Cp4.1LG16g01440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG16g01440
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cytochrome P450 81D1-like
Location	Cp4.1LG16: 3352024 .. 3352996 (+)
RNA-Seq Expression	Cp4.1LG16g01440
Synteny	Cp4.1LG16g01440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGGTTACAAAAACAATCAATTTTTACTTTTAAATTTACTAGCAAATTGTTAATAATACATTACGAGATGAAATAAACCGTAACACACATAACTGTCGTAATAAAATTCATCAAGCTAAGATTTCCCAAAAACTTTCGCGTATTTGTATTAATGGAAATTAAATGGAAACTCAATTTTCTCCTCTTCATTTACTCTATATATTCATAGTTGAAATGAGATGATAACCAACAAAGCAAAGAAAAAAAACATGGAAAACCTTATGCTTTGTCTCTGTCTCCCTCTAATGGCATTCATATCAGCTCTCATATTTCTACTCCGACCCTCCACAAAGCTTCCCCCAAGTCCCCCTTTTTCCCTCCCAATTATAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAGTTATGCAAAAATGTGGGAGTTTTGATTCAAGAAGTTTTATTCCATTGTTGAATTGGATAGATATTACTCATTATGAGAAGAGGATAA

mRNA sequence

ATGTTAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAATATTACTCATTATGAGAAGAGGATAA

Coding sequence (CDS)

ATGTTAGGCCATCTCTATCTCCTAAAGCGTCCCGTCCATCAAACCCTTCAAAAGCTTTCTAACAAGTATGGCCATGTCTTCACTCTCCGCTTAGGCTCTCAACTCGTTGTCGTTGTCTCGTCTCCTTCGGTGGTTAAAGAATGCTTCACAATGAATGATATCATTCTAGCAAATCGACCAAGCTCGAACATTATCAAGCACTTGACTTACAACAACACGATCTTTTCGGCGTCCCCATATGGCGATCATTGGCGCGAACTCCGCCGAATTAGCACCATAGAGATATTCTCAGCCACTCGCCTTAATATGTTCTTGGAGACAAGGAAGGACGAAATCAAACACCTACTTGGTAAATTGTGTTGCATTTCAAGTGATAGCTTTGTGAGAGTTGAGATGAGTTCCATGATTTCTGAGTTGTCGTCAAATATTGTGATGAGAATGGTGATGGGGAAGAGGTTTTATGGAGAGGGTGTGTCGGATTTGGAACAAGCGAGGAAATTTAGAGATATTGTGAAGCAAATATTACTCATTATGAGAAGAGGATAA

Protein sequence

MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILLIMRRG

Homology

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BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 5.7e-47
Identity = 96/177 (54.24%), Postives = 128/177 (72.32%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LK+P+H T   LS KYG +F+L  GS+LVVVVSS ++ +ECFT NDI+LANRP
Sbjct: 41  IIGNLHQLKQPLHHTFHTLSQKYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
                K++ YNNT  + SPYGDHWR LRRI +IEI S+ RLN FLE R+DEI  L+ KL 
Sbjct: 101 HFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLA 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 176
             S + F  VE+  M SE++ N +MRMV GKR+YG    VSD+E+AR FR I+K+++
Sbjct: 161 QKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVV 217

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.7e-43
Identity = 88/174 (50.57%), Postives = 126/174 (72.41%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHL+L+ + +H++L  LS KYG VF+L+LG++LV+VVSSP+  +ECFT NDI+ ANRP
Sbjct: 42  VIGHLHLIGKLLHRSLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRP 101

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
              + K++ YN T    SPYG+HWR LRR++ +EIFSA  LN FL  R+DE+K LL  L 
Sbjct: 102 LFILGKYIGYNYTTMVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLY 161

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
             S   F +VEM S +SELS N+ MRMV GKR++G+ V D ++A+ FR ++ ++
Sbjct: 162 QSSGQDFGKVEMKSKLSELSFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEV 214

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 1.2e-41
Identity = 84/177 (47.46%), Postives = 127/177 (71.75%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LKRP+H+T + LS KYGHVF+L  GS+LVVVVSS S  ++CFT ND++LANRP
Sbjct: 41  IIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
                K++ YN T   ++ YG+HWR LRRI+ +++ S  R+N F   R+DE + L+ +L 
Sbjct: 101 RFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLA 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 176
             SS +F  +E+SS + +++ N +MRM+ GKR+YGE    SDL++A +FRD+V ++L
Sbjct: 161 DDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELL 217

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 2.7e-41
Identity = 86/183 (46.99%), Postives = 126/183 (68.85%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++G+L+ LK P+H+T   LS  YG +F+L  GS+LVVVVSSPS+  ECFT NDIILANRP
Sbjct: 39  IIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKL- 120
                K++ YN T   ++ YGDHWR LRRI+TI++ S  RLN FL  R+DE   L+ KL 
Sbjct: 99  RFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLL 158

Query: 121 --CCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGE--GVSDLEQARKFRDIVKQIL 179
                    F +VE+   ++E++ N +MRM+ GKR+YG+   VSD+E+A++FR+I+ +++
Sbjct: 159 KDVVSEGFGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMM 218

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BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.0e-40
Identity = 89/180 (49.44%), Postives = 121/180 (67.22%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLS-----NKYGHVFTLRLGSQLVVVVSSPSV-VKECFTMNDI 60
           ++GHL LLK P+H+TL+  S     N  G V +LRLGS+LV VVSS  V  +ECF  ND+
Sbjct: 43  IIGHLRLLKPPIHRTLRSFSETLDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDV 102

Query: 61  ILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKH 120
           +LANRP   I KH+ YNNT   A+PYGDHWR LRR+ TIEIFS  RLN FL  R DE++ 
Sbjct: 103 VLANRPQVIIGKHVGYNNTNMIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRR 162

Query: 121 LLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQI 175
           L+ +L  ++      VE+  M+ +L+ N +MRM+ GKR+YGE  +D E+A++ R +V  +
Sbjct: 163 LISRLSRLAGTKKTVVELKPMLMDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADV 222

[Close]

BLAST of Cp4.1LG16g01440 vs. NCBI nr
Match: XP_023513339.1 (cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 341 bits (874), Expect = 3.32e-113
Identity = 171/175 (97.71%), Postives = 175/175 (100.00%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP
Sbjct: 39  IIGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC
Sbjct: 99  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
           CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQ++
Sbjct: 159 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQVM 213

[Close]

BLAST of Cp4.1LG16g01440 vs. NCBI nr
Match: XP_022944471.1 (cytochrome P450 81D1-like [Cucurbita moschata])

HSP 1 Score: 320 bits (821), Expect = 3.48e-105
Identity = 162/175 (92.57%), Postives = 169/175 (96.57%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHLYLLKRPVHQTLQKLSNKYGHVF+LRLGSQLVVVVSSPS VKECFTMNDIILANRP
Sbjct: 39  IIGHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           SSNIIKHLTYNNTIFSAS YGDHWRELRRISTIEIFSA+RLNMFLETRKDEIK +LGKLC
Sbjct: 99  SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
            ISSDSF RVEMSSMISELSSNIVMRMVMGKRFYGEGV DLEQARKFRDIVKQ++
Sbjct: 159 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVM 213

[Close]

BLAST of Cp4.1LG16g01440 vs. NCBI nr
Match: XP_038902550.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 289 bits (740), Expect = 5.51e-93
Identity = 144/175 (82.29%), Postives = 156/175 (89.14%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHLYLLKRP+HQTLQKLS KYGHVF+LRLGSQLVVVVSSPS VKECFT+NDI LANRP
Sbjct: 39  IIGHLYLLKRPIHQTLQKLSKKYGHVFSLRLGSQLVVVVSSPSAVKECFTVNDITLANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
            +NI KHLTYNNTIFSAS YGDHWR+LRRISTIEIFS TRLNMFLE RKDEIK LLGKLC
Sbjct: 99  QTNITKHLTYNNTIFSASSYGDHWRDLRRISTIEIFSTTRLNMFLEIRKDEIKRLLGKLC 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
            ISSD F  VEM SM S+LSSNIVMRMV GKRFYGE V D EQA++FRDIVKQ++
Sbjct: 159 GISSDDFASVEMGSMFSKLSSNIVMRMVTGKRFYGEDVLDAEQAKEFRDIVKQVM 213

[Close]

BLAST of Cp4.1LG16g01440 vs. NCBI nr
Match: KAG7011031.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 238 bits (607), Expect = 8.21e-78
Identity = 122/131 (93.13%), Postives = 125/131 (95.42%), Query Frame = 0


Query: 51  MNDIILANRPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKD 110
           MNDIILANRPSSNIIKHLTYNN IFSAS YGDHWRELRRISTIEIFSA+RLNMFLETRKD
Sbjct: 1   MNDIILANRPSSNIIKHLTYNNMIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKD 60

Query: 111 EIKHLLGKLCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDI 170
           EIK +LGKLC ISSDSF RVEMSSMISELSSNIVMRMVMGKRFYGEGV DLEQARKFRDI
Sbjct: 61  EIKRVLGKLCRISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDI 120

Query: 171 VKQILLIMRRG 181
           VKQILL+MRRG
Sbjct: 121 VKQILLVMRRG 131

[Close]

BLAST of Cp4.1LG16g01440 vs. NCBI nr
Match: KAF3958279.1 (hypothetical protein CMV_016797 [Castanea mollissima])

HSP 1 Score: 226 bits (575), Expect = 1.53e-70
Identity = 105/175 (60.00%), Postives = 140/175 (80.00%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+L+K+P+H+T  +LS KYGHVF+LR GS+LVV++SSPS V+ECFT NDI+LANRP
Sbjct: 41  ILGHLHLVKKPLHRTFHRLSQKYGHVFSLRFGSRLVVIISSPSAVEECFTKNDIVLANRP 100

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
             ++ +HL YN T   ++PYGDHWR LRRIST+EIFS  RLNMF+  R+DE+K LL KL 
Sbjct: 101 GLSVGRHLGYNYTTLVSAPYGDHWRNLRRISTLEIFSTNRLNMFVGIRRDEVKCLLRKLS 160

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
             S   F +VE+ SM SEL+ NI+MRMV GKR+YGE V D+E+AR+FR++V +++
Sbjct: 161 KNSCQGFAKVELKSMFSELTFNIIMRMVAGKRYYGEDVKDVEEAREFRELVSEVI 215

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL
Match: A0A6J1FUI0 (cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=1)

HSP 1 Score: 320 bits (821), Expect = 1.68e-105
Identity = 162/175 (92.57%), Postives = 169/175 (96.57%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           ++GHLYLLKRPVHQTLQKLSNKYGHVF+LRLGSQLVVVVSSPS VKECFTMNDIILANRP
Sbjct: 39  IIGHLYLLKRPVHQTLQKLSNKYGHVFSLRLGSQLVVVVSSPSAVKECFTMNDIILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
           SSNIIKHLTYNNTIFSAS YGDHWRELRRISTIEIFSA+RLNMFLETRKDEIK +LGKLC
Sbjct: 99  SSNIIKHLTYNNTIFSASSYGDHWRELRRISTIEIFSASRLNMFLETRKDEIKRVLGKLC 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
            ISSDSF RVEMSSMISELSSNIVMRMVMGKRFYGEGV DLEQARKFRDIVKQ++
Sbjct: 159 RISSDSFARVEMSSMISELSSNIVMRMVMGKRFYGEGVLDLEQARKFRDIVKQVM 213

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL
Match: A0A2N9GUU6 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34038 PE=3 SV=1)

HSP 1 Score: 227 bits (578), Expect = 3.09e-72
Identity = 108/179 (60.34%), Postives = 139/179 (77.65%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+L+K+P+H+T  +LS KYG VF+++ GS+LVV+VSSPS V+ECFT NDI+LANRP
Sbjct: 42  ILGHLHLIKKPIHRTFHRLSQKYGQVFSVKFGSRLVVIVSSPSAVEECFTKNDIVLANRP 101

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
            + + +HL YN+T  + SPYGDHWR LRRI  +EIFS  RLNMFL  RKDE+KHLL KL 
Sbjct: 102 RNLVGEHLHYNHTTMATSPYGDHWRNLRRIGALEIFSTNRLNMFLGIRKDEVKHLLWKLS 161

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILLIMR 179
             S   F +VE+ SM SEL+ NI+MRMV GKR+YGE + D E+ARKFR +VK+I  + R
Sbjct: 162 RNSCQGFAKVELKSMFSELTFNIIMRMVAGKRYYGEDLKDEEEARKFRRLVKEIASVWR 220

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL
Match: A0A2N9HSY0 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS43010 PE=3 SV=1)

HSP 1 Score: 228 bits (582), Expect = 6.32e-71
Identity = 112/177 (63.28%), Postives = 138/177 (77.97%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+L+K+P+H+T  +LS KYG VF+++ GSQLVV+VSSPS V+ECFT ND+ILANRP
Sbjct: 39  ILGHLHLIKKPIHRTFHRLSQKYGKVFSIKFGSQLVVIVSSPSAVEECFTKNDVILANRP 98

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
            S I KHL YN T   ASPYGDHWR LRRIS +EIFS  RLNMFL  R+DE+KHLL KL 
Sbjct: 99  RSLIGKHLAYNFTTLIASPYGDHWRNLRRISALEIFSNNRLNMFLGIRRDEVKHLLWKLS 158

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQILLI 177
             S   F +VE+ SM SEL+ NI+MRMV GKR+YGE V   E+ARKFR +VK+I+ +
Sbjct: 159 RNSCQGFAKVELKSMFSELTFNIIMRMVAGKRYYGEDVKYEEEARKFRGLVKEIVAL 215

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL
Match: A0A7N2R7H8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 230 bits (586), Expect = 3.95e-70
Identity = 109/175 (62.29%), Postives = 141/175 (80.57%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+LLK+P+H+T  +LS KYGHVF+LR GS+LVV++SSPS V+ECFT NDI+LANRP
Sbjct: 42  ILGHLHLLKKPLHRTFHRLSQKYGHVFSLRFGSKLVVIISSPSAVEECFTKNDIVLANRP 101

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
             ++ +HL YN T   ++PYGDHWR LRRIS +EIFS  RLNMFL  R+DEIKHLL KL 
Sbjct: 102 RFSVGRHLGYNYTTMVSAPYGDHWRNLRRISALEIFSTNRLNMFLGIRRDEIKHLLHKLS 161

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
             S+  F +VE+ SM SEL+ NI+MRMV GKR+YGE V D+E+AR+FR+I+ +I+
Sbjct: 162 NNSTQGFAKVELKSMFSELTFNIIMRMVGGKRYYGEDVKDVEEARQFREIMNEIV 216

[Close]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL
Match: A0A2N9HRF0 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42212 PE=3 SV=1)

HSP 1 Score: 223 bits (567), Expect = 1.44e-69
Identity = 107/175 (61.14%), Postives = 138/175 (78.86%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGHVFTLRLGSQLVVVVSSPSVVKECFTMNDIILANRP 60
           +LGHL+L+K+P+H+T  +LS KYG VF+++ GS+LVV+VSSPS V+ECFT NDIILANRP
Sbjct: 45  ILGHLHLIKKPIHRTFHRLSQKYGQVFSVKFGSRLVVIVSSPSAVEECFTKNDIILANRP 104

Query: 61  SSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKLC 120
            + + KHL+YN+T   ASPYGDHWR LRRI+ +EIFS  RLNM L  RKDE+KHLL KL 
Sbjct: 105 RNLLGKHLSYNHTTVVASPYGDHWRNLRRIAALEIFSTNRLNMCLGIRKDEVKHLLWKLS 164

Query: 121 CISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 175
             S   F +VE+ SM  EL+ NI+MRMV GKR+YGE + D E+ARKFR ++K+I+
Sbjct: 165 RNSCQGFAKVELKSMFLELTFNIIMRMVAGKRYYGEDLKDEEEARKFRRLMKEIV 219

[Close]

BLAST of Cp4.1LG16g01440 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 182.2 bits (461), Expect = 3.8e-46
Identity = 91/176 (51.70%), Postives = 123/176 (69.89%), Query Frame = 0


Query: 2   LGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILANR 61
           +GHL+LLK+P+H+T    S   G   +F+LRLG+ L VVVSS S+ +ECFT NDI+LANR
Sbjct: 86  IGHLHLLKQPLHRTFLSFSQSLGDAPIFSLRLGNHLTVVVSSYSIAEECFTKNDIVLANR 145

Query: 62  PSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGKL 121
           P   + KH+ YN T  +++PYGDHWR LRRI T+EIFS+ +LN FL  RKDEI+HLL +L
Sbjct: 146 PKFILGKHIEYNFTTMTSAPYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRL 205

Query: 122 CCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
              S   F +VEM  +  +L+ N ++RMV GKRFYGEG    E AR+   ++ +I+
Sbjct: 206 SKNSQHGFAKVEMRQLFYDLTINNILRMVAGKRFYGEGTEQDEVARRVTQLIDEIV 261

[Close]

BLAST of Cp4.1LG16g01440 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-44
Identity = 90/177 (50.85%), Postives = 124/177 (70.06%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYG--HVFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK P+H+T    S   G   +F LRLG++L VVVSS S+ +ECFT NDI+ AN
Sbjct: 56  LIGHLHLLKLPLHRTFLSFSQSLGGAPIFCLRLGNRLTVVVSSYSIAEECFTKNDIVFAN 115

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   + KH+ YN+T  +++PYGDHWR LRRI+T+EIF++ +LN FL  RKDEI+ LL +
Sbjct: 116 RPELILGKHIEYNSTTMTSAPYGDHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLR 175

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L   S   F +VEM  ++ EL+ N V RMV GKRFYGEG    E A++ R ++ +I+
Sbjct: 176 LSKNSRHGFAKVEMRHLLFELTINNVFRMVAGKRFYGEGTEQDEVAQQVRHLMDEIV 232

[Close]

BLAST of Cp4.1LG16g01440 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-44
Identity = 87/177 (49.15%), Postives = 126/177 (71.19%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL LLK P+H+T   LS    +  +F+LRLG++LV V SS S+ +ECFT ND++LAN
Sbjct: 40  VIGHLRLLKPPIHRTFLSLSQSLNNAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLAN 99

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP+  + KH+ Y+ T   A+ YGDHWR LRRI ++EIFS  RLN FL  RKDEI+ L+ +
Sbjct: 100 RPNFILAKHVAYDYTTMIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFR 159

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L    S  FV+V+M SM+S+L+ N ++RMV GKR+YG+GV D  +A++ R ++  ++
Sbjct: 160 LSRNFSQEFVKVDMKSMLSDLTFNNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVV 216

[Close]

BLAST of Cp4.1LG16g01440 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 171.0 bits (432), Expect = 8.7e-43
Identity = 83/177 (46.89%), Postives = 124/177 (70.06%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK+PVH+T   +S   G+  +F LRLG++LV V+SS S+ +ECFT ND++LAN
Sbjct: 41  VIGHLHLLKQPVHRTFHSISKSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLAN 100

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   + KH+ YN T   A+ YGDHWR LRRI+ +EIFS+ R++ F   RKDEI+ L+  
Sbjct: 101 RPDIIMAKHVGYNFTNMIAASYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITH 160

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQIL 176
           L   S   FV VE+ S+++ L+ N ++ MV GKR+YG G  D ++A+  R+++ +I+
Sbjct: 161 LSRDSLHGFVEVELKSLLTNLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIM 217

[Close]

BLAST of Cp4.1LG16g01440 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 169.1 bits (427), Expect = 3.3e-42
Identity = 87/175 (49.71%), Postives = 121/175 (69.14%), Query Frame = 0


Query: 1   MLGHLYLLKRPVHQTLQKLSNKYGH--VFTLRLGSQLVVVVSSPSVVKECFTMNDIILAN 60
           ++GHL+LLK P+H+T   LS   G+  VF LRLG++LV V+SS S+ +ECFT ND++LAN
Sbjct: 41  VIGHLHLLKPPLHRTFLSLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLAN 100

Query: 61  RPSSNIIKHLTYNNTIFSASPYGDHWRELRRISTIEIFSATRLNMFLETRKDEIKHLLGK 120
           RP   I KHL YN T   ++ YGDHWR LRRI+ +EIFS  RLN FL  RKDEI+ L+  
Sbjct: 101 RPKFTISKHLGYNATYLLSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISH 160

Query: 121 LCCISSDSFVRVEMSSMISELSSNIVMRMVMGKRFYGEGVSDLEQARKFRDIVKQ 174
           L   S   FV VEM ++++ L+SN  +RM+ GKR++GE   D + A+  +++V +
Sbjct: 161 LSRDSLHGFVEVEMKTLLTNLASNTTIRMLAGKRYFGE---DNDDAKLVKNLVSE 212

The following BLAST results are available for this feature:

BLAST of Cp4.1LG16g01440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WNQ8	5.7e-47	54.24	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]
W8JMU7	1.7e-43	50.57	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
P93147	1.2e-41	47.46	Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2	[more]
Q6WNQ9	2.7e-41	46.99	Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P...	[more]
Q9FG65	1.0e-40	49.44	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]

BLAST of Cp4.1LG16g01440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023513339.1	3.32e-113	97.71	cytochrome P450 81D1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944471.1	3.48e-105	92.57	cytochrome P450 81D1-like [Cucurbita moschata]	[more]
XP_038902550.1	5.51e-93	82.29	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7011031.1	8.21e-78	93.13	Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAF3958279.1	1.53e-70	60.00	hypothetical protein CMV_016797 [Castanea mollissima]	[more]

BLAST of Cp4.1LG16g01440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUI0	1.68e-105	92.57	cytochrome P450 81D1-like OS=Cucurbita moschata OX=3662 GN=LOC111448921 PE=3 SV=...	[more]
A0A2N9GUU6	3.09e-72	60.34	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34038 PE=3 SV=1	[more]
A0A2N9HSY0	6.32e-71	63.28	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS43010 PE=3 SV=1	[more]
A0A7N2R7H8	3.95e-70	62.29	Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A2N9HRF0	1.44e-69	61.14	Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42212 PE=3 SV=1	[more]

BLAST of Cp4.1LG16g01440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23190.1	3.8e-46	51.70	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]
AT2G23220.1	1.6e-44	50.85	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT4G37370.1	1.6e-44	49.15	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]
AT4G37320.1	8.7e-43	46.89	cytochrome P450, family 81, subfamily D, polypeptide 5	[more]
AT4G37330.1	3.3e-42	49.71	cytochrome P450, family 81, subfamily D, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 44..65 score: 19.98 coord: 20..39 score: 45.5 coord: 139..157 score: 24.38
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..166 e-value: 8.5E-19 score: 67.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..181 e-value: 1.4E-30 score: 108.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..177
None	No IPR available	PANTHER	PTHR24298:SF840	P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 8, PUTATIVE-RELATED	coord: 1..175
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 1..175

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG16g01440.1	Cp4.1LG16g01440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen