Cp4.1LG16g00190 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG16g00190
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGDSL esterase/lipase 3-like
LocationCp4.1LG16: 540744 .. 545750 (+)
RNA-Seq ExpressionCp4.1LG16g00190
SyntenyCp4.1LG16g00190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGAGTTGGTAGAAAAAAAAAAATAATGTTAAAAATCAAGCAAAAAGGACAGCTGCTTTTAGAAGGCAGAAATAATTTATAATAATTTCCTATAAGAACACAAGAACATCGGCCATTGCCATCAGTTTGAACTGAGAGTTGTAGAAATGATGTTTCCAAATCTGGGCAGCTGCTTCTTCTTCATTTTGTTTGCAGCTCTATGTGGTTTTCCCACAATCTCGAAGGCGGTCGGAATCCAACTCGGCGGTGCTCCGTCGTCGGAAAACCGCTTCGGGTTTTTCATATTTGGGGATTCCTATGTGGATGTCGGAAACAACAACTACATCAATACCACTAGTGACTTTCAGGCCAACTTCCCGCCTTACGGTGAAAGCTTCTTCCCGGTCGCCACCGGCAGGTTTACCGACGGCCGTAATATCCCAGATTTCTTAGGTGAATGTACTTACATTTTAACGTTTTCTTGTTCTTTTTTTGTCCAAGATTTGAGATGGGGTTTCAATAGGGTCGTTTAATTGCTGAAATTTGTAGAACAGTTCTGTTTCTTGTTCTTCTTCTACTTGAATCTTTGTGTTTGAAAACTGCTGGTTAATGATCTGATGAATTAGTAGCATGATTTTTTTGTTCCTTATCAAGACGAAGAGAAAGGGGTCAAAAAAATTGGGATTTTCCCGGCTCGGGGATATTGTCCTCTTTGAGCTTTCTCTCAAGGTGTTTAAAACGTGCCTGCTAGTGAGAGGTTTTCAAACCTTTGTAAAGAATGTTATGTCTACTCCCCAATCGATGTGAGATCTCACAATCCACCCCCTTCGGGGCACAACGTTCTTGCTAGCACTCGTTCCTTTCTCCAATTGATGACCACCCCCGATTCATCCCCTTTGAGACCCAACGTCCTTACTGGCACACCGCCTCGTATCCATCCCACTTTGGGGCTTAGCTTCCTTGTTGGTACATCACTTTGTGTCTAGCTCTGATACCATTTATAATGGTTCAAGCTCACCACTAGTAGATACTGTCCTCTTTGAGCTTTTCTTTTTCAGGCTTTCTCTCAAGGTTTTTAAACTCGTCTGCTAGGGAGAGGTTTTCACATCCTTGTAAAGAATGTTTTGTCTCCTTTCCAACTTATGTGGGATCTCACAATCCACCCCCTTTCGGATCCCAGCATCCTCGCTGGCACTCGCCACTTTCTCCAATGGATATGGGACCACCCCCAATCTATTCCCTTTGGGGCCCAACGTTCTTACTGGCACACTGCCTCGTATCCACCCCACTTCGGGGGTCTGCCTCCTCGCTGGCACATCGCGGTGTCTAGCTCTAATATCATTTGTAACAACCCAAGTCCATTGCTAACAGATATTGTCCTCTCTAAGTTTTCCCTTTCAGGCTTCCTTTCAAGATTTTTAAAATGCGTTTGTTAGAGAGAGGTTTCCACATCCTTATAAAAAATGCTTTGTCTCTTCTCCAATCAACGTGCGATCTCACAATCCACCCCCTTTGGGGCCCAGCATCCTCACTGGCACTCGTTCCTTTCTCCAATCTATGTGAGACCACCCCAAATCCACCCCTTTGGGGCCCAACGTCCTTGCTAACACACCTCGTATCCACCCCACTTCGAGGCTCAGCCTCCTCGTTGGCACATCGCTCAGTTTCTGGCTCTGATACCATTTATAACAGTCTAAACCCACCACTAGTAGATATTGTCCTCTTTGAGTTTTACCTTTCATGCTTCCTTTCAAAGTTTTTAATTCGCGTCTACTGGGAGAGGTTTCCACACCCTTGTAAAGAATGGTTCGTTCTCCTCTTGAACCGATGTGGGCCTCACAATTTTGGGGTAGGATTTTGCTGTTGTTGGCCACTGAAATTGATATCATTCTGAATGTTCATATTGAACTAATAACTTCAATCTTGCTGCAAACAAGGCAGAACTTGAAAGAGTACTTATTAACGCAGGTGAGTATGCAAATTTGCCTTTGCTCCCACCATATTTGGATCCCCACAATGATCTTTATGACTATGGAGTCAATTTTGCATCTGGTGGAGGTGGTGCTTTAGCTCTAAGTCATCAAGAACAGGTCCCATTCAAATCAATCAAACTAAGTTCTTTCCTCTTTCAATCAATTGAATGCATAAACATTCTTACATTGATTGATTTTCAGGCCTTAGGGCTTCAAACCCAGATGAAGTTCTTCAAAAGGGTGGACAAATCACTAAGAAAGAAGCTTGGGAATGCAAGAACTCAAAGCTTCTTCTCCAACTCTGTTTTTTTGTTTAATTTCGGAGGAAACGATTACCTAAATCCGTTCGACATAAGCTACGACATATTCAAAACCATCGACGCTCAAGAACAGTACGTGAATATGGTGATCGGAAACATAACGATGGCGCTCAAGGTGATATCGTGTTGAAAATTGTACTTGTTTTCTCACTGCTTTCGTGTTTTTTTTGAAGTAAGGTTTGGATTTTGAATGTGATAGGAAGTATACAAGCATGGAGGAAGGAAGTTTGGGCTTATGGGAGTGCCTCCTTTAGGTTATATGCCAAGTTCGAGGTTGAAAAAAAGTGCCCAATTTTTTGAGGAGGCTTCTTCAATTGCAAGGCTGCACAACAAGCTGCTTCCTATTGCCCTTCACAAGCTTTCGAAACAGCTCAAAGAATTCAAATACGCTTTTGCAGATACCCACACGTTGCTCCTTCAAAGAATTCTAAACCCTTTACAATACGGTAACAAATTTTACTTTGCTTATTTGTCATTATTTCTTCATTGGTTTCAATGTTGTTTTGGTCTCCGTTGTACTGTGATATACCACATCGAGTGGAGAGGAGAACGAAGCATTCCTTATAAGGGTGTGAAACCTCTCCCTCGTTTTAAAACTTTGAGGGGAAACCTGGAAGGGAATGTCCAAAGAGGACTATATATGCTAGCGGTGGGCTCTGGCTGTTACAAATGATATTAGAGTCAGACACCGAGCGGTGTGCCAGCAAGTACGCTGGCCCCAAGGGGGGTGGATTGTGAGATCCAACATCGGTTGGAAAGGAGAACGAAACATTCCTTATAAGGGTATGGAAACCTATCCTTAGTGGGCGCGTTTTAAAACCTTGGAGGGAAGCCCAGAAGGAAAAGCCCAAAGAGGACAATATGTGCTAGTGGTGGGTTTGGGCTGTTACAAATGGTATTAGAGCCAGACACTGGGCAGTGTGCTAACAAGGACGTTGGGCCCTCAAGGCCGTGGATTGTGAGATCCCACATTGGTTAGAGAGGAGAACGAAACATTCCTTATAAGGGTGTCGAAACCTCTCCCTAGTGAACGCCGTTTTCAAACCATGAAGGGAAGCCTAGAAGGGAAAGCCCAAATGGTCTCCACATCCCACATCAATTAGAGAGGAAAACAAAGCATTTCTTACAAGGGTGTGGGAACCTCTCCCCAGTGGACGCGTTTTAAACTTTGAGGGGAAACTCGAAAGGGAAAACAAACTCGAAAGGGAAAACCTAAAGAAGACTATATCTGCTAGCGGTGGGCTCAGGCTGTTTCAAATGTTATTAGAGCCAGACATCGGGCAATGTGCTAGCGAGGACACTGGGACCTCAAGGGGGTGGATTGTGAGATCCCACATCAATTGGAGAGGAGAACGAAGCATTTCTTATAAGGGTGTAGAAACCTCTCCCTAGTGGACGCATTTTAAAACCTTGAAAAAGCCCAAAGAGGACAATATCCCTAGTGGACGCATTTTAAAACCTTGAAAAAGCCCAAAGAGGACAATATCTGCTACCAGTCAGTAGGCTTGAGCTATTACACCTACACTTCTAAACTGTCTATAATGGTAGCAAAACGTTTATGTTACCGAGATGCTCGGGGTCTAGAGTTAGACTATCATCGACACATTGTTAAGTGATGGTATTGTGTGAATGAAGGATTCAAGGTAGTGGATACAGCTTGCTGTGGGAGTGACGAATTTAGAGGCGTTTATAACTGTGGAAGAAAGCTGGGAAGTTTGCCCTTTACGCATTGCAAAAACCTTGAAGATCATATGTTCTTTGATTCTTTTCATCCCACTGAGAAAGTTTTCAAGCAGCTTGCAGAACAGATGTGGAGTGGAGGCCTTGAAGTTGTTAAGCCTTACAACTTCAAGCAGCTCTTTGAGTATGAACCAACCTTGGCCTCTGAGTAAGAATGTGTCCTCTTTTTTGAATCATTTAGGCTGTTTCTAGCTCGTTTTAGACTCGTTTTGTTTCTCGATTTATCTCGAACTGCGGGATTTTAGTATCGTTGGGTTTGGAACCTCGTATTGCGAGTAATGTTAGTTCATCGAGTCCGTCTTTTATTTAGAAAAAATAACTTTGAAAGGCTTTTCATTGCGTTTTTTGAACACATTACATGTAATATCATTGAGAAGCAGCTATGAAAGACTTTACATAGCGTTTTTAGGCACATATTACATGTAATATCATCGAGAAGCAACTATGAAAGACTTTTTATAGCGTTTTTAGACACGTATTACATGTAATATCATCGAGAAGTAGCTACGAAAGACTTTTCCATAGCATTTTTAGACGCATATTACATGTAATATTGTCAAGAAGCAGCTACGAAAGACTTTTCATAGTATTTTTAGACATTTATTGTATGTAATATCATTGGAAAGCAACTATGAAAGACTTTCCATAGCGTTTTTAGGCACATATTACATGTAATATCATTGAGAAGTAGCTATGAAAGACTTTTCATAGCATTTTTAGACGTGTATTACATGTATATCATCGAGAATAAGATATGAAAGACTTTCTATAGCGTTTTTAGGCACATATTACATGTAATATCATTTAGAAGTAGCTATGAAAGACTTTCTATAGTGTTTTTACGTATTACATGTAATATCATCGAGAAGTAGCTACGAAAGACTTTCCATTGCGTTTTTAGGCACATATTACATGTAATATCATCGAGAAGCAGCTATGAAAAACTTTCTATAGCGTTTTGAGACTCATATTACATGTAATATTATTGAGAAGTA

mRNA sequence

GAGAGTTGGTAGAAAAAAAAAAATAATGTTAAAAATCAAGCAAAAAGGACAGCTGCTTTTAGAAGGCAGAAATAATTTATAATAATTTCCTATAAGAACACAAGAACATCGGCCATTGCCATCAGTTTGAACTGAGAGTTGTAGAAATGATGTTTCCAAATCTGGGCAGCTGCTTCTTCTTCATTTTGTTTGCAGCTCTATGTGGTTTTCCCACAATCTCGAAGGCGGTCGGAATCCAACTCGGCGGTGCTCCGTCGTCGGAAAACCGCTTCGGGTTTTTCATATTTGGGGATTCCTATGTGGATGTCGGAAACAACAACTACATCAATACCACTAGTGACTTTCAGGCCAACTTCCCGCCTTACGGTGAAAGCTTCTTCCCGGTCGCCACCGGCAGGTTTACCGACGGCCGTAATATCCCAGATTTCTTAGGTGAGTATGCAAATTTGCCTTTGCTCCCACCATATTTGGATCCCCACAATGATCTTTATGACTATGGAGTCAATTTTGCATCTGGTGGAGGTGGTGCTTTAGCTCTAAGTCATCAAGAACAGGCCTTAGGGCTTCAAACCCAGATGAAGTTCTTCAAAAGGGTGGACAAATCACTAAGAAAGAAGCTTGGGAATGCAAGAACTCAAAGCTTCTTCTCCAACTCTGTTTTTTTGTTTAATTTCGGAGGAAACGATTACCTAAATCCGTTCGACATAAGCTACGACATATTCAAAACCATCGACGCTCAAGAACAGTACGTGAATATGGTGATCGGAAACATAACGATGGCGCTCAAGGAAGTATACAAGCATGGAGGAAGGAAGTTTGGGCTTATGGGAGTGCCTCCTTTAGGTTATATGCCAAGTTCGAGGTTGAAAAAAAGTGCCCAATTTTTTGAGGAGGCTTCTTCAATTGCAAGGCTGCACAACAAGCTGCTTCCTATTGCCCTTCACAAGCTTTCGAAACAGCTCAAAGAATTCAAATACGCTTTTGCAGATACCCACACGTTGCTCCTTCAAAGAATTCTAAACCCTTTACAATACGGATTCAAGGTAGTGGATACAGCTTGCTGTGGGAGTGACGAATTTAGAGGCGTTTATAACTGTGGAAGAAAGCTGGGAAGTTTGCCCTTTACGCATTGCAAAAACCTTGAAGATCATATGTTCTTTGATTCTTTTCATCCCACTGAGAAAGTTTTCAAGCAGCTTGCAGAACAGATGTGGAGTGGAGGCCTTGAAGTTGTTAAGCCTTACAACTTCAAGCAGCTCTTTGAGTATGAACCAACCTTGGCCTCTGAGTAAGAATGTGTCCTCTTTTTTGAATCATTTAGGCTGTTTCTAGCTCGTTTTAGACTCGTTTTGTTTCTCGATTTATCTCGAACTGCGGGATTTTAGTATCGTTGGGTTTGGAACCTCGTATTGCGAGTAATGTTAGTTCATCGAGTCCGTCTTTTATTTAGAAAAAATAACTTTGAAAGGCTTTTCATTGCGTTTTTTGAACACATTACATGTAATATCATTGAGAAGCAGCTATGAAAGACTTTACATAGCGTTTTTAGGCACATATTACATGTAATATCATCGAGAAGCAACTATGAAAGACTTTTTATAGCGTTTTTAGACACGTATTACATGTAATATCATCGAGAAGTAGCTACGAAAGACTTTTCCATAGCATTTTTAGACGCATATTACATGTAATATTGTCAAGAAGCAGCTACGAAAGACTTTTCATAGTATTTTTAGACATTTATTGTATGTAATATCATTGGAAAGCAACTATGAAAGACTTTCCATAGCGTTTTTAGGCACATATTACATGTAATATCATTGAGAAGTAGCTATGAAAGACTTTTCATAGCATTTTTAGACGTGTATTACATGTATATCATCGAGAATAAGATATGAAAGACTTTCTATAGCGTTTTTAGGCACATATTACATGTAATATCATTTAGAAGTAGCTATGAAAGACTTTCTATAGTGTTTTTACGTATTACATGTAATATCATCGAGAAGTAGCTACGAAAGACTTTCCATTGCGTTTTTAGGCACATATTACATGTAATATCATCGAGAAGCAGCTATGAAAAACTTTCTATAGCGTTTTGAGACTCATATTACATGTAATATTATTGAGAAGTA

Coding sequence (CDS)

ATGATGTTTCCAAATCTGGGCAGCTGCTTCTTCTTCATTTTGTTTGCAGCTCTATGTGGTTTTCCCACAATCTCGAAGGCGGTCGGAATCCAACTCGGCGGTGCTCCGTCGTCGGAAAACCGCTTCGGGTTTTTCATATTTGGGGATTCCTATGTGGATGTCGGAAACAACAACTACATCAATACCACTAGTGACTTTCAGGCCAACTTCCCGCCTTACGGTGAAAGCTTCTTCCCGGTCGCCACCGGCAGGTTTACCGACGGCCGTAATATCCCAGATTTCTTAGGTGAGTATGCAAATTTGCCTTTGCTCCCACCATATTTGGATCCCCACAATGATCTTTATGACTATGGAGTCAATTTTGCATCTGGTGGAGGTGGTGCTTTAGCTCTAAGTCATCAAGAACAGGCCTTAGGGCTTCAAACCCAGATGAAGTTCTTCAAAAGGGTGGACAAATCACTAAGAAAGAAGCTTGGGAATGCAAGAACTCAAAGCTTCTTCTCCAACTCTGTTTTTTTGTTTAATTTCGGAGGAAACGATTACCTAAATCCGTTCGACATAAGCTACGACATATTCAAAACCATCGACGCTCAAGAACAGTACGTGAATATGGTGATCGGAAACATAACGATGGCGCTCAAGGAAGTATACAAGCATGGAGGAAGGAAGTTTGGGCTTATGGGAGTGCCTCCTTTAGGTTATATGCCAAGTTCGAGGTTGAAAAAAAGTGCCCAATTTTTTGAGGAGGCTTCTTCAATTGCAAGGCTGCACAACAAGCTGCTTCCTATTGCCCTTCACAAGCTTTCGAAACAGCTCAAAGAATTCAAATACGCTTTTGCAGATACCCACACGTTGCTCCTTCAAAGAATTCTAAACCCTTTACAATACGGATTCAAGGTAGTGGATACAGCTTGCTGTGGGAGTGACGAATTTAGAGGCGTTTATAACTGTGGAAGAAAGCTGGGAAGTTTGCCCTTTACGCATTGCAAAAACCTTGAAGATCATATGTTCTTTGATTCTTTTCATCCCACTGAGAAAGTTTTCAAGCAGCTTGCAGAACAGATGTGGAGTGGAGGCCTTGAAGTTGTTAAGCCTTACAACTTCAAGCAGCTCTTTGAGTATGAACCAACCTTGGCCTCTGAGTAA

Protein sequence

MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFEYEPTLASE
Homology
BLAST of Cp4.1LG16g00190 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 1.4e-84
Identity = 168/367 (45.78%), Postives = 226/367 (61.58%), Query Frame = 0

Query: 13  ILFAALC-GFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYINTTSDFQANFP 72
           +L A LC   PT          G  SS+     FIFGDS  D GNNN+INT  +F+ANF 
Sbjct: 12  VLVAVLCLSLPT----------GCLSSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFW 71

Query: 73  PYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVNFASGGGGALAL 132
           PYG+S+F   TGRF+DGR IPDF+ EYA+LP++P YL+P+ND + +G NFAS G GAL  
Sbjct: 72  PYGQSYFSSPTGRFSDGRIIPDFIAEYASLPIIPAYLEPNND-FTHGANFASAGAGALIA 131

Query: 133 SHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDI 192
           SH   A+GLQTQ+++F  +    R+ LG+ +++   S++V+LF+ GGNDY +P+   Y  
Sbjct: 132 SHAGLAVGLQTQLRYFGDLVDHYRQNLGDIKSRQLLSDAVYLFSCGGNDYQSPY---YPY 191

Query: 193 FKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQFFE-EA 252
                 QEQYV++VIGN+T  +K +Y+ GGRKFG++ VP +G  P  R K+       E 
Sbjct: 192 -----TQEQYVDIVIGNMTNVIKGIYEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEV 251

Query: 253 SSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDE 312
             + RLHN+     L +L KQL+ F YA  D  T +L R+ NP +YGFK  ++ACCGS  
Sbjct: 252 DELTRLHNQAFAKRLEQLEKQLEGFVYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGP 311

Query: 313 FRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQ 372
           F G Y+CGR      F  C N  ++ FFD FHP E   +Q AE  W G   V +PYN K 
Sbjct: 312 FGGNYDCGR---IKEFGLCDNATEYFFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKA 356

Query: 373 LFEYEPT 378
           LFE +P+
Sbjct: 372 LFEGKPS 356

BLAST of Cp4.1LG16g00190 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 2.1e-80
Identity = 154/340 (45.29%), Postives = 215/340 (63.24%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYI+T S  ++N+ PYG++ F   TGR +DGR IPDF+ EYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 100 NLPLLPPYLDPH--NDLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LPL+PP L P   N  + YGVNFASGG GAL  +     + L+TQ+  FK+V++ LR K
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG+A  +   S +V+LF+ G NDY  PF  +  +F++I + E+YV+ V+GN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVY 213

Query: 220 KHGGRKFGLMGVPPLGYMPSSRL---KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
             GGRKFG++   P    P+S +    K    F+  + +  +HN+ L   L +L+ +L  
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           FKYA  D HT L +R+ +P +YGFK    ACCGS   RG+  CG ++G S  +  C+N+ 
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVT 333

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFE 374
           D++FFD FH TEK  +Q+AE +WSG   +  PYN K LFE
Sbjct: 334 DYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Cp4.1LG16g00190 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.1e-79
Identity = 153/340 (45.00%), Postives = 211/340 (62.06%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYI+T   F++N+ PYG++ F   TGR +DGR IPDF+ EYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 100 NLPLLPPYLDPHN--DLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LPL+P YL P N  + + YGV+FAS G GAL  +     + L++Q+  FK+V+K LR  
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG A+ +   S +V+LF+ G NDY  PF  +  IF++   QE YV+ V+GN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVY 215

Query: 220 KHGGRKFGLMGVPPLGYMPSSRL---KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
           K GGRKFG + +      P+S +    K    F+  + +  LHN+ L   L +L ++L  
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           FKYA  D HT L  R+ NP +YGFK    ACCG+   RG+  CG ++G S  +  C+ + 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFE 374
           D++FFD FH TEK  +Q+AE +WSG   V KPYN + LFE
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of Cp4.1LG16g00190 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 4.1e-79
Identity = 156/344 (45.35%), Postives = 215/344 (62.50%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYINT S F++N  PYG++ F   TGR +DG        E A
Sbjct: 32  NQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKA 91

Query: 100 NLPLLPPYLDPH--NDLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LP +PP L P+  N+ + YGV+FAS G GALA S     + L TQ+  FK V+KSLR +
Sbjct: 92  WLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSE 151

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG+A T+  FS +V+LF+ G NDY  PF  +   FK+ +++E++V+ VIGNIT  ++EVY
Sbjct: 152 LGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFKS-NSKEKFVDFVIGNITFVIEEVY 211

Query: 220 KHGGRKFGLMGVPPLGYMPSSRLK---KSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
           K GGRKFG + V P    P+S ++   K    F+  + +  +HNK  P  L +L +QL  
Sbjct: 212 KMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSG 271

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           F+YA  D HT L +RI +P +YGFK    ACCGS   RG+  CG ++G S  +  C+N+ 
Sbjct: 272 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 331

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFEYEPT 378
           D++F+DS H TEK  +Q+AE +W+G   V +PYN K LFE   T
Sbjct: 332 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFELRLT 367

BLAST of Cp4.1LG16g00190 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.9e-76
Identity = 152/374 (40.64%), Postives = 226/374 (60.43%), Query Frame = 0

Query: 8   SCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYINTTSDFQ 67
           S F F + ++   F     +  I   G     N    F+FGDS++D GNNNYINTT+  Q
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNG---DNNVTALFLFGDSFLDAGNNNYINTTTLDQ 75

Query: 68  ANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD-YGVNFASGGG 127
           ANFPPYG++FF + TGRF+DGR I DF+ EYANLPL+PP+L+P N     YGVNFAS G 
Sbjct: 76  ANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGA 135

Query: 128 GALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFD 187
           GAL  + Q   + L+TQ+  +K+V++  R   G   ++   S +V+L + G NDY + F 
Sbjct: 136 GALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFL 195

Query: 188 ISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL---KKS 247
            +  +     +  Q+V++VIGN+T  + E+YK GGRKFG + VP LG  P+ R+   K  
Sbjct: 196 TNQSL---PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKND 255

Query: 248 AQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKVVDT 307
                +AS +A +HN+ L   L ++ +Q+K FK++  D +  L  R+ +P ++GFK  + 
Sbjct: 256 DSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEE 315

Query: 308 ACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGG---- 367
           ACCG+ ++RGV++CG K     +  C+N +D++F+DS H T+  + Q A  +W+GG    
Sbjct: 316 ACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSD 375

Query: 368 LEVVKPYNFKQLFE 374
             VV PYN   LF+
Sbjct: 376 SLVVGPYNINNLFQ 383

BLAST of Cp4.1LG16g00190 vs. NCBI nr
Match: XP_023512752.1 (GDSL esterase/lipase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 788 bits (2034), Expect = 6.91e-288
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI
Sbjct: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN
Sbjct: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND
Sbjct: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV
Sbjct: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL
Sbjct: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360

Query: 361 EVVKPYNFKQLFEYEPTLASE 381
           EVVKPYNFKQLFEYEPTLASE
Sbjct: 361 EVVKPYNFKQLFEYEPTLASE 381

BLAST of Cp4.1LG16g00190 vs. NCBI nr
Match: XP_022928027.1 (GDSL esterase/lipase 3-like [Cucurbita moschata])

HSP 1 Score: 759 bits (1961), Expect = 9.66e-277
Identity = 369/382 (96.60%), Postives = 374/382 (97.91%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M FPNLGSCFFFILFAA CGFPTIS+AVGIQL GAPSSENRFGFFIFGDSYVDVGNNNYI
Sbjct: 1   MKFPNLGSCFFFILFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN
Sbjct: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND
Sbjct: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTIDAQEQ+VNMVIGNIT ALKEVYKHGGRKFGLMGVPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQFFEEASSIARLHNKLLPIALHKLSKQLK FKYAFADTHTLLLQRILNP +YGFKV
Sbjct: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGFKV 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQL EQMWSGGL
Sbjct: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSGGL 360

Query: 361 EVVKPYNFKQLFEYEPT-LASE 381
           EVVKPYNFKQLF+YEPT LAS+
Sbjct: 361 EVVKPYNFKQLFDYEPTTLASQ 382

BLAST of Cp4.1LG16g00190 vs. NCBI nr
Match: XP_022971689.1 (GDSL esterase/lipase 3-like [Cucurbita maxima])

HSP 1 Score: 744 bits (1920), Expect = 1.65e-270
Identity = 358/381 (93.96%), Postives = 363/381 (95.28%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M FPN  SCFFFILF A CGFPTIS+AVGIQL GAPSSENRFGFFIFGDSYVD GNNNYI
Sbjct: 1   MKFPNRSSCFFFILFVAQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN
Sbjct: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGAL LSHQEQALGLQTQM FFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND
Sbjct: 121 FASGGGGALPLSHQEQALGLQTQMNFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTID QEQ+VNMVIGNIT  LKEVYKHGGRKFGLMGVPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIDTQEQFVNMVIGNITTVLKEVYKHGGRKFGLMGVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQ+FEEASSIARLHNKLLPIALHKLSKQLK FKYAFAD H LLLQRILNP QYGFKV
Sbjct: 241 KKSAQYFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADVHMLLLQRILNPSQYGFKV 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRKLGS PFTHCKN+EDHMFFDS+HPTEKVFKQLAEQMWSGGL
Sbjct: 301 VDTACCGSDEFRGVYNCGRKLGSFPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQMWSGGL 360

Query: 361 EVVKPYNFKQLFEYEPTLASE 381
           EVV PYNFKQLFEYEPTLASE
Sbjct: 361 EVVTPYNFKQLFEYEPTLASE 381

BLAST of Cp4.1LG16g00190 vs. NCBI nr
Match: XP_038900701.1 (GDSL lipase-like [Benincasa hispida])

HSP 1 Score: 654 bits (1686), Expect = 5.78e-235
Identity = 313/380 (82.37%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M FP LG CFF ILF+A      +S+A  IQL G PSSE+RFG FIFGDSYVD GNNNYI
Sbjct: 1   MKFPKLGICFF-ILFSA------VSEAARIQLPGIPSSESRFGLFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFF + TGRFTDGRNIPDFLGEYANLPLLPPYLDPHN+LY++GVN
Sbjct: 61  NTTSDFQANFPPYGESFFQIPTGRFTDGRNIPDFLGEYANLPLLPPYLDPHNNLYEHGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGALALSHQ+QA+GLQTQ+KFFK V+KSLRKKLG AR+++F SNSVFLFNFGGND
Sbjct: 121 FASGGGGALALSHQDQAIGLQTQLKFFKNVEKSLRKKLGYARSKTFLSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTI+AQE YVNMVIGNIT+A+KEVYK+GGRKFG + VPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIEAQEHYVNMVIGNITIAVKEVYKYGGRKFGFLAVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQFFEEASSIAR+HNKLLPIAL KLSKQLK FKY FAD HTLLLQRILNP +YGFK+
Sbjct: 241 KKSAQFFEEASSIARIHNKLLPIALQKLSKQLKGFKYTFADVHTLLLQRILNPTKYGFKI 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRK G LP+THC+NLEDHMFFDSFHPT+KVFKQLAE+ WSG  
Sbjct: 301 VDTACCGSDEFRGVYNCGRKFGDLPYTHCENLEDHMFFDSFHPTQKVFKQLAEEFWSGDA 360

Query: 361 EVVKPYNFKQLFEYEPTLAS 380
           E+VKP NFKQLF+Y+  LAS
Sbjct: 361 EIVKPLNFKQLFQYDSNLAS 373

BLAST of Cp4.1LG16g00190 vs. NCBI nr
Match: XP_016902155.1 (PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo] >KAA0045892.1 GDSL esterase/lipase 1-like [Cucumis melo var. makuwa] >TYK13695.1 GDSL esterase/lipase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 622 bits (1604), Expect = 2.15e-222
Identity = 305/384 (79.43%), Postives = 336/384 (87.50%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M F N    FF I FAA      +S+A  IQL G   S+ RFGFFIFGDSYVD GNNNYI
Sbjct: 1   MKFSNFCISFFIIFFAA------VSEAASIQLPGNSLSDTRFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFP--VATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120
            TTSDFQANFPPYGESFFP  +ATGRFTDGRNIPDFLGEYANLPL+PPYLDPHNDLYDYG
Sbjct: 61  ITTSDFQANFPPYGESFFPNRIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYG 120

Query: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180
           VNFASGGGGALALSHQEQA+GLQTQM FF++V KSLR KLG+AR++SF SNSVFLFNFGG
Sbjct: 121 VNFASGGGGALALSHQEQAIGLQTQMVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSS 240
           NDYLNPFDISYDIFKTI+AQEQYVNMV+GNIT+A+KEVYK+GGRKFG++ VPPLGYMPSS
Sbjct: 181 NDYLNPFDISYDIFKTIEAQEQYVNMVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSS 240

Query: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGF 300
           RLKKSAQFFEE+SSIAR+HNK + IAL KLSKQLK FKY FAD HT LLQRI NP +YGF
Sbjct: 241 RLKKSAQFFEESSSIARIHNKFILIALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGF 300

Query: 301 KVVDTACCGSDEFRGVYNCGRK-LGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWS 360
           KVVDTACCGSDEFRGVYNCGRK  GSLP+THC+NLEDHMFFDSFHPT+KVFKQLA++ WS
Sbjct: 301 KVVDTACCGSDEFRGVYNCGRKDFGSLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWS 360

Query: 361 GGLEVVKPYNFKQLFEYEP-TLAS 380
           G +++VKP NFKQLF  +  TLAS
Sbjct: 361 GDVDIVKPLNFKQLFSCDDSTLAS 378

BLAST of Cp4.1LG16g00190 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 759 bits (1961), Expect = 4.67e-277
Identity = 369/382 (96.60%), Postives = 374/382 (97.91%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M FPNLGSCFFFILFAA CGFPTIS+AVGIQL GAPSSENRFGFFIFGDSYVDVGNNNYI
Sbjct: 1   MKFPNLGSCFFFILFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN
Sbjct: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND
Sbjct: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTIDAQEQ+VNMVIGNIT ALKEVYKHGGRKFGLMGVPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQFFEEASSIARLHNKLLPIALHKLSKQLK FKYAFADTHTLLLQRILNP +YGFKV
Sbjct: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRYGFKV 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQL EQMWSGGL
Sbjct: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMWSGGL 360

Query: 361 EVVKPYNFKQLFEYEPT-LASE 381
           EVVKPYNFKQLF+YEPT LAS+
Sbjct: 361 EVVKPYNFKQLFDYEPTTLASQ 382

BLAST of Cp4.1LG16g00190 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 744 bits (1920), Expect = 8.00e-271
Identity = 358/381 (93.96%), Postives = 363/381 (95.28%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M FPN  SCFFFILF A CGFPTIS+AVGIQL GAPSSENRFGFFIFGDSYVD GNNNYI
Sbjct: 1   MKFPNRSSCFFFILFVAQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120
           NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN
Sbjct: 61  NTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYGVN 120

Query: 121 FASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180
           FASGGGGAL LSHQEQALGLQTQM FFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND
Sbjct: 121 FASGGGGALPLSHQEQALGLQTQMNFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGND 180

Query: 181 YLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL 240
           YLNPFDISYDIFKTID QEQ+VNMVIGNIT  LKEVYKHGGRKFGLMGVPPLGYMPSSRL
Sbjct: 181 YLNPFDISYDIFKTIDTQEQFVNMVIGNITTVLKEVYKHGGRKFGLMGVPPLGYMPSSRL 240

Query: 241 KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKV 300
           KKSAQ+FEEASSIARLHNKLLPIALHKLSKQLK FKYAFAD H LLLQRILNP QYGFKV
Sbjct: 241 KKSAQYFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADVHMLLLQRILNPSQYGFKV 300

Query: 301 VDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGGL 360
           VDTACCGSDEFRGVYNCGRKLGS PFTHCKN+EDHMFFDS+HPTEKVFKQLAEQMWSGGL
Sbjct: 301 VDTACCGSDEFRGVYNCGRKLGSFPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQMWSGGL 360

Query: 361 EVVKPYNFKQLFEYEPTLASE 381
           EVV PYNFKQLFEYEPTLASE
Sbjct: 361 EVVTPYNFKQLFEYEPTLASE 381

BLAST of Cp4.1LG16g00190 vs. ExPASy TrEMBL
Match: A0A5D3CQ75 (GDSL esterase/lipase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002190 PE=3 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 1.04e-222
Identity = 305/384 (79.43%), Postives = 336/384 (87.50%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M F N    FF I FAA      +S+A  IQL G   S+ RFGFFIFGDSYVD GNNNYI
Sbjct: 1   MKFSNFCISFFIIFFAA------VSEAASIQLPGNSLSDTRFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFP--VATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120
            TTSDFQANFPPYGESFFP  +ATGRFTDGRNIPDFLGEYANLPL+PPYLDPHNDLYDYG
Sbjct: 61  ITTSDFQANFPPYGESFFPNRIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYG 120

Query: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180
           VNFASGGGGALALSHQEQA+GLQTQM FF++V KSLR KLG+AR++SF SNSVFLFNFGG
Sbjct: 121 VNFASGGGGALALSHQEQAIGLQTQMVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSS 240
           NDYLNPFDISYDIFKTI+AQEQYVNMV+GNIT+A+KEVYK+GGRKFG++ VPPLGYMPSS
Sbjct: 181 NDYLNPFDISYDIFKTIEAQEQYVNMVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSS 240

Query: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGF 300
           RLKKSAQFFEE+SSIAR+HNK + IAL KLSKQLK FKY FAD HT LLQRI NP +YGF
Sbjct: 241 RLKKSAQFFEESSSIARIHNKFILIALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGF 300

Query: 301 KVVDTACCGSDEFRGVYNCGRK-LGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWS 360
           KVVDTACCGSDEFRGVYNCGRK  GSLP+THC+NLEDHMFFDSFHPT+KVFKQLA++ WS
Sbjct: 301 KVVDTACCGSDEFRGVYNCGRKDFGSLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWS 360

Query: 361 GGLEVVKPYNFKQLFEYEP-TLAS 380
           G +++VKP NFKQLF  +  TLAS
Sbjct: 361 GDVDIVKPLNFKQLFSCDDSTLAS 378

BLAST of Cp4.1LG16g00190 vs. ExPASy TrEMBL
Match: A0A1S4E2F5 (GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103497528 PE=3 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 1.04e-222
Identity = 305/384 (79.43%), Postives = 336/384 (87.50%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M F N    FF I FAA      +S+A  IQL G   S+ RFGFFIFGDSYVD GNNNYI
Sbjct: 1   MKFSNFCISFFIIFFAA------VSEAASIQLPGNSLSDTRFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFP--VATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120
            TTSDFQANFPPYGESFFP  +ATGRFTDGRNIPDFLGEYANLPL+PPYLDPHNDLYDYG
Sbjct: 61  ITTSDFQANFPPYGESFFPNRIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYG 120

Query: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180
           VNFASGGGGALALSHQEQA+GLQTQM FF++V KSLR KLG+AR++SF SNSVFLFNFGG
Sbjct: 121 VNFASGGGGALALSHQEQAIGLQTQMVFFRKVVKSLRNKLGHARSRSFLSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSS 240
           NDYLNPFDISYDIFKTI+AQEQYVNMV+GNIT+A+KEVYK+GGRKFG++ VPPLGYMPSS
Sbjct: 181 NDYLNPFDISYDIFKTIEAQEQYVNMVVGNITIAIKEVYKYGGRKFGVLAVPPLGYMPSS 240

Query: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGF 300
           RLKKSAQFFEE+SSIAR+HNK + IAL KLSKQLK FKY FAD HT LLQRI NP +YGF
Sbjct: 241 RLKKSAQFFEESSSIARIHNKFILIALEKLSKQLKGFKYTFADVHTALLQRIQNPTKYGF 300

Query: 301 KVVDTACCGSDEFRGVYNCGRK-LGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWS 360
           KVVDTACCGSDEFRGVYNCGRK  GSLP+THC+NLEDHMFFDSFHPT+KVFKQLA++ WS
Sbjct: 301 KVVDTACCGSDEFRGVYNCGRKDFGSLPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWS 360

Query: 361 GGLEVVKPYNFKQLFEYEP-TLAS 380
           G +++VKP NFKQLF  +  TLAS
Sbjct: 361 GDVDIVKPLNFKQLFSCDDSTLAS 378

BLAST of Cp4.1LG16g00190 vs. ExPASy TrEMBL
Match: A0A0A0LQ38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296110 PE=3 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 2.02e-222
Identity = 299/383 (78.07%), Postives = 335/383 (87.47%), Query Frame = 0

Query: 1   MMFPNLGSCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYI 60
           M F N   C FFI FAA      + +A  IQL G  S +  FGFFIFGDSYVD GNNNYI
Sbjct: 1   MEFSNFCICCFFIFFAA------VLEAARIQLPGNSSPDTSFGFFIFGDSYVDAGNNNYI 60

Query: 61  NTTSDFQANFPPYGESFFP--VATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYDYG 120
            TTSDFQANFPPYGESFFP  +ATGRFTDGRNIPDFLGEYANLPL+PPYLDPHNDLYDYG
Sbjct: 61  ITTSDFQANFPPYGESFFPNPIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHNDLYDYG 120

Query: 121 VNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGG 180
            NFASGGGGA+A+SHQEQA+GLQTQM+FF++V+KSLR KLG+AR++SF SNSVFLFNFGG
Sbjct: 121 ANFASGGGGAIAMSHQEQAIGLQTQMEFFRKVEKSLRNKLGHARSKSFLSNSVFLFNFGG 180

Query: 181 NDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSS 240
           NDYLNPFDISYDIFKTI+AQEQ+VNMV+GNIT+A+KEVY++GGRKFG++ VPPLGYMPSS
Sbjct: 181 NDYLNPFDISYDIFKTIEAQEQFVNMVVGNITIAIKEVYEYGGRKFGVLAVPPLGYMPSS 240

Query: 241 RLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGF 300
           RLKKSAQFFEEASSIAR+HNK L IAL KLSKQLK FKY FAD HT LLQRI NP +YGF
Sbjct: 241 RLKKSAQFFEEASSIARIHNKFLLIALEKLSKQLKGFKYTFADVHTALLQRIQNPTEYGF 300

Query: 301 KVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSG 360
           KVVDTACCGSDEFRG+YNCGR+ GS P+THC+NLEDHMFFDSFHPT+KVFKQLA++ WSG
Sbjct: 301 KVVDTACCGSDEFRGIYNCGREFGSSPYTHCQNLEDHMFFDSFHPTQKVFKQLADEFWSG 360

Query: 361 GLEVVKPYNFKQLFEYEP-TLAS 380
             ++VKP NFKQLF Y+  TLAS
Sbjct: 361 DEDIVKPVNFKQLFHYDDSTLAS 377

BLAST of Cp4.1LG16g00190 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 300.8 bits (769), Expect = 1.5e-81
Identity = 154/340 (45.29%), Postives = 215/340 (63.24%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYI+T S  ++N+ PYG++ F   TGR +DGR IPDF+ EYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 100 NLPLLPPYLDPH--NDLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LPL+PP L P   N  + YGVNFASGG GAL  +     + L+TQ+  FK+V++ LR K
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG+A  +   S +V+LF+ G NDY  PF  +  +F++I + E+YV+ V+GN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVY 213

Query: 220 KHGGRKFGLMGVPPLGYMPSSRL---KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
             GGRKFG++   P    P+S +    K    F+  + +  +HN+ L   L +L+ +L  
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           FKYA  D HT L +R+ +P +YGFK    ACCGS   RG+  CG ++G S  +  C+N+ 
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVT 333

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFE 374
           D++FFD FH TEK  +Q+AE +WSG   +  PYN K LFE
Sbjct: 334 DYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of Cp4.1LG16g00190 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 296.6 bits (758), Expect = 2.9e-80
Identity = 156/344 (45.35%), Postives = 215/344 (62.50%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYINT S F++N  PYG++ F   TGR +DG        E A
Sbjct: 32  NQAALFVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKA 91

Query: 100 NLPLLPPYLDPH--NDLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LP +PP L P+  N+ + YGV+FAS G GALA S     + L TQ+  FK V+KSLR +
Sbjct: 92  WLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSE 151

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG+A T+  FS +V+LF+ G NDY  PF  +   FK+ +++E++V+ VIGNIT  ++EVY
Sbjct: 152 LGDAETKRVFSRAVYLFHIGANDYFYPFSANSSTFKS-NSKEKFVDFVIGNITFVIEEVY 211

Query: 220 KHGGRKFGLMGVPPLGYMPSSRLK---KSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
           K GGRKFG + V P    P+S ++   K    F+  + +  +HNK  P  L +L +QL  
Sbjct: 212 KMGGRKFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSG 271

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           F+YA  D HT L +RI +P +YGFK    ACCGS   RG+  CG ++G S  +  C+N+ 
Sbjct: 272 FRYALHDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVT 331

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLFEYEPT 378
           D++F+DS H TEK  +Q+AE +W+G   V +PYN K LFE   T
Sbjct: 332 DYLFYDSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALFELRLT 367

BLAST of Cp4.1LG16g00190 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 152/374 (40.64%), Postives = 226/374 (60.43%), Query Frame = 0

Query: 8   SCFFFILFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGNNNYINTTSDFQ 67
           S F F + ++   F     +  I   G     N    F+FGDS++D GNNNYINTT+  Q
Sbjct: 16  SSFIFFIVSSTILFLAGKSSAKISHNG---DNNVTALFLFGDSFLDAGNNNYINTTTLDQ 75

Query: 68  ANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD-YGVNFASGGG 127
           ANFPPYG++FF + TGRF+DGR I DF+ EYANLPL+PP+L+P N     YGVNFAS G 
Sbjct: 76  ANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASAGA 135

Query: 128 GALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFD 187
           GAL  + Q   + L+TQ+  +K+V++  R   G   ++   S +V+L + G NDY + F 
Sbjct: 136 GALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSSIFL 195

Query: 188 ISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMPSSRL---KKS 247
            +  +     +  Q+V++VIGN+T  + E+YK GGRKFG + VP LG  P+ R+   K  
Sbjct: 196 TNQSL---PISMSQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQPKND 255

Query: 248 AQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQYGFKVVDT 307
                +AS +A +HN+ L   L ++ +Q+K FK++  D +  L  R+ +P ++GFK  + 
Sbjct: 256 DSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKEGEE 315

Query: 308 ACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMWSGG---- 367
           ACCG+ ++RGV++CG K     +  C+N +D++F+DS H T+  + Q A  +W+GG    
Sbjct: 316 ACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGHMSD 375

Query: 368 LEVVKPYNFKQLFE 374
             VV PYN   LF+
Sbjct: 376 SLVVGPYNINNLFQ 383

BLAST of Cp4.1LG16g00190 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 282.7 bits (722), Expect = 4.3e-76
Identity = 145/325 (44.62%), Postives = 202/325 (62.15%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F+FGDS  D GNNNYI+T   F++N+ PYG++ F   TGR +DGR IPDF+ EYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 100 NLPLLPPYLDPHN--DLYDYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKK 159
            LPL+P YL P N  + + YGV+FAS G GAL  +     + L++Q+  FK+V+K LR  
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 160 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVY 219
           LG A+ +   S +V+LF+ G NDY  PF  +  IF++   QE YV+ V+GN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVY 215

Query: 220 KHGGRKFGLMGVPPLGYMPSSRL---KKSAQFFEEASSIARLHNKLLPIALHKLSKQLKE 279
           K GGRKFG + +      P+S +    K    F+  + +  LHN+ L   L +L ++L  
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 280 FKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHCKNLE 339
           FKYA  D HT L  R+ NP +YGFK    ACCG+   RG+  CG ++G S  +  C+ + 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 340 DHMFFDSFHPTEKVFKQLAEQMWSG 359
           D++FFD FH TEK  +Q+AE +WSG
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSG 359

BLAST of Cp4.1LG16g00190 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 274.2 bits (700), Expect = 1.5e-73
Identity = 146/343 (42.57%), Postives = 201/343 (58.60%), Query Frame = 0

Query: 40  NRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYA 99
           N+   F FGDS  + GNNNY ++ S F++NF PYG++ F   TGR +DGR + DF+ EYA
Sbjct: 34  NQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYA 93

Query: 100 NLPLLPPYLDP--HNDLYDYGVNFASGGGGALALSHQEQALGLQ----TQMKFFKRVDKS 159
            LPL+PP L P   N    YG+NFA+   G  A +       L     TQ+  FK V+K+
Sbjct: 94  WLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKT 153

Query: 160 LRKKLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMAL 219
           LR  LG+A  +   S +V+LF+ G NDY  PF  +   F     +E++++ VIGN T  +
Sbjct: 154 LRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN-TTKERFIDFVIGNTTTVI 213

Query: 220 KEVYKHGGRKFGLMGVPPLGYMPSSRLKKSAQF---FEEASSIARLHNKLLPIALHKLSK 279
           +E+YK G RKFG + + P G  PS+ +  S +    FE  + +  LHN+  P  L +L +
Sbjct: 214 EELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTELINLHNQEFPKVLRRLER 273

Query: 280 QLKEFKYAFADTHTLLLQRILNPLQYGFKVVDTACCGSDEFRGVYNCGRKLG-SLPFTHC 339
           +L  FKYA  D HT L QRI NP +YGFK  + ACCGS   RG+  CG + G S  +  C
Sbjct: 274 RLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLC 333

Query: 340 KNLEDHMFFDSFHPTEKVFKQLAEQMWSGGLEVVKPYNFKQLF 373
           +N +D++FFD  H TE   +Q+AE +WSG   V  PYN K LF
Sbjct: 334 ENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
H6U1I81.4e-8445.78GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1[more]
Q9FLN02.1e-8045.29GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1[more]
Q9SYF01.1e-7945.00GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1[more]
Q9SYF54.1e-7945.35GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2[more]
Q9SSA71.9e-7640.64GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_023512752.16.91e-288100.00GDSL esterase/lipase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022928027.19.66e-27796.60GDSL esterase/lipase 3-like [Cucurbita moschata][more]
XP_022971689.11.65e-27093.96GDSL esterase/lipase 3-like [Cucurbita maxima][more]
XP_038900701.15.78e-23582.37GDSL lipase-like [Benincasa hispida][more]
XP_016902155.12.15e-22279.43PREDICTED: GDSL esterase/lipase 1-like [Cucumis melo] >KAA0045892.1 GDSL esteras... [more]
Match NameE-valueIdentityDescription
A0A6J1EIS04.67e-27796.60GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 S... [more]
A0A6J1I9A08.00e-27193.96GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=... [more]
A0A5D3CQ751.04e-22279.43GDSL esterase/lipase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S4E2F51.04e-22279.43GDSL esterase/lipase 1-like OS=Cucumis melo OX=3656 GN=LOC103497528 PE=3 SV=1[more]
A0A0A0LQ382.02e-22278.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296110 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G40990.11.5e-8145.29GDSL lipase 1 [more]
AT1G53990.12.9e-8045.35GDSL-motif lipase 3 [more]
AT1G53920.11.3e-7740.64GDSL-motif lipase 5 [more]
AT1G53940.14.3e-7644.62GDSL-motif lipase 2 [more]
AT3G14225.11.5e-7342.57GDSL-motif lipase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 45..354
e-value: 5.0E-25
score: 89.3
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 40..358
e-value: 8.6E-68
score: 231.1
IPR044552GDSL esterase/lipase GLIP1-5/GLL25PANTHERPTHR45966GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 10..373
NoneNo IPR availablePANTHERPTHR45966:SF4GDSL ESTERASE/LIPASE 5coord: 10..373
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 44..357
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 44..358
e-value: 1.83824E-100
score: 298.375

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG16g00190.1Cp4.1LG16g00190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016298 lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds