Cp4.1LG16g03400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g03400 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.0e-23
Identity = 131/577 (22.70%), Postives = 225/577 (38.99%), Query Frame = 0

Query: 213 PPPPPPSPPRISAWDFLNFFETPAVGNYYGSYTPGRDPREVREEEGIPELEDVRYYQPEV 272
           PPPPPP PP  S WDF + F  P          P     E  EEE               
Sbjct: 108 PPPPPPPPPPSSTWDFWDPFIPP----------PPSSSEEEWEEE--------------- 167

Query: 273 VKKVHGDHNFAVDSGGKYSKAAVDDPLKVVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNK 332
                                      +      + AA  T P+ A   A     G    
Sbjct: 168 ----------------------TTTATRTATGTGSDAAVTTAPTTATPQASSVVSGFSKD 227

Query: 333 KLEGHGNGAPSSSAALKRGGGSRDVYEVVKEIEALFKKASEFGDEIAKMLE--------- 392
            +     G+  +    + G   +D+ E++KE++  F KA++ G  ++ +LE         
Sbjct: 228 TMTTTTTGSELAVVVSRNG---KDLMEIIKEVDEYFLKAADSGAPLSSLLEISTSITDFS 287

Query: 393 ----TGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSATLKKLY 452
               +G++     +     P +   R    +          V       G+ S+T+ +LY
Sbjct: 288 GHSKSGKMYSSSNYECNLNPTSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSHSSTVDRLY 347

Query: 453 MWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE------------------A 512
            WEKKLY EVK  E ++M HE+K  Q++RL  + AE  K E                  A
Sbjct: 348 AWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQLSVSSQA 407

Query: 513 IQTSINTLTR-------------------MWKGMLECHHAQFQAIKESRGLGHIRSGGKP 572
           IQ++ N + +                   MW+ M E H  Q   +++ + L  I S    
Sbjct: 408 IQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLNTIPSTEPT 467

Query: 573 SDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSP 632
           S+L  + TLQL+ E+  W  SF   + AQ+++ +SL  W L+  L++  + P     +  
Sbjct: 468 SELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGW-LRLSLFQFSKNPLVRSSYE- 527

Query: 633 GRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSV-----LQIWEHDKQEVRKTMIT 692
                  I+  C +W   +DR  +K   + +  F  +V      Q  EH +++  ++M+ 
Sbjct: 528 -----SKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKKRTESML- 587

Query: 693 NKDLERKAKRIDRDDQKL--------QKKIQALDK--KLIMVTGHVQGDGT--------- 711
            KD E+K+  +   + K         +KK   ++K  K+ M+ G  + + +         
Sbjct: 588 -KDFEKKSASLRALESKYSPYSVPESRKKNPVIEKRVKVEMLKGKAEEEKSKHEKSVSVT 625

BLAST of Cp4.1LG16g03400 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 1.2e-20
Identity = 171/743 (23.01%), Postives = 282/743 (37.95%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCA SKLD+  AV  C+DR   + EA+  RH  A AH  Y  SL+  G +L +F   G 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFAS-GE 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
             S    +P + L       S+  P+     R S S + S ++ PS S   +SS   S  
Sbjct: 61  PLSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVM 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           S+               NR           +        +  ++ +  P S+ R      
Sbjct: 121 ST-------------SSNR-----------RRKQQPKPRLPHILSESSPSSSPR---SER 180

Query: 181 SASSSGYYP--YPNNGYSNYGGGYGGYYGSSSSKPPPPPPS--------PPRISAWDFLN 240
           S      YP  Y N+ YS         +   +  PP PP S          + ++ +  N
Sbjct: 181 SNFMPNLYPSAYQNSTYSATPSHASSVWNWENFYPPSPPDSEFFNRKAQEKKHNSDNRFN 240

Query: 241 FFETPAVGNYYGSYTPGRDP-------REVREEEGIPELEDVRYYQPEVVKKVHGDHNFA 300
             +T  V + Y  +   +         R   EEE   E E+V+  + E       DH   
Sbjct: 241 DEDTETVRSEYDFFDTRKQKQKQFESMRNQVEEETETEREEVQCSEWE-----DHDHYST 300

Query: 301 VDSGGKYSKAAVDDPLKVVNK--------NVAAAAYQTKPSAAVEDAVQYEMGVVNKKLE 360
             S     +   DD  + +++        +   +    +         Q   G    K +
Sbjct: 301 TSSSDAAEEEEEDDDRESISEVGTRSEFGSTVRSNSMRRHHQQPSPMPQVYGGAEQSKYD 360

Query: 361 GHGNGAPSSSAALKRGGGS----------RDVYEVVKEIEALFKKASEFGDEIAKMLETG 420
              +   SS +   RGGG           RD+ E++  I+  F KA+  G+++++MLE G
Sbjct: 361 KADDATISSGS--YRGGGDIADMKMVVRHRDLKEIIDAIKENFDKAAASGEQVSQMLELG 420

Query: 421 QLPHQ-----------HKHAILTRPPASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSAT 480
           +               H  ++L+   ++     P A+           D    S +L +T
Sbjct: 421 RAELDRSFSQLKKTVIHSSSLLSNLSSTWTSKPPLAVKYRIDTTAL--DQPNSSKSLCST 480

Query: 481 LKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVEAIQTSINTLTR--- 540
           L +L  WEKKLY E+K  E  ++ HE+K  QL+    +G +  K++  + SI  L     
Sbjct: 481 LDRLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSLII 540

Query: 541 ----------------------------------MWKGMLECHHAQFQAIKESRGL-GHI 600
                                             MWK M + H  Q   +++ RGL    
Sbjct: 541 VTSQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRS 600

Query: 601 RSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDG 655
             G   S+L  + T  L+  + SW +SFS  I  Q++F  S++ W    LL   +E    
Sbjct: 601 GKGESTSELHRQATRDLESAVSSWHSSFSSLIKFQRDFIHSVHAWFKLTLLPVCQE---- 660

BLAST of Cp4.1LG16g03400 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 1.5e-13
Identity = 151/715 (21.12%), Postives = 268/715 (37.48%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIE--P 60
           MGC  SK++    V  C++R   + EA+  R   A AH  Y+ SL+    +L  F +  P
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  GYVYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHS 120
               S  ++   L         +   P  S       S +SS L  P+          H 
Sbjct: 61  SLAVSHHTAPVLLTTAAPALAPTPTPPPPS-------STASSSLPPPT-----PLLPKHQ 120

Query: 121 DHSSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQM 180
               P   T       P   R     GG   +++ ++ +++                  +
Sbjct: 121 QAPPPPPPTQSHQPPPPVAVRA--PRGGPRRLKVPHILSDS-----------------SV 180

Query: 181 GESASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPS------PPRISAWDFLNFF 240
              A SS   P      S+    +  +Y      PP PP S         +   + L   
Sbjct: 181 ASPARSSFRKPVVGTPSSSSAWDWENFY------PPSPPDSEFFDRRKADLEEANRLREL 240

Query: 241 ETPAVGNYYGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSK 300
           E       Y        P  ++EE+ + + +D R  +         D ++A  +  +   
Sbjct: 241 EEEEKARGY------LHPHHLKEEDEVDDDDDEREEEMHCGGWEDDDDHYASTTTSETRS 300

Query: 301 AAVDDPLKVVNKNVAAAAY-QTKPSAAVEDAVQYEMGVVNKKLE-GHGNGAPSSSAALKR 360
              +   +      A + Y  T PS      +   +   +++ E G  +   +++A ++ 
Sbjct: 301 EEGEMGNRSECGFAARSEYGGTAPSEYAAAPLPLPLRRRDERSEAGDSSSTVTAAAEMRM 360

Query: 361 GGGSRDVYEVVKEIEALFKKASEFGDEIAKMLET--GQLPHQ---------HKHAILTRP 420
               R + E+V  IE  F KA+E G+ ++++LE    QL            H +++L+  
Sbjct: 361 VIRHRTLAEIVAAIEEYFVKAAEAGNGVSELLEASRAQLDRNFRQLKKTVYHSNSLLS-S 420

Query: 421 PASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSH 480
            +S   +KP        +   +E   M   +  +TL++L  WEKKLY EVK  E +++ H
Sbjct: 421 LSSTWTSKPPLAVRYKLDTNALEMESMEGKSHGSTLERLLAWEKKLYQEVKARESVKIEH 480

Query: 481 ERKRHQLKRLHERGAEAQKVEAIQTSIN-------------------------------- 540
           E+K   L+ L  RG ++ K++  + SIN                                
Sbjct: 481 EKKLSTLQSLEYRGRDSTKLDKTKASINKLQSLIIVTSQAATTTSSAIVRVRDNELAPQL 540

Query: 541 -----TLTRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTT 600
                 L  MW+ M   H  Q + +++ RGL         SDL    T  L+  + +W +
Sbjct: 541 VELCFALLSMWRSMNHFHEIQNEIVQQVRGLVDNSMAESTSDLHRLATRDLEAAVSAWHS 600

Query: 601 SFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLD 655
           +F+  I  Q+++ R+L  WL   L       P         R     +   C++W Q LD
Sbjct: 601 NFNRLIKYQRDYIRALYGWLKLTLFQVDSNIPQEAYTSLISR----ELTTFCDEWKQALD 660

BLAST of Cp4.1LG16g03400 vs. NCBI nr
Match: XP_023512673.1 (nitrate regulatory gene2 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1400 bits (3624), Expect = 0.0
Identity = 719/756 (95.11%), Postives = 719/756 (95.11%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVF 420
           VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVF
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVF 420

Query: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE 480
           VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE
Sbjct: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE 480

Query: 481 AIQTSINTL-------------------------------------TRMWKGMLECHHAQ 540
           AIQTSINTL                                     TRMWKGMLECHHAQ
Sbjct: 481 AIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHHAQ 540

Query: 541 FQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLL 600
           FQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLL
Sbjct: 541 FQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLL 600

Query: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660
           KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI
Sbjct: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660

Query: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQA 719
           WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQA
Sbjct: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQA 720

BLAST of Cp4.1LG16g03400 vs. NCBI nr
Match: XP_022943509.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1380 bits (3571), Expect = 0.0
Identity = 711/757 (93.92%), Postives = 713/757 (94.19%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPD NRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDENRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFA DSGGKYSKAA+DDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAEDSGGKYSKAAMDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSS ALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSTALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAA-EVV 420
           VKEIEALFKKASEFGDEIAKMLE GQLPHQHKHAILTRPPASRRRAKPSAM GAAA EVV
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLEMGQLPHQHKHAILTRPPASRRRAKPSAMTGAAAAEVV 420

Query: 421 FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV 480
           FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV
Sbjct: 421 FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV 480

Query: 481 EAIQTSINTL-------------------------------------TRMWKGMLECHHA 540
           EAIQTSINTL                                     TRMWKGMLECHHA
Sbjct: 481 EAIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHHA 540

Query: 541 QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL 600
           QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL
Sbjct: 541 QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL 600

Query: 601 LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ 660
           LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ
Sbjct: 601 LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ 660

Query: 661 IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQ 719
           IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDG+ SLQ
Sbjct: 661 IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGSSSLQ 720

BLAST of Cp4.1LG16g03400 vs. NCBI nr
Match: KAG6570985.1 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1376 bits (3561), Expect = 0.0
Identity = 709/757 (93.66%), Postives = 711/757 (93.92%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFA DSGGKYSKAA+DDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAEDSGGKYSKAAMDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSS ALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSTALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAA--EV 420
           VKEIEALFKKASEFGDEIAKMLE GQLPHQHKHAILTRPPASRRRAKPSAMA AAA  EV
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLEMGQLPHQHKHAILTRPPASRRRAKPSAMAAAAAAEEV 420

Query: 421 VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQK 480
           VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSH RKRHQLKRLHERGAEAQK
Sbjct: 421 VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHVRKRHQLKRLHERGAEAQK 480

Query: 481 VEAIQTSINTL-------------------------------------TRMWKGMLECHH 540
           VEAIQTSINTL                                     TRMWKGMLECHH
Sbjct: 481 VEAIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHH 540

Query: 541 AQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNW 600
           AQFQ IKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNW
Sbjct: 541 AQFQVIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNW 600

Query: 601 LLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVL 660
           LLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVL
Sbjct: 601 LLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVL 660

Query: 661 QIWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISL 718
           QIWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDG+ SL
Sbjct: 661 QIWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGSSSL 720

BLAST of Cp4.1LG16g03400 vs. NCBI nr
Match: XP_022985552.1 (nitrate regulatory gene2 protein-like [Cucurbita maxima])

HSP 1 Score: 1358 bits (3516), Expect = 0.0
Identity = 698/756 (92.33%), Postives = 703/756 (92.99%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAV LCRDRCAFLDEAIRFRHAFA+AHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVVLCRDRCAFLDEAIRFRHAFAEAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPDGN GGFVS GGGFVQMNYMKNNAMQSVVHQQMPI+TERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDGNCGGFVSSGGGFVQMNYMKNNAMQSVVHQQMPINTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQP VVKKVHGDHNFA DSGGKYSKAAVDDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPVVVKKVHGDHNFAEDSGGKYSKAAVDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSA VED+VQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSATVEDSVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVF 420
           VKEIEALFKKASEFGDEIAKMLE GQLPHQ KHA L RPPASR+RAKPSAMAGAAAEVVF
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLEMGQLPHQRKHAFLARPPASRQRAKPSAMAGAAAEVVF 420

Query: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE 480
           VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKR QLKRLHERGAEA+KVE
Sbjct: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRRQLKRLHERGAEAKKVE 480

Query: 481 AIQTSINTL-------------------------------------TRMWKGMLECHHAQ 540
           AIQTSINTL                                     TRMWKGMLECHHAQ
Sbjct: 481 AIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHHAQ 540

Query: 541 FQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLL 600
           FQAIKES GLGHIRSGGKPSD DLRVTLQLDHELISWTTSFSGWISAQKNF RSLNNWLL
Sbjct: 541 FQAIKESHGLGHIRSGGKPSDFDLRVTLQLDHELISWTTSFSGWISAQKNFMRSLNNWLL 600

Query: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660
           KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI
Sbjct: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660

Query: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQA 719
           WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGT SLQA
Sbjct: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTSSLQA 720

BLAST of Cp4.1LG16g03400 vs. NCBI nr
Match: KAG7010823.1 (hypothetical protein SDJN02_27619, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1337 bits (3460), Expect = 0.0
Identity = 689/722 (95.43%), Postives = 700/722 (96.95%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFA DSGGKYSKAA+DDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAEDSGGKYSKAAMDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSR-DVYE 360
           VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSR DVYE
Sbjct: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRGDVYE 360

Query: 361 VVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAA-EV 420
           VVKEIEALFKKASEFGDEIAKMLE GQLPHQHKHAILTRPPASRRRAKPSAMA AAA EV
Sbjct: 361 VVKEIEALFKKASEFGDEIAKMLEMGQLPHQHKHAILTRPPASRRRAKPSAMASAAAAEV 420

Query: 421 VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQK 480
           VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQK
Sbjct: 421 VFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQK 480

Query: 481 VEAIQTSINTLTRMWKGMLECH-----HAQFQA--IKESRGLGHIRSGGKPSDLDLRVTL 540
           VEAIQTSINTL+   K  ++ +     +A+  +  + +SRGLGHIRSGGKPSDLDLRVTL
Sbjct: 481 VEAIQTSINTLSTNLKIAIQVNQDVERYARMPSCSVPKSRGLGHIRSGGKPSDLDLRVTL 540

Query: 541 QLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIF 600
           QLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIF
Sbjct: 541 QLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIF 600

Query: 601 VICNQWSQVLDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRD 660
           VICNQWSQVLDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRD
Sbjct: 601 VICNQWSQVLDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRD 660

Query: 661 DQKLQKKIQALDKKLIMVTGHVQGDGTISLQAGLQSIFEALERFATDSMKAYEELLQRSA 713
           DQKLQKKIQALDKKLIMVTGHVQGDG+ SLQAGLQSIFEALERFATDSMKAYEELLQRSA
Sbjct: 661 DQKLQKKIQALDKKLIMVTGHVQGDGSSSLQAGLQSIFEALERFATDSMKAYEELLQRSA 720

BLAST of Cp4.1LG16g03400 vs. ExPASy TrEMBL
Match: A0A6J1FUJ3 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448260 PE=4 SV=1)

HSP 1 Score: 1380 bits (3571), Expect = 0.0
Identity = 711/757 (93.92%), Postives = 713/757 (94.19%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPD NRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDENRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFA DSGGKYSKAA+DDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAEDSGGKYSKAAMDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSS ALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSTALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAA-EVV 420
           VKEIEALFKKASEFGDEIAKMLE GQLPHQHKHAILTRPPASRRRAKPSAM GAAA EVV
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLEMGQLPHQHKHAILTRPPASRRRAKPSAMTGAAAAEVV 420

Query: 421 FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV 480
           FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV
Sbjct: 421 FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKV 480

Query: 481 EAIQTSINTL-------------------------------------TRMWKGMLECHHA 540
           EAIQTSINTL                                     TRMWKGMLECHHA
Sbjct: 481 EAIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHHA 540

Query: 541 QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL 600
           QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL
Sbjct: 541 QFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWL 600

Query: 601 LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ 660
           LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ
Sbjct: 601 LKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQ 660

Query: 661 IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQ 719
           IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDG+ SLQ
Sbjct: 661 IWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGSSSLQ 720

BLAST of Cp4.1LG16g03400 vs. ExPASy TrEMBL
Match: A0A6J1J562 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483590 PE=4 SV=1)

HSP 1 Score: 1358 bits (3516), Expect = 0.0
Identity = 698/756 (92.33%), Postives = 703/756 (92.99%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDLPAV LCRDRCAFLDEAIRFRHAFA+AHTAYILSLQAVGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDDLPAVVLCRDRCAFLDEAIRFRHAFAEAHTAYILSLQAVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH
Sbjct: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLHQTHGDFLDYPDGN GGFVS GGGFVQMNYMKNNAMQSVVHQQMPI+TERVYQMGE
Sbjct: 121 SSPLHQTHGDFLDYPDGNCGGFVSSGGGFVQMNYMKNNAMQSVVHQQMPINTERVYQMGE 180

Query: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240
           SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY
Sbjct: 181 SASSSGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGNY 240

Query: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLK 300
           YGSYTPGRDPREVREEEGIPELEDVRYYQP VVKKVHGDHNFA DSGGKYSKAAVDDPLK
Sbjct: 241 YGSYTPGRDPREVREEEGIPELEDVRYYQPVVVKKVHGDHNFAEDSGGKYSKAAVDDPLK 300

Query: 301 VVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360
           VVNKNVAAAAYQTKPSA VED+VQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV
Sbjct: 301 VVNKNVAAAAYQTKPSATVEDSVQYEMGVVNKKLEGHGNGAPSSSAALKRGGGSRDVYEV 360

Query: 361 VKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVF 420
           VKEIEALFKKASEFGDEIAKMLE GQLPHQ KHA L RPPASR+RAKPSAMAGAAAEVVF
Sbjct: 361 VKEIEALFKKASEFGDEIAKMLEMGQLPHQRKHAFLARPPASRQRAKPSAMAGAAAEVVF 420

Query: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVE 480
           VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKR QLKRLHERGAEA+KVE
Sbjct: 421 VEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRRQLKRLHERGAEAKKVE 480

Query: 481 AIQTSINTL-------------------------------------TRMWKGMLECHHAQ 540
           AIQTSINTL                                     TRMWKGMLECHHAQ
Sbjct: 481 AIQTSINTLSTNLKIAIQVVDKISETINKIRDEELWPQVNELIQGLTRMWKGMLECHHAQ 540

Query: 541 FQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLL 600
           FQAIKES GLGHIRSGGKPSD DLRVTLQLDHELISWTTSFSGWISAQKNF RSLNNWLL
Sbjct: 541 FQAIKESHGLGHIRSGGKPSDFDLRVTLQLDHELISWTTSFSGWISAQKNFMRSLNNWLL 600

Query: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660
           KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI
Sbjct: 601 KCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQI 660

Query: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTISLQA 719
           WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGT SLQA
Sbjct: 661 WEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQALDKKLIMVTGHVQGDGTSSLQA 720

BLAST of Cp4.1LG16g03400 vs. ExPASy TrEMBL
Match: A0A6J1G762 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451484 PE=4 SV=1)

HSP 1 Score: 1085 bits (2806), Expect = 0.0
Identity = 588/796 (73.87%), Postives = 634/796 (79.65%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLDDL  V+LCR+RC FLDEAIRFR AFA+AH AYILSLQ VGKSLH+FIEPG+
Sbjct: 1   MGCATSKLDDLLVVSLCRERCNFLDEAIRFRQAFAEAHAAYILSLQGVGKSLHSFIEPGF 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYSEP SSP LKLP QRKGD DL+ SNSPLHRLSHSNS SHLHLPSDSDDDSSSLH SDH
Sbjct: 61  VYSEPFSSPKLKLPPQRKGDPDLEGSNSPLHRLSHSNSDSHLHLPSDSDDDSSSLHSSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGG------FVQMNYMKNNAMQSVVHQQMPISTER 180
           SSPLHQTH D  D+ DGNRGGFVSGGGG      +VQMNYM+N AM SVVHQQ  +S+ER
Sbjct: 121 SSPLHQTHDDRFDFRDGNRGGFVSGGGGGGGGGGYVQMNYMQNKAMPSVVHQQA-VSSER 180

Query: 181 VYQMGESASSSGYYPY------PNNGYSNYGGGYGGYYG--------------------- 240
           VY MGES+SSSGYYPY      PNNGY N GGGYGG YG                     
Sbjct: 181 VYHMGESSSSSGYYPYSYSNPYPNNGYPNNGGGYGGGYGGGYGGYGGYGGSPPTAYGGMS 240

Query: 241 -----SSSSKPPPPPPSPPRISAWDFLNFFETPAVGNYYGSYTPGRDPREVREEEGIPEL 300
                +SSSKPPPPPPSPPR SAWD  NFFETPAV NYYGSYTPGRDPREVR EEGIPEL
Sbjct: 241 NMLPPASSSKPPPPPPSPPRTSAWDIFNFFETPAVANYYGSYTPGRDPREVRAEEGIPEL 300

Query: 301 EDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDDPLKVVNKNVAAAAYQTKPSAAVEDA 360
           EDVRYYQPEVVKKVHG  N AVD GGK +   V+  +K V+KN+AA AYQTKPSAAV DA
Sbjct: 301 EDVRYYQPEVVKKVHGGQNSAVDGGGKQTNGTVNVQMKEVDKNLAALAYQTKPSAAVGDA 360

Query: 361 VQYEMGVVNKKLEGHGNGAPSSSAALKRGG-GSRDVYEVVKEIEALFKKASEFGDEIAKM 420
           V+    +V+K +   GNGAP++ AA   GG GSRDVYE V+EIEALFKKASEFGDEIAKM
Sbjct: 361 VR----LVDKNVMDDGNGAPAAVAAAFNGGAGSRDVYEAVREIEALFKKASEFGDEIAKM 420

Query: 421 LETGQLPHQHKHAILTRPPASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSATLKKLYMW 480
           LE G+LPHQ KHA   R PASRRRAK SA  G A E+VFVED+GMRSGNLS+TLKKLYMW
Sbjct: 421 LEMGELPHQRKHAFSARLPASRRRAKSSAKPGVA-EIVFVEDMGMRSGNLSSTLKKLYMW 480

Query: 481 EKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVEAIQTSINTL------------ 540
           EKKLY+EVK EEKMR++HE+KR QLKRLHERGAEA KVEA QTSI TL            
Sbjct: 481 EKKLYHEVKREEKMRLTHEQKRRQLKRLHERGAEATKVEATQTSIKTLSTSLNVAIQVVD 540

Query: 541 -------------------------TRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSD 600
                                    TRMWKGMLECHHAQFQA+KESRGLGHIRSGGKPSD
Sbjct: 541 KISETISKIRDEELWPQVNELIQGLTRMWKGMLECHHAQFQAVKESRGLGHIRSGGKPSD 600

Query: 601 LDLRVTLQLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGR 660
           LDLRVTLQLDHELISWT  FSGWISAQKNF RSLNNWLLKCLLYEPEET DG VPFSP R
Sbjct: 601 LDLRVTLQLDHELISWTARFSGWISAQKNFVRSLNNWLLKCLLYEPEETADGIVPFSPSR 660

Query: 661 IGAPPIFVICNQWSQVLDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERK 718
           IGAPPIFVICNQWSQ LDRFSEK+VVDSMHVFAKSVLQIW+HDKQE+R+TMITNKDLE+K
Sbjct: 661 IGAPPIFVICNQWSQGLDRFSEKEVVDSMHVFAKSVLQIWDHDKQELRQTMITNKDLEKK 720

BLAST of Cp4.1LG16g03400 vs. ExPASy TrEMBL
Match: A0A5A7V1D3 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003550 PE=4 SV=1)

HSP 1 Score: 1077 bits (2784), Expect = 0.0
Identity = 578/778 (74.29%), Postives = 627/778 (80.59%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLD LPAV+LCR+RCAFL+EAI+ RH FAQAHTAYILSLQ VGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDHLPAVSLCRERCAFLNEAIQHRHTFAQAHTAYILSLQGVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYS+PSSSP LK+P QRK D DL PSNSPLHRLSHSNS SHLHL SDSDDDSSSLHHSDH
Sbjct: 61  VYSDPSSSPKLKVPPQRKSDLDLDPSNSPLHRLSHSNSGSHLHLHSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLH TH DF DYPDGNRGG     GG+VQMNYMKNN++ SVVHQQMPI++ERVY MGE
Sbjct: 121 SSPLHPTHDDFFDYPDGNRGG-----GGYVQMNYMKNNSVPSVVHQQMPITSERVYHMGE 180

Query: 181 SASSSGY--YPYPNNGYSNYGGGYGG-YYGSS----------------SSKPPPPPPSPP 240
           S+SS  Y  YPY NNGYSNYGGGYGG YYGSS                SSKPPPPPPSPP
Sbjct: 181 SSSSGYYPSYPYSNNGYSNYGGGYGGGYYGSSPPSAYGGISNMLPSPSSSKPPPPPPSPP 240

Query: 241 RISAWDFLNFFETPAVGNYYGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHN 300
           R+S WDFLNFFETPAV NYYGSYTP RDPREVR EEGIPELEDVRY+QPEVVKKV+G+  
Sbjct: 241 RVSTWDFLNFFETPAVVNYYGSYTPSRDPREVRAEEGIPELEDVRYHQPEVVKKVNGEQK 300

Query: 301 FAVDSGGKYSKAAVDDPLKVVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNK--KLEGHGN 360
           F  D G K+ KA VDD LK++NKNVAA  YQ KP+AA+++  +    VV+K  KLE HGN
Sbjct: 301 FIEDGGEKHLKAMVDDQLKLINKNVAAPPYQMKPNAAIDEFKK----VVDKDKKLEDHGN 360

Query: 361 GAPSSSAALKRGGG--SRDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILT 420
           GAP+  A +K GGG  SRD+Y+  +EIE LFKKASEFGDEIAKMLE GQLPHQ KHA L 
Sbjct: 361 GAPAIGATMKGGGGGTSRDIYKAAREIEVLFKKASEFGDEIAKMLEMGQLPHQRKHAFLA 420

Query: 421 RPPASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRM 480
           RPPA+RRR K S+ AGAA EVVFVED+GM+SGNLS+TLKKLYMWEKKLYNEVK EEKMRM
Sbjct: 421 RPPATRRRVKSSSKAGAA-EVVFVEDMGMKSGNLSSTLKKLYMWEKKLYNEVKTEEKMRM 480

Query: 481 SHERKRHQLKRLHERGAEAQKVEAIQTSINTL---------------------------- 540
           +H+RKRHQLKRLHERGAEAQK+EA QTSINTL                            
Sbjct: 481 THDRKRHQLKRLHERGAEAQKIEATQTSINTLSTNLKIAIQVVDKISETINKIRDEELWP 540

Query: 541 ---------TRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISW 600
                    TRMWKGMLECHH QFQ IKES  +GH RS G PSD+DLRVTLQLDHELISW
Sbjct: 541 QVSELIQGLTRMWKGMLECHHDQFQVIKESCDIGHTRSCGNPSDMDLRVTLQLDHELISW 600

Query: 601 TTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQV 660
           TTSFSGWISAQKNF RSLNNWLLKCLLYEPEET DG VPFSP RIGAPPIFVICNQWSQ 
Sbjct: 601 TTSFSGWISAQKNFVRSLNNWLLKCLLYEPEETADGIVPFSPSRIGAPPIFVICNQWSQG 660

Query: 661 LDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQ 718
           LDRFSEKKVVDS+HV AKSVLQI E DKQEVR T ITNKDLE+K K+ DR+DQKLQKKIQ
Sbjct: 661 LDRFSEKKVVDSIHVAAKSVLQILERDKQEVRHTTITNKDLEKKVKKFDREDQKLQKKIQ 720

BLAST of Cp4.1LG16g03400 vs. ExPASy TrEMBL
Match: A0A5D3C0U4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G002280 PE=4 SV=1)

HSP 1 Score: 1075 bits (2779), Expect = 0.0
Identity = 577/778 (74.16%), Postives = 626/778 (80.46%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGCATSKLD LPAV+LCR+RCAFL+EAI+ RH FAQAHTAYILSLQ VGKSLHNFIEPGY
Sbjct: 1   MGCATSKLDHLPAVSLCRERCAFLNEAIQHRHTFAQAHTAYILSLQGVGKSLHNFIEPGY 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
           VYS+PSSSP LK+P QRK D DL PSNSPLHRLSHSNS SHLHL SDSDDDSSSLHHSDH
Sbjct: 61  VYSDPSSSPKLKVPPQRKSDLDLDPSNSPLHRLSHSNSGSHLHLHSDSDDDSSSLHHSDH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGE 180
           SSPLH TH DF DYPDGNRGG     GG+VQMNYMKNN++ SVVHQQMPI++ERVY MGE
Sbjct: 121 SSPLHPTHDDFFDYPDGNRGG-----GGYVQMNYMKNNSVPSVVHQQMPITSERVYHMGE 180

Query: 181 SASSSGY--YPYPNNGYSNYGGGYGG-YYGSS----------------SSKPPPPPPSPP 240
           S+SS  Y  YPY NNGYSNYGGGYGG YYGSS                SSKPPPPPPSPP
Sbjct: 181 SSSSGYYPSYPYSNNGYSNYGGGYGGGYYGSSPPSAYGGISNMLPSPSSSKPPPPPPSPP 240

Query: 241 RISAWDFLNFFETPAVGNYYGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHN 300
           R+S WDFLNFFETPAV NYYGSYTP RDPREVR EEGIPELEDVRY+QPEVVKKV+G+  
Sbjct: 241 RVSTWDFLNFFETPAVVNYYGSYTPSRDPREVRAEEGIPELEDVRYHQPEVVKKVNGEQK 300

Query: 301 FAVDSGGKYSKAAVDDPLKVVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNK--KLEGHGN 360
           F  D G K+ KA VDD LK++NKNVAA  YQ KP+AA+++  +    VV+K  KLE HGN
Sbjct: 301 FIEDGGEKHLKAMVDDQLKLINKNVAAPPYQMKPNAAIDEFKK----VVDKDKKLEDHGN 360

Query: 361 GAPSSSAALKRGGG--SRDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHAILT 420
           GAP+  A +K GGG  SRD+Y+  +EIE LFKKASEFGDEIAKMLE GQLPHQ KHA L 
Sbjct: 361 GAPAIGATMKGGGGGTSRDIYKAAREIEVLFKKASEFGDEIAKMLEMGQLPHQRKHAFLA 420

Query: 421 RPPASRRRAKPSAMAGAAAEVVFVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRM 480
           RPPA+RRR K S+ AGAA EVVFVED+GM+SGNLS+TLKKLYMWEKKLYNEVK EEKMRM
Sbjct: 421 RPPATRRRVKSSSKAGAA-EVVFVEDMGMKSGNLSSTLKKLYMWEKKLYNEVKTEEKMRM 480

Query: 481 SHERKRHQLKRLHERGAEAQKVEAIQTSINTL---------------------------- 540
           +H+RKRHQLKRLHERGAEAQK+EA QTSINTL                            
Sbjct: 481 THDRKRHQLKRLHERGAEAQKIEATQTSINTLSTNLKIAIQVVDKISETINKIRDEELWP 540

Query: 541 ---------TRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISW 600
                    TRMWKGMLECHH QFQ IKES  +GH RS G PSD+DLRVTLQLDHELISW
Sbjct: 541 QVSELIQGLTRMWKGMLECHHDQFQVIKESCDIGHTRSCGNPSDMDLRVTLQLDHELISW 600

Query: 601 TTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQWSQV 660
           TT FSGWISAQKNF RSLNNWLLKCLLYEPEET DG VPFSP RIGAPPIFVICNQWSQ 
Sbjct: 601 TTCFSGWISAQKNFVRSLNNWLLKCLLYEPEETADGIVPFSPSRIGAPPIFVICNQWSQG 660

Query: 661 LDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRDDQKLQKKIQ 718
           LDRFSEKKVVDS+HV AKSVLQI E DKQEVR T ITNKDLE+K K+ DR+DQKLQKKIQ
Sbjct: 661 LDRFSEKKVVDSIHVAAKSVLQILERDKQEVRHTTITNKDLEKKVKKFDREDQKLQKKIQ 720

BLAST of Cp4.1LG16g03400 vs. TAIR 10
Match: AT4G35240.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 587.8 bits (1514), Expect = 1.2e-167
Identity = 398/853 (46.66%), Postives = 508/853 (59.55%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGC +SKLDDLPAVALCR+RCAFL+ AI  R+A A++H AY  SL+ +G SLH FI   +
Sbjct: 1   MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLHLFINHHH 60

Query: 61  VY-----SEPSSSPNLKLPNQRKGDSDLQPSNSPL----------HRLSHSNS---SSHL 120
            +     +    SP L LP QRKGD D + +NSP           H  +HS S   S HL
Sbjct: 61  RFVASGGANVGDSPRLNLPPQRKGDLDDEATNSPKKQKLASSHHNHNHAHSGSGSDSGHL 120

Query: 121 HLPSDSDDDS--------SSLHHSDHSSPLH------------------QTHGDFLDYPD 180
              SDSD+D          SLHH  HS P H                  Q  G    YP+
Sbjct: 121 EFDSDSDEDEEDDDDLDLDSLHH--HSPPHHHLGNFPIPESAPMGGYMEQQPGYINPYPN 180

Query: 181 GNRGGF-VSGGGGFVQMNYMKNNAM-QSVVHQQMPISTERVYQMGESASSSGYYPYPNN- 240
            +  G   SGGG ++ MNYMKN +M  SVV++Q P S +RVY +GES+SS   YPYP   
Sbjct: 181 PDMMGHPYSGGGSYMHMNYMKNKSMPPSVVYEQRPTSPQRVY-IGESSSS---YPYPPQN 240

Query: 241 ---GYSNYGGGYG-GYYGSSS-------SKPPPPPPSPPRISAWDFLNFFETPAVGNYYG 300
              GYSN   G G GYYGSSS       +KPPPPPPSPPR + WDFLN F+T     YY 
Sbjct: 241 SYFGYSNPVPGPGPGYYGSSSASTTAAATKPPPPPPSPPRSNGWDFLNPFDT-----YYP 300

Query: 301 SYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAV-----DD 360
            YTP RD RE+REEEGIP+LED   +  EVVK+V+G   FA   G + + AAV     + 
Sbjct: 301 PYTPSRDSRELREEEGIPDLEDDDSHY-EVVKEVYGKPKFAAGGGHQPNPAAVHMMREES 360

Query: 361 PLKVVNKNVA----------AAAYQTKPSAAVE-DAVQYEMGVVNKKL----EGHGNGAP 420
           P   ++K+ A          A+AYQ++PS +VE + ++YE+ VV KK+    E   N   
Sbjct: 361 PSPPLDKSGASTSGGGDVGDASAYQSRPSVSVEKEGMEYEVHVVEKKVVEDEERRSNATA 420

Query: 421 SSSAALKRGGGSRDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHA---ILTRP 480
           +       GGG R V EV KEIE  F KA+E G EIAK+LE G+ P+  KHA   +L   
Sbjct: 421 TRGGG--GGGGPRAVPEVAKEIENQFVKAAESGSEIAKLLEVGKHPYGRKHAASKMLHGV 480

Query: 481 PASRRRAKPSAMAGAAAEVV------FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEE 540
             S         + AAA VV        E++  RS NLS+TL KL++WEKKLY+EVK EE
Sbjct: 481 TPSLPSTSGGTSSSAAAAVVPPTYADIEEELASRSRNLSSTLHKLHLWEKKLYHEVKAEE 540

Query: 541 KMRMSHERKRHQLKRLHERGAEA---------------------QKVEAIQTSINT---- 600
           K+R++HE+K  +LKRL +RGAEA                     Q V+ I  +IN     
Sbjct: 541 KLRLAHEKKLRKLKRLDQRGAEAIKVDKTRKLVRDMSTKIRIAIQVVDKISVTINKIRDE 600

Query: 601 ------------LTRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHE 660
                       LTRMWK MLECH +Q QAI+E++GLG IR+  K  D  L  T  L HE
Sbjct: 601 DLWPQLNALIQGLTRMWKTMLECHQSQCQAIREAQGLGPIRASKKLGDEHLEATSLLGHE 660

Query: 661 LISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQ 716
           LI+W   FS W+SAQK + + LN WL+KCLLYEPEETPDG VPFSPGRIGAPPIFVICNQ
Sbjct: 661 LINWILGFSSWVSAQKGYVKELNKWLMKCLLYEPEETPDGIVPFSPGRIGAPPIFVICNQ 720

BLAST of Cp4.1LG16g03400 vs. TAIR 10
Match: AT4G35240.2 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 587.8 bits (1514), Expect = 1.2e-167
Identity = 398/853 (46.66%), Postives = 508/853 (59.55%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGC +SKLDDLPAVALCR+RCAFL+ AI  R+A A++H AY  SL+ +G SLH FI   +
Sbjct: 1   MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLHLFINHHH 60

Query: 61  VY-----SEPSSSPNLKLPNQRKGDSDLQPSNSPL----------HRLSHSNS---SSHL 120
            +     +    SP L LP QRKGD D + +NSP           H  +HS S   S HL
Sbjct: 61  RFVASGGANVGDSPRLNLPPQRKGDLDDEATNSPKKQKLASSHHNHNHAHSGSGSDSGHL 120

Query: 121 HLPSDSDDDS--------SSLHHSDHSSPLH------------------QTHGDFLDYPD 180
              SDSD+D          SLHH  HS P H                  Q  G    YP+
Sbjct: 121 EFDSDSDEDEEDDDDLDLDSLHH--HSPPHHHLGNFPIPESAPMGGYMEQQPGYINPYPN 180

Query: 181 GNRGGF-VSGGGGFVQMNYMKNNAM-QSVVHQQMPISTERVYQMGESASSSGYYPYPNN- 240
            +  G   SGGG ++ MNYMKN +M  SVV++Q P S +RVY +GES+SS   YPYP   
Sbjct: 181 PDMMGHPYSGGGSYMHMNYMKNKSMPPSVVYEQRPTSPQRVY-IGESSSS---YPYPPQN 240

Query: 241 ---GYSNYGGGYG-GYYGSSS-------SKPPPPPPSPPRISAWDFLNFFETPAVGNYYG 300
              GYSN   G G GYYGSSS       +KPPPPPPSPPR + WDFLN F+T     YY 
Sbjct: 241 SYFGYSNPVPGPGPGYYGSSSASTTAAATKPPPPPPSPPRSNGWDFLNPFDT-----YYP 300

Query: 301 SYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAV-----DD 360
            YTP RD RE+REEEGIP+LED   +  EVVK+V+G   FA   G + + AAV     + 
Sbjct: 301 PYTPSRDSRELREEEGIPDLEDDDSHY-EVVKEVYGKPKFAAGGGHQPNPAAVHMMREES 360

Query: 361 PLKVVNKNVA----------AAAYQTKPSAAVE-DAVQYEMGVVNKKL----EGHGNGAP 420
           P   ++K+ A          A+AYQ++PS +VE + ++YE+ VV KK+    E   N   
Sbjct: 361 PSPPLDKSGASTSGGGDVGDASAYQSRPSVSVEKEGMEYEVHVVEKKVVEDEERRSNATA 420

Query: 421 SSSAALKRGGGSRDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHA---ILTRP 480
           +       GGG R V EV KEIE  F KA+E G EIAK+LE G+ P+  KHA   +L   
Sbjct: 421 TRGGG--GGGGPRAVPEVAKEIENQFVKAAESGSEIAKLLEVGKHPYGRKHAASKMLHGV 480

Query: 481 PASRRRAKPSAMAGAAAEVV------FVEDIGMRSGNLSATLKKLYMWEKKLYNEVKGEE 540
             S         + AAA VV        E++  RS NLS+TL KL++WEKKLY+EVK EE
Sbjct: 481 TPSLPSTSGGTSSSAAAAVVPPTYADIEEELASRSRNLSSTLHKLHLWEKKLYHEVKAEE 540

Query: 541 KMRMSHERKRHQLKRLHERGAEA---------------------QKVEAIQTSINT---- 600
           K+R++HE+K  +LKRL +RGAEA                     Q V+ I  +IN     
Sbjct: 541 KLRLAHEKKLRKLKRLDQRGAEAIKVDKTRKLVRDMSTKIRIAIQVVDKISVTINKIRDE 600

Query: 601 ------------LTRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHE 660
                       LTRMWK MLECH +Q QAI+E++GLG IR+  K  D  L  T  L HE
Sbjct: 601 DLWPQLNALIQGLTRMWKTMLECHQSQCQAIREAQGLGPIRASKKLGDEHLEATSLLGHE 660

Query: 661 LISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIFVICNQ 716
           LI+W   FS W+SAQK + + LN WL+KCLLYEPEETPDG VPFSPGRIGAPPIFVICNQ
Sbjct: 661 LINWILGFSSWVSAQKGYVKELNKWLMKCLLYEPEETPDGIVPFSPGRIGAPPIFVICNQ 720

BLAST of Cp4.1LG16g03400 vs. TAIR 10
Match: AT2G17110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 515.0 bits (1325), Expect = 9.7e-146
Identity = 338/793 (42.62%), Postives = 455/793 (57.38%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGC+TSKLDDLPAVALCRDRC+FL+ AI  R+A ++AH +Y  SL+A+  SLH FI   +
Sbjct: 1   MGCSTSKLDDLPAVALCRDRCSFLEAAIHQRYALSEAHVSYTQSLKAISHSLHQFINHHH 60

Query: 61  VYSEPSSSPNLKLPNQRKGDSDLQPSNSPLHRLSHSNSSSHLHLPSDSDDDSSSLHHSDH 120
            Y++ S SP    P    G                   S HL   SDSD D      S H
Sbjct: 61  RYND-SDSPKKAKPKMDSG-------------------SGHLDFDSDSDSDDDDDIDSLH 120

Query: 121 SSPLHQTHGDFLDYPDGNRGGFVSGGGGFVQMNYMKNNAM-QSVVHQQMPISTERVYQMG 180
           SSPLH      L+  D N          ++ MNYMKN+ M  S+V++Q P S +RV+  G
Sbjct: 121 SSPLHH----HLEDDDSN-------PKSYLHMNYMKNSYMPPSLVYEQRPSSPQRVH-FG 180

Query: 181 ESASS--SGYYPYPNNGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAV 240
           ES+SS  S Y PY N   SNYG           SK PPPPPSPPR   WDFL+ F+T   
Sbjct: 181 ESSSSSTSEYNPYLN---SNYG-----------SKLPPPPPSPPREKVWDFLDPFDT--- 240

Query: 241 GNYYGSYTPGRDPREVREEEGIPELEDVRYYQPEVVKKVHGDHNFAVDSGGKYSKAAVDD 300
             YY  YTP RD RE+R+E G+P+LE+       VVK+VHG   F        +  +V++
Sbjct: 241 --YYTPYTPSRDTRELRDELGVPDLEE----DDVVVKEVHGKQKFV-------AAVSVEE 300

Query: 301 PLKVVNKNVA--------AAAYQTKPSAAVE-DAVQYEMGVVNKKL--EGHGNGAPSSSA 360
           PL     + +        A+ YQT+PS +VE + +++E+ +V KK+  +  G+    S A
Sbjct: 301 PLGNSGASTSGGGGGGGKASLYQTRPSVSVEKEEMEHEVHIVEKKIVEDSGGDEVRKSKA 360

Query: 361 ALKRGGGS--RDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKH----------- 420
           A+ RGGG   R V EV KEIEA F +A+E G+EIA MLE G+ P+  K+           
Sbjct: 361 AVARGGGGVRRGVPEVAKEIEAQFLRAAESGNEIAVMLEVGKHPYGRKNVSSKKLYEGTP 420

Query: 421 ---AILTRPPASRRRAKPSAMAGAAAEVV--FVEDIGMRSGNLSATLKKLYMWEKKLYNE 480
               + +   ++ ++AK  A +   A        ++ ++S NLS+TL KL++WEKKLY+E
Sbjct: 421 SPSVVSSAQSSTSKKAKAEASSSVTAPTYADIEAELALKSRNLSSTLHKLHLWEKKLYDE 480

Query: 481 VKGEEKMRMSHERKRHQLKRLHERGAEAQKVEA---------------------IQTSIN 540
           VK EEKMR++HE+K  +LKR+ ERGAE QKV++                     I  +IN
Sbjct: 481 VKAEEKMRVNHEKKLRKLKRMDERGAENQKVDSTRKLVRSLSTKIRIAIQVVDKISVTIN 540

Query: 541 T----------------LTRMWKGMLECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTL 600
                            L++MWK MLECH +Q +AIKE+RGLG IR+        L VT 
Sbjct: 541 KIRDEELWLQLNELIQGLSKMWKSMLECHKSQCEAIKEARGLGPIRASKNFGGEHLEVTR 600

Query: 601 QLDHELISWTTSFSGWISAQKNFTRSLNNWLLKCLLYEPEETPDGFVPFSPGRIGAPPIF 660
            L +ELI+W   FS W+SAQK F R LN+WL+KCL YEPEETPDG VPFSPGRIGAP IF
Sbjct: 601 TLGYELINWIVGFSSWVSAQKGFVRELNSWLMKCLFYEPEETPDGIVPFSPGRIGAPMIF 660

Query: 661 VICNQWSQVLDRFSEKKVVDSMHVFAKSVLQIWEHDKQEVRKTMITNKDLERKAKRIDRD 719
           VICNQW Q LDR SEK+V++++  F  SVL +WE D+   R+ +I + D     + +DR+
Sbjct: 661 VICNQWEQALDRISEKEVIEAIRRFTTSVLHLWEQDRLATRERIIGHGD----PRNMDRE 720

BLAST of Cp4.1LG16g03400 vs. TAIR 10
Match: AT1G77500.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 303.1 bits (775), Expect = 5.8e-82
Identity = 275/886 (31.04%), Postives = 392/886 (44.24%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIE--- 60
           MGC  SK+D+ P V LCR+R   L  A   R A A AH  Y  SL  VG+++  F++   
Sbjct: 1   MGCGGSKVDNQPIVILCRERKELLKAASYHRSALAVAHLTYFQSLSDVGEAIQRFVDDEV 60

Query: 61  -PGYVYSEPSSSPNLKLP-NQRKGDSDLQPSNSPLHRLSHS-------NSSSHLHLPSDS 120
             G+  S    SP L LP ++ K     + S S    +SHS       +  SHLHL S S
Sbjct: 61  VAGFSSSSSPDSPVLTLPSDEGKPTKHKRISPSSTTSISHSVIEEEDTDDDSHLHLSSGS 120

Query: 121 DDDS------------------------SSLHHSDHSSPLH---QTHGDFLDYPDGNRGG 180
           + +S                        S  H ++++ P++      G    YP G   G
Sbjct: 121 ESESEVGSKDHIQITSTPEQERSTESFPSGYHPTNYAPPVYPPGYPPGYPFSYPTGYSSG 180

Query: 181 FVSGG---------GGFVQMNYMKNNAMQSVVHQQMPISTERVYQMGESASSSGYYPYPN 240
               G          G   M YMK +A QS      P    RV +  +  S SG   + N
Sbjct: 181 NYQPGYPNYTGENPYGNRGMYYMKKSAPQSRPFIFQP-ENHRVEENAQWPSDSG---FRN 240

Query: 241 NGYSNYGGGYGGYYGSSSSKPPPPPPSPPRISAWDFLNFFETPAVGN-------YY---- 300
            G                S  P PPPSPP +S WDFLN F+T    N       YY    
Sbjct: 241 TGVQR------------RSPSPLPPPSPPTVSTWDFLNVFDTYDYSNARSRASGYYPMGM 300

Query: 301 GSYTPGRDPREVREEEGIPELEDVR--------YYQP-----EVVKKVHGDHNFAVDSGG 360
            S +   D +EVRE EGIPELE+V         Y +P     E VK+   +H   V    
Sbjct: 301 ASISSSPDSKEVREREGIPELEEVTEQEVIKQVYRRPKRPGLEKVKEHRDEHKHNVFPER 360

Query: 361 KYSKAAVDDPLKVVNKNVAAAAYQTKPSAAVEDAVQYEMGVVNKKLE----GHGNGAPSS 420
             +K  V  P +V   ++ +    +   + VE    Y  G   K        HG G  SS
Sbjct: 361 NINKREVPMPEQVTESSLDSETISSFSGSDVESEFHYVNGGEGKSSSISSISHGAGTKSS 420

Query: 421 SAALKRGG----------------------------------GSRDVYEVVKEIEALFKK 480
               ++ G                                   +RD+ EVVKEI++ F+ 
Sbjct: 421 REVEEQYGRKKGVSFELEETTSTSSFDVESSKISSLSSLSIHATRDLREVVKEIKSEFEI 480

Query: 481 ASEFGDEIAKMLETGQLPHQHKH----AILTR------PPASRRRAKP------SAMAGA 540
           AS  G E+A +LE G+LP+QHK+     IL+R      P      ++P      ++    
Sbjct: 481 ASSCGKEVALLLEVGKLPYQHKNNGVKVILSRIMYLVAPSTRSSHSQPRLSIRLTSRTRK 540

Query: 541 AAEVVFVEDI-GMRSGNLSATLKKLYMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERG 600
            A+    +D+ G  +GNLS+TL+KLY WEKKLY EVK EEK+R  +E K  +LK++   G
Sbjct: 541 MAKSYNGQDVNGGFNGNLSSTLEKLYAWEKKLYKEVKDEEKLRAIYEEKCRRLKKMDSHG 600

Query: 601 AEAQKVEAIQTS-------------------------------------INTLTRMWKGM 660
           AE+ K++A + +                                     I+ L RMW+ M
Sbjct: 601 AESIKIDATRAAIRKLLTKIDVCIRSVDSISSRIHKLRDEELQPQLIQLIHGLIRMWRSM 660

Query: 661 LECHHAQFQAIKESRGLGHIRSGGKPSDLDLRVTLQLDHELISWTTSFSGWISAQKNFTR 705
           L CH  QFQAI+ES+      +    +D      L L+ EL  W  SF+ W++ QK++ +
Sbjct: 661 LRCHQKQFQAIRESKVRSLKANTTLQNDSGSTAILDLEIELREWCISFNNWVNTQKSYVQ 720

BLAST of Cp4.1LG16g03400 vs. TAIR 10
Match: AT1G21740.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 280.8 bits (717), Expect = 3.1e-75
Identity = 282/947 (29.78%), Postives = 399/947 (42.13%), Query Frame = 0

Query: 1   MGCATSKLDDLPAVALCRDRCAFLDEAIRFRHAFAQAHTAYILSLQAVGKSLHNFIEPGY 60
           MGC  SK+DD P V LCR+R   +  A   R A A AH +Y  SL  VG S+  F++   
Sbjct: 1   MGCGGSKVDDQPLVILCRERKQLIKAASHHRCALAAAHLSYFQSLCDVGDSIKRFVDEEL 60

Query: 61  VYSEPSS-----SPNLKLPNQRKGDSDLQPSNSPLHRLSHS---------NSSSHLHLPS 120
           V    SS     SP L LP+  +G       +S    +SHS             HLHL S
Sbjct: 61  VLVGTSSSSSPDSPVLTLPSD-EGKPHKHKISSSSTSVSHSVIEDDDEGEGEDQHLHLSS 120

Query: 121 DSDDDSSSLHHSDHS------------------SPLHQTHGDFLDYPDGNRGGFVSG--- 180
            S+ DS S   SD S                   P +   G    Y  G + GF  G   
Sbjct: 121 GSELDSGSESGSDDSLGHIHIETSPKVKEKETRLPENYQPGYQSGYQSGYQPGFTPGYQY 180

Query: 181 -------------GGGFVQMN-----YMKNNAMQSVVHQQMPISTER--VYQMGESASSS 240
                        G GF+  N     Y   N    +   +  +   R  V+Q       +
Sbjct: 181 QPGYSAGYQYPVEGWGFMGENPNLNPYPNPNQNPGMYFMKKSVQPSRPVVFQPENHRVEN 240

Query: 241 G-YYPYPNNGYSNY--GGGYGGYYG--------SSSSKPPPPPPSPPRISAWDFLNFFET 300
           G + P    GYSNY  G    GY+G         S  +P P PPSPPRIS+WDFLN F+T
Sbjct: 241 GQWLPENGVGYSNYYPGNANTGYFGYPEQRREPPSPVRPTPAPPSPPRISSWDFLNVFDT 300

Query: 301 -------------------PAVGNYYGSYTPGRDPREVREEEGIPELED----------- 360
                              PA+G    S +   D REVRE EGIPELE+           
Sbjct: 301 YDYNRAGGGESSGAGAGFFPAMGG-GKSNSSSPDSREVREREGIPELEEETEQEVIFGQT 360

Query: 361 VRYYQPEVVKKVHGDH----------------------------------NFAVDSGGKY 420
            ++ + + ++KV   H                                  +F   +   +
Sbjct: 361 FKHMKRKGIEKVKEQHRQENEIHERKIKKRGDSGEGTSRAVPVVERATESSFGSKTVSSF 420

Query: 421 SKAAVDDPLKVVNKNVAAAA---------YQTKPSAAVEDAVQYEMGV-----VNKKLEG 480
           S +  +     VN     ++           TK    VE+    + GV      N     
Sbjct: 421 SSSEEESEFHHVNDGEGKSSSNDLGGHETVATKSVGEVEEEYVRKKGVSFELDENVTTSF 480

Query: 481 HGNGAPSSSAALKRGGGSRDVYEVVKEIEALFKKASEFGDEIAKMLETGQLPHQHKHA-- 540
               +  SS +      +RD+ EVVKEI++ F+ AS  G E+A +LE  +LP+Q K +  
Sbjct: 481 DVESSKISSLSALSVHATRDLREVVKEIKSEFEVASSHGKEVAVLLEVSKLPYQQKSSGL 540

Query: 541 --------ILTRPPASRRRAKPSAMAGAAAEVVFV------EDI--GMRSGNLSATLKKL 600
                    L  P     R++P       + ++ +      +D+  G+ +GNLSATL++L
Sbjct: 541 KVIFSRIMYLVAPSTVSSRSQPQPSIRLTSRILKIAKSYNGQDVREGL-TGNLSATLEQL 600

Query: 601 YMWEKKLYNEVKGEEKMRMSHERKRHQLKRLHERGAEAQKVEAIQTS------------- 660
           Y WEKKLY EVK EEK+R+ +E K   LK+L   GAE+ K++  + +             
Sbjct: 601 YAWEKKLYKEVKDEEKLRVVYEEKCRTLKKLDSLGAESSKIDTTRAAIRKLLTKLDVCIR 660

Query: 661 ------------------------INTLTRMWKGMLECHHAQFQAIKESRGLGHIRSGGK 707
                                   I+ L RMW+ ML+CH  QFQAI ES+      + G 
Sbjct: 661 SVDSISSRIHKLRDEELQPQLTQLIHGLIRMWRSMLKCHQKQFQAIMESKVRSLRANTGL 720