Cp4.1LG16g04550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG16g04550
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Auxin-responsive GH3 family protein
Location	Cp4.1LG16: 5814300 .. 5815842 (-)
RNA-Seq Expression	Cp4.1LG16g04550
Synteny	Cp4.1LG16g04550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTTCTTGCAGCTCTGGGACATCTGGTGGAGAGCCCAAGTTGATTCCAATGTATGAAGAAGAGTTCATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAACTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACTCCAACCAAAGCCTGTACTGTCAGTTGCTTTGTGGGCTGTGTCAGAACGACATAGTGTTTCGAGTTGGTGCTCTGTTTGCCTCAACCTTAATCCATGTCCTTAAATTCCTCGAGATCATTGGGTTGTTTTGGCTAGTGATATAAGAGCAGGAACCTTGAACACCAAAATTACAGACCCATCAGTGAGAGAAGCTGTTATGAACATTCTAAACCCCAATGCTCAACTTGCAGATTTTATTGAAGCTGAATGTAGCAAAGGAACATCAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGTCACAGTACCTTCCCAAGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGGTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTATACTTTAATCCCGACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTTGTCATCACTACTTATGCTGGCAAGTCTGTCTGTCTGTCTTCTTCTTTTCAATATTGTTCTTGGGAGTTCTGATTTAGTTCATGAATAAGTTTACTGTTGCAGGGCTCTACCGTTATTGCATGGGGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAAACGTTGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGCTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCTGACTACACTAGCCATGCGGATAGCTCCAAGTTTCCTGGACACTATGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTCGCCATTGAAGAGTCACTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACTTTTGAGAAACTCATGGCACAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATTCTACGCACATCATTGAGCTTTTGGACTCGAACGTTGTTACCAAATACTTCAGTAAAGAATGCCCAAAAGTGGATACCAACGCTCTGCACCGTCTTCTTTCCAAGTAG

mRNA sequence

ATGGTTTCTTGCAGCTCTGGGACATCTGGTGGAGAGCCCAAGTTGATTCCAATGTATGAAGAAGAGTTCATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAACTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACTCCAACCAAAGCCTGTACTGTCAGTTGCTTTGTGGGCTGTGTCAGAACGACATAGTGTTTCGAGTTGGAACCTTGAACACCAAAATTACAGACCCATCAGTGAGAGAAGCTGTTATGAACATTCTAAACCCCAATGCTCAACTTGCAGATTTTATTGAAGCTGAATGTAGCAAAGGAACATCAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGTCACAGTACCTTCCCAAGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGGTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTATACTTTAATCCCGACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTTGTCATCACTACTTATGCTGGCAAGTCTGTCTGGCTCTACCGTTATTGCATGGGGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAAACGTTGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGCTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCTGACTACACTAGCCATGCGGATAGCTCCAAGTTTCCTGGACACTATGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTCGCCATTGAAGAGTCACTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACTTTTGAGAAACTCATGGCACAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATTCTACGCACATCATTGAGCTTTTGGACTCGAACGTTGTTACCAAATACTTCAGTAAAGAATGCCCAAAAGTGGATACCAACGCTCTGCACCGTCTTCTTTCCAAGTAG

Coding sequence (CDS)

ATGGTTTCTTGCAGCTCTGGGACATCTGGTGGAGAGCCCAAGTTGATTCCAATGTATGAAGAAGAGTTCATGAGAAGGCTATCATTTTTTGACTATGTGATGCCAAGAATGAAGCAGCTGTTTCCAAATAGTTTGGTGGGCAAAGGCAAAGGAATGCACTTTCAGTTTGCAAAGGTTGAGTTAAAGACAAGATCTGGAATTGTTGCTCACACTGCAACTACTAGTTTGTGCAAGAGAGCAAACCTCAATAATACAAGTCCTAAAGACATCATTCTCTGCACAGACTCCAACCAAAGCCTGTACTGTCAGTTGCTTTGTGGGCTGTGTCAGAACGACATAGTGTTTCGAGTTGGAACCTTGAACACCAAAATTACAGACCCATCAGTGAGAGAAGCTGTTATGAACATTCTAAACCCCAATGCTCAACTTGCAGATTTTATTGAAGCTGAATGTAGCAAAGGAACATCAAGGCTATGGCCGAACGCCAAATACATTAATGCTATTGTGACAGGAAGCATGTCACAGTACCTTCCCAAGCTAAATTACTACAGTAATCACCTTCCTATTGTTTCTGACCATTATGGTGCTTCAGAATGCTTCCTTGGGTTAAACTTAGATCCATTGTGTAGCCCTGATCGAACCTCCTATACTTTAATCCCGACCATGGCATATTTCGAGTTCCTGCCGATCGACAGGACGAAATCCAGTAGAGAAGTAAGCCGAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACATGAATATGAGCTTGTCATCACTACTTATGCTGGCAAGTCTGTCTGGCTCTACCGTTATTGCATGGGGGACATTCTTCGTGTGACAGGATTTAAGAACAAAGCTCCAAATTTTAGTTTTGTATGCAGGAAAAACGTTGTTTTGAGCCTTGATATTGAGAAAACAGATGAAGCAGAGCTTCATGATGCTGTACAAGAAGCAGCTCGTCTTTTGAGCCCATTTGGGGCGGCCATTGCTGACTACACTAGCCATGCGGATAGCTCCAAGTTTCCTGGACACTATGTGTTGTACTGGGAAATTCACGTTAAGGGGGGAATTTCTATTCCATCCTCAATCTATGAGGATTGCTGCTTCGCCATTGAAGAGTCACTTAATGGACCGTACCGCTATCTACGATCTCATGAGAAGTGTATCAATCCATTGGAGATAAAGATTGTAAAGGTTGGAACTTTTGAGAAACTCATGGCACAGGCACTGAATCGAGGAGCTTCAATGAGCCAATACAAGACGCCTCGATGTGTAAATTCTACGCACATCATTGAGCTTTTGGACTCGAACGTTGTTACCAAATACTTCAGTAAAGAATGCCCAAAAGTGGATACCAACGCTCTGCACCGTCTTCTTTCCAAGTAG

Protein sequence

MVSCSSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRVGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTSRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLSK

Homology

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BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 2.0e-141
Identity = 265/499 (53.11%), Postives = 328/499 (65.73%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P  EEE  RR   +  +MP M Q  P   +  GKGM+F F K E KT 
Sbjct: 109 SSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQFVPG--LENGKGMYFLFIKSESKTP 168

Query: 65  SGIVAHTATTSLCKRANL---------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K ++          N TSP + ILC+DS QS+Y Q+LCGLCQ+  V 
Sbjct: 169 GGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVL 228

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNILNPNAQLADFI 184
           RV                            GTL++ ITDPSVREAV  IL P+ +LADF+
Sbjct: 229 RVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFV 288

Query: 185 EAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
           E EC K +     +RLWPN KY++ IVTG+MSQY+P L+YYSN LP+V   Y +SEC+ G
Sbjct: 289 EFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG 348

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKL 304
           +NL PLC P   SYTLIP+MAYFEFLP+ R              + +  +ELVDLVDVKL
Sbjct: 349 VNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKL 408

Query: 305 GHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAE 364
           G EYELV+TTYAG    L RY +GD+LRVTGFKNKAP FSF+CRKNVVLS+D +KTDE E
Sbjct: 409 GQEYELVVTTYAG----LCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVE 468

Query: 365 LHDAVQEAARLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFA 424
           L +AV+ A   L PF A++++YTS+AD+S  PGHYVL+WE+ + G   IP S++EDCC A
Sbjct: 469 LQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLA 528

Query: 425 IEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHI 451
           +EES N  YR  R  +K I PLEIKIV+ GTF+KLM  A++ GAS++QYKTPRCV    I
Sbjct: 529 VEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI 588

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BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.2e-140
Identity = 263/499 (52.71%), Postives = 326/499 (65.33%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P  EEE  RR   +  +MP M Q  P   + KGKGM+F F K E KT 
Sbjct: 109 SSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQFVPG--LDKGKGMYFLFIKSESKTP 168

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K ++  N         TSP   ILC+DS QS+Y Q+LCGLCQ+  V 
Sbjct: 169 GGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVL 228

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNILNPNAQLADFI 184
           RV                            GTL+++ITD SVREAV  IL P+ +LADF+
Sbjct: 229 RVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEITDSSVREAVGEILKPDPKLADFV 288

Query: 185 EAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
           E+EC K +     +RLWPN KY++ IVTG+MSQY+P L+YYSN LP+V   Y +SEC+ G
Sbjct: 289 ESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG 348

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKL 304
           +NL PLC P   SYTLIP MAYFEFLP+ R              + +  +ELVDLVDVKL
Sbjct: 349 VNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKL 408

Query: 305 GHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAE 364
           G EYELV+TTYAG    LYRY +GD+L V GFKN AP FSF+CRKNVVLS+D +KTDE E
Sbjct: 409 GQEYELVVTTYAG----LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVE 468

Query: 365 LHDAVQEAARLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFA 424
           L +AV+ A   L PF A++++YTS+AD+S  PGHYVL+WE+ + G   IP S++EDCC  
Sbjct: 469 LQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLT 528

Query: 425 IEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHI 451
           IEESLN  YR  R  +K I PLEIK+V+ GTF+KLM  A++ GAS++QYKTPRCV    I
Sbjct: 529 IEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI 588

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BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 3.1e-126
Identity = 237/489 (48.47%), Postives = 313/489 (64.01%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P   +E  RR   +  +MP M Q    S + KGK M+  F K E +T 
Sbjct: 109 SSGTSGGERKLMPTIADEMNRRSLLYSLLMPVMSQSV--SGLDKGKAMYLLFVKAESRTP 168

Query: 65  SGIVAHTATTSLCKRANL---------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  +             + TSP + ILC DS QS+Y QLLCGL     V 
Sbjct: 169 GGLAARPVLTSYYRSRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVL 228

Query: 125 RVGT----------------------------LNTKITDPSVREAVMNILNPNAQLADFI 184
           RVG                             L+ +ITD  VR+AV  +L  +  LAD I
Sbjct: 229 RVGAVFASGFLRAIHFLEKHWARLCHDIRTGELDPEITDRVVRDAVGRVLRADPALADAI 288

Query: 185 EAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
           E EC++ +      RLWP  KYI+ IVTG+MSQY+P L +Y   LP+    Y +SEC+ G
Sbjct: 289 EDECARASWEGIIRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFG 348

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVS-RELVDLVDVKLGHEYELVITT 304
           LNL+P+C P   +YTLIPTM Y+EFLP++   ++ E S R+LVDLVDVKLGHEYELV+TT
Sbjct: 349 LNLNPMCKPSDVAYTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTT 408

Query: 305 YAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAAR 364
           Y+G    LYRY +GD+LRV GFKNKAP FSFV R+NV LS+D +KTDE ELH AV  A +
Sbjct: 409 YSG----LYRYRVGDVLRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQ 468

Query: 365 LLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYR 424
            L+PFGA++ +YTS+AD++  PGHYVL+WE+   G  ++P+S++E+CC ++EE+LN  YR
Sbjct: 469 HLAPFGASLVEYTSYADAATIPGHYVLFWELRA-GSTAVPASVFEECCLSVEEALNSVYR 528

Query: 425 YLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVT 451
             R+ ++ I PLEI++V  GTF+KLM  A++RGAS++QYK PRCV    ++ELLD+ V  
Sbjct: 529 QGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQG 588

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BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.5e-123
Identity = 239/495 (48.28%), Postives = 311/495 (62.83%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P  EEE  RR   +  +MP M +  P   + KGK M+  F K E +T 
Sbjct: 113 SSGTSGGERKLMPTIEEEMERRSGLYSLLMPVMSRQVPG--LDKGKAMYLYFVKSEWRTP 172

Query: 65  SGIVAHTATTSLCK-RANLNN--------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  + R  L          TSP + +LC D+ QS+Y QL+CGL     V 
Sbjct: 173 GGLPARPVLTSFYRSRYFLERPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVL 232

Query: 125 RVGT----------------------------LNTKITDPSVREAVMNIL-NPNAQLADF 184
           RVG                             L+  +TDP+VR AV  +L   +  LAD 
Sbjct: 233 RVGAVFASGFLRAIRFLEKHWPSLCRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADA 292

Query: 185 IEAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFL 244
           IEAEC++ +      R+WP+ KYI+ IVTG+M+QY+P L +Y   LP+    Y +SEC+ 
Sbjct: 293 IEAECARPSWQGIIRRVWPSTKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYF 352

Query: 245 GLNLDPLCSPDRTSYTLIPTMAYFEFLPIDR----TKSSREVSRELVDLVDVKLGHEYEL 304
           GLNL+P+C P   +YTLIPTM YFEFLP++       +     R LVDLVDVKLGHEYEL
Sbjct: 353 GLNLNPMCKPSEVAYTLIPTMCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYEL 412

Query: 305 VITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQ 364
           V+TTY+G    LYRY +GD+LRV GFKN AP F+FV RKNV LS+D +KTDEAELH AV 
Sbjct: 413 VVTTYSG----LYRYRVGDVLRVAGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVT 472

Query: 365 EAARLLSPFGAAIADYTSHAD-SSKFPGHYVLYWEIH-VKGGISIPSSIYEDCCFAIEES 424
           EA + L+PFGA++ +YTS+AD ++  PGHYVL+WE+    GG  +P+S++EDCC A+EE 
Sbjct: 473 EAVQHLAPFGASLVEYTSYADTATTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEG 532

Query: 425 LNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELL 451
           LN  YR  R+ ++ I PLEI++V  GTF+KLM  AL+RGAS++QYK PRCV    ++ELL
Sbjct: 533 LNSVYRQCRAADRSIGPLEIRVVADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELL 592

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BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 433.0 bits (1112), Expect = 4.4e-120
Identity = 230/489 (47.03%), Postives = 301/489 (61.55%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTS GE KL+P   +E  RR   +  +MP M    P   + KGKG++F F K E KT 
Sbjct: 114 SSGTSAGERKLMPTIMDELDRRQLLYSLLMPVMNLYVPG--LDKGKGLYFLFVKSETKTP 173

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K  +  N         TSP   ILC D+ QS+Y Q++CGLCQ + V 
Sbjct: 174 GGLTARPVLTSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVL 233

Query: 125 RVGT----------------------------LNTKITDPSVREAVMNILNPNAQLADFI 184
           R+G                             L  ++TDPSVREAV  IL P+ +LA  I
Sbjct: 234 RLGAVFASGLLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILLPDPELAKLI 293

Query: 185 EAECSKG-----TSRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
            AECSKG      +R+WPN KY++ IVTG+M+QY+P L +YS  LP+    Y +SEC+ G
Sbjct: 294 RAECSKGDWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG 353

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTY 304
           LNL P+C P   SYT++P M YFEFLP+D T ++   + +LVDL  V++G EYELVITTY
Sbjct: 354 LNLRPMCDPSEVSYTIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTY 413

Query: 305 AGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARL 364
           AG    L RY +GD+LRVTGF N AP F FV RKNV+LS++ +KTDEAEL  AV+ A+ L
Sbjct: 414 AG----LNRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASAL 473

Query: 365 LSPFGAAIADYTSHADSSKFPGHYVLYWEIHVK--GGISIPSSIYEDCCFAIEESLNGPY 424
           L P GA++ +YTS A + + PGHYV+YWE+  K  G   + +     CC  +EE+LN  Y
Sbjct: 474 LRPHGASVVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADTLGRCCLEMEEALNTVY 533

Query: 425 RYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVV 450
           R  R  +  I PLEI++V+ GTFE+LM  A++RGAS++QYK PRCV    I+ELLDS VV
Sbjct: 534 RQSRVADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVV 593

[Close]

BLAST of Cp4.1LG16g04550 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 447/491 (91.04%), Postives = 451/491 (91.85%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR
Sbjct: 101 SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 160

Query: 65  SGIVAHTATTSLCKRANLNNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRVG------ 124
           SGIVAHTATTSLCKRANLNNTSPKDIILCTD+NQSLYCQLLCGLCQNDIVFRVG      
Sbjct: 161 SGIVAHTATTSLCKRANLNNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFAST 220

Query: 125 ----------------------TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT- 184
                                 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT 
Sbjct: 221 LIHVLKFLEDHWVVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTW 280

Query: 185 ----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 244
               +RLWPNAKYINAIVTGSM+QYLPKLNYYSNHLPIVSDHYG SECFLGLNLDPLCSP
Sbjct: 281 EGIITRLWPNAKYINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSP 340

Query: 245 DRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGKSVWLYR 304
           DRTSYTLIPTMAYFEFLPIDRTKSSRE+SRELVDLVDVKLGHEYELVITTYAG    LYR
Sbjct: 341 DRTSYTLIPTMAYFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAG----LYR 400

Query: 305 YCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 364
           YCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA
Sbjct: 401 YCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 460

Query: 365 DYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 424
           DYTSHADSSK PGHYVLYWEIHVKGGISIPSSIYEDCCFAIEE LNGPYRYLRSHEKCIN
Sbjct: 461 DYTSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCIN 520

Query: 425 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKV 462
           PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKV
Sbjct: 521 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKV 580

[Close]

BLAST of Cp4.1LG16g04550 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 870 bits (2248), Expect = 1.31e-315
Identity = 442/490 (90.20%), Postives = 447/490 (91.22%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR
Sbjct: 101 SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 160

Query: 65  SGIVAHTATTSLCKRANLNNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRVG------ 124
           SGIVAHTATTSLCKRANLNNTSPKDIILCTD+NQSLYCQLLCGLCQNDIVFRVG      
Sbjct: 161 SGIVAHTATTSLCKRANLNNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFAST 220

Query: 125 ----------------------TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT- 184
                                 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT 
Sbjct: 221 LIHVLKFLEDHWVVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTW 280

Query: 185 ----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 244
               +RLWPNAKYINAIVTGSM+QYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP
Sbjct: 281 EGIITRLWPNAKYINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 340

Query: 245 DRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGKSVWLYR 304
           DRTSYTLIPTMAYFEFLPIDRTKSSRE+SRELVDLVDVKLGHEYELVITTYAG    LYR
Sbjct: 341 DRTSYTLIPTMAYFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAG----LYR 400

Query: 305 YCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 364
           Y MGDIL VTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA
Sbjct: 401 YRMGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 460

Query: 365 DYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 424
           DYTSHADSSK PGHYVLYWEIHVKGGISIPSSIYEDCCFAIEE LNGPYRYLRSHEKCIN
Sbjct: 461 DYTSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCIN 520

Query: 425 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKV 461
           PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVV KYFSK+CPKV
Sbjct: 521 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKV 580

[Close]

BLAST of Cp4.1LG16g04550 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 627 bits (1616), Expect = 2.25e-219
Identity = 334/499 (66.93%), Postives = 383/499 (76.75%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KLIPMYEEEF+RRLSFF+Y+MPRMKQLFPN    KGKGM+F FAK E KT+
Sbjct: 101 SSGTSGGETKLIPMYEEEFVRRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
           +GI+  +  TSL KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QND+VFR+
Sbjct: 161 AGIIVRSIFTSLHKRSSNLKSKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRL 220

Query: 125 GTL----------------------------NTKITDPSVREAVMNIL-NPNAQLADFIE 184
           G L                            N KIT+ SVRE+VM I+  PN ++AD IE
Sbjct: 221 GALFASTLIHVFKFLENHWVVLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            ECSKG+      RLWPN KYINAIVTGSMSQY+P LN+Y+N+LPIVSDHYG+SECFLGL
Sbjct: 281 HECSKGSWEGIIKRLWPNTKYINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYA 304
           NLDPLC P   SYTLIPTMAYFEFLPIDRT  + EV+++LVDLVDVKLG EYELVITT+A
Sbjct: 341 NLDPLCDPYEISYTLIPTMAYFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFA 400

Query: 305 GKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLL 364
           G    LYRY MGDI+RVTGF  KAPNFSFVCRKNVVLSL+ EKTDEA LH AV+E   +L
Sbjct: 401 G----LYRYRMGDIIRVTGFTKKAPNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVL 460

Query: 365 SPFGAAIADYTSHADSSKFPGHYVLYWEI----HVKGGIS--IPSSIYEDCCFAIEESLN 424
            PFGA I DYTS+ADSS  PGHYVLY+E+    H K  IS  IPSS+YEDCCFAIEE+LN
Sbjct: 461 KPFGATIVDYTSYADSSTIPGHYVLYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLN 520

Query: 425 GPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELL 454
             YR  RSHEKCI+PLEIKIVK GTFEKLM  A+NRGAS+++YKTPRC+NS   HII+LL
Sbjct: 521 SLYRLARSHEKCIDPLEIKIVKAGTFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLL 580

[Close]

BLAST of Cp4.1LG16g04550 vs. NCBI nr
Match: XP_008448232.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0062188.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa] >TYK04840.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 605 bits (1561), Expect = 3.52e-211
Identity = 320/496 (64.52%), Postives = 369/496 (74.40%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSG E KLIPMYE+EF+RRLSFF Y+ PRMKQLFPN    K KG++F FAK E KT+
Sbjct: 101 SSGTSGEEHKLIPMYEQEFVRRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
            GIV  +  T+L KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QND+VFRV
Sbjct: 161 GGIVVRSIFTNLHKRSSNLRSMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRV 220

Query: 125 G----------------------------TLNTKITDPSVREAVMNIL-NPNAQLADFIE 184
                                        T+N KIT  SVRE+VM I+  PN ++AD IE
Sbjct: 221 SALFASTLIHVFKFLENHWIDLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            EC KG      +RLWPNAKYINA+VTGSMSQY+P LNYY+N+LPIVSDHYG+SECFLGL
Sbjct: 281 NECGKGRWEGIITRLWPNAKYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYA 304
           NLDPLC+PD  SYTLIPTMAYFEFLPID      EV++ LVDLVDVKLGHEYELVITT+A
Sbjct: 341 NLDPLCNPDEVSYTLIPTMAYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFA 400

Query: 305 GKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLL 364
           G    LYRYCMGD++RVTGFKNKAPNFSFV RKNVVL++  EKTDEA LH AV+E  R++
Sbjct: 401 G----LYRYCMGDVVRVTGFKNKAPNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVM 460

Query: 365 SPFGAAIADYTSHADSSKFPGHYVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNG 424
             FGA I DYT++AD S  PGHYVLYWE+ +     +    IP S++EDCC AIE SLN 
Sbjct: 461 KQFGATIVDYTTYADISTIPGHYVLYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNL 520

Query: 425 PYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELLD 452
            YR  RSHEKCINPLEIKIVK GTFEKLM  ALNRGAS++QYKTPRC+NS+  HII+LL+
Sbjct: 521 LYRLARSHEKCINPLEIKIVKAGTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLE 580

[Close]

BLAST of Cp4.1LG16g04550 vs. NCBI nr
Match: XP_004140044.1 (indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN46710.1 hypothetical protein Csa_021088 [Cucumis sativus])

HSP 1 Score: 598 bits (1541), Expect = 4.53e-208
Identity = 318/499 (63.73%), Postives = 373/499 (74.75%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KLIPMYE+EF+RRLSFF Y+MPRMKQLFP+    K KG++F FAK E KT+
Sbjct: 101 SSGTSGGEHKLIPMYEQEFVRRLSFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
            GI+  +  T+L KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QN++VFRV
Sbjct: 161 GGIIVRSIFTNLHKRSSNLESMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRV 220

Query: 125 G----------------------------TLNTKITDPSVREAVMNIL-NPNAQLADFIE 184
                                        T+N KIT+ SVRE++M I+  PN ++AD IE
Sbjct: 221 SALFASTLIHVFKFLENHWVDLATDIRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            EC KG      +RLWPNAKYINAIVTGSMSQY+P LNYY+N+LPIVSDHYG+SECFLGL
Sbjct: 281 NECRKGRWEGIITRLWPNAKYINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSR-EVSRELVDLVDVKLGHEYELVITTY 304
           NLDPLC+ D  SYTLIPTMAYFEFLPID       EV+++LVDLVDVKLG EYELVITT+
Sbjct: 341 NLDPLCNHDEVSYTLIPTMAYFEFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTF 400

Query: 305 AGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARL 364
           AG    LYRYCMGDI+RVTGFKNKAP+F FV RKNVVL++  EKTDEA LH AV+E  R+
Sbjct: 401 AG----LYRYCMGDIVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRV 460

Query: 365 LSPFGAAIADYTSHADSSKFPGHYVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLN 424
           +  FGA I DYT++AD S  PGHYVLYWE+ +     +    IPSS++EDCC  IE SLN
Sbjct: 461 MKSFGAKIVDYTTYADLSTIPGHYVLYWELTMDDLKEQNSNDIPSSVFEDCCLDIENSLN 520

Query: 425 GPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELL 454
             YR  RSHEKCINPLEIKIVK GTFEKLM  AL+RGAS++QYKTPRC+NS+  HII+LL
Sbjct: 521 LLYRLARSHEKCINPLEIKIVKAGTFEKLMQLALDRGASITQYKTPRCLNSSQIHIIQLL 580

[Close]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 870 bits (2248), Expect = 6.35e-316
Identity = 442/490 (90.20%), Postives = 447/490 (91.22%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR
Sbjct: 101 SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 160

Query: 65  SGIVAHTATTSLCKRANLNNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRVG------ 124
           SGIVAHTATTSLCKRANLNNTSPKDIILCTD+NQSLYCQLLCGLCQNDIVFRVG      
Sbjct: 161 SGIVAHTATTSLCKRANLNNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFAST 220

Query: 125 ----------------------TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT- 184
                                 TLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGT 
Sbjct: 221 LIHVLKFLEDHWVVLASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTW 280

Query: 185 ----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 244
               +RLWPNAKYINAIVTGSM+QYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP
Sbjct: 281 EGIITRLWPNAKYINAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSP 340

Query: 245 DRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAGKSVWLYR 304
           DRTSYTLIPTMAYFEFLPIDRTKSSRE+SRELVDLVDVKLGHEYELVITTYAG    LYR
Sbjct: 341 DRTSYTLIPTMAYFEFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAG----LYR 400

Query: 305 YCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 364
           Y MGDIL VTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA
Sbjct: 401 YRMGDILGVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIA 460

Query: 365 DYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEESLNGPYRYLRSHEKCIN 424
           DYTSHADSSK PGHYVLYWEIHVKGGISIPSSIYEDCCFAIEE LNGPYRYLRSHEKCIN
Sbjct: 461 DYTSHADSSKIPGHYVLYWEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCIN 520

Query: 425 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKV 461
           PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVV KYFSK+CPKV
Sbjct: 521 PLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKV 580

[Close]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 1.71e-211
Identity = 320/496 (64.52%), Postives = 369/496 (74.40%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSG E KLIPMYE+EF+RRLSFF Y+ PRMKQLFPN    K KG++F FAK E KT+
Sbjct: 101 SSGTSGEEHKLIPMYEQEFVRRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
            GIV  +  T+L KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QND+VFRV
Sbjct: 161 GGIVVRSIFTNLHKRSSNLRSMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRV 220

Query: 125 G----------------------------TLNTKITDPSVREAVMNIL-NPNAQLADFIE 184
                                        T+N KIT  SVRE+VM I+  PN ++AD IE
Sbjct: 221 SALFASTLIHVFKFLENHWIDLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            EC KG      +RLWPNAKYINA+VTGSMSQY+P LNYY+N+LPIVSDHYG+SECFLGL
Sbjct: 281 NECGKGRWEGIITRLWPNAKYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYA 304
           NLDPLC+PD  SYTLIPTMAYFEFLPID      EV++ LVDLVDVKLGHEYELVITT+A
Sbjct: 341 NLDPLCNPDEVSYTLIPTMAYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFA 400

Query: 305 GKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLL 364
           G    LYRYCMGD++RVTGFKNKAPNFSFV RKNVVL++  EKTDEA LH AV+E  R++
Sbjct: 401 G----LYRYCMGDVVRVTGFKNKAPNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVM 460

Query: 365 SPFGAAIADYTSHADSSKFPGHYVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNG 424
             FGA I DYT++AD S  PGHYVLYWE+ +     +    IP S++EDCC AIE SLN 
Sbjct: 461 KQFGATIVDYTTYADISTIPGHYVLYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNL 520

Query: 425 PYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELLD 452
            YR  RSHEKCINPLEIKIVK GTFEKLM  ALNRGAS++QYKTPRC+NS+  HII+LL+
Sbjct: 521 LYRLARSHEKCINPLEIKIVKAGTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLE 580

[Close]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 1.71e-211
Identity = 320/496 (64.52%), Postives = 369/496 (74.40%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSG E KLIPMYE+EF+RRLSFF Y+ PRMKQLFPN    K KG++F FAK E KT+
Sbjct: 101 SSGTSGEEHKLIPMYEQEFVRRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
            GIV  +  T+L KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QND+VFRV
Sbjct: 161 GGIVVRSIFTNLHKRSSNLRSMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRV 220

Query: 125 G----------------------------TLNTKITDPSVREAVMNIL-NPNAQLADFIE 184
                                        T+N KIT  SVRE+VM I+  PN ++AD IE
Sbjct: 221 SALFASTLIHVFKFLENHWIDLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            EC KG      +RLWPNAKYINA+VTGSMSQY+P LNYY+N+LPIVSDHYG+SECFLGL
Sbjct: 281 NECGKGRWEGIITRLWPNAKYINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYA 304
           NLDPLC+PD  SYTLIPTMAYFEFLPID      EV++ LVDLVDVKLGHEYELVITT+A
Sbjct: 341 NLDPLCNPDEVSYTLIPTMAYFEFLPIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFA 400

Query: 305 GKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLL 364
           G    LYRYCMGD++RVTGFKNKAPNFSFV RKNVVL++  EKTDEA LH AV+E  R++
Sbjct: 401 G----LYRYCMGDVVRVTGFKNKAPNFSFVRRKNVVLNISNEKTDEAGLHKAVEEGGRVM 460

Query: 365 SPFGAAIADYTSHADSSKFPGHYVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLNG 424
             FGA I DYT++AD S  PGHYVLYWE+ +     +    IP S++EDCC AIE SLN 
Sbjct: 461 KQFGATIVDYTTYADISTIPGHYVLYWELTLDDLKEQNLNYIPCSVFEDCCLAIENSLNL 520

Query: 425 PYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELLD 452
            YR  RSHEKCINPLEIKIVK GTFEKLM  ALNRGAS++QYKTPRC+NS+  HII+LL+
Sbjct: 521 LYRLARSHEKCINPLEIKIVKAGTFEKLMQLALNRGASITQYKTPRCLNSSQIHIIQLLE 580

[Close]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL
Match: A0A0A0KAF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1)

HSP 1 Score: 598 bits (1541), Expect = 2.19e-208
Identity = 318/499 (63.73%), Postives = 373/499 (74.75%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KLIPMYE+EF+RRLSFF Y+MPRMKQLFP+    K KG++F FAK E KT+
Sbjct: 101 SSGTSGGEHKLIPMYEQEFVRRLSFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTK 160

Query: 65  SGIVAHTATTSLCKRA-NL------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
            GI+  +  T+L KR+ NL      NNTSP DIILCTDS QSLYCQLLCGL QN++VFRV
Sbjct: 161 GGIIVRSIFTNLHKRSSNLESMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRV 220

Query: 125 G----------------------------TLNTKITDPSVREAVMNIL-NPNAQLADFIE 184
                                        T+N KIT+ SVRE++M I+  PN ++AD IE
Sbjct: 221 SALFASTLIHVFKFLENHWVDLATDIRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIE 280

Query: 185 AECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGL 244
            EC KG      +RLWPNAKYINAIVTGSMSQY+P LNYY+N+LPIVSDHYG+SECFLGL
Sbjct: 281 NECRKGRWEGIITRLWPNAKYINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGL 340

Query: 245 NLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSR-EVSRELVDLVDVKLGHEYELVITTY 304
           NLDPLC+ D  SYTLIPTMAYFEFLPID       EV+++LVDLVDVKLG EYELVITT+
Sbjct: 341 NLDPLCNHDEVSYTLIPTMAYFEFLPIDMINDPNGEVNQQLVDLVDVKLGREYELVITTF 400

Query: 305 AGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARL 364
           AG    LYRYCMGDI+RVTGFKNKAP+F FV RKNVVL++  EKTDEA LH AV+E  R+
Sbjct: 401 AG----LYRYCMGDIVRVTGFKNKAPSFRFVRRKNVVLNISNEKTDEAGLHKAVEEGGRV 460

Query: 365 LSPFGAAIADYTSHADSSKFPGHYVLYWEIHV-----KGGISIPSSIYEDCCFAIEESLN 424
           +  FGA I DYT++AD S  PGHYVLYWE+ +     +    IPSS++EDCC  IE SLN
Sbjct: 461 MKSFGAKIVDYTTYADLSTIPGHYVLYWELTMDDLKEQNSNDIPSSVFEDCCLDIENSLN 520

Query: 425 GPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNST--HIIELL 454
             YR  RSHEKCINPLEIKIVK GTFEKLM  AL+RGAS++QYKTPRC+NS+  HII+LL
Sbjct: 521 LLYRLARSHEKCINPLEIKIVKAGTFEKLMQLALDRGASITQYKTPRCLNSSQIHIIQLL 580

[Close]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 588 bits (1515), Expect = 1.92e-204
Identity = 307/494 (62.15%), Postives = 358/494 (72.47%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           S+GTSGGE KLIP  E+EF R+LS  +Y+ PRMKQLFP   +  GK M F FAKVE  T+
Sbjct: 96  STGTSGGEQKLIPTIEQEFARKLSISNYMRPRMKQLFPG--LHNGKEMFFNFAKVESITK 155

Query: 65  SGIVAHTATTSLCKRANLNN-------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVFRV 124
           +GIVA + +TS  K  +LNN       TSP DI+ CTD  QSLYCQLLCGLCQN  V RV
Sbjct: 156 AGIVARSVSTSFLKSPHLNNVKVESSYTSPTDILCCTDVYQSLYCQLLCGLCQNQKVVRV 215

Query: 125 G----------------------------TLNTKITDPSVREAVMNILNPNAQLADFIEA 184
           G                            T+N KI D  VR++VMNIL P+ QLADFIE+
Sbjct: 216 GSLFASTLIYIFKFLENHWVLLANDIRTGTINAKIMDSYVRKSVMNILKPDPQLADFIES 275

Query: 185 ECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLGLN 244
           ECSKGT     +RLWPN K+INA+VTGSMS Y+P+LNYY   LP+++  Y +SECF G+N
Sbjct: 276 ECSKGTWEGIVTRLWPNTKFINAVVTGSMSHYIPQLNYYCKSLPLITVFYASSECFFGIN 335

Query: 245 LDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTYAG 304
           LDPLC P+  SYT IPT+AYFEFLP+DRT  S EV +E VDLVDVKLG EYE+V+TTYAG
Sbjct: 336 LDPLCKPEEISYTFIPTVAYFEFLPVDRTNDSGEVGQEFVDLVDVKLGQEYEIVVTTYAG 395

Query: 305 KSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLS 364
               LYRYC+GDI+RVTGFKNKAPNF FV RKNV+LSLD+EKTDEAELH+AV +   LLS
Sbjct: 396 ----LYRYCIGDIVRVTGFKNKAPNFRFVGRKNVILSLDVEKTDEAELHNAVNKVGSLLS 455

Query: 365 PFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISI-PSSIYEDCCFAIEESLNGPYRYL 424
            FGA + DYTS+ADSS FPGHYVLYWEI  K  ISI PSSIYEDCC AIEESL+  YR  
Sbjct: 456 SFGAILVDYTSYADSSTFPGHYVLYWEIQFKEEISIIPSSIYEDCCLAIEESLSSVYRRA 515

Query: 425 RSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKY 457
           RSH+K INPLEIKIV+  TF+KLM + +++GAS+SQYKTPRCV ST IIE LDS VV  Y
Sbjct: 516 RSHQKVINPLEIKIVEARTFDKLMERMVDQGASISQYKTPRCVKSTQIIEFLDSKVVANY 575

[Close]

BLAST of Cp4.1LG16g04550 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 503.8 bits (1296), Expect = 1.4e-142
Identity = 265/499 (53.11%), Postives = 328/499 (65.73%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P  EEE  RR   +  +MP M Q  P   +  GKGM+F F K E KT 
Sbjct: 109 SSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMSQFVPG--LENGKGMYFLFIKSESKTP 168

Query: 65  SGIVAHTATTSLCKRANL---------NNTSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K ++          N TSP + ILC+DS QS+Y Q+LCGLCQ+  V 
Sbjct: 169 GGLPARPVLTSYYKSSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVL 228

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNILNPNAQLADFI 184
           RV                            GTL++ ITDPSVREAV  IL P+ +LADF+
Sbjct: 229 RVGAVFASGFIRAIKFLEKHWIELVRDIRTGTLSSLITDPSVREAVAKILKPSPKLADFV 288

Query: 185 EAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
           E EC K +     +RLWPN KY++ IVTG+MSQY+P L+YYSN LP+V   Y +SEC+ G
Sbjct: 289 EFECKKSSWQGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG 348

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKL 304
           +NL PLC P   SYTLIP+MAYFEFLP+ R              + +  +ELVDLVDVKL
Sbjct: 349 VNLRPLCKPSEVSYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKL 408

Query: 305 GHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAE 364
           G EYELV+TTYAG    L RY +GD+LRVTGFKNKAP FSF+CRKNVVLS+D +KTDE E
Sbjct: 409 GQEYELVVTTYAG----LCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVE 468

Query: 365 LHDAVQEAARLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFA 424
           L +AV+ A   L PF A++++YTS+AD+S  PGHYVL+WE+ + G   IP S++EDCC A
Sbjct: 469 LQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLA 528

Query: 425 IEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHI 451
           +EES N  YR  R  +K I PLEIKIV+ GTF+KLM  A++ GAS++QYKTPRCV    I
Sbjct: 529 VEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPI 588

[Close]

BLAST of Cp4.1LG16g04550 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 500.4 bits (1287), Expect = 1.6e-141
Identity = 263/499 (52.71%), Postives = 326/499 (65.33%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTSGGE KL+P  EEE  RR   +  +MP M Q  P   + KGKGM+F F K E KT 
Sbjct: 109 SSGTSGGERKLMPTIEEELDRRSLLYSLLMPVMDQFVPG--LDKGKGMYFLFIKSESKTP 168

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K ++  N         TSP   ILC+DS QS+Y Q+LCGLCQ+  V 
Sbjct: 169 GGLPARPVLTSYYKSSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVL 228

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNILNPNAQLADFI 184
           RV                            GTL+++ITD SVREAV  IL P+ +LADF+
Sbjct: 229 RVGAVFASGFIRAIKFLEKHWPELARDIRTGTLSSEITDSSVREAVGEILKPDPKLADFV 288

Query: 185 EAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
           E+EC K +     +RLWPN KY++ IVTG+MSQY+P L+YYSN LP+V   Y +SEC+ G
Sbjct: 289 ESECRKTSWQGIITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFG 348

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKS-----------SREVSRELVDLVDVKL 304
           +NL PLC P   SYTLIP MAYFEFLP+ R              + +  +ELVDLVDVKL
Sbjct: 349 VNLRPLCKPSEVSYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKL 408

Query: 305 GHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAE 364
           G EYELV+TTYAG    LYRY +GD+L V GFKN AP FSF+CRKNVVLS+D +KTDE E
Sbjct: 409 GQEYELVVTTYAG----LYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVE 468

Query: 365 LHDAVQEAARLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPSSIYEDCCFA 424
           L +AV+ A   L PF A++++YTS+AD+S  PGHYVL+WE+ + G   IP S++EDCC  
Sbjct: 469 LQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLT 528

Query: 425 IEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHI 451
           IEESLN  YR  R  +K I PLEIK+V+ GTF+KLM  A++ GAS++QYKTPRCV    I
Sbjct: 529 IEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPI 588

[Close]

BLAST of Cp4.1LG16g04550 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 426.0 bits (1094), Expect = 3.8e-119
Identity = 232/489 (47.44%), Postives = 301/489 (61.55%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTS GE KL+P  +EE  RR   +  +MP M    P   + KGKGM+F F K E KT 
Sbjct: 107 SSGTSAGERKLMPTIKEELDRRQLLYSLLMPVMNLYVPG--LDKGKGMYFLFVKSETKTP 166

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K  +  +         TSP + ILC DS QS+Y Q+LCGL     V 
Sbjct: 167 GGLPARPVLTSYYKSEHFRSRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVL 226

Query: 125 RVGT----------------------------LNTKITDPSVREAVMNILNPNAQLADFI 184
           RVG                             L+++ITDPS+R+ +  IL P+  LA+FI
Sbjct: 227 RVGAVFASGLLRAIRFLQLHWSRFAHDIELGCLDSEITDPSIRQCMSGILKPDPVLAEFI 286

Query: 185 EAEC-----SKGTSRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFLG 244
             EC      K  +R+WPN KY++ IVTG+M+QY+P L YYS  LP+    Y +SEC+ G
Sbjct: 287 RRECKSDNWEKIITRIWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFG 346

Query: 245 LNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVITTY 304
           LNL+P+  P   SYT++P MAYFEF+P+  TK+        V+LVDV +G EYELV+TTY
Sbjct: 347 LNLNPMSKPSEVSYTIMPNMAYFEFIPLGGTKA--------VELVDVNIGKEYELVVTTY 406

Query: 305 AGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAARL 364
           AG    L RY +GDILRVTGF N AP F FV RKNV+LS+D +KTDE+EL  AV+ A+ +
Sbjct: 407 AG----LCRYRVGDILRVTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSI 466

Query: 365 L-SPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPS-SIYEDCCFAIEESLNGPY 424
           L    G+ +A+YTS+AD+S  PGHYVLYWE+ V+ G   PS      CC  +EESLN  Y
Sbjct: 467 LHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRDGARQPSHETLTRCCLGMEESLNSVY 526

Query: 425 RYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNVV 450
           R  R  +  + PLEI++V+ GTFE+LM  A++RGAS++QYK PRCVN T I+ELLDS VV
Sbjct: 527 RQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVV 581

[Close]

BLAST of Cp4.1LG16g04550 vs. TAIR 10
Match: AT1G28130.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.0e-115
Identity = 226/509 (44.40%), Postives = 309/509 (60.71%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTS G+PKL+P   EE  R+  F+  ++P M +      + +GKGM+  F K E+KT 
Sbjct: 99  SSGTSAGKPKLMPSTAEELERKTFFYSMLVPIMNKYVDG--LDEGKGMYLLFIKPEIKTP 158

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
           SG++A    TS  K  +  N         TSP   ILC DS QS+YCQLLCGL Q   V 
Sbjct: 159 SGLMARPVLTSYYKSQHFRNRPFNKYNVYTSPDQTILCQDSKQSMYCQLLCGLVQRSHVL 218

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNILN-PNAQLADF 184
           RV                            GT+ + ITD S R++V++ILN PN +LAD 
Sbjct: 219 RVGAVFASAFLRAVKFLEDHYKELCADIRTGTVTSWITDSSCRDSVLSILNGPNQELADE 278

Query: 185 IEAECSKGT-----SRLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECFL 244
           IE+EC++ +      R+WP AKY+  IVTGSM+QY+P L +YS  LP+VS  Y +SEC+ 
Sbjct: 279 IESECAEKSWEGILRRIWPKAKYVEVIVTGSMAQYIPTLEFYSGGLPLVSTMYASSECYF 338

Query: 245 GLNLDPLCSPDRTSYTLIPTMAYFEFLPID-------------RTKSSREVSRE--LVDL 304
           G+NL+PLC P   SYTL+P MAYFEFLP+D              T    +  +E  +V+L
Sbjct: 339 GINLNPLCDPADVSYTLLPNMAYFEFLPVDDKSHEEIHFATHSNTDDDDDALKEDLIVNL 398

Query: 305 VDVKLGHEYELVITTYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEK 364
           V+V++G  YE+VITT+ G    LYRY +GDIL+VTGF NKAP F FV R+NVVLS+D +K
Sbjct: 399 VNVEVGQYYEIVITTFTG----LYRYRVGDILKVTGFHNKAPQFRFVQRRNVVLSIDTDK 458

Query: 365 TDEAELHDAVQEAA--RLLSPFGAAIADYTSHADSSKFPGHYVLYWEI---HVKGGISIP 424
           T E +L +AV +A    L  P    + +YTS+AD+S  PGHYVL+WE+   H      + 
Sbjct: 459 TSEEDLLNAVTQAKLNHLQHPSSLLLTEYTSYADTSSIPGHYVLFWELKPRHSNDPPKLD 518

Query: 425 SSIYEDCCFAIEESLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYK 451
               EDCC  +E+ L+  YR  R+ +K I PLEI++V +GTF+ LM   +++G+S++QYK
Sbjct: 519 DKTMEDCCSEVEDCLDYVYRRCRNRDKSIGPLEIRVVSLGTFDSLMDFCVSQGSSLNQYK 578

[Close]

BLAST of Cp4.1LG16g04550 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.6e-115
Identity = 223/490 (45.51%), Postives = 300/490 (61.22%), Query Frame = 0


Query: 5   SSGTSGGEPKLIPMYEEEFMRRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTR 64
           SSGTS GE KL+P  +E+  RR   +  +MP M    P   + KGK ++F F K E KT 
Sbjct: 107 SSGTSAGERKLMPTIDEDMDRRQLLYSLLMPVMNLYVPG--LDKGKALYFLFVKTESKTP 166

Query: 65  SGIVAHTATTSLCKRANLNN---------TSPKDIILCTDSNQSLYCQLLCGLCQNDIVF 124
            G+ A    TS  K               TSP + ILC DS+QS+Y Q+LCGL     V 
Sbjct: 167 GGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVL 226

Query: 125 RV----------------------------GTLNTKITDPSVREAVMNIL-NPNAQLADF 184
           R+                            GTL+++I+DP+++E++  IL  P+ +LADF
Sbjct: 227 RLGAVFASGLLRAIGFLQTNWKELADDISTGTLSSRISDPAIKESMSKILTKPDQELADF 286

Query: 185 IEAECSKGTS------RLWPNAKYINAIVTGSMSQYLPKLNYYSNHLPIVSDHYGASECF 244
           I + C +  S      ++WPN KY++ IVTG+M+QY+P L YYS  LP+    Y +SE +
Sbjct: 287 ITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESY 346

Query: 245 LGLNLDPLCSPDRTSYTLIPTMAYFEFLPIDRTKSSREVSRELVDLVDVKLGHEYELVIT 304
            G+NL P+C P   SYT++P MAYFEFLP     + +    ELV+L DV++G EYELVIT
Sbjct: 347 FGINLKPMCKPSEVSYTIMPNMAYFEFLPHHEVPTEKS---ELVELADVEVGKEYELVIT 406

Query: 305 TYAGKSVWLYRYCMGDILRVTGFKNKAPNFSFVCRKNVVLSLDIEKTDEAELHDAVQEAA 364
           TYAG    L RY +GDIL+VTGF N AP F FV RKNV+LS++ +KTDEAEL  AV+ A+
Sbjct: 407 TYAG----LNRYRVGDILQVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENAS 466

Query: 365 RLLSPFGAAIADYTSHADSSKFPGHYVLYWEIHVKGGISIPS-SIYEDCCFAIEESLNGP 424
            LL   G  + +YTS+A++   PGHYV+YWE+ VK   + P+  +   CC  +EESLN  
Sbjct: 467 LLLGEQGTRVIEYTSYAETKTIPGHYVIYWELLVKDQTNPPNDEVMARCCLEMEESLNSV 526

Query: 425 YRYLRSHEKCINPLEIKIVKVGTFEKLMAQALNRGASMSQYKTPRCVNSTHIIELLDSNV 450
           YR  R  +K I PLEI++VK GTFE+LM  A++RGAS++QYK PRCV+ T I+ELLDS V
Sbjct: 527 YRQSRVADKSIGPLEIRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRV 586

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g04550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	2.0e-141	53.11	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	2.2e-140	52.71	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	3.1e-126	48.47	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	1.5e-123	48.28	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
A3BLS0	4.4e-120	47.03	Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399...	[more]

BLAST of Cp4.1LG16g04550 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570863.1	0.0	91.04	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	1.31e-315	90.20	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_038902067.1	2.25e-219	66.93	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]
XP_008448232.1	3.52e-211	64.52	PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0...	[more]
XP_004140044.1	4.53e-208	63.73	indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN46710.1 hypoth...	[more]

BLAST of Cp4.1LG16g04550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUU5	6.35e-316	90.20	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A5A7V3L5	1.71e-211	64.52	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BJT8	1.71e-211	64.52	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0KAF9	2.19e-208	63.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1	[more]
A0A6J1CIS9	1.92e-204	62.15	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]

BLAST of Cp4.1LG16g04550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	1.4e-142	53.11	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.6e-141	52.71	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	3.8e-119	47.44	Auxin-responsive GH3 family protein	[more]
AT1G28130.1	2.0e-115	44.40	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	2.6e-115	45.51	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 117..429 e-value: 4.9E-110 score: 368.9
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 116..451 coord: 5..119
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 5..119
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 116..451

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG16g04550.1	Cp4.1LG16g04550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity