Cp4.1LG15g06720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g06720
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSubtilisin-like protein protease
LocationCp4.1LG15: 7318749 .. 7325853 (-)
RNA-Seq ExpressionCp4.1LG15g06720
SyntenyCp4.1LG15g06720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGGAAGACATATAATTAAAGCCAAGAAGGGGAGAAAAAACCAGAGACAAGACACAAGAACAAACCATGAAACAACCAACACATCCTTCAGCTACTGTCCTCCTCCTTCTTCTCTCTTCTTTGCTGCTCACTCCCACCGCCGCCATTAAAAGGGTTAGTTCATTTTCTTTGCTTTTCTCTCATTTTCTCTCTCAAATTTTCACCCCATTTGCAGTCCTATGTTGTCTACATGGGAGCTCATTCCCACGGCGGTCGGAAACCCGCCAACATCGTCGCCAATTCTCACCGAGACCTCCTCCGACCATATCTCGAGAGGTACGTTTAAAAAAAAAATCAACTTTATCATTACCCATTAGCCATAATGTTCGAACTCTTGATCGTGTCGAAATCGATTATTTAACCGTTGATTTATTATCTTTTTAGTGGAAATGAATTCGCCGAAGATGTCATCTTTTACTCGTACACTAGACATATCAATGGCTTTGCAGCAATGTTGGAAGACGAAGTAGCTGCTCGATTAGCTCGTAACTATTCTTTCTCTTTTCATTCATCGTTAATATCTCAAATTCGAAGATTTAATACGAAATTTAGAGAAAAAATGTAAAATTTTTAATTATAATATCGATCACGTTTTGAGTTGATTGGTGATTTAACATTTTACGTGTTTAAAACACAGAACACCCGAAAGTGGTGTCGGTTTTTCCGAACCGAGGAAGACGGTTACACACGACACGATCATGGGAGTTCATGGAATTGGAGAACAATCATGGAGTTATAAGTTCAGAATCAATATGGAAGAAGGCAAGGTTTGGAGAAGACACCATTATTGGAAACCTTGATACTGGTTCGTTGTCTCTCTCTAACTTTCTCTCTCTTCTTTAATTAATTAATTATTTATTTATTTATTAAATAATTAATTTTTATTTGAATAATATTTTTTCAAGCCCTTGACTTTTTTTTTTAATTAATAAAAACAAGATCTGATTTTTATTACAGAATCAAAACGACGTAGTTTTGATTAGTTTAATATTTTTTATTTTTGGAGAACTAAAAATATATTTTAATGGATCTGTGTTCAGTCTGTTTGACTCTCTTATCTGCAACCCCAATGGTTGATTTTTTTAATCCATTTTTAATTAATTTATAAAAAAATAAATTATTTTTATATTTTAAATAAATTAAATTAATAAAAATATACTTGAAATTTGAAGAAGCTCGAGAACGAGCATGAAAGATAGAATAAAGAAAAGTAAGAGAATGTAGATTCCAACGAACGGAAAATTGGGCAAAAGTAAAGCGAAAGACTTTAACCAATCAAGACGACGATAGTAACCTCGAGACGATAATTGAGTCTCCAGTGATAGTTGATGATGTTAAAAACTTCAAAACCGACATTGAAAGCAGGTCTATATAATAATTTTAAGAGTGTTAAAAAATATTTTACTAAAAAATTATCGAAAGTATCGGTGTTAAATTTAAATTTTTTAAAAATATAGAGACTAAAATAAAATAAATTTTAAAACGAGTAATAATAACTTATTTATTGTTAGACCGGTGATTGATATAATTGGACTAAGGGGTATTTTGGTCATTTTGGGCATGGAATTTCAACAGGGGTATGGCCGGAATCAAAGAGCTTTAGTGATCACGATGATTTGGGACCAATTCCCCAAAGGTGGAGAGGAATATGCCAAAACCAGAACGATCCTTCTTTCCATTGCAATAGGTACTTTCTCTTTCCCTCTCTTCATTTTTTTTAATTTTTAATTTAAATAGCGGAGTAGAGATTCCGACCTACGTCCCGAGCGACGTTCATATATTAATATCTTAGCTTTTATATTTTATCTTATTTAGAGTTCAAAGTCGAGTATATCAGAATTTTCGATTCTAATTATTTTTACTCCCGTTGTCCATTTAAAAAAAAAAAAAAAAAAAAAGAGCCTAGAACTAAATATAAATCTCGATATGGAGATCAGATATGTACTTAACGCTCTATTTACAGAACTTTCTCTCTACTTATATCACAGTTCTCGGGACTTTAAAAAAAGCAAATTCTAATTATAAATTAATTCCTCAATACAAAACCTACCAACACCCGAACACGAGCGTAACTTAACAATAAAGAAGTCTACCCGTTTTTTTCAAAGGTAAAAGTTTGTCTAGAATTAGTTTAAGTAGGACCTACCAATATCGAGACGAAATTGGGAATATTCAAATCGACTTTAGAAAAGATATAGTCGAGTATATCTTCTTTTCAATTATGCCCATAAGAATAATATTTTTATTTTTTATTCTCTCTTCCCTTTTTTACTTTTTATCTATATATATATATATATATAATATTTATTTTCAATCTAAAATATTAATTTTATATATAAAATTAGTGTGGGGTCAGTTAGCGAACAAACTCGACCAAGCACAGTTCAAACTCGCTTGAGTTGTTCGATTTTCAAAAATTTTAAACCATTACGATTTTAAAACGGGTTCAACGTCCTCGTTGACACACCAATCAGTATCTAGTTTTGGTATCACTTAACAGCTCACTGCTAGTAGTGATATTGTCGGCTTTTGCTCGTTGCATATGGCTGTCGGCCTCAAAGGATTTGAATTATTTCATAGTTTATGCGCATGCACTTTCTTGATATTCTCTGAAATTTCTGTAAATTTGGTGGTAATTTAATCTCAAGGAAACTAATCGGAGCAAGATACTTCAACAAAGGCTACAAATCCGTTGTCGGTCATCTAAATTCTTCGTTCAATTCGCCGAGGGATAATGAAGGGCATGGTTCTCATACCTTATCGACGGCGGGCGGTAACTTCGTCGCCGGAGCTAGTGTTTTCGGCTTGGGAAAGGGCATTGCCAAGGGCGGATCGCCTAGGGCTCGGGTGGCCGCTTACAAGGTCTGCTGGCCTCCAGCGGCTGGGAATGAGTGCTTCGATGCCGATATATTGGCGGCGTTTGATGTGGCGATCCACGATGGCGTCGATGTGTTGTCGGTGTCGCTTGGAGGAGATCCGAATCCGTTGTTTAACGATAGTGTTGCGATTGGATCATTCCACGCCGTGAAGCACGGGATTGTGGTGATTTGCTCTGCCGGAAATTCGGGACCGACAGCCGGTTCCGTGACGAATATTGCGCCCTGGCAGATCACCGTTGGAGCCAGCACCATGGACAGGAAGTTCCCTAGTCGCGTCGTTCTTGGGAACAAGAAGCAGATTGAGGTTAGTGCAACTTGTTCTTGAAATTATGTAGAGAGGAAATGAAGTTTGATGCAACATTTATGCTATGAAAGGAATTTCAAACCACAACAGATTTGAAACATAGGTTAAATTACAAAATCTTTTGTGCATAAACTTGAATTTTATACCTAACCAATCGTTAAACTTTCAATTTCATGAACTTCAAAAGTTGTCAAACAAGCCAATGATCGATTATAAGAATTATATGTTAGGAATCACGACTCTCTGCAATGATATGATATTGTCTACTTTGAGCATAACTTATTGTGTTTGGCTTTGGTTTCCTCAAAAAGGACTCATACCACGGAGATGTATTCCTTCCTTATAAACTCATGATCATCCTCGTTGACTACTTTGAGCATAACTTCTTGTGGCTTTGGCTTTGGTTTCCTCAAAAGGACTCATACCACGGAGATGTATTCCTTCCTTATAAACTCATGATCATCCCCTTGATTAGTCAACTTGGGACTCCCTCCCAACAATCCTCCCCTTGATCGAAGTACACCATAGAGTCTCCCCTGAGATCTATGGAGCCCTCGAACAGCCTCCCCTTAATTGAGGCTCGACTCCTTTCTCTGGAGCCCTCGAACAAAGTACACCCTTTGTTCAACACTTGAGTCACTTTTTACTACACCTTTGAGGGTCCCAACTTCTTTGTTCGACATTTAAGGATTTTGTTGACAGGACTAAGTTAAGGGCATGACTCTGATACCATGTTAGGAATCACGACTCGCCATAATGGTATGATGTTGTCCACTTTGAGCATAAGCTCTCAAAAGACCTCATATCAATGGAGATAGTATTCCTTACTTATAAATCCATGATCATTCCCTCCCAACAATCCTCAACCGTATAAGGACTTCCATAAAATCGAAAGTTTAGGATCTACTTGATACAAACTTGAAAGTTTATCAATGGTGCAATTTGTCTAACGAAGAACACAATGTTTTGTAGGGCGAAAGCCTAACCCCTGAAGGTTTGCCAAGCAGGAAGTTCTATCCACTCATGAGTGCTGCAGATGTTAGATTGGCCAATGCATCAGCTCATGAAGCGTAAGAACATAGAACAATAATTTTCTGCTCTGTCTTAGCTATTGAAATTACCTTAGAATTGTTGTGTTTAGGGTCTTTTTAGATTGAATTTCTAAGTGTTCATAGTAATTGGCTCTGATTTATTGAACATTTCAGTCAATTGTGTAAAGCTGGTACGCTTGATCCTAAGAAGGCGAAGGGAAAGATCCTTGTCTGCCTTCGTGGTGATAATGCAAGAGTGGACAAGGGCGAGCAAGCAATGTTGGCTGGTGCTGTGGGTATGATTCTCGCCAACAATGAGCTCAGTGGGAATGAAATCCTTGCTGACCCACATGTTCTTCCAGCCTCGCACATCAACTTCACTGATGGCGTTGCAGTTTTTGCATACATCAATTCAACCAAGTAAGTTGCTTCTATAACAGTAACAAGCATGTTTAGATTAACTTTCTAAATGCTTAAAAACATTTTCAAACATTTGGAATGTCAATCCAAACCGGTCCTAAATTAAGCACAACGGCAAACAACATTTGAGTTGTTTGGACCGTATGACTGTCCTGTATCACAATCGCTCTTTTCTTGACAACGGACTTTGCGATTGTGCAACACTCACTCCCAACACCAAGTGAGTCAAGCCAATTTGTATTCGTGTCGCCTACGGCTGGGTAATGTATGCTTGAGCCTTTGGCCCCATTTGAGAACAAGGTTTTAGAAAACGGGGATAGAGAGATTTTTGAAAGCGAGTTTGAAAGTAAGAAAGAGAGAAGATTTTAAAAGCAACGTTTTATAGCTATAAGCAAAAGGCAACACAACGGCTTAATAACATATAACTTTCTGGATAGGAAGAATTTGACAACTAGTCGCATCCCCTTATAATACATTTTGAGGCGATTCAGATCTGGCAGGCCTAGACGTGGTTCCGCCGAACGGTCATACAAAATCAGGGGGAGGAGTTGACAACTAGTCACACCCTCCCTATAGTACAAATGGCCTAGACATGACTTCGCCGAGTAGTCGTACAAATAGGAAATACAATAGTAAGGCAATACAACATGAAATTAAGTAAAGGGTTCGGCATGACATGGACATGTGGCTACAAGCAACACACCCTAAACGAACCTGACCGTGACACCCTGCACATGGTCTATTATGTACATCACCATAAAGTAATTGAAACCTTTAAATTGTGAACAGGTTCCCTCAGGCTTACATCACAGGAGCTACAACTCAATTAGGTATAAGGCCAGCTCCATTTATGGCAGCATTTTCATCAGTTGGACCAAGCACTATTACTCCAGAGATCTTAAAGGTTGTCTTTTAGGCTCCATTTGATACTCATTTAGTTCATAAAATACGTGTCTGGTTTCTCACACTTCTTTATAATGGTATTCATCCAAATAGAAACTTATTGTTTATAAACTTAATTTTTTAAGTCAAATGGATATCAAACGGGGCTATAATGATTGTTGGGTGATTTTTGTGTGGGCAGCCTGATGTTACAGCACCTGGGCTGAGTGTTATAGCTGCTTATACTGGAGCAGAAGGACCTACCAATCAAGAATATGATAAGCGTCGGGTTGCTTTTAACTCGGTATCAGGAACTTCCATGTCATGCCCTCATGTTTCTGGTATAGCGGGTCTCCTTAGAACTCTTTACCCTCACTGGAGTCCTGCCGCTATTAAATCTGCAATCATGACCACGGGTAAAACAATTCAACGCCCTCGGTATTTTCTTTGCCACGAATCTTTGAAAACAAATAATCGGTCCTTATGTTTCGACTATATTTTCCATGTTCTTATATTCGTTACTCTGAGTTTAGTTTCCATTTGGTCCTAACGTTTCAAAAAGTTACATTTTTACCATAATTTTTTAGTTTAGTTTCCGTTTAGTCATAGATTTCAAAATATTGCATCTTTTTTATCCTCGAGTTTTGAGTTTAGTTTCCATTTGGGTCCTAAGTTTCTAGATTTGACACTTTTACCTCAAGTTTGTACTAATTCTCATTCTTGGTCATTAGTGTTAGCTTTGATTAAAAGTGAGCGTTGGTGAAAACTCACGAACACTGCCCTAAGTTTCTAGATTTGACACTTTTACTTCGAGTTTGTACTAATTCTCGTTTTTTTGGTCATTGGTGTTAGCTTTGGTTAAAAGTGAGCATTGGTGAAAGCTCACGAGCTGAGGGTAAAAATGTAATATTTTGAAAATTTAAGGACCAAATGGAAACTAAACGGACAAGTTAGAAGTAAAAGTGTGACATTTTAGCGTCTTTCTTATTAGCTTCTATTTTTCACAGCAAGCACCTTAGACAACAACTTCAAGCCGCTCCTCAATGCTTCCTACTCTGTGGCCACGCCATTCAACTATGGAGCAGGACACATCCATCCAAATAGAGCTACAGATCCTGGCTTGGTCTATGACATAACGGTTAATGAATATCTGTCATTTCTATGTGCCTTAGGATACAACAAAGCTCAACTATCACAGTTCTCCAATGGCCCCTTCAATTGCTCGGAGCCTATTAGTCTTACAAATCTCAACTATCCTTCAATCACTGTCCCTAACCTCTCGAGATCGATCACAATTACTCGAAGACTCAAGAACGTCGGTTCTCCCGGAACGTACAAAGCCGAGATAAGAAAACCAGCTGGGATATCGGTTTCGGTTAAGCCAAAGAAGTTGAGCTTCACAAGATTGGGTGAAGAGCTGAGCTTCAAAGTTCTCATGAAAGTCAAGGAAGGGAATGTAGGAAGGAAGAAGAATTATGTATATGGTGATTTGATATGGTCTGATGGTAAGCACCATGTAAGAAGCCCTATTGTGGTAATGGCAGTCTAG

mRNA sequence

GGAGGAAGACATATAATTAAAGCCAAGAAGGGGAGAAAAAACCAGAGACAAGACACAAGAACAAACCATGAAACAACCAACACATCCTTCAGCTACTGTCCTCCTCCTTCTTCTCTCTTCTTTGCTGCTCACTCCCACCGCCGCCATTAAAAGGTCCTATGTTGTCTACATGGGAGCTCATTCCCACGGCGGTCGGAAACCCGCCAACATCGTCGCCAATTCTCACCGAGACCTCCTCCGACCATATCTCGAGAGTGGAAATGAATTCGCCGAAGATGTCATCTTTTACTCGTACACTAGACATATCAATGGCTTTGCAGCAATGTTGGAAGACGAAGTAGCTGCTCGATTAGCTCAACACCCGAAAGTGGTGTCGGTTTTTCCGAACCGAGGAAGACGGTTACACACGACACGATCATGGGAGTTCATGGAATTGGAGAACAATCATGGAGTTATAAGTTCAGAATCAATATGGAAGAAGGCAAGGTTTGGAGAAGACACCATTATTGGAAACCTTGATACTGGGGTATGGCCGGAATCAAAGAGCTTTAGTGATCACGATGATTTGGGACCAATTCCCCAAAGGTGGAGAGGAATATGCCAAAACCAGAACGATCCTTCTTTCCATTGCAATAGATACTTCAACAAAGGCTACAAATCCGTTGTCGGTCATCTAAATTCTTCGTTCAATTCGCCGAGGGATAATGAAGGGCATGGTTCTCATACCTTATCGACGGCGGGCGGTAACTTCGTCGCCGGAGCTAGTGTTTTCGGCTTGGGAAAGGGCATTGCCAAGGGCGGATCGCCTAGGGCTCGGGTGGCCGCTTACAAGGTCTGCTGGCCTCCAGCGGCTGGGAATGAGTGCTTCGATGCCGATATATTGGCGGCGTTTGATGTGGCGATCCACGATGGCGTCGATGTGTTGTCGGTGTCGCTTGGAGGAGATCCGAATCCGTTGTTTAACGATAGTGTTGCGATTGGATCATTCCACGCCGTGAAGCACGGGATTGTGGTGATTTGCTCTGCCGGAAATTCGGGACCGACAGCCGGTTCCGTGACGAATATTGCGCCCTGGCAGATCACCGTTGGAGCCAGCACCATGGACAGGAAGTTCCCTAGTCGCGTCGTTCTTGGGAACAAGAAGCAGATTGAGTTCTATCCACTCATGAGTGCTGCAGATGTTAGATTGGCCAATGCATCAGCTCATGAAGCTCAATTGTGTAAAGCTGGTACGCTTGATCCTAAGAAGGCGAAGGGAAAGATCCTTGTCTGCCTTCGTGGTGATAATGCAAGAGTGGACAAGGGCGAGCAAGCAATGTTGGCTGGTGCTGTGGGTATGATTCTCGCCAACAATGAGCTCAGTGGGAATGAAATCCTTGCTGACCCACATGTTCTTCCAGCCTCGCACATCAACTTCACTGATGGCGTTGCAGTTTTTGCATACATCAATTCAACCAAGTTCCCTCAGGCTTACATCACAGGAGCTACAACTCAATTAGGTATAAGGCCAGCTCCATTTATGGCAGCATTTTCATCAGTTGGACCAAGCACTATTACTCCAGAGATCTTAAAGCCTGATGTTACAGCACCTGGGCTGAGTGTTATAGCTGCTTATACTGGAGCAGAAGGACCTACCAATCAAGAATATGATAAGCGTCGGGTTGCTTTTAACTCGGTATCAGGAACTTCCATGTCATGCCCTCATGTTTCTGGTATAGCGGGTCTCCTTAGAACTCTTTACCCTCACTGGAGTCCTGCCGCTATTAAATCTGCAATCATGACCACGGGACACATCCATCCAAATAGAGCTACAGATCCTGGCTTGGTCTATGACATAACGGTTAATGAATATCTGTCATTTCTATGTGCCTTAGGATACAACAAAGCTCAACTATCACAGTTCTCCAATGGCCCCTTCAATTGCTCGGAGCCTATTAGTCTTACAAATCTCAACTATCCTTCAATCACTGTCCCTAACCTCTCGAGATCGATCACAATTACTCGAAGACTCAAGAACGTCGGTTCTCCCGGAACGTACAAAGCCGAGATAAGAAAACCAGCTGGGATATCGGTTTCGGTTAAGCCAAAGAAGTTGAGCTTCACAAGATTGGGTGAAGAGCTGAGCTTCAAAGTTCTCATGAAAGTCAAGGAAGGGAATGTAGGAAGGAAGAAGAATTATGTATATGGTGATTTGATATGGTCTGATGGTAAGCACCATGTAAGAAGCCCTATTGTGGTAATGGCAGTCTAG

Coding sequence (CDS)

ATGAAACAACCAACACATCCTTCAGCTACTGTCCTCCTCCTTCTTCTCTCTTCTTTGCTGCTCACTCCCACCGCCGCCATTAAAAGGTCCTATGTTGTCTACATGGGAGCTCATTCCCACGGCGGTCGGAAACCCGCCAACATCGTCGCCAATTCTCACCGAGACCTCCTCCGACCATATCTCGAGAGTGGAAATGAATTCGCCGAAGATGTCATCTTTTACTCGTACACTAGACATATCAATGGCTTTGCAGCAATGTTGGAAGACGAAGTAGCTGCTCGATTAGCTCAACACCCGAAAGTGGTGTCGGTTTTTCCGAACCGAGGAAGACGGTTACACACGACACGATCATGGGAGTTCATGGAATTGGAGAACAATCATGGAGTTATAAGTTCAGAATCAATATGGAAGAAGGCAAGGTTTGGAGAAGACACCATTATTGGAAACCTTGATACTGGGGTATGGCCGGAATCAAAGAGCTTTAGTGATCACGATGATTTGGGACCAATTCCCCAAAGGTGGAGAGGAATATGCCAAAACCAGAACGATCCTTCTTTCCATTGCAATAGATACTTCAACAAAGGCTACAAATCCGTTGTCGGTCATCTAAATTCTTCGTTCAATTCGCCGAGGGATAATGAAGGGCATGGTTCTCATACCTTATCGACGGCGGGCGGTAACTTCGTCGCCGGAGCTAGTGTTTTCGGCTTGGGAAAGGGCATTGCCAAGGGCGGATCGCCTAGGGCTCGGGTGGCCGCTTACAAGGTCTGCTGGCCTCCAGCGGCTGGGAATGAGTGCTTCGATGCCGATATATTGGCGGCGTTTGATGTGGCGATCCACGATGGCGTCGATGTGTTGTCGGTGTCGCTTGGAGGAGATCCGAATCCGTTGTTTAACGATAGTGTTGCGATTGGATCATTCCACGCCGTGAAGCACGGGATTGTGGTGATTTGCTCTGCCGGAAATTCGGGACCGACAGCCGGTTCCGTGACGAATATTGCGCCCTGGCAGATCACCGTTGGAGCCAGCACCATGGACAGGAAGTTCCCTAGTCGCGTCGTTCTTGGGAACAAGAAGCAGATTGAGTTCTATCCACTCATGAGTGCTGCAGATGTTAGATTGGCCAATGCATCAGCTCATGAAGCTCAATTGTGTAAAGCTGGTACGCTTGATCCTAAGAAGGCGAAGGGAAAGATCCTTGTCTGCCTTCGTGGTGATAATGCAAGAGTGGACAAGGGCGAGCAAGCAATGTTGGCTGGTGCTGTGGGTATGATTCTCGCCAACAATGAGCTCAGTGGGAATGAAATCCTTGCTGACCCACATGTTCTTCCAGCCTCGCACATCAACTTCACTGATGGCGTTGCAGTTTTTGCATACATCAATTCAACCAAGTTCCCTCAGGCTTACATCACAGGAGCTACAACTCAATTAGGTATAAGGCCAGCTCCATTTATGGCAGCATTTTCATCAGTTGGACCAAGCACTATTACTCCAGAGATCTTAAAGCCTGATGTTACAGCACCTGGGCTGAGTGTTATAGCTGCTTATACTGGAGCAGAAGGACCTACCAATCAAGAATATGATAAGCGTCGGGTTGCTTTTAACTCGGTATCAGGAACTTCCATGTCATGCCCTCATGTTTCTGGTATAGCGGGTCTCCTTAGAACTCTTTACCCTCACTGGAGTCCTGCCGCTATTAAATCTGCAATCATGACCACGGGACACATCCATCCAAATAGAGCTACAGATCCTGGCTTGGTCTATGACATAACGGTTAATGAATATCTGTCATTTCTATGTGCCTTAGGATACAACAAAGCTCAACTATCACAGTTCTCCAATGGCCCCTTCAATTGCTCGGAGCCTATTAGTCTTACAAATCTCAACTATCCTTCAATCACTGTCCCTAACCTCTCGAGATCGATCACAATTACTCGAAGACTCAAGAACGTCGGTTCTCCCGGAACGTACAAAGCCGAGATAAGAAAACCAGCTGGGATATCGGTTTCGGTTAAGCCAAAGAAGTTGAGCTTCACAAGATTGGGTGAAGAGCTGAGCTTCAAAGTTCTCATGAAAGTCAAGGAAGGGAATGTAGGAAGGAAGAAGAATTATGTATATGGTGATTTGATATGGTCTGATGGTAAGCACCATGTAAGAAGCCCTATTGTGGTAATGGCAGTCTAG

Protein sequence

MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCNRYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV
Homology
BLAST of Cp4.1LG15g06720 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 896.0 bits (2314), Expect = 2.9e-259
Identity = 458/772 (59.33%), Postives = 562/772 (72.80%), Query Frame = 0

Query: 5   THPSATVLLLLLSSLLLTPTAAIK--RSYVVYMGAHSHGG---RKPANIVANSHRDLLRP 64
           TH  + +LLLLL  +      A K   SYVVY GAHSH G       + V  +H D L  
Sbjct: 4   THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGS 63

Query: 65  YLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWE 124
           +  S  E A D IFYSYT+HINGFAA L+ ++A  +++HP+VVSVFPN+  +LHTTRSW+
Sbjct: 64  FTGS-RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWD 123

Query: 125 FMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQ 184
           F+ LE+N   + S SIW+KARFGEDTII NLDTGVWPESKSF D + LGPIP RW+GICQ
Sbjct: 124 FLGLEHN-SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRD-EGLGPIPSRWKGICQ 183

Query: 185 NQNDPSFHCN------RYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGAS 244
           NQ D +FHCN      RYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+FV G S
Sbjct: 184 NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVS 243

Query: 245 VFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGD 304
           +FG G G AKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+SVSLGG+
Sbjct: 244 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 303

Query: 305 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRV 364
           P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +V+N+APWQITVGASTMDR+F S +
Sbjct: 304 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 363

Query: 365 VLGNKK-------------QIEFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKIL 424
           VLGN K               +FYP+M++ + +  NASA +AQLCK G+LDP K KGKIL
Sbjct: 364 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 423

Query: 425 VCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYI 484
           VCLRG N RV+KG    L G +GM+L N  ++GN++LADPHVLPA+ +   D  AV  YI
Sbjct: 424 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 483

Query: 485 NSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAE 544
           + TK P A+IT + T LG++PAP MA+FSS GPS + P+ILKPD+TAPG+SVIAAYTGA 
Sbjct: 484 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 543

Query: 545 GPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT------- 604
            PTN+++D RR+ FN++SGTSMSCPH+SGIAGLL+T YP WSPAAI+SAIMTT       
Sbjct: 544 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 603

Query: 605 -------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSN 664
                              GH+ PN A +PGLVYD+ + +YL+FLC+LGYN +Q+S FS 
Sbjct: 604 PGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 663

Query: 665 GPFNCSEP-ISLTNLNYPSITVPNLSRS-ITITRRLKNVGSPGTYKAEIRKPAGISVSVK 724
             F CS P ISL NLNYPSITVPNL+ S +T++R +KNVG P  Y  ++  P G+ V+VK
Sbjct: 664 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 723

BLAST of Cp4.1LG15g06720 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 2.4e-237
Identity = 424/761 (55.72%), Postives = 540/761 (70.96%), Query Frame = 0

Query: 13  LLLLSSLLLTPTAAIKRSYVVYMGAHSHGGR-KPANI--VANSHRDLLRPYLESGNEFAE 72
           LLLL +L  +P  A+K+SY+VY+G+H+H  +   A++  VA+SHR  L  ++ S +E A+
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGS-HENAK 83

Query: 73  DVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGV 132
           + IFYSY RHINGFAA+L++  AA +A+HP VVSVFPN+GR+LHTT SW FM L  N GV
Sbjct: 84  EAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN-GV 143

Query: 133 ISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCN 192
           +   S+W KA +GEDTII NLDTGVWPESKSFSD +  G +P RW+G C         CN
Sbjct: 144 VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSD-EGYGAVPARWKGRCHK----DVPCN 203

Query: 193 ------RYFNKGYKSVVG-HLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIA 252
                 RYFNKGY +  G   N+S+ + RD++GHGSHTLSTA GNFV GA+VFG+G G A
Sbjct: 204 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 263

Query: 253 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSV 312
            GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D +
Sbjct: 264 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 323

Query: 313 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQI- 372
           AIGSFHAVK+G+ V+CSAGNSGP +G+V+N+APW ITVGAS+MDR+F + V L N +   
Sbjct: 324 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 383

Query: 373 -----------EFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARV 432
                      + Y L+SAAD  +AN +  +A LCK G+LDPKK KGKILVCLRGDNARV
Sbjct: 384 GTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 443

Query: 433 DKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYI 492
           DKG QA  AGA GM+L N++ SGNEI++D HVLPAS I++ DG  +F+Y++STK P+ YI
Sbjct: 444 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 503

Query: 493 TGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKR 552
              T  L  +PAPFMA+FSS GP+TITP ILKPD+TAPG+++IAA+T A GPT+ + D R
Sbjct: 504 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 563

Query: 553 RVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT----------------- 612
           R  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAI+SAIMTT                 
Sbjct: 564 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 623

Query: 613 ---------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGP-FNCSEPI 672
                    GH+ PN+A  PGLVYD+T  +YL FLCA+GYN   +  F+  P + C +  
Sbjct: 624 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 683

Query: 673 SLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGE 725
           +L + NYPSITVPNL+ SIT+TR+LKNVG P TY A  R+P G+ VSV+PK+L+F + GE
Sbjct: 684 NLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGE 743

BLAST of Cp4.1LG15g06720 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 624.4 bits (1609), Expect = 1.6e-177
Identity = 361/779 (46.34%), Postives = 476/779 (61.10%), Query Frame = 0

Query: 16  LSSLLLTPTAAIKRSYVVYMGAHSHGGRKPA---NIVANSHRDLLRPYLESGNEFAEDVI 75
           L + LL      K+ Y+VYMGAHSHG    +    +  +SH DLL     S  E A++ I
Sbjct: 17  LFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGS-REKAKEAI 76

Query: 76  FYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVISS 135
            YSY RHINGFAA+LE+E AA +A++P VVSVF ++  +LHTTRSWEF+ L         
Sbjct: 77  IYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR----GQ 136

Query: 136 ESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWR-GICQNQNDPSF---HC 195
            S W+K RFGE+TIIGN+DTGVWPES+SFSD    G +P +WR G+CQ    P      C
Sbjct: 137 NSAWQKGRFGENTIIGNIDTGVWPESQSFSD-KGYGTVPSKWRGGLCQINKLPGSMKNTC 196

Query: 196 N------RYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIA 255
           N      RY+NK +++  G L+   ++ RD  GHG+HTLSTAGGNFV GA VF +G G A
Sbjct: 197 NRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTA 256

Query: 256 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGD----PNPLF 315
           KGGSPRARVAAYKVCW       C+ AD+LAA D AI DGVDV++VS G         +F
Sbjct: 257 KGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIF 316

Query: 316 NDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNK 375
            D ++IG+FHA+   I+++ SAGN GPT G+V N+APW  T+ AST+DR F S + + N+
Sbjct: 317 TDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQ 376

Query: 376 -----------KQIEFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLR-GD 435
                         + + L+ + D +LANA+  +AQLC+ GTLD  K  GKI++C R G 
Sbjct: 377 LIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGK 436

Query: 436 NARVDKGEQAMLAGAVGMILANNELSGNEILADPHVL------PASHINFTDGVAVFAYI 495
              V +G +A+ AGA GMIL N   +G  + A+PHV       P    +   GV   A I
Sbjct: 437 IKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTA-I 496

Query: 496 NSTKFP-----QAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAA 555
                P        ++ A T  G +PAP MA+FSS GP+ I P ILKPDVTAPG++++AA
Sbjct: 497 GDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAA 556

Query: 556 YTGAEGPTNQEYDKRR-VAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT- 615
           Y+     ++   D RR   FN + GTSMSCPH SGIAGLL+T +P WSPAAIKSAIMTT 
Sbjct: 557 YSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTA 616

Query: 616 --------------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKA 675
                                     GH+ P+ A +PGLVYD+++ +YL+FLCA GY++ 
Sbjct: 617 TTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQ 676

Query: 676 QLSQFS-NGPFNCSEPISLTNLNYPSITVPNLS-RSITITRRLKNVGSPGTYKAEIRKPA 725
            +S  + N  F CS   S+ +LNYPSIT+PNL  + +TI R + NVG P TY    R P 
Sbjct: 677 LISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPN 736

BLAST of Cp4.1LG15g06720 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.5e-154
Identity = 323/767 (42.11%), Postives = 455/767 (59.32%), Query Frame = 0

Query: 8   SATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPYLESGNEF 67
           +A  LLL L    ++ +++ + +Y+V+M       + P++   + H +     L S ++ 
Sbjct: 9   TAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSSLRSISDS 68

Query: 68  AEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNH 127
           AE  + Y+Y   I+GF+  L  E A  L   P V+SV P     LHTTR+  F+ L+ + 
Sbjct: 69  AE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 128

Query: 128 GVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQ-NQNDPSF 187
               +  ++ +A    D ++G LDTGVWPESKS+SD +  GPIP  W+G C+   N  + 
Sbjct: 129 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSD-EGFGPIPSSWKGGCEAGTNFTAS 188

Query: 188 HCN------RYFNKGYKSVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASVFGLG 247
            CN      R+F +GY+S +G ++ S    SPRD++GHG+HT STA G+ V GAS+ G  
Sbjct: 189 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 248

Query: 248 KGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLF 307
            G A+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG  +  +
Sbjct: 249 SGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 308

Query: 308 NDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNK 367
            D VAIG+F A++ GI+V CSAGN+GP++ S++N+APW  TVGA T+DR FP+  +LGN 
Sbjct: 309 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 368

Query: 368 KQI--------EFYPLMSAADVRLANAS-AHEAQLCKAGTLDPKKAKGKILVCLRGDNAR 427
           K          E  P      +   NAS A    LC  GTL P+K KGKI++C RG NAR
Sbjct: 369 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 428

Query: 428 VDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAY 487
           V KG+    AG VGMILAN   +G E++AD H+LPA+ +    G  +  Y+ +   P A 
Sbjct: 429 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 488

Query: 488 ITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDK 547
           I+   T +G++P+P +AAFSS GP++ITP ILKPD+ APG++++AA+TGA GPT    D 
Sbjct: 489 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 548

Query: 548 RRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT---------------- 607
           RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAI+SA+MTT                
Sbjct: 549 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 608

Query: 608 -----------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNC--S 667
                      GH+ P  AT+PGL+YD+T  +YL FLCAL Y   Q+   S   + C  S
Sbjct: 609 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 668

Query: 668 EPISLTNLNYPSITVPNLS--RSITITRRLKNVGSPGTYKAEI-RKPAGISVSVKPKKLS 725
           +  S+ +LNYPS  V N+    +   TR + +VG  GTY  ++  +  G+ +SV+P  L+
Sbjct: 669 KSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 728

BLAST of Cp4.1LG15g06720 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 1.4e-152
Identity = 311/719 (43.25%), Postives = 425/719 (59.11%), Query Frame = 0

Query: 72  IFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVIS 131
           I ++Y    +GF+A L  + A++L  HP V+SV P + R LHTTRS EF+ L +      
Sbjct: 62  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST----D 121

Query: 132 SESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGIC-QNQNDPSFHCN- 191
              + +++ FG D +IG +DTGVWPE  SF D   LGP+P +W+G C  +Q+ P   CN 
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDR-GLGPVPIKWKGQCIASQDFPESACNR 181

Query: 192 -----RYFNKGYKSVVGHLN--SSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIA 251
                R+F  GY++  G +N  + F SPRD++GHG+HT S + G +V  AS  G   G+A
Sbjct: 182 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 241

Query: 252 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSV 311
            G +P+AR+AAYKVCW     + C+D+DILAAFD A+ DGVDV+S+S+GG   P + D++
Sbjct: 242 AGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAI 301

Query: 312 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQI- 371
           AIG+F A+  GI V  SAGN GP A +VTN+APW  TVGA T+DR FP+ V LGN K I 
Sbjct: 302 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 361

Query: 372 -------------EFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNA 431
                          YPL+      L     + + LC  G+LDP   KGKI++C RG N+
Sbjct: 362 GVSVYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 421

Query: 432 RVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN------S 491
           R  KGE     G +GMI+AN    G  ++AD HVLPA+ +  + G  +  YI+      S
Sbjct: 422 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 481

Query: 492 TKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGP 551
           +K P A I    T+LGIRPAP +A+FS+ GP+  TPEILKPDV APGL+++AA+    GP
Sbjct: 482 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 541

Query: 552 TNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT--------- 611
           +    D RR  FN +SGTSM+CPHVSG+A LL+  +P WSPAAI+SA++TT         
Sbjct: 542 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 601

Query: 612 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 671
                             GH+HP +A DPGLVYDIT  +Y++FLC   Y +  +   +  
Sbjct: 602 PMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 661

Query: 672 PFNCS---EPISLTNLNYPSITV-----PNLSRSITITRRLKNVG-SPGTYKAEIRKPAG 725
             +C        + NLNYPS +V          S    R + NVG S   Y+ +IR P G
Sbjct: 662 QADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRG 721

BLAST of Cp4.1LG15g06720 vs. NCBI nr
Match: XP_023554401.1 (subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1433 bits (3710), Expect = 0.0
Identity = 727/772 (94.17%), Postives = 727/772 (94.17%), Query Frame = 0

Query: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60
           MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY
Sbjct: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60

Query: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120
           LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF
Sbjct: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120

Query: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180
           MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN
Sbjct: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180

Query: 181 QNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           QNDPSFHCNR      YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 181 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240

Query: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300
           FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP
Sbjct: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360
           NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV
Sbjct: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360

Query: 361 LGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420
           LGNKKQIE             FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV
Sbjct: 361 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420

Query: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480
           CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN
Sbjct: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480

Query: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540
           STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG
Sbjct: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540

Query: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT-------- 600
           PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT        
Sbjct: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 600

Query: 601 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660
                             GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG
Sbjct: 601 KPLLNASYSVATPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660

Query: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720
           PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK
Sbjct: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720

Query: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV
Sbjct: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of Cp4.1LG15g06720 vs. NCBI nr
Match: XP_022952697.1 (subtilisin-like protease SBT5.3 [Cucurbita moschata])

HSP 1 Score: 1421 bits (3678), Expect = 0.0
Identity = 719/772 (93.13%), Postives = 727/772 (94.17%), Query Frame = 0

Query: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60
           MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVA+SHRDLLRPY
Sbjct: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVADSHRDLLRPY 60

Query: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120
           LESGNEFAEDVIFYSYTRHINGFAAMLED+VAARLAQHP+VVSVFPNRGRRLHTTRSWEF
Sbjct: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDQVAARLAQHPEVVSVFPNRGRRLHTTRSWEF 120

Query: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180
           MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSD+DDLGPIPQRWRGICQN
Sbjct: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 180

Query: 181 QNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           QNDPSFHCNR      YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 181 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240

Query: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300
           FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP
Sbjct: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360
           NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV
Sbjct: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360

Query: 361 LGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420
           LGNKKQIE             FYPLMSAA+VRLANASAHEAQLCKAGTLDPKKAKGKILV
Sbjct: 361 LGNKKQIEGESLTPEGLPSRKFYPLMSAANVRLANASAHEAQLCKAGTLDPKKAKGKILV 420

Query: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480
           CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAY+N
Sbjct: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYVN 480

Query: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540
           STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG
Sbjct: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540

Query: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT-------- 600
           PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAI+TT        
Sbjct: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIVTTASTLDNNF 600

Query: 601 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660
                             GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG
Sbjct: 601 KPLLNASYSVATPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660

Query: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720
           PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKA+IRKPAGISVSVKPKK
Sbjct: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAKIRKPAGISVSVKPKK 720

Query: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV
Sbjct: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of Cp4.1LG15g06720 vs. NCBI nr
Match: XP_022968976.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1404 bits (3633), Expect = 0.0
Identity = 709/772 (91.84%), Postives = 722/772 (93.52%), Query Frame = 0

Query: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60
           MKQPT+PS T LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPA+IVA+SHR+LLRPY
Sbjct: 23  MKQPTYPSTTFLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADSHRELLRPY 82

Query: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120
           LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLA+HP+VVSVFPNRGRRLHTTRSWEF
Sbjct: 83  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRLHTTRSWEF 142

Query: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180
           MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSD+DDLGPIPQRWRGICQN
Sbjct: 143 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 202

Query: 181 QNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           QNDPSFHCNR      YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 203 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 262

Query: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300
           FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP
Sbjct: 263 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 322

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360
           NPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV
Sbjct: 323 NPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 382

Query: 361 LGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420
           LGNKKQIE             FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV
Sbjct: 383 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 442

Query: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480
           CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN
Sbjct: 443 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 502

Query: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540
           STKFPQAYITGATTQLG RPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYT AEG
Sbjct: 503 STKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTEAEG 562

Query: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT-------- 600
           PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT        
Sbjct: 563 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 622

Query: 601 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660
                             GHIHPN+ATDPGLVYDIT+ EYLSFLCALGYNKAQLSQFSNG
Sbjct: 623 KPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKAQLSQFSNG 682

Query: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720
           PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIR+PAGISVSVKPKK
Sbjct: 683 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGISVSVKPKK 742

Query: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           LSFTRLGEELSFKVLMKVKEGNVGR+KNYVYGDLIWSDGKHHVRSPIVVMA+
Sbjct: 743 LSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of Cp4.1LG15g06720 vs. NCBI nr
Match: KAG6572205.1 (Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1374 bits (3556), Expect = 0.0
Identity = 693/743 (93.27%), Postives = 698/743 (93.94%), Query Frame = 0

Query: 30  SYVVYMGAHSHGGRKPANIVANSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLED 89
           SYVVYMGAHSHGGRKPANIVA+SHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLED
Sbjct: 37  SYVVYMGAHSHGGRKPANIVADSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLED 96

Query: 90  EVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGN 149
           EVAARLAQHP+VVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGN
Sbjct: 97  EVAARLAQHPEVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGN 156

Query: 150 LDTGVWPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCNR------YFNKGYKSVVGHL 209
           LDTGVWPESKSFSD+DDLGPIPQRWRGICQNQNDPSFHCNR      YFNKGYKSVVGHL
Sbjct: 157 LDTGVWPESKSFSDYDDLGPIPQRWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHL 216

Query: 210 NSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAG 269
           NSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAG
Sbjct: 217 NSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAG 276

Query: 270 NECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNS 329
           NECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNS
Sbjct: 277 NECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNS 336

Query: 330 GPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIE-------------FYPLMSAA 389
           GPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIE             FYPLMSAA
Sbjct: 337 GPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAA 396

Query: 390 DVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNE 449
           +VRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNE
Sbjct: 397 NVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNE 456

Query: 450 LSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGIRPAPFMAAFSS 509
           LSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGIRPAPFMAAFSS
Sbjct: 457 LSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGIRPAPFMAAFSS 516

Query: 510 VGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGI 569
           VGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGI
Sbjct: 517 VGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGI 576

Query: 570 AGLLRTLYPHWSPAAIKSAIMTT--------------------------GHIHPNRATDP 629
           AGLLRTLYPHWSPAAIKSAIMTT                          GHIHPNRATDP
Sbjct: 577 AGLLRTLYPHWSPAAIKSAIMTTASTLDNNFKPLLNASYSVATPFNYGAGHIHPNRATDP 636

Query: 630 GLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITI 689
           GLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITI
Sbjct: 637 GLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITI 696

Query: 690 TRRLKNVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGRKKNY 727
           TRRLKNVGSPGTYKA+IRKPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGRKKNY
Sbjct: 697 TRRLKNVGSPGTYKAKIRKPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGRKKNY 756

BLAST of Cp4.1LG15g06720 vs. NCBI nr
Match: XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])

HSP 1 Score: 1266 bits (3277), Expect = 0.0
Identity = 651/774 (84.11%), Postives = 682/774 (88.11%), Query Frame = 0

Query: 1   MKQPTHPSATVLL--LLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLR 60
           M Q T  SAT LL  LLLSS LLTPT+AIKRSYVVYMGAHSHGGRKPA++VA+SH   LR
Sbjct: 1   MTQTTISSATFLLPFLLLSSSLLTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHKFLR 60

Query: 61  PYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSW 120
           P+L+  ++F EDVIFYSYTRHINGFAAMLEDEVAA+LA+HPKVVS+F N+GR+LHTTRSW
Sbjct: 61  PFLKR-DDFTEDVIFYSYTRHINGFAAMLEDEVAAQLAKHPKVVSIFLNKGRKLHTTRSW 120

Query: 121 EFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGIC 180
           EFM +ENN+GVI+SESIWKKARFGEDTIIGNLDTGVWPESKSFSD DDLGPIP RWRGIC
Sbjct: 121 EFMGMENNNGVITSESIWKKARFGEDTIIGNLDTGVWPESKSFSD-DDLGPIPLRWRGIC 180

Query: 181 QNQNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGA 240
           QNQNDPSFHCNR      YFNKGY+SVVG LNSSFNSPRD EGHGSHTLSTAGGNFVAGA
Sbjct: 181 QNQNDPSFHCNRKLIGARYFNKGYESVVGPLNSSFNSPRDKEGHGSHTLSTAGGNFVAGA 240

Query: 241 SVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGG 300
           SVFGLG G AKGGSPRARVAAYKVCWPPAAGNECFDADILAAFD AIHDGVDVLSVSLGG
Sbjct: 241 SVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDSAIHDGVDVLSVSLGG 300

Query: 301 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSR 360
           DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGP AG+VTNIAPWQITVGASTMDRKFPS 
Sbjct: 301 DPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPAAGTVTNIAPWQITVGASTMDRKFPSL 360

Query: 361 VVLGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKI 420
           VVLGNKKQIE             FYPL+SAADVRLANASAHEAQLCKAGTLDP KAKGKI
Sbjct: 361 VVLGNKKQIEGESLSQNALPSRKFYPLVSAADVRLANASAHEAQLCKAGTLDPMKAKGKI 420

Query: 421 LVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAY 480
           LVCLRGDNARVDKGEQA+LAGAVGMILANNE SGNEILADPHVLPASHINFTDG AV  Y
Sbjct: 421 LVCLRGDNARVDKGEQALLAGAVGMILANNEPSGNEILADPHVLPASHINFTDGTAVLTY 480

Query: 481 INSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGA 540
           INSTKFP+AYIT ATTQLGIRPAPFMAAFSSVGP+TI PEILKPDVTAPGLSVIAAYT A
Sbjct: 481 INSTKFPKAYITRATTQLGIRPAPFMAAFSSVGPNTIIPEILKPDVTAPGLSVIAAYTEA 540

Query: 541 EGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT------ 600
           EGPTNQEYD RR+ FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT      
Sbjct: 541 EGPTNQEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTANTLDN 600

Query: 601 --------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFS 660
                               GH+HPN ATDPGL+YDI VNEYL FLCALGYN+AQ+SQFS
Sbjct: 601 NFEPLLNASYSVATPLNYGAGHVHPNGATDPGLIYDIEVNEYLLFLCALGYNQAQISQFS 660

Query: 661 NGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKP 720
           +GPFNCSEPI+LTNLNYPSITVP LSRSITITRRLKNVGSPGTYKA+IRKPAGISV VKP
Sbjct: 661 SGPFNCSEPINLTNLNYPSITVPELSRSITITRRLKNVGSPGTYKAQIRKPAGISVWVKP 720

Query: 721 KKLSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           KKL+FTRLGEE SFKVLMKVKE N   KKNYVYGDLIWSDGKHHVRSPIVV  V
Sbjct: 721 KKLNFTRLGEEQSFKVLMKVKEQNAA-KKNYVYGDLIWSDGKHHVRSPIVVKVV 771

BLAST of Cp4.1LG15g06720 vs. ExPASy TrEMBL
Match: A0A6J1GL32 (subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111455314 PE=3 SV=1)

HSP 1 Score: 1421 bits (3678), Expect = 0.0
Identity = 719/772 (93.13%), Postives = 727/772 (94.17%), Query Frame = 0

Query: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60
           MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVA+SHRDLLRPY
Sbjct: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVADSHRDLLRPY 60

Query: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120
           LESGNEFAEDVIFYSYTRHINGFAAMLED+VAARLAQHP+VVSVFPNRGRRLHTTRSWEF
Sbjct: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDQVAARLAQHPEVVSVFPNRGRRLHTTRSWEF 120

Query: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180
           MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSD+DDLGPIPQRWRGICQN
Sbjct: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 180

Query: 181 QNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           QNDPSFHCNR      YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 181 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240

Query: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300
           FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP
Sbjct: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360
           NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV
Sbjct: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360

Query: 361 LGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420
           LGNKKQIE             FYPLMSAA+VRLANASAHEAQLCKAGTLDPKKAKGKILV
Sbjct: 361 LGNKKQIEGESLTPEGLPSRKFYPLMSAANVRLANASAHEAQLCKAGTLDPKKAKGKILV 420

Query: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480
           CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAY+N
Sbjct: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYVN 480

Query: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540
           STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG
Sbjct: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540

Query: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT-------- 600
           PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAI+TT        
Sbjct: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIVTTASTLDNNF 600

Query: 601 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660
                             GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG
Sbjct: 601 KPLLNASYSVATPFNYGAGHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660

Query: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720
           PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKA+IRKPAGISVSVKPKK
Sbjct: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAKIRKPAGISVSVKPKK 720

Query: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV
Sbjct: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 772

BLAST of Cp4.1LG15g06720 vs. ExPASy TrEMBL
Match: A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)

HSP 1 Score: 1404 bits (3633), Expect = 0.0
Identity = 709/772 (91.84%), Postives = 722/772 (93.52%), Query Frame = 0

Query: 1   MKQPTHPSATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPY 60
           MKQPT+PS T LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPA+IVA+SHR+LLRPY
Sbjct: 23  MKQPTYPSTTFLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPADIVADSHRELLRPY 82

Query: 61  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEF 120
           LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLA+HP+VVSVFPNRGRRLHTTRSWEF
Sbjct: 83  LESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAKHPEVVSVFPNRGRRLHTTRSWEF 142

Query: 121 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQN 180
           MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSD+DDLGPIPQRWRGICQN
Sbjct: 143 MELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDYDDLGPIPQRWRGICQN 202

Query: 181 QNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 240
           QNDPSFHCNR      YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV
Sbjct: 203 QNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASV 262

Query: 241 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 300
           FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP
Sbjct: 263 FGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDP 322

Query: 301 NPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 360
           NPLFNDSVAIGSFHAV+HGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV
Sbjct: 323 NPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVV 382

Query: 361 LGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 420
           LGNKKQIE             FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV
Sbjct: 383 LGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILV 442

Query: 421 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 480
           CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN
Sbjct: 443 CLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN 502

Query: 481 STKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEG 540
           STKFPQAYITGATTQLG RPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYT AEG
Sbjct: 503 STKFPQAYITGATTQLGTRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTEAEG 562

Query: 541 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT-------- 600
           PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT        
Sbjct: 563 PTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNF 622

Query: 601 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 660
                             GHIHPN+ATDPGLVYDIT+ EYLSFLCALGYNKAQLSQFSNG
Sbjct: 623 KPLLNASYSVATPFNYGAGHIHPNKATDPGLVYDITITEYLSFLCALGYNKAQLSQFSNG 682

Query: 661 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKK 720
           PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIR+PAGISVSVKPKK
Sbjct: 683 PFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRRPAGISVSVKPKK 742

Query: 721 LSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           LSFTRLGEELSFKVLMKVKEGNVGR+KNYVYGDLIWSDGKHHVRSPIVVMA+
Sbjct: 743 LSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDLIWSDGKHHVRSPIVVMAI 794

BLAST of Cp4.1LG15g06720 vs. ExPASy TrEMBL
Match: A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 641/775 (82.71%), Postives = 680/775 (87.74%), Query Frame = 0

Query: 1   MKQPTHPSATVLL---LLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLL 60
           MK  T  S+  LL   LLLSS L TPT+AIKRSYVVYMGAHSHGGRKPA++VA+SH   L
Sbjct: 1   MKLSTLSSSIFLLSLFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFL 60

Query: 61  RPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRS 120
           +P+L+SG EF +DVIFYSYTRHINGFAAMLEDEVAA+LA+HP+VVSVF NRGR+LHTTRS
Sbjct: 61  QPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRS 120

Query: 121 WEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGI 180
           WEFM LEN +GVI SESIWKKARFGEDTIIGNLDTGVW ESKSFSD D++GPIP RW+GI
Sbjct: 121 WEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGVWAESKSFSD-DEMGPIPHRWKGI 180

Query: 181 CQNQNDPSFHCNR------YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAG 240
           CQNQNDPSFHCNR      YFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAG
Sbjct: 181 CQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAG 240

Query: 241 ASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLG 300
           ASVFGLGKG AKGGSPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLSVSLG
Sbjct: 241 ASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLG 300

Query: 301 GDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPS 360
           GDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS
Sbjct: 301 GDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPS 360

Query: 361 RVVLGNKKQIE-------------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGK 420
            VVLGNKKQIE              YPLM+AADVRLANASAHEAQLCKAGTLDP KAKGK
Sbjct: 361 LVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGK 420

Query: 421 ILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFA 480
           ILVCLRGDNARVDKGEQA+LAGA GMILANNELSGNEILADPHVLPA HIN+TDG AVFA
Sbjct: 421 ILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFA 480

Query: 481 YINSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTG 540
           YINSTKFP+AYIT ATTQLGIRPAPFMAAFSSVGP+T+TPEILKPD+TAPGLSVIAAYT 
Sbjct: 481 YINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTE 540

Query: 541 AEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT----- 600
           AEGPTNQE+D RRV FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT     
Sbjct: 541 AEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLD 600

Query: 601 ---------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQF 660
                                GH+HPN ATDPGLVYDI VNEYLSFLCALGY+KAQ+SQF
Sbjct: 601 NNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQF 660

Query: 661 SNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVK 720
           S+GPFNCSEPISLTNLNYPSITVP LSRSITITRRLKNVGSPGTY+A+IRKPAGISV VK
Sbjct: 661 SDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVK 720

Query: 721 PKKLSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDLIWSDGKHHVRSPIVVMAV 727
           PKKL+FT+LGEELSFKV MKVK G+   KKNYVYGDLIWSDGKHHVRSPIVV  V
Sbjct: 721 PKKLNFTKLGEELSFKVFMKVK-GHKVAKKNYVYGDLIWSDGKHHVRSPIVVKVV 773

BLAST of Cp4.1LG15g06720 vs. ExPASy TrEMBL
Match: A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)

HSP 1 Score: 1256 bits (3250), Expect = 0.0
Identity = 636/761 (83.57%), Postives = 674/761 (88.57%), Query Frame = 0

Query: 12  LLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPYLESGNEFAEDV 71
           L LLLSS L TPT+AIKRSYVVYMGAHSHGGRKPA++VA+SH   L+P+L+SG EF +DV
Sbjct: 15  LFLLLSSSLFTPTSAIKRSYVVYMGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDV 74

Query: 72  IFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVIS 131
           IFYSYTRHINGFAAMLEDEVAA+LA+HP+VVSVF NRGR+LHTTRSWEFM LEN +GVI 
Sbjct: 75  IFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIE 134

Query: 132 SESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCNR- 191
           SESIWKKARFGEDTIIGNLDTGVW ESKSFSD D++GPIP RW+GICQNQNDPSFHCNR 
Sbjct: 135 SESIWKKARFGEDTIIGNLDTGVWAESKSFSD-DEMGPIPHRWKGICQNQNDPSFHCNRK 194

Query: 192 -----YFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGG 251
                YFNKGY SVVG LNSSF+SPRD EGHGSHTLSTAGGNFVAGASVFGLGKG AKGG
Sbjct: 195 LIGARYFNKGYASVVGPLNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGG 254

Query: 252 SPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIG 311
           SPRARVAAYKVCWPP AGNECFDADILAAFD+AIHDGVDVLSVSLGGDPNPLFNDSVAIG
Sbjct: 255 SPRARVAAYKVCWPPKAGNECFDADILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIG 314

Query: 312 SFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIE--- 371
           SFHA+KHGIVVICSAGNSGP AG+VTN+APWQITVGASTMDRKFPS VVLGNKKQIE   
Sbjct: 315 SFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEGES 374

Query: 372 ----------FYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDK 431
                      YPLM+AADVRLANASAHEAQLCKAGTLDP KAKGKILVCLRGDNARVDK
Sbjct: 375 LSQEALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDK 434

Query: 432 GEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITG 491
           GEQA+LAGA GMILANNELSGNEILADPHVLPA HIN+TDG AVFAYINSTKFP+AYIT 
Sbjct: 435 GEQALLAGAAGMILANNELSGNEILADPHVLPALHINYTDGSAVFAYINSTKFPEAYITP 494

Query: 492 ATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRV 551
           ATTQLGIRPAPFMAAFSSVGP+T+TPEILKPD+TAPGLSVIAAYT AEGPTNQE+D RRV
Sbjct: 495 ATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRV 554

Query: 552 AFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT------------------- 611
            FNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT                   
Sbjct: 555 PFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVA 614

Query: 612 -------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLT 671
                  GH+HPN ATDPGLVYDI VNEYLSFLCALGY+KAQ+SQFS+GPFNCSEPISLT
Sbjct: 615 TPFNYGAGHVHPNGATDPGLVYDIDVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLT 674

Query: 672 NLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGEELS 727
           NLNYPSITVP LSRSITITRRLKNVGSPGTY+A+IRKPAGISV VKPKKL+FT+LGEELS
Sbjct: 675 NLNYPSITVPKLSRSITITRRLKNVGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELS 734

BLAST of Cp4.1LG15g06720 vs. ExPASy TrEMBL
Match: A0A0A0K313 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1)

HSP 1 Score: 1226 bits (3171), Expect = 0.0
Identity = 617/738 (83.60%), Postives = 651/738 (88.21%), Query Frame = 0

Query: 35  MGAHSHGGRKPANIVANSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAAR 94
           MGAHSHGG+KPA++VANSH + L+P+L+SG EF +DVIFYSYTRHINGFAAMLEDEVA +
Sbjct: 1   MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 60

Query: 95  LAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGV 154
           LA+HPKVVSVF NRGR+LHTTRSWEFM LEN +GVI+SESIWKKARFGEDTIIGNLDTGV
Sbjct: 61  LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 120

Query: 155 WPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCNR------YFNKGYKSVVGHLNSSFN 214
           W ESKSFSD D+ GPIP RW+GICQNQ DPSFHCNR      YFNKGY SVVG LNSSF+
Sbjct: 121 WAESKSFSD-DEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFH 180

Query: 215 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFD 274
           SPRD EGHGSHTLSTAGGNFVAGASVFGLGKG AKGGSPRARVAAYKVCWPP AGNECFD
Sbjct: 181 SPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFD 240

Query: 275 ADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAG 334
           ADILAAFD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGP AG
Sbjct: 241 ADILAAFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAG 300

Query: 335 SVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIE-------------FYPLMSAADVRLA 394
           +VTN+APWQITVGASTMDRKFPS VVLGN+KQIE              YPLM+AADVRLA
Sbjct: 301 TVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLA 360

Query: 395 NASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNE 454
           NAS HEAQLCKAGTL+P KAKGKILVCLRGDNARVDKGEQA+LAGA GMILANNELSGNE
Sbjct: 361 NASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNE 420

Query: 455 ILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPST 514
           ILADPHVLPASHINFTDG AVFAYINSTK+P+AYIT ATTQLGIRPAPFMAAFSSVGP+T
Sbjct: 421 ILADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNT 480

Query: 515 ITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLR 574
           +TPEILKPD+TAPGLSVIAAYT AEGPTNQE+D RR+ FNSVSGTSMSCPHVSGIAGLL+
Sbjct: 481 VTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLK 540

Query: 575 TLYPHWSPAAIKSAIMTT--------------------------GHIHPNRATDPGLVYD 634
           TLYPHWSPAAIKSAIMTT                          GH+HPN A DPGLVYD
Sbjct: 541 TLYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYD 600

Query: 635 ITVNEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLK 694
           I VNEYLSFLCALGYNKAQ+SQFSNGPFNCS+PIS TNLNYPSITVP LSRSITITRRLK
Sbjct: 601 IEVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLK 660

Query: 695 NVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGRKKNYVYGDL 727
           NVGSPGTYKAEIRKPAGISV VKPKKLSFTRLGEELSFKVLMKVKE  V  KKNYVYGDL
Sbjct: 661 NVGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVA-KKNYVYGDL 720

BLAST of Cp4.1LG15g06720 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 896.0 bits (2314), Expect = 2.0e-260
Identity = 458/772 (59.33%), Postives = 562/772 (72.80%), Query Frame = 0

Query: 5   THPSATVLLLLLSSLLLTPTAAIK--RSYVVYMGAHSHGG---RKPANIVANSHRDLLRP 64
           TH  + +LLLLL  +      A K   SYVVY GAHSH G       + V  +H D L  
Sbjct: 4   THNFSFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGS 63

Query: 65  YLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWE 124
           +  S  E A D IFYSYT+HINGFAA L+ ++A  +++HP+VVSVFPN+  +LHTTRSW+
Sbjct: 64  FTGS-RERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWD 123

Query: 125 FMELENNHGVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQ 184
           F+ LE+N   + S SIW+KARFGEDTII NLDTGVWPESKSF D + LGPIP RW+GICQ
Sbjct: 124 FLGLEHN-SYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRD-EGLGPIPSRWKGICQ 183

Query: 185 NQNDPSFHCN------RYFNKGYKSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGAS 244
           NQ D +FHCN      RYFNKGY + VGHLNSSF+SPRD +GHGSHTLSTA G+FV G S
Sbjct: 184 NQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVS 243

Query: 245 VFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGD 304
           +FG G G AKGGSPRARVAAYKVCWPP  GNEC+DAD+LAAFD AIHDG DV+SVSLGG+
Sbjct: 244 IFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGE 303

Query: 305 PNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRV 364
           P   FNDSVAIGSFHA K  IVV+CSAGNSGP   +V+N+APWQITVGASTMDR+F S +
Sbjct: 304 PTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNL 363

Query: 365 VLGNKK-------------QIEFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKIL 424
           VLGN K               +FYP+M++ + +  NASA +AQLCK G+LDP K KGKIL
Sbjct: 364 VLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKIL 423

Query: 425 VCLRGDNARVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYI 484
           VCLRG N RV+KG    L G +GM+L N  ++GN++LADPHVLPA+ +   D  AV  YI
Sbjct: 424 VCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYI 483

Query: 485 NSTKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAE 544
           + TK P A+IT + T LG++PAP MA+FSS GPS + P+ILKPD+TAPG+SVIAAYTGA 
Sbjct: 484 SQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAV 543

Query: 545 GPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT------- 604
            PTN+++D RR+ FN++SGTSMSCPH+SGIAGLL+T YP WSPAAI+SAIMTT       
Sbjct: 544 SPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDI 603

Query: 605 -------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSN 664
                              GH+ PN A +PGLVYD+ + +YL+FLC+LGYN +Q+S FS 
Sbjct: 604 PGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSG 663

Query: 665 GPFNCSEP-ISLTNLNYPSITVPNLSRS-ITITRRLKNVGSPGTYKAEIRKPAGISVSVK 724
             F CS P ISL NLNYPSITVPNL+ S +T++R +KNVG P  Y  ++  P G+ V+VK
Sbjct: 664 NNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVK 723

BLAST of Cp4.1LG15g06720 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 823.2 bits (2125), Expect = 1.7e-238
Identity = 424/761 (55.72%), Postives = 540/761 (70.96%), Query Frame = 0

Query: 13  LLLLSSLLLTPTAAIKRSYVVYMGAHSHGGR-KPANI--VANSHRDLLRPYLESGNEFAE 72
           LLLL +L  +P  A+K+SY+VY+G+H+H  +   A++  VA+SHR  L  ++ S +E A+
Sbjct: 24  LLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGS-HENAK 83

Query: 73  DVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGV 132
           + IFYSY RHINGFAA+L++  AA +A+HP VVSVFPN+GR+LHTT SW FM L  N GV
Sbjct: 84  EAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN-GV 143

Query: 133 ISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQNQNDPSFHCN 192
           +   S+W KA +GEDTII NLDTGVWPESKSFSD +  G +P RW+G C         CN
Sbjct: 144 VHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSD-EGYGAVPARWKGRCHK----DVPCN 203

Query: 193 ------RYFNKGYKSVVG-HLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIA 252
                 RYFNKGY +  G   N+S+ + RD++GHGSHTLSTA GNFV GA+VFG+G G A
Sbjct: 204 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 263

Query: 253 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSV 312
            GGSP+ARVAAYKVCWPP  G ECFDADILAA + AI DGVDVLS S+GGD     +D +
Sbjct: 264 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 323

Query: 313 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQI- 372
           AIGSFHAVK+G+ V+CSAGNSGP +G+V+N+APW ITVGAS+MDR+F + V L N +   
Sbjct: 324 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 383

Query: 373 -----------EFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARV 432
                      + Y L+SAAD  +AN +  +A LCK G+LDPKK KGKILVCLRGDNARV
Sbjct: 384 GTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARV 443

Query: 433 DKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYI 492
           DKG QA  AGA GM+L N++ SGNEI++D HVLPAS I++ DG  +F+Y++STK P+ YI
Sbjct: 444 DKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYI 503

Query: 493 TGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKR 552
              T  L  +PAPFMA+FSS GP+TITP ILKPD+TAPG+++IAA+T A GPT+ + D R
Sbjct: 504 KAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNR 563

Query: 553 RVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT----------------- 612
           R  FN+ SGTSMSCPH+SG+ GLL+TL+PHWSPAAI+SAIMTT                 
Sbjct: 564 RTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFK 623

Query: 613 ---------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGP-FNCSEPI 672
                    GH+ PN+A  PGLVYD+T  +YL FLCA+GYN   +  F+  P + C +  
Sbjct: 624 KANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGA 683

Query: 673 SLTNLNYPSITVPNLSRSITITRRLKNVGSPGTYKAEIRKPAGISVSVKPKKLSFTRLGE 725
           +L + NYPSITVPNL+ SIT+TR+LKNVG P TY A  R+P G+ VSV+PK+L+F + GE
Sbjct: 684 NLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGE 743

BLAST of Cp4.1LG15g06720 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 548.1 bits (1411), Expect = 1.0e-155
Identity = 323/767 (42.11%), Postives = 455/767 (59.32%), Query Frame = 0

Query: 8   SATVLLLLLSSLLLTPTAAIKRSYVVYMGAHSHGGRKPANIVANSHRDLLRPYLESGNEF 67
           +A  LLL L    ++ +++ + +Y+V+M       + P++   + H +     L S ++ 
Sbjct: 9   TAFFLLLCLGFCHVSSSSSDQGTYIVHMAK----SQMPSSF--DLHSNWYDSSLRSISDS 68

Query: 68  AEDVIFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNH 127
           AE  + Y+Y   I+GF+  L  E A  L   P V+SV P     LHTTR+  F+ L+ + 
Sbjct: 69  AE--LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH- 128

Query: 128 GVISSESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGICQ-NQNDPSF 187
               +  ++ +A    D ++G LDTGVWPESKS+SD +  GPIP  W+G C+   N  + 
Sbjct: 129 ----TADLFPEAGSYSDVVVGVLDTGVWPESKSYSD-EGFGPIPSSWKGGCEAGTNFTAS 188

Query: 188 HCN------RYFNKGYKSVVGHLNSS--FNSPRDNEGHGSHTLSTAGGNFVAGASVFGLG 247
            CN      R+F +GY+S +G ++ S    SPRD++GHG+HT STA G+ V GAS+ G  
Sbjct: 189 LCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYA 248

Query: 248 KGIAKGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLF 307
            G A+G +PRARVA YKVCW       CF +DILAA D AI D V+VLS+SLGG  +  +
Sbjct: 249 SGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYY 308

Query: 308 NDSVAIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNK 367
            D VAIG+F A++ GI+V CSAGN+GP++ S++N+APW  TVGA T+DR FP+  +LGN 
Sbjct: 309 RDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNG 368

Query: 368 KQI--------EFYPLMSAADVRLANAS-AHEAQLCKAGTLDPKKAKGKILVCLRGDNAR 427
           K          E  P      +   NAS A    LC  GTL P+K KGKI++C RG NAR
Sbjct: 369 KNFTGVSLFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINAR 428

Query: 428 VDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAY 487
           V KG+    AG VGMILAN   +G E++AD H+LPA+ +    G  +  Y+ +   P A 
Sbjct: 429 VQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTAS 488

Query: 488 ITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDK 547
           I+   T +G++P+P +AAFSS GP++ITP ILKPD+ APG++++AA+TGA GPT    D 
Sbjct: 489 ISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDS 548

Query: 548 RRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT---------------- 607
           RRV FN +SGTSMSCPHVSG+A LL++++P WSPAAI+SA+MTT                
Sbjct: 549 RRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIAT 608

Query: 608 -----------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGPFNC--S 667
                      GH+ P  AT+PGL+YD+T  +YL FLCAL Y   Q+   S   + C  S
Sbjct: 609 GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPS 668

Query: 668 EPISLTNLNYPSITVPNLS--RSITITRRLKNVGSPGTYKAEI-RKPAGISVSVKPKKLS 725
           +  S+ +LNYPS  V N+    +   TR + +VG  GTY  ++  +  G+ +SV+P  L+
Sbjct: 669 KSYSVADLNYPSFAV-NVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLN 728

BLAST of Cp4.1LG15g06720 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 541.6 bits (1394), Expect = 9.8e-154
Identity = 311/719 (43.25%), Postives = 425/719 (59.11%), Query Frame = 0

Query: 72  IFYSYTRHINGFAAMLEDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVIS 131
           I ++Y    +GF+A L  + A++L  HP V+SV P + R LHTTRS EF+ L +      
Sbjct: 62  IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRST----D 121

Query: 132 SESIWKKARFGEDTIIGNLDTGVWPESKSFSDHDDLGPIPQRWRGIC-QNQNDPSFHCN- 191
              + +++ FG D +IG +DTGVWPE  SF D   LGP+P +W+G C  +Q+ P   CN 
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDR-GLGPVPIKWKGQCIASQDFPESACNR 181

Query: 192 -----RYFNKGYKSVVGHLN--SSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIA 251
                R+F  GY++  G +N  + F SPRD++GHG+HT S + G +V  AS  G   G+A
Sbjct: 182 KLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVA 241

Query: 252 KGGSPRARVAAYKVCWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSV 311
            G +P+AR+AAYKVCW     + C+D+DILAAFD A+ DGVDV+S+S+GG   P + D++
Sbjct: 242 AGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAI 301

Query: 312 AIGSFHAVKHGIVVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQI- 371
           AIG+F A+  GI V  SAGN GP A +VTN+APW  TVGA T+DR FP+ V LGN K I 
Sbjct: 302 AIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMIS 361

Query: 372 -------------EFYPLMSAADVRLANASAHEAQLCKAGTLDPKKAKGKILVCLRGDNA 431
                          YPL+      L     + + LC  G+LDP   KGKI++C RG N+
Sbjct: 362 GVSVYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINS 421

Query: 432 RVDKGEQAMLAGAVGMILANNELSGNEILADPHVLPASHINFTDGVAVFAYIN------S 491
           R  KGE     G +GMI+AN    G  ++AD HVLPA+ +  + G  +  YI+      S
Sbjct: 422 RATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRS 481

Query: 492 TKFPQAYITGATTQLGIRPAPFMAAFSSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGP 551
           +K P A I    T+LGIRPAP +A+FS+ GP+  TPEILKPDV APGL+++AA+    GP
Sbjct: 482 SKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGP 541

Query: 552 TNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLRTLYPHWSPAAIKSAIMTT--------- 611
           +    D RR  FN +SGTSM+CPHVSG+A LL+  +P WSPAAI+SA++TT         
Sbjct: 542 SGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGE 601

Query: 612 ------------------GHIHPNRATDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNG 671
                             GH+HP +A DPGLVYDIT  +Y++FLC   Y +  +   +  
Sbjct: 602 PMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRR 661

Query: 672 PFNCS---EPISLTNLNYPSITV-----PNLSRSITITRRLKNVG-SPGTYKAEIRKPAG 725
             +C        + NLNYPS +V          S    R + NVG S   Y+ +IR P G
Sbjct: 662 QADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRG 721

BLAST of Cp4.1LG15g06720 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 533.9 bits (1374), Expect = 2.0e-151
Identity = 320/754 (42.44%), Postives = 438/754 (58.09%), Query Frame = 0

Query: 30  SYVVYMGAHSHGGRKPANIVA--NSHRDLLRPYLESGNEFAEDVIFYSYTRHINGFAAML 89
           SY+V    H     KP+   +  N H  LLR  L S  + A   + YSY+R ++GF+A L
Sbjct: 32  SYIV----HVQRSHKPSLFSSHNNWHVSLLRS-LPSSPQPA--TLLYSYSRAVHGFSARL 91

Query: 90  EDEVAARLAQHPKVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTII 149
                A L +HP V+SV P++ R +HTT +  F+    N G      +W  + +GED I+
Sbjct: 92  SPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSG------LWSNSNYGEDVIV 151

Query: 150 GNLDTGVWPESKSFSDHDDLGPIPQRWRGICQNQND-PSFHCN------RYFNKGY---- 209
           G LDTG+WPE  SFSD   LGPIP  W+G C+   D P+  CN      R F +GY    
Sbjct: 152 GVLDTGIWPEHPSFSD-SGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQR 211

Query: 210 KSVVGHLNSSFNSPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKV 269
                H      SPRD EGHG+HT STA G+ VA AS++   +G A G + +AR+AAYK+
Sbjct: 212 NGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKI 271

Query: 270 CWPPAAGNECFDADILAAFDVAIHDGVDVLSVSLG--GDPNPLFNDSVAIGSFHAVKHGI 329
           CW       C+D+DILAA D A+ DGV V+S+S+G  G       DS+AIG+F A +HGI
Sbjct: 272 CWT----GGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGI 331

Query: 330 VVICSAGNSGPTAGSVTNIAPWQITVGASTMDRKFPSRVVLGNKKQI---EFYPLMSAAD 389
           VV CSAGNSGP   + TNIAPW +TVGAST+DR+F +  + G+ K       Y   S  D
Sbjct: 332 VVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPD 391

Query: 390 VRLANASAHE--AQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANN 449
            +L+   + +  ++LC  G L+    +GKI++C RG NARV+KG    LAG  GMILAN 
Sbjct: 392 SQLSLVYSGDCGSRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANT 451

Query: 450 ELSGNEILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLG-IRPAPFMAAF 509
             SG E+ AD H++PA+ +    G  +  YI ++  P A I+   T +G   P+P +AAF
Sbjct: 452 AESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAF 511

Query: 510 SSVGPSTITPEILKPDVTAPGLSVIAAYTGAEGPTNQEYDKRRVAFNSVSGTSMSCPHVS 569
           SS GP+ +TP ILKPDV APG++++A +TG  GPT+ + D RRV FN +SGTSMSCPHVS
Sbjct: 512 SSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVS 571

Query: 570 GIAGLLRTLYPHWSPAAIKSAIMTT---------------------------GHIHPNRA 629
           G+A LLR  +P WSPAAIKSA++TT                           GH+ PN+A
Sbjct: 572 GLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKA 631

Query: 630 TDPGLVYDITVNEYLSFLCALGYNKAQLSQFSNGP--FNCSEPISLT---NLNYPSITV- 689
            +PGLVYDI V EY++FLCA+GY    +  F   P  ++  E   L    +LNYPS +V 
Sbjct: 632 LNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVV 691

Query: 690 -PNLSRSITITRRLKNVGS--PGTYKAEIRKPAGISVSVKPKKLSFTRLGEELSFKVLMK 725
             +    +   R +KNVGS     Y+  ++ PA + + V P KL+F++    L ++V  K
Sbjct: 692 FASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFK 751

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSP52.9e-25959.33Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC52.4e-23755.72Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4621.6e-17746.34Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653511.5e-15442.11Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
Q9LUM31.4e-15243.25Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... [more]
Match NameE-valueIdentityDescription
XP_023554401.10.094.17subtilisin-like protease SBT5.3 [Cucurbita pepo subsp. pepo][more]
XP_022952697.10.093.13subtilisin-like protease SBT5.3 [Cucurbita moschata][more]
XP_022968976.10.091.84subtilisin-like protease SBT5.3 isoform X1 [Cucurbita maxima][more]
KAG6572205.10.093.27Subtilisin-like protease 5.3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038888965.10.084.11subtilisin-like protease SBT5.3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GL320.093.13subtilisin-like protease SBT5.3 OS=Cucurbita moschata OX=3662 GN=LOC111455314 PE... [more]
A0A6J1HWD20.091.84subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3BUG60.082.71Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CG030.083.57subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035004... [more]
A0A0A0K3130.083.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G04160.12.0e-26059.33Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.11.7e-23855.72Subtilase family protein [more]
AT5G67360.11.0e-15542.11Subtilase family protein [more]
AT3G14240.19.8e-15443.25Subtilase family protein [more]
AT3G14067.12.0e-15142.44Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 212..225
score: 32.92
coord: 538..554
score: 54.67
coord: 142..161
score: 27.57
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 142..574
e-value: 3.3E-40
score: 138.7
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 144..595
e-value: 1.2E-159
score: 534.0
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 128..579
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 18..113
e-value: 6.2E-22
score: 79.8
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 627..724
e-value: 2.5E-28
score: 98.2
NoneNo IPR availableGENE3D3.50.30.30coord: 346..477
e-value: 1.2E-159
score: 534.0
NoneNo IPR availableGENE3D2.60.40.2310coord: 599..726
e-value: 3.3E-38
score: 132.3
NoneNo IPR availablePANTHERPTHR43806PEPTIDASE S8coord: 16..726
NoneNo IPR availablePANTHERPTHR43806:SF38SUBTILISIN-LIKE PROTEASE SBT5.4coord: 16..726
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 117..594
score: 25.264626
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 353..470
e-value: 1.71713E-35
score: 128.685
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 392..479
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 30..113
e-value: 1.3E-13
score: 51.4
IPR003137PA domainPFAMPF02225PAcoord: 383..454
e-value: 5.4E-10
score: 39.2
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 539..549
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 112..573
e-value: 2.17252E-126
score: 376.553

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g06720.1Cp4.1LG15g06720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity