Cp4.1LG16g03170 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g03170 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 1.1e-58
Identity = 195/711 (27.43%), Postives = 341/711 (47.96%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHS 60
           MGC  S+++ +E+VS C+ RKR++K  V+ R  L+ +H+ Y ++L AV +++  F    S
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHF---SS 60

Query: 61  SPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTES 120
             +P  +   P   S  PPP             RP        P  P    ++ +T+T S
Sbjct: 61  KETPLHLHHNPPSPSPPPPP-----------PPRPPPP-----PLSPGSETTTWTTTTTS 120

Query: 121 SMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQSS 180
           S+                        PPP P P    +WDF++PF       +     SS
Sbjct: 121 SV-------------------LPPPPPPPPPPPPPSSTWDFWDPF-------IPPPPSSS 180

Query: 181 EEEEGIPELEEETVEKNQRVEDVG-----------------------EKEEENQLKKGLK 240
           EE     E EEET    +     G                        K+       G +
Sbjct: 181 EE-----EWEEETTTATRTATGTGSDAAVTTAPTTATPQASSVVSGFSKDTMTTTTTGSE 240

Query: 241 V-IDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQ 300
           + +     G++L+E +++V++YF++A DSG  +S +LE     + + + +   +S     
Sbjct: 241 LAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLE-----ISTSITDFSGHSK---- 300

Query: 301 AITWHRSVSGKTSSCKSLVASSSKGSSPWTE-FSNELFDDYDIMD--------AGSHSST 360
                   SGK  S  +   + +  +S WT  F+     +Y             GSHSST
Sbjct: 301 --------SGKMYSSSNYECNLNP-TSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSHSST 360

Query: 361 LGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARIL 420
           + RLYAWEKKLY+EVK  +S+   +EK+  ++R  ++K +  + T+K +  V+ L +++ 
Sbjct: 361 VDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQLS 420

Query: 421 VAIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHS 480
           V+ ++ +S S  I+ LR+ EL PQ+VEL+KGL   W+ M E H+ Q  I+ ++K  +   
Sbjct: 421 VSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLNTIP 480

Query: 481 YLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQ 540
             +  +E HR +TLQL  E+Q W   F   V++Q+ Y+++L GWL           S  Q
Sbjct: 481 STEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLR---------LSLFQ 540

Query: 541 TSAVPC--GLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKR 600
            S  P     +   + S C +W  +++++PDK  +  +KSF   +  +  +Q +E +QK+
Sbjct: 541 FSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKK 600

Query: 601 RVESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDL 660
           R ES+ K+ +++  S +  E+K+  ++  E++ +  V        EK+ +++M + K + 
Sbjct: 601 RTESMLKDFEKKSASLRALESKYSPYSVPESRKKNPV-------IEKRVKVEMLKGKAEE 627

Query: 661 EKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSEN 677
           EK KH   +   + +TLN +Q GF  VF ++  FS    + ++ + N +++
Sbjct: 661 EKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKS 627

BLAST of Cp4.1LG16g03170 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.7e-54
Identity = 211/778 (27.12%), Postives = 346/778 (44.47%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHS 60
           MGC ASK+E+E+ V  C+ER+RH+K AV  R  LA AH+ Y ++L   +AA+  F   H 
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  S------PSPFLITF------------PP--TCSSSSPPPSEKVISNPMFLQQRPSDSTH 120
           S       +P L+T             PP  T SSS PPP+  +  +       P   +H
Sbjct: 61  SLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQAPPPPPPTQSH 120

Query: 121 EAIPACPSCACSS-----------SSTSTESSMEEREEEPVEEERIEQFPAYFYMQMPPP 180
           +  P     A              S +S  S       +PV         A+ +    PP
Sbjct: 121 QPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTP-SSSSAWDWENFYPP 180

Query: 181 MPSPQREFSWDFFNPFDTMRTDV--VGGFRQSSEEEEGIPELEEETVEKNQRVEDVGEKE 240
            P P  EF       FD  + D+      R+  EEE+    L    +++   V+D  ++ 
Sbjct: 181 SP-PDSEF-------FDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDDER 240

Query: 241 EENQLKKGLKVID----------------------------------------------- 300
           EE     G +  D                                               
Sbjct: 241 EEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPLPL 300

Query: 301 -------------------------TPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEA 360
                                      +  R L E +  +E+YF++A ++G  VS +LEA
Sbjct: 301 PLRRRDERSEAGDSSSTVTAAAEMRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSELLEA 360

Query: 361 NKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDD 420
           ++ QL     ++K+          +H   +   SS  S   S    +  +   +N L  +
Sbjct: 361 SRAQLDRNFRQLKKT--------VYHS--NSLLSSLSSTWTSKPPLAVRYKLDTNAL--E 420

Query: 421 YDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKT 480
            + M+  SH STL RL AWEKKLY+EVKA +S+   +EK+ S L++ + +G +    DKT
Sbjct: 421 MESMEGKSHGSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKT 480

Query: 481 RVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKK 540
           + ++  L + I+V  ++A + S+ IV +RD+EL PQ+VEL   L   W+ M   HE Q +
Sbjct: 481 KASINKLQSLIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNE 540

Query: 541 IILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKF 600
           I+ +V+    +S  +  ++ HRLAT  L A +  W S F++ ++ Q+ Y+ AL+GWL K 
Sbjct: 541 IVQQVRGLVDNSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWL-KL 600

Query: 601 VVPEVEFCSRGQTSAVP----CGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMK 660
            + +V+       S +P      L    L + C +W  ++++LPD   + A+KSF   + 
Sbjct: 601 TLFQVD-------SNIPQEAYTSLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVH 660

BLAST of Cp4.1LG16g03170 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 2.8e-49
Identity = 215/812 (26.48%), Postives = 349/812 (42.98%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVA--- 60
           MGC ASKL+ E+ V  C++R+R +K AV  R+ LA AH+ YC++L    +A+  F +   
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---RHSSPSPFLITFPPTCSSSSP----PPSEKVISNPMFLQQRPSDSTHEAIPACPSCA 120
                 +P+ FL T PP  S  SP    PP       P  +   PS S   A    PS  
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYP-PSTSPSVASSKQPSVM 120

Query: 121 CSSSSTSTESSMEER------EEEPVEEERIEQ-------FPAYF----YMQMP------ 180
            +SS+   +   + R      E  P    R E+       +P+ +    Y   P      
Sbjct: 121 STSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSV 180

Query: 181 -------PPMPSPQREF--------------------------SWDFFN-------PFDT 240
                  PP P P  EF                           +DFF+        F++
Sbjct: 181 WNWENFYPPSP-PDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFES 240

Query: 241 MRTDVVGGFRQSSEEEEGIPELEEETVEKNQRVEDVGEKEEENQLKKGLKVIDT------ 300
           MR  V     ++  EE    E E+          D  E+EEE+  ++ +  + T      
Sbjct: 241 MRNQVEEE-TETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGS 300

Query: 301 -----------------------------------------------------PVEGREL 360
                                                                 V  R+L
Sbjct: 301 TVRSNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRHRDL 360

Query: 361 LEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKT 420
            E +  +++ F +A  SG +VS+MLE  + +L     ++K                    
Sbjct: 361 KEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLK-------------------- 420

Query: 421 SSCKSLVASS---SKGSSPWTE---FSNELFDDYDIMDAGSHS----STLGRLYAWEKKL 480
              K+++ SS   S  SS WT     + +   D   +D  + S    STL RL AWEKKL
Sbjct: 421 ---KTVIHSSSLLSNLSSTWTSKPPLAVKYRIDTTALDQPNSSKSLCSTLDRLLAWEKKL 480

Query: 481 YEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESIST 540
           YEE+KA +     +EK+ S+L++Q+ KG +    DKT+ ++  L + I+V  ++  + ST
Sbjct: 481 YEEIKAREGFKIEHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTST 540

Query: 541 RIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYL-KFCNESHR 600
            I+ LRD +L PQ+VEL  G    WK M + HETQ  I+ +V+     S   +  +E HR
Sbjct: 541 AIIRLRDTDLVPQLVELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHR 600

Query: 601 LATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHG 660
            AT  L + + +W S FS  ++ Q+ ++ ++H W    ++P    C     +      H 
Sbjct: 601 QATRDLESAVSSWHSSFSSLIKFQRDFIHSVHAWFKLTLLP---VCQEDAANH-----HK 660

BLAST of Cp4.1LG16g03170 vs. NCBI nr
Match: KAG7010840.1 (hypothetical protein SDJN02_27636, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1300 bits (3363), Expect = 0.0
Identity = 681/700 (97.29%), Postives = 691/700 (98.71%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SSMEEREEEPVEEERIEQ PAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV G+ QS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180

Query: 181 SEEEEGIPELEEETVEKNQRVEDVGEKEEENQLKKG-LKVIDTPVEGRELLEALQDVEDY 240
           SEEEEG+PELEEETVEKNQRVED+GEKEEE+QLKK  L+VIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGI+VSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMD+GSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420
           LRNQDVKGSNGMTTDKTRV VKDLYARILVAIRSAESISTRIVNLRDDELQPQI+ELLKG
Sbjct: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCS+G+TSAVP GLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANNSKNINYIEGSSQTEENVSR 698
           FSKASQKMYDHLVNYSENAN SKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Cp4.1LG16g03170 vs. NCBI nr
Match: KAG6571010.1 (Protein ROLLING AND ERECT LEAF 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1295 bits (3350), Expect = 0.0
Identity = 678/699 (97.00%), Postives = 689/699 (98.57%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SSMEEREEEPVEEERIEQ PAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV G+ QS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVSGYHQS 180

Query: 181 SEEEEGIPELEEETVEKNQRVEDVGEKEEENQLKKG-LKVIDTPVEGRELLEALQDVEDY 240
           SEEEEG+PELEEETVEKNQRVED+GEKEEE+QLKK  L+VIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGMPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGI+VSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMD+GSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420
           LRNQDVKGSNGMTTDKTRV VKDLYARILVAIRSAESISTRIVNLRDDELQPQI+ELLKG
Sbjct: 361 LRNQDVKGSNGMTTDKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIIELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCS+G+TSAVP GLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSQGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKEL+RRILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELERRILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANNSKNINYIEGSSQTEENVS 697
           FSKASQKMYDHLVNYSENAN SKNINYIEGSSQTEEN S
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENTS 699

BLAST of Cp4.1LG16g03170 vs. NCBI nr
Match: XP_022944061.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 679/700 (97.00%), Postives = 688/700 (98.29%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SSMEEREEEPVEEERIEQ PAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV G+ QS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVAGYHQS 180

Query: 181 SEEEEGIPELEEETVEKNQRVEDVGEKEEENQLKKG-LKVIDTPVEGRELLEALQDVEDY 240
           SEEEEGIPELEEETVEKNQRVED+GEKEEE+QLKK  L+VIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGIPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGI+VSRML ANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLGANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMD+GSHSSTLGRLYAWEKKLYEEVKAG+SMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGNSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420
           LRNQDVKGSNGMTT KTRV VKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG
Sbjct: 361 LRNQDVKGSNGMTTYKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWR CFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRLCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCSRG+TSAVP GLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDR+ILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRQILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANNSKNINYIEGSSQTEENVSR 698
           FSKASQKMYDHLVNYSENAN SKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Cp4.1LG16g03170 vs. NCBI nr
Match: XP_038902520.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1-like [Benincasa hispida] >XP_038902521.1 protein ALTERED PHOSPHATE STARVATION RESPONSE 1-like [Benincasa hispida])

HSP 1 Score: 1169 bits (3024), Expect = 0.0
Identity = 632/728 (86.81%), Postives = 653/728 (89.70%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP CS S  PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTSTE
Sbjct: 61  SSPSPFLITFPPPCSPS--PPSEKVITNPMFLQQMPSDSTHETIATCPSCV-SSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SS+EEREEE VEEERIEQ P Y YMQMPPPMPSPQREF WDFFNPFDTMRTDVVG +R+ 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYLYMQMPPPMPSPQREFGWDFFNPFDTMRTDVVGEYREE 180

Query: 181 S----EEEEGIPELEEETVEK---NQRV------EDVG---------------EKEEENQ 240
                 EEEGIPELEE  VEK   NQRV      EDVG               EKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEEQNQRVVAVVEEEDVGAFKEQRNGVEMVKVGEKEDEGQ 240

Query: 241 LK-KGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKEN 300
           +K KGL VIDTPVEGRELLEALQDVEDYFIRAYDSGI+VSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 MKQKGLNVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTLGR 360
           STKLIQAITWHRS SGK SSCKSLVASSSKGSS WTEF NELFDDYD+MD+GSHSSTLGR
Sbjct: 301 STKLIQAITWHRSASGKPSSCKSLVASSSKGSSAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQD+KG NG+TTDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDIKGENGVTTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF SRG+TS 
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRTST 540

Query: 541 VPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           +P GLHGPPLLSIC DWLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 IPYGLHGPPLLSICHDWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILSFQKTENKF EFNFTETKSELEVENQNEYLTEKKDQLDMFRKKL+LEKEKH
Sbjct: 601 GKELDRRILSFQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLELEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEGSS 698
           NNCIQEAQRITLNGIQ+GFS+VF+SLSEFSKASQKMYDHLVN+SEN + S+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQSGFSAVFESLSEFSKASQKMYDHLVNFSENTDKSENINYIEGSS 720

BLAST of Cp4.1LG16g03170 vs. NCBI nr
Match: XP_011649305.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 [Cucumis sativus] >KGN61947.1 hypothetical protein Csa_006313 [Cucumis sativus])

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 620/728 (85.16%), Postives = 647/728 (88.87%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP       PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS++
Sbjct: 61  SSPSPFLITFPPPS-----PPSEKVITNPMFLQQMPSDSTHETIATCPSCI-SSSSTSSQ 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SS+EEREEE VEEERIEQ P YFYMQMPPPMPSPQREF WDFFNPF++MRTDVVG +R+ 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGIPELEEETVEK---NQRVE---------------------DVGEKEEENQ 240
                 EEEGIPELEE  VEK   NQRV                       VG+KE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVEVIKVGDKEDEGQ 240

Query: 241 LK-KGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKEN 300
            K KGL VIDTPVEGRELLEALQDVEDYFIRAYDSG+++SRMLEANKIQLQSGLEEIKEN
Sbjct: 241 FKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDISRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MD+GSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++TDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQI+ELLKGLTR+WKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF SRG+ SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRASA 540

Query: 541 VPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VP GLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSD QMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDIQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSELEVENQNEYLTEKKDQLD+F+KKLDLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDLFKKKLDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEGSS 698
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENAN S+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENINYIEGSS 720

BLAST of Cp4.1LG16g03170 vs. ExPASy TrEMBL
Match: A0A6J1FTE2 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111448612 PE=4 SV=1)

HSP 1 Score: 1291 bits (3340), Expect = 0.0
Identity = 679/700 (97.00%), Postives = 688/700 (98.29%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE
Sbjct: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SSMEEREEEPVEEERIEQ PAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVV G+ QS
Sbjct: 121 SSMEEREEEPVEEERIEQVPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVAGYHQS 180

Query: 181 SEEEEGIPELEEETVEKNQRVEDVGEKEEENQLKKG-LKVIDTPVEGRELLEALQDVEDY 240
           SEEEEGIPELEEETVEKNQRVED+GEKEEE+QLKK  L+VIDTPVEGRELLEALQDVEDY
Sbjct: 181 SEEEEGIPELEEETVEKNQRVEDIGEKEEESQLKKKELRVIDTPVEGRELLEALQDVEDY 240

Query: 241 FIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300
           FIRAYDSGI+VSRML ANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS
Sbjct: 241 FIRAYDSGIDVSRMLGANKIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASS 300

Query: 301 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 360
           SKGSSPWTEFSNELFDDYDIMD+GSHSSTLGRLYAWEKKLYEEVKAG+SMHKLYEKRCSR
Sbjct: 301 SKGSSPWTEFSNELFDDYDIMDSGSHSSTLGRLYAWEKKLYEEVKAGNSMHKLYEKRCSR 360

Query: 361 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420
           LRNQDVKGSNGMTT KTRV VKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG
Sbjct: 361 LRNQDVKGSNGMTTYKTRVMVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 420

Query: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 480
           LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWR CFSKYV
Sbjct: 421 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRLCFSKYV 480

Query: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPDKP 540
           ESQKAYVEALHGWLTKFVVPEVEFCSRG+TSAVP GLHGPPLLSICRDWLSSMEKLPDKP
Sbjct: 481 ESQKAYVEALHGWLTKFVVPEVEFCSRGRTSAVPYGLHGPPLLSICRDWLSSMEKLPDKP 540

Query: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRRILSFQKTENKFLEFNFTETKS 600
           VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDR+ILSFQKTENKFLEFNFTETKS
Sbjct: 541 VAFALKSFTKDMKALSDKQMEELQQKRRVESLAKELDRQILSFQKTENKFLEFNFTETKS 600

Query: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660
           ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE
Sbjct: 601 ELEVENQNEYLTEKKDQLDMFRKKLDLEKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSE 660

Query: 661 FSKASQKMYDHLVNYSENANNSKNINYIEGSSQTEENVSR 698
           FSKASQKMYDHLVNYSENAN SKNINYIEGSSQTEENVSR
Sbjct: 661 FSKASQKMYDHLVNYSENANKSKNINYIEGSSQTEENVSR 700

BLAST of Cp4.1LG16g03170 vs. ExPASy TrEMBL
Match: A0A0A0LME9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G271390 PE=4 SV=1)

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 620/728 (85.16%), Postives = 647/728 (88.87%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP       PPSEKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS++
Sbjct: 61  SSPSPFLITFPPPS-----PPSEKVITNPMFLQQMPSDSTHETIATCPSCI-SSSSTSSQ 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SS+EEREEE VEEERIEQ P YFYMQMPPPMPSPQREF WDFFNPF++MRTDVVG +R+ 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGIPELEEETVEK---NQRVE---------------------DVGEKEEENQ 240
                 EEEGIPELEE  VEK   NQRV                       VG+KE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVEVIKVGDKEDEGQ 240

Query: 241 LK-KGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKEN 300
            K KGL VIDTPVEGRELLEALQDVEDYFIRAYDSG+++SRMLEANKIQLQSGLEEIKEN
Sbjct: 241 FKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDISRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MD+GSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++TDKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSTDKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQI+ELLKGLTR+WKIMLEVHETQKKIILEVKTYSCHSYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCHSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF SRG+ SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSRGRASA 540

Query: 541 VPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VP GLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSD QMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDIQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSELEVENQNEYLTEKKDQLD+F+KKLDLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSELEVENQNEYLTEKKDQLDLFKKKLDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEGSS 698
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENAN S+NINYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENINYIEGSS 720

BLAST of Cp4.1LG16g03170 vs. ExPASy TrEMBL
Match: A0A5D3BI93 (DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00980 PE=4 SV=1)

HSP 1 Score: 1144 bits (2960), Expect = 0.0
Identity = 619/728 (85.03%), Postives = 646/728 (88.74%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP      P P EKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS+E
Sbjct: 61  SSPSPFLITFPP------PSPPEKVITNPMFLQQMPSDSTHETIATCPSCI-SSSSTSSE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SS+EEREEE VEEERIEQ P YFYMQMPPPMPSPQREF WDFFNPF++MRTDVVG +R+ 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGIPELEEETVEK---NQRVE---------------------DVGEKEEENQ 240
                 EEEGIPELEE  VEK   NQRV                       VGEKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVDVIKVGEKEDEGQ 240

Query: 241 LK-KGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKEN 300
           LK KGL VIDTPVEGRELLEALQDVEDYFIRAYDSG++VSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 LKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MD+GSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++ DKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSADKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQI+ELLKGLTR+WKIMLEVHETQKKIILEVKTYSC+SYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCNSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF S+G+ SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSQGRASA 540

Query: 541 VPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VP GLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSE EVENQNEYLTEKKDQLD+FRKK+DLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSEFEVENQNEYLTEKKDQLDLFRKKVDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEGSS 698
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENAN S+N+NYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENMNYIEGSS 720

BLAST of Cp4.1LG16g03170 vs. ExPASy TrEMBL
Match: A0A1S3C7H0 (uncharacterized protein LOC103497347 OS=Cucumis melo OX=3656 GN=LOC103497347 PE=4 SV=1)

HSP 1 Score: 1144 bits (2960), Expect = 0.0
Identity = 619/728 (85.03%), Postives = 646/728 (88.74%), Query Frame = 0

Query: 1   MGCVASKLEEEE-VVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARH 60
           MGCVASKLEEEE VVSICRERKRH+KLAVERRYALAEAH KYCQALYAVSAAIKLFVARH
Sbjct: 1   MGCVASKLEEEEEVVSICRERKRHLKLAVERRYALAEAHCKYCQALYAVSAAIKLFVARH 60

Query: 61  SSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTE 120
           SSPSPFLITFPP      P P EKVI+NPMFLQQ PSDSTHE I  CPSC  SSSSTS+E
Sbjct: 61  SSPSPFLITFPP------PSPPEKVITNPMFLQQMPSDSTHETIATCPSCI-SSSSTSSE 120

Query: 121 SSMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQS 180
           SS+EEREEE VEEERIEQ P YFYMQMPPPMPSPQREF WDFFNPF++MRTDVVG +R+ 
Sbjct: 121 SSIEEREEESVEEERIEQVPPYFYMQMPPPMPSPQREFGWDFFNPFESMRTDVVGEYRED 180

Query: 181 S----EEEEGIPELEEETVEK---NQRVE---------------------DVGEKEEENQ 240
                 EEEGIPELEE  VEK   NQRV                       VGEKE+E Q
Sbjct: 181 ELRMVREEEGIPELEEAEVEKEDGNQRVVAVAEEENVGAFREQRNGVDVIKVGEKEDEGQ 240

Query: 241 LK-KGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKEN 300
           LK KGL VIDTPVEGRELLEALQDVEDYFIRAYDSG++VSRMLEANKIQLQSGLEEIKEN
Sbjct: 241 LKQKGLTVIDTPVEGRELLEALQDVEDYFIRAYDSGVDVSRMLEANKIQLQSGLEEIKEN 300

Query: 301 STKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTLGR 360
           STKLIQAITWHRSVSGK SSCKSLVASSSKGS  WTEF NELFDDYD+MD+GSHSSTLGR
Sbjct: 301 STKLIQAITWHRSVSGKPSSCKSLVASSSKGSPAWTEFKNELFDDYDVMDSGSHSSTLGR 360

Query: 361 LYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAI 420
           LYAWEKKLYEEVKAGDSM KLYEKRCSRLRNQDVKG NG++ DKTRV VKDLYARILVAI
Sbjct: 361 LYAWEKKLYEEVKAGDSMRKLYEKRCSRLRNQDVKGDNGVSADKTRVAVKDLYARILVAI 420

Query: 421 RSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSYLK 480
           RSAESISTRI  LRDDELQPQI+ELLKGLTR+WKIMLEVHETQKKIILEVKTYSC+SYLK
Sbjct: 421 RSAESISTRIEKLRDDELQPQIIELLKGLTRSWKIMLEVHETQKKIILEVKTYSCNSYLK 480

Query: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSA 540
           FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEF S+G+ SA
Sbjct: 481 FCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFYSQGRASA 540

Query: 541 VPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVESL 600
           VP GLHGPPLLSIC +WLSSMEKLPDKPVAFALKSF KDMKALSDKQMEE  QKRRVESL
Sbjct: 541 VPYGLHGPPLLSICHNWLSSMEKLPDKPVAFALKSFAKDMKALSDKQMEEQHQKRRVESL 600

Query: 601 AKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKEKH 660
            KELDRRILS QKTENKF EFNFTETKSE EVENQNEYLTEKKDQLD+FRKK+DLEKEKH
Sbjct: 601 GKELDRRILSLQKTENKFFEFNFTETKSEFEVENQNEYLTEKKDQLDLFRKKVDLEKEKH 660

Query: 661 NNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEGSS 698
           NNCIQEAQRITLNGIQTGFS+VF+SLSEFSKASQKMYDHLVNYSENAN S+N+NYIEGSS
Sbjct: 661 NNCIQEAQRITLNGIQTGFSTVFESLSEFSKASQKMYDHLVNYSENANKSENMNYIEGSS 720

BLAST of Cp4.1LG16g03170 vs. ExPASy TrEMBL
Match: A0A6J1JI88 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111484590 PE=4 SV=1)

HSP 1 Score: 1135 bits (2936), Expect = 0.0
Identity = 593/610 (97.21%), Postives = 598/610 (98.03%), Query Frame = 0

Query: 89  MFLQQRPSDSTHEAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQFPAYFYMQMPP 148
           MFLQQRPSDSTHEAIPACPSCAC SSSTSTESSMEEREEEPVEEERIEQ PAYFYMQMPP
Sbjct: 1   MFLQQRPSDSTHEAIPACPSCACCSSSTSTESSMEEREEEPVEEERIEQVPAYFYMQMPP 60

Query: 149 PMPSPQREFSWDFFNPFDTMRTDVVGGFRQSSEEEEGIPELEEETVEKNQRVEDVGEKEE 208
           PMPSPQREFSWDFFNPFDTMRTDVV G+RQSSEEEEGIPELEEETVEKNQRVEDVGEKEE
Sbjct: 61  PMPSPQREFSWDFFNPFDTMRTDVVAGYRQSSEEEEGIPELEEETVEKNQRVEDVGEKEE 120

Query: 209 ENQLKKGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIK 268
           ENQLKKGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGI+VSRMLEANKIQLQSGLEEIK
Sbjct: 121 ENQLKKGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIDVSRMLEANKIQLQSGLEEIK 180

Query: 269 ENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDAGSHSSTL 328
           ENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMD+GSHSSTL
Sbjct: 181 ENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDYDIMDSGSHSSTL 240

Query: 329 GRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILV 388
           GRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLR+QDVKGSNGMTTDKTRV VKDLYARILV
Sbjct: 241 GRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRSQDVKGSNGMTTDKTRVMVKDLYARILV 300

Query: 389 AIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSY 448
           AIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSY
Sbjct: 301 AIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHSY 360

Query: 449 LKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQT 508
           LKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPE EFCSRG+T
Sbjct: 361 LKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEDEFCSRGRT 420

Query: 509 SAVPCGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVE 568
           S VP GLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVE
Sbjct: 421 STVPYGLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKRRVE 480

Query: 569 SLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDLEKE 628
           SLAKELDRRILSFQKTENKF EFN TE KSE EVENQNEYLTEKKDQLDMFRKKLDLEKE
Sbjct: 481 SLAKELDRRILSFQKTENKFFEFNSTELKSESEVENQNEYLTEKKDQLDMFRKKLDLEKE 540

Query: 629 KHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINYIEG 688
           KHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENAN SKNINYIEG
Sbjct: 541 KHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANKSKNINYIEG 600

Query: 689 SSQTEENVSR 698
           SSQTEENVSR
Sbjct: 601 SSQTEENVSR 610

BLAST of Cp4.1LG16g03170 vs. TAIR 10
Match: AT4G30130.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 238.0 bits (606), Expect = 2.2e-62
Identity = 216/661 (32.68%), Postives = 310/661 (46.90%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHS 60
           MGC  SK +++E V IC++RKR IK AVE R   A  H  Y Q+L  VS A++ ++    
Sbjct: 1   MGCSHSKFDDDEAVQICKDRKRFIKQAVEHRTGFASGHIAYIQSLRKVSDALREYI-EGD 60

Query: 61  SPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDS--THEAIPACPSCACSSSSTST 120
            P  F++    TC +    P +++ S+  F++  P       EA       +   +S S 
Sbjct: 61  EPHEFML---DTCVT----PVKRMSSSGGFIEISPPSKMVQSEAESKLNVNSYLMASGSR 120

Query: 121 ESSMEEREEEPVEEERIEQFPA--YFYMQMPP----------PMPSPQREFSWDFF-NPF 180
              +EE+     E  ++E + A  +F M M            P PSPQ    WDFF NPF
Sbjct: 121 PVRVEEKPPRSPETFQVETYGADSFFGMNMNMNSPGLGSHNIPPPSPQNS-QWDFFWNPF 180

Query: 181 DTM-----RTDVVGGFRQSS------EEEEGIPELEEETVEK------------------ 240
             +       D   G            EEEGIP+LEE+   K                  
Sbjct: 181 SALDQYGYSYDNQNGMDDDMRRLRRVREEEGIPDLEEDEYVKFEDHHNMKATEDFNGGKM 240

Query: 241 --NQRVEDVGEK--------EEEN-------QLKKGLKVIDTPVEGR------------- 300
               +VE V E+        E E+       Q ++ L+V      G              
Sbjct: 241 YQEDKVEHVNEEFTDSGCKIENESDKNCNGTQERRSLEVSRGGTTGHVVGVTSDDGKGET 300

Query: 301 ------------ELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKL 360
                        + E ++D+ED F     +G EVS +LEA+++Q  S  E    ++  +
Sbjct: 301 PGFTVYLNRRPTSMAEVIKDLEDQFAIICTAGKEVSGLLEASRVQYTSSNE---LSAMTM 360

Query: 361 IQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEF-SNELFDDYDIMDAGSHSSTLGRLYA 420
           +  +   RS     SS  S    SS G S  +EF S+  F +   M +GSH STL RLYA
Sbjct: 361 LNPVALFRSGGSSRSSSSSRFLISSSGGSRASEFESSSEFSEESCMLSGSHQSTLDRLYA 420

Query: 421 WEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSA 480
           WEKKLY+EVK+GD +   YEK+C  LRNQDVKG++    DKTR T++DL+ +I V+I S 
Sbjct: 421 WEKKLYDEVKSGDRIRIAYEKKCLVLRNQDVKGADSSAVDKTRATIRDLHTQIKVSIHSI 480

Query: 481 ESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVK----TYSCHSYL 540
           ESIS RI  LRD EL PQ++EL++GL + WK+M E H+ QK+ + E K    T   + + 
Sbjct: 481 ESISERIETLRDQELLPQLLELVQGLAQMWKVMAECHQIQKRTLDEAKLLLATTPSNRHK 540

Query: 541 KFCN------ESHRLA--TLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVE 561
           K          S RLA   L L  +L+NWR+CF  ++ SQ++Y+ +L GWL +       
Sbjct: 541 KQQQTSLPEINSQRLARSALHLVVQLRNWRACFQAWITSQRSYILSLTGWLLR------- 600

BLAST of Cp4.1LG16g03170 vs. TAIR 10
Match: AT4G39790.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 234.2 bits (596), Expect = 3.2e-61
Identity = 205/656 (31.25%), Postives = 318/656 (48.48%), Query Frame = 0

Query: 1   MGCVASKL---EEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLF-- 60
           MGC  SK    ++ E + +C+ERKR +K A++ R ALA AH  Y ++L  + A ++ +  
Sbjct: 9   MGCSNSKASMNKKNEPLHLCKERKRFVKQAMDSRCALAAAHVSYIRSLRNIGACLRQYAE 68

Query: 61  --VARHSSPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSS 120
              A  SSPS    T P    S +    +  + +P+     P+ +        P    S 
Sbjct: 69  AETAHESSPS-LTATEPEKSPSHNSSYPDDSVDSPLSHNSNPNPNPK------PLFNLSY 128

Query: 121 SSTSTESSMEEREEEPV---EEERIEQFPAYFYMQMPPPMPSPQR--EFSWDFF---NPF 180
             T T SS       P+   +++     PA+     PPP P P+R    SWD+F   + F
Sbjct: 129 MKTETASSTVTFTINPLSDGDDDLEVTMPAF----SPPPPPRPRRPETSSWDYFDTCDDF 188

Query: 181 DTMR---------------TDVVGGFRQSSE------EEEGIPELEEETVEKNQRVEDVG 240
           D+ R                 V+G  + +S+        E + +   +T ++ Q  ED  
Sbjct: 189 DSFRFVGLSEQTEIDSECDAAVIGLEKITSQGNVAKSGSETLQDSSFKTKQRKQSCEDND 248

Query: 241 EKEEENQLKKGLKVIDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGL 300
           E+E+ ++              ++ + +++D+E  F RA +SG EVSRMLE NKI++    
Sbjct: 249 EREDPSEF--------ITHRAKDFVSSMKDIEHKFFRASESGREVSRMLEVNKIRVGFAD 308

Query: 301 EEIKENSTKLIQA--------------------------ITWHRSVSGKTSSCKSLVASS 360
              K NS   + A                          I W R+ S ++S+ ++ +  +
Sbjct: 309 MTGKGNSIAFLAALKRACCRGKSYSPVSQEPLSHQVTKVIVWKRTSSSRSSTSRNPLIQT 368

Query: 361 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 420
           SK      E  ++  +++  M +GSHSS+L RLYAWE+KLY+EVKA + + K Y+++C +
Sbjct: 369 SKEDHD-DESGSDFIEEF-CMISGSHSSSLDRLYAWERKLYDEVKASEMIRKEYDRKCEQ 428

Query: 421 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 480
           LRNQ  K  +  + DKTR   KDL++RI VAI+S ESIS RI  +RDDEL PQ++E L+G
Sbjct: 429 LRNQFAKDHSAKSMDKTRAAAKDLHSRIRVAIQSVESISKRIERIRDDELHPQLLEFLQG 488

Query: 481 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNES--HRLATLQLGAELQNWRSCFSK 540
           L R WK MLE H TQ   I     Y C    K  +ES   R    +L  E + +   F  
Sbjct: 489 LIRMWKAMLECHHTQ--YITISLAYHCRHSSKTAHESVLKRRILAELLEETECFGLSFVD 548

Query: 541 YVESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPD 591
            V S  +YVEAL+GWL   V+   E  +R +    P  +  PP+  +CRDW + ++ LP 
Sbjct: 549 LVHSMASYVEALNGWLHNCVLLPQERSTRNRRPWSPRRVLAPPIFVLCRDWSAGIKTLPS 608

BLAST of Cp4.1LG16g03170 vs. TAIR 10
Match: AT3G51290.2 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 231.1 bits (588), Expect = 2.7e-60
Identity = 196/721 (27.18%), Postives = 345/721 (47.85%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHS 60
           MGC  S+++ +E+VS C+ RKR++K  V+ R  L+ +H+ Y ++L AV +++  F    S
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHF---SS 60

Query: 61  SPSPFLITFPPTCSSSSPPPSEKVISNPMFLQQRPSDSTHEAIPACPSCACSSSSTSTES 120
             +P  +   P   S  PPP             RP        P  P    ++ +T+T S
Sbjct: 61  KETPLHLHHNPPSPSPPPPP-----------PPRPPPP-----PLSPGSETTTWTTTTTS 120

Query: 121 SMEEREEEPVEEERIEQFPAYFYMQMPPPMPSPQREFSWDFFNPFDTMRTDVVGGFRQSS 180
           S+                        PPP P P    +WDF++PF       +     SS
Sbjct: 121 SV-------------------LPPPPPPPPPPPPPSSTWDFWDPF-------IPPPPSSS 180

Query: 181 EEEEGIPELEEETVEKNQRVEDVG-----------------------EKEEENQLKKGLK 240
           EE     E EEET    +     G                        K+       G +
Sbjct: 181 EE-----EWEEETTTATRTATGTGSDAAVTTAPTTATPQASSVVSGFSKDTMTTTTTGSE 240

Query: 241 V-IDTPVEGRELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQSGLEEIKENSTKLIQ 300
           + +     G++L+E +++V++YF++A DSG  +S +LE     + + + +   +S     
Sbjct: 241 LAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSSLLE-----ISTSITDFSGHSK---- 300

Query: 301 AITWHRSVSGKTSSCKSLVASSSKGSSPWTE-FSNELFDDYDIMD--------AGSHSST 360
                   SGK  S  +   + +  +S WT  F+     +Y             GSHSST
Sbjct: 301 --------SGKMYSSSNYECNLNP-TSFWTRGFAPSKLSEYRNAGGVIGGNCIVGSHSST 360

Query: 361 LGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTRVTVKDLYARIL 420
           + RLYAWEKKLY+EVK  +S+   +EK+  ++R  ++K +  + T+K +  V+ L +++ 
Sbjct: 361 VDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQLS 420

Query: 421 VAIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKIILEVKTYSCHS 480
           V+ ++ +S S  I+ LR+ EL PQ+VEL+KGL   W+ M E H+ Q  I+ ++K  +   
Sbjct: 421 VSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQVQTHIVQQLKYLNTIP 480

Query: 481 YLKFCNESHRLATLQLGAELQNWRSCFSKYVESQKAYVEALHGWLTKFVVPEVEFCSRGQ 540
             +  +E HR +TLQL  E+Q W   F   V++Q+ Y+++L GWL           S  Q
Sbjct: 481 STEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWLR---------LSLFQ 540

Query: 541 TSAVPC--GLHGPPLLSICRDWLSSMEKLPDKPVAFALKSFTKDMKALSDKQMEELQQKR 600
            S  P     +   + S C +W  +++++PDK  +  +KSF   +  +  +Q +E +QK+
Sbjct: 541 FSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQADEHKQKK 600

Query: 601 RVESLAKELDRRILSFQKTENKFLEFNFTETKSELEVENQNEYLTEKKDQLDMFRKKLDL 660
           R ES+ K+ +++  S +  E+K+  ++  E++ +  V        EK+ +++M + K + 
Sbjct: 601 RTESMLKDFEKKSASLRALESKYSPYSVPESRKKNPV-------IEKRVKVEMLKGKAEE 637

Query: 661 EKEKHNNCIQEAQRITLNGIQTGFSSVFDSLSEFSKASQKMYDHLVNYSENANNSKNINY 687
           EK KH   +   + +TLN +Q GF  VF ++  FS    + ++ + N +++    +  +Y
Sbjct: 661 EKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQEEDY 637

BLAST of Cp4.1LG16g03170 vs. TAIR 10
Match: AT2G19090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 226.5 bits (576), Expect = 6.7e-59
Identity = 215/740 (29.05%), Postives = 320/740 (43.24%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIKLFVARHS 60
           MGC  SKL++EE V IC++RKR IK A+E R   A  H  Y  +L  VS A+  F+ +  
Sbjct: 1   MGCSHSKLDDEEAVQICKDRKRFIKQAIEHRTKFASGHIAYIHSLRKVSDALHDFILQGD 60

Query: 61  SPSPFLITFPPTCSSSSPPPSEKV---------ISNPMFLQQRPSDSTHEAIPACPSCAC 120
           + + F+   P  C  S   P +++          ++  F+   PS    + I   P    
Sbjct: 61  NNNEFV---PTLCQDSFVTPVKRMPQRRRRSSRSNSGEFISISPSSIPPKMIQGRPRSNV 120

Query: 121 SSSSTSTESSMEER-EEEPVEEERIEQF------------PAYFYMQMPP---------- 180
            ++      S   R E+   E  R+E F              +F M M            
Sbjct: 121 KANYLMANRSRPVRVEQRSPETFRVETFSPPSSSNQYGESDGFFGMNMNMNTSASTSSSF 180

Query: 181 ----------------PMPSPQREFSWDFF-NPFDTMR------------------TDVV 240
                           P PSPQ    WDFF NPF ++                    D +
Sbjct: 181 WNPLSSPEQRLSSHNIPPPSPQNS-QWDFFWNPFSSLDYYGYNSYDRGSVDSRSGIDDEI 240

Query: 241 GGFRQSSEEEEGIPELEEE----------------------TVEKNQ------------R 300
            G R+   EEEGIP+LEE+                        E+N+            +
Sbjct: 241 RGLRR-VREEEGIPDLEEDDEPHKPEPVVNVRFQNHNPNPKATEENRGKVDKSCCNEEVK 300

Query: 301 VEDVGEKEEENQLKKGLKVID----TPVEGRE---------------------------- 360
           VEDV E E+E+  +   +  D    T  EG E                            
Sbjct: 301 VEDVDEDEDEDGDEDDDEFTDSGCETENEGDEKCVAPPTQEQRKAEVCRGGEATGNVVGV 360

Query: 361 -------------------------------LLEALQDVEDYFIRAYDSGIEVSRMLEAN 420
                                          + E ++D+ED F    D+  EVS +LEA 
Sbjct: 361 GKVQEMKNVVGVRDDAKTTGFTVYVNRRPTSMAEVIKDLEDQFTTICDAAKEVSGLLEAG 420

Query: 421 KIQLQSGLEEIKENSTKLIQAITWHRSVSGKTSSCKSLVASSSKGSSPWTEFSNELFDDY 480
           + Q  S   +   ++ K++  +   RS S ++SS + L+ SS       +E  +++ D+ 
Sbjct: 421 RAQYTSSFND--HSARKMLNPVALFRSGSSRSSSSRFLITSSGGSRESGSESRSDVSDE- 480

Query: 481 DIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSRLRNQDVKGSNGMTTDKTR 540
             M +GSH +TL RL+AWEKKLY+EV++G+ + + YEK+C +LRNQDVKG + +  DKTR
Sbjct: 481 SCMISGSHQTTLDRLFAWEKKLYDEVRSGERVRRAYEKKCMQLRNQDVKGDDPLAVDKTR 540

Query: 541 VTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKGLTRTWKIMLEVHETQKKI 561
            T++DL  +I V+I S ESIS RI  LRD EL PQ++EL++GLTR WK+M E H+ QK+ 
Sbjct: 541 ATIRDLDTQIKVSIHSIESISKRIETLRDQELLPQLLELVEGLTRMWKVMAESHQIQKRT 600

BLAST of Cp4.1LG16g03170 vs. TAIR 10
Match: AT2G27090.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 221.5 bits (563), Expect = 2.2e-57
Identity = 206/730 (28.22%), Postives = 331/730 (45.34%), Query Frame = 0

Query: 1   MGCVASKLEEEEVVSICRERKRHIKLAVERRYALAEAHSKYCQALYAVSAAIK------- 60
           MG   S+++E++ + +CRERK+ ++ A++ R  LA AH  Y Q+L +   A++       
Sbjct: 1   MGASTSRIDEDKALQLCRERKKFVQQALDGRCLLAAAHVSYVQSLKSTGTALRKFSETEV 60

Query: 61  -----LFVARHSSPSPFLITF----------PPTCSSS-----SPPPSEKVIS----NPM 120
                L+ +  ++P   L             PP  S S     SPPPS    S    N M
Sbjct: 61  PVESSLYTSTSATPEQPLALIEKSVSHLSYSPPPASHSHHDTYSPPPSPPSTSPFQVNHM 120

Query: 121 FLQQRPSDSTHEAIPACPSCACSSSSTSTESSMEEREEEPVEEERIEQFPAYFYMQMPPP 180
             +   S    E  P       +SSS     S+E+ E  P EE             MPP 
Sbjct: 121 KFKGFSSKKVEEKPPVSIIATVTSSSIPPSRSIEKMESTPFEES----------SSMPPE 180

Query: 181 MPSPQREFSWDFF---NPFDTMRTDV---VGGFRQSSEEEEGIPELEEE----------- 240
            P       WD+F   +P D   +      G   +S +EE+G PE E++           
Sbjct: 181 AP-------WDYFGLSHPIDNQFSSSHVGNGHVSRSVKEEDGTPEEEDDGEDFSFQEREE 240

Query: 241 --------------------------------TVEKNQRVEDVGEKEEENQLKK------ 300
                                           T+ + + VE      E+++  +      
Sbjct: 241 SRDSDDDEFDEPTSDTLVRSFENFNRVRRDHSTLPQREGVESDSSDAEKSKTPELSPPVT 300

Query: 301 ---GLKVIDTPVEG---------RELLEALQDVEDYFIRAYDSGIEVSRMLEANKIQLQ- 360
                 V  TP +G         R+ L +++++E  F++A ++G EV RMLEANK+  + 
Sbjct: 301 PLVATPVNKTPNKGDHTENKLPPRDFLSSMKEIELLFVKASETGKEVPRMLEANKLHFRP 360

Query: 361 --------SGLEEI---------------KENSTKLIQAITWHRSVSGKTSSCKSLVASS 420
                   SG   +               +E +   ++ +TWHR+ S ++SS ++ +   
Sbjct: 361 IVPSKESGSGASSLFKTCLSCGEDPKDVPEEPAQNSVKYLTWHRTESSRSSSSRNPLGGM 420

Query: 421 SKGSSPWTEFSNELFDDYDIMDAGSHSSTLGRLYAWEKKLYEEVKAGDSMHKLYEKRCSR 480
           +  S    E ++ LF++   M AGSH+STL RLYAWE+KLY+EVK   ++ + Y+++C  
Sbjct: 421 N--SDDVEELNSNLFENI-CMIAGSHASTLDRLYAWERKLYDEVKGSQTVRREYDEKCRI 480

Query: 481 LRNQDVKGSNGMTTDKTRVTVKDLYARILVAIRSAESISTRIVNLRDDELQPQIVELLKG 540
           LR  + +G      DKTR  VKDL++RI VAI   +SIS RI  LRD+ELQPQ+ EL++G
Sbjct: 481 LRELESEGKGSQRIDKTRAVVKDLHSRIRVAIHRIDSISRRIEELRDNELQPQLEELIEG 540

Query: 541 LTRTWKIMLEVHETQKKIILEVKTYSCHSYLKFCNESHRLATLQLGAELQNWRSCFSKYV 600
           L+R W++MLE H+ Q ++I +      +  L   +E HR  T  L  EL    S F+K++
Sbjct: 541 LSRMWEVMLECHKVQFQLI-KACYRGGNIKLNMQSELHRQVTSHLEDELCALASSFTKWI 600

Query: 601 ESQKAYVEALHGWLTKFVVPEVEFCSRGQTSAVPCGLHGPPLLSICRDWLSSMEKLPDKP 609
             QK+Y++A++ WL K V        + +        +GPP+ + C  WL  +E LP K 
Sbjct: 601 TGQKSYIQAINEWLVKCVALPQRSKRKRRAPQPSLRNYGPPIYATCGIWLEKLEVLPTKE 660