Cp4.1LG15g08460 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g08460
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPLASMODESMATA CALLOSE-BINDING PROTEIN 5-like
LocationCp4.1LG15: 8311923 .. 8313098 (-)
RNA-Seq ExpressionCp4.1LG15g08460
SyntenyCp4.1LG15g08460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGGGCTTAATCCTGAAAATCATCCAAAACTGATTAGTAGTGGAATCCGTTTCTGCCATCTCCGTAACGGTCACTTCCCCTGTTCCCTCTTAAACTCCCCTTTGTTTTCTCTCTCTAAAGCCCCCACTCTCCAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAGCCCCCAATCTCCAAATCTCTCCCTCCCCGTCGAAAATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGTGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCTGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGTGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGTAAGCACGGCATGACCGACGACAGTTGCTTCTTCCAAAACGCCGCGGCTATCACCTCCTTAAACCCTAGTCTGTTTCTTCACTCCGATCTCTCAGTTTCTTAACTGAATCTGTTCTTTAATTCAAAACCTTTCCAGATCTGTAACTAAAATGCTATTTGAATTCGTGTTCTGATTTTGATCTCTCTTGTTTTCAGGCTACGGAAATTGCAGAATCCCAGCTAGGTAAATTTCATTGTTGATCTTTAAAATAATGTGGATCATGTTTGAAATCATCATCCACCAATGATTCTGCAATTATTTTTCTTCTTTTTTTTAGTGTTAATTGGATTTGATTTCTCATTTTATGGCGCAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCATGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTGTTTGAATTTGTATTTTTTATTAAAGCAAATATTGGTTATTAGATGTCTCAAATTCTTATTTTATATGATTTTTTTTAATTACTTGGGGCAAAAGAAAAAGATTTTCTGTATAAAGGGTTTAGCAAAAGTTAAAACATAATTTTATAAATAATTTCATAATAA

mRNA sequence

AGGGCTTAATCCTGAAAATCATCCAAAACTGATTAGTAGTGGAATCCGTTTCTGCCATCTCCGTAACGGTCACTTCCCCTGTTCCCTCTTAAACTCCCCTTTGTTTTCTCTCTCTAAAGCCCCCACTCTCCAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAAAGCCCCCAATCTCCAAATCTCTCCCTCCCCGTCGAAAATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGTGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCTGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGTGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGTAAGCACGGCATGACCGACGACAGTTGCTTCTTCCAAAACGCCGCGGCTATCACCTCCTTAAACCCTAGCTACGGAAATTGCAGAATCCCAGCTAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCATGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTGTTTGAATTTGTATTTTTTATTAAAGCAAATATTGGTTATTAGATGTCTCAAATTCTTATTTTATATGATTTTTTTTAATTACTTGGGGCAAAAGAAAAAGATTTTCTGTATAAAGGGTTTAGCAAAAGTTAAAACATAATTTTATAAATAATTTCATAATAA

Coding sequence (CDS)

ATGGCCAATAATTTTGCGCTCTTTCTTCTCATTCTTCTGATTCTCGGCAGCAGCGCGGCGGAGTCCCGGCAGCCGTCCACCAGGGACCAAAGCGGCGCGACGGAGCTCTGGTGTGTGGCCAAGAACAACGCCGACGACGCTTCTCTGCAATCCGCCCTCGACTGGGCCTGCGGCGCTGGCGGTGCCGACTGTTCACCAATCCAGCCCGGTGGTGCATGCTACGACGCATCCAATGTCCAGAATATGGCCTCCTTCGCCTTCAACGACTACTTCCGTAAGCACGGCATGACCGACGACAGTTGCTTCTTCCAAAACGCCGCGGCTATCACCTCCTTAAACCCTAGCTACGGAAATTGCAGAATCCCAGCTAGTTCGTTGAGAAATGGGAACTTTTCAAGCCAGACACCATCGGTAGGGTTGGGGCCGAGTGAAGACATAAGCGGGTCGGGTCGGAAATCGGGCCGGTCATGGGTTTGGCCTATGGTAATCGGGTATTTGTTTTTTAGAATTGTGTTTCAATCTCAAATAGGAAGCTCTAGTTTTTGTGTTTGA

Protein sequence

MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSSFCV
Homology
BLAST of Cp4.1LG15g08460 vs. ExPASy Swiss-Prot
Match: Q9M2K6 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5 PE=2 SV=2)

HSP 1 Score: 142.1 bits (357), Expect = 6.1e-33
Identity = 76/152 (50.00%), Postives = 99/152 (65.13%), Query Frame = 0

Query: 10  LILLILGSSAAESRQPSTRDQSG---ATELWCVAKNNADDASLQSALDWACGAGGADCSP 69
           L L IL ++AA     +T    G     ELWCVAKNNA+D+SLQ+A++WACG GGADC P
Sbjct: 11  LRLSILAATAAMLMVITTAQIGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGP 70

Query: 70  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 129
           IQ GG C D ++VQ MASF FN+Y+ K+G  D++C F N AA+TSLNPS G C+ P+S  
Sbjct: 71  IQQGGPCNDPTDVQKMASFVFNNYYLKNGEEDEACNFNNNAALTSLNPSQGTCKYPSSKG 130

Query: 130 RNGNFSSQTPSVGLGPSEDISGSGRKSGRSWV 159
            N    +   S+G G + D+S  GR    SW+
Sbjct: 131 ANNGRLADDTSMGAGQA-DMSRGGRPISSSWM 161

BLAST of Cp4.1LG15g08460 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 99.8 bits (247), Expect = 3.5e-20
Identity = 44/90 (48.89%), Postives = 62/90 (68.89%), Query Frame = 0

Query: 35  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRKH 94
           + +C+A +  D  +LQ+ALDWACG G ++CS IQPG +CY  +NV+  ASFAFN Y++K 
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 95  GMTDDSCFFQNAAAITSLNPSYGNCRIPAS 125
           G    SC F+  A IT+ +PS+G+C  P S
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFPGS 468

BLAST of Cp4.1LG15g08460 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 95.9 bits (237), Expect = 5.0e-19
Identity = 59/162 (36.42%), Postives = 82/162 (50.62%), Query Frame = 0

Query: 15  LGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACY 74
           L  S++ S+ PS +  S     WCV K  A +  LQ++LDWACG  G DC  IQPGGAC+
Sbjct: 345 LAKSSSSSQTPSGKVTSSG---WCVPKKGATNEELQASLDWACG-HGIDCGAIQPGGACF 404

Query: 75  DASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSLRNGNFSSQ 134
           + +NV + A++A N YF+K       C F   A +TS NPSY NC  P      G     
Sbjct: 405 EPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGGGGGGGGGGGG 464

Query: 135 TPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQI 177
           + +V    ++ +S    +       P V   L F ++F S I
Sbjct: 465 SKAV---MNKYVSSDKVEKKNGATEPKVSSSLSFLLIFLSLI 499

BLAST of Cp4.1LG15g08460 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.3e-18
Identity = 50/119 (42.02%), Postives = 70/119 (58.82%), Query Frame = 0

Query: 34  TELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRK 93
           + ++CVAK +ADD  L   L+WACG G A+C+ IQPG  CY  ++V++ ASFAFNDY++K
Sbjct: 359 SSMFCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQK 418

Query: 94  HGMTDDSCFFQNAAAITSLNPSYGNCRIPAS---SLRNGNFSSQTPSVGLGPSEDISGS 150
                 +C F   A  T+ +PSY  C    S   +  NGNF    P   LGP+  + G+
Sbjct: 419 MKSAGGTCDFDGTAITTTRDPSYRTCAYTGSLNANATNGNF----PPDALGPASPLGGN 473

BLAST of Cp4.1LG15g08460 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.1e-17
Identity = 39/88 (44.32%), Postives = 54/88 (61.36%), Query Frame = 0

Query: 32  GATELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYF 91
           G T+ WC+A + A    LQ+ALDWACG G  DCS +QP   C++   V + AS+AFN Y+
Sbjct: 386 GGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYY 445

Query: 92  RKHGMTDDSCFFQNAAAITSLNPSYGNC 120
           ++ G +   C F  A+     +PSYGNC
Sbjct: 446 QQSGASSIDCSFNGASVEVDKDPSYGNC 473

BLAST of Cp4.1LG15g08460 vs. NCBI nr
Match: XP_023554742.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 372 bits (955), Expect = 4.78e-130
Identity = 183/183 (100.00%), Postives = 183/183 (100.00%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

Query: 181 FCV 183
           FCV
Sbjct: 181 FCV 183

BLAST of Cp4.1LG15g08460 vs. NCBI nr
Match: XP_022952645.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata])

HSP 1 Score: 372 bits (954), Expect = 6.78e-130
Identity = 182/183 (99.45%), Postives = 183/183 (100.00%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

Query: 181 FCV 183
           FC+
Sbjct: 181 FCI 183

BLAST of Cp4.1LG15g08460 vs. NCBI nr
Match: XP_022969385.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita maxima])

HSP 1 Score: 358 bits (920), Expect = 1.04e-124
Identity = 176/183 (96.17%), Postives = 180/183 (98.36%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTR+QSGAT+LWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRNQSGATDLWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYG+CR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGSCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180
           IPASSLRNGNF SQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGS S
Sbjct: 121 IPASSLRNGNFPSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSPS 180

Query: 181 FCV 183
           F +
Sbjct: 181 FFI 183

BLAST of Cp4.1LG15g08460 vs. NCBI nr
Match: XP_023554741.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330 bits (846), Expect = 2.46e-113
Identity = 161/162 (99.38%), Postives = 162/162 (100.00%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMV 162
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPM+
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMI 162

BLAST of Cp4.1LG15g08460 vs. NCBI nr
Match: KAG6572411.1 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 313 bits (802), Expect = 1.54e-106
Identity = 155/156 (99.36%), Postives = 156/156 (100.00%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRS 156
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGR+
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRA 156

BLAST of Cp4.1LG15g08460 vs. ExPASy TrEMBL
Match: A0A6J1GMC7 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LOC111455276 PE=4 SV=1)

HSP 1 Score: 372 bits (954), Expect = 3.28e-130
Identity = 182/183 (99.45%), Postives = 183/183 (100.00%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180
           IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS
Sbjct: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180

Query: 181 FCV 183
           FC+
Sbjct: 181 FCI 183

BLAST of Cp4.1LG15g08460 vs. ExPASy TrEMBL
Match: A0A6J1HZS5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC111468407 PE=4 SV=1)

HSP 1 Score: 358 bits (920), Expect = 5.03e-125
Identity = 176/183 (96.17%), Postives = 180/183 (98.36%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           MANNFALFLLILLILGSSAAESRQPSTR+QSGAT+LWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MANNFALFLLILLILGSSAAESRQPSTRNQSGATDLWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYG+CR
Sbjct: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGSCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSSS 180
           IPASSLRNGNF SQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGS S
Sbjct: 121 IPASSLRNGNFPSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVFQSQIGSPS 180

Query: 181 FCV 183
           F +
Sbjct: 181 FFI 183

BLAST of Cp4.1LG15g08460 vs. ExPASy TrEMBL
Match: A0A6J1JNE0 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486269 PE=4 SV=1)

HSP 1 Score: 293 bits (749), Expect = 4.14e-99
Identity = 143/172 (83.14%), Postives = 155/172 (90.12%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           M  NFALFLLI+ IL  SAAE + PS + Q GATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MPINFALFLLIVPIL-CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           GADCSPIQP GACYDASNVQNMASFAFNDYF KHGMTDDSCFFQN+AA+TSLNPSYGNCR
Sbjct: 61  GADCSPIQPSGACYDASNVQNMASFAFNDYFLKHGMTDDSCFFQNSAAVTSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVF 172
            P+SSLRNGNFSSQ+ SVGLGPSED+SGSGR+S R WVWP+ IGYLFF+I+F
Sbjct: 121 FPSSSLRNGNFSSQSTSVGLGPSEDLSGSGRRSSRIWVWPIAIGYLFFKIMF 171

BLAST of Cp4.1LG15g08460 vs. ExPASy TrEMBL
Match: A0A6J1EY09 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437228 PE=4 SV=1)

HSP 1 Score: 288 bits (737), Expect = 2.79e-97
Identity = 141/172 (81.98%), Postives = 154/172 (89.53%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAG 60
           M  + ALFLLI LI+  SAAE + PS + Q GATELWCVAKNNADDASLQSALDWACGAG
Sbjct: 1   MEIDLALFLLIALII-CSAAEPQHPSIKGQGGATELWCVAKNNADDASLQSALDWACGAG 60

Query: 61  GADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCR 120
           G+DCSPIQP GACYDAS+VQ MASFAFNDYF KHGMTDDSCFFQN+AAITSLNPSYGNCR
Sbjct: 61  GSDCSPIQPSGACYDASSVQTMASFAFNDYFLKHGMTDDSCFFQNSAAITSLNPSYGNCR 120

Query: 121 IPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIVF 172
            P+SSLRNGNFSSQ+ SVGLGPSED+SGSGRKSGR WVWP+ IGYLFF+I+F
Sbjct: 121 FPSSSLRNGNFSSQSTSVGLGPSEDLSGSGRKSGRIWVWPIAIGYLFFKIMF 171

BLAST of Cp4.1LG15g08460 vs. ExPASy TrEMBL
Match: A0A1S3BN12 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 PE=4 SV=1)

HSP 1 Score: 285 bits (730), Expect = 3.15e-96
Identity = 141/172 (81.98%), Postives = 154/172 (89.53%), Query Frame = 0

Query: 1   MANNFALFLLILLILGSSAAESRQPSTRDQSGA-TELWCVAKNNADDASLQSALDWACGA 60
           M  NF+LFLLILLI  ++A   R+PS RDQSGA TELWCVAKNNADDASLQSALDWACGA
Sbjct: 1   MPINFSLFLLILLIPCTAADPWRRPSIRDQSGAATELWCVAKNNADDASLQSALDWACGA 60

Query: 61  GGADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNC 120
           GGADCSPIQPGG+CYDA++VQNMASFAFNDYFRKHGMTDDSCFFQN+AAITSLNPS+GNC
Sbjct: 61  GGADCSPIQPGGSCYDATDVQNMASFAFNDYFRKHGMTDDSCFFQNSAAITSLNPSFGNC 120

Query: 121 RIPASSLRNGNFSSQTPSVGLGPSEDISGSGRKSGRSWVWPMVIGYLFFRIV 171
           R P+S LRNGNFSSQT SVGLGPSED+SGS    GR+WVWPMVIGYL  RI+
Sbjct: 121 RFPSSVLRNGNFSSQTTSVGLGPSEDMSGSAHNLGRAWVWPMVIGYLLLRIM 172

BLAST of Cp4.1LG15g08460 vs. TAIR 10
Match: AT3G58100.1 (plasmodesmata callose-binding protein 5 )

HSP 1 Score: 142.1 bits (357), Expect = 4.4e-34
Identity = 76/152 (50.00%), Postives = 99/152 (65.13%), Query Frame = 0

Query: 10  LILLILGSSAAESRQPSTRDQSG---ATELWCVAKNNADDASLQSALDWACGAGGADCSP 69
           L L IL ++AA     +T    G     ELWCVAKNNA+D+SLQ+A++WACG GGADC P
Sbjct: 11  LRLSILAATAAMLMVITTAQIGGQVIQVELWCVAKNNAEDSSLQTAIEWACGQGGADCGP 70

Query: 70  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 129
           IQ GG C D ++VQ MASF FN+Y+ K+G  D++C F N AA+TSLNPS G C+ P+S  
Sbjct: 71  IQQGGPCNDPTDVQKMASFVFNNYYLKNGEEDEACNFNNNAALTSLNPSQGTCKYPSSKG 130

Query: 130 RNGNFSSQTPSVGLGPSEDISGSGRKSGRSWV 159
            N    +   S+G G + D+S  GR    SW+
Sbjct: 131 ANNGRLADDTSMGAGQA-DMSRGGRPISSSWM 161

BLAST of Cp4.1LG15g08460 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 102.1 bits (253), Expect = 5.0e-22
Identity = 53/101 (52.48%), Postives = 67/101 (66.34%), Query Frame = 0

Query: 37  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGM 96
           WC+AK NA   SLQ ALD+ACG GGADC  IQ G ACY+ + +++ ASFAFN Y++KH  
Sbjct: 148 WCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQKH-P 207

Query: 97  TDDSCFFQNAAAITSLNPSYGNCRIPASSLRNGNFSSQTPS 138
             DSC F  AA +TS +PS G+C   +SS   G  S+  PS
Sbjct: 208 GSDSCNFGGAAQLTSTDPSKGSCHFSSSS---GTVSTSPPS 244

BLAST of Cp4.1LG15g08460 vs. TAIR 10
Match: AT4G13600.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 1.5e-21
Identity = 60/143 (41.96%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 7   LFLLILLILGSSAAESRQPSTRDQSGATELWCVAKNNADDASLQSALDWACGAGGADCSP 66
           L  L+LL+  SSAA               +WCVA+ +    +LQ+ALD+AC A GADC+P
Sbjct: 46  LSFLLLLLFRSSAA---------------MWCVARFDVTSQALQAALDYAC-AAGADCAP 105

Query: 67  IQPGGACYDASNVQNMASFAFNDYFRKHGMTDDSCFFQNAAAITSLNPSYGNCRIPASSL 126
           IQP G C+  + VQ  AS+AFN YF++  M   SC F   + I   +PSYG+C  P +S+
Sbjct: 106 IQPNGLCFLPNTVQAHASYAFNSYFQRAAMAPGSCNFAGTSTIAKTDPSYGSCVYP-NSV 165

Query: 127 RNGNFSSQTPSVGLGPSEDISGS 150
           RN   S+ T +VG  PS  +  S
Sbjct: 166 RNAGGSASTTTVGGTPSTTVGNS 171

BLAST of Cp4.1LG15g08460 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.5e-21
Identity = 44/90 (48.89%), Postives = 62/90 (68.89%), Query Frame = 0

Query: 35  ELWCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRKH 94
           + +C+A +  D  +LQ+ALDWACG G ++CS IQPG +CY  +NV+  ASFAFN Y++K 
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 95  GMTDDSCFFQNAAAITSLNPSYGNCRIPAS 125
           G    SC F+  A IT+ +PS+G+C  P S
Sbjct: 439 GRASGSCDFKGVAMITTTDPSHGSCIFPGS 468

BLAST of Cp4.1LG15g08460 vs. TAIR 10
Match: AT2G05790.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.2e-21
Identity = 44/86 (51.16%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 37  WCVAKNNADDASLQSALDWACGAGGADCSPIQPGGACYDASNVQNMASFAFNDYFRKHGM 96
           WCVA  +A +  LQ  LD+ACG GGADC PIQPG  CY    ++  ASFAFN Y++K G 
Sbjct: 384 WCVANGDAGEERLQGGLDYACGEGGADCRPIQPGANCYSPDTLEAHASFAFNSYYQKKGR 443

Query: 97  TDDSCFFQNAAAITSLNPSYGNCRIP 123
              SC+F  AA + S  P YG C  P
Sbjct: 444 AGGSCYFGGAAYVVSQPPKYGRCEFP 469

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2K66.1e-3350.00PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Arabidopsis thaliana OX=3702 GN=PDCB5... [more]
O653993.5e-2048.89Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ... [more]
Q9M0695.0e-1936.42Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94CD83.3e-1842.02Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ... [more]
Q8VYE52.1e-1744.32Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
XP_023554742.14.78e-130100.00PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X2 [Cucurbita pepo subsp. p... [more]
XP_022952645.16.78e-13099.45PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita moschata][more]
XP_022969385.11.04e-12496.17PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like [Cucurbita maxima][more]
XP_023554741.12.46e-11399.38PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 [Cucurbita pepo subsp. p... [more]
KAG6572411.11.54e-10699.36PLASMODESMATA CALLOSE-BINDING PROTEIN 5, partial [Cucurbita argyrosperma subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1GMC73.28e-13099.45PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HZS55.03e-12596.17PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1JNE04.14e-9983.14PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1EY092.79e-9781.98PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like isoform X1 OS=Cucurbita moschata OX... [more]
A0A1S3BN123.15e-9681.98PLASMODESMATA CALLOSE-BINDING PROTEIN 5 OS=Cucumis melo OX=3656 GN=LOC103491346 ... [more]
Match NameE-valueIdentityDescription
AT3G58100.14.4e-3450.00plasmodesmata callose-binding protein 5 [more]
AT1G29380.15.0e-2252.48Carbohydrate-binding X8 domain superfamily protein [more]
AT4G13600.11.5e-2141.96Carbohydrate-binding X8 domain superfamily protein [more]
AT1G11820.22.5e-2148.89O-Glycosyl hydrolases family 17 protein [more]
AT2G05790.14.2e-2151.16O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 36..121
e-value: 3.6E-42
score: 156.1
IPR012946X8 domainPFAMPF07983X8coord: 36..107
e-value: 1.2E-19
score: 70.7
NoneNo IPR availableGENE3D1.20.58.1040coord: 31..133
e-value: 2.0E-11
score: 46.2
NoneNo IPR availablePANTHERPTHR31044:SF79BETA-1,3 GLUCANASEcoord: 28..136
IPR044788Carbohydrate-binding X8 domain-containing protein, plantPANTHERPTHR31044BETA-1,3 GLUCANASEcoord: 28..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g08460.1Cp4.1LG15g08460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009506 plasmodesma