Cp4.1LG15g07320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g07320
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RNase III domain-containing protein
Location	Cp4.1LG15: 7685723 .. 7687793 (+)
RNA-Seq Expression	Cp4.1LG15g07320
Synteny	Cp4.1LG15g07320

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAGGGGCCGAAGGACGAGAGATATTTCATACAGATTAACCAGAGAGGGAAAAAGAATACTCTTAGGACCGAATTAAATTAAAATTTCTTTACAAATCCCTAAATTCCTTTATGGAAGGAGGAGGAGACGGCTGCGATTTGTTGGAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCTGCGAGTCTGGACGGAATGGAAGCTATTTTGAACTACGAGTTTCGAGACAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCCGGTTCACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTTGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAGTAAGTACCGATTGCTTTGATTTTTCATCGTTTTCATCGACTTCTCTCAACTTCCTCTGTTTTCTTCTGCTTGTTTCATAATTATGATCATTAACTGTTAACCAACCCACTAATTACGATATTTCTTTTCTCTTTCGCGTTATTCAATTTTATTCCTTTTTAAAAAATTTCAATTCTTCAATTTTTGTTTCAAAATTTAAAATCTTATATATATATATATATATTTTAATTGCCTAAATTTAATTCAAACAATTTGCGTATTTGATTAATAATTTATGATTTATTAAAATTATATTAGATATTTGAAAATTTAATTAATTCGATTTCAACCATAAATTTTTTCCATAACCATTATTTTTTAAATATAATTTCGTTCTTTTTTTCCTTTTTCAGTAAGGATATTTTTTTTTTCTTTTCCAAAATCTTTATTTTATATTTTTTTTTGTGCAATTTTAGTAGTAAAATCGGAGAGAAATATATTCCGTCTCTTTCCTAAAATTGCTTTTGAATTTTTTTTCTTTTTCGAAAAACCCTCTTATGTTTGATTTAAATTAGTCAATATGAAGGTAAACAATTTTTTTAATTGATTCAACGAAATATAAAGTGACAGTAATAGTAATTAATTAATTAATTAAAAAGGCGTGATAATTCATGGAAATTAATTGTGGTTTAGGGCATGCTCTTACATTTATTCTTTTGAAATATTTGAATCTTTTAAATTAATTAATAAGACGTGAAAATTAAATGTTTGAAATCCTATTTCTTCTATTATTTTATTAAAATTAAATTTTAAAAATCATTTGTTAAAAAAAAAAAAAACTATTATATGTTAGAATAATTTAGTTTTTTCTTTTTTGGGAATAATTTAAATTTTGTTTGTGTGACAACAGATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTAGAATCATGCATTGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGGTAAATAGATTTAAAGAAGAAGGATGAAAATTTTGAATTATAATGGTAAGTAGTTTTGATTGAAGTATAGGATGGTTTGATTTGTGTAGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACGGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAATCTATTCCTAATTATACTTACTCCCTTTTCTTTTCTTTTTTATTATTTTTTTTAATTTTTAATTAATCATTCTTTTATCTCTTTCAAATGAGTGTTAATTCCAAAGTAATTTCTAGAGTTGTTATTATATTTTTTTTTTTTGAATAAAGTATAAAGTTTTGATATATATATATATATATAT

mRNA sequence

TAGGGGCCGAAGGACGAGAGATATTTCATACAGATTAACCAGAGAGGGAAAAAGAATACTCTTAGGACCGAATTAAATTAAAATTTCTTTACAAATCCCTAAATTCCTTTATGGAAGGAGGAGGAGACGGCTGCGATTTGTTGGAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCTGCGAGTCTGGACGGAATGGAAGCTATTTTGAACTACGAGTTTCGAGACAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCCGGTTCACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTTGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTAGAATCATGCATTGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACGGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAATCTATTCCTAATTATACTTACTCCCTTTTCTTTTCTTTTTTATTATTTTTTTTAATTTTTAATTAATCATTCTTTTATCTCTTTCAAATGAGTGTTAATTCCAAAGTAATTTCTAGAGTTGTTATTATATTTTTTTTTTTTGAATAAAGTATAAAGTTTTGATATATATATATATATATAT

Coding sequence (CDS)

ATGGAAGGAGGAGGAGACGGCTGCGATTTGTTGGAGCAGGAAGGCAGAGGCGGCGAGTCAACGGCTGCGAGTCTGGACGGAATGGAAGCTATTTTGAACTACGAGTTTCGAGACAAGAGATTGCTGGAAGAGGCTTTCACAGACGCGTCGTATTCGCCGGAAAATTGCTCGTCGTTCGAGCGATTGGAGTACGTAGGCGATTCGGTGTTGAATTTTCTAATAACCAGAGAGCAATACTTCTCGAATCCGAATCTGTCGCCCGGTTCACTGACTCGTCTCCGAGCGGCGAATGTGGACACCGAAAAGCTTGCTCGTGTTGCGATTACACATGGATTTCATCGGTATCTTCGCCACAACAAGGCTGATCTTGATCAGAAAATAGAAGAGTTTAGTAAGAATATAGAGGAGTACCCATTGCATTCGAACGGACTGATTAAAGCTCCAAAGATTCTAGCGGACATAGTAGAATCATGCATTGGAGCAGTTTTCACAGATAGTAACTCGATCGACACAGTTTGGACGATATTCAAGGGTCTAATGGAGCCATTGATAACATTGAAGACCATGAAAAGGCACCCGATGACGGAGCTGACTGAAATGTGTCAAAAAAGGAACCTGAAGTGGGAATTCGTGGACTTGTGGGAAGAAAGAAAGGAGATCCACGTTTTGATAGACAAGCAGCTGGTTGGAAAAGGAAGCTATGGGAAGAAGCTCATTGCACAAAATCGAGCTGCAAAAGATGCTTTGGATAACTTCCACACAATTCTTGGCCAATTTAATGAACCTTGTCAACCTATGTAA

Protein sequence

MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPCQPM

Homology

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 3.3e-59
Identity = 119/236 (50.42%), Postives = 164/236 (69.49%), Query Frame = 0


Query: 28  MEAILNYEFRDKRLLEEAFTDAS--YSPENCSSFERLEYVGDSVLNFLITREQYFSNPNL 87
           +E++L YEFRDK L+EEA T  S  Y      ++ERLEY+GD+VL  +++RE + +   L
Sbjct: 42  VESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSREVFLTYGQL 101

Query: 88  SPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNGLI 147
            PG LTRLRAANVD EKLARVA+ HG H +LRH   +LD +I +F + +  YP+HSNGL+
Sbjct: 102 QPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPIHSNGLL 161

Query: 148 KAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQKR 207
             PK+L D+VES IGA++ DSN + + VW +F+ L +PLI+L+T+ +HP++EL E CQK 
Sbjct: 162 DPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVSELFEFCQKT 221

Query: 208 NLKWEFV-DLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRAAKDALDNFHTILGQ 259
               + V D W++   + VLID ++VG+ +Y  KK IAQNRAAK ALD     LGQ
Sbjct: 222 RRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQNRAAKAALDKLKETLGQ 277

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 171.4 bits (433), Expect = 1.4e-41
Identity = 103/241 (42.74%), Postives = 144/241 (59.75%), Query Frame = 0


Query: 19   ESTAASLD--GMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1551 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1610

Query: 79   EQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1611 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1670

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1671 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1730

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1731 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.4e-41
Identity = 95/237 (40.08%), Postives = 150/237 (63.29%), Query Frame = 0


Query: 20  STAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79
           S   S++ +E ILNY+F +K LL+EA T  S +  +  S+ERLE++GDS +   I+   Y
Sbjct: 55  SEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYLY 114

Query: 80  FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNI---EE 139
            + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EFS+ +   ++
Sbjct: 115 LTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDD 174

Query: 140 YPLHSNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKR--HP 199
             +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++   P
Sbjct: 175 LSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQP 234

Query: 200 MTELTEMCQKRNLKWEFVDLWEERKEIHVL-IDKQLVGKGSYGKKLIAQNRAAKDAL 250
           ++ L ++C K   + +  +  +    I V+ +D +L+  G    K IA+  AAK+AL
Sbjct: 235 VSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEAL 289

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 3.4e-40
Identity = 93/231 (40.26%), Postives = 142/231 (61.47%), Query Frame = 0


Query: 28  MEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSP 87
           +E +L Y+FRD RLLEEA T  S++ ++  S++RLE+VGDS L    +   Y +NP L P
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGP 99

Query: 88  GSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYP-------LH 147
           G L+ LRAAN+ TEKLARVA+ H  +  LR N   LD  + +F + +++ P        +
Sbjct: 100 GPLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPY 159

Query: 148 SNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTE 207
              ++KAPK+LADIVE+   AV+ D    ++ +W + + L EP+IT +T+   P+T L E
Sbjct: 160 GGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAETIDEQPVTMLHE 219

Query: 208 MCQKRNLKWEFVDLWEE--RKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 249
           +CQK     +F   W++     ++V +  +LVG GS  +K+IA+  AA+DA
Sbjct: 220 LCQKHGKMAQF-KTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDA 268

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.9e-39
Identity = 96/234 (41.03%), Postives = 140/234 (59.83%), Query Frame = 0


Query: 24   SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83
            + D ++ +L  EF++K LL EA T AS      S ++RLE+VGD+VL+ LITR  +F+  
Sbjct: 1537 NFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYT 1596

Query: 84   NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPL---- 143
            +L PG LT LRAA V+ E  ARVA+ H  H +LRH  + L+ +I EF K+++E  L    
Sbjct: 1597 DLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGF 1656

Query: 144  HSNGL--IKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTE 203
            +S GL   KAPK+L DIVES  GA+F DS      VW +F+ L+ P++T +T+  HP+ E
Sbjct: 1657 NSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPMHPVRE 1716

Query: 204  LTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDAL 250
            L E CQ++    E+          + V +D   +G     +K +AQ  AA++AL
Sbjct: 1717 LQERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNAL 1770

[Close]

BLAST of Cp4.1LG15g07320 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 538 bits (1385), Expect = 7.71e-193
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. NCBI nr
Match: XP_022952611.1 (ribonuclease 3-like protein 3 [Cucurbita moschata])

HSP 1 Score: 528 bits (1360), Expect = 5.00e-189
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLL+QEGRGGESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 528 bits (1360), Expect = 5.00e-189
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLL+QEGR GESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGVDLLKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNG+IKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. NCBI nr
Match: KAG7011897.1 (Ribonuclease 3-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 527 bits (1358), Expect = 1.01e-188
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEG GDG DLL+QEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGRGDGGDLLKQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. NCBI nr
Match: KAG6572281.1 (Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524 bits (1350), Expect = 1.67e-187
Identity = 261/266 (98.12%), Postives = 263/266 (98.87%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEG GD  DLL+QEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGRGDRGDLLKQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 528 bits (1360), Expect = 2.42e-189
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLL+QEGR GESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGVDLLKQEGRVGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNG+IKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGMIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 528 bits (1360), Expect = 2.42e-189
Identity = 262/266 (98.50%), Postives = 264/266 (99.25%), Query Frame = 0


Query: 1   MEGGGDGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFE 60
           MEGGGDG DLL+QEGRGGESTAASLDGMEAIL YEFRDKRLLEEAFTDASYSPENCSSFE
Sbjct: 1   MEGGGDGGDLLKQEGRGGESTAASLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFE 60

Query: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120
           RLEYVGDSVLNFLITREQYFSNPNLSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK
Sbjct: 61  RLEYVGDSVLNFLITREQYFSNPNLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNK 120

Query: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180
           ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM
Sbjct: 121 ADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLM 180

Query: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240
           EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA
Sbjct: 181 EPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIA 240

Query: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266
           QNRAAKDALDNFHTILGQFNEPCQPM
Sbjct: 241 QNRAAKDALDNFHTILGQFNEPCQPM 266

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 433 bits (1113), Expect = 1.47e-151
Identity = 224/275 (81.45%), Postives = 238/275 (86.55%), Query Frame = 0


Query: 1   MEGGGDGCDLLE----QEGRGGES-----TAASLDGMEAILNYEFRDKRLLEEAFTDASY 60
           ME G    D LE      G GGE+     + ASLDG+EAILNYEFRDKRLLEEAFTDASY
Sbjct: 1   MEEGKKRVDFLEGKDGSRGGGGEAVNGSDSPASLDGIEAILNYEFRDKRLLEEAFTDASY 60

Query: 61  SPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHG 120
           SPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHG
Sbjct: 61  SPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHG 120

Query: 121 FHRYLRHNKADLDQKIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDT 180
           FHRYLRHNKADL QKIEEF ++IEEYPLHSNG+IKAPK+LADIVES IGAVF DSN IDT
Sbjct: 121 FHRYLRHNKADLHQKIEEFGRSIEEYPLHSNGMIKAPKVLADIVESTIGAVFADSNFIDT 180

Query: 181 VWTIFKGLMEPLITLKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGK 240
           VW IFK LMEPLITLKTMK+HPMTELTEMCQKR+LK EF D WEE KEI VLI+KQ+VGK
Sbjct: 181 VWKIFKELMEPLITLKTMKKHPMTELTEMCQKRSLKLEFRDRWEETKEIEVLIEKQVVGK 240

Query: 241 GSYGKKLIAQNRAAKDALDNFHTILGQFNEPCQPM 266
           GSY KKLIAQNRAAK+AL+N  T   QFN+PCQPM
Sbjct: 241 GSYRKKLIAQNRAAKNALENLDTFFPQFNQPCQPM 275

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 425 bits (1093), Expect = 1.95e-148
Identity = 209/243 (86.01%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97

Query: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157

Query: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203
           LIKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217

Query: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277

Query: 264 QPM 266
           QPM
Sbjct: 278 QPM 280

[Close]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL
Match: A0A1Q3BNT8 (Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_13208 PE=4 SV=1)

HSP 1 Score: 310 bits (795), Expect = 1.01e-103
Identity = 158/237 (66.67%), Postives = 192/237 (81.01%), Query Frame = 0


Query: 25  LDGMEAILNYEFRDKRLLEEAFTDASY-SPENCSSFERLEYVGDSVLNFLITREQYFSNP 84
           L+G+E IL Y+F +K LLEEAFT ASY SP+ C+S+ERLE+VGDSVLN L T+EQYF  P
Sbjct: 1   LEGVEEILGYKFGNKSLLEEAFTHASYTSPDQCASYERLEFVGDSVLNLLFTKEQYFLYP 60

Query: 85  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 144
           NL PG LTRLRAANVDTEKLARV+I HG HRYL H K  L+Q+I+EF++ I +YPLHSNG
Sbjct: 61  NLQPGDLTRLRAANVDTEKLARVSIRHGLHRYLCHKKPLLEQQIQEFTQAILDYPLHSNG 120

Query: 145 LIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQ 204
           L+  PK+LADIVES IGAVF DSN SID VW +FKGL+EP+I+++T+KRHP+TEL E+CQ
Sbjct: 121 LVDPPKVLADIVESTIGAVFIDSNLSIDIVWKVFKGLLEPIISVETLKRHPVTELYEVCQ 180

Query: 205 KRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRAAKDALDNFHTILGQ 258
           K+NLK +FVDLW+E     V ID QLVG+ +YG KK IA NRAAKDAL+N   IL +
Sbjct: 181 KKNLKVKFVDLWKESTAFEVFIDDQLVGRDTYGTKKEIAHNRAAKDALNNMWKILNE 237

[Close]

BLAST of Cp4.1LG15g07320 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 249.2 bits (635), Expect = 3.7e-66
Identity = 132/253 (52.17%), Postives = 168/253 (66.40%), Query Frame = 0


Query: 6   DGCDLLEQEGRGGESTAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYV 65
           DG D  +  G         ++ +E ILNY+F+DK LL +AFTDASY  +   S+E LE +
Sbjct: 10  DGKDNTKTIGSADPDQLMEIESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELL 69

Query: 66  GDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQ 125
           GDS+LN  I  +     P  +PG LT+LRA NVDTEKLARVA+ H  + YLRH K  L++
Sbjct: 70  GDSILNMGIIYDFIKLYPKEAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEE 129

Query: 126 KIEEFSKNIEEYPLHSNGLIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLIT 185
           +I EF + +E+YPLHSNGL+K PK+LADIVES IGA+F D NS +TVW + K L+EP+I 
Sbjct: 130 QILEFVEAMEKYPLHSNGLLKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPIIH 189

Query: 186 LKTMKRHPMTELTEMCQKRNLKWEFVDLWEERKEIHVLIDKQLVGKGSYG-KKLIAQNRA 245
           L  MK HPMTEL EMCQKRNLK    D WEE +     I+ + VG G +  KK  A+N A
Sbjct: 190 LDKMKSHPMTELNEMCQKRNLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNFA 249

Query: 246 AKDALDNFHTILG 258
           AK+A+DNF    G
Sbjct: 250 AKNAIDNFAKFFG 262

[Close]

BLAST of Cp4.1LG15g07320 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 171.4 bits (433), Expect = 9.8e-43
Identity = 103/241 (42.74%), Postives = 144/241 (59.75%), Query Frame = 0


Query: 19   ESTAASLD--GMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1551 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1610

Query: 79   EQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1611 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1670

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1671 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1730

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1731 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1790

[Close]

BLAST of Cp4.1LG15g07320 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 171.4 bits (433), Expect = 9.8e-43
Identity = 103/241 (42.74%), Postives = 144/241 (59.75%), Query Frame = 0


Query: 19   ESTAASLD--GMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITR 78
            ES   S+D  G+E  L YEF++K LL EA T AS      S ++RLE+VGD+VL+ LITR
Sbjct: 1552 ESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITR 1611

Query: 79   EQYFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIE- 138
              +F+  +L PG LT LRAA V+ E  ARVA+ H  H YLRH  + L+++I EF K ++ 
Sbjct: 1612 HLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQT 1671

Query: 139  ---EYPLHSNGL--IKAPKILADIVESCIGAVFTDSNSIDT-VWTIFKGLMEPLITLKTM 198
               +   +S GL   KAPK+L DIVES  GA+F DS    T  W +F+ L++P++T +T+
Sbjct: 1672 ESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETL 1731

Query: 199  KRHPMTELTEMCQKRNLKWEF-VDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDA 250
              HP+ EL E CQ++    E+          + V ID   VG     +K +AQ  AA++A
Sbjct: 1732 PMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNA 1791

[Close]

BLAST of Cp4.1LG15g07320 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 171.4 bits (433), Expect = 9.8e-43
Identity = 95/237 (40.08%), Postives = 150/237 (63.29%), Query Frame = 0


Query: 20  STAASLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQY 79
           S   S++ +E ILNY+F +K LL+EA T  S +  +  S+ERLE++GDS +   I+   Y
Sbjct: 55  SEMESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAISNYLY 114

Query: 80  FSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNI---EE 139
            + P+L P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EFS+ +   ++
Sbjct: 115 LTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDD 174

Query: 140 YPLHSNGLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKR--HP 199
             +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IF+GL+EP++TL  +++   P
Sbjct: 175 LSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQP 234

Query: 200 MTELTEMCQKRNLKWEFVDLWEERKEIHVL-IDKQLVGKGSYGKKLIAQNRAAKDAL 250
           ++ L ++C K   + +  +  +    I V+ +D +L+  G    K IA+  AAK+AL
Sbjct: 235 VSMLFKLCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEAL 289

[Close]

BLAST of Cp4.1LG15g07320 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 135.2 bits (339), Expect = 7.7e-32
Identity = 79/189 (41.80%), Postives = 113/189 (59.79%), Query Frame = 0


Query: 28   MEAILNYEFRDKRLLEEAFTDASY-SPENCSSFERLEYVGDSVLNFLITREQYFSNPNLS 87
            ME++LNY F DK LL EA T  SY  PE    ++RLE++GDSVL++LIT+  Y   P LS
Sbjct: 1152 MESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSVLDYLITKHLYDKYPCLS 1211

Query: 88   PGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHS----N 147
            PG LT +R+A+V+ E  A VA+    H+++ +    L + I       E+  L S     
Sbjct: 1212 PGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISRTVSEFEQSSLQSTFGWE 1271

Query: 148  GLIKAPKILADIVESCIGAVFTDSN-SIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMC 207
              I  PK+L D++ES  GA+F DS  + + V+   K L+  +IT +T+K HP+ ELTE+C
Sbjct: 1272 SDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMITPETVKLHPVRELTELC 1331

Query: 208  QKRNLKWEF 211
            Q    KW+F
Sbjct: 1332 Q----KWQF 1336

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g07320 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69KJ0	3.3e-59	50.42	Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0...	[more]
Q9SP32	1.4e-41	42.74	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q9LTQ0	1.4e-41	40.08	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q6ATG6	3.4e-40	40.26	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]
Q8LMR2	2.9e-39	41.03	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]

BLAST of Cp4.1LG15g07320 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023554575.1	7.71e-193	100.00	ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022952611.1	5.00e-189	98.50	ribonuclease 3-like protein 3 [Cucurbita moschata]	[more]
XP_022969213.1	5.00e-189	98.50	ribonuclease 3-like protein 3 [Cucurbita maxima]	[more]
KAG7011897.1	1.01e-188	98.50	Ribonuclease 3-like protein 3, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6572281.1	1.67e-187	98.12	Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cp4.1LG15g07320 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I0C1	2.42e-189	98.50	ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 S...	[more]
A0A6J1GKQ4	2.42e-189	98.50	ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4...	[more]
A0A0A0K1J8	1.47e-151	81.45	RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 P...	[more]
A0A6J1C375	1.95e-148	86.01	ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=...	[more]
A0A1Q3BNT8	1.01e-103	66.67	Ribonuclease_3 domain-containing protein (Fragment) OS=Cephalotus follicularis O...	[more]

BLAST of Cp4.1LG15g07320 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15417.1	3.7e-66	52.17	RNAse II-like 1	[more]
AT1G01040.1	9.8e-43	42.74	dicer-like 1	[more]
AT1G01040.2	9.8e-43	42.74	dicer-like 1	[more]
AT3G20420.1	9.8e-43	40.08	RNAse THREE-like protein 2	[more]
AT3G03300.1	7.7e-32	41.80	dicer-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 40..189 e-value: 3.4E-33 score: 126.3
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 60..166 e-value: 7.6E-22 score: 77.9
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 60..68
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 25..167 score: 33.77779
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 40..185 e-value: 2.76389E-39 score: 131.581
None	No IPR available	GENE3D	3.30.160.20		coord: 193..261 e-value: 4.4E-7 score: 31.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..22
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 22..257
None	No IPR available	PANTHER	PTHR14950:SF54	RNASE II-LIKE 1	coord: 22..257
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 149..252
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 20..192 e-value: 1.5E-48 score: 167.0
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 25..186

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g07320.1	Cp4.1LG15g07320.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding