Cp4.1LG15g05780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g05780
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Glutamyl-tRNA(Gln) amidotransferase subunit A
Location	Cp4.1LG15: 6627809 .. 6631564 (+)
RNA-Seq Expression	Cp4.1LG15g05780
Synteny	Cp4.1LG15g05780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCAACGTTCTGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACCATACCTGACCTTCAGCTAGCTTTCAAGCGAAACCAGCTCACTTCGACGGGCCTTGTCGAGTTCTACATTGGGGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGAGTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTGTTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTACCCCGTGATGCAGGCGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCCCTTGCTGCTCCTGCTGGTTTGAGCGCTAGAGGTGGCCAGGGAAAGGTGTGTTGCTTTGAATTTAGGTTCCTACGTAACTAAAAACCTATAGCAACTCAAGTCACGGCTAGCCGATATTATCCTCTTTAGGTTTTCCCTTCCAGGCGTCCTCTCAAGGTTTTAAAATGTGTCCACTAGGGAGAGGTTTCCACACTCTTATAAGGAATGTTTCGTTTTGTTCCCCTCTCCAACCGATATGTGATCTCACAATTCACCCCTTCAGAGACCCAGCGTCCTCGTTGGCACAGCCCCTGGTATCTAGCTCTGACCATTTGTAACAATCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGACTTTTTCCTTCTGGGCTTCCCCTCAAGTTTTAAAACATATCCACTAGGGAGATGTTTCCACACTTTTATAAGGAATATTTCGTTTTGTTCCCCTCTCCAACCGATGTGGGATCTCAGAATCCACCCCTTAGGAGGCCAGCATTCTCGTTGGCACACCGCCTGGTGTCTATCTCTGACCATTTGTAACAGTCCAAGTCCACTGCTAGTAGATATTGTCCTCTTTAGGTTTTCCCTTCCGGGCTTCCACTCGAAGTTTTAAAACGCGTCCACTATGGAGAGATTCCCACACCCTTATAAGGAATGTTTCGTTCCCCTTTCCAACCGATGTGGGATATCAGAATCCACTCCCCTTGAGAACCCAGCGTCCTCATTAGCACACCGCCCAGTGTCTGGCTCTGATACCATTTATAACAGCTCAAACTCACCGCTAGCAGACATTGTCCTCTCTAGGCTTTCCCTTTTCGGCTTCCCCTCAATGGTTTTAAATCGCGTCCACTAGGGAGAGGATTCCACACCTTTAAAAGCAATGTTTCGTTCCTCTCTCCAACCGATATGGGATCTCACGAAACCATTTTTTTTGTACCATTTTTTTTTTCAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTAGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCGACCGTCGGCCTCACCAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATAGGGTGAGCAATCTGGCATCCATATCAGCAAAACATTCTACACAGATCATAAGAACTGAAACTTTGTAACCTTCACATGTGAAAACAGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTGGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTCTCAGCTTCTTTAATGATTCAGCCATCACTCAAGCTTTTGAGGAACATTTCAACATTCTAAAGTAAGAAAACCAACTTCCCTATCCATGCAAATGCTAATCATTCCATAAACAGGTTCATATTCACTTAAAACAAAAGGCTCGATCCATTAATGTGAGATCTCATATCGGTTGGAGAAGGGAACGAAGTATTGCTCATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTGAAGGGAAGCCTGGAAGGGAAAACCCAAAAAGAATAATATCTGCTAGCGGTGGGCTTAGGCTGTTACAAATGATATCAAAACCAGACATCAGGCGGTGTGCTAGCAAGGACGCTGGGCTCCCAAGGGGGTGGATTGTAAGATCCCACATCGGTTTGAGAGGGGAATGAAACATTTCTTATAAGGGTGTGGAAACTTCTCCCTAGTAGACACGTTGGTGGATTGTGAGATCCCAAATCAGTTGGAGAGGGGAACGAAACATTGCTTATAAGGGTGTGGAAACCTCTCCCTCGTAGACGCGTTTTGAAGGGAATCTCGGAAGGGAAAGCCTAAAGAGGACAATATCTGCTAGTGGTGGGCTTGGGCTGTTACAAATAGTATAAGAACCAAACACCAGGCGGTGTGCCAGCGAGGACGTTGGGCCCCAGGAGGGTGGATTGTGAGATCCCACACCGATTAGAGAAGAGAACAAAACATTGCTTATAAGGGTGTGGAAACCTCTCCTTAGTAGACACGGTGGATTGTAAGATCCCACATCGGTTGAAGAGGGGAACGAAACATTGTTTATAATAATGTGAAAACCTCTCTTAATAGATTTACTTATAAGGGTGTGAAAACATCTCCCTAGTGGACTTGATTATAAGGATGTGGAAAACTCTCCCTAGTAGACGCGTTGGTGGATTGTGAGATCCCATATCGGTTGGAGAGGGGAACGAAGCATTGCTTATAAGGGTGTGGGAACCTCTCCCGAGTAGACGTGTTTTAAAACCTTGAAGGGAAGCCTGAAAGAAAGGGAAAACCAAAGAGAACAATATCGGCTAGCAGTGAACTTGGACTCTTCCTTACGAATTAAGAAAAATCAATTCAACAGGAACACAAACAACCAATCTTCTAAAGGAATAGTTTACAAGCCTCTTAACTCAAATTATATTCCATTGTTCTTCTAGTAAACACTAAATCTAACATTGTTAAAACTCAAGGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGCTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGTAACACAATATTCAATCCATTGTTCATCGCATAGTAATCGCATTCATTCGGAATCGATTCGAATCATGGTTGTGCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGGTTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATAGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTCGGAGGGTTGAAGGGTTCAGAGCCGAAGCTGATTGAGGTTGCTTACGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

mRNA sequence

ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCAACGTTCTGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACCATACCTGACCTTCAGCTAGCTTTCAAGCGAAACCAGCTCACTTCGACGGGCCTTGTCGAGTTCTACATTGGGGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGAGTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTGTTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTACCCCGTGATGCAGGCGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGTGGACCTTCCATATCAGTAGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCGACCGTCGGCCTCACCAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATAGGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTGGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTCTCAGCTTCTTTAATGATTCAGCCATCACTCAAGCTTTTGAGGAACATTTCAACATTCTAAAGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGCTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGGTTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATAGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTCGGAGGGTTGAAGGGTTCAGAGCCGAAGCTGATTGAGGTTGCTTACGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

Coding sequence (CDS)

ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCAACGTTCTGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACCATACCTGACCTTCAGCTAGCTTTCAAGCGAAACCAGCTCACTTCGACGGGCCTTGTCGAGTTCTACATTGGGGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGAGTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTGTTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTACCCCGTGATGCAGGCGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGTGGACCTTCCATATCAGTAGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGTTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCGACCGTCGGCCTCACCAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATAGGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTGGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTCTCAGCTTCTTTAATGATTCAGCCATCACTCAAGCTTTTGAGGAACATTTCAACATTCTAAAGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGCAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGCTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCCACAAATGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGGTTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATAGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTCGGAGGGTTGAAGGGTTCAGAGCCGAAGCTGATTGAGGTTGCTTACGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

Protein sequence

MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPKLIEVAYAFEQATLIRKPPSFKP

Homology

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.0e-156
Identity = 298/512 (58.20%), Postives = 379/512 (74.02%), Query Frame = 0


Query: 3   YSSWLLIIV---AFCLTVQRSAAVR---GFSIREATIPDLQLAFKRNQLTSTGLVEFYIG 62
           +SS L++++   A  ++V  ++ +R    FSI+EATI D+++AF   +LTS  LVE Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 63  EIRRLNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNT 122
            I +LNP++H VIE NPDAL+QA  +DRER+ K    L  LHG+PVLLKDSI TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 123 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF------------------- 182
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASLSEWA F                   
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 183 -----------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 242
                      SG +ISV AN+ AVS+GTETDGSIL PAS NSVVGIKP+VGLTSRAGV+
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 243 PVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGK 302
           P+S RQD+IGPI RTV+DAV +LD IVG+D  DEAT+T+S++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 303 RLGIVRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVAL 362
           RLGIV             +   + H   L++ GAI+++NL I NI++I+  T SGE +AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 363 LAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQ 422
           LAEFK SLN YLKELV SPVRSLAD+IA++    +QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 423 KAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFG 479
           K AL  + +L+++G EK++EE +LDA+VT G  +++VLAIGG+PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 6.9e-68
Identity = 186/518 (35.91%), Postives = 265/518 (51.16%), Query Frame = 0


Query: 24  RGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKSD 83
           +  ++ +ATI  LQ   +   LTST +V  Y+    ++NP V+G++++NPD L  A + D
Sbjct: 62  QNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD 121

Query: 84  REREAKKPGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAG 143
            ER     G + G LHGIP ++KD+  TKDK++TTAGS+ALLGSIVPRDA VVK+LR AG
Sbjct: 122 DER---ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 181

Query: 144 AIILGKASLSEWADF------------------------------SGPSISVAANIAAVS 203
           A++ G A+LSEWAD                               SG +ISVA+N+ A +
Sbjct: 182 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 241

Query: 204 IGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTI 263
           +GTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV V  ++
Sbjct: 242 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 301

Query: 264 VGFDYNDEATRTSSKYIPY-GGYKQFL-NANGLKGKRLGIVRNPFLSFFNDSAITQAFEE 323
            G D ND  T   +   P  G Y +FL N   L+G R G+   P+   +  +A     + 
Sbjct: 302 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLW-QNAKADEIDR 361

Query: 324 HFNILKQ---GGAILVDNLEIANIDII--------LNVTASGEAVALLAEFKQSLNEYLK 383
              ++KQ    GAI+ +N    N+D+I        L      E   +  +F  ++  YL 
Sbjct: 362 LLEVVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLS 421

Query: 384 ELVASPVRSLADIIAFDNANPDQE-----LLDVF--GQEIFLAAEATNGIGDVQKAALLN 443
           E+  + + SL DI+ ++N     E     ++  F  GQ+ FLA+    G+ +      + 
Sbjct: 422 EVKNTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVE 481

Query: 444 LAKLTK--DGFE--------KVVEEERLDAVVTPGPAIATV--LAIGGFPGINVPAGYDG 479
             + T   +G +        K  +   L+ ++ P     T    A  G+P I +P G   
Sbjct: 482 YVRRTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKT 541

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.7e-58
Identity = 162/491 (32.99%), Postives = 239/491 (48.68%), Query Frame = 0


Query: 36  LQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKSDREREAKKPGSLC 95
           LQ  +++  +    +V+ Y+  I  +N  V  V EINPDAL  A + D ER   K G L 
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNER---KMGKLR 153

Query: 96  G-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEW 155
           G LHG+P+++K++I T DK+++TAGS+A+ G+    DA V  +LR AG +I+GK+  S+W
Sbjct: 154 GPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 156 ADF------------------------------SGPSISVAANIAAVSIGTETDGSILCP 215
           A+F                              SG  ++    +A  ++GTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 216 ASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRT 275
           A  +++VG+KPTVGLTSR  V+P+S RQDT+GP+ R+V DA  +L  I G D ND  T  
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSA 333

Query: 276 SSKYIPYGGYKQFLNA---NGLKGKRLGIVRNPFLSFFNDSAITQAFEEHFNILKQGGAI 335
               IP+     ++ A   N LKGKR+G+ RN    F +   +   F +   ++K+ GAI
Sbjct: 334 ----IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAI 393

Query: 336 LVDNLEIANIDIILNVTASGEAVALLAEFKQSLNEYLKELVASP--VRSLADIIAFDNAN 395
           +V+N +  +           +   L A+   +L  + K+L  +P  +  L  +  F   +
Sbjct: 394 IVENTDFTSFAEFAQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH 453

Query: 396 PDQEL--LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPG 455
             +E    D    +I L     N           N+    + G    +   +LDA V P 
Sbjct: 454 RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPT 513

Query: 456 PAIATVLAIGGFPGINVPAGY---------------DGGGVPFGINFGGLKGSEPKLIEV 474
                + A+ G P I VP G                 G G+P GI F G   SE KLI +
Sbjct: 514 DLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGL 573

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 153/503 (30.42%), Postives = 231/503 (45.92%), Query Frame = 0


Query: 32  TIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPD-ALLQAYKSDREREAKK 91
           +I  L       + T+  +   ++  I+ L P V   + + PD AL QA K D   +  +
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVD--EKIAR 62

Query: 92  PGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKAS 151
             SL  L GIP+ LKD++ TK  + TT  S  L   + P ++ V ++LR  GA+I+GK +
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 152 LSEWA-----------------DFS--------GPSISVAANIAAVSIGTETDGSILCPA 211
           L E+A                 D S        G + +VAA    V++G++T GSI  PA
Sbjct: 123 LDEFAMGSSTENSGYHVTANPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGGSIRQPA 182

Query: 212 SFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTS 271
           SF  VVG+KPT GL SR G++  +   D IGP GRTV DA I+L  I G+D  D    ++
Sbjct: 183 SFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQD----ST 242

Query: 272 SKYIPYGGYKQFLNANGLKGKRLGIVRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDN 331
           S  +P   Y QFL  + LKG ++G+++  F     D  + +A  +    LK  GA  +  
Sbjct: 243 SLNLPIPDYSQFLKTS-LKGLKIGVIKETFGEGL-DQVVAEAVNQALAQLKALGA-TIKE 302

Query: 332 LEIANIDIIL---NVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQ 391
           +        L    + A  EA A LA +     + +K  +     SL D+     A    
Sbjct: 303 ISCPRFRYGLPTYYIIAPSEASANLARY-----DGVKYGIREDADSLIDMYTKTRAKG-- 362

Query: 392 ELLDVFGQE----IFLAAEATN-GIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTP- 451
                FG E    I L     + G  D        +  L K+ F++  +   +D +V+P 
Sbjct: 363 -----FGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAFQS--VDVLVSPT 422

Query: 452 --------GPAIATVLA------------IGGFPGINVPAGYDGGGVPFGINFGGLKGSE 479
                   G   A  L+            + G PG+++P G+DG G+P G+   G    E
Sbjct: 423 SPTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQLVGNVLRE 481

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot
Match: B8HY89 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=gatA PE=3 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.3e-34
Identity = 145/508 (28.54%), Postives = 226/508 (44.49%), Query Frame = 0


Query: 31  ATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPD-ALLQAYKSDREREAK 90
           A+I +L       + ++  + +  + +I++L P VH  + +  + AL QA + D++    
Sbjct: 2   ASIRELHQQLVSKERSAKEITQDALEKIQQLEPKVHAFLTLTAEQALAQAERVDQQIAT- 61

Query: 91  KPGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGK 150
             G+  G L GIP+ +KD++ TK  + TT GS  L G I P ++ V  RL  AGA+++GK
Sbjct: 62  --GTEIGLLAGIPIAIKDNLCTKG-IPTTCGSKILQGFIPPYESTVTSRLAAAGAVMVGK 121

Query: 151 ASLSEWA-------------------------DFSGPSISVAANIAAVSIGTETDGSILC 210
            +L E+A                            G + +VAA    +++G++T GSI  
Sbjct: 122 TNLDEFAMGSSTENSAYQLTANPWDLQRVPGGSSGGSAAAVAAGETLIALGSDTGGSIRQ 181

Query: 211 PASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATR 270
           PASF  VVG+KPT GL SR G++  +   D IGP    V DA ++L  I G D  D    
Sbjct: 182 PASFCGVVGLKPTYGLVSRYGLVAYASSLDQIGPFATNVEDAALLLGAIAGHDPQD---- 241

Query: 271 TSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFLSFFNDSAITQAFEEHFNILKQGGAILV 330
           ++S  +P   Y QFL  + LKGK++GI++  +     D  + Q   +    L++ GA   
Sbjct: 242 STSLNVPIPDYTQFLIPD-LKGKKIGIIQETYGEGL-DPQVEQVTHKAIQQLEELGA--- 301

Query: 331 DNLEIANIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNA----- 390
              E+  I                  F+  L  Y    + +P  + A++  +D       
Sbjct: 302 ---EVREIS--------------CPRFRYGLPTY---YIIAPSEASANLARYDGVKYGFR 361

Query: 391 NPDQELL---------DVFGQE----IFLAAEATN-GIGDVQKAALLNLAKLTKDGFEKV 450
           +PD E L         + FG E    I +   A + G  D        +  L K  FE  
Sbjct: 362 SPDPENLLSMYTRTRAEGFGPEVKRRIMIGTYALSAGYYDAYYLKAQKVRTLIKQDFEAA 421

Query: 451 VEEERLDAVVTPGPAIATVLAIG----------------------GFPGINVPAGYDGGG 471
            E+  +D +V P  A  T  A G                      G PG+++P G+D  G
Sbjct: 422 FEQ--VDVLVCP-TAPTTAFAAGAKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDQQG 473

[Close]

BLAST of Cp4.1LG15g05780 vs. NCBI nr
Match: XP_022953021.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 471/510 (92.35%), Postives = 475/510 (93.14%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTV RSAAVRGFSIREAT+PDLQLAFKRN+LTS GLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGAILVDNLEI NIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

[Close]

BLAST of Cp4.1LG15g05780 vs. NCBI nr
Match: XP_023554278.1 (probable amidase At4g34880 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 480/536 (89.55%), Postives = 480/536 (89.55%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR
Sbjct: 17  MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF
Sbjct: 317 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQ--------------------------EL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQ                          EL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQVTQYSIHCSSHSNRIHSESIRIMVVQEL 436

Query: 421 LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL 480
           LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL
Sbjct: 437 LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL 496

[Close]

BLAST of Cp4.1LG15g05780 vs. NCBI nr
Match: KAG7011753.1 (hypothetical protein SDJN02_26659, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 471/510 (92.35%), Postives = 475/510 (93.14%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTV RSAAVRGFSIREAT+PDLQLAFKRN+LTS GLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFD NDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDCNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

[Close]

BLAST of Cp4.1LG15g05780 vs. NCBI nr
Match: KAG6572101.1 (putative amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 890 bits (2299), Expect = 0.0
Identity = 470/510 (92.16%), Postives = 474/510 (92.94%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTV RSAAVRGFSIREAT+PDLQLAFKRN+LTS GLVEFYI EIRR
Sbjct: 71  MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIREIRR 130

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 131 LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 190

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 191 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 250

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 251 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 310

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFD NDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 311 RQDTIGPIGRTVTDAVIVLDTIVGFDCNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 370

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF
Sbjct: 371 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 430

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 431 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 490

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 491 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 550

[Close]

BLAST of Cp4.1LG15g05780 vs. NCBI nr
Match: XP_022968892.1 (putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 468/510 (91.76%), Postives = 473/510 (92.75%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MA SSWLLIIVAFCLTV RSAAVRGFSIREAT+PDL LAFKRN+LTS GLVEFYIGEIRR
Sbjct: 17  MACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND ATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGA+LVDNLEIANIDIILNVTASGEA ALLAEF
Sbjct: 317 VRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 436

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 437 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 496

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL
Match: A0A6J1GLV2 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 471/510 (92.35%), Postives = 475/510 (93.14%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTV RSAAVRGFSIREAT+PDLQLAFKRN+LTS GLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGAILVDNLEI NIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL
Match: A0A6J1HUS8 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1)

HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 468/510 (91.76%), Postives = 473/510 (92.75%), Query Frame = 0


Query: 1   MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 60
           MA SSWLLIIVAFCLTV RSAAVRGFSIREAT+PDL LAFKRN+LTS GLVEFYIGEIRR
Sbjct: 17  MACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF----------------------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADF                       
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                  SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND ATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGA+LVDNLEIANIDIILNVTASGEA ALLAEF
Sbjct: 317 VRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 436

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 437 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 496

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL
Match: A0A0A0K2I0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=4 SV=1)

HSP 1 Score: 817 bits (2110), Expect = 8.13e-296
Identity = 431/506 (85.18%), Postives = 448/506 (88.54%), Query Frame = 0


Query: 5   SWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPV 64
           S LLI+VAFC T   S  VRG SIREAT+ DLQLAFK+NQLTS  LV FYIGEIRRLNPV
Sbjct: 13  SLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDALLQAYK+DREREA KPGSLCGLHGIPVLLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADF--------------------------- 184
           GSIVPRDAGVVKRLR+AGAIILGKASLSEWADF                           
Sbjct: 133 GSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
              SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSL 364
           F SFFNDS ITQAFE+HFN LKQGGAIL+DNLEIA+IDIILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA+LNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAAVLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 480
           KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL
Match: A0A1S4DXK2 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4 SV=1)

HSP 1 Score: 815 bits (2104), Expect = 6.66e-295
Identity = 427/506 (84.39%), Postives = 447/506 (88.34%), Query Frame = 0


Query: 5   SWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPV 64
           S LLI+VAFCLT      VRG SIREAT+ DLQLAFK+NQLTS  LV FYIGEIRRLNPV
Sbjct: 13  SMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDAL+QAYK+DREREA KPGSLCGLHGIP+LLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADF--------------------------- 184
           GSIVPRDAG+VKRLR+AGAIILGKASLSEWADF                           
Sbjct: 133 GSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
              SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSL 364
           F SFFNDS ITQAF++HFN LKQGGAIL+DNLEIANIDIILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAALLNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 480
           KLT+DGFEK+V++ +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

[Close]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL
Match: A0A5A7U6F5 (Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00780 PE=4 SV=1)

HSP 1 Score: 813 bits (2101), Expect = 1.91e-294
Identity = 427/506 (84.39%), Postives = 447/506 (88.34%), Query Frame = 0


Query: 5   SWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPV 64
           S LLI+VAFCLT      VRG SIREAT+ DLQLAFK+NQLTS  LV FYIGEIRRLNPV
Sbjct: 13  SLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDAL+QAYK+DREREA KPGSLCGLHGIP+LLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADF--------------------------- 184
           GSIVPRDAG+VKRLR+AGAIILGKASLSEWADF                           
Sbjct: 133 GSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
              SGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSL 364
           F SFFNDS ITQAF++HFN LKQGGAIL+DNLEIANI+IILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAALLNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 480
           KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

[Close]

BLAST of Cp4.1LG15g05780 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 519.6 bits (1337), Expect = 2.6e-147
Identity = 281/482 (58.30%), Postives = 359/482 (74.48%), Query Frame = 0


Query: 3   YSSWLLIIV---AFCLTVQRSAAVR---GFSIREATIPDLQLAFKRNQLTSTGLVEFYIG 62
           +SS L++++   A  ++V  ++ +R    FSI+EATI D+++AF   +LTS  LVE Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 63  EIRRLNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNT 122
            I +LNP++H VIE NPDAL+QA  +DRER+ K    L  LHG+PVLLKDSI TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 123 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFSGPSISVAANIAAVSIGTE 182
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASLSEWA F   SI              
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSI-------------- 188

Query: 183 TDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFD 242
            DG      S NSVVGIKP+VGLTSRAGV+P+S RQD+IGPI RTV+DAV +LD IVG+D
Sbjct: 189 PDG--WSAPSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYD 248

Query: 243 YNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFLSFFNDSAITQAFEEHFNILK 302
             DEAT+T+S++IP GGYKQFL  +GLKGKRLGIV             +   + H   L+
Sbjct: 249 PLDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKH----------SSLLDHHIKTLR 308

Query: 303 QGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFD 362
           + GAI+++NL I NI++I+  T SGE +ALLAEFK SLN YLKELV SPVRSLAD+IA++
Sbjct: 309 REGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYN 368

Query: 363 NANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTP 422
               +QE +  +GQE+FL AEAT+G+G+ +K AL  + +L+++G EK++EE +LDA+VT 
Sbjct: 369 EEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTL 428

Query: 423 GPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPKLIEVAYAFEQATLIRKPP 479
           G  +++VLAIGG+PGINVPAGYD GGVP+GI+FGGL+ SEPKLIE+A+AFEQATLIRKPP
Sbjct: 429 GSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 464

[Close]

BLAST of Cp4.1LG15g05780 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-25
Identity = 136/527 (25.81%), Postives = 228/527 (43.26%), Query Frame = 0


Query: 16  TVQRSAAVRGFSIREATIPDLQLAFKRNQL-----TSTGLVEFYIGEIRRLNPVVHGVIE 75
           T+  +A+ +       + P  Q+   R  L     T+  + + Y+  IR   P +   + 
Sbjct: 24  TIVSAASSKTIDTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLH 83

Query: 76  INPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPR 135
           ++ + L  A + D  +   K   L  L G+ + +KD+I T+  + +TA S  L     P 
Sbjct: 84  VSENVLKDAQEID--QRIAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPF 143

Query: 136 DAGVVKRLRRAGAIILGKASLSEWA-----------------DFS--------GPSISVA 195
           DA  VK+++  G I++GK ++ E+                  D S        G + +VA
Sbjct: 144 DATAVKKIKELGGIVVGKTNMDEFGMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVA 203

Query: 196 ANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDA 255
           A    VS+G++T GS+  PASF  VVG+KPT G  SR G++  +   D IG  G TV DA
Sbjct: 204 ARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADA 263

Query: 256 VIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLN-----ANGLKGKRLGIVRNPFLSFFN 315
            ++L  I G+D  D    TSSK        QFL+     +  L G ++GI+R   L    
Sbjct: 264 GMLLHAISGYDRFDS---TSSKQDVPEFQSQFLSVDHFESKPLNGVKVGIIRET-LEDGV 323

Query: 316 DSAITQAFEEHFNILKQGGAILVDNLEIANIDIIL---NVTASGEAVALLAEFKQSLNEY 375
           DS +  A +E  + L+  G IL + + + +  + L    V AS E+ + L+ +      Y
Sbjct: 324 DSGVRSATQEAASHLEALGCILTE-VSLPSFSLGLPAYYVIASSESSSNLSRYDGV--RY 383

Query: 376 LKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLT 435
             +++A  +  L +    +    + ++  + G     A     G  D        +  L 
Sbjct: 384 GNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSA-----GYYDAYYKRAQQVRTLI 443

Query: 436 KDGFEKVVEEERLDAVVTP---------------------GPAIATVLAIGGFPGINVPA 481
           +  F+  +E+   D +++P                     G  +   + + G P + +P 
Sbjct: 444 RKDFKAALEQN--DILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPC 503

[Close]

BLAST of Cp4.1LG15g05780 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 102.8 bits (255), Expect = 7.7e-22
Identity = 73/236 (30.93%), Postives = 115/236 (48.73%), Query Frame = 0


Query: 32  TIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKSDRER-EAKK 91
           ++ +L    K  Q+TS  LV  Y+ +++R N V+  V+    +    AYK  +E  +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL---AYKQAKEADDLLS 263

Query: 92  PGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 151
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 152 SLSEWA-----------------DFS-----GPSISVAANIAAVSIGTETDGSILCPASF 211
                A                 +FS     GP+ S +A +   +IG+ET GS+  PA+ 
Sbjct: 324 VTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAAR 383

Query: 212 NSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATR 244
             +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D ++R
Sbjct: 384 CGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSR 435

[Close]

BLAST of Cp4.1LG15g05780 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 3.2e-20
Identity = 70/229 (30.57%), Postives = 111/229 (48.47%), Query Frame = 0


Query: 32  TIPDLQLAFKRNQLTSTGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKSDRER-EAKK 91
           ++ +L    K  Q+TS  LV  Y+ +++R N V+  V+    +    AYK  +E  +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL---AYKQAKEADDLLS 263

Query: 92  PGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGK- 151
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 152 --------------ASLSEWADFSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIK 211
                          + + W      + S A   A+ S G+ET GS+  PA+   +  ++
Sbjct: 324 VTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGSETAGSMTYPAARCGITALR 383

Query: 212 PTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATR 244
           PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D ++R
Sbjct: 384 PTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSR 428

[Close]

BLAST of Cp4.1LG15g05780 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 86.7 bits (213), Expect = 5.7e-17
Identity = 106/446 (23.77%), Postives = 184/446 (41.26%), Query Frame = 0


Query: 74  DALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAG 133
           + + QA  S R  E   P S+  L GI V +KD I           ++      V +D+ 
Sbjct: 176 EVIKQAEASTRRFEQGNPISV--LDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSA 235

Query: 134 VVKRLRRAGAIILGKASLSE-------------------------WADFSGPSISVAANI 193
           VV +LR  GAI+LGKA++ E                             SG +  VAA +
Sbjct: 236 VVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGL 295

Query: 194 AAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIV 253
            + ++GT+  GS+  P++   + G+K T G T   G +      + IGP+  ++ DA +V
Sbjct: 296 CSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLV 355

Query: 254 LDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVR-NPFLSFFNDSAITQA 313
              I+G   +  A R + K  P   + + L+ NG     +G +R   +  +FND + +  
Sbjct: 356 YAAILG---SSSADRYNLKPSP-PCFPKLLSHNG--SNAIGSLRLGKYTKWFNDVSSSDI 415

Query: 314 FEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVR 373
            ++  +ILK    +L +N     ++I++       A  +++    +L+       A    
Sbjct: 416 SDKCEDILK----LLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNS 475

Query: 374 SLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEE 433
            L+      +      +   F    ++AA+       +++  +     + KD    V   
Sbjct: 476 KLS-----YDTRTSFAIFRSFSASDYIAAQC------LRRRLMEYHLNIFKDVDVIVTPT 535

Query: 434 ERLDAVVTPGPAIAT--------------VLAIG--GFPGINVPAGYDGGGVPFGINFGG 478
             + A V P  A+                VLA    GFP I+VP GYD  G+P G+   G
Sbjct: 536 TGMTAPVIPPDALKNGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMG 595

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g05780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8B760	3.0e-156	58.20	Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=...	[more]
Q9URY4	6.9e-68	35.91	Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
D4B3C8	1.7e-58	32.99	Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ...	[more]
B0JSX3	1.5e-38	30.42	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ...	[more]
B8HY89	1.3e-34	28.54	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cyanothece sp. (strain PCC 7425...	[more]

BLAST of Cp4.1LG15g05780 vs. NCBI nr


Match Name	Identity	Description
XP_022953021.1	92.35	putative amidase C869.01 [Cucurbita moschata]	[more]
XP_023554278.1	89.55	probable amidase At4g34880 [Cucurbita pepo subsp. pepo]	[more]
KAG7011753.1	92.35	hypothetical protein SDJN02_26659, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6572101.1	92.16	putative amidase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022968892.1	91.76	putative amidase C869.01 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG15g05780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GLV2	0.0	92.35	putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1	[more]
A0A6J1HUS8	0.0	91.76	putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1	[more]
A0A0A0K2I0	8.13e-296	85.18	Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=...	[more]
A0A1S4DXK2	6.66e-295	84.39	putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4...	[more]
A0A5A7U6F5	1.91e-294	84.39	Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]

BLAST of Cp4.1LG15g05780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34880.1	2.6e-147	58.30	Amidase family protein	[more]
AT3G25660.1	2.0e-25	25.81	Amidase family protein	[more]
AT5G07360.1	7.7e-22	30.93	Amidase family protein	[more]
AT5G07360.2	3.2e-20	30.57	Amidase family protein	[more]
AT5G64440.1	5.7e-17	23.77	fatty acid amide hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 158..418 e-value: 1.8E-33 score: 116.3 coord: 50..157 e-value: 9.1E-22 score: 77.7
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 14..478 e-value: 8.2E-124 score: 416.3
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 27..477
None	No IPR available	PANTHER	PTHR42678:SF19	C869.01-LIKE PROTEIN, PUTATIVE-RELATED	coord: 21..157 coord: 158..480
None	No IPR available	PANTHER	PTHR42678	AMIDASE	coord: 21..157 coord: 158..480

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g05780.1	Cp4.1LG15g05780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane