Cp4.1LG16g02210 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g02210
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING/U-box superfamily protein
LocationCp4.1LG16: 4384069 .. 4389236 (+)
RNA-Seq ExpressionCp4.1LG16g02210
SyntenyCp4.1LG16g02210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACTTCATCGGAGCGACTACCATTTAGTCGTTGTCCTTTCACTCTCTTCGCCGGCTTTTCCAACGATCTCCCCGGAGGGAAACGTTCTCCGGCGTTCCCCGATTCTCCGGATCGGATTTATTCATCGAATCTCTCTCGTTATCGATTGATATTTTTTCTTACTTGTATTACCAACTGAGCTTTGCTTTTTTATTTTTTATTTTTCCATCTGACTTCAATTTGTCTTCTCTTTCCCCAATTCGATTTCGCTATTTCTTTCGCCAATTTTTCAATGGAGGACTGCATCAGCAGCGATGAAGACTACTATGACTCCGATCGTGAGTCTCTTCAGGGTCTCGAGAACGAGGACTCTGAAATTCAGTGGGTTTCTAAAGGTCCCGCGACTAAGGTATTCTATTTTTTTTTTGGTACGAGGAGGGATTCTAATTGCGATTGATTACTTGGTTTGCTGATTGAAAGTTGTAACTCTTGTTGTTGGATTCAATTTTAGTGAGTTTCTTTTGTGGTGATGGCAGGGAATGAATTGATTGGGTTGAAATATAACGCTCCATGTTTTGTTTTTTGGACTCTTACTGTTTTTGTTGTTATTATTACTCTTTCCTGAAAAAAAGAGGTTTTTCTCCAAGTTTGTGATGATGGGGCGGCGGAATTTGGTGCATAATGCTTGGAATTTCTTTCCTGTTGAGATTCATGTGAATTTTCTAATCAATCATGCTTATGAAAACCCTTTTTGTTGTTTTCAGGTTATTACAAAGGAATCTCTCCTTGCTGCACAGGTTTAGCACCGGTTCTTCCCATCATATAAAAGACAATTTATCTCATTCTATTCTTTCATTGGATTCATGGGTTCTTTTTCGTTATTTGAATCTGATTTTAGGGCCGTTTTGTTTTCGTGTAGAAGGAGGACATGCGTAGAGTAATGGAGTTACTATCATTGAGGGAGCACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCACTTGTTTGCTACTGCTGGTGTTACTATGATTGAGAATCGAAGTAATTGTTCCTCTGAGACTTCCTCCGTGGTGATGTGTGATATCTGCATGGAGGAAGTACATAGCAATGATGCAACAAGAGTGGATTGTGGTCATTGCTTTTGCAATAACTGTAAGCCTTTTTTTCCCTCTAAAATTAGATTTTGAATGGCCTTGTGAACTAATATTGTAAAGATTGTCTGTGATGTTAGTTGTAAGCAATTGTTATGGAATCAAATTGTGCTAAGTTCGTATAAATGACTAATAATTGGCTGAGCTTTATTTCTTGCTTCAGGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAGAGTCGTAGAATCAGATGCATGGCCCACAAATGTAATGCAATTTGTGATGAAGCTGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAATAAATTTGATCGTTTCCTTCTTGAATCATACATCGAGGATAACAAAAGAGTCAAGTGGTGTCCGAGCGCTCCTCATTGCGGCAATGCTATACGTGTGGAGGATGATGAGTTCTGTGAAGTAGAATGTTCTTGTGGTCTTCAGTTTTGTTTCAGTTGCTTATCAGATGCTCACTCGCCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTGGAGAAGAATGGAGGCTGTAATCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGGTAAGATTATACTATTTTTTTAATCATTTTCTTTGTTATAGTTTTTTCAGTAAATGGGTGAAATGGCACATTCTGTTCAAAACTACTTAGATATGAGACCTGAAATCGATCCCTTTCATATATTGGCGAGATTTTGAGGTAGATAGCTCATTTTCATATTTTGCTGAAACTGAGACCGACACTGCAAGTATCGGCCAGTGTGGCACTCGTCAGATACTTACTGTTCATAGTTAGGTTCGATATAAGACAAATGGTCATACATCTAAATTCTTCTCTTGAAAAGCATACATGATTTTAGCGGCCTTGAAGAAGTAACTTTCTGCATTTCATTTGATGTGAAAATGTTCTGAAGAATCTAGTCATTTGATGCCCTTTCTGTCTCTTTCTATGAACAAATTTTTTTTTCTTTGCTATTGTCTTTTTAATATATGCCATTGATTCATATAATGGTGCGCACCACTTTTTGAGGTCTCAAACAATTTGTGAGTGGTCAATAATCTTCGTGATTTTTTTAATAAGTTTTTAGACCTTTTGGGAATTTGCTAATTTGTTATCAATAGAATTTAAATGATCTCACATCATGGTTTGTTTTTTCTTTATAGATGATAGATTAGTTAGATGTATATTGGAAATTTAGAACAAGTTCTAAGAAGGATTGCAGTTAATTAGATAGTAATCTTAGATAGTACTACCTAATCCATCTCCATTACTTTGATTGAAACTTAGAATAGGAAAATTTTCAATTGAATATTATGGTTTGAACTACCATAATTCACTCTAATATTTTTCTACATTCACTTGAACAAGTTCAATATAACGTATTTAATCATTTTAACCTTATGCTGGTTATAGATTCTGTAAAAATACGAATCTCCATGATCTAAGGAAATAAGTTTAGTTAGGTGCGAAGTATGATGTGTGAAAGATGGTTATTTTTTCGTTTAACTCACCAAATAATTACTTAAACCTTGCTTCAATTTGAATATAAATTCATATAAGTCGAGCATTTAGTGCTTAAGACATAAGTTTATGATAGTATAACTGTTTCTTTTTCCAATTGTTATTCTTTACCATAAAATTCATTTGAATAATATTTTCATAACACTTCAATTTTAGCATATGGACGGCAGTCGTTAATTTCATCTCTTAACCAGCTGCTTGTTTCTATTTTAGCTATGTGTTCTTCTCACCTTGTTTTACTTTTATTAAGTGGGTCTAAGTTTCTAATTCCCATCCTTCTGGTACAGTTGGCTGTGTGGTGGAGCCACTGGTCGAGAGCACACTTGGTCGAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTACCGTTATATGCACTATCATAATCGATACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCCATACAAGAGAAGATCTCGATTTCTGAAGAGAAGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTGTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCTTACCCATTTGCGTTCTATATGTTTGGGGATGAGCTATTCAAAGACGAGATGACTCAGGAGGAAAGGGAGATAAGGCAGCATCTATTTGAGGACCAACAGCAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTAGAGGAACCGTTTGATCAATACACGAAAGACAGGGTAATGGATATAAGGATGCAAGTCATCAATCTGTCGGTTATCACCGATACCCTCTGCAAGAAGATGTAAGTATACTGAGCTATCTGTTCTTCTAAAAACGTGTAAAGCTCTGGGAAGTCATGTCTTATTCTAACGCAGGTACGACTGTATTGAGAACGATCTTCTGGGTTCACTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATTGAGAAGGCGCTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGAACAAGTGGTAAAGTGGAGAGTACTTTTCTACACTTTTTTATTCATCCCTGTTCGGTTCACAAATCGTGATTTCGATTCGTCTGTCTCAGTTCACAAATTTTGATCTCAATGCGTCTGTCTCTTAACAGTGTCCCTTATTTTTGAAAATATACATTTATCATCTACATCTCTAAATCAAGATCATTGGGCATTGCTTGGTCTTTCTTTAGTCAGATAAATCTTTATTTTGGGCCTATTTGATGTGGAGTTCTTACAATTCTTTTCAGTCAATTTCTGAGACAATATATAAAGGAAACTACCTGGCAATACTGCTTTGTATAGATATTGGGTATAAAAGTTTTACTGATGGTATAAATGCAGGAGGATTGTCAGAGCGGAATAGACTGAGTAGCTTGAATTCCGATGAGAACGGATGCTCGTCTAGGAAGCGTGCCAGAGCCGAAGTCGTTACTGGCAACTGCTTCGATCTAAACTTACCTGCTGAGGTTGTTGATAGAAACTGATCTCTGGAAGTTGCGAGTTTATAACGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCGTTAGCACGGGGACGGGCAATCGAGGATTGGCAATCCTAGGTACTTTTAACTCTTTACTCTTTGTTTATCATGTTTGTCTCAGGTTGAATATATTGTCTTCGGCTGAGCTATATTTTGAAAGAGAATTATCTTTAAGTTTCACGTTCTTTACTTTTGAAATCCGCAAATTGTACATAGCAGTGATATCGGGATTTGATGATGGTATTTGGAATGTAAATGCTTAATTCTCAGCTTGAGACAGACAAGAGGGTATATGCATTTTGAGCTCTAGTCTAATATTCTCTCTTGCTGGAAGTAAAATTTCTATGCTGTGTTTTATTCGATGTTTCCTGATGTTCATGTTTGTGTGCTATGAACATTTCCATGTCTGGTGATCTGTTTTTGGTCATATCCTGATCTTTGCCTTTTACAGGAAGAAATAATGATAAAAGCTGGTTATAGCTGTTGGAATCTTTGGCATTTCATGATTTTGTTTTTAGTTAAACACGGGAGTAAAGTCGAACCTTCAACTTCTTCTTGATTGAAGATATGTGTTTTAACTAGTTGAGTTATGCTAATGTTGACGTTTTTAGTGGTTTATAATTGGAAGTGTCACTTTCTTGGTGGGTTATAGCTGTTGGAATGTTCCTCATTTCACGATTTTATTTTTAGTTAAACACGAGTGAAGTCGAACCTTCAACTTCTTGGTTGAAGATATGTATTTTAACTAGTTGAGTTATGTTTATGTTGACGTTTTTGAGTGGTTTATAATT

mRNA sequence

TACTTCATCGGAGCGACTACCATTTAGTCGTTGTCCTTTCACTCTCTTCGCCGGCTTTTCCAACGATCTCCCCGGAGGGAAACGTTCTCCGGCGTTCCCCGATTCTCCGGATCGGATTTATTCATCGAATCTCTCTCGTTATCGATTGATATTTTTTCTTACTTGTATTACCAACTGAGCTTTGCTTTTTTATTTTTTATTTTTCCATCTGACTTCAATTTGTCTTCTCTTTCCCCAATTCGATTTCGCTATTTCTTTCGCCAATTTTTCAATGGAGGACTGCATCAGCAGCGATGAAGACTACTATGACTCCGATCGTGAGTCTCTTCAGGGTCTCGAGAACGAGGACTCTGAAATTCAGTGGGTTTCTAAAGGTCCCGCGACTAAGGTTATTACAAAGGAATCTCTCCTTGCTGCACAGAAGGAGGACATGCGTAGAGTAATGGAGTTACTATCATTGAGGGAGCACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCACTTGTTTGCTACTGCTGGTGTTACTATGATTGAGAATCGAAGTAATTGTTCCTCTGAGACTTCCTCCGTGGTGATGTGTGATATCTGCATGGAGGAAGTACATAGCAATGATGCAACAAGAGTGGATTGTGGTCATTGCTTTTGCAATAACTGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAGAGTCGTAGAATCAGATGCATGGCCCACAAATGTAATGCAATTTGTGATGAAGCTGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAATAAATTTGATCGTTTCCTTCTTGAATCATACATCGAGGATAACAAAAGAGTCAAGTGGTGTCCGAGCGCTCCTCATTGCGGCAATGCTATACGTGTGGAGGATGATGAGTTCTGTGAAGTAGAATGTTCTTGTGGTCTTCAGTTTTGTTTCAGTTGCTTATCAGATGCTCACTCGCCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTGGAGAAGAATGGAGGCTGTAATCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGTTGGCTGTGTGGTGGAGCCACTGGTCGAGAGCACACTTGGTCGAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTACCGTTATATGCACTATCATAATCGATACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCCATACAAGAGAAGATCTCGATTTCTGAAGAGAAGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTGTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCTTACCCATTTGCGTTCTATATGTTTGGGGATGAGCTATTCAAAGACGAGATGACTCAGGAGGAAAGGGAGATAAGGCAGCATCTATTTGAGGACCAACAGCAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTAGAGGAACCGTTTGATCAATACACGAAAGACAGGGTAATGGATATAAGGATGCAAGTCATCAATCTGTCGGTTATCACCGATACCCTCTGCAAGAAGATGTACGACTGTATTGAGAACGATCTTCTGGGTTCACTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATTGAGAAGGCGCTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGAACAAGTGGAGGATTGTCAGAGCGGAATAGACTGAGTAGCTTGAATTCCGATGAGAACGGATGCTCGTCTAGGAAGCGTGCCAGAGCCGAAGTCGTTACTGGCAACTGCTTCGATCTAAACTTACCTGCTGAGGTTGTTGATAGAAACTGATCTCTGGAAGTTGCGAGTTTATAACGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCGTTAGCACGGGGACGGGCAATCGAGGATTGGCAATCCTAGGAAGAAATAATGATAAAAGCTGGTTATAGCTGTTGGAATCTTTGGCATTTCATGATTTTGTTTTTAGTTAAACACGGGAGTAAAGTCGAACCTTCAACTTCTTCTTGATTGAAGATATGTGTTTTAACTAGTTGAGTTATGCTAATGTTGACGTTTTTAGTGGTTTATAATTGGAAGTGTCACTTTCTTGGTGGGTTATAGCTGTTGGAATGTTCCTCATTTCACGATTTTATTTTTAGTTAAACACGAGTGAAGTCGAACCTTCAACTTCTTGGTTGAAGATATGTATTTTAACTAGTTGAGTTATGTTTATGTTGACGTTTTTGAGTGGTTTATAATT

Coding sequence (CDS)

ATGGAGGACTGCATCAGCAGCGATGAAGACTACTATGACTCCGATCGTGAGTCTCTTCAGGGTCTCGAGAACGAGGACTCTGAAATTCAGTGGGTTTCTAAAGGTCCCGCGACTAAGGTTATTACAAAGGAATCTCTCCTTGCTGCACAGAAGGAGGACATGCGTAGAGTAATGGAGTTACTATCATTGAGGGAGCACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCACTTGTTTGCTACTGCTGGTGTTACTATGATTGAGAATCGAAGTAATTGTTCCTCTGAGACTTCCTCCGTGGTGATGTGTGATATCTGCATGGAGGAAGTACATAGCAATGATGCAACAAGAGTGGATTGTGGTCATTGCTTTTGCAATAACTGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAGAGTCGTAGAATCAGATGCATGGCCCACAAATGTAATGCAATTTGTGATGAAGCTGTTGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCCAATAAATTTGATCGTTTCCTTCTTGAATCATACATCGAGGATAACAAAAGAGTCAAGTGGTGTCCGAGCGCTCCTCATTGCGGCAATGCTATACGTGTGGAGGATGATGAGTTCTGTGAAGTAGAATGTTCTTGTGGTCTTCAGTTTTGTTTCAGTTGCTTATCAGATGCTCACTCGCCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGTCCAAAGTGCCACAAACCTGTGGAGAAGAATGGAGGCTGTAATCTCGTCAGCTGCATCTGTGGTCAAGCATTTTGTTGGCTGTGTGGTGGAGCCACTGGTCGAGAGCACACTTGGTCGAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTACCGTTATATGCACTATCATAATCGATACAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCCATACAAGAGAAGATCTCGATTTCTGAAGAGAAGGAATCCATGCTCAGAGATTTTAGTTGGGTAAACAATGGACTGTGTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCTTACCCATTTGCGTTCTATATGTTTGGGGATGAGCTATTCAAAGACGAGATGACTCAGGAGGAAAGGGAGATAAGGCAGCATCTATTTGAGGACCAACAGCAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTAGAGGAACCGTTTGATCAATACACGAAAGACAGGGTAATGGATATAAGGATGCAAGTCATCAATCTGTCGGTTATCACCGATACCCTCTGCAAGAAGATGTACGACTGTATTGAGAACGATCTTCTGGGTTCACTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATTGAGAAGGCGCTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTACAGACAAATATCTGCCATCAGACTGTGGAACAAGTGGAGGATTGTCAGAGCGGAATAGACTGAGTAGCTTGAATTCCGATGAGAACGGATGCTCGTCTAGGAAGCGTGCCAGAGCCGAAGTCGTTACTGGCAACTGCTTCGATCTAAACTTACCTGCTGAGGTTGTTGATAGAAACTGA

Protein sequence

MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCGTSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Homology
BLAST of Cp4.1LG16g02210 vs. ExPASy Swiss-Prot
Match: Q84RR2 (Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI2 PE=2 SV=1)

HSP 1 Score: 851.7 bits (2199), Expect = 5.1e-246
Identity = 424/597 (71.02%), Postives = 488/597 (81.74%), Query Frame = 0

Query: 1   MEDCISSDED--YYDSDRESLQGLENEDS-EIQWVSKGPATKVITKESLLAAQKEDMRRV 60
           M+D +S +E+  YY SD+ESL G++N++S  I   S+    KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 61  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRS--NCSSETSS 120
           MELLS++EHHARTLLIHYRWDVEKLFAVLVEKGK  LF+ AGVT++EN+S  +  S +SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 121 VVMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 180
           ++ CDIC+E+V     TR+DCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 181 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCG 240
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPS PHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 241 LQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300
           LQFCFSC S AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 301 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 360
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 361 HTDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMF 420
           H DS KLE+KL  +I +K+SISE++E  L+DFSW  NGL RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 421 GDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVIN 480
           GDELFKDEM+ EEREI+Q+LFEDQQQQLEANVEKLSKFLEEPFDQ+  D+VM IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 481 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYL 540
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +  NSK        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 541 PSDCGTSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGN----CFDLNLPAEVVDR 589
            SDCG +  L +   L S  S++  CSS KRAR +    N      DLNLPAE ++R
Sbjct: 541 -SDCGWTSRLDQ--ALESGKSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of Cp4.1LG16g02210 vs. ExPASy Swiss-Prot
Match: Q949V6 (Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI1 PE=2 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 1.2e-244
Identity = 415/599 (69.28%), Postives = 497/599 (82.97%), Query Frame = 0

Query: 1   MEDCISSDED--YYDSDRESLQGLENEDSEIQWV-SKGPATKVITKESLLAAQKEDMRRV 60
           M+D  S++E+  YY SD++SL G++NE+SE+Q + SK   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENR-SNCSSETSSV 120
           MELLS++EHHARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  N S   SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++  +  TR+DCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPS PHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL  AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE++E  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINL 480
           +E+FKDEMT EEREI+++LFEDQQQQLE+NVEKLS+FLEEPFD+++ D+VM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERNRLSSLNSDENG--CSSRKRARAEVVTGN----CFDLNLPAEVVDRN 590
            D GTSG  S   + S   S E+    SS+KR + E    N      DLNLPA+ VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 597

BLAST of Cp4.1LG16g02210 vs. ExPASy Swiss-Prot
Match: Q9LVX0 (Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI3 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 8.5e-185
Identity = 325/551 (58.98%), Postives = 424/551 (76.95%), Query Frame = 0

Query: 2   EDCISSDEDYYDSDRESLQGLEN---EDSEIQWVSKGPAT-KVITKESLLAAQKEDMRRV 61
           +D +  D+DY + + E+    +N    + ++Q V+   +T +VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 62  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVV 121
           MELLS++E+ ARTLLI+Y+W+VEKLF+V  ++GK  +F+ AG+T+       S +T   +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKKT---M 122

Query: 122 MCDICME-EVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 181
            CD+CME ++ SN  TR++CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 182 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDD--EFCEVECSC 241
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 242 GLQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 301
           GLQFCFSCLS++HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 302 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 361
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 362 AHTDSFKLESKLKESIQEK-ISISEEK-ESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAF 421
           AH DS KLE KL++SI EK +S SE K + + +++SWV + + RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 422 YMFGDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQ 481
           YMFG+ELFKDEM+++EREI+++LFEDQQQQLE NVEKLSK LEEPFD+Y  ++V+++   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 482 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTD 541
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCA---------- 535

Query: 542 KYLPSDCGTSG 544
               SDCG+SG
Sbjct: 543 --ESSDCGSSG 535

BLAST of Cp4.1LG16g02210 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 641.0 bits (1652), Expect = 1.4e-182
Identity = 320/527 (60.72%), Postives = 408/527 (77.42%), Query Frame = 0

Query: 2   EDCISSDEDYYDSDRESLQGLEN-EDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 61
           ++C  S+ D +D      Q   N E+S++Q  S+ P ++VITKE+L+AAQKE + +VME 
Sbjct: 13  DNCYPSEFDDHD------QMCSNAEESDLQH-SREPTSQVITKEALVAAQKEVLVKVMEF 72

Query: 62  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 121
           LS+ E+ ARTLLI Y+W+V+KLF+V  ++GK  LF+ AG+T+ +      S T   + CD
Sbjct: 73  LSVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRAGLTVFD-----PSLTKKTMKCD 132

Query: 122 ICMEEVHSNDA-TRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRN 181
           ICMEE  S  A TR++CGH FCN+CW EHF V+INEG+ +RIRCMA+KCN ICDEA  R 
Sbjct: 133 ICMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNTICDEA--RQ 192

Query: 182 LVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIR--VEDDEFCEVECSCGLQ 241
           LVS    +LA KFDRFL+ESY+EDN  VKWCPS PHCGNAIR   +D +  EVECSCGLQ
Sbjct: 193 LVS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRNIKDDGDVDEVECSCGLQ 252

Query: 242 FCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSC 301
           FCFSCLS++HSPCSCLMW+LW KKC DESETVNW+TV+TK CPKC KP++K  GCN ++C
Sbjct: 253 FCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNTKLCPKCSKPIQKRDGCNHMTC 312

Query: 302 ICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHT 361
            CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ ERA+RDL RY HYH RYKAH 
Sbjct: 313 KCGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQLERAQRDLDRYTHYHYRYKAHI 372

Query: 362 DSFKLESKLKESIQEKISISEE--KESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMF 421
           DS KLE KLK+SI +K  ++ E   + + +++SW+ + + RLFRSRR+LSYSYPF FYMF
Sbjct: 373 DSLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAVNRLFRSRRILSYSYPFVFYMF 432

Query: 422 GDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVIN 481
           G ELFKD+M+ EER I+++LFEDQQQQLE NVE+LSK LEEPFD+Y  ++V+++   + N
Sbjct: 433 GKELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKILEEPFDEYDHEKVVEMMRHLTN 492

Query: 482 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA 523
           L+ + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGIE+A E SA
Sbjct: 493 LTAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGIEQAAEFSA 522

BLAST of Cp4.1LG16g02210 vs. ExPASy Swiss-Prot
Match: Q94981 (E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-1 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 3.7e-63
Identity = 147/448 (32.81%), Postives = 228/448 (50.89%), Query Frame = 0

Query: 39  KVITKESLLAAQKEDMRRVMELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATA 98
           KV+T + ++  Q+E +     LL L     R LL H++WD EKL     +      F  A
Sbjct: 50  KVLTTDEIVQHQREIIDEANLLLKLPTPTTRILLNHFKWDKEKLLEKYFDDNTDEFFKCA 109

Query: 99  GVTMIENRSNC---SSETSSVVMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKI-N 158
            V    N +      +  S    C+IC  ++  +    ++CGH FC  CW E+   KI  
Sbjct: 110 HVINPFNATEAIKQKTSRSQCEECEICFSQLPPDSMAGLECGHRFCMPCWHEYLSTKIVA 169

Query: 159 EGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAP 218
           EG  + I C AH C+ + D+  V NLV+     +  K+ + +  S++E N+ ++WCPS  
Sbjct: 170 EGLGQTISCAAHGCDILVDDVTVANLVTDAR--VRVKYQQLITNSFVECNQLLRWCPSV- 229

Query: 219 HCGNAIRVEDDEFCEVECSCGLQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITV 278
            C  A++V   E   V C CG  FCF+C  + H P  C   + WIKKC D+SET NWI  
Sbjct: 230 DCTYAVKVPYAEPRRVHCKCGHVFCFACGENWHDPVKCRWLKKWIKKCDDDSETSNWIAA 289

Query: 279 HTKPCPKCHKPVEKNGGCNLVSC---ICGQAFCWLCGGATGREHTWS--SISGHSCGRYK 338
           +TK CP+C   +EK+GGCN + C    C   FCW+C G      +W     S ++C RY 
Sbjct: 290 NTKECPRCSVTIEKDGGCNHMVCKNQNCKNEFCWVCLG------SWEPHGSSWYNCNRYD 349

Query: 339 EESEQKA----ERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEEKESMLR 398
           E+  + A    E+ +  L RY+HY+NRY  H  S K E+KL  S+++K+   ++      
Sbjct: 350 EDEAKTARDAQEKLRSSLARYLHYYNRYMNHMQSMKFENKLYASVKQKMEEMQQHNMSWI 409

Query: 399 DFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIRQHLFEDQQQQLEA 458
           +  ++   +  L + R+ L Y+Y FA+Y+             ++  +  +FED Q+ LE+
Sbjct: 410 EVQFLKKAVDILCQCRQTLMYTYVFAYYL-------------KKNNQSMIFEDNQKDLES 469

Query: 459 NVEKLSKFLEEPFDQYTKDRVMDIRMQV 474
             E LS++LE      T + + DI+ +V
Sbjct: 470 ATEMLSEYLER---DITSENLADIKQKV 472

BLAST of Cp4.1LG16g02210 vs. NCBI nr
Match: XP_023513187.1 (probable E3 ubiquitin-protein ligase ARI2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1206 bits (3121), Expect = 0.0
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. NCBI nr
Match: XP_022986492.1 (probable E3 ubiquitin-protein ligase ARI2 [Cucurbita maxima])

HSP 1 Score: 1203 bits (3112), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGK HLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKGHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKAN TDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANNTDKYLPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. NCBI nr
Match: XP_022944387.1 (probable E3 ubiquitin-protein ligase ARI2 [Cucurbita moschata])

HSP 1 Score: 1202 bits (3110), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKG ATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGSATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSERNRLSSLN+DENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNTDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. NCBI nr
Match: KAG7010947.1 (putative E3 ubiquitin-protein ligase ARI1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1196 bits (3095), Expect = 0.0
Identity = 587/593 (98.99%), Postives = 588/593 (99.16%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKG ATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGSATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540

Query: 541 TSG----GLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSG    GLSERNRLSSLN+DENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGKVERGLSERNRLSSLNTDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 593

BLAST of Cp4.1LG16g02210 vs. NCBI nr
Match: XP_038901258.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida])

HSP 1 Score: 1149 bits (2972), Expect = 0.0
Identity = 559/589 (94.91%), Postives = 575/589 (97.62%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           M+DC+SSDEDYYDSDR+SL G+ENEDSEIQWV K PATKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MDDCLSSDEDYYDSDRDSLHGIENEDSEIQWVPKAPATKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGV+MIENRSN SSE SS+VMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVSMIENRSNSSSEPSSMVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVH NDATRVDC HCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNDATRVDCDHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVM+IRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSE  RL+SLNS+ENGCSSRKRARAEVVTGN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. ExPASy TrEMBL
Match: A0A6J1J7N9 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita maxima OX=3661 GN=LOC111484213 PE=3 SV=1)

HSP 1 Score: 1203 bits (3112), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGK HLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKGHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKAN TDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANNTDKYLPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. ExPASy TrEMBL
Match: A0A6J1FVJ5 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita moschata OX=3662 GN=LOC111448849 PE=3 SV=1)

HSP 1 Score: 1202 bits (3110), Expect = 0.0
Identity = 587/589 (99.66%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKG ATKVITKESLLAAQKEDMRRVMEL
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGSATKVITKESLLAAQKEDMRRVMEL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSERNRLSSLN+DENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNTDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. ExPASy TrEMBL
Match: A0A5D3CP89 (Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001360 PE=3 SV=1)

HSP 1 Score: 1143 bits (2957), Expect = 0.0
Identity = 558/589 (94.74%), Postives = 574/589 (97.45%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           M+DCISSDEDYYDSDR+SL GLENEDSEIQ V K P+TKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN SSE SS+VMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVH NDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPFDQYTKD+VM+IRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSE  RL+SLNS+ENGCSSRKRARAEVVTGN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. ExPASy TrEMBL
Match: A0A1S3C9A1 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC103498436 PE=3 SV=1)

HSP 1 Score: 1143 bits (2957), Expect = 0.0
Identity = 558/589 (94.74%), Postives = 574/589 (97.45%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           M+DCISSDEDYYDSDR+SL GLENEDSEIQ V K P+TKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN SSE SS+VMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVH NDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPFDQYTKD+VM+IRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSE  RL+SLNS+ENGCSSRKRARAEVVTGN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. ExPASy TrEMBL
Match: A0A0A0LL32 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1)

HSP 1 Score: 1141 bits (2952), Expect = 0.0
Identity = 557/589 (94.57%), Postives = 573/589 (97.28%), Query Frame = 0

Query: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60
           M+DCISSDEDYYDSDR+SL GLENEDSEIQ V K P+TKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN SSE SS+VMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180
           ICMEEVH NDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCGLQFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPFDQYTKD+VM+IRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589
           TSGGLSE  RL+SLNS+ENGCSSRKRARAEVV GN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 589

BLAST of Cp4.1LG16g02210 vs. TAIR 10
Match: AT2G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 851.7 bits (2199), Expect = 3.6e-247
Identity = 424/597 (71.02%), Postives = 488/597 (81.74%), Query Frame = 0

Query: 1   MEDCISSDED--YYDSDRESLQGLENEDS-EIQWVSKGPATKVITKESLLAAQKEDMRRV 60
           M+D +S +E+  YY SD+ESL G++N++S  I   S+    KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 61  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRS--NCSSETSS 120
           MELLS++EHHARTLLIHYRWDVEKLFAVLVEKGK  LF+ AGVT++EN+S  +  S +SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 121 VVMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 180
           ++ CDIC+E+V     TR+DCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 181 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCG 240
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPS PHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 241 LQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300
           LQFCFSC S AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 301 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 360
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 361 HTDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMF 420
           H DS KLE+KL  +I +K+SISE++E  L+DFSW  NGL RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 421 GDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVIN 480
           GDELFKDEM+ EEREI+Q+LFEDQQQQLEANVEKLSKFLEEPFDQ+  D+VM IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 481 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYL 540
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +  NSK        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 541 PSDCGTSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGN----CFDLNLPAEVVDR 589
            SDCG +  L +   L S  S++  CSS KRAR +    N      DLNLPAE ++R
Sbjct: 541 -SDCGWTSRLDQ--ALESGKSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of Cp4.1LG16g02210 vs. TAIR 10
Match: AT4G34370.1 (RING/U-box superfamily protein )

HSP 1 Score: 847.0 bits (2187), Expect = 8.8e-246
Identity = 415/599 (69.28%), Postives = 497/599 (82.97%), Query Frame = 0

Query: 1   MEDCISSDED--YYDSDRESLQGLENEDSEIQWV-SKGPATKVITKESLLAAQKEDMRRV 60
           M+D  S++E+  YY SD++SL G++NE+SE+Q + SK   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENR-SNCSSETSSV 120
           MELLS++EHHARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  N S   SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++  +  TR+DCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPS PHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL  AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE++E  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINL 480
           +E+FKDEMT EEREI+++LFEDQQQQLE+NVEKLS+FLEEPFD+++ D+VM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERNRLSSLNSDENG--CSSRKRARAEVVTGN----CFDLNLPAEVVDRN 590
            D GTSG  S   + S   S E+    SS+KR + E    N      DLNLPA+ VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 597

BLAST of Cp4.1LG16g02210 vs. TAIR 10
Match: AT4G34370.2 (RING/U-box superfamily protein )

HSP 1 Score: 805.1 bits (2078), Expect = 3.9e-233
Identity = 403/599 (67.28%), Postives = 483/599 (80.63%), Query Frame = 0

Query: 1   MEDCISSDED--YYDSDRESLQGLENEDSEIQWV-SKGPATKVITKESLLAAQKEDMRRV 60
           M+D  S++E+  YY SD++SL G++NE+SE+Q + SK   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 61  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENR-SNCSSETSSV 120
           MELLS++EHHARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  N S   SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 121 VMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 180
           + CD+CME++                  WTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPG----------------WTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 181 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGL 240
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPS PHCGNAIR EDD+ CEVECSCGL
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 241 QFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 300
           QFCFSCL  AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 301 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 360
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 361 TDSFKLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFG 420
           TDS KLE KL+++I EK+S SE++E  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 421 DELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINL 480
           +E+FKDEMT EEREI+++LFEDQQQQLE+NVEKLS+FLEEPFD+++ D+VM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 481 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLP 540
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK    DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 541 SDCGTSGGLSERNRLSSLNSDENG--CSSRKRARAEVVTGN----CFDLNLPAEVVDRN 590
            D GTSG  S   + S   S E+    SS+KR + E    N      DLNLPA+ VD+N
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKEGSFLNNKVTLLDLNLPADFVDQN 581

BLAST of Cp4.1LG16g02210 vs. TAIR 10
Match: AT3G27710.1 (RING/U-box superfamily protein )

HSP 1 Score: 648.3 bits (1671), Expect = 6.0e-186
Identity = 325/551 (58.98%), Postives = 424/551 (76.95%), Query Frame = 0

Query: 2   EDCISSDEDYYDSDRESLQGLEN---EDSEIQWVSKGPAT-KVITKESLLAAQKEDMRRV 61
           +D +  D+DY + + E+    +N    + ++Q V+   +T +VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 62  MELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVV 121
           MELLS++E+ ARTLLI+Y+W+VEKLF+V  ++GK  +F+ AG+T+       S +T   +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKKT---M 122

Query: 122 MCDICME-EVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 181
            CD+CME ++ SN  TR++CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 182 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDD--EFCEVECSC 241
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 242 GLQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 301
           GLQFCFSCLS++HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 302 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 361
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 362 AHTDSFKLESKLKESIQEK-ISISEEK-ESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAF 421
           AH DS KLE KL++SI EK +S SE K + + +++SWV + + RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 422 YMFGDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQ 481
           YMFG+ELFKDEM+++EREI+++LFEDQQQQLE NVEKLSK LEEPFD+Y  ++V+++   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 482 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTD 541
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCA---------- 535

Query: 542 KYLPSDCGTSG 544
               SDCG+SG
Sbjct: 543 --ESSDCGSSG 535

BLAST of Cp4.1LG16g02210 vs. TAIR 10
Match: AT3G27720.1 (IBR domain containing protein )

HSP 1 Score: 512.7 bits (1319), Expect = 4.0e-145
Identity = 265/484 (54.75%), Postives = 339/484 (70.04%), Query Frame = 0

Query: 43  KESLLAAQKEDMRRVMELLSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTM 102
           +ES L   +E   +VME LS+ E+ ARTLLI Y+W+V+KLF+V  ++GK  LF+ A    
Sbjct: 31  EESDLQHSREPTSQVMEFLSVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRAEEEE 90

Query: 103 IENRSNCSSETSSVVMCDICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIR 162
            E++                 EE    D    + G     +   E  +    E +  +  
Sbjct: 91  GEDKEEEEGGEDE-------EEEEGGEDEEAEEGG----EDEEAEEGVEDEEEEEDEK-- 150

Query: 163 CMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIR- 222
                     D+ V   LVS    +LA KFDRFL+ESY+EDN  VKWCPS PHCGNAIR 
Sbjct: 151 ----------DDEV--QLVS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRN 210

Query: 223 -VEDDEFCEVECSCGLQFCFSCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCP 282
             +D +  EVECSCGLQFCFSCLS++HSPCSCLMW+LW KKC DESETVNW+TV+TK CP
Sbjct: 211 IKDDGDVDEVECSCGLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNTKLCP 270

Query: 283 KCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAK 342
           KC KP++K  GCN ++C CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ ERA+
Sbjct: 271 KCSKPIQKRDGCNHMTCKCGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQLERAQ 330

Query: 343 RDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEE--KESMLRDFSWVNNGLCRLF 402
           RDL RY HYH RYKAH DS KLE KLK+SI +K  ++ E   + + +++SW+ + + RLF
Sbjct: 331 RDLDRYTHYHYRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAVNRLF 390

Query: 403 RSRRVLSYSYPFAFYMFGDELFKDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPF 462
           RSRR+LSYSYPF FYMFG ELFKD+M+ EER I+++LFEDQQQQLE NVE+LSK LEEPF
Sbjct: 391 RSRRILSYSYPFVFYMFGKELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKILEEPF 450

Query: 463 DQYTKDRVMDIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKAL 522
           D+Y  ++V+++   + NL+ + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGIE+A 
Sbjct: 451 DEYDHEKVVEMMRHLTNLTAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGIEQAA 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84RR25.1e-24671.02Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q949V61.2e-24469.28Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVX08.5e-18558.98Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVW91.4e-18260.72Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q949813.7e-6332.81E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-... [more]
Match NameE-valueIdentityDescription
XP_023513187.10.0100.00probable E3 ubiquitin-protein ligase ARI2 [Cucurbita pepo subsp. pepo][more]
XP_022986492.10.099.66probable E3 ubiquitin-protein ligase ARI2 [Cucurbita maxima][more]
XP_022944387.10.099.66probable E3 ubiquitin-protein ligase ARI2 [Cucurbita moschata][more]
KAG7010947.10.098.99putative E3 ubiquitin-protein ligase ARI1 [Cucurbita argyrosperma subsp. argyros... [more]
XP_038901258.10.094.91probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1J7N90.099.66probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FVJ50.099.66probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A5D3CP890.094.74Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C9A10.094.74probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC10349843... [more]
A0A0A0LL320.094.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G16090.13.6e-24771.02RING/U-box superfamily protein [more]
AT4G34370.18.8e-24669.28RING/U-box superfamily protein [more]
AT4G34370.23.9e-23367.28RING/U-box superfamily protein [more]
AT3G27710.16.0e-18658.98RING/U-box superfamily protein [more]
AT3G27720.14.0e-14554.75IBR domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 260..325
e-value: 0.012
score: 23.3
coord: 190..252
e-value: 5.5E-21
score: 85.7
IPR002867IBR domainPFAMPF01485IBRcoord: 191..252
e-value: 1.9E-12
score: 47.2
coord: 268..310
e-value: 1.1E-5
score: 25.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 113..204
e-value: 3.5E-18
score: 67.3
NoneNo IPR availableGENE3D1.20.120.1750coord: 248..496
e-value: 5.6E-63
score: 215.0
NoneNo IPR availablePANTHERPTHR11685:SF345RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 6..516
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 191..259
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 260..325
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 114..202
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 6..516
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 119..167
score: 9.848491
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 115..329
score: 45.123802

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g02210.1Cp4.1LG16g02210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity