Cp4.1LG16g05000 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g05000 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 204.9 bits (520), Expect = 1.1e-51
Identity = 129/279 (46.24%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           K+ G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G  
Sbjct: 12  KKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGAL 71

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEK----------------KSKKPEDEKPKDEEAP 120
           D   LREKL  KTKKK+D+VS + KKEK                + KKP++   K +E P
Sbjct: 72  DPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETP 131

Query: 121 VTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKL 180
           VTTA LK+  HCQGC+ +I K V++TKGV  + M+++ +L+TVKG MDVK L+  L +KL
Sbjct: 132 VTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKL 191

Query: 181 KRKVAVVVPKKDKDDGDG---ADKNKSGG------EVTQENGGGETDGDRLDYMAV-PAP 240
           KR V +V PKK+KD  +G    +K K GG      E T   GGGE   + ++YMA  PA 
Sbjct: 192 KRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAAQPAY 251

Query: 241 GYEYGFGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 254
           GY Y  G  YGY     +  H P  Q+FSDENPNAC VM
Sbjct: 252 GYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVM 283

BLAST of Cp4.1LG16g05000 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-29
Identity = 117/335 (34.93%), Postives = 151/335 (45.07%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLREKLAGKTKKKI 77
           V+ +DMHCEGCA K++R V+   GV+ V AD   NKL V+GK D   L+EKL  KTK+K+
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKV 112

Query: 78  DIVSTEGKKEKKSKKPEDEKPKD----EEAPVTTA---------TLKVELHCQGCVERIY 137
            + +   K E        EK  D    E AP   A          LK+ LHC+GC+++I 
Sbjct: 113 VLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIK 172

Query: 138 KVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG---- 197
           K++ + KGVE +A++   D+VTVKG +DVK L+  L  KLKR V  +VP K KDDG    
Sbjct: 173 KIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAK-KDDGAAEN 232

Query: 198 ---------------------------DGADKNK---SGGEVTQENG-GGET-----DG- 254
                                      DG +K K    GGE  +E G GGE      DG 
Sbjct: 233 KKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGG 292

BLAST of Cp4.1LG16g05000 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 5.7e-29
Identity = 94/251 (37.45%), Postives = 138/251 (54.98%), Query Frame = 0

Query: 10  KEEDSTTA----VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIG-KFDAST 69
           K++++TT+    V  ID HC+GC  ++ R  R+++GVE VRAD ++NKLT+IG   D   
Sbjct: 20  KKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVK 79

Query: 70  LREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEE-APVTTATLKVELHCQGCVERI 129
           + EKL  K+KKK++++S + KK+ K    +    K +    VTT  LKV   C GC++RI
Sbjct: 80  IAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTTVVLKVNCSCDGCIKRI 139

Query: 130 YKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVV-VPKKDKDDGDG 189
            K VS TKGV  + M+++ + VTV G MD+K++  NL+ KLK+ V VV   KK K D D 
Sbjct: 140 QKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKKKKDKDN 199

Query: 190 ADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQMF 249
           A+ N   G   Q                 P  G  +G G  Y +  G       P +  F
Sbjct: 200 AEVNTKVGSPCQ-----------------PGNGCTHGIG-PYRFMEG-------PMTGFF 245

Query: 250 SDENPNACTVM 254
           S+E+ + C+VM
Sbjct: 260 SEEDQSYCSVM 245

BLAST of Cp4.1LG16g05000 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 2.2e-28
Identity = 118/379 (31.13%), Postives = 160/379 (42.22%), Query Frame = 0

Query: 7   GSDKEEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDAST 66
           G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V+ D+++NKLTVIG  D   
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVE 73

Query: 67  LREKLAGKTKKKIDIVST-----------EGKKEKK-----SKKPEDEKPKDEEAP---- 126
           +R+K+A K K+ +++VST            G  EKK      +KP ++KP   E P    
Sbjct: 74  VRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKK 133

Query: 127 -------------------VTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLV 186
                               +T  LK +LHC+GC  +I ++V++ KGV  +A++   DLV
Sbjct: 134 EEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLV 193

Query: 187 TVKGKMDVKALIHNLEDKLKRKVAVVVPKKD-------------------------KDDG 246
            VKG +DVK L   L +KLKR V VV  KKD                         K+  
Sbjct: 194 IVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIK 253

Query: 247 D-GADKNKSGGEVTQE--------NGGGETDGDRLD--------YMAVPAPGYEYGFGYG 254
           D G  K   GGE  +E         GGG  DG  +D        Y   P P Y Y  G  
Sbjct: 254 DVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQV 313

BLAST of Cp4.1LG16g05000 vs. ExPASy Swiss-Prot
Match: Q9FLU5 (Heavy metal-associated isoprenylated plant protein 9 OS=Arabidopsis thaliana OX=3702 GN=HIPP09 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.4e-19
Identity = 77/263 (29.28%), Postives = 121/263 (46.01%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLREKLAGKTKKKI 77
           +  +D+HC GCA K+ R + +I+GVE V  D   N++T+ G  D   +  K+  KTK+  
Sbjct: 59  ILYVDLHCVGCAKKIERSILKIRGVEEVVMDMNENQVTIKGVLDPQAVCNKIKKKTKRMA 118

Query: 78  DIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMA 137
            ++S     E +   P           +TT  L V +HCQ C +++ K + + +GV+   
Sbjct: 119 KVLSPLPAAEGEPLPP--IITSQVSGGLTTVELSVNMHCQACADQLKKKILKMRGVQTTV 178

Query: 138 MERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVV-------------VPKKDKDDGDGAD 197
            E     V V G MD + L+  +  + K++  +V               +K ++ G+G +
Sbjct: 179 TEHTTGKVIVTGTMDAEKLVDYVYRRTKKQARIVPQPDPEPEAPAAAQEEKKEESGEGNE 238

Query: 198 K-----------NKSGGEVTQENGGGE----TDGDRLDYMAVPAPGYEYGFGYGYGYGHG 253
           K            K  GE   E GGGE    T+ +R D   + A   E G      Y   
Sbjct: 239 KPPETGEEKEEEKKKEGEENGEEGGGEEAAATEEERRD-NEMTAMAQEEGMKRMMYYYQP 298

BLAST of Cp4.1LG16g05000 vs. NCBI nr
Match: XP_023513163.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494 bits (1273), Expect = 3.75e-176
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF
Sbjct: 4   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCV 120
           DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCV
Sbjct: 64  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCV 123

Query: 121 ERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG 180
           ERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG
Sbjct: 124 ERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG 183

Query: 181 DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQ 240
           DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQ
Sbjct: 184 DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQ 243

Query: 241 MFSDENPNACTVM 253
           MFSDENPNACTVM
Sbjct: 244 MFSDENPNACTVM 256

BLAST of Cp4.1LG16g05000 vs. NCBI nr
Match: XP_022944012.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita moschata])

HSP 1 Score: 465 bits (1196), Expect = 2.55e-164
Identity = 242/259 (93.44%), Postives = 247/259 (95.37%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           KQNG+HGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF
Sbjct: 4   KQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKP----EDEKPKDEEAPVTTATLKVELHC 120
           DASTLREKLA KT KKIDIVS+EGKKEKKSKKP    EDEKPKD+E PVTTATLKVELHC
Sbjct: 64  DASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQ DLVTVKGKMDVKAL+HNLEDKLKRKVAVVVPKKD
Sbjct: 124 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 183

Query: 181 KDDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYG--HGGFVGE 240
            DDGDG DKNKSGGEVTQE+GGGETDGDRLDYMAVPAPGYEYGFGYGYGYG  HGGFVGE
Sbjct: 184 NDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 253
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 262

BLAST of Cp4.1LG16g05000 vs. NCBI nr
Match: XP_022985576.1 (heavy metal-associated isoprenylated plant protein 3 [Cucurbita maxima])

HSP 1 Score: 464 bits (1195), Expect = 2.91e-164
Identity = 237/253 (93.68%), Postives = 244/253 (96.44%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           K+NG+HGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF
Sbjct: 4   KKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCV 120
           DAS+LREKLA KTKKKIDIVS EGKKEK SKKPEDEKPKDEEAP TTATLKVELHCQGC+
Sbjct: 64  DASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQGCI 123

Query: 121 ERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG 180
           ERIYKVVSRTKGVEDMAME Q DLVTVKGKMDVKALIHNLE+KLKRKVAVVVPKKDKDDG
Sbjct: 124 ERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDKDDG 183

Query: 181 DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQ 240
           DG DKNKSGGEV Q++GGGETDGDRLDYMAVPAPGYEYGFGYGY YGHGGFVGEHLPSSQ
Sbjct: 184 DGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLPSSQ 243

Query: 241 MFSDENPNACTVM 253
           MFSDENPNACTVM
Sbjct: 244 MFSDENPNACTVM 256

BLAST of Cp4.1LG16g05000 vs. NCBI nr
Match: KAG7010652.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460 bits (1184), Expect = 1.54e-162
Identity = 239/259 (92.28%), Postives = 244/259 (94.21%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           KQN  HGSDK+EDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVR DWEANKLTVIGKF
Sbjct: 1   KQNSGHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRVDWEANKLTVIGKF 60

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKP----EDEKPKDEEAPVTTATLKVELHC 120
           DASTLREKLA KTKKKIDIVS+EGKKEKKSKKP    EDEKPKD+E PVTTATLKVELHC
Sbjct: 61  DASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 120

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQ DLVTVKGKMDVKAL+HNLEDKLKRKVAVVVPKKD
Sbjct: 121 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 180

Query: 181 KDDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYG--HGGFVGE 240
            DDGDG DKNKSGGEV QENGGGETDGDRLDYMAVPAPGYEYG+GYGYGYG  HGGFVGE
Sbjct: 181 SDDGDGGDKNKSGGEVAQENGGGETDGDRLDYMAVPAPGYEYGYGYGYGYGYGHGGFVGE 240

Query: 241 HLPSSQMFSDENPNACTVM 253
           HLPSSQMFSDENPNACTVM
Sbjct: 241 HLPSSQMFSDENPNACTVM 259

BLAST of Cp4.1LG16g05000 vs. NCBI nr
Match: KAG6570811.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 385 bits (988), Expect = 7.90e-132
Identity = 204/220 (92.73%), Postives = 209/220 (95.00%), Query Frame = 0

Query: 40  QGVERVRADWEANKLTVIGKFDASTLREKLAGKTKKKIDIVSTEGKKEKKSKKP----ED 99
           +GVERVRADWEANKLTVIGKFDASTLREKLA KTKKKIDIVS+EGKKEKKSKKP    ED
Sbjct: 96  EGVERVRADWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEEAED 155

Query: 100 EKPKDEEAPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKA 159
           EKPKD+E PVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQ DLVTVKGKMDVKA
Sbjct: 156 EKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKA 215

Query: 160 LIHNLEDKLKRKVAVVVPKKDKDDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPG 219
           L+HNLEDKLKRKVAVVVPKKD DDGDG DKNKSGGEVTQE+GGGETDGDRLDYMAVPAPG
Sbjct: 216 LVHNLEDKLKRKVAVVVPKKDSDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPG 275

Query: 220 YEYGFGYGYGYG--HGGFVGEHLPSSQMFSDENPNACTVM 253
           YEYGFGYGYGYG  HGGFVGEHLPSSQMFSDENPNACTVM
Sbjct: 276 YEYGFGYGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 315

BLAST of Cp4.1LG16g05000 vs. ExPASy TrEMBL
Match: A0A6J1FUL5 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111448569 PE=4 SV=1)

HSP 1 Score: 465 bits (1196), Expect = 1.23e-164
Identity = 242/259 (93.44%), Postives = 247/259 (95.37%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           KQNG+HGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF
Sbjct: 4   KQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKP----EDEKPKDEEAPVTTATLKVELHC 120
           DASTLREKLA KT KKIDIVS+EGKKEKKSKKP    EDEKPKD+E PVTTATLKVELHC
Sbjct: 64  DASTLREKLADKTNKKIDIVSSEGKKEKKSKKPDEEAEDEKPKDKEIPVTTATLKVELHC 123

Query: 121 QGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKD 180
           QGCVERIYKVVSRTKGVEDMAMERQ DLVTVKGKMDVKAL+HNLEDKLKRKVAVVVPKKD
Sbjct: 124 QGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKMDVKALVHNLEDKLKRKVAVVVPKKD 183

Query: 181 KDDGDGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYG--HGGFVGE 240
            DDGDG DKNKSGGEVTQE+GGGETDGDRLDYMAVPAPGYEYGFGYGYGYG  HGGFVGE
Sbjct: 184 NDDGDGGDKNKSGGEVTQEDGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGFVGE 243

Query: 241 HLPSSQMFSDENPNACTVM 253
           HLPSSQMFSDENPNACTVM
Sbjct: 244 HLPSSQMFSDENPNACTVM 262

BLAST of Cp4.1LG16g05000 vs. ExPASy TrEMBL
Match: A0A6J1J8L7 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111483606 PE=4 SV=1)

HSP 1 Score: 464 bits (1195), Expect = 1.41e-164
Identity = 237/253 (93.68%), Postives = 244/253 (96.44%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           K+NG+HGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF
Sbjct: 4   KKNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 63

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEEAPVTTATLKVELHCQGCV 120
           DAS+LREKLA KTKKKIDIVS EGKKEK SKKPEDEKPKDEEAP TTATLKVELHCQGC+
Sbjct: 64  DASSLREKLADKTKKKIDIVSLEGKKEKGSKKPEDEKPKDEEAPATTATLKVELHCQGCI 123

Query: 121 ERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG 180
           ERIYKVVSRTKGVEDMAME Q DLVTVKGKMDVKALIHNLE+KLKRKVAVVVPKKDKDDG
Sbjct: 124 ERIYKVVSRTKGVEDMAMETQKDLVTVKGKMDVKALIHNLEEKLKRKVAVVVPKKDKDDG 183

Query: 181 DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQ 240
           DG DKNKSGGEV Q++GGGETDGDRLDYMAVPAPGYEYGFGYGY YGHGGFVGEHLPSSQ
Sbjct: 184 DGGDKNKSGGEVAQQDGGGETDGDRLDYMAVPAPGYEYGFGYGYVYGHGGFVGEHLPSSQ 243

Query: 241 MFSDENPNACTVM 253
           MFSDENPNACTVM
Sbjct: 244 MFSDENPNACTVM 256

BLAST of Cp4.1LG16g05000 vs. ExPASy TrEMBL
Match: A0A1S3CP34 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103502993 PE=4 SV=1)

HSP 1 Score: 363 bits (932), Expect = 2.37e-124
Identity = 199/268 (74.25%), Postives = 222/268 (82.84%), Query Frame = 0

Query: 7   GSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLR 66
           G  K +D TTAVF ID+HCEGCANKLR+ VR+I GV RVRADWEANKLTVIGKFDA+ LR
Sbjct: 2   GEKKNDDITTAVFKIDIHCEGCANKLRKSVREIPGVARVRADWEANKLTVIGKFDAAKLR 61

Query: 67  EKLAGKTKKKIDIVSTEGKKEKKS-KKPEDEKP----------KDEEAPVTTATLKVELH 126
           + LA KTKKKIDIVS+EGKKEK+S KK +DEKP          KD+E PVTTATLKVELH
Sbjct: 62  DNLADKTKKKIDIVSSEGKKEKESAKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 127 CQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKK 186
           CQGC+E+IYKVVSRTKGVEDMA+ERQ DLV VKGKMDVKALI NLE+KLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 187 DKDDG----DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGF 246
           DKDDG    DG DKNK+GGEV QE  GG  +G+RLDY+AVP PGY YG+GYGYGYG+GGF
Sbjct: 182 DKDDGAKGGDGGDKNKTGGEVAQE--GGAMEGNRLDYVAVPVPGYGYGYGYGYGYGNGGF 241

Query: 247 VGEHLP------SSQMFSDENPNACTVM 253
           VG+HLP      S QMFSDENPNAC++M
Sbjct: 242 VGQHLPQPQPLLSPQMFSDENPNACSLM 267

BLAST of Cp4.1LG16g05000 vs. ExPASy TrEMBL
Match: A0A0A0KDD7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV=1)

HSP 1 Score: 357 bits (915), Expect = 9.15e-122
Identity = 196/268 (73.13%), Postives = 219/268 (81.72%), Query Frame = 0

Query: 7   GSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLR 66
           G  K +D+TTAVF ID+HCEGCANKLRR VRQI GV RVRADWEANKLTVIGKFD + LR
Sbjct: 2   GEKKNDDTTTAVFKIDIHCEGCANKLRRSVRQIPGVSRVRADWEANKLTVIGKFDPAKLR 61

Query: 67  EKLAGKTKKKIDIVSTEGKKEKKS-KKPEDEKP----------KDEEAPVTTATLKVELH 126
           + LA K  KKIDIVS+E KKEK+S KK +DEKP          KD+E PVTTATLKVELH
Sbjct: 62  DYLADKETKKIDIVSSESKKEKESTKKQDDEKPDKKTEDKKQPKDKEIPVTTATLKVELH 121

Query: 127 CQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKK 186
           CQGC+E+IYKVVSRTKGVEDMA+ERQ DLV VKGKMDVKALI NLE+KLKRKVAVVVPKK
Sbjct: 122 CQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKK 181

Query: 187 DKDDG----DGADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGF 246
           DKD+G    DG DKNK+GGEV Q  GGG  +G+RLDY+AVP PGY YG+GYGYGYG+GGF
Sbjct: 182 DKDEGAKGGDGGDKNKTGGEVAQ--GGGAMEGNRLDYVAVPVPGYGYGYGYGYGYGNGGF 241

Query: 247 VGEHLP------SSQMFSDENPNACTVM 253
           VG+H+P      S QMFSDENPNAC+VM
Sbjct: 242 VGQHMPPAQPLISPQMFSDENPNACSVM 267

BLAST of Cp4.1LG16g05000 vs. ExPASy TrEMBL
Match: A0A6J1K7Z0 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111491473 PE=4 SV=1)

HSP 1 Score: 351 bits (900), Expect = 9.34e-120
Identity = 190/257 (73.93%), Postives = 211/257 (82.10%), Query Frame = 0

Query: 7   GSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLR 66
           G  K ED+TTAVF IDMHCEGCANK+R+CVR+I+GVER RADWEANKLTVIGKFD + LR
Sbjct: 2   GEKKNEDTTTAVFKIDMHCEGCANKIRKCVREIEGVERARADWEANKLTVIGKFDPAKLR 61

Query: 67  EKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKP-----KDEEAPVTTATLKVELHCQGCVE 126
           EKLA KTKKKIDIVS+E KKEK+SKKP+D+KP     KD+E PVTT TLKVELHCQGC+E
Sbjct: 62  EKLADKTKKKIDIVSSENKKEKESKKPDDKKPEEKKSKDKETPVTTVTLKVELHCQGCIE 121

Query: 127 RIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG- 186
           +I+KVVSRTKGVEDMA+ERQ DLV VKGKMDVKALI NLE+KL+RKVAVVVPKKDKDDG 
Sbjct: 122 KIHKVVSRTKGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLRRKVAVVVPKKDKDDGA 181

Query: 187 ---DGADKNKSGGEVTQE-NGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHL 246
              D +DKNKS G+  Q  NGGG  DG RLD+MAVP  GY YG+GYG         GEHL
Sbjct: 182 KGGDSSDKNKSSGDAAQPANGGGGGDGHRLDHMAVPVSGYGYGYGYG---------GEHL 241

Query: 247 PSSQMFSDENPNACTVM 253
           PS QMFSDENPNAC VM
Sbjct: 242 PSVQMFSDENPNACLVM 249

BLAST of Cp4.1LG16g05000 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 204.9 bits (520), Expect = 7.6e-53
Identity = 129/279 (46.24%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           K+ G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G  
Sbjct: 12  KKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGAL 71

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEK----------------KSKKPEDEKPKDEEAP 120
           D   LREKL  KTKKK+D+VS + KKEK                + KKP++   K +E P
Sbjct: 72  DPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETP 131

Query: 121 VTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKL 180
           VTTA LK+  HCQGC+ +I K V++TKGV  + M+++ +L+TVKG MDVK L+  L +KL
Sbjct: 132 VTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKL 191

Query: 181 KRKVAVVVPKKDKDDGDG---ADKNKSGG------EVTQENGGGETDGDRLDYMAV-PAP 240
           KR V +V PKK+KD  +G    +K K GG      E T   GGGE   + ++YMA  PA 
Sbjct: 192 KRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAAQPAY 251

Query: 241 GYEYGFGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 254
           GY Y  G  YGY     +  H P  Q+FSDENPNAC VM
Sbjct: 252 GYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVM 283

BLAST of Cp4.1LG16g05000 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 203.4 bits (516), Expect = 2.2e-52
Identity = 128/279 (45.88%), Postives = 168/279 (60.22%), Query Frame = 0

Query: 1   KQNGAHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKF 60
           K+ G +    E  S T V  +DMHCEGCA+++ +CVR  QGVE V+++    KLTV G  
Sbjct: 12  KKGGDNKKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQGVETVKSESATGKLTVTGAL 71

Query: 61  DASTLREKLAGKTKKKIDIVSTEGKKEK----------------KSKKPEDEKPKDEEAP 120
           D   LREKL  KTKKK+D+VS + KKEK                + KKP++   K +E P
Sbjct: 72  DPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKKSEEKKKPDNNDKKPKETP 131

Query: 121 VTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKL 180
           VTTA LK+  HCQGC+ +I K V++TKGV  + M+++ +L+TVKG MDVK L+  L +KL
Sbjct: 132 VTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLTVKGTMDVKKLVEILSEKL 191

Query: 181 KRKVAVVVPKKDKDDGDG---ADKNKSGG------EVTQENGGGETDGDRLDYMAV-PAP 240
           KR V +V PKK+KD  +G    +K K GG      E T   GGGE   + ++YMA  PA 
Sbjct: 192 KRAVEIVPPKKEKDKENGNENGEKKKGGGGDGGGKEKTGNKGGGE-GVNMMEYMAAQPAY 251

Query: 241 GYEYGFGYGYGYGHGGFVGEHLPSSQMFSDENPNACTVM 254
           GY Y  G  YGY     +  H P  Q+FSDENPNAC V+
Sbjct: 252 GYGYYPGGPYGYP----IQAHAP--QIFSDENPNACVVI 283

BLAST of Cp4.1LG16g05000 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 8.1e-31
Identity = 117/335 (34.93%), Postives = 151/335 (45.07%), Query Frame = 0

Query: 18  VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDASTLREKLAGKTKKKI 77
           V+ +DMHCEGCA K++R V+   GV+ V AD   NKL V+GK D   L+EKL  KTK+K+
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDGVKDVTADTGGNKLLVVGKIDPVKLQEKLEEKTKRKV 112

Query: 78  DIVSTEGKKEKKSKKPEDEKPKD----EEAPVTTA---------TLKVELHCQGCVERIY 137
            + +   K E        EK  D    E AP   A          LK+ LHC+GC+++I 
Sbjct: 113 VLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAPAAPKESVVPLKIRLHCEGCIQKIK 172

Query: 138 KVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVVVPKKDKDDG---- 197
           K++ + KGVE +A++   D+VTVKG +DVK L+  L  KLKR V  +VP K KDDG    
Sbjct: 173 KIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLLTKKLKRTVEPLVPAK-KDDGAAEN 232

Query: 198 ---------------------------DGADKNK---SGGEVTQENG-GGET-----DG- 254
                                      DG +K K    GGE  +E G GGE      DG 
Sbjct: 233 KKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEVGDGGEKKKEGGDGGEKKKEAGDGG 292

BLAST of Cp4.1LG16g05000 vs. TAIR 10
Match: AT2G28090.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 129.4 bits (324), Expect = 4.0e-30
Identity = 94/251 (37.45%), Postives = 138/251 (54.98%), Query Frame = 0

Query: 10  KEEDSTTA----VFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIG-KFDAST 69
           K++++TT+    V  ID HC+GC  ++ R  R+++GVE VRAD ++NKLT+IG   D   
Sbjct: 20  KKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEGVETVRADPDSNKLTLIGFIMDPVK 79

Query: 70  LREKLAGKTKKKIDIVSTEGKKEKKSKKPEDEKPKDEE-APVTTATLKVELHCQGCVERI 129
           + EKL  K+KKK++++S + KK+ K    +    K +    VTT  LKV   C GC++RI
Sbjct: 80  IAEKLQKKSKKKVELISPKPKKDTKENNEKKANDKTQTVVAVTTVVLKVNCSCDGCIKRI 139

Query: 130 YKVVSRTKGVEDMAMERQMDLVTVKGKMDVKALIHNLEDKLKRKVAVV-VPKKDKDDGDG 189
            K VS TKGV  + M+++ + VTV G MD+K++  NL+ KLK+ V VV   KK K D D 
Sbjct: 140 QKAVSTTKGVYQVKMDKEKETVTVMGTMDIKSVTDNLKRKLKKTVQVVPEKKKKKKDKDN 199

Query: 190 ADKNKSGGEVTQENGGGETDGDRLDYMAVPAPGYEYGFGYGYGYGHGGFVGEHLPSSQMF 249
           A+ N   G   Q                 P  G  +G G  Y +  G       P +  F
Sbjct: 200 AEVNTKVGSPCQ-----------------PGNGCTHGIG-PYRFMEG-------PMTGFF 245

Query: 250 SDENPNACTVM 254
           S+E+ + C+VM
Sbjct: 260 SEEDQSYCSVM 245

BLAST of Cp4.1LG16g05000 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.5e-29
Identity = 118/379 (31.13%), Postives = 160/379 (42.22%), Query Frame = 0

Query: 7   GSDKEEDS--TTAVFNIDMHCEGCANKLRRCVRQIQGVERVRADWEANKLTVIGKFDAST 66
           G  K  D   TT V  +DMHCEGC  K++R  +  +GVE V+ D+++NKLTVIG  D   
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVEDVKIDYKSNKLTVIGNVDPVE 73

Query: 67  LREKLAGKTKKKIDIVST-----------EGKKEKK-----SKKPEDEKPKDEEAP---- 126
           +R+K+A K K+ +++VST            G  EKK      +KP ++KP   E P    
Sbjct: 74  VRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPAAVEKPGEKK 133

Query: 127 -------------------VTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQMDLV 186
                               +T  LK +LHC+GC  +I ++V++ KGV  +A++   DLV
Sbjct: 134 EEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSVAIDSAKDLV 193

Query: 187 TVKGKMDVKALIHNLEDKLKRKVAVVVPKKD-------------------------KDDG 246
            VKG +DVK L   L +KLKR V VV  KKD                         K+  
Sbjct: 194 IVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDKVAGEKKEIK 253

Query: 247 D-GADKNKSGGEVTQE--------NGGGETDGDRLD--------YMAVPAPGYEYGFGYG 254
           D G  K   GGE  +E         GGG  DG  +D        Y   P P Y Y  G  
Sbjct: 254 DVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGYPPQPMYYYPEGQV 313