Cp4.1LG16g01540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g01540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptiontRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
LocationCp4.1LG16: 3573953 .. 3583377 (+)
RNA-Seq ExpressionCp4.1LG16g01540
SyntenyCp4.1LG16g01540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCTTCGGAAGGGAAATATTTATGTGAAAAACCTTATCCAAATCCGGAAGGATTTCTTCTGCTGCGTAAAACTGAAGGGCAAATATTCGACCAAGCCGGCGGCGGCGATGGGCTACGCAGCCATCGTCATCGTTAGGTTATGAGATCCCCTTCCAATTCTCACCTCCATCTCAAATCTATATTGTCACTCCGTAAGTCATTCATGGCTTCCTCTCGCGCACAAACCGATCTCCCTCAAGACCTCGACGACCTTCTCTGCTCCGATCCACACACACAGCGACAAGATGAATGCTCCTCTCTTACCAAATCCAATGACTCAACACTTCTCTTGGGGTCGCCGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGTCCAAGCTCGGTCGGCCGAAGTTGATTGTAGCTCCGATGGTTGATAATTCGGAGCTTCCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACACCTATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGTAACATGGAATTCACAACTTGTCAGGTATGTTAAACGTTATAAGTCATTCTTTTGTGTTAACAACTTGAGTTTTCTGTTTTAGGATATAATCTTGTCTGTATTTCTGTAAGTCTATTCTTCATTGTTCTTTAAACTTTGGAATGATTCGTAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTATGTCGATCTTAATCTCGGGTAATCTCTATTAGTCTAATTCGTATTCGAAGGGTAATTTGTACGAATATTTTCATTCCATATGTTCTTATATTCAAAATGCTGACCTCTTATACTTCTTGTGGGTTGGTGGAGTAAGGATTTGTCTTCCAAAAGAGTTCTTTGAATAAGAAACAATTTCATTGATGGTATGAAATTACGAAAGAAATAGCCTCCTTCTCTGATTAGCGAGCAGGGAAGGAAAACTATACTAATGAAAAAGAGGACGCATTTACACCAAGTTGCAACCAAACTAACTATAGCCTCAAAAAATCTAAGTTATGTGAGTTCCTTATCGTGAAAGATTCTTCTATTACGTTCTTGCCAAATGGTCAGTAAATGCTCCATATTGGTCAGTAATATGTAGGGGAGGTTTGATAGGGTTGCTTATACGAGTGTAAGTCTACCCCCTTGAGATGCGTTTGTTGGTCCAGATTGGTGGACGTTGATGGGGGACGTTCCGAGGGTGAAGCCTATAATGGCACTTATTTATTCAACATGAATAAAAAATTTACTAAAACAACAACCATCCTAATGAAAAGAAAAGGGGGAAAGCTGGTCACCTTGGCGATGACCTCCAGTAGCAAGAATCTCTCCTCTTGCTTCGCTGTTAATGATAATTGGAAAATTAGGAGAGCAGATGAGACCCGTTATCCATTTCCTCCATGTGTGGCTGGAGCTTTTTGCCAATAGGATCTCGTCTAAAAAATCCCAGCCCACCTTGTTCAAAGCTTTCTCAATGCCTAGCTTAAGAACTGCCTCTTTTTTCACTCTCCTTTTCCACCCATCCATAAAATCATTTGCAATAAGGTGGCATCAAGGATTTGTCTACCACCCACAAAAGTCGATTGGTATTCGGTGATAATATGGGGTAGAACCTTCTTGAGAGACTCGGATAGAACTCAAGCAATGATCTTATACATACAAGAAATAAGGCTAGTGGGGTGAAACTAGCCAACAGTTTGGGCATTTGTTTTCTTGGGGATGAGGCATATGTAAGCCTTATTCAAGCTGGCATTGTAATCCCATTCTTGAAAAAATCATGGAACACTGTTCCAAGACTTCTAGAATTCAACTGTAAAACCATCCAGCCTCATTAGCACTCAATTCTTTTACAGAAGACCAAATTTCTTCTTTTGTAAAGGCCATCTCCAATTCCAATGCTTGTTGAGTGGTAATAGGGTCCCAATAAATAGGAGATGGAAAAAACTGGCACCCTTCTTTTTGGTAAAAAAATCCACATAAAATATAAGAAATGCTTCTTCAATCTCCGTTTCAGTGACACTGTAACCATCCTTGGAAAGAATCTTTAAGATACATATATTTTTTTACTTCTTGAGGTCACAATCCAATGAAAGAAGCTTGTGTTGATATCTCCTTCAATCAACTATTTCAATTTACAGCTTTGTCTTCACATGTTTTCTTCTTGGATAGCCAAGGATAAAAGCTAGGTTTTGATAGAAGTCTTCTGAGTGATTTGGATATGATGGATTTCCCCTGTTTCTTCAATGGAGTCCAAAACTAAGAACTCCATGGCAGGATTTCCTTCTAAATTTTGATGCTGCTAGAGGTTTCCTTGTTCCATGTTCTTTAACTGATTTGAGGCTCTCAAGTTTTGAATAAAGTCATGATCTGGCCAACCTCTCAAAAAATGTATTCAGCCACCAATATTCAACCTTAGAAAGAAAGCTTTGATGTTGCGTCCACATATTTTGGAATCTAAAATGAGTATGGCCTCATTTATCCACCCCAAAAGAAAGTTGAACGGGATAATGATTGAAACAATTCTTTCTAGTCTTTTAACCGTAGCAATAGGGAATCTGTTAAGTGGTCGATGACAAGGAATATGTCAATAATCGTGAGAGTGGGAGACTCCCTAAGATCTAATCATGTGGCAGCCCATTTGTCATTGGAATCTCAGTAAGGGCCGCATGCTTTGCCTTGCGGTTCTTCAAGCATTATAATTTTCTTTTGAAGAGCATATTGTTGAAATACCCTCCCAAAAGCCATGATTCAGTGCGTTAAAAGGTCTGGTACCATAATTCCTCAGGCATAAATTCCCCATGAGTCAGTGTTCGATTTCCACAAACTAGTAATCGATGCTTGTTTTGTGAGGGAGAGATGGCAAAGAACAGGATATTTAGTTTGGACATTTAACGACCTAGACCTAGCTTTTTACTGACCTAGAGTCATTGCCATATGCATGACGTAACTTTTTTATGCGGAATGATTCATCTAAAACAACTTCATAAAACAATGTCAGTGACTTAAACGTTTAAGAAAAGGGTCTTATAACAATACAAATGAAATATTCAAAATAAATTAAAATAAACTAAGGAAAATATTAAAATAACAATGCTCTATCCTAACCTAAGATCTATGAAAATAAATGCAACTACCTATGCAAGTTCATTAGTCTTTGGTGCGATGCTGTCATCACCGGTACATGGCTTGAGAAATATTCAGTAAGTGACCCCACTATTAGGAATGCAAACCCATGCAATCAATAATGAGGACCTATCATTTAGAACCATTGTCTCTTAGGTAGCCTCTAGTCCGGACAAACTGAATATGTAGTACTTCCCTACACATGACCCACATGTGCGAATGTGGGCCCACCAGGCGGGCTCACACACCTGCTGGGCCTGCATGGTCGGGCTAGTTGGCTATAGCGGACGAGGTACGACGACGTCGTGTATCATGCCAAGCACGATAGTCATCATCTTCATAGTGTACACGTCCATACTCATCATAAATTGGGAAGTATCTCGATGTAAATGGACATGCAATATGCATGAGGATACTTAATCATCTTAATCATTCATGCGGCACAATCCCCGCACATTCTTTACATATTACCCTAGGTAACATGCATGCATAAGCTTTCATACATTCATCATTCATAACATTACTTGGGAGTTTTGCATCAGAAAATTCTTCATTGTCATACATGTCGTAGCATGTCATATCATAATGCAACATGACTTCTCATACATGCATATCATCGTAACATGAGATATCATCATAATATATCATATCGTCATACATTTCATGTCATTGTAACATATTACATCATCGTCATAATGTTTCATAACATCATAGCATATCACATCATCATAACATTTCATAGCATCATAGCATATCATATCATCATACCACATCATATCAACATACATGACACGTGCAGGGGCCACTGGGCACATGTCATCATACTTAAGACAAGTATTCCGACCGAGTTGATAGTAGGGCCACTTGGTTGGTCTAGGTTGAAACTACACCCCTTGATTCTAGTTTAACGCTGCTCTCTGAAATGTAATTCTACCTAACGAGTCCCACAATAATAAATTAGAATTCTATTGTGTGAATTGTCTCACCTAATCTTGCATAAAATAATTTAAACCAGCTAAACCTAATTTATAATTTAAACTAGCTATACCTAATTTTGCATAAAATAATTTGCTGGAAGGTAGTCAAACTAACTTTAAATGCTTCTAAATAGGAAAGTGTTCGAGCATATCAGACCCAAGCCAGATCAGTCAAAACGTTGGCATGTAGCCGTCAGATAATGTATTCTCCGATTGGCTTCGAACTGTTCAAGTGGGATGAAAGGGAGTTAAGGACCTTCTTTTTATGGTAGAGTCTAACTCCCCACTTTTCAACATAAGCAATGTGGGACAACTTCCTTTTTTTTCTTGGGTTATTAAATCATACACATTTAAAATACTACATCATAACATGTGCGACGTATCCATGCTTCACAAATACTTGGATGACTCGTCTCACGTCGTAGATTACAAGTTCTTATAGATAGACGATAGCCTAAGATACGAGAAAAAGCCAATAAGAATTTTATCTCGGGAAGTGAAGGTCTTCTGTACTAGGAATATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAATTTAAACCAGCTAAACCTAATTTATAATTTAAACTTATTCCTAATAATTTAAACTAGTTATACCTAATTTTGCATAAAATAATTTGCTGAAAGGTAGTCAAACTAACTTTAAATGCTTCTAAAGAGTTGAAATTAGTAGGAAAGTGTCCGAGCATATCAGACCCAAGCTAGATCAGTCAAAACATTGGCATGTAGCCGTCAGATAATGTATTCTCCGATTGGCTTCGAACTGTTCAAGTGGGATGAAAGGGAGTTAAGGACCTTCTTTTTATGGTAGAGTCTAACTCCCCACTTTTCAACATAAGCAATGTGGGACAACTTCCTTTTTTTTCTTGGGTTATTAAATCATACACATTTAAAATACTACATCATAACATGTGCGACGTATCCATGCTTCACAAATACTTGGATGACTCGTCTCACGTCGTAGATTACAAGTTCTTATAGATAGACGATAGCCTAAGATACGAGAAAAAGCCAATAAGAATTTTATCTCGGGAAGTGAAGGTCTTCTGTACTAGGAATATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTCTCACATTGCTCATGTTTAAAAGTTGGGGAGTTAGGTGAAGGTCCCTTACTTCCTTTCATCCCACTTGAACGATTCGAAGTCAGTCGGAGAATATATTTTTTGACGGCTTCAAGCTAACATTTTGGCTGATCTGGCTTGGGTCTGATGCGCTCAGACCCTTTCCTACTAGTTTCAACTCTTCGGAAGTGTTTAAGGTTAGTATGACTATCTTTTAGTTAGTTTAAATTTTTGGAGTCAAGTTTAAATTATTTTATGCAAAATTTGGTTTAGCTAGTCTCAATTATTAATTAGGTTTAGCTAGTTTAAATTACTAAGAGTATGTTTAAATTGTTTCATGCAAAATTAGGTGAGCCAATTCTCATCTTAAAATTCTATTTTAGGATTGTAAGAATCCTTAGGTAGAATTACATTTTAGAGAGTAGAATCCTTCGGTAGAATTACATTTTAGAGAACAAAATCCTTAGGTAGAATTACATTTTAGAGAGAAGAATCTTTAGATAGAATTACATTTTAGAAAGCAGAATCCTCCAGTAGAATTACATTTTAGAGAGCAAAATCCTAGGCCAACCAAGTAAGTGGCCCTGCTATCAGTTCGGTCGGAAGACTTGTGTTGTGTATGATGACACGTGCCTAGTGGCTCATGCACATGTCATATATGTTGATATGTTATGATGATTTGAAGTGTTATGATATGTTATGATGATGATGTAACACTATGATGACATGAGATGTATGAGGATATGACATAGTATGATGATATCTCATATTACGATGATATGCATGTATAAGATGTCATGTTGCACTATGATATGACATGTCTGGCAATGAAGAGTTTTCTAATGCAACCCTTGAGTAATGTTATGAAGCTTATACATGCAAGTTTCCTAGTGAGTAATGTTATGAAGCTTATACATGCAAGTTTCCTAGGGTAATATGTGAGGTTATGCTGCATGAATGATTAAGATGAAAAGTATAAGGACTTCATGCATATTGTATGTCTATTTACATCGGGATACTTCTCCTTTTATGATAAGTACAAACACATATACTATGATGATGACGACTATAATACTTGGCATGATACATGTTGTCGTACTTCTGGACGTTCGGTTACGACTATATATCCCGACCATGCAGGCCCAGGGGGTGTGCGAGCCCACCTGGTGGGCCCACGTTCGCATATGTGAGTCGTGTGTAAAGAAGTACTACACATCCAATTTATTCGGACTGGAGGCTACTTAAGAGACTATAGTTTTAAATGATAGGTCTCTATTCATTAGTATTTTAAGAAATACTCAAGTCATGTGCTATTATTATATTTCAGGTAAATGCAAAGGCGCACCAAAAACCATGAAACTTGCATAGGGTAGTTGCATTTATTTTCGTAGATCTTAGATTCGTACAGGGCATTGTTATTTTTATATTTTCCATGGCTTATTTTAATTTACTTTGAATATTTTCTTTGTATTGTTTTAAAACCATTTTCTTAAACGTATAAGTCTATGACATTGTTTTATAAATTTGTTTTAAATGAATATTTCTGTATATAGAAGTTACGTCATGCATATGGTAACGACTCTAAGTCCCTAGAAAACTAGTGTCGTTACATTTTCAATAGACTTCGGTAGACCTCTTGAGGAGAAATAATGATTTCCTACCCGTTTTTTAGTTGAAATGAGTAGTTCCATATTTTGGAGCAATAACTCTTCTCCAAAGGGCCTCCTTCTCATGATAAGAACGCCAAATCCACTTTGCTAGGAGGTAGATGTTTTTCCTTATGAAGATCATATATCCTCAGGCCTCCTTCAATAGGCTTTTCTAAAGATCATTTAACAATACCTTTCTTGTCAGTATTGTGTTTCTCAGAAATATTAACGGCTATATTTGGGCTATTTTGAAACGACGATGGTTCAGAGATAGTTAAGGAGAGAGAAGGCAGAACAGATTGGGTAATGTTTGGTTTGTGGGGCTTTTGGTTTGGTTTAGACTTATACGTTTAGTTTAAAACAAGAAATAGAAATAACGTCGGTCAAAATAAAACCTTAACATAATGTTTGAGTACGAAAATGAAAAACCACCTATAAAACTACAAACGGAAAACGTTGCATACTACTATCGTATCTTAGCTTCCATGGCCATTCCAACATTCCCCCTTATCCATGTAGAGGCGCTTTACCTTTACTTGAAATGTAATATCTTAGCTTCCATGACCATTCCAACATTCCCCCTTATCCTTATCCATGCAGAGGCGTTTTACCTCTACCTGAAATGTAAGAGTAGCGCATGGCTGGAGTATTTTAAGAAACGTTTTAGTAAGTAACCTCATTGTTGTGGTTGGGTAATGCAAGCGCACACATACATGATAAGACCTATCTCTTAAAACAACATAGCATGACCTTAAACTCTTTTCAATTTCGGGCAGGGTTGGATGTATGGTACTTCCCCACACATGGCCCGACATTGCCCGGAATTAGAAAGAGTTCAAGGCCATGCTATATTGTTTTAAAGGATAGGTCCCATCATGTATGTGTGTGTGCTAGACCCAACCCCAACAATTGGGTTACTTAGTATTTATTAAAATATTCAAACTATGTGTTACTCTTACATCTCAGTAAAGCGTCCCCTTCCTGTTAGTATTTATTCTTTTTATTTATTTTCTGCATTGTTCTCCATTTCAGTTGCGTTTTTAACTAAAAAAACAATCAAGAACAATCGTAGTTCACGTGCTTTCCATATGTGTCGCTTCCAAATATGTTGATGGTATCCATATTTTTCACTTACGGTTTCTGAACTTCATTTGAGTGCTCTATTAATTTCATGCAGTTGTCCTCAGCGTATTGCAAGGCGAGGAAACTATGGGGCTTTTTTGATGGACAACTTACCTCTTGTAAAAGCAATAGTAGAGAAAGTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAGTCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGCAAAAAATTCCGAGCTAATTGGAGCGCCATTAGTGCTGTTAAAAACGCTCTACGAATCCCGGTACTTGCCAATGGGAACATAAGGCACATGGAAGATGTTCACAACTGTTTGCAAGAGACTGGAGTCGAGGGCGTGCTTTCAGCCGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACCGCGCAATGGACAGCGGGCTGTGAAGAAAGTAGCAGGGATGGAAACCTGGATCAAGCTGATCTACTGGTGGACTACTTGAAGCTTTGTGAAAAATATCCTGTCCCGTGGCGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAACATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATTAAGGATTCTCCTGCATCTGCTATTTCATCCATTGGTTGACTGCTTCACTGATCATTGATTGATAGGCTTACTGGTCTTAACTTAAAAATTCTTTGCCAAAATTAGATTCATTCTGTTGTTGTACCATTGATATTCACATAAAACTGTTCTTCAATCAGCTTGGACACAGCGATGAAAGAAAAGATTTTGCATATTGTGAATTGTAGCCTGAATTTATTTGATTAGGGAAGAGACAGAGAGTTAACGTTACCTTTTGGCTTTGAATTGTGAGTAGTGATTTTAACTATGCAAAATGCATGTCAACTCAAAGTCACCCACTTATTTCATCTTATTGTTTGAACATTGTTTCGTTGCCATGATAGCTTGATTTGATACGAGACTCGTT

mRNA sequence

TATCTTCGGAAGGGAAATATTTATGTGAAAAACCTTATCCAAATCCGGAAGGATTTCTTCTGCTGCGTAAAACTGAAGGGCAAATATTCGACCAAGCCGGCGGCGGCGATGGGCTACGCAGCCATCGTCATCGTTAGATCCCCTTCCAATTCTCACCTCCATCTCAAATCTATATTGTCACTCCGTAAGTCATTCATGGCTTCCTCTCGCGCACAAACCGATCTCCCTCAAGACCTCGACGACCTTCTCTGCTCCGATCCACACACACAGCGACAAGATGAATGCTCCTCTCTTACCAAATCCAATGACTCAACACTTCTCTTGGGGTCGCCGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGTCCAAGCTCGGTCGGCCGAAGTTGATTGTAGCTCCGATGGTTGATAATTCGGAGCTTCCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACACCTATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGTAACATGGAATTCACAACTTGTCAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTATGTCGATCTTAATCTCGGTTGTCCTCAGCGTATTGCAAGGCGAGGAAACTATGGGGCTTTTTTGATGGACAACTTACCTCTTGTAAAAGCAATAGTAGAGAAAGTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAGTCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGCAAAAAATTCCGAGCTAATTGGAGCGCCATTAGTGCTGTTAAAAACGCTCTACGAATCCCGGTACTTGCCAATGGGAACATAAGGCACATGGAAGATGTTCACAACTGTTTGCAAGAGACTGGAGTCGAGGGCGTGCTTTCAGCCGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACCGCGCAATGGACAGCGGGCTGTGAAGAAAGTAGCAGGGATGGAAACCTGGATCAAGCTGATCTACTGGTGGACTACTTGAAGCTTTGTGAAAAATATCCTGTCCCGTGGCGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAACATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATTAAGGATTCTCCTGCATCTGCTATTTCATCCATTGGTTGACTGCTTCACTGATCATTGATTGATAGGCTTACTGGTCTTAACTTAAAAATTCTTTGCCAAAATTAGATTCATTCTGTTGTTGTACCATTGATATTCACATAAAACTGTTCTTCAATCAGCTTGGACACAGCGATGAAAGAAAAGATTTTGCATATTGTGAATTGTAGCCTGAATTTATTTGATTAGGGAAGAGACAGAGAGTTAACGTTACCTTTTGGCTTTGAATTGTGAGTAGTGATTTTAACTATGCAAAATGCATGTCAACTCAAAGTCACCCACTTATTTCATCTTATTGTTTGAACATTGTTTCGTTGCCATGATAGCTTGATTTGATACGAGACTCGTT

Coding sequence (CDS)

TATCTTCGGAAGGGAAATATTTATGTGAAAAACCTTATCCAAATCCGGAAGGATTTCTTCTGCTGCGTAAAACTGAAGGGCAAATATTCGACCAAGCCGGCGGCGGCGATGGGCTACGCAGCCATCGTCATCGTTAGATCCCCTTCCAATTCTCACCTCCATCTCAAATCTATATTGTCACTCCGTAAGTCATTCATGGCTTCCTCTCGCGCACAAACCGATCTCCCTCAAGACCTCGACGACCTTCTCTGCTCCGATCCACACACACAGCGACAAGATGAATGCTCCTCTCTTACCAAATCCAATGACTCAACACTTCTCTTGGGGTCGCCGAGTGGATATTTGAGTGGCGAGTCGCGCATTGAGCGAGCCTGGGCTCACTGGTCCAAGCTCGGTCGGCCGAAGTTGATTGTAGCTCCGATGGTTGATAATTCGGAGCTTCCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACACCTATGCTGCATTCACGGATCTTTACTGAGAACGAGAAGTATCGTAACATGGAATTCACAACTTGTCAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTATGTCGATCTTAATCTCGGTTGTCCTCAGCGTATTGCAAGGCGAGGAAACTATGGGGCTTTTTTGATGGACAACTTACCTCTTGTAAAAGCAATAGTAGAGAAAGTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAGTCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTCCATGGCCGCACAAGAGATGAAAAAGATGGCAAAAAATTCCGAGCTAATTGGAGCGCCATTAGTGCTGTTAAAAACGCTCTACGAATCCCGGTACTTGCCAATGGGAACATAAGGCACATGGAAGATGTTCACAACTGTTTGCAAGAGACTGGAGTCGAGGGCGTGCTTTCAGCCGAAACCCTTCTGGAGAATCCTGCACTTTTTGCTGGGTTTCGAACCGCGCAATGGACAGCGGGCTGTGAAGAAAGTAGCAGGGATGGAAACCTGGATCAAGCTGATCTACTGGTGGACTACTTGAAGCTTTGTGAAAAATATCCTGTCCCGTGGCGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGATTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAACATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATTAAGGATTCTCCTGCATCTGCTATTTCATCCATTGGTTGA

Protein sequence

YLRKGNIYVKNLIQIRKDFFCCVKLKGKYSTKPAAAMGYAAIVIVRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Homology
BLAST of Cp4.1LG16g01540 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 4.9e-79
Identity = 143/298 (47.99%), Postives = 194/298 (65.10%), Query Frame = 0

Query: 128 WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 187
           WS+ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRALGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 188 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 247
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGRYGAFLQEEWDLL 129

Query: 248 KAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 307
           + ++      L VPV+CKIRVFP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 308 NWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQ 367
           +W  I AV+ A+ IPV ANGNIR ++DV  C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 368 WTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 423
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELADEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Cp4.1LG16g01540 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 8.4e-79
Identity = 142/298 (47.65%), Postives = 195/298 (65.44%), Query Frame = 0

Query: 128 WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 187
           WS+ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 188 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 247
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQEEWDLL 129

Query: 248 KAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 307
           + ++      L VPV+CKIRVFP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 308 NWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQ 367
           +W  I AV+ A+ IPV ANGNI+ ++DV  C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 368 WTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 423
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Cp4.1LG16g01540 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.2e-78
Identity = 142/298 (47.65%), Postives = 194/298 (65.10%), Query Frame = 0

Query: 128 WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 187
           WS+ L   + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLRGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEV 69

Query: 188 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 247
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL D   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLL 129

Query: 248 KAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 307
           + ++      L VPV+CKIRVFP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAA 189

Query: 308 NWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQ 367
           +W  I AV+ A+ IPV ANGNI+ ++DV  CL++TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 368 WTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 423
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREE 291

BLAST of Cp4.1LG16g01540 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 6.0e-69
Identity = 132/302 (43.71%), Postives = 194/302 (64.24%), Query Frame = 0

Query: 128 WSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFT--TC 187
           ++K+GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E+E YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 188 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 247
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLGCPQ IA++G YG+FL +N  L++
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIE 131

Query: 248 AIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 307
           +I+ K+ + L +PV+ KIR+FP+ Q T++YA+M+  AG S+LAVHGR R++K      A+
Sbjct: 132 SIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIAD 191

Query: 308 WSAISAVKNAL--RIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTA 367
           W  I  ++  L     + ANGNI H +D+  C++ TGV+GVLSAE  L NP +F    + 
Sbjct: 192 WEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLPPSSP 251

Query: 368 QWTAGCEESSRDGNLDQADLLVDYLKLCEKYPV-----PWRMIRSHVHKLLGDWFKIHPH 421
             T           +D  D+  +YL +  ++ +         I+ H+ KL+     IH  
Sbjct: 252 LMTL-------YPRID--DMCEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTD 304

BLAST of Cp4.1LG16g01540 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.0e-64
Identity = 129/315 (40.95%), Postives = 190/315 (60.32%), Query Frame = 0

Query: 128 WSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTT--- 187
           + K+GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  ++KYR   +++   
Sbjct: 23  FDKIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDG 82

Query: 188 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 247
              DRPL VQFCANDP+ LL AA+ VE  CD VDLNLGCPQ IA++G+YG+FLM+   L+
Sbjct: 83  SSVDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLI 142

Query: 248 KAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 307
             ++  +  NL+VPV+ KIR+F + + ++NYA+M+ DAG   L VHGR R++K  K   A
Sbjct: 143 HNLINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLA 202

Query: 308 NWSAISAVKNAL--RIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRT 367
           NW  I  +++ L       ANGNI + ED+  C++  G + V+SAE  L NP +F   +T
Sbjct: 203 NWETIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT 262

Query: 368 AQWTAGCEESSRDGNLDQADLLV----DYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 427
                     +++    + D ++      +K C++       ++SH  K+L  +   H  
Sbjct: 263 ---------KNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTD 322

Query: 428 IREDF-NAQSKLTFE 433
           IR       +K T+E
Sbjct: 323 IRSTLATMNAKATWE 328

BLAST of Cp4.1LG16g01540 vs. NCBI nr
Match: XP_023512932.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855 bits (2209), Expect = 1.82e-312
Identity = 419/420 (99.76%), Postives = 420/420 (100.00%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. NCBI nr
Match: XP_022944464.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 845 bits (2182), Expect = 2.38e-308
Identity = 414/420 (98.57%), Postives = 416/420 (99.05%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASS  QTD PQDLDDLLCSDPHTQ QDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQTDFPQDLDDLLCSDPHTQPQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. NCBI nr
Match: XP_022986543.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 845 bits (2182), Expect = 2.38e-308
Identity = 414/420 (98.57%), Postives = 417/420 (99.29%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASS AQTD PQDLDDLLCSDPHTQRQDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQTDFPQDLDDLLCSDPHTQRQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAI AVKNALRIPVLANGNIRHMEDV+NCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. NCBI nr
Match: KAG7011024.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 844 bits (2180), Expect = 4.79e-308
Identity = 414/420 (98.57%), Postives = 415/420 (98.81%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASS  QTD PQDLDDLLCSDPHTQ QDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQTDFPQDLDDLLCSDPHTQPQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTI YARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTITYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. NCBI nr
Match: KAG6571229.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 805 bits (2080), Expect = 3.74e-293
Identity = 394/399 (98.75%), Postives = 394/399 (98.75%), Query Frame = 0

Query: 66  MASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDSTLLLGSPSGYLSGESRIERAW 125
           MASS  QTD PQDLDDLLCSDPHTQ QDECSSLTKSNDSTLLLGSPSGYLSGESRIERAW
Sbjct: 1   MASSFTQTDFPQDLDDLLCSDPHTQPQDECSSLTKSNDSTLLLGSPSGYLSGESRIERAW 60

Query: 126 AHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTC 185
           AHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTC
Sbjct: 61  AHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTC 120

Query: 186 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 245
           QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK
Sbjct: 121 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 180

Query: 246 AIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 305
           AIVEKVASNLQVPVSCKIRVFPNLQDTI YARMLEDAGCSLLAVHGRTRDEKDGKKFRAN
Sbjct: 181 AIVEKVASNLQVPVSCKIRVFPNLQDTITYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 240

Query: 306 WSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQW 365
           WSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQW
Sbjct: 241 WSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFAGFRTAQW 300

Query: 366 TAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNA 425
           TAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNA
Sbjct: 301 TAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNA 360

Query: 426 QSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           QSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 QSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 399

BLAST of Cp4.1LG16g01540 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 845 bits (2182), Expect = 1.15e-308
Identity = 414/420 (98.57%), Postives = 417/420 (99.29%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASS AQTD PQDLDDLLCSDPHTQRQDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQTDFPQDLDDLLCSDPHTQRQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAI AVKNALRIPVLANGNIRHMEDV+NCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 845 bits (2182), Expect = 1.15e-308
Identity = 414/420 (98.57%), Postives = 416/420 (99.05%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQTDLPQDLDDLLCSDPHTQRQDECSSLTKSNDS 104
           +RSPSNSHLHLKSILSLRKSFMASS  QTD PQDLDDLLCSDPHTQ QDECSSLTKSNDS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQTDFPQDLDDLLCSDPHTQPQDECSSLTKSNDS 60

Query: 105 TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 164
           TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP
Sbjct: 61  TLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTP 120

Query: 165 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 224
           MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP
Sbjct: 121 MLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCP 180

Query: 225 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 284
           QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC
Sbjct: 181 QRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGC 240

Query: 285 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 344
           SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV
Sbjct: 241 SLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGV 300

Query: 345 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 404
           LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH
Sbjct: 301 LSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMIRSH 360

Query: 405 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAISSIG 464
           VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDSPASAISSIG
Sbjct: 361 VHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAISSIG 420

BLAST of Cp4.1LG16g01540 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 775 bits (2001), Expect = 5.47e-281
Identity = 387/424 (91.27%), Postives = 401/424 (94.58%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQT---DLP-QDLDDLLCSDPHTQRQDECSSLT- 104
           +RS SNSHL+LK+I SLR SFMASS  QT   DLP QDLDDLLCS P  QRQDE SSLT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 105 -KSNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 164
             SNDS LLLGSP+GYLSGESRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 165 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 224
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 225 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARM 284
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 285 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQE 344
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV+NCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 345 TGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPW 404
           TGVEGVLSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 405 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAS 462
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS   
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

BLAST of Cp4.1LG16g01540 vs. ExPASy TrEMBL
Match: A0A0A0LTG2 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 4.09e-276
Identity = 384/424 (90.57%), Postives = 396/424 (93.40%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQT---DL-PQDLDDLLCSDPHTQRQDECSSLTK 104
           +RS SNSHLHLK I SL  S MASS  QT   DL PQDLDDLLCS  H Q Q E SSLT 
Sbjct: 1   MRSLSNSHLHLKYIFSLPNSLMASSLIQTLKPDLAPQDLDDLLCSPSHKQPQHESSSLTN 60

Query: 105 S--NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGA 164
           S  NDST LLGSP+GYLS ESRIERAWAHW+KLGRPKLIVAPMVDNSELPFRLLCRKYGA
Sbjct: 61  STSNDSTPLLGSPTGYLSRESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 165 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 224
           EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 225 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARM 284
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 285 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQE 344
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV++CLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNDCLQE 300

Query: 345 TGVEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPW 404
           TGVEGVLSAETLLENPALFAGFRTA+W  G EE++RDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGHEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 405 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAS 462
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS A 
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHAL 420

BLAST of Cp4.1LG16g01540 vs. ExPASy TrEMBL
Match: A0A6J1D4R6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3673 GN=LOC111017278 PE=4 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 8.58e-275
Identity = 367/423 (86.76%), Postives = 400/423 (94.56%), Query Frame = 0

Query: 45  VRSPSNSHLHLKSILSLRKSFMASSRAQT---DLPQDLDDLLCSDPHTQRQDECSSLTKS 104
           +RS + SHLHLKS+ SL  S MASS +QT   DLP+ LDDLLCS+   Q+++ECSS+TKS
Sbjct: 1   MRSLAISHLHLKSVFSLHSSLMASSFSQTIKPDLPEHLDDLLCSNQQQQQREECSSVTKS 60

Query: 105 NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 164
           ++ +LLLGSP+GYLSG+SRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  DEPSLLLGSPNGYLSGDSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 165 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 224
           YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 225 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 284
           GCPQRIARRGNYGAFLMDNLPLVKAIV+K++SNLQVPVSCKIR+FPNLQDT+NYARMLED
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVQKLSSNLQVPVSCKIRIFPNLQDTLNYARMLED 240

Query: 285 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 344
           AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA++IPVLANGNIRHMEDV+NCLQETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVQIPVLANGNIRHMEDVNNCLQETGV 300

Query: 345 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 404
           EGVLSAETLLENPALFAGFRTA+W  GC+E+ RDGNLDQA LLV+YLKLCE+YPVPWR+I
Sbjct: 301 EGVLSAETLLENPALFAGFRTAEWVEGCDENCRDGNLDQAALLVEYLKLCEQYPVPWRII 360

Query: 405 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAIS 464
           RSHVHK+LGDWFKIHPH+REDFNAQ KLTFEFLY MV+RLRELQVKIPLY+KDSPAS IS
Sbjct: 361 RSHVHKMLGDWFKIHPHVREDFNAQFKLTFEFLYIMVDRLRELQVKIPLYLKDSPASEIS 420

BLAST of Cp4.1LG16g01540 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 612.1 bits (1577), Expect = 3.8e-175
Identity = 294/403 (72.95%), Postives = 338/403 (83.87%), Query Frame = 0

Query: 59  LSLRKSFMASSRAQTDLP-------QDLDDLLCSDPHTQRQDECSSLTKSNDSTLLLGSP 118
           L  RKS ++ +RA T  P       Q LDD+LCS+    + +E      ++     LGSP
Sbjct: 11  LRTRKSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEETVDTAPAS-----LGSP 70

Query: 119 SGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIF 178
           S  LS ++R+ERAWAHW KLGRPK IVAPMVDNSELPFRLLC+KYGA+AAYTPMLHSRIF
Sbjct: 71  SRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIF 130

Query: 179 TENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRG 238
           TE EKYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCPQRIARRG
Sbjct: 131 TETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRG 190

Query: 239 NYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHG 298
           NYGAFLMDNLPLVK++VEK+A NL VPVSCKIR+FPNL+DT+ YA+MLEDAGCSLLAVHG
Sbjct: 191 NYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHG 250

Query: 299 RTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLL 358
           RTRDEKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV NC++ETGVEGVLSAETLL
Sbjct: 251 RTRDEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLL 310

Query: 359 ENPALFAGFRTAQWTAGCEESS-RDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLG 418
           ENPA FAGFRTA+W    EE    DG LDQ DL+V+YLKLCEK+PVPWRMIRSHVHK+LG
Sbjct: 311 ENPAAFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVHKMLG 370

Query: 419 DWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIK 454
           +WF+IHP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 371 EWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

BLAST of Cp4.1LG16g01540 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.7e-29
Identity = 79/247 (31.98%), Postives = 121/247 (48.99%), Query Frame = 0

Query: 135 KLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNME------------- 194
           KL++APMV    L FR+L  +YGA+  Y   +      + E+  N+              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 195 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLM 254
             F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L+
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALL 125

Query: 255 DNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 314
               L+  I+  +  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   D
Sbjct: 126 SKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIAD 185

Query: 315 GKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALFA 366
             +  A W  I+ V  AL IPV+ANG++   +D       TG   V+ A   + N ++F+
Sbjct: 186 RPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFS 245

BLAST of Cp4.1LG16g01540 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.3 bits (277), Expect = 2.1e-24
Identity = 64/188 (34.04%), Postives = 98/188 (52.13%), Query Frame = 0

Query: 179 NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 238
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 239 MDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 298
           +    L+  I+  +  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 299 DGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGVEGVLSAETLLENPALF 358
           D  +  A W  I+ V  AL IPV+ANG++   +D       TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 359 AGFRTAQW 366
           +    + W
Sbjct: 206 SPKGKSHW 211

BLAST of Cp4.1LG16g01540 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 1.8e-15
Identity = 52/200 (26.00%), Postives = 93/200 (46.50%), Query Frame = 0

Query: 133 RPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 192
           R KL +AP+     LPFR LC+  GA+     M       + +            +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 193 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK 252
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 253 VASNLQVPVSCKIRV--FPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSA 312
            +S ++ P++ K+R   F       +    + + G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 313 I-SAVKNA-LRIPVLANGNI 327
           I    KNA   + V+ NG++
Sbjct: 517 IYQCTKNATTNLQVIGNGDV 534

BLAST of Cp4.1LG16g01540 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.1e-09
Identity = 39/135 (28.89%), Postives = 66/135 (48.89%), Query Frame = 0

Query: 134 PKLIVAPMVDNSELPFRLLCRKYGAEA-AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 193
           P   VAPM+D ++  +R L R     A  YT M+ +      +   +       +  P+ 
Sbjct: 52  PSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIV 111

Query: 194 VQFCANDPDVLLEAARRVEPY-CDYVDLNLGCPQ-RIARRGNYGAFLMDNLPLVKAIVEK 253
           +Q   ++ + L +AA+  + Y  D ++LN GCP  ++A  G +G  LM    LV   +  
Sbjct: 112 LQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSA 171

Query: 254 VASNLQVPVSCKIRV 266
           +A+N  VPV+ K R+
Sbjct: 172 IAANTNVPVTVKCRI 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8K5824.9e-7947.99tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101... [more]
Q8C2P38.4e-7947.65tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN... [more]
Q6P1R43.2e-7847.65tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=... [more]
Q9HGN66.0e-6943.71tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st... [more]
P537592.0e-6440.95tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
XP_023512932.11.82e-31299.76tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo... [more]
XP_022944464.12.38e-30898.57tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
XP_022986543.12.38e-30898.57tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima][more]
KAG7011024.14.79e-30898.57tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosp... [more]
KAG6571229.13.74e-29398.75tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein, partial [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A6J1JGU31.15e-30898.57tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661... [more]
A0A6J1FWR01.15e-30898.57tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36... [more]
A0A1S3CHU65.47e-28191.27tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=... [more]
A0A0A0LTG24.09e-27690.57Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G428390 PE=4 SV... [more]
A0A6J1D4R68.58e-27586.76tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT5G67220.13.8e-17572.95FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.11.7e-2931.98Aldolase-type TIM barrel family protein [more]
AT3G49640.22.1e-2434.04Aldolase-type TIM barrel family protein [more]
AT4G38890.11.8e-1526.00FMN-linked oxidoreductases superfamily protein [more]
AT3G63510.11.1e-0928.89FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 133..367
e-value: 6.8E-80
score: 269.8
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 137..428
e-value: 3.0E-73
score: 246.6
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 135..365
e-value: 1.3573E-105
score: 311.351
NoneNo IPR availablePANTHERPTHR11082TRNA-DIHYDROURIDINE SYNTHASEcoord: 115..453
NoneNo IPR availablePANTHERPTHR11082:SF35BNAA09G07510D PROTEINcoord: 115..453
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 134..410
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 217..235

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g01540.1Cp4.1LG16g01540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity