Cp4.1LG15g08030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g08030
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 ubiquitin-protein ligase RMA1H1-like
Location	Cp4.1LG15: 8037660 .. 8041418 (-)
RNA-Seq Expression	Cp4.1LG15g08030
Synteny	Cp4.1LG15g08030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGGTGTTAAGAGTTGGCGCCTTTAAATCTCGGCGTCAACGTTACGTCTCTCTCATTTTTTATGTTCTTCAGTTTCTTCTTCTTCTTCTTCTTCGATCTCACATTTCGTTCTCGTTCTTTTTTTTTTTTATTGTTTCTGATAATTTTCCGTTGGAGCTCTGTGATTTTGCGGTACGAAGTTTCATATCCCTAAATTCTGCGTACTCCCTGTTACTGGCAACCTTCTTCTGGATTCCCGTGCCTCTCTGAATCGAAGCGACTAATTCCTGTTCGAAATTTGGTACGCTTTGAACTTGAGGATTTTGAATGTTGAACTTGTGTTTTCTTCTTCTGCGGAATAATTATCGGCTCATGTCTGATTCTTGACAGAATAGGGTTTTTTTCCTTTCGAATTGAAGCATTAGTTTTCATCTTAACGAATTTCTAACAAAATTTCATCGGAAGAAGATTGGATCAGTCATTTCGTGTTCTAATTGCATATTCACTTGATTTCTGTGTGATTAGCTTTTGAAATCCGTTTTAGATTGTTGGAAATATTTTAGAAAACTGGTTTAGATATGGATAAACATGTTTAAGAAGAGGTAATTTAGTTTAGAATCGTATGAGAGTGACTTCGAACAGAATTTTGGATAGCTATCCTACTTTTTTTATTCAAAATTTTTGTTGAATGTTCTAAGAGATTTCTTGTTGGAGTAGTTTTAAAATGTAGTATTTTTTAAAGATTGAACAAAATTCAAAAGAATTTTTTATAATTTATGATGAACAGTTCTAATCTTATGTCTTTATCAGCTGATTCATGCGCCTGTACTCAAGATTAGCTTGAGCCGACACTACCATGTTTGTTTAATTAATTTTCGGTATAGCTCAAGATTTATTTATTTTGGATCTGGATATGGATAGTTTCTAGCCCCAAAAAACTTTCCAGAAGGTCAACTTGCAAATAAGATTTAAATATCTGATTGGCCTGCAGCATTAACCAAGGGTTTCTCTTTATAATATTCTGAAAGCACATAACTCTACCTCTTATCTCCTTGCTCTTATCTTTCCTTGGGATTCAAGGTAATGATTTTTGTAATCTGAGAGAACCACAACAAGTTCATATCTGCATGTAAACATATATTTTCTCTGTTTCTTCAAAGATCTTGTGGTTGTTCGATCTTTCGATCCAATATTGATCTAAGTTTTTATGATTTGGTAGTTTAGGCTTCTCAGAAGGGTTGTGTTACAACAGAGCATTTGGAGATGGAGATTAACTCATTTGGAGAGAACAAGTCTTGGAAGTCTGTTTTGGATGCCATGGCTGACACTCATGGTGATGCCTCTGGGCTATTCGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGCCTTTACAAATGGCTTCACTTTCAGGACAGCTCTAAAGAAAAACATGGCCGGCGACTCCAGCAATGTCCAGTTTGTAAGGCAGAAGTTTCTGATGCTACTCTAGTGCCACTCTATGGCAAAGGTGGAACTCAAGACCCGTTCGAAAGCAAGAATTCGCAGCTTGGAATCGTCGTCCCTCGCAGGCCTAACGGTCCTGCTTGCTTTGAGTTACCAACGCCAACTTCTCACCCAACCTCTCACCCAGTTCCTCAATCTAGGCAAGGAAATTCCTCTAATCAATCAAACATTTGCTATGCTCAAGTGACAGGAGTGTTTGGAGAAGTGGTTTATGCAAGAATGTTCGGTGCAATAACGAATCTATATGCATATCCCAATGCATACCCTCTTGTGTGGAGCAGTAGCCCAAGGATCAGAAGGCATATTTTGCAGACTGATGAATCACTCAACAGAATCTGTATTTTTCTTTTCTGTTTCTTAGTTATCTGTCTTCTTTTGTTCTGAGCCATAGATTTTCCCTTTACTCTCTAATCCATTGTAAAAATTGAGTCAGAAGAACACTTAAATGATATGCAGATTGTGTTATGTAGGTGTGGCTGAGCGTTGGTGGTGATGTTGCCCCTTGGCTCGCCCTTATGTAGTTGATGTAAATAATAATTTTGTTTACCACCTCGTTTTATGGCCTTCTATTACTCTTTGTGAAATTTTACATCGGTTGGAGAGAAACAAAACATTCCTTATAAGAGTGTAGAAATCTCCCTCTAGCAAACGTGTTTTAAAACCTAGAAGTAGAAACTCAAAGAAGATAATATCTGCTGGCAGTGGGGTTGAGTTGTTACAAGTAATATTAGAGTCAATCACTAGGCGGTGTGCCAGCAAGGATATTGGGCCCCCAAGGAGGTGGATTGTGAGATCGCACATCGATTGAAGAGGGGAACGAAACATTCTTTATAAGAATGTGAAAACCTCTCCATAACAGCCACGTTTTAAAACCTTGAAGGAAAGCCTAGAAGAGAAAGCCCAAATAAGACAATATCGGCTAGCGGTGGACTCTTGGATTCGCCTATTCTACTAACAAAATAGAAAATGAAGAGGATTTGCAGTGGCAATCCAAATTTACATTTGAAATTCTTTAAGGACCCAAAACAAGTGGAATTTTACCAATCACGAGAAGAGCTGGTCTTGTGTTTCAACCATAGCCTCTGTCAACGATTTTATAGTGCTGAGACTTGCGATGAGCCTAAAAGAATTTTACCAATCACTACAAGTTGGTCTTAGTTCCCAGGATTTTGTGCACTTCTCCATCAACAGAAAAACCATCCATCTCCATCTGGTTTAGAATGTTGAGCACTGGTTTTCCACCTGCAATGGAAGTCCTCAGCGATCTGTAGTACATCTCCTTCGTTATCGATCCCGAGCTTCTGCATAGTTGTATCATTTCTTCCATTGTTTCTCCATCTCCTTGCTCTTCCAAGTAATTCAGACAGGCTTCCAAAATGTCTTGGTTTGGTTTCCAATCCTGCCTTCCAACTAACATAGCTCTCTTCAACATCTCAACTGCTTTGCTCATGAGCCCGTGTTCGACGTATCCCATAGCTAACACGCTCCACGTGCTAGCATATGGAGTTCTTCCCATCACTGCACGGTTAACGAGCGATTCCGCCTTATCAAAAAGACCTTTTCTGCAATAAGCAACCAGAAGTCGATTCAATACTCGAAAATCATAGAATGTGCACTTTGATTCCCACTCCTGGAAAATCCTTTCAGCCCCTTCAATATCATCAAGTTTCACTAGAGATGTGATCATTAAAGCATATGGCACATCTATTTGTTTAATTGTTGACTTGAACGTACTCCAAACCCTGTAAAGTTCGTCCTTACGACCTGCTCGTTCATAAAGCGACAGTAGAAACTCAAACGCCGATTTGTTCTTACGCGGTGGAATCTTCTCCTCCATTTTCTTCAGCATGGAGAGAGCCTCTGTTTCCAACCCAGCTGATAGATATCCACTAGCAGCAATTGAATAAATTCTCCAATCAGCTCTGAATTCAGAATTCTCCTCAATCCTTTTGAGGATCTTTTCCATACCAGAAATGTCTGCATTAGCAACATAAGCTGCACTAAGATTTCTAACAGTGTAGATATCTTGAGGTATTCCCTTCATTTCCATTTCTTGAATCAATAGATCAATCTTATGATGCTGCCCGACCTGAGCGTAGAGGTTAATTAGCACGTTATACGGAAACGACGTAGTAGCAAACCCCAAGTTTCTCATTTCTTGCATAATGGCTTCAGCTTTCTCAACTGATCTCTCTCGCACATAACTACAGAGAAGAGCGCCATAAGCATTAG

mRNA sequence

ATGACGGCTTCTCAGAAGGGTTGTGTTACAACAGAGCATTTGGAGATGGAGATTAACTCATTTGGAGAGAACAAGTCTTGGAAGTCTGTTTTGGATGCCATGGCTGACACTCATGGTGATGCCTCTGGGCTATTCGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGCCTTTACAAATGGCTTCACTTTCAGGACAGCTCTAAAGAAAAACATGGCCGGCGACTCCAGCAATGTCCAGTTTGTAAGGCAGAAGTTTCTGATGCTACTCTAGTGCCACTCTATGGCAAAGGTGGAACTCAAGACCCGTTCGAAAGCAAGAATTCGCAGCTTGGAATCGTCGTCCCTCGCAGGCCTAACGGTCCTGCTTGCTTTGAGTTACCAACGCCAACTTCTCACCCAACCTCTCACCCAGTTCCTCAATCTAGGCAAGGAAATTCCTCTAATCAATCAAACATTTGCTATGCTCAAGTGACAGGAGTGTTTGGAGAAGTGGTTTATGCAAGAATGTTCGGTGCAATAACGAATCTATATGCATATCCCAATGCATACCCTCTTGTGTGGAGCAGTAGCCCAAGGATCAGAAGGCATATTTTGCAGACTGATGAATCACTCAACAGAATCTGTATTTTTCTTTTCTGTTTCTTAGTGTGGCTGAGCGTTGGTGGTGATGTTGCCCCTTGGCTCGCCCTTATGACCCAAAACAAGTGGAATTTTACCAATCACGAGAAGAGCTGGTCTTGTGTTTCAACCATAGCCTCTGTCAACGATTTTATAGTGCTGAGACTTGCGATGAGCCTAAAAGAATTTTACCAATCACTACAAGTTGGTCTTAGTTCCCAGGATTTTGTGCACTTCTCCATCAACAGAAAAACCATCCATCTCCATCTGATCAATCTTATGATGCTGCCCGACCTGAGCGTAGAGCTTTCTCAACTGATCTCTCTCGCACATAACTACAGAGAAGAGCGCCATAAGCATTAG

Coding sequence (CDS)

ATGACGGCTTCTCAGAAGGGTTGTGTTACAACAGAGCATTTGGAGATGGAGATTAACTCATTTGGAGAGAACAAGTCTTGGAAGTCTGTTTTGGATGCCATGGCTGACACTCATGGTGATGCCTCTGGGCTATTCGACTGTAACATATGCTTAGAGACTGTGAAAGATCCTGTTGTTACACTTTGTGGCCATCTCTTTTGCTGGCCCTGCCTTTACAAATGGCTTCACTTTCAGGACAGCTCTAAAGAAAAACATGGCCGGCGACTCCAGCAATGTCCAGTTTGTAAGGCAGAAGTTTCTGATGCTACTCTAGTGCCACTCTATGGCAAAGGTGGAACTCAAGACCCGTTCGAAAGCAAGAATTCGCAGCTTGGAATCGTCGTCCCTCGCAGGCCTAACGGTCCTGCTTGCTTTGAGTTACCAACGCCAACTTCTCACCCAACCTCTCACCCAGTTCCTCAATCTAGGCAAGGAAATTCCTCTAATCAATCAAACATTTGCTATGCTCAAGTGACAGGAGTGTTTGGAGAAGTGGTTTATGCAAGAATGTTCGGTGCAATAACGAATCTATATGCATATCCCAATGCATACCCTCTTGTGTGGAGCAGTAGCCCAAGGATCAGAAGGCATATTTTGCAGACTGATGAATCACTCAACAGAATCTGTATTTTTCTTTTCTGTTTCTTAGTGTGGCTGAGCGTTGGTGGTGATGTTGCCCCTTGGCTCGCCCTTATGACCCAAAACAAGTGGAATTTTACCAATCACGAGAAGAGCTGGTCTTGTGTTTCAACCATAGCCTCTGTCAACGATTTTATAGTGCTGAGACTTGCGATGAGCCTAAAAGAATTTTACCAATCACTACAAGTTGGTCTTAGTTCCCAGGATTTTGTGCACTTCTCCATCAACAGAAAAACCATCCATCTCCATCTGATCAATCTTATGATGCTGCCCGACCTGAGCGTAGAGCTTTCTCAACTGATCTCTCTCGCACATAACTACAGAGAAGAGCGCCATAAGCATTAG

Protein sequence

MTASQKGCVTTEHLEMEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLVWLSVGGDVAPWLALMTQNKWNFTNHEKSWSCVSTIASVNDFIVLRLAMSLKEFYQSLQVGLSSQDFVHFSINRKTIHLHLINLMMLPDLSVELSQLISLAHNYREERHKH

Homology

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BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.9e-51
Identity = 107/226 (47.35%), Postives = 139/226 (61.50%), Query Frame = 0


Query: 27  WKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHG 86
           WKS+ D + +   + SG FDCNICL+ V +PV+TLCGHL+CWPC+YKW++FQ  S E   
Sbjct: 24  WKSMNDEVEE---NISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSD 83

Query: 87  RRLQQCPVCKAEVSDATLVPLYGKGG-TQDPFESKNSQLGIVVPRRPNGPAC---FELPT 146
           ++  QCPVCKAEVS+ TL+PLYG+GG +  P E K   LGIV+P+RP  P C   F LPT
Sbjct: 84  QQQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPT 143

Query: 147 PTSHPT-----------SHPVPQSRQGNSSNQSNICYAQVT------GVFGEVVYARMFG 206
             S+P+           S     + QG+  +   +     T       + GEV YAR+FG
Sbjct: 144 TDSNPSQLLQRRGYQQQSQTRQPAYQGSYMSSPMLSPGGATANMLQHSMIGEVAYARIFG 203

Query: 207 -AITNLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 231
            + T +Y YPN+Y L  SSSPR+RR + Q D SL RIC FLFC  V
Sbjct: 204 NSSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRICFFLFCCFV 246

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.4e-38
Identity = 90/205 (43.90%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 45  FDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATL 104
           FDCNICL++V++PVVTLCGHLFCWPC++KWL  Q  S     +R +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTS--------------- 164
           VPLYG+G      E KNS     VP+RP GP  + L  P S   S               
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPV-YRLEMPNSPYASTDLRLSQRVHFNSPQ 165

Query: 165 ---HPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGA-ITNLYAYPNAYPLVWSSSP 224
              +PV      NS + S +    V  + GE+V  R+FG  + + +AYP+ Y L  +S P
Sbjct: 166 EGYYPVSGVMSSNSLSYSAV-LDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGP 225

Query: 225 RIRRHILQTDESLNRICIFLFCFLV 231
           R+RR I+Q D+SL RI  F  C +V
Sbjct: 226 RMRRRIMQADKSLGRIFFFFMCCVV 243

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.0e-33
Identity = 79/196 (40.31%), Postives = 112/196 (57.14%), Query Frame = 0


Query: 40  DASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEK-----HGRRLQQCPV 99
           D+ G FDCNICL+ V+DPVVTLCGHLFCWPC++KW +  ++S+++     H R   +CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 100 CKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTSHPVPQ 159
           CK++VS+ATLVP+YG+G        K  Q G  VP RP GP          +       +
Sbjct: 74  CKSDVSEATLVPIYGRG-------QKAPQSGSNVPSRPTGPV---------YDLRGVGQR 133

Query: 160 SRQGNSSNQSNICYAQVTGVFGEVVYARMFG-AITNLYAYPNAYPLVWSSSPRIRRHILQ 219
             +G S          V GV  E+VY R+FG + +N+  Y +        + R RR  +Q
Sbjct: 134 LGEGESQRYMYRMPDPVMGVVCEMVYRRLFGESSSNMAPYRD-------MNVRSRRRAMQ 186

Query: 220 TDESLNRICIFLFCFL 230
            +ESL+R+ +FL CF+
Sbjct: 194 AEESLSRVYLFLLCFM 186

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-32
Identity = 87/222 (39.19%), Postives = 115/222 (51.80%), Query Frame = 0


Query: 35  ADTHGDA--SGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQC 94
           A T G A  SG FDCNICL+T  DPVVTLCGHLFCWPC+YKWLH Q SS     +    C
Sbjct: 30  APTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSV-DQHQNNC 89

Query: 95  PVCKAEVSDATLVPLYGKG--GTQDPFESKNSQ-LGIVVPRRPNGPACFELPT------- 154
           PVCK+ ++  +LVPLYG+G       F SK    L   +PRRP  P+    P        
Sbjct: 90  PVCKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP-APSALRNPITSASSLN 149

Query: 155 -----PTSHPTSHPVPQSRQGNSSNQS---------NICYAQVTGVFGEVVYARMFGAIT 214
                 T  P+ H    S +G ++ +S         +  Y  V G+FG++VY R+FG  T
Sbjct: 150 PSLQHQTLSPSFHNHQYSPRGFTTTESTDLANAVMMSFLY-PVIGMFGDLVYTRIFGTFT 209

Query: 215 NLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 231
           N  A P              + ++Q ++SLNR+ IF  C ++
Sbjct: 210 NTIAQPYQ-----------SQRMMQREKSLNRVSIFFLCCII 237

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.5e-19
Identity = 47/102 (46.08%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 35  ADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQCPV 94
           A   G     F+CNICL+T KD V++LCGHLFCWPCL++WL  + +         Q CPV
Sbjct: 27  AGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNR--------QVCPV 86

Query: 95  CKAEVSDATLVPLYGKG--GTQDPFESKNSQLGIVVPRRPNG 135
           CKA +S   ++PLYG+G  G QDP E          P RP G
Sbjct: 87  CKAGISRDKVIPLYGRGSTGQQDPREK--------TPPRPQG 112

[Close]

BLAST of Cp4.1LG15g08030 vs. NCBI nr
Match: XP_023554609.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023554610.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023554611.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_023554612.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >KAG7011971.1 E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461 bits (1187), Expect = 4.16e-162
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
           ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV
Sbjct: 181 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 215

[Close]

BLAST of Cp4.1LG15g08030 vs. NCBI nr
Match: XP_022969255.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita maxima] >XP_022969256.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita maxima] >XP_022969257.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita maxima] >XP_022969258.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita maxima])

HSP 1 Score: 452 bits (1163), Expect = 1.88e-158
Identity = 210/215 (97.67%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSW SV+DAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWTSVVDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
            CFELP P SHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 VCFELPKPASHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV
Sbjct: 181 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 215

[Close]

BLAST of Cp4.1LG15g08030 vs. NCBI nr
Match: XP_022952773.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022952774.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022952775.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022952776.1 E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata])

HSP 1 Score: 451 bits (1159), Expect = 7.65e-158
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRP+GP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPSGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
            CF+LP P SHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 VCFDLPKPASHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AYPLVWS SPRIRRHILQTDESLNRICIFLFCFLV
Sbjct: 181 AYPLVWSRSPRIRRHILQTDESLNRICIFLFCFLV 215

[Close]

BLAST of Cp4.1LG15g08030 vs. NCBI nr
Match: KAG6572360.1 (E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415 bits (1067), Expect = 3.63e-144
Identity = 199/230 (86.52%), Postives = 199/230 (86.52%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
           ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLVWLSVGGDVAPWLALM 245
           AYPL W                              LSVGGDVAPWLALM
Sbjct: 181 AYPLAW------------------------------LSVGGDVAPWLALM 200

[Close]

BLAST of Cp4.1LG15g08030 vs. NCBI nr
Match: XP_038888270.1 (E3 ubiquitin-protein ligase RMA1H1 [Benincasa hispida])

HSP 1 Score: 393 bits (1010), Expect = 6.68e-135
Identity = 187/225 (83.11%), Postives = 201/225 (89.33%), Query Frame = 0


Query: 12  EHLEMEINSFGENKS----WKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFC 71
           E   ++INSFGENKS    WKSVLDAMA+TH DASGLFDCNICLETVKDPVVTLCGHLFC
Sbjct: 8   EEASIQINSFGENKSSLENWKSVLDAMAETHCDASGLFDCNICLETVKDPVVTLCGHLFC 67

Query: 72  WPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIV 131
           WPC+YKWLH+QDSS EK  RRL QCPVCKAEVSDATLVPLYGKG T+D FESKN+QLGIV
Sbjct: 68  WPCIYKWLHYQDSSLEKQARRLPQCPVCKAEVSDATLVPLYGKGKTRDSFESKNTQLGIV 127

Query: 132 VPRRPNGPACFELPTPTSHPTSHPVPQSRQGNS--SNQSNICYAQVTGVFGEVVYARMFG 191
           VPRRP+GPACFE P PTSHPTSH VPQ RQGNS  S+QSN+ YA++TG FGEVVYARM G
Sbjct: 128 VPRRPHGPACFESPRPTSHPTSHTVPQYRQGNSDSSDQSNVYYAEMTGAFGEVVYARMSG 187

Query: 192 AITNLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AITNLYAYPN+YPLVWSSSPRIRRHILQTDESLNRICIFLFC L+
Sbjct: 188 AITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCLI 232

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL
Match: A0A6J1HXA9 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111468313 PE=4 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 9.11e-159
Identity = 210/215 (97.67%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSW SV+DAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWTSVVDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
            CFELP P SHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 VCFELPKPASHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV
Sbjct: 181 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 215

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL
Match: A0A6J1GLI9 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC111455370 PE=4 SV=1)

HSP 1 Score: 451 bits (1159), Expect = 3.70e-158
Identity = 209/215 (97.21%), Postives = 211/215 (98.14%), Query Frame = 0


Query: 16  MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 75
           MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL
Sbjct: 1   MEINSFGENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWL 60

Query: 76  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGP 135
           HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRP+GP
Sbjct: 61  HFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPSGP 120

Query: 136 ACFELPTPTSHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 195
            CF+LP P SHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN
Sbjct: 121 VCFDLPKPASHPTSHPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAITNLYAYPN 180

Query: 196 AYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           AYPLVWS SPRIRRHILQTDESLNRICIFLFCFLV
Sbjct: 181 AYPLVWSRSPRIRRHILQTDESLNRICIFLFCFLV 215

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL
Match: A0A1S3C1W7 (E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103495518 PE=4 SV=1)

HSP 1 Score: 387 bits (995), Expect = 6.40e-133
Identity = 187/226 (82.74%), Postives = 199/226 (88.05%), Query Frame = 0


Query: 12  EHLEMEINSFGENKS----WKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFC 71
           E   ++INSFGENKS    WKSVLDAMA+TH DASGLFDCNICLETVKDPVVTLCGHLFC
Sbjct: 8   EEASIQINSFGENKSSLENWKSVLDAMAETHCDASGLFDCNICLETVKDPVVTLCGHLFC 67

Query: 72  WPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIV 131
           WPC+YKW+HFQDSS EK  RRL QCPVCKAEVSDATLVPLYGKG TQ+P ESKN QLGIV
Sbjct: 68  WPCIYKWIHFQDSSLEKQARRLPQCPVCKAEVSDATLVPLYGKGETQNPLESKNPQLGIV 127

Query: 132 VPRRPNGPACFELPTPTSHPTSHPV-PQSRQGNS--SNQSNICYAQVTGVFGEVVYARMF 191
           VPRRP GPACFE P PTSHPTSH V PQ R+G+S  S+QSN+ YAQVTG FGEVVYARM 
Sbjct: 128 VPRRPQGPACFESPRPTSHPTSHTVGPQFREGSSDSSDQSNVYYAQVTGAFGEVVYARMS 187

Query: 192 GAITNLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           GAITNLYAYPN+YPLVWSSSPRIRRHILQTDESLNRICIFLFC L+
Sbjct: 188 GAITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCLI 233

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL
Match: A0A5A7SLT9 (E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003470 PE=4 SV=1)

HSP 1 Score: 387 bits (995), Expect = 6.40e-133
Identity = 187/226 (82.74%), Postives = 199/226 (88.05%), Query Frame = 0


Query: 12  EHLEMEINSFGENKS----WKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFC 71
           E   ++INSFGENKS    WKSVLDAMA+TH DASGLFDCNICLETVKDPVVTLCGHLFC
Sbjct: 8   EEASIQINSFGENKSSLENWKSVLDAMAETHCDASGLFDCNICLETVKDPVVTLCGHLFC 67

Query: 72  WPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIV 131
           WPC+YKW+HFQDSS EK  RRL QCPVCKAEVSDATLVPLYGKG TQ+P ESKN QLGIV
Sbjct: 68  WPCIYKWIHFQDSSLEKQARRLPQCPVCKAEVSDATLVPLYGKGETQNPLESKNPQLGIV 127

Query: 132 VPRRPNGPACFELPTPTSHPTSHPV-PQSRQGNS--SNQSNICYAQVTGVFGEVVYARMF 191
           VPRRP GPACFE P PTSHPTSH V PQ R+G+S  S+QSN+ YAQVTG FGEVVYARM 
Sbjct: 128 VPRRPQGPACFESPRPTSHPTSHTVGPQFREGSSDSSDQSNVYYAQVTGAFGEVVYARMS 187

Query: 192 GAITNLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 230
           GAITNLYAYPN+YPLVWSSSPRIRRHILQTDESLNRICIFLFC L+
Sbjct: 188 GAITNLYAYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFCCLI 233

[Close]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL
Match: A0A6J1ERI0 (E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437136 PE=4 SV=1)

HSP 1 Score: 379 bits (972), Expect = 1.68e-129
Identity = 183/219 (83.56%), Postives = 193/219 (88.13%), Query Frame = 0


Query: 17  EINSFGENKS----WKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLY 76
           EINSFGENKS    WKS LDAMA+T  D+SGLFDCNICLETVKDPVVTLCGHLFCWPC+Y
Sbjct: 9   EINSFGENKSSVENWKSALDAMAETQCDSSGLFDCNICLETVKDPVVTLCGHLFCWPCIY 68

Query: 77  KWLHFQDSSKEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRP 136
           KWLHFQDSS EK G+RLQQCPVCKAEVSDATLVPLYGKG TQDPFESKNSQLGIVVPRRP
Sbjct: 69  KWLHFQDSSTEKQGQRLQQCPVCKAEVSDATLVPLYGKGETQDPFESKNSQLGIVVPRRP 128

Query: 137 NGPACFELPTPTSHPTSHPVP----QSRQGNSSNQSNICYAQVTGVFGEVVYARMFGAIT 196
            GPACFE P  TSHP  HP P    Q +QGN S+QSNI YA+  GVFGE+VYARMFGAIT
Sbjct: 129 QGPACFESPRATSHPHPHPHPHPVLQFQQGNLSDQSNIYYAE--GVFGEMVYARMFGAIT 188

Query: 197 NLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFC 227
           NLY+YPN+YPLVWSSSPRIRRHILQTDESLNRICIFLFC
Sbjct: 189 NLYSYPNSYPLVWSSSPRIRRHILQTDESLNRICIFLFC 225

[Close]

BLAST of Cp4.1LG15g08030 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 9.9e-40
Identity = 90/205 (43.90%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 45  FDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATL 104
           FDCNICL++V++PVVTLCGHLFCWPC++KWL  Q  S     +R +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTS--------------- 164
           VPLYG+G      E KNS     VP+RP GP  + L  P S   S               
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPV-YRLEMPNSPYASTDLRLSQRVHFNSPQ 165

Query: 165 ---HPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGA-ITNLYAYPNAYPLVWSSSP 224
              +PV      NS + S +    V  + GE+V  R+FG  + + +AYP+ Y L  +S P
Sbjct: 166 EGYYPVSGVMSSNSLSYSAV-LDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGP 225

Query: 225 RIRRHILQTDESLNRICIFLFCFLV 231
           R+RR I+Q D+SL RI  F  C +V
Sbjct: 226 RMRRRIMQADKSLGRIFFFFMCCVV 243

[Close]

BLAST of Cp4.1LG15g08030 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 161.8 bits (408), Expect = 9.9e-40
Identity = 90/205 (43.90%), Postives = 119/205 (58.05%), Query Frame = 0


Query: 45  FDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQCPVCKAEVSDATL 104
           FDCNICL++V++PVVTLCGHLFCWPC++KWL  Q  S     +R +QCPVCK++VS +TL
Sbjct: 46  FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRHRQCPVCKSKVSHSTL 105

Query: 105 VPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTS--------------- 164
           VPLYG+G      E KNS     VP+RP GP  + L  P S   S               
Sbjct: 106 VPLYGRGRCTTQEEGKNS-----VPKRPVGPV-YRLEMPNSPYASTDLRLSQRVHFNSPQ 165

Query: 165 ---HPVPQSRQGNSSNQSNICYAQVTGVFGEVVYARMFGA-ITNLYAYPNAYPLVWSSSP 224
              +PV      NS + S +    V  + GE+V  R+FG  + + +AYP+ Y L  +S P
Sbjct: 166 EGYYPVSGVMSSNSLSYSAV-LDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGP 225

Query: 225 RIRRHILQTDESLNRICIFLFCFLV 231
           R+RR I+Q D+SL RI  F  C +V
Sbjct: 226 RMRRRIMQADKSLGRIFFFFMCCVV 243

[Close]

BLAST of Cp4.1LG15g08030 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 144.1 bits (362), Expect = 2.1e-34
Identity = 79/196 (40.31%), Postives = 112/196 (57.14%), Query Frame = 0


Query: 40  DASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEK-----HGRRLQQCPV 99
           D+ G FDCNICL+ V+DPVVTLCGHLFCWPC++KW +  ++S+++     H R   +CPV
Sbjct: 14  DSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPPKCPV 73

Query: 100 CKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNGPACFELPTPTSHPTSHPVPQ 159
           CK++VS+ATLVP+YG+G        K  Q G  VP RP GP          +       +
Sbjct: 74  CKSDVSEATLVPIYGRG-------QKAPQSGSNVPSRPTGPV---------YDLRGVGQR 133

Query: 160 SRQGNSSNQSNICYAQVTGVFGEVVYARMFG-AITNLYAYPNAYPLVWSSSPRIRRHILQ 219
             +G S          V GV  E+VY R+FG + +N+  Y +        + R RR  +Q
Sbjct: 134 LGEGESQRYMYRMPDPVMGVVCEMVYRRLFGESSSNMAPYRD-------MNVRSRRRAMQ 186

Query: 220 TDESLNRICIFLFCFL 230
            +ESL+R+ +FL CF+
Sbjct: 194 AEESLSRVYLFLLCFM 186

[Close]

BLAST of Cp4.1LG15g08030 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 141.0 bits (354), Expect = 1.8e-33
Identity = 87/222 (39.19%), Postives = 115/222 (51.80%), Query Frame = 0


Query: 35  ADTHGDA--SGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSSKEKHGRRLQQC 94
           A T G A  SG FDCNICL+T  DPVVTLCGHLFCWPC+YKWLH Q SS     +    C
Sbjct: 30  APTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSV-DQHQNNC 89

Query: 95  PVCKAEVSDATLVPLYGKG--GTQDPFESKNSQ-LGIVVPRRPNGPACFELPT------- 154
           PVCK+ ++  +LVPLYG+G       F SK    L   +PRRP  P+    P        
Sbjct: 90  PVCKSNITITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRP-APSALRNPITSASSLN 149

Query: 155 -----PTSHPTSHPVPQSRQGNSSNQS---------NICYAQVTGVFGEVVYARMFGAIT 214
                 T  P+ H    S +G ++ +S         +  Y  V G+FG++VY R+FG  T
Sbjct: 150 PSLQHQTLSPSFHNHQYSPRGFTTTESTDLANAVMMSFLY-PVIGMFGDLVYTRIFGTFT 209

Query: 215 NLYAYPNAYPLVWSSSPRIRRHILQTDESLNRICIFLFCFLV 231
           N  A P              + ++Q ++SLNR+ IF  C ++
Sbjct: 210 NTIAQPYQ-----------SQRMMQREKSLNRVSIFFLCCII 237

[Close]

BLAST of Cp4.1LG15g08030 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 2.5e-27
Identity = 58/113 (51.33%), Postives = 70/113 (61.95%), Query Frame = 0


Query: 22  GENKSWKSVLDAMADTHGDASGLFDCNICLETVKDPVVTLCGHLFCWPCLYKWLHFQDSS 81
           GE+ +  S  D+  DT+ D  G F+CNIC E  +DP+VTLCGHLFCWPCLY+WLH     
Sbjct: 4   GESSTSTSYSDSNNDTN-DQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLH----- 63

Query: 82  KEKHGRRLQQCPVCKAEVSDATLVPLYGKGGTQDPFESKNSQLGIVVPRRPNG 135
              H    Q+CPVCKA V D  LVPLYG+G  Q    SK    G+ +P RP G
Sbjct: 64  ---HHSHSQECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYP-GLRIPNRPTG 106

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g08030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6R567	1.9e-51	47.35	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	1.4e-38	43.90	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	3.0e-33	40.31	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q8GUK7	2.5e-32	39.19	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
Q96GF1	2.5e-19	46.08	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]

BLAST of Cp4.1LG15g08030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554609.1	4.16e-162	100.00	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo] >XP_0235546...	[more]
XP_022969255.1	1.88e-158	97.67	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita maxima] >XP_022969256.1 E3 ub...	[more]
XP_022952773.1	7.65e-158	97.21	E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita moschata] >XP_022952774.1 E3 ...	[more]
KAG6572360.1	3.63e-144	86.52	E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_038888270.1	6.68e-135	83.11	E3 ubiquitin-protein ligase RMA1H1 [Benincasa hispida]	[more]

BLAST of Cp4.1LG15g08030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HXA9	9.11e-159	97.67	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111468...	[more]
A0A6J1GLI9	3.70e-158	97.21	E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A1S3C1W7	6.40e-133	82.74	E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103495518 PE=4 ...	[more]
A0A5A7SLT9	6.40e-133	82.74	E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1ERI0	1.68e-129	83.56	E3 ubiquitin-protein ligase RMA1H1-like isoform X1 OS=Cucurbita moschata OX=3662...	[more]

BLAST of Cp4.1LG15g08030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03510.1	9.9e-40	43.90	RING membrane-anchor 1	[more]
AT4G03510.2	9.9e-40	43.90	RING membrane-anchor 1	[more]
AT4G28270.1	2.1e-34	40.31	RING membrane-anchor 2	[more]
AT4G27470.1	1.8e-33	39.19	RING membrane-anchor 3	[more]
AT2G23780.1	2.5e-27	51.33	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 47..95 e-value: 5.1E-10 score: 49.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 47..96 score: 13.049892
IPR027370	RING-type zinc-finger, LisH dimerisation motif	PFAM	PF13445	zf-RING_UBOX	coord: 47..93 e-value: 4.8E-9 score: 36.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 31..109 e-value: 3.9E-18 score: 66.6
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 22..230
None	No IPR available	PANTHER	PTHR12313:SF59	E3 UBIQUITIN-PROTEIN LIGASE RMA1	coord: 22..230
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 45..97 e-value: 1.44971E-24 score: 92.3671
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 38..101
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 62..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g08030.1	Cp4.1LG15g08030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity