRAW +ENQ++N RW
Sbjct: 361 SRYLRRQNRAWVLENQNENGRW 378

BLAST of Cp4.1LG16g06240 vs. ExPASy TrEMBL
Match: A0A6J1J8T8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484489 PE=4 SV=1)

HSP 1 Score: 764 bits (1974), Expect = 5.08e-279
Identity = 380/382 (99.48%), Postives = 382/382 (100.00%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF
Sbjct: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           NRYLRRQNRAWA+ENQSDNDRW
Sbjct: 361 NRYLRRQNRAWALENQSDNDRW 382

BLAST of Cp4.1LG16g06240 vs. ExPASy TrEMBL
Match: A0A6J1FYT8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449083 PE=4 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 4.82e-273
Identity = 376/384 (97.92%), Postives = 379/384 (98.70%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT
Sbjct: 1   MSTGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS
Sbjct: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSEN PASKAAVESMPTIEILESHVG
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENLPASKAAVESMPTIEILESHVG 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           +DRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW
Sbjct: 241 ADRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHS--GSS 360
           GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSS AVSN+SE VSRSHSHSHSHS  GSS
Sbjct: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSTAVSNQSEPVSRSHSHSHSHSHSGSS 360

Query: 361 VFNRYLRRQNRAWAMENQSDNDRW 382
           VFNRYLRRQNRAWAMENQSDNDRW
Sbjct: 361 VFNRYLRRQNRAWAMENQSDNDRW 384

BLAST of Cp4.1LG16g06240 vs. ExPASy TrEMBL
Match: A0A5A7V7N5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00040 PE=4 SV=1)

HSP 1 Score: 683 bits (1763), Expect = 6.28e-247
Identity = 338/382 (88.48%), Postives = 359/382 (93.98%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRC+RFVRAW +D+ITCP+CDGGFVE IETASSLPA +LHRRLSP+AIHT
Sbjct: 1   MSTGTASYWCYRCSRFVRAWAQDSITCPYCDGGFVEAIETASSLPAPNLHRRLSPTAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           +DQD F + RLS+RRSRRR G+R+TFNP+VV+RGSAD GDVVGGG+RNSFDIYYDDGAGS
Sbjct: 61  VDQDSFQSPRLSTRRSRRRFGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEING GRSENPPASKAAVESMPTIEILESHV 
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSER SPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANG PRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGVPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
            SRRSRGRESRGIGRTFRNIFSFFGR RSS+SSS + SNESESVSRSHSHS     SSVF
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHS----SSSVF 360

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           +RYLRRQNRAW +ENQ++N RW
Sbjct: 361 SRYLRRQNRAWVLENQNENGRW 378

BLAST of Cp4.1LG16g06240 vs. ExPASy TrEMBL
Match: A0A1S3CKQ2 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502070 PE=4 SV=1)

HSP 1 Score: 683 bits (1763), Expect = 6.28e-247
Identity = 338/382 (88.48%), Postives = 359/382 (93.98%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRC+RFVRAW +D+ITCP+CDGGFVE IETASSLPA +LHRRLSP+AIHT
Sbjct: 1   MSTGTASYWCYRCSRFVRAWAQDSITCPYCDGGFVEAIETASSLPAPNLHRRLSPTAIHT 60

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           +DQD F + RLS+RRSRRR G+R+TFNP+VV+RGSAD GDVVGGG+RNSFDIYYDDGAGS
Sbjct: 61  VDQDSFQSPRLSTRRSRRRFGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 120

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEING GRSENPPASKAAVESMPTIEILESHV 
Sbjct: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 180

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSER SPAGGV
Sbjct: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERVSPAGGV 240

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANG PRRVSW
Sbjct: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGVPRRVSW 300

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
            SRRSRGRESRGIGRTFRNIFSFFGR RSS+SSS + SNESESVSRSHSHS     SSVF
Sbjct: 301 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHS----SSSVF 360

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           +RYLRRQNRAW +ENQ++N RW
Sbjct: 361 SRYLRRQNRAWVLENQNENGRW 378

BLAST of Cp4.1LG16g06240 vs. ExPASy TrEMBL
Match: A0A0A0KE74 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G190250 PE=4 SV=1)

HSP 1 Score: 685 bits (1767), Expect = 6.36e-247
Identity = 339/382 (88.74%), Postives = 359/382 (93.98%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHT 60
           M+TGTASYWCYRCNRFVRAW +D+ITCP+CDGGFVE IETASSLP ++LHRRLSPSAIHT
Sbjct: 39  MSTGTASYWCYRCNRFVRAWAQDSITCPYCDGGFVEAIETASSLPPSNLHRRLSPSAIHT 98

Query: 61  LDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDGAGS 120
           LDQD F + RLS+RRSRRRLG+R+TFNP+VV+RGSAD GDVVGGG+RNSFDIYYDDGAGS
Sbjct: 99  LDQDSFQSPRLSTRRSRRRLGDRSTFNPVVVLRGSADAGDVVGGGERNSFDIYYDDGAGS 158

Query: 121 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILESHVG 180
           GLRPVPATMSEFLMGTGFDRLLEQLAQLEING GRSENPPASKAAVESMPTIEILESHV 
Sbjct: 159 GLRPVPATMSEFLMGTGFDRLLEQLAQLEINGFGRSENPPASKAAVESMPTIEILESHVD 218

Query: 181 SDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERESPAGGV 240
           SDSHCAVCKEAFEIGTEAREMPCKHIYHS+CIIPWLSMRNSCPVCRHELPSER SPAGGV
Sbjct: 219 SDSHCAVCKEAFEIGTEAREMPCKHIYHSECIIPWLSMRNSCPVCRHELPSERVSPAGGV 278

Query: 241 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNANGAPRRVSW 300
           SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFN NG PRRVSW
Sbjct: 279 SDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGGFNGNGVPRRVSW 338

Query: 301 GSRRSRGRESRGIGRTFRNIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVF 360
            SRRSRGRESRGIGRTFRNIFSFFGR RSS+SSS + SNESESVSRSHSHS     SSVF
Sbjct: 339 ASRRSRGRESRGIGRTFRNIFSFFGRFRSSSSSSSSPSNESESVSRSHSHS----SSSVF 398

Query: 361 NRYLRRQNRAWAMENQSDNDRW 382
           +RYLRRQNRAW +ENQ++N RW
Sbjct: 399 SRYLRRQNRAWVLENQNENGRW 416

BLAST of Cp4.1LG16g06240 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 322.8 bits (826), Expect = 3.8e-88
Identity = 188/395 (47.59%), Postives = 247/395 (62.53%), Query Frame = 0

Query: 5   TASYWCYRCNRFVRAWVED------TITCPHCDGGFVETIETASSLPAADLHRRLSPSAI 64
           TASYWCY C RFV  W +       ++ CPHCDGGF+E I  +SS  A +L         
Sbjct: 15  TASYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSS-AATEL--------- 74

Query: 65  HTLDQDQFPTSRLSSRRS---RRRLGERTTFNPIVVVRGSA----DGGDVVGGGDRNSFD 124
            T+       S  ++RRS   RRR G R +FNP++V++G A    +G +     DR +F+
Sbjct: 75  -TIPASTEVRSINNNRRSVIRRRRSGRRPSFNPVIVLQGGAGEREEGEEGDAARDRRAFE 134

Query: 125 IYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN--GLGRSENPPASKAAVESM 184
            YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E +  G+GRS NPPASK+A+ES+
Sbjct: 135 FYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKSAIESL 194

Query: 185 PTIEILESHVGSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHEL 244
           P +EI + H+GS+++CAVC E FE  TEAREMPCKH++H DCI+PWLS+RNSCPVCR EL
Sbjct: 195 PRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFEL 254

Query: 245 PSERESPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMDGG- 304
           PSE        S+   ++  VG+TIWRLPGGGFAVGRF+     GER  P V TEMDGG 
Sbjct: 255 PSEPNRR----SNNNEEDNAVGMTIWRLPGGGFAVGRFNAAMRDGERVLPVVLTEMDGGG 314

Query: 305 -FNANGAPRRVSW-GSRRSRGRESRGIG----------RTFRNIFSFFGRL---RSSNSS 364
             N+   PRR+SW  S  +   +S G G          R  R + S   R+   RSS+SS
Sbjct: 315 IGNSQDGPRRISWVRSHGTLESDSNGTGSGSGSGGRLRRMVRGMVSLMRRVRPNRSSSSS 374

Query: 365 SPAVSNESESVSRSHSHSHS--HSGSSVFNRYLRR 367
           S A+S  S+++  +           +SV  RY RR
Sbjct: 375 SAAISTSSDNLDLNIEVESRVLDRSNSVMRRYFRR 394

BLAST of Cp4.1LG16g06240 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 312.0 bits (798), Expect = 6.6e-85
Identity = 176/378 (46.56%), Postives = 238/378 (62.96%), Query Frame = 0

Query: 3   TGTASYWCYRCNRFVRAWVEDT----ITCPHCDGGFVETIETASSLPAADLHRRLSPSAI 62
           T T SYWCY C RF+  W +      + CP+C+GGF+E IE +S+   A +    S   +
Sbjct: 26  TLTTSYWCYSCTRFISVWEDQDANAGVLCPYCNGGFIEEIEDSSNSTVAAI--PASTPEV 85

Query: 63  HTLDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGSADGG------DVVGGG---DRNS 122
            ++++    T R   R  RRR   RT+FNP++V+ G   GG      +  G G   +R +
Sbjct: 86  RSVEE----THRSIIR--RRRSNRRTSFNPVIVLHGGGGGGAGERVENEEGDGATRERRA 145

Query: 123 FDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEI--NGLGRSENPPASKAAVE 182
           ++ YYDDG+GSGLRP+P ++SE LMG+GF+RLLEQL+Q+E   NG+GRS NPPASK+A+E
Sbjct: 146 YEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASGNGIGRSGNPPASKSAIE 205

Query: 183 SMPTIEILESHVGSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRH 242
           S+P +EI + H  ++++CAVC E FE G E REMPCKHI+H DCI+PWLS+RNSCPVCR 
Sbjct: 206 SLPRVEISDCHTKAEANCAVCTEVFEAGIEGREMPCKHIFHGDCIVPWLSIRNSCPVCRF 265

Query: 243 ELPSERESPAGGVSDRVVDEETVGLTIWRLPGGGFAVGRFSGGRLAGERDPPAVYTEMD- 302
           ELPS+       +     +E  VG+TIWRLPGGGFAVGRF+ G   GER  P V TEMD 
Sbjct: 266 ELPSD------PIQRSNEEEHAVGMTIWRLPGGGFAVGRFNAGVREGERILPVVLTEMDG 325

Query: 303 GGFNANGAPRRVSW-------GSRRSRGRESRG--IGRTFRNIFSFFGRLRSS--NSSSP 354
           GG  +N  PRR+SW          R+ GR   G  + R  R + SF  R+R S  +S+S 
Sbjct: 326 GGLGSNEGPRRISWVRAHETPEMSRNGGRSGNGGRLRRAVRGMVSFMRRVRPSRGSSNSN 385

BLAST of Cp4.1LG16g06240 vs. TAIR 10
Match: AT2G39720.1 (RING-H2 finger C2A )

HSP 1 Score: 302.4 bits (773), Expect = 5.3e-82
Identity = 189/410 (46.10%), Postives = 246/410 (60.00%), Query Frame = 0

Query: 1   MATGTASYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAAD--------LHR- 60
           MA+G  SYWCY C+RFV  WV D+I+CP CDGGF+E I+       +D         HR 
Sbjct: 1   MASG--SYWCYSCSRFV--WVSDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRS 60

Query: 61  ----------RLSPSAIHTLDQDQFPTSRLSSRRSRRRLGERTTFNPIVVVRGS--ADGG 120
                       +PSA  ++  D  PT  + +R    R     + NP++V+RGS  A   
Sbjct: 61  PTRFPPPSSSSSTPSA--SMHADNSPTPTIVTRTRSNR-----SPNPVIVLRGSAAAPSS 120

Query: 121 DVVGGG-DRNSFDIYYDDGAGSGLRPVPATMSEFLMGTGFDRLLEQLAQLEIN---GLGR 180
           DVV  G DR++F +YYDDG  SGLRP+P +M+EFL+G+GFDRLL+Q++Q+E+N    L  
Sbjct: 121 DVVSEGLDRSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRS 180

Query: 181 SENPPASKAAVESMPTIEILESHVGSD--SHCAVCKEAFEIGTEAREMPCKHIYHSDCII 240
            E+PPASK+A+E++P IEI  +H+ SD  SHCAVCKE F + + AREMPC HIYH DCI+
Sbjct: 181 CEHPPASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCIL 240

Query: 241 PWLSMRNSCPVCRHELPSERESPAGGVSDRVV-------DEETVGLTIWRLPGGGFAVGR 300
           PWL++RNSCPVCRHELP+E  +   G +   V       ++   GLTIWRLPGGGFAVGR
Sbjct: 241 PWLAIRNSCPVCRHELPAEDLTDGTGAALTAVTATAEEEEDSAAGLTIWRLPGGGFAVGR 300

Query: 301 FSGGRLAGERDPPAVYTEMDGG-FNANGAPRRVSWGSRR-------SRGRESRGIGRTFR 360
             GG   G+R  P VYTE+DGG       PRRV+WGSRR       SR R     GR  R
Sbjct: 301 IPGGWRGGDRMMPVVYTEVDGGRLGDERLPRRVAWGSRRGGRDGGGSRERGGGFAGRIMR 360

Query: 361 NIFSFFGRLRSSNSSSPAVSNESESVSRSHSHSHSHSGSSVFNRYLRRQN 369
            +F  F     S +++ A S+ S S  R    + S S  S  +   RR+N
Sbjct: 361 -LFGCFSGSSGSIAAAAAASSGSGSRIRVTRRTRSFSMFSTASSSSRRRN 398

BLAST of Cp4.1LG16g06240 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 7.2e-39
Identity = 90/240 (37.50%), Postives = 132/240 (55.00%), Query Frame = 0

Query: 7   SYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHTLDQDQF 66
           ++WC+RC R V     D + C +C GGFVE I+   S    D+ R  +   +       F
Sbjct: 7   THWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAF--SAF 66

Query: 67  PTSRLSSRRSRRRLGER---------TTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDG 126
             SRL+ R   R +  R         +   P+++  G A     + GGD +S + +  +G
Sbjct: 67  MRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVEAFV-NG 126

Query: 127 AGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILE 186
           A  G+     T   ++  G G + L+EQL+    +G      PPA K++++++PTI+I +
Sbjct: 127 AAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALPTIKITQ 186

Query: 187 SHV-GSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERES 236
            H+  SDSHC VCK+ FE+ +EA++MPC HIYHSDCI+PWL   NSCPVCR ELPS   S
Sbjct: 187 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSS 236

BLAST of Cp4.1LG16g06240 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 159.1 bits (401), Expect = 7.2e-39
Identity = 90/240 (37.50%), Postives = 132/240 (55.00%), Query Frame = 0

Query: 7   SYWCYRCNRFVRAWVEDTITCPHCDGGFVETIETASSLPAADLHRRLSPSAIHTLDQDQF 66
           ++WC+RC R V     D + C +C GGFVE I+   S    D+ R  +   +       F
Sbjct: 7   THWCHRCQRAVWLRARDAV-CSYCGGGFVEEIDIGPSRAHRDVERDPTFDLMEAF--SAF 66

Query: 67  PTSRLSSRRSRRRLGER---------TTFNPIVVVRGSADGGDVVGGGDRNSFDIYYDDG 126
             SRL+ R   R +  R         +   P+++  G A     + GGD +S + +  +G
Sbjct: 67  MRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFR--LAGGDNSSVEAFV-NG 126

Query: 127 AGSGLRPVPAT-MSEFLMGTGFDRLLEQLAQLEINGLGRSENPPASKAAVESMPTIEILE 186
           A  G+     T   ++  G G + L+EQL+    +G      PPA K++++++PTI+I +
Sbjct: 127 AAPGIGIARGTNAGDYFFGPGLEELIEQLS----SGTHHRGPPPAPKSSIDALPTIKITQ 186

Query: 187 SHV-GSDSHCAVCKEAFEIGTEAREMPCKHIYHSDCIIPWLSMRNSCPVCRHELPSERES 236
            H+  SDSHC VCK+ FE+ +EA++MPC HIYHSDCI+PWL   NSCPVCR ELPS   S
Sbjct: 187 KHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSS 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q940T55.3e-8747.59E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
Q9SNB69.3e-8446.56E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
O222837.4e-8146.10Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q94AK41.0e-3737.50E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221972.1e-3537.50Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Match NameE-valueIdentityDescription
XP_023512263.14.44e-279100.00E3 ubiquitin-protein ligase RDUF2-like [Cucurbita pepo subsp. pepo][more]
XP_022986887.11.05e-27899.48E3 ubiquitin-protein ligase RDUF2-like [Cucurbita maxima][more]
KAG6570690.11.04e-27498.44E3 ubiquitin-protein ligase RDUF2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022944700.19.96e-27397.92E3 ubiquitin-protein ligase RDUF2-like [Cucurbita moschata][more]
KAE8646961.13.19e-24788.74hypothetical protein Csa_020907 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A6J1J8T85.08e-27999.48RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111484489 P... [more]
A0A6J1FYT84.82e-27397.92RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111449083... [more]
A0A5A7V7N56.28e-24788.48RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CKQ26.28e-24788.48RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103502070 PE=4 ... [more]
A0A0A0KE746.36e-24788.74RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G190250 PE... [more]
Match NameE-valueIdentityDescription
AT5G59550.13.8e-8847.59zinc finger (C3HC4-type RING finger) family protein [more]
AT3G46620.16.6e-8546.56zinc finger (C3HC4-type RING finger) family protein [more]
AT2G39720.15.3e-8246.10RING-H2 finger C2A [more]
AT3G56580.27.2e-3937.50RING/U-box superfamily protein [more]
AT3G56580.17.2e-3937.50RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 185..225
e-value: 3.0E-7
score: 40.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 184..226
e-value: 1.2E-10
score: 41.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 185..226
score: 12.135206
IPR010543Domain of unknown function DUF1117PFAMPF06547DUF1117coord: 250..361
e-value: 4.1E-39
score: 133.6
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 7..38
e-value: 5.0E-14
score: 52.3
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 151..232
e-value: 2.8E-21
score: 77.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 328..359
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 5..313
NoneNo IPR availablePANTHERPTHR15710:SF24OS05G0110000 PROTEINcoord: 5..313
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 185..226
e-value: 3.4099E-24
score: 91.9956
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 165..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g06240.1Cp4.1LG16g06240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity
molecular_function GO:0061630 ubiquitin protein ligase activity