Cp4.1LG16g01330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG16g01330 vs. ExPASy Swiss-Prot
Match: Q5XV31 (FRIGIDA-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=FRL5 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 6.8e-19
Identity = 77/270 (28.52%), Postives = 129/270 (47.78%), Query Frame = 0

Query: 793  LEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWS---TLLLKQLKQISPSIGPKER 852
            L+ + DPAKL LD             + G+E   L  S   +LLL QLK++ P IG   +
Sbjct: 572  LKCTPDPAKLFLDTSMALCP---TNTEGGYEFKMLITSASCSLLLNQLKKLLPKIGHPVK 631

Query: 853  EDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASE 912
             DA KLA+  K  +       ++ + FL  +  +G+ + F  D++L L +N      + +
Sbjct: 632  GDAKKLAVYWKDKIAKSKRDQLEVICFLQFLGIFGIVSEFKADDLLGLLDNSYWQTVSPD 691

Query: 913  LCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLA-SFRPVQ-ILNEYLRDARNATVK 972
            LC   G +  I   +QNLI T   ++A+ +I  + +   F+PV  I+N+ LR  + +  K
Sbjct: 692  LCQFLGLDDAIPGFIQNLIKTGHRIKAIDYIYSFGMVHRFQPVSAIINDSLRITKESAEK 751

Query: 973  AINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKR 1032
            +  +     ++     V AID+++ AL + + C++   L SE     LE  + SL  ++R
Sbjct: 752  SYRE----AKNESTTQVAAIDRQVRALRAAIKCISCHKLESEFQLGDLEEQIKSLLKLRR 811

Query: 1033 LIPNSHGQPISLTDQQPHSIVVQPQS--PP 1056
               N  G        +P S + Q Q+  PP
Sbjct: 812  NTSNGSGS--GSASSKPDSTIKQSQTAKPP 832

HSP 2 Score: 55.8 bits (133), Expect = 3.9e-06
Identity = 167/757 (22.06%), Postives = 319/757 (42.14%), Query Frame = 0

Query: 18  MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 77
           MEK+ S +++ +  + +  K  E L   A S LLL++QWK++E +FDSTR +++   +EL
Sbjct: 1   MEKVTSGLELVDISKRNFRKTLESLQEGAHSLLLLTIQWKEIESYFDSTRSVLEERAKEL 60

Query: 78  ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 137
           E  E++IK+K   LE  EK +    + ++ K++E FE        +    ++  + ++ Q
Sbjct: 61  EALEESIKVKALELEKKEKELCLIDESMKAKQSE-FEKKEKDFDLEQKAEVEKRKREVEQ 120

Query: 138 -ERLGGLLKDIE-LKEDTVNKVCMRILDVKKEFDDKEKAFDMI---RKRIDDCEYVMELK 197
            E+    ++ +E + ++ + ++ +R  +++ + ++ EK  + I    K   + E ++ L 
Sbjct: 121 LEKFTTRMESVERVSDEKLMELGLRATELELKMEEVEKHRERIVAGDKLRGEFEPLVSLL 180

Query: 198 EQKLNGILQLIEERSIECDFKGKSVEMIK--ALLQEHEKELATKKKQYDAIQMAIKERDV 257
            + + G+   +  +         + EM+K    L      L   K   DAI+ + KE   
Sbjct: 181 AKNM-GLSVTMPVKCSTLYLNENADEMVKKNTALARMVPYLDPAKVVLDAIEGSFKE--- 240

Query: 258 ELKLKEKELQTIQNMIATKW--KEKRLDKMEKAI--KLRTEELELKEKEFGVMESKLKSL 317
                +K+L    + +   W    + L KM   I  +++ E   L     G  ++ +K+ 
Sbjct: 241 ---YWKKDLGEADDRVVNSWIVLLENLIKMNLKITPQVKQEATPLGIAWLGKAKANMKND 300

Query: 318 SEELLSKESELESIKT-CIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKE 377
             ++      L +     +  H   L + E+ L      + D    +  L      +   
Sbjct: 301 PPQVFGCALFLAAYGLGSLTTHGVLLTLVERFL------LYDHAPKLFRLLGLEEKVSGA 360

Query: 378 IVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNL 437
           +    K    KE +L  L + + ++   +  P  +  + +    D   +  +   G    
Sbjct: 361 VETLKK----KEEYLATL-KFICEFRLYKLCPGGRPGELLIEFFDSSDKAARVIAGTGTS 420

Query: 438 LRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVAL--LEKGG 497
           +      R K  K +     K  +E   +    +  LK +  +K D ++Q A+  ++K  
Sbjct: 421 MEAQKARREKK-KADAAMAIKYIKEAKAETMFPAKILKRLAVVKNDESAQRAMEPVQKSY 480

Query: 498 EGR---LKEIQHKGLGEKLDSKDKDTSLVRDLME-TCNEKVRLVKKEESGCIPAASS--- 557
           E R    K ++       +  + K       L E T   +   VK+ E  C+P       
Sbjct: 481 EKRQSTTKGVEKSEAKSSIPYEQKHVIKRPRLTEPTAPSQNLTVKQPEVVCVPTGKQVKE 540

Query: 558 -------NTLNFHIG------SALDGTLLLVLLCEHLKLHDLVRTE-LIITLKTSSDPAK 617
                  +T+  H        + L G++   +L E ++   L  +E L   LK + DPAK
Sbjct: 541 SGADHQPDTIATHPSGTETKLNILSGSIKADMLRELVEKQPLKESEDLSNALKCTPDPAK 600

Query: 618 LVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWK 677
           L LD      P +T     +  +      C  L   L K  P+I  P+K +A KLA  WK
Sbjct: 601 LFLDTSMALCPTNT-EGGYEFKMLITSASCSLLLNQLKKLLPKIGHPVKGDAKKLAVYWK 660

Query: 678 AKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKS 737
            K++ +  + +EV+ FL  +  F + S+F A +L  LL++      + +L + LG+ D  
Sbjct: 661 DKIAKSKRDQLEVICFLQFLGIFGIVSEFKADDLLGLLDNSYWQTVSPDLCQFLGLDD-- 720

Query: 738 SVVKIRCQLMLAGLKLSNLSAICFSFYSEGRATPSSA 740
           ++      L+  G ++  +  I +SF    R  P SA
Sbjct: 721 AIPGFIQNLIKTGHRIKAIDYI-YSFGMVHRFQPVSA 733

BLAST of Cp4.1LG16g01330 vs. ExPASy Swiss-Prot
Match: Q9CA42 (Protein CROWDED NUCLEI 3 OS=Arabidopsis thaliana OX=3702 GN=CRWN3 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-06
Identity = 104/463 (22.46%), Postives = 225/463 (48.60%), Query Frame = 0

Query: 75  EELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELD 134
           E+L+  EK + L+E+RL +V++SI+   + +   +  + +  +++      I +  SEL 
Sbjct: 241 EDLQEWEKKLTLEEDRLSEVKRSINHREERVMENERTIEKKEKILENLQQKISVAKSELT 300

Query: 135 LAQERLGGLLKDIELKEDTVNKVCMRILDVKK----EFDDK--EKAFDMIRKRIDDCEYV 194
             +E +   L DI LKE     +  ++ D+K+    EF++   E+    I K +DD + V
Sbjct: 301 EKEESIKIKLNDISLKEKDFEAMKAKV-DIKEKELHEFEENLIEREQMEIGKLLDDQKAV 360

Query: 195 MELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKER 254
           ++ + ++    L+ +  RS++ + +GK     KA +++ + E++ K+++    + A++++
Sbjct: 361 LDSRRREFEMELEQM-RRSLDEELEGK-----KAEIEQLQVEISHKEEKLAKREAALEKK 420

Query: 255 DVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKE-FGVMESKLKSLS 314
           +  +K KEK+L     +   K KEK L   EK + +  E L L++KE    ++ +++ + 
Sbjct: 421 EEGVKKKEKDLDA--RLKTVKEKEKALKAEEKKLHMENERL-LEDKECLRKLKDEIEEIG 480

Query: 315 EELLSKES----ELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIV 374
            E   +ES    E ES++   +E  + L +Q +     +Q I   +    LL      + 
Sbjct: 481 TETTKQESRIREEHESLRITKEERVEFLRLQSE----LKQQIDKVKQEEELLLKEREELK 540

Query: 375 KEIVQCSKEWEL----------KENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCL 434
           ++  +  KEWE           ++N +    E + +   +E   + +E  +    + + L
Sbjct: 541 QDKERFEKEWEALDKKRANITREQNEVAEENEKLRNLQISEKHRLKREEMTSRDNLKREL 600

Query: 435 EGLKAQKGHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKD--EKVSTYLKEIEYLKAD 494
           +G+K QK  F    + +E + ++L  E     +R EE  ++D  E+  TY K  +    +
Sbjct: 601 DGVKMQKESFEADMEDLEMQKRNLDME----FQRQEEAGERDFNERARTYEKRSQ----E 660

Query: 495 LASQVALLEKGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLME 515
               +   +K  +  ++E+Q++ L   L+ + +  S+ + L++
Sbjct: 661 ELDNINYTKKLAQREMEEMQYEKLA--LEREREQISVRKKLLK 679

BLAST of Cp4.1LG16g01330 vs. ExPASy Swiss-Prot
Match: I0J0E7 (Nuclear matrix constituent protein 1 OS=Allium cepa OX=4679 GN=NMCP1 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-06
Identity = 117/531 (22.03%), Postives = 237/531 (44.63%), Query Frame = 0

Query: 17  DMEKIVSDMK--VAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDM--IQT 76
           D+EK + +M+  +AE K ++  K  E   +EAS            E   D+ R +     
Sbjct: 161 DLEKALREMRSEIAEVKYTAEKKMTEAFALEASI----------EEKRLDTERKLHSADA 220

Query: 77  EYEELERREKAIKLKEERLEDVEKSID-----------ACSKEIELKKNELFELNRLIVK 136
           +  E  R+   I  K E +ED E+ +            A  K+I  +K  L E  + +  
Sbjct: 221 KLAEASRKSSEINRKLEDVEDRERKVQRELNSINSERKALEKDISEQKEHLREWEKKLQD 280

Query: 137 CDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKR 196
             + +      ++  +ER+      ++ KE+ + +    I   +     KE+  D+  + 
Sbjct: 281 GQNRLLDGQRHINEREERINEAEGGLKKKEEELEEAKRSIEGTRNTLKRKEEDLDVRLRS 340

Query: 197 IDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAI 256
           +   E  +ELK + L    + + E + + D + +  E I+ LL EH   L TKK++++ +
Sbjct: 341 LVSKEKEIELKMKNLQKKEKDLHEIAEKLDHRER--EEIQKLLDEHRATLDTKKREFE-L 400

Query: 257 QMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMES 316
           ++  K + V+ +LK K     +       K+  + + EK ++ + +++++KEK+      
Sbjct: 401 ELESKRKSVDEELKSKFAAVNKAEKEVNRKQGLISEGEKELESKMDKIKIKEKDLETKSK 460

Query: 317 KLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASA 376
            LK   E L S E +L + K  I + + EL V   +L+S + ++   Q+ +        A
Sbjct: 461 ALKKWEESLKSDEKKLVAEKDQIMKDTHELKVSINELESLRDALNAEQHQI--------A 520

Query: 377 IVKEIVQCSKEWELKENHL---DPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKA 436
             +E ++ SK  E +E ++     L++ ++ Y N      M+E       + K +E L+ 
Sbjct: 521 EEREKLEISK--EEREQYIQKQSELKQEIEKYRN------MQEE------LSKGIESLRE 580

Query: 437 QKGHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIE-YLKADLASQVA 496
           ++  F    +S++E+   L+ E     +  E+L K   K    L+  E   KAD+  Q+ 
Sbjct: 581 EREKFEKEWESLDEKKITLQRETKKIHEEKEKLEKWHHKDQERLRNEEANAKADIERQLE 640

Query: 497 LLEKGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDL-METCNEKVRLVKKEE 528
            ++   E     ++H+ L  + +   +   + R+L +   + ++ + KK+E
Sbjct: 641 DIKLQKEAFENTMKHERLMAQEEVARRLADVTRELELRKHDLEMNMQKKQE 656

BLAST of Cp4.1LG16g01330 vs. ExPASy Swiss-Prot
Match: A0A166B1A6 (Nuclear matrix constituent protein 1 OS=Daucus carota subsp. sativus OX=79200 GN=NMCP1 PE=1 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 5.1e-06
Identity = 105/468 (22.44%), Postives = 216/468 (46.15%), Query Frame = 0

Query: 23  SDMKVAEWKRSSL---YKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEELER 82
           +D K+AE  R       K+HE    E++    L  +   L    ++  D I  + E+L  
Sbjct: 136 ADAKLAELSRKGSDIERKSHELEARESA----LRRERLALNAEREALTDNISRQREDLRE 195

Query: 83  REKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQER 142
            E+ ++  EERL +V + ++    + E + NE           D   + K SELD  Q++
Sbjct: 196 WERKLQEDEERLAEVRRLLN----QREERANE----------NDRLYQQKQSELDGEQKK 255

Query: 143 LGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLNGI 202
           +  ++  ++ KED ++    ++   +KE D  + + ++  K + + E  +  +EQ  + I
Sbjct: 256 IEIIMVSLKNKEDDISSRIAKLNIKEKEADAVKHSLEVKEKDLTEFEQKLNAREQ--SEI 315

Query: 203 LQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEKEL 262
            +L++E     + K +S EM          E+  +K  +   +  ++ R VE++ KE E+
Sbjct: 316 QKLLDEHKAILEVKKQSFEM----------EMDKRKNDF---ENDLQNRAVEVEKKEVEV 375

Query: 263 QTIQNMIATK-----WKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKE 322
           + ++  +A +      K ++L + E+ +  + ++L  +EK   + E+K++    +LLS +
Sbjct: 376 KHLEAKLAKREHALDQKHEKLKEKEQYLASKLQDLNEREKSMKLEENKIEDERNQLLSDK 435

Query: 323 SELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWE 382
            E+  +K  I++     + Q  +L    + ++  +   + L    S + +EI  C  + E
Sbjct: 436 QEMLCLKAEIEKDRASTEEQRLKLSEEIERLKITEEERLELARLQSELKQEIENCRHQRE 495

Query: 383 LKENHLDPL-QESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEER 442
           L     D L QE M       F    ++ D     + K L+ +  QK +F  L+ S E+R
Sbjct: 496 LLLKEEDELKQEKM------RFEKEWEDLDERRTALMKDLKDITVQKENFEKLKHSEEDR 555

Query: 443 SKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEK 482
             + K +  ++ ++  EL+       ++   +E+ KA LA + +  +K
Sbjct: 556 LNNKKLDTESYVQK--ELDALRLTKDSFAATMEHEKAVLAERTSSEKK 562

BLAST of Cp4.1LG16g01330 vs. ExPASy Swiss-Prot
Match: Q0DY81 (Nuclear matrix constituent protein 1a OS=Oryza sativa subsp. japonica OX=39947 GN=NMCP1A PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 6.6e-06
Identity = 118/570 (20.70%), Postives = 252/570 (44.21%), Query Frame = 0

Query: 17  DMEKIVSDMK--VAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEM------------H 76
           D+EK + +++  +AE K  S  K  +   +EA      SL+ K LE+             
Sbjct: 119 DLEKALREIRGEIAEVKFMSEKKITDAQSLEA------SLEEKRLEIEGKLHAADAKLAE 178

Query: 77  FDSTRDMIQTEYEELERREKAI-----------KLKEERLEDVEKSIDACSKEIELKKNE 136
            +  +     + EE+E R++ +           K  E+R++  E S+    K+++  +N 
Sbjct: 179 ANRKKSQADRDLEEVEARQRRLEKEKLYFENERKAGEDRIKRQEDSLRDWDKKLKESQNR 238

Query: 137 LFELNRLI-------VKCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDV 196
           + +L R +        + D   ++K  EL+ A++ L      +++KED +NK    +   
Sbjct: 239 ILDLQRSLNDREERANENDKLFKIKQEELEEAKKALEHTKATLKIKEDDINKRLAELHLQ 298

Query: 197 KKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALL 256
           +KE + K +  +   K+I + E  +  +E+   G+ +L+E+ +++ + K +  ++     
Sbjct: 299 EKEAESKNRKLEEREKKIAEREEKVSAREKV--GLQKLLEDHNVKLESKRRDFDL----- 358

Query: 257 QEHEKELATKKKQYDAIQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKL 316
                +L  +KK +DA+   + +++ +L  +EK++++          E++L K E+ +  
Sbjct: 359 -----QLENEKKSFDAM---LVQKEADLVQREKDVRS---------SEEKLSKKEQVLNE 418

Query: 317 RTEELELKEKEFGVMESKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDS---- 376
             ++LE  + +     + LK   E L + E +L   K  I+   K+ ++ + +L+S    
Sbjct: 419 SKKKLEEWQNDLDTKSNALKKWEESLQNDEKQLSEQKLQIENERKQAEMYKLELESLKAT 478

Query: 377 ---TQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKENHLDPLQESMDDY--SNNEF 436
               ++ I   QN + L        +    Q  KE +      + L E  +D      +F
Sbjct: 479 VVAEKEKILQEQNNLKLTEEERQEHIMLTAQLKKEIDEYRMRSNSLSEETEDLRKQRQKF 538

Query: 437 PPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDLKNE-ENNFEKRSEELNKK 496
               ++ D     +++  + L  +K +      + E+R KD ++E +  ++++ E L  K
Sbjct: 539 EEEWEQLDEKRTHLEEEAKKLNNEKKNLERWHDNEEKRLKDREDELDIKYKEQGENLALK 598

Query: 497 DEKV-----STYLKEIEYLK---ADLASQVAL--------LEKGGEGRLKEIQHK--GLG 527
           ++ +        L+  E LK   ADL   + L        +EK    + +E++ K   L 
Sbjct: 599 EKSLIDNIDHQRLENEELLKRERADLQRNLQLHRHELEMEMEKKQASKERELEEKENELN 658

BLAST of Cp4.1LG16g01330 vs. NCBI nr
Match: XP_023512897.1 (uncharacterized protein LOC111777493 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2251 bits (5832), Expect = 0.0
Identity = 1201/1228 (97.80%), Postives = 1201/1228 (97.80%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK
Sbjct: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME
Sbjct: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS
Sbjct: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK
Sbjct: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
            GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE
Sbjct: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN
Sbjct: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED
Sbjct: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL
Sbjct: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS
Sbjct: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY 1140
            EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY
Sbjct: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY 1140

Query: 1141 PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1200
            PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA
Sbjct: 1141 PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1200

Query: 1201 MPRLFGFREGGRATELGNRTTSPTRSRP 1228
            MPRLFGFREGGRATELGNRTTSPTRSRP
Sbjct: 1201 MPRLFGFREGGRATELGNRTTSPTRSRP 1201

BLAST of Cp4.1LG16g01330 vs. NCBI nr
Match: XP_023512899.1 (uncharacterized protein LOC111777493 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2214 bits (5738), Expect = 0.0
Identity = 1184/1211 (97.77%), Postives = 1184/1211 (97.77%), Query Frame = 0

Query: 18   MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 77
            MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL
Sbjct: 1    MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 60

Query: 78   ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 137
            ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ
Sbjct: 61   ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 120

Query: 138  ERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 197
            ERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN
Sbjct: 121  ERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 180

Query: 198  GILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEK 257
            GILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEK
Sbjct: 181  GILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEK 240

Query: 258  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKESEL 317
            ELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKESEL
Sbjct: 241  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKESEL 300

Query: 318  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKE 377
            ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKE
Sbjct: 301  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKE 360

Query: 378  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDL 437
            NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDL
Sbjct: 361  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDL 420

Query: 438  KNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGE 497
            KNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGE
Sbjct: 421  KNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGE 480

Query: 498  KLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLNFHIGSALDGTLLLVLLC 557
            KLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLNFHIGSALDGTLLLVLLC
Sbjct: 481  KLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLNFHIGSALDGTLLLVLLC 540

Query: 558  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSE 617
            EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSE
Sbjct: 541  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSE 600

Query: 618  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQ 677
            LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQ
Sbjct: 601  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQ 660

Query: 678  ILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSNLSAICFSFYSEGRATPS 737
            ILLNSVSQYKQALELARALGIGDKSS                           EGRATPS
Sbjct: 661  ILLNSVSQYKQALELARALGIGDKSS---------------------------EGRATPS 720

Query: 738  SAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQKLVPSAILLFLEKSS 797
            SAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQKLVPSAILLFLEKSS
Sbjct: 721  SAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQKLVPSAILLFLEKSS 780

Query: 798  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 857
            DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI
Sbjct: 781  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 840

Query: 858  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 917
            DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN
Sbjct: 841  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 900

Query: 918  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAINQDNTGQE 977
            QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAINQDNTGQE
Sbjct: 901  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAINQDNTGQE 960

Query: 978  DVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIPNSHGQPI 1037
            DVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIPNSHGQPI
Sbjct: 961  DVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIPNSHGQPI 1020

Query: 1038 SLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKSEAQKLRSNHEAWQHHSP 1097
            SLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKSEAQKLRSNHEAWQHHSP
Sbjct: 1021 SLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKSEAQKLRSNHEAWQHHSP 1080

Query: 1098 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQYPSNNSTLQQLQKKRKSI 1157
            PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQYPSNNSTLQQLQKKRKSI
Sbjct: 1081 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQYPSNNSTLQQLQKKRKSI 1140

Query: 1158 QYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSAMPRLFGFREGGRATELG 1217
            QYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSAMPRLFGFREGGRATELG
Sbjct: 1141 QYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSAMPRLFGFREGGRATELG 1184

Query: 1218 NRTTSPTRSRP 1228
            NRTTSPTRSRP
Sbjct: 1201 NRTTSPTRSRP 1184

BLAST of Cp4.1LG16g01330 vs. NCBI nr
Match: KAG7011042.1 (FRIGIDA-like protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2137 bits (5536), Expect = 0.0
Identity = 1150/1231 (93.42%), Postives = 1163/1231 (94.48%), Query Frame = 0

Query: 18   MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 77
            MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL
Sbjct: 1    MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 60

Query: 78   ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 137
            ERREKAIKLKEE+LEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLK SELDLAQ
Sbjct: 61   ERREKAIKLKEEQLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKESELDLAQ 120

Query: 138  ERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 197
            ERLGGL KDIELKEDTVNKVC RILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN
Sbjct: 121  ERLGGLSKDIELKEDTVNKVCTRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 180

Query: 198  GILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEK 257
            GILQLIEERSIECDFKGKSVEMIK LLQEHEKELATKKKQYDAIQMAIKE DVELKLKEK
Sbjct: 181  GILQLIEERSIECDFKGKSVEMIKELLQEHEKELATKKKQYDAIQMAIKESDVELKLKEK 240

Query: 258  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKESEL 317
            ELQTIQNMIATKWKEKRLDKMEKAIKLRTEE+ELKEKEFGVMESKLK LSEEL+SKESEL
Sbjct: 241  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEEIELKEKEFGVMESKLKPLSEELISKESEL 300

Query: 318  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKE 377
            ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYASAIVKEI+QCSKEWELKE
Sbjct: 301  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYASAIVKEIIQCSKEWELKE 360

Query: 378  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDL 437
            NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSK+L
Sbjct: 361  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKNL 420

Query: 438  KNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGE 497
            KNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+KGGEGRLKEIQHKGLGE
Sbjct: 421  KNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLDKGGEGRLKEIQHKGLGE 480

Query: 498  KLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLNFHIGSALDGTLLLVLLC 557
            KLDSK+KD SLVRDLMETCNEKVRLVKKEESGCIP ASSN LNFH GSALDGTLLLVLLC
Sbjct: 481  KLDSKEKDISLVRDLMETCNEKVRLVKKEESGCIPTASSNMLNFHTGSALDGTLLLVLLC 540

Query: 558  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSE 617
            EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFY PHTVSEDAKID HNAKRGCIFLSE
Sbjct: 541  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYSPHTVSEDAKIDFHNAKRGCIFLSE 600

Query: 618  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQ 677
            LLLKFSPQITAPLKEEALKLAGQWKAKMSMAV+NHVEVVAFLLLVANFQLASDFDAAELQ
Sbjct: 601  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVDNHVEVVAFLLLVANFQLASDFDAAELQ 660

Query: 678  ILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSNLSAICFSFYSEGRATPS 737
            ILLNSVSQYKQALELARALGIGDKSS                           EGRATPS
Sbjct: 661  ILLNSVSQYKQALELARALGIGDKSS---------------------------EGRATPS 720

Query: 738  SAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQKLVPSAILLFLEKSS 797
            SAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLTRQKLVPSAILLFLEKSS
Sbjct: 721  SAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLTRQKLVPSAILLFLEKSS 780

Query: 798  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 857
            DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI
Sbjct: 781  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 840

Query: 858  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 917
            DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN
Sbjct: 841  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 900

Query: 918  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAINQDNTGQE 977
            QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAI QDNTGQE
Sbjct: 901  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAIIQDNTGQE 960

Query: 978  DVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIPNSHGQPI 1037
            DV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLI NSHGQPI
Sbjct: 961  DVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIRNSHGQPI 1020

Query: 1038 SLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKSEAQKLRSNHEAWQHHSP 1097
            SLTDQQPHSIVVQPQSPPRAN EVQRTYPTKGEMKQQ+NWDKSEAQKLRSNHEAWQHHSP
Sbjct: 1021 SLTDQQPHSIVVQPQSPPRANCEVQRTYPTKGEMKQQLNWDKSEAQKLRSNHEAWQHHSP 1080

Query: 1098 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ------------------- 1157
            PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ                   
Sbjct: 1081 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQP 1140

Query: 1158 ---QYPSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYN 1217
               QYPSNNSTLQQLQKKRKSIQYKN SMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYN
Sbjct: 1141 HQQQYPSNNSTLQQLQKKRKSIQYKNHSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYN 1200

Query: 1218 SRFSAMPRLFGFREGGRATELGNRTTSPTRS 1226
            SRFSAMPRLFGFREGGRATELGNRTTSPTR+
Sbjct: 1201 SRFSAMPRLFGFREGGRATELGNRTTSPTRT 1204

BLAST of Cp4.1LG16g01330 vs. NCBI nr
Match: XP_022985591.1 (uncharacterized protein LOC111483612 isoform X1 [Cucurbita maxima])

HSP 1 Score: 2119 bits (5491), Expect = 0.0
Identity = 1136/1228 (92.51%), Postives = 1164/1228 (94.79%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREK IKLKEERLEDV+KSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKTIKLKEERLEDVQKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDL QERLG L KDIELKEDTVNKVCMRIL+V KEFDDKEKAFDMI+K
Sbjct: 121  KCDDAIRLKESELDLEQERLGDLSKDIELKEDTVNKVCMRILNVNKEFDDKEKAFDMIQK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERS+ECD KG SVE+IKALL+EHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSMECDLKGNSVELIKALLKEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNM+ATKWKEKRLDKMEKAIKLRTEELE+KEKEFGVM+
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMVATKWKEKRLDKMEKAIKLRTEELEIKEKEFGVMK 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELK+NHLDPLQ+SMDDYS+ EFPPVMKEHDSISLIVDKCLEG+KAQK
Sbjct: 361  AIVKEIIQCSKEWELKDNHLDPLQDSMDDYSD-EFPPVMKEHDSISLIVDKCLEGIKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
             HFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  AHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGE+LDSK+KD SLVRDLMETCNEKVR VKKEESGCIPAASSNTLN
Sbjct: 481  KGGEGRLKEIQHKGLGEELDSKEKDISLVRDLMETCNEKVRSVKKEESGCIPAASSNTLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPA LVLDALRWFYP HTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPATLVLDALRWFYPSHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL
Sbjct: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQ+GFEESFLRWSTLLLKQLK+I
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQMGFEESFLRWSTLLLKQLKKI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFS DEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSRDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAINQDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAINQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSI+ QPQSPPRANYEVQRTY TKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLICNSHGQPISLTDQQPHSIIAQPQSPPRANYEVQRTYLTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY 1140
            EAQKL SNHEAWQHHSPPTHQPHQQHSPPTHQP           HQQHSPPTHQPHQQQ+
Sbjct: 1081 EAQKLCSNHEAWQHHSPPTHQPHQQHSPPTHQP-----------HQQHSPPTHQPHQQQH 1140

Query: 1141 PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1200
            PSNN+TLQQL+KKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA
Sbjct: 1141 PSNNATLQQLRKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1189

Query: 1201 MPRLFGFREGGRATELGNRTTSPTRSRP 1228
            MPRLFG  EGGRATELGNRTTSPTRSRP
Sbjct: 1201 MPRLFGLHEGGRATELGNRTTSPTRSRP 1189

BLAST of Cp4.1LG16g01330 vs. NCBI nr
Match: XP_022932919.1 (uncharacterized protein LOC111439474 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2118 bits (5489), Expect = 0.0
Identity = 1167/1382 (84.44%), Postives = 1179/1382 (85.31%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            M F SFF WEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MFFYSFFGWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREKAIKLKEE+LEDVEKSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKAIKLKEEQLEDVEKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDLAQERLGGL KDIELKEDTVNKVC RILDVKKEFDDKEKAFDMIRK
Sbjct: 121  KCDDAIRLKESELDLAQERLGGLSKDIELKEDTVNKVCTRILDVKKEFDDKEKAFDMIRK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIK LLQEHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKELLQEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEE+ELKEKEFGVME
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEEIELKEKEFGVME 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLK LSEEL+SKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKPLSEELISKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK
Sbjct: 361  AIVKEIIQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
            GHFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  GHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGEKLDSK+KD SLVRDLMETCNEKVRLVKKEESGCIP ASSN LN
Sbjct: 481  KGGEGRLKEIQHKGLGEKLDSKEKDISLVRDLMETCNEKVRLVKKEESGCIPTASSNMLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFY PHTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYSPHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKID HNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAV+NHVEVVAFLL
Sbjct: 601  AKIDFHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVDNHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAI QDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAIIQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSIVVQPQSPPRAN EVQRTYPTKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLIRNSHGQPISLTDQQPHSIVVQPQSPPRANCEVQRTYPTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ-- 1140
            EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ  
Sbjct: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHS 1140

Query: 1141 ------------------------------------------------------------ 1200
                                                                        
Sbjct: 1141 PPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQ 1200

Query: 1201 ------------------------------------------------------------ 1228
                                                                        
Sbjct: 1201 PHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQH 1260

BLAST of Cp4.1LG16g01330 vs. ExPASy TrEMBL
Match: A0A6J1J8N5 (uncharacterized protein LOC111483612 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483612 PE=3 SV=1)

HSP 1 Score: 2119 bits (5491), Expect = 0.0
Identity = 1136/1228 (92.51%), Postives = 1164/1228 (94.79%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREK IKLKEERLEDV+KSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKTIKLKEERLEDVQKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDL QERLG L KDIELKEDTVNKVCMRIL+V KEFDDKEKAFDMI+K
Sbjct: 121  KCDDAIRLKESELDLEQERLGDLSKDIELKEDTVNKVCMRILNVNKEFDDKEKAFDMIQK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERS+ECD KG SVE+IKALL+EHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSMECDLKGNSVELIKALLKEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNM+ATKWKEKRLDKMEKAIKLRTEELE+KEKEFGVM+
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMVATKWKEKRLDKMEKAIKLRTEELEIKEKEFGVMK 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELK+NHLDPLQ+SMDDYS+ EFPPVMKEHDSISLIVDKCLEG+KAQK
Sbjct: 361  AIVKEIIQCSKEWELKDNHLDPLQDSMDDYSD-EFPPVMKEHDSISLIVDKCLEGIKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
             HFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  AHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGE+LDSK+KD SLVRDLMETCNEKVR VKKEESGCIPAASSNTLN
Sbjct: 481  KGGEGRLKEIQHKGLGEELDSKEKDISLVRDLMETCNEKVRSVKKEESGCIPAASSNTLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPA LVLDALRWFYP HTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPATLVLDALRWFYPSHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL
Sbjct: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQ+GFEESFLRWSTLLLKQLK+I
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQMGFEESFLRWSTLLLKQLKKI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFS DEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSRDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAINQDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAINQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSI+ QPQSPPRANYEVQRTY TKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLICNSHGQPISLTDQQPHSIIAQPQSPPRANYEVQRTYLTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY 1140
            EAQKL SNHEAWQHHSPPTHQPHQQHSPPTHQP           HQQHSPPTHQPHQQQ+
Sbjct: 1081 EAQKLCSNHEAWQHHSPPTHQPHQQHSPPTHQP-----------HQQHSPPTHQPHQQQH 1140

Query: 1141 PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1200
            PSNN+TLQQL+KKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA
Sbjct: 1141 PSNNATLQQLRKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1189

Query: 1201 MPRLFGFREGGRATELGNRTTSPTRSRP 1228
            MPRLFG  EGGRATELGNRTTSPTRSRP
Sbjct: 1201 MPRLFGLHEGGRATELGNRTTSPTRSRP 1189

BLAST of Cp4.1LG16g01330 vs. ExPASy TrEMBL
Match: A0A6J1EYC4 (uncharacterized protein LOC111439474 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439474 PE=3 SV=1)

HSP 1 Score: 2118 bits (5489), Expect = 0.0
Identity = 1167/1382 (84.44%), Postives = 1179/1382 (85.31%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            M F SFF WEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MFFYSFFGWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREKAIKLKEE+LEDVEKSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKAIKLKEEQLEDVEKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDLAQERLGGL KDIELKEDTVNKVC RILDVKKEFDDKEKAFDMIRK
Sbjct: 121  KCDDAIRLKESELDLAQERLGGLSKDIELKEDTVNKVCTRILDVKKEFDDKEKAFDMIRK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIK LLQEHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKELLQEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEE+ELKEKEFGVME
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEEIELKEKEFGVME 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLK LSEEL+SKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKPLSEELISKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK
Sbjct: 361  AIVKEIIQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
            GHFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  GHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGEKLDSK+KD SLVRDLMETCNEKVRLVKKEESGCIP ASSN LN
Sbjct: 481  KGGEGRLKEIQHKGLGEKLDSKEKDISLVRDLMETCNEKVRLVKKEESGCIPTASSNMLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFY PHTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYSPHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKID HNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAV+NHVEVVAFLL
Sbjct: 601  AKIDFHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVDNHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAI QDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAIIQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSIVVQPQSPPRAN EVQRTYPTKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLIRNSHGQPISLTDQQPHSIVVQPQSPPRANCEVQRTYPTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ-- 1140
            EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ  
Sbjct: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHS 1140

Query: 1141 ------------------------------------------------------------ 1200
                                                                        
Sbjct: 1141 PPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQ 1200

Query: 1201 ------------------------------------------------------------ 1228
                                                                        
Sbjct: 1201 PHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQH 1260

BLAST of Cp4.1LG16g01330 vs. ExPASy TrEMBL
Match: A0A6J1EY41 (uncharacterized protein LOC111439474 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439474 PE=3 SV=1)

HSP 1 Score: 2114 bits (5478), Expect = 0.0
Identity = 1167/1393 (83.78%), Postives = 1179/1393 (84.64%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            M F SFF WEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MFFYSFFGWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREKAIKLKEE+LEDVEKSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKAIKLKEEQLEDVEKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDLAQERLGGL KDIELKEDTVNKVC RILDVKKEFDDKEKAFDMIRK
Sbjct: 121  KCDDAIRLKESELDLAQERLGGLSKDIELKEDTVNKVCTRILDVKKEFDDKEKAFDMIRK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIK LLQEHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKELLQEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEE+ELKEKEFGVME
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEEIELKEKEFGVME 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLK LSEEL+SKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKPLSEELISKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK
Sbjct: 361  AIVKEIIQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
            GHFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  GHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGEKLDSK+KD SLVRDLMETCNEKVRLVKKEESGCIP ASSN LN
Sbjct: 481  KGGEGRLKEIQHKGLGEKLDSKEKDISLVRDLMETCNEKVRLVKKEESGCIPTASSNMLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFY PHTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYSPHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKID HNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAV+NHVEVVAFLL
Sbjct: 601  AKIDFHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVDNHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAI QDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAIIQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSIVVQPQSPPRAN EVQRTYPTKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLIRNSHGQPISLTDQQPHSIVVQPQSPPRANCEVQRTYPTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ-- 1140
            EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ  
Sbjct: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHS 1140

Query: 1141 ------------------------------------------------------------ 1200
                                                                        
Sbjct: 1141 PPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQ 1200

Query: 1201 ------------------------------------------------------------ 1228
                                                                        
Sbjct: 1201 PHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQH 1260

BLAST of Cp4.1LG16g01330 vs. ExPASy TrEMBL
Match: A0A6J1J5A1 (uncharacterized protein LOC111483612 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483612 PE=3 SV=1)

HSP 1 Score: 2094 bits (5426), Expect = 0.0
Identity = 1125/1228 (91.61%), Postives = 1153/1228 (93.89%), Query Frame = 0

Query: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60
            MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE
Sbjct: 1    MVFSSFFCWEDRVVFFDMEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLE 60

Query: 61   MHFDSTRDMIQTEYEELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIV 120
            MHFDSTRDMIQTEYEELERREK IKLKEERLEDV+KSIDACSKEIELKKNELFELNRLIV
Sbjct: 61   MHFDSTRDMIQTEYEELERREKTIKLKEERLEDVQKSIDACSKEIELKKNELFELNRLIV 120

Query: 121  KCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRK 180
            KCDDAIRLK SELDL QERLG L KDIELKEDTVNKVCMRIL+V KEFDDKEKAFDMI+K
Sbjct: 121  KCDDAIRLKESELDLEQERLGDLSKDIELKEDTVNKVCMRILNVNKEFDDKEKAFDMIQK 180

Query: 181  RIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDA 240
            RIDDCEYVMELKEQKLNGILQLIEERS+ECD KG SVE+IKALL+EHEKELATKKKQYDA
Sbjct: 181  RIDDCEYVMELKEQKLNGILQLIEERSMECDLKGNSVELIKALLKEHEKELATKKKQYDA 240

Query: 241  IQMAIKERDVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVME 300
            IQMAIKE DVELKLKEKELQTIQNM+ATKWKEKRLDKMEKAIKLRTEELE+KEKEFGVM+
Sbjct: 241  IQMAIKESDVELKLKEKELQTIQNMVATKWKEKRLDKMEKAIKLRTEELEIKEKEFGVMK 300

Query: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYAS 360
            SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYAS
Sbjct: 301  SKLKSLSEELLSKESELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYAS 360

Query: 361  AIVKEIVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQK 420
            AIVKEI+QCSKEWELK+NHLDPLQ+SMDDYS+ EFPPVMKEHDSISLIVDKCLEG+KAQK
Sbjct: 361  AIVKEIIQCSKEWELKDNHLDPLQDSMDDYSD-EFPPVMKEHDSISLIVDKCLEGIKAQK 420

Query: 421  GHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLE 480
             HFNLLRKSIEERSK+LKNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+
Sbjct: 421  AHFNLLRKSIEERSKNLKNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLD 480

Query: 481  KGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLN 540
            KGGEGRLKEIQHKGLGE+LDSK+KD SLVRDLMETCNEKVR VKKEESGCIPAASSNTLN
Sbjct: 481  KGGEGRLKEIQHKGLGEELDSKEKDISLVRDLMETCNEKVRSVKKEESGCIPAASSNTLN 540

Query: 541  FHIGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSED 600
            FH GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPA LVLDALRWFYP HTVSED
Sbjct: 541  FHTGSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPATLVLDALRWFYPSHTVSED 600

Query: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660
            AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL
Sbjct: 601  AKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLL 660

Query: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSN 720
            LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS                 
Sbjct: 661  LVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSS----------------- 720

Query: 721  LSAICFSFYSEGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLT 780
                      EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLT
Sbjct: 721  ----------EGRATPSSAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLT 780

Query: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQI 840
            RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQ+GFEESFLRWSTLLLKQLK+I
Sbjct: 781  RQKLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQMGFEESFLRWSTLLLKQLKKI 840

Query: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFEN 900
            SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFS DEILKLFEN
Sbjct: 841  SPSIGPKEREDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSRDEILKLFEN 900

Query: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960
            VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD
Sbjct: 901  VVLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRD 960

Query: 961  ARNATVKAINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVV 1020
            ARNATVKAINQDNTGQEDV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVV
Sbjct: 961  ARNATVKAINQDNTGQEDVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVV 1020

Query: 1021 SLKDMKRLIPNSHGQPISLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKS 1080
            SLKDMKRLI NSHGQPISLTDQQPHSI+ QPQSPPRANYEVQRTY TKGEMKQQ+NWDKS
Sbjct: 1021 SLKDMKRLICNSHGQPISLTDQQPHSIIAQPQSPPRANYEVQRTYLTKGEMKQQLNWDKS 1080

Query: 1081 EAQKLRSNHEAWQHHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQQY 1140
            EAQKL SNHEAWQHHSPPTHQPHQQHS                      PPTHQPHQQQ+
Sbjct: 1081 EAQKLCSNHEAWQHHSPPTHQPHQQHS----------------------PPTHQPHQQQH 1140

Query: 1141 PSNNSTLQQLQKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1200
            PSNN+TLQQL+KKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA
Sbjct: 1141 PSNNATLQQLRKKRKSIQYKNRSMKYPRKRPSTGPVFTSSSPRVHDKKSKFQRYNSRFSA 1178

Query: 1201 MPRLFGFREGGRATELGNRTTSPTRSRP 1228
            MPRLFG  EGGRATELGNRTTSPTRSRP
Sbjct: 1201 MPRLFGLHEGGRATELGNRTTSPTRSRP 1178

BLAST of Cp4.1LG16g01330 vs. ExPASy TrEMBL
Match: A0A6J1F3H5 (uncharacterized protein LOC111439474 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111439474 PE=3 SV=1)

HSP 1 Score: 2087 bits (5407), Expect = 0.0
Identity = 1153/1376 (83.79%), Postives = 1165/1376 (84.67%), Query Frame = 0

Query: 18   MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 77
            MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL
Sbjct: 1    MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 60

Query: 78   ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 137
            ERREKAIKLKEE+LEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLK SELDLAQ
Sbjct: 61   ERREKAIKLKEEQLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKESELDLAQ 120

Query: 138  ERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 197
            ERLGGL KDIELKEDTVNKVC RILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN
Sbjct: 121  ERLGGLSKDIELKEDTVNKVCTRILDVKKEFDDKEKAFDMIRKRIDDCEYVMELKEQKLN 180

Query: 198  GILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEK 257
            GILQLIEERSIECDFKGKSVEMIK LLQEHEKELATKKKQYDAIQMAIKE DVELKLKEK
Sbjct: 181  GILQLIEERSIECDFKGKSVEMIKELLQEHEKELATKKKQYDAIQMAIKESDVELKLKEK 240

Query: 258  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKEFGVMESKLKSLSEELLSKESEL 317
            ELQTIQNMIATKWKEKRLDKMEKAIKLRTEE+ELKEKEFGVMESKLK LSEEL+SKESEL
Sbjct: 241  ELQTIQNMIATKWKEKRLDKMEKAIKLRTEEIELKEKEFGVMESKLKPLSEELISKESEL 300

Query: 318  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKE 377
            ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAV+LLTNYASAIVKEI+QCSKEWELKE
Sbjct: 301  ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVILLTNYASAIVKEIIQCSKEWELKE 360

Query: 378  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKDL 437
            NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSK+L
Sbjct: 361  NHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKSIEERSKNL 420

Query: 438  KNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGE 497
            KNEENNFEKR EELNKKDEKVSTYLKEIEYLKADLASQVALL+KGGEGRLKEIQHKGLGE
Sbjct: 421  KNEENNFEKRLEELNKKDEKVSTYLKEIEYLKADLASQVALLDKGGEGRLKEIQHKGLGE 480

Query: 498  KLDSKDKDTSLVRDLMETCNEKVRLVKKEESGCIPAASSNTLNFHIGSALDGTLLLVLLC 557
            KLDSK+KD SLVRDLMETCNEKVRLVKKEESGCIP ASSN LNFH GSALDGTLLLVLLC
Sbjct: 481  KLDSKEKDISLVRDLMETCNEKVRLVKKEESGCIPTASSNMLNFHTGSALDGTLLLVLLC 540

Query: 558  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSE 617
            EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFY PHTVSEDAKID HNAKRGCIFLSE
Sbjct: 541  EHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYSPHTVSEDAKIDFHNAKRGCIFLSE 600

Query: 618  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQ 677
            LLLKFSPQITAPLKEEALKLAGQWKAKMSMAV+NHVEVVAFLLLVANFQLASDFDAAELQ
Sbjct: 601  LLLKFSPQITAPLKEEALKLAGQWKAKMSMAVDNHVEVVAFLLLVANFQLASDFDAAELQ 660

Query: 678  ILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSNLSAICFSFYSEGRATPS 737
            ILLNSVSQYKQALELARALGIGDKSS                           EGRATPS
Sbjct: 661  ILLNSVSQYKQALELARALGIGDKSS---------------------------EGRATPS 720

Query: 738  SAKPEQPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQKLVPSAILLFLEKSS 797
            SAKPEQPESLPAKEVELSSLKNEQLSMDP+EERLYLLLNNQLTRQKLVPSAILLFLEKSS
Sbjct: 721  SAKPEQPESLPAKEVELSSLKNEQLSMDPNEERLYLLLNNQLTRQKLVPSAILLFLEKSS 780

Query: 798  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 857
            DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI
Sbjct: 781  DPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISPSIGPKEREDAMKLAI 840

Query: 858  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 917
            DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN
Sbjct: 841  DLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASELCLMFGYN 900

Query: 918  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAINQDNTGQE 977
            QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAI QDNTGQE
Sbjct: 901  QKIQELVQNLIGTKQFVRAVRFICGYKLASFRPVQILNEYLRDARNATVKAIIQDNTGQE 960

Query: 978  DVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIPNSHGQPI 1037
            DV AA VEAIDKEIDA+NSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLI NSHGQPI
Sbjct: 961  DVRAAMVEAIDKEIDAVNSVVTCVADCNLGSEISSQGLESLVVSLKDMKRLIRNSHGQPI 1020

Query: 1038 SLTDQQPHSIVVQPQSPPRANYEVQRTYPTKGEMKQQVNWDKSEAQKLRSNHEAWQHHSP 1097
            SLTDQQPHSIVVQPQSPPRAN EVQRTYPTKGEMKQQ+NWDKSEAQKLRSNHEAWQHHSP
Sbjct: 1021 SLTDQQPHSIVVQPQSPPRANCEVQRTYPTKGEMKQQLNWDKSEAQKLRSNHEAWQHHSP 1080

Query: 1098 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ------------------- 1157
            PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQ                   
Sbjct: 1081 PTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQP 1140

Query: 1158 ------------------------------------------------------------ 1217
                                                                        
Sbjct: 1141 HQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHS 1200

Query: 1218 ------------------------------------------------------------ 1228
                                                                        
Sbjct: 1201 PPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQPHQQHSPPTHQ 1260

BLAST of Cp4.1LG16g01330 vs. TAIR 10
Match: AT5G27220.1 (Frigida-like protein )

HSP 1 Score: 186.8 bits (473), Expect = 1.0e-46
Identity = 275/1189 (23.13%), Postives = 501/1189 (42.14%), Query Frame = 0

Query: 20   KIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEE--- 79
            K+ +++++ + K +++ K  + +  +AS  L+L+LQW D E H  S  + ++  + E   
Sbjct: 5    KLENEIRLCDVKANNIRKTMDMIKSQASDVLILNLQWCDFEEHLKSASEKLELRFRELVL 64

Query: 80   -----------LERREKAIKLKEERLEDVE---------------------KSIDACSKE 139
                       LE R K ++  E  + D+E                     KS++ CS E
Sbjct: 65   KEVELQNRSFALEERAKVVEAAEAEMGDLEMKASGFRSEVEEKREELGCLRKSLEECSVE 124

Query: 140  IELKKNELFELNRLIVKCDDAIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDV 199
               K+ +L E+  L+ K    + LKG EL      L     +++ +++ + +      ++
Sbjct: 125  ERSKRGQLSEIVELLRKSQVDLDLKGEELRQMVTHLERYRVEVKEEKEHLRRTDNGRREL 184

Query: 200  KKEFDDKEKAFDMIRKRIDDCEY--------------VMELKEQKLNGIL---------- 259
            ++E + K K   ++  +I DC+                +ELKE++L+ +           
Sbjct: 185  EEEIERKTKDLTLVMNKIVDCDKRIETRSLELIKTQGEVELKEKQLDQMKIDLEKYCVDV 244

Query: 260  ---------------------------------------QLIEERSI-------ECDFKG 319
                                                   +L E RS+       E + KG
Sbjct: 245  NAEKKNLGRTQTHRRKLEEEIERKTKDLTLVMDKIAECEKLFERRSLELIKTQGEVELKG 304

Query: 320  KSVEMIKALLQEHEKELATKKKQYDAIQMAIKERDVELKLKEKELQTIQNMIATKWK--- 379
            K +E +   L+ H  E+    +  +  Q   +E   E++ K KEL  + +  A   K   
Sbjct: 305  KQLEQMDIDLERHRGEVNVVMEHLEKSQTRSRELAEEIERKRKELTAVLDKTAEYGKTIE 364

Query: 380  --EKRLDKMEKAIKLRTEELELKEKEF----------GVMESKLKSLSEELLSKESEL-- 439
              E+ L   +K + +R+ EL  K+KE             + ++LK   + + SK  EL  
Sbjct: 365  LVEEELALQQKLLDIRSSELVSKKKELDGLSLDLELVNSLNNELKETVQRIESKGKELED 424

Query: 440  ------------ESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKE 499
                        ESIK  ++EHS+EL ++E++ +   ++++     +V       +  K 
Sbjct: 425  MERLIQERSGHNESIKLLLEEHSEELAIKEERHNEIAEAVRKLSLEIV-------SKEKT 484

Query: 500  IVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNL 559
            I Q S++   K+  LD  ++ +++ +  E      E  S+     +CL+  + ++     
Sbjct: 485  IQQLSEKQHSKQTKLDSTEKCLEE-TTAELVSKENELCSVKDTYRECLQNWEIKEKEL-- 544

Query: 560  LRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEG 619
              KS +E  K +++   +F+ +  EL K  E ++ + KE+   K  +  +   +E     
Sbjct: 545  --KSFQEEVKKIQDSLKDFQSKEAELVKLKESLTEHEKELGLKKKQIHVRSEKIE----- 604

Query: 620  RLKEIQHKGLGEKLDSKDKDTSLVRDLMETCNEKVRL-VKKEESGCIPAASSNTLNF-HI 679
             LK+ +     E+LD KD+        +  C ++  L  KK  S C        ++    
Sbjct: 605  -LKDKKLDAREERLDKKDEQLKSAEQKLAKCVKEYELNAKKLASFCQQNNPDQQVDLVRD 664

Query: 680  GSALDGTLLLVLLCEHLKLHDLVRTELIITLKTSSDPAKLVLDALRWFYPPHTVSEDAKI 739
             S  D   L +LL  HLK  D +  +++  LK SSDPAKLVL+ ++  +    V+   K+
Sbjct: 665  ASVCDEKTLQLLLRGHLKKCDQLHLDVLRALKASSDPAKLVLNTIQRLHEKMAVT---KL 724

Query: 740  DLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWKAKMSMAVENHVEVVAFLLLVA 799
            D  + +RG I L E L+  SP+    ++ EA+K   +WK    +  EN VEV+ FL  ++
Sbjct: 725  DPDSVRRGSICLLECLMDMSPEPKTEVQVEAIKSVTEWKNTTLVKAENPVEVLGFLHFLS 784

Query: 800  NFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKSSVVKIRCQLMLAGLKLSNLSA 859
             F LA  FDA ++Q L ++    + A  L  ALG+   SS+  +   L L          
Sbjct: 785  AFSLAYTFDADKVQNLFDAAFLRQYAPSLCEALGV---SSLAPVNNVLSL---------- 844

Query: 860  ICFSFYSEGRATPSSAKPE-QPESLPAKEVELSSLKNEQLSMDPSEERLYLLLNNQLTRQ 919
                            KPE QP   P      S   N Q ++  S      +L +     
Sbjct: 845  --------------DDKPEQQPPEAPIINSSDSRSTNVQETIASSHLGNVDVLLDPEGST 904

Query: 920  KLVPSAILLFLEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWSTLLLKQLKQISP 979
               P+ +   L+   DPA  VL+++   +     + ++G  E  ++    LL++L ++  
Sbjct: 905  SFSPNEVFTGLQGMIDPASYVLNVVNDELLGAQQRGELGLAEPVIKTLIPLLEELPRVVK 964

Query: 980  SIGPKEREDAMKLAIDLKLNMRNDTNGS-MDAVVFLLLIVSYGLTTSFSGDEILKLFENV 1039
            S       DA+++A      M N T  S ++A  FL LIV+YGL  + S D  L+    V
Sbjct: 965  S-SKHLLSDALQVATRWSWMMGNSTQMSPLEAWGFLQLIVAYGLVHATSQDNTLRFASYV 1024

Query: 1040 VLHEQASELCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLA-SFRPVQILNEYLRD 1068
               +QA +L    G +  +  LV+ L+  + +  A+RFI  +KL  +F P+++L + +  
Sbjct: 1025 AHFKQAPKLFESLGLSYAMPNLVKKLLDERHYFMAIRFIFYFKLKFNFSPLELLKDEIIT 1084

BLAST of Cp4.1LG16g01330 vs. TAIR 10
Match: AT5G27230.1 (Frigida-like protein )

HSP 1 Score: 98.2 bits (243), Expect = 4.8e-20
Identity = 77/270 (28.52%), Postives = 129/270 (47.78%), Query Frame = 0

Query: 793  LEKSSDPAKLVLDLIKGYVHQQLNKEQIGFEESFLRWS---TLLLKQLKQISPSIGPKER 852
            L+ + DPAKL LD             + G+E   L  S   +LLL QLK++ P IG   +
Sbjct: 572  LKCTPDPAKLFLDTSMALCP---TNTEGGYEFKMLITSASCSLLLNQLKKLLPKIGHPVK 631

Query: 853  EDAMKLAIDLKLNMRNDTNGSMDAVVFLLLIVSYGLTTSFSGDEILKLFENVVLHEQASE 912
             DA KLA+  K  +       ++ + FL  +  +G+ + F  D++L L +N      + +
Sbjct: 632  GDAKKLAVYWKDKIAKSKRDQLEVICFLQFLGIFGIVSEFKADDLLGLLDNSYWQTVSPD 691

Query: 913  LCLMFGYNQKIQELVQNLIGTKQFVRAVRFICGYKLA-SFRPVQ-ILNEYLRDARNATVK 972
            LC   G +  I   +QNLI T   ++A+ +I  + +   F+PV  I+N+ LR  + +  K
Sbjct: 692  LCQFLGLDDAIPGFIQNLIKTGHRIKAIDYIYSFGMVHRFQPVSAIINDSLRITKESAEK 751

Query: 973  AINQDNTGQEDVHAATVEAIDKEIDALNSVVTCVADCNLGSEISSQGLESLVVSLKDMKR 1032
            +  +     ++     V AID+++ AL + + C++   L SE     LE  + SL  ++R
Sbjct: 752  SYRE----AKNESTTQVAAIDRQVRALRAAIKCISCHKLESEFQLGDLEEQIKSLLKLRR 811

Query: 1033 LIPNSHGQPISLTDQQPHSIVVQPQS--PP 1056
               N  G        +P S + Q Q+  PP
Sbjct: 812  NTSNGSGS--GSASSKPDSTIKQSQTAKPP 832

HSP 2 Score: 55.8 bits (133), Expect = 2.8e-07
Identity = 167/757 (22.06%), Postives = 319/757 (42.14%), Query Frame = 0

Query: 18  MEKIVSDMKVAEWKRSSLYKAHERLHMEASSFLLLSLQWKDLEMHFDSTRDMIQTEYEEL 77
           MEK+ S +++ +  + +  K  E L   A S LLL++QWK++E +FDSTR +++   +EL
Sbjct: 1   MEKVTSGLELVDISKRNFRKTLESLQEGAHSLLLLTIQWKEIESYFDSTRSVLEERAKEL 60

Query: 78  ERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELDLAQ 137
           E  E++IK+K   LE  EK +    + ++ K++E FE        +    ++  + ++ Q
Sbjct: 61  EALEESIKVKALELEKKEKELCLIDESMKAKQSE-FEKKEKDFDLEQKAEVEKRKREVEQ 120

Query: 138 -ERLGGLLKDIE-LKEDTVNKVCMRILDVKKEFDDKEKAFDMI---RKRIDDCEYVMELK 197
            E+    ++ +E + ++ + ++ +R  +++ + ++ EK  + I    K   + E ++ L 
Sbjct: 121 LEKFTTRMESVERVSDEKLMELGLRATELELKMEEVEKHRERIVAGDKLRGEFEPLVSLL 180

Query: 198 EQKLNGILQLIEERSIECDFKGKSVEMIK--ALLQEHEKELATKKKQYDAIQMAIKERDV 257
            + + G+   +  +         + EM+K    L      L   K   DAI+ + KE   
Sbjct: 181 AKNM-GLSVTMPVKCSTLYLNENADEMVKKNTALARMVPYLDPAKVVLDAIEGSFKE--- 240

Query: 258 ELKLKEKELQTIQNMIATKW--KEKRLDKMEKAI--KLRTEELELKEKEFGVMESKLKSL 317
                +K+L    + +   W    + L KM   I  +++ E   L     G  ++ +K+ 
Sbjct: 241 ---YWKKDLGEADDRVVNSWIVLLENLIKMNLKITPQVKQEATPLGIAWLGKAKANMKND 300

Query: 318 SEELLSKESELESIKT-CIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIVKE 377
             ++      L +     +  H   L + E+ L      + D    +  L      +   
Sbjct: 301 PPQVFGCALFLAAYGLGSLTTHGVLLTLVERFL------LYDHAPKLFRLLGLEEKVSGA 360

Query: 378 IVQCSKEWELKENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNL 437
           +    K    KE +L  L + + ++   +  P  +  + +    D   +  +   G    
Sbjct: 361 VETLKK----KEEYLATL-KFICEFRLYKLCPGGRPGELLIEFFDSSDKAARVIAGTGTS 420

Query: 438 LRKSIEERSKDLKNEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVAL--LEKGG 497
           +      R K  K +     K  +E   +    +  LK +  +K D ++Q A+  ++K  
Sbjct: 421 MEAQKARREKK-KADAAMAIKYIKEAKAETMFPAKILKRLAVVKNDESAQRAMEPVQKSY 480

Query: 498 EGR---LKEIQHKGLGEKLDSKDKDTSLVRDLME-TCNEKVRLVKKEESGCIPAASS--- 557
           E R    K ++       +  + K       L E T   +   VK+ E  C+P       
Sbjct: 481 EKRQSTTKGVEKSEAKSSIPYEQKHVIKRPRLTEPTAPSQNLTVKQPEVVCVPTGKQVKE 540

Query: 558 -------NTLNFHIG------SALDGTLLLVLLCEHLKLHDLVRTE-LIITLKTSSDPAK 617
                  +T+  H        + L G++   +L E ++   L  +E L   LK + DPAK
Sbjct: 541 SGADHQPDTIATHPSGTETKLNILSGSIKADMLRELVEKQPLKESEDLSNALKCTPDPAK 600

Query: 618 LVLDALRWFYPPHTVSEDAKIDLHNAKRGCIFLSELLLKFSPQITAPLKEEALKLAGQWK 677
           L LD      P +T     +  +      C  L   L K  P+I  P+K +A KLA  WK
Sbjct: 601 LFLDTSMALCPTNT-EGGYEFKMLITSASCSLLLNQLKKLLPKIGHPVKGDAKKLAVYWK 660

Query: 678 AKMSMAVENHVEVVAFLLLVANFQLASDFDAAELQILLNSVSQYKQALELARALGIGDKS 737
            K++ +  + +EV+ FL  +  F + S+F A +L  LL++      + +L + LG+ D  
Sbjct: 661 DKIAKSKRDQLEVICFLQFLGIFGIVSEFKADDLLGLLDNSYWQTVSPDLCQFLGLDD-- 720

Query: 738 SVVKIRCQLMLAGLKLSNLSAICFSFYSEGRATPSSA 740
           ++      L+  G ++  +  I +SF    R  P SA
Sbjct: 721 AIPGFIQNLIKTGHRIKAIDYI-YSFGMVHRFQPVSA 733

BLAST of Cp4.1LG16g01330 vs. TAIR 10
Match: AT1G68790.1 (little nuclei3 )

HSP 1 Score: 57.4 bits (137), Expect = 9.5e-08
Identity = 104/463 (22.46%), Postives = 225/463 (48.60%), Query Frame = 0

Query: 75  EELERREKAIKLKEERLEDVEKSIDACSKEIELKKNELFELNRLIVKCDDAIRLKGSELD 134
           E+L+  EK + L+E+RL +V++SI+   + +   +  + +  +++      I +  SEL 
Sbjct: 241 EDLQEWEKKLTLEEDRLSEVKRSINHREERVMENERTIEKKEKILENLQQKISVAKSELT 300

Query: 135 LAQERLGGLLKDIELKEDTVNKVCMRILDVKK----EFDDK--EKAFDMIRKRIDDCEYV 194
             +E +   L DI LKE     +  ++ D+K+    EF++   E+    I K +DD + V
Sbjct: 301 EKEESIKIKLNDISLKEKDFEAMKAKV-DIKEKELHEFEENLIEREQMEIGKLLDDQKAV 360

Query: 195 MELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELATKKKQYDAIQMAIKER 254
           ++ + ++    L+ +  RS++ + +GK     KA +++ + E++ K+++    + A++++
Sbjct: 361 LDSRRREFEMELEQM-RRSLDEELEGK-----KAEIEQLQVEISHKEEKLAKREAALEKK 420

Query: 255 DVELKLKEKELQTIQNMIATKWKEKRLDKMEKAIKLRTEELELKEKE-FGVMESKLKSLS 314
           +  +K KEK+L     +   K KEK L   EK + +  E L L++KE    ++ +++ + 
Sbjct: 421 EEGVKKKEKDLDA--RLKTVKEKEKALKAEEKKLHMENERL-LEDKECLRKLKDEIEEIG 480

Query: 315 EELLSKES----ELESIKTCIKEHSKELDVQEKQLDSTQQSIQDCQNAVVLLTNYASAIV 374
            E   +ES    E ES++   +E  + L +Q +     +Q I   +    LL      + 
Sbjct: 481 TETTKQESRIREEHESLRITKEERVEFLRLQSE----LKQQIDKVKQEEELLLKEREELK 540

Query: 375 KEIVQCSKEWEL----------KENHLDPLQESMDDYSNNEFPPVMKEHDSISLIVDKCL 434
           ++  +  KEWE           ++N +    E + +   +E   + +E  +    + + L
Sbjct: 541 QDKERFEKEWEALDKKRANITREQNEVAEENEKLRNLQISEKHRLKREEMTSRDNLKREL 600

Query: 435 EGLKAQKGHFNLLRKSIEERSKDLKNEENNFEKRSEELNKKD--EKVSTYLKEIEYLKAD 494
           +G+K QK  F    + +E + ++L  E     +R EE  ++D  E+  TY K  +    +
Sbjct: 601 DGVKMQKESFEADMEDLEMQKRNLDME----FQRQEEAGERDFNERARTYEKRSQ----E 660

Query: 495 LASQVALLEKGGEGRLKEIQHKGLGEKLDSKDKDTSLVRDLME 515
               +   +K  +  ++E+Q++ L   L+ + +  S+ + L++
Sbjct: 661 ELDNINYTKKLAQREMEEMQYEKLA--LEREREQISVRKKLLK 679

BLAST of Cp4.1LG16g01330 vs. TAIR 10
Match: AT1G67230.1 (little nuclei1 )

HSP 1 Score: 50.1 bits (118), Expect = 1.5e-05
Identity = 127/570 (22.28%), Postives = 261/570 (45.79%), Query Frame = 0

Query: 24  DMKVAEWKRSSLYKAHERLHMEASSFLLL------SLQWKDLEMHFDSTRDMIQTE---- 83
           D ++   K S L K  E    + S  LLL      S Q++ L+  F+   + ++ E    
Sbjct: 48  DPRILPEKISELEK--ELFEYQHSMGLLLIEKKEWSSQYEALQQAFEEVNECLKQERNAH 107

Query: 84  ---YEELERRE----KAIKLKEERLEDVEKSI-DACSKEIELK---KNELFELNRLIVKC 143
                ++E+RE    KA+ ++++   D+EK++ +  ++  E+K    ++L E N L+   
Sbjct: 108 LIAIADVEKREEGLRKALGIEKQCALDLEKALKELRAENAEIKFTADSKLTEANALVRSV 167

Query: 144 DD----------AIRLKGSELDLAQERLGGLLKDIELKEDTVNKVCMRILDVKKEFDDKE 203
           ++          A+  K +E+      +    K++E +E ++ +     +  ++E D  E
Sbjct: 168 EEKSLEVEAKLRAVDAKLAEVSRKSSDVERKAKEVEARESSLQRERFSYI-AEREAD--E 227

Query: 204 KAFDMIRKRIDDCEYVMELKEQKLNGILQLIEERSIECDFKGKSVEMIKALLQEHEKELA 263
                 R+ + + E  ++  E+++     ++++R    +   K       ++++  KEL 
Sbjct: 228 ATLSKQREDLREWERKLQEGEERVAKSQMIVKQREDRANESDK-------IIKQKGKELE 287

Query: 264 TKKKQYDAIQMAIK--ERDV-----ELKLKEKELQTIQNMIATKWKE-----KRLDKMEK 323
             +K+ DA  +A+K  E DV     +L L+E+E   ++  I TK +E     ++L+  EK
Sbjct: 288 EAQKKIDAANLAVKKLEDDVSSRIKDLALREQETDVLKKSIETKARELQALQEKLEAREK 347

Query: 324 -AIKLRTEE----LELKEKEFGV-MESKLKSLSEELLSKESELESIKTCIKEHSKELDVQ 383
            A++   +E    L+  ++EF + ME K KS+ + L SK +E+E  +   K   +++  +
Sbjct: 348 MAVQQLVDEHQAKLDSTQREFELEMEQKRKSIDDSLKSKVAEVEKREAEWKHMEEKVAKR 407

Query: 384 EKQLDSTQQSIQDCQNAVVLLTNYASAIVKEIVQCSKEWELKEN----------HLDPLQ 443
           E+ LD   +  ++ +N   L     S   K +    K  E ++           +L  L 
Sbjct: 408 EQALDRKLEKHKEKENDFDLRLKGISGREKALKSEEKALETEKKKLLEDKEIILNLKALV 467

Query: 444 ESMDDYSNNEFPPVMKEHDSISLIVDKCLEGLKAQKGHFNLLRKS------IEERSKDLK 503
           E +   +  +   + KE D + +  ++  E L+ Q      + K       +++ ++DLK
Sbjct: 468 EKVSGENQAQLSEINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQELLQKEAEDLK 527

Query: 504 NEENNFEKRSEELNKKDEKVSTYLKEIEYLKADLASQVALLEKGGEGRLKEIQHKGLGEK 529
            +  +FEK  EEL+++  K+   LK I   K  L   + L E+  + + K+  ++ +  +
Sbjct: 528 AQRESFEKEWEELDERKAKIGNELKNITDQKEKLERHIHLEEERLK-KEKQAANENMERE 587