Cp4.1LG15g05440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG15g05440 vs. ExPASy Swiss-Prot
Match: O81355 (Phenylcoumaran benzylic ether reductase Pyrc5 OS=Pyrus communis OX=23211 GN=PYRC5 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 4.6e-104
Identity = 190/304 (62.50%), Postives = 230/304 (75.66%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +Q+L +G TGYIGKFIVEAS K G+ T+ LVR+++LSDPAKS +I+ FK++GV  V GDL
Sbjct: 5   SQILFIGGTGYIGKFIVEASAKAGYPTYVLVREASLSDPAKSKVIENFKALGVNFVLGDL 64

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
           +DHESLVKAIKQVDVVIST                       RFFPSEFG DV+  H V 
Sbjct: 65  YDHESLVKAIKQVDVVISTVGHGQLADQGKIIAAIKEAGNVKRFFPSEFGNDVDRSHAVE 124

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGNP 523
           P KS FE+K +IRRAVEAEGIPYTYV S  F G+ +P+L Q G ++ PRDKVVI GDGNP
Sbjct: 125 PAKSAFETKAKIRRAVEAEGIPYTYVSSNFFAGYFLPTLNQPGASSAPRDKVVILGDGNP 184

Query: 524 KAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVS 583
           KAIFN E+DI ++TI+AVDDP TLNK+LYI+P  N +S N+LV+LWE KIG  L + +V 
Sbjct: 185 KAIFNKEDDIGTYTIRAVDDPRTLNKVLYIRPPANTISFNELVSLWEKKIGKTLERIYVP 244

Query: 584 EHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYL 625
           E QLLKNI EAA PLN++L+  +++FVKGDHTN EIE S G+EAT+LYPDVKY TVDEYL
Sbjct: 245 EEQLLKNIQEAAVPLNVILSISHAVFVKGDHTNFEIEPSFGVEATALYPDVKYTTVDEYL 304

BLAST of Cp4.1LG15g05440 vs. ExPASy Swiss-Prot
Match: B2WSN0 (Eugenol synthase 2 OS=Clarkia breweri OX=36903 GN=EGS2 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 7.8e-104
Identity = 187/305 (61.31%), Postives = 231/305 (75.74%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++L++G TGYIGKFIVEASVK GH TFALVR++ +SDP K  +++KF+++GV ++ GDL
Sbjct: 5   SKILIIGGTGYIGKFIVEASVKEGHPTFALVRETTVSDPVKGKLVEKFQNLGVSLLYGDL 64

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
           +DH+SLVKAIKQVDVVIST                       RFFPSEFG DV++V+ V 
Sbjct: 65  YDHDSLVKAIKQVDVVISTVGFMQIADQTKIIAAIKEAGNVKRFFPSEFGNDVDHVNAVE 124

Query: 464 PVKSI-FESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGN 523
           P KS+ F  K  IRRAVEAEGIPYTYV S CFNG+ +P+L Q G T PPRDKV+I GDGN
Sbjct: 125 PAKSVAFAVKANIRRAVEAEGIPYTYVASNCFNGYFLPTLVQPGATTPPRDKVIIPGDGN 184

Query: 524 PKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFV 583
           PKAIFN EEDI ++TIKAVDDP TLNKILY++PS N+ S N+LVALWE KIG  L K +V
Sbjct: 185 PKAIFNKEEDIGTYTIKAVDDPRTLNKILYLRPSNNIYSFNELVALWEKKIGKTLEKIYV 244

Query: 584 SEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEY 625
            E Q+LK+I EA  P+N+ L   +S+FVKGDHTN EIE S G+EA+ LYP+VKY TV+EY
Sbjct: 245 PEEQILKDIQEAPIPINIFLGINHSVFVKGDHTNFEIEPSFGVEASELYPEVKYTTVEEY 304

BLAST of Cp4.1LG15g05440 vs. ExPASy Swiss-Prot
Match: B9HRL7 (Phenylcoumaran benzylic ether reductase POP1 OS=Populus trichocarpa OX=3694 GN=PCBER PE=1 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.5e-102
Identity = 190/304 (62.50%), Postives = 225/304 (74.01%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++L +G TGYIGKFIVEAS K GH TF LVR+S LS+PAKS +I  FK++GV  + GDL
Sbjct: 3   SKILFIGGTGYIGKFIVEASAKAGHPTFVLVRESTLSNPAKSVVIYNFKNLGVNFLIGDL 62

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
           FDHESLVKAIKQVDVVIST                       RFFPSEFG DV+ V+ V 
Sbjct: 63  FDHESLVKAIKQVDVVISTVGHAQLVEQDRIIAAIKEAGNVKRFFPSEFGNDVDRVNAVE 122

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGNP 523
           P KS F +K  +RRA+EAEGIPYTYV S  F+G+ + S  Q G TAPPRDKVVI GDGNP
Sbjct: 123 PAKSAFATKANVRRAIEAEGIPYTYVSSNFFSGYFLLSFNQPGATAPPRDKVVILGDGNP 182

Query: 524 KAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVS 583
           KA+FN E+DIA++TIKAVDDP TLNKILYIKP  N +S N LV+LWE KIG  L + +V 
Sbjct: 183 KAVFNKEDDIATYTIKAVDDPRTLNKILYIKPPANTISFNDLVSLWEKKIGKTLERIYVP 242

Query: 584 EHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYL 625
           E QLLKNI EA+ P+N++L+  +S+FVKGDHTN EIE S G+EA+ LYPDVKY TVDEYL
Sbjct: 243 EEQLLKNIQEASVPVNVVLSIGHSVFVKGDHTNFEIEPSFGVEASELYPDVKYTTVDEYL 302

BLAST of Cp4.1LG15g05440 vs. ExPASy Swiss-Prot
Match: Q9FUW6 (Phenylcoumaran benzylic ether reductase Betv6 OS=Betula pendula OX=3505 GN=BETV6 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.6e-101
Identity = 180/304 (59.21%), Postives = 228/304 (75.00%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++L++G TGYIGKFIVEAS K GH TFALVR+S +SDP K  +++KFK +GV ++ GDL
Sbjct: 5   SKILIIGGTGYIGKFIVEASAKSGHPTFALVRESTVSDPVKGKLVEKFKGLGVTLLHGDL 64

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
           +DHESLVKA KQVDVVIST                       RFFPSEFG DV+ VH V 
Sbjct: 65  YDHESLVKAFKQVDVVISTVGHLQLADQVKIIAAIKEAGNIKRFFPSEFGNDVDRVHAVE 124

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGNP 523
           P K+ F +K +IRR  EAEGIPYTYV S  F G+ +P+L Q G+T+PPR+KVVI GDGN 
Sbjct: 125 PAKTAFATKAEIRRKTEAEGIPYTYVSSNFFAGYFLPTLAQPGLTSPPREKVVIFGDGNA 184

Query: 524 KAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVS 583
           +A+FN E+DI ++TI+AVDDP TLNKI+YIKP++N+ S N++VALWE KIG  L K +V 
Sbjct: 185 RAVFNKEDDIGTYTIRAVDDPRTLNKIVYIKPAKNIYSFNEIVALWEKKIGKTLEKIYVP 244

Query: 584 EHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYL 625
           E +LLK+I E+  P+N++LA  +S+FVKGDHTN EIEAS G+EA+ LYPDVKY TV+EYL
Sbjct: 245 EEKLLKDIQESPIPINVILAINHSVFVKGDHTNFEIEASFGVEASELYPDVKYTTVEEYL 304

BLAST of Cp4.1LG15g05440 vs. ExPASy Swiss-Prot
Match: B6VRE8 (Phenylcoumaran benzylic ether reductase TP7 OS=Nicotiana tabacum OX=4097 GN=TP7 PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 6.4e-98
Identity = 178/304 (58.55%), Postives = 219/304 (72.04%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           ++VL++G TGYIGKF+VEAS K GH TFALVR+S LSDP KS I++ FK++GV ++ GDL
Sbjct: 5   SKVLIIGGTGYIGKFVVEASAKSGHPTFALVRESTLSDPVKSKIVENFKNLGVTILHGDL 64

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
           +DHESLVKAIKQVDVVIST                       RFFPSEFG DV+  + V 
Sbjct: 65  YDHESLVKAIKQVDVVISTMGMMQLGDQVKLIAAIKEAGNIKRFFPSEFGMDVDKTNAVE 124

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGNP 523
           P KS F  KVQIRRA+EAEGIPYTYV   CF G+ +P++ Q G T PPRDKV+I GDGN 
Sbjct: 125 PAKSAFAVKVQIRRAIEAEGIPYTYVSCNCFAGYFLPTMVQPGATVPPRDKVIIPGDGNV 184

Query: 524 KAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVS 583
           KA+FN E DI ++TIKAVDDP TLNK LYIKP +N LS N+LVA+WE  IG  L K ++ 
Sbjct: 185 KAVFNEEHDIGTYTIKAVDDPRTLNKTLYIKPPKNTLSFNELVAMWEKMIGKTLEKIYIP 244

Query: 584 EHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYL 625
           E Q+LK+I  +  PL ++LA  ++ FVKGD TN +IE S G+EA+ LYPDVKY TV++YL
Sbjct: 245 EEQILKDIETSPMPLPVILAINHATFVKGDQTNFKIEPSFGVEASELYPDVKYTTVEDYL 304

BLAST of Cp4.1LG15g05440 vs. NCBI nr
Match: XP_023553613.1 (isoflavone reductase homolog PCBER-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746 bits (1926), Expect = 3.53e-264
Identity = 421/649 (64.87%), Postives = 465/649 (71.65%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIK-------------- 120
           SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIK              
Sbjct: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKRFFPSEYGNDVDRV 120

Query: 121 ---VRTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVV 180
                 RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVV
Sbjct: 121 HGVEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVV 180

Query: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKI 240
           ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKI
Sbjct: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKI 240

Query: 241 LDKSYGDQTNF--DIE---PSFGVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAKAS 300
           LDKSY  +     DI+   P+  ++A      +       F+  TGHVGKFVVEASAKAS
Sbjct: 241 LDKSYVSEQQLLNDIQASWPNIWIKAMASKSKIL------FIGSTGHVGKFVVEASAKAS 300

Query: 301 HPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEYTT 360
           HPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                   +
Sbjct: 301 HPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE-------------------S 360

Query: 361 VDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIV 420
           +   + QV VV ST              GH   A                          
Sbjct: 361 LVMAIKQVDVVIST-------------VGHNLLA-------------------------- 420

Query: 421 VSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAVEA 480
                 D + ++ AIK+   V   RFFPSE+G DV++VH V P ++ F +K  IRRAVEA
Sbjct: 421 ------DQDRIISAIKETGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAVEA 480

Query: 481 EGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASFTI 540
             IPYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E+D+A++ I
Sbjct: 481 AQIPYTFVSSNLFSGWFLPSLAQPYLLEVVTPPEDKVTILGDG--KAIFNVEQDVATYII 540

Query: 541 KAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAYPL 600
           KAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++PL
Sbjct: 541 KAVDDPRTLNKILYVRPLQNTYSCVDLVALWEKKCGKILDKSYVSEQQLLNDIREASFPL 575

Query: 601 NLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           NL L+  YS+FVKGD TN +IE S G+EAT LYPD++Y TVDE+LNQFV
Sbjct: 601 NLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDIEYTTVDEFLNQFV 575

BLAST of Cp4.1LG15g05440 vs. NCBI nr
Match: XP_023553612.1 (isoflavone reductase homolog PCBER-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746 bits (1925), Expect = 5.78e-264
Identity = 421/653 (64.47%), Postives = 466/653 (71.36%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKV------------- 120
           SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIK+             
Sbjct: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKLIVQRFFPSEYGND 120

Query: 121 --------RTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180
                     RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK
Sbjct: 121 VDRVHGVEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180

Query: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240
           DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK
Sbjct: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240

Query: 241 CGKILDKSYGDQTNF--DIE---PSFGVEATELYPDVKYTTVDEFLNHTGHVGKFVVEAS 300
           CGKILDKSY  +     DI+   P+  ++A      +       F+  TGHVGKFVVEAS
Sbjct: 241 CGKILDKSYVSEQQLLNDIQASWPNIWIKAMASKSKIL------FIGSTGHVGKFVVEAS 300

Query: 301 AKASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDI 360
           AKASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                
Sbjct: 301 AKASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE---------------- 360

Query: 361 EYTTVDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSI 420
              ++   + QV VV ST              GH   A                      
Sbjct: 361 ---SLVMAIKQVDVVIST-------------VGHNLLA---------------------- 420

Query: 421 GVIVVSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRR 480
                     D + ++ AIK+   V   RFFPSE+G DV++VH V P ++ F +K  IRR
Sbjct: 421 ----------DQDRIISAIKETGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRR 480

Query: 481 AVEAEGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIA 540
           AVEA  IPYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E+D+A
Sbjct: 481 AVEAAQIPYTFVSSNLFSGWFLPSLAQPYLLEVVTPPEDKVTILGDG--KAIFNVEQDVA 540

Query: 541 SFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEA 600
           ++ IKAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA
Sbjct: 541 TYIIKAVDDPRTLNKILYVRPLQNTYSCVDLVALWEKKCGKILDKSYVSEQQLLNDIREA 579

Query: 601 AYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           ++PLNL L+  YS+FVKGD TN +IE S G+EAT LYPD++Y TVDE+LNQFV
Sbjct: 601 SFPLNLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDIEYTTVDEFLNQFV 579

BLAST of Cp4.1LG15g05440 vs. NCBI nr
Match: XP_022953095.1 (isoflavone reductase-like protein isoform X2 [Cucurbita moschata])

HSP 1 Score: 719 bits (1857), Expect = 1.06e-253
Identity = 410/647 (63.37%), Postives = 455/647 (70.32%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIK-------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K              
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKRFFPSEYGNDVDRV 120

Query: 121 ---VRTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVV 180
                 RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPKDKVV
Sbjct: 121 HGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPKDKVV 180

Query: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKI 240
           ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK GKI
Sbjct: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKYGKI 240

Query: 241 LDKSYGDQTNF--DIEPSF-GVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAKASHP 300
           LDKSY  +     DI+ S+  +   E+    K      F+  TGHVGK VVEASAKASHP
Sbjct: 241 LDKSYVSEQQLLNDIQASWPNIWIKEMASKSKIL----FIGSTGHVGKHVVEASAKASHP 300

Query: 301 TYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEYTTVD 360
           TYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                   ++ 
Sbjct: 301 TYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE-------------------SLV 360

Query: 361 EFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVS 420
             + QV VV ST              GH   A                            
Sbjct: 361 MAIKQVDVVIST-------------VGHNLLA---------------------------- 420

Query: 421 GDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAVEAEG 480
               D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAVEA  
Sbjct: 421 ----DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAVEAAQ 480

Query: 481 IPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASFTIKA 540
           +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++ IKA
Sbjct: 481 VPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATYIIKA 540

Query: 541 VDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAYPLNL 600
           VDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++PLNL
Sbjct: 541 VDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASFPLNL 575

Query: 601 MLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
            L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 RLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 575

BLAST of Cp4.1LG15g05440 vs. NCBI nr
Match: XP_022953094.1 (isoflavone reductase-like protein isoform X1 [Cucurbita moschata])

HSP 1 Score: 719 bits (1856), Expect = 1.73e-253
Identity = 410/651 (62.98%), Postives = 456/651 (70.05%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKV------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K+             
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKLIVQRFFPSEYGND 120

Query: 121 --------RTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180
                     RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPK
Sbjct: 121 VDRVHGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPK 180

Query: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240
           DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK
Sbjct: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240

Query: 241 CGKILDKSYGDQTNF--DIEPSF-GVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAK 300
            GKILDKSY  +     DI+ S+  +   E+    K      F+  TGHVGK VVEASAK
Sbjct: 241 YGKILDKSYVSEQQLLNDIQASWPNIWIKEMASKSKIL----FIGSTGHVGKHVVEASAK 300

Query: 301 ASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEY 360
           ASHPTYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                  
Sbjct: 301 ASHPTYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE------------------ 360

Query: 361 TTVDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGV 420
            ++   + QV VV ST              GH   A                        
Sbjct: 361 -SLVMAIKQVDVVIST-------------VGHNLLA------------------------ 420

Query: 421 IVVSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAV 480
                   D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAV
Sbjct: 421 --------DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAV 480

Query: 481 EAEGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASF 540
           EA  +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++
Sbjct: 481 EAAQVPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATY 540

Query: 541 TIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAY 600
            IKAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++
Sbjct: 541 IIKAVDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASF 579

Query: 601 PLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           PLNL L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 PLNLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 579

BLAST of Cp4.1LG15g05440 vs. NCBI nr
Match: XP_022953096.1 (isoflavone reductase-like protein isoform X3 [Cucurbita moschata])

HSP 1 Score: 717 bits (1851), Expect = 7.19e-253
Identity = 408/651 (62.67%), Postives = 452/651 (69.43%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKV------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K+             
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKLIVQRFFPSEYGND 120

Query: 121 --------RTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180
                     RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPK
Sbjct: 121 VDRVHGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPK 180

Query: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240
           DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK
Sbjct: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240

Query: 241 CGKILDKSYGDQTNFDIEPSFGVEATELYPDVKYTTVDE---FLNHTGHVGKFVVEASAK 300
            GKILDKSY             V   +L  D++         F+  TGHVGK VVEASAK
Sbjct: 241 YGKILDKSY-------------VSEQQLLNDIQEMASKSKILFIGSTGHVGKHVVEASAK 300

Query: 301 ASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEY 360
           ASHPTYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                  
Sbjct: 301 ASHPTYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE------------------ 360

Query: 361 TTVDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGV 420
            ++   + QV VV ST              GH   A                        
Sbjct: 361 -SLVMAIKQVDVVIST-------------VGHNLLA------------------------ 420

Query: 421 IVVSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAV 480
                   D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAV
Sbjct: 421 --------DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAV 480

Query: 481 EAEGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASF 540
           EA  +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++
Sbjct: 481 EAAQVPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATY 540

Query: 541 TIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAY 600
            IKAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++
Sbjct: 541 IIKAVDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASF 570

Query: 601 PLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           PLNL L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 PLNLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 570

BLAST of Cp4.1LG15g05440 vs. ExPASy TrEMBL
Match: A0A6J1GNQ2 (isoflavone reductase-like protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455600 PE=4 SV=1)

HSP 1 Score: 719 bits (1857), Expect = 5.12e-254
Identity = 410/647 (63.37%), Postives = 455/647 (70.32%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIK-------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K              
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKRFFPSEYGNDVDRV 120

Query: 121 ---VRTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVV 180
                 RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPKDKVV
Sbjct: 121 HGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPKDKVV 180

Query: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKI 240
           ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK GKI
Sbjct: 181 ILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKYGKI 240

Query: 241 LDKSYGDQTNF--DIEPSF-GVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAKASHP 300
           LDKSY  +     DI+ S+  +   E+    K      F+  TGHVGK VVEASAKASHP
Sbjct: 241 LDKSYVSEQQLLNDIQASWPNIWIKEMASKSKIL----FIGSTGHVGKHVVEASAKASHP 300

Query: 301 TYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEYTTVD 360
           TYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                   ++ 
Sbjct: 301 TYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE-------------------SLV 360

Query: 361 EFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVS 420
             + QV VV ST              GH   A                            
Sbjct: 361 MAIKQVDVVIST-------------VGHNLLA---------------------------- 420

Query: 421 GDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAVEAEG 480
               D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAVEA  
Sbjct: 421 ----DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAVEAAQ 480

Query: 481 IPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASFTIKA 540
           +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++ IKA
Sbjct: 481 VPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATYIIKA 540

Query: 541 VDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAYPLNL 600
           VDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++PLNL
Sbjct: 541 VDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASFPLNL 575

Query: 601 MLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
            L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 RLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 575

BLAST of Cp4.1LG15g05440 vs. ExPASy TrEMBL
Match: A0A6J1GNN6 (isoflavone reductase-like protein isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455600 PE=4 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 8.39e-254
Identity = 410/651 (62.98%), Postives = 456/651 (70.05%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKV------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K+             
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKLIVQRFFPSEYGND 120

Query: 121 --------RTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180
                     RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPK
Sbjct: 121 VDRVHGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPK 180

Query: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240
           DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK
Sbjct: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240

Query: 241 CGKILDKSYGDQTNF--DIEPSF-GVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAK 300
            GKILDKSY  +     DI+ S+  +   E+    K      F+  TGHVGK VVEASAK
Sbjct: 241 YGKILDKSYVSEQQLLNDIQASWPNIWIKEMASKSKIL----FIGSTGHVGKHVVEASAK 300

Query: 301 ASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEY 360
           ASHPTYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                  
Sbjct: 301 ASHPTYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE------------------ 360

Query: 361 TTVDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGV 420
            ++   + QV VV ST              GH   A                        
Sbjct: 361 -SLVMAIKQVDVVIST-------------VGHNLLA------------------------ 420

Query: 421 IVVSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAV 480
                   D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAV
Sbjct: 421 --------DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAV 480

Query: 481 EAEGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASF 540
           EA  +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++
Sbjct: 481 EAAQVPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATY 540

Query: 541 TIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAY 600
            IKAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++
Sbjct: 541 IIKAVDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASF 579

Query: 601 PLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           PLNL L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 PLNLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 579

BLAST of Cp4.1LG15g05440 vs. ExPASy TrEMBL
Match: A0A6J1GM17 (isoflavone reductase-like protein isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455600 PE=4 SV=1)

HSP 1 Score: 717 bits (1851), Expect = 3.48e-253
Identity = 408/651 (62.67%), Postives = 452/651 (69.43%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKV------------- 120
           SGDLF HESLVK IKQVDVVISTIGHSLL DQDRIISAIKEAGN+K+             
Sbjct: 61  SGDLFVHESLVKAIKQVDVVISTIGHSLLPDQDRIISAIKEAGNVKLIVQRFFPSEYGND 120

Query: 121 --------RTRTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPK 180
                     RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPK
Sbjct: 121 VDRVHGIEPARTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPK 180

Query: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240
           DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK
Sbjct: 181 DKVVILGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK 240

Query: 241 CGKILDKSYGDQTNFDIEPSFGVEATELYPDVKYTTVDE---FLNHTGHVGKFVVEASAK 300
            GKILDKSY             V   +L  D++         F+  TGHVGK VVEASAK
Sbjct: 241 YGKILDKSY-------------VSEQQLLNDIQEMASKSKILFIGSTGHVGKHVVEASAK 300

Query: 301 ASHPTYALLSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEY 360
           ASHPTYAL+SDSTLSDPTKSHIIDHFKSLKVIFISGD  N +                  
Sbjct: 301 ASHPTYALVSDSTLSDPTKSHIIDHFKSLKVIFISGDLYNHE------------------ 360

Query: 361 TTVDEFLNQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGV 420
            ++   + QV VV ST              GH   A                        
Sbjct: 361 -SLVMAIKQVDVVIST-------------VGHNLLA------------------------ 420

Query: 421 IVVSGDLFDHESLVKAIKQVDVVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAV 480
                   D   ++ AIK+V  V   RFFPSE+G DV++VH V P ++ F +K  IRRAV
Sbjct: 421 --------DQNRIISAIKEVGNV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAV 480

Query: 481 EAEGIPYTYVLSYCFNGFCVPSLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASF 540
           EA  +PYT+V S  F+G+ +PSL Q   L V  PP DKV I GDG  KAIFN E D+A++
Sbjct: 481 EAAQVPYTFVSSNLFSGWFLPSLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATY 540

Query: 541 TIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAY 600
            IKAVDDP TLNKILY++P +N  S   LVALWE K G IL K++VSE QLL +I EA++
Sbjct: 541 IIKAVDDPRTLNKILYVRPPQNTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASF 570

Query: 601 PLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           PLNL L+  YS+FVKGD TN +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 PLNLRLSIFYSVFVKGDQTNFDIEPSFGVEATKLYPDVEYTTVDEFLNQFV 570

BLAST of Cp4.1LG15g05440 vs. ExPASy TrEMBL
Match: A0A371FBZ4 (Uncharacterized protein (Fragment) OS=Mucuna pruriens OX=157652 GN=CR513_44265 PE=4 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 5.96e-228
Identity = 363/675 (53.78%), Postives = 453/675 (67.11%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MA KSKILIIGGTGYIGK IVEASA AG+PT  LVR ST+SDPAK +LI++F  LGV  V
Sbjct: 23  MAEKSKILIIGGTGYIGKHIVEASAKAGHPTFALVRESTISDPAKAQLIHNFKALGVNLV 82

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEA----------GNIKVRT- 120
            GDL++HE LV  IKQVDVVIST+GH  LADQ +II+AIKEA          GN   R  
Sbjct: 83  HGDLYDHEKLVTAIKQVDVVISTVGHLQLADQVKIIAAIKEADSTRFFPSEFGNDVDRVH 142

Query: 121 -----RTAFNAKAGIRRAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVVI 180
                ++AF  K  IRRA+EA  IPYTYVSSN  +G+FLP+LAQ   + A  PPKDKV+I
Sbjct: 143 AVEPAKSAFAIKVQIRRAIEAEGIPYTYVSSNCFAGYFLPTLAQPGAI-APPPPKDKVII 202

Query: 181 LGDGNFKAIFNVERDIATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKIL 240
           LGDGN KAIFN E DI TY I+AVDDPRTLNKILY+RP +N YS+ +LVALWE+K GK L
Sbjct: 203 LGDGNPKAIFNKEEDIGTYTIRAVDDPRTLNKILYLRPPKNIYSFNELVALWEKKIGKTL 262

Query: 241 DKSYGDQTNFDIEPSFGVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAKASHPTYAL 300
           +K Y             V   ++  D++                       +A  P   +
Sbjct: 263 EKIY-------------VPEEKVLKDIE-----------------------EAPLPINVI 322

Query: 301 LSDSTLSDPTKSHIIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEYTTVDEFLN 360
           L+            I+H      +F+ GD TNF+IE SFGVEA++LYPD++YTTV+E+L 
Sbjct: 323 LA------------INHS-----VFVKGDHTNFEIESSFGVEASELYPDVKYTTVEEYLG 382

Query: 361 QV----------LVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSI 420
           QV          L++G TGYIGK IVEAS K  H TFALVR+  +SDP K+ ++ KFK++
Sbjct: 383 QVVASMAGKSKILIIGGTGYIGKHIVEASAKAEHPTFALVRERTISDPEKATLLDKFKTL 442

Query: 421 GVIVVSGDLFDHESLVKAIKQVDVVIST-----------------------RFFPSEFGY 480
           GV +V GD++DHE LVKA+KQVDVVIS                        RFFPS+FG 
Sbjct: 443 GVNLVPGDMYDHEKLVKAMKQVDVVISAVGHLQLADQVNIISAIKEAGNIKRFFPSDFGN 502

Query: 481 DVENVHGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPP--R 540
           DV+ VH V P KS+F  K QIRR +EAEGIPYTYV + CF G+ +P+  Q G TAPP  +
Sbjct: 503 DVDRVHAVEPAKSLFAVKAQIRRTIEAEGIPYTYVSTNCFAGYFLPTFAQPGATAPPPPK 562

Query: 541 DKVVIHGDGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENK 600
            KV+I GDGNPKAIFNNEEDI ++TI+AVDDP TLNK+LY++P +N+ S N++V+LWE K
Sbjct: 563 HKVIIIGDGNPKAIFNNEEDIGTYTIRAVDDPRTLNKVLYLRPPKNIFSFNEVVSLWEKK 622

Query: 601 IGIILHKTFVSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYP 624
           IG  LHKT+V   +LLK+I EA  P+ + LA  +S+FVKGDH+N EIE+S G+EA+ LYP
Sbjct: 623 IGNTLHKTYVPGEKLLKDIEEAPVPIKMGLAISHSVFVKGDHSNFEIESSFGVEASELYP 643

BLAST of Cp4.1LG15g05440 vs. ExPASy TrEMBL
Match: A0A6J1GM98 (isoflavone reductase-like protein isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111455600 PE=4 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 7.44e-223
Identity = 377/630 (59.84%), Postives = 424/630 (67.30%), Query Frame = 0

Query: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGNPTHVLVRGSTLSDPAKFELINHFNTLGVMFV 60
           MAAKSKILIIGGTGYIGKFIVEASAMAG+PTHVLVRGSTLSDPAK ELINHFNTLGV+FV
Sbjct: 1   MAAKSKILIIGGTGYIGKFIVEASAMAGHPTHVLVRGSTLSDPAKSELINHFNTLGVIFV 60

Query: 61  SGDLFNHESLVKTIKQVDVVISTIGHSLLADQDRIISAIKEAGNIKVRTRTAFNAKAGIR 120
           S   F  E                G+    D DR+   I+ A       RTAFNAKAGIR
Sbjct: 61  SR-FFPSEY---------------GN----DVDRV-HGIEPA-------RTAFNAKAGIR 120

Query: 121 RAVEAAQIPYTYVSSNFCSGWFLPSLAQSYLLEATTPPKDKVVILGDGNFKAIFNVERDI 180
           RAVEAAQIPYTYVSSNFCSGWFLPSLAQ YLLEA  PPKDKVVILGDGNFKAIFNVERDI
Sbjct: 121 RAVEAAQIPYTYVSSNFCSGWFLPSLAQPYLLEAAAPPKDKVVILGDGNFKAIFNVERDI 180

Query: 181 ATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKCGKILDKSYGDQTNF--DIEP 240
           ATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQK GKILDKSY  +     DI+ 
Sbjct: 181 ATYIIKAVDDPRTLNKILYVRPLQNTYSYVDLVALWEQKYGKILDKSYVSEQQLLNDIQA 240

Query: 241 SF-GVEATELYPDVKYTTVDEFLNHTGHVGKFVVEASAKASHPTYALLSDSTLSDPTKSH 300
           S+  +   E+    K      F+  TGHVGK VVEASAKASHPTYAL+SDSTLSDPTKSH
Sbjct: 241 SWPNIWIKEMASKSKIL----FIGSTGHVGKHVVEASAKASHPTYALVSDSTLSDPTKSH 300

Query: 301 IIDHFKSLKVIFISGDQTNFDIEPSFGVEATKLYPDIEYTTVDEFLNQVLVVGSTGYIGK 360
           IIDHFKSLKVIFISGD  N +                   ++   + QV VV ST     
Sbjct: 301 IIDHFKSLKVIFISGDLYNHE-------------------SLVMAIKQVDVVIST----- 360

Query: 361 FIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDLFDHESLVKAIKQVD 420
                    GH   A                                D   ++ AIK+V 
Sbjct: 361 --------VGHNLLA--------------------------------DQNRIISAIKEVG 420

Query: 421 VVISTRFFPSEFGYDVENVHGVGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVP 480
            V   RFFPSE+G DV++VH V P ++ F +K  IRRAVEA  +PYT+V S  F+G+ +P
Sbjct: 421 NV--KRFFPSEYGCDVDHVHDVEPARTAFNAKAGIRRAVEAAQVPYTFVSSNLFSGWFLP 480

Query: 481 SLGQ---LGVTAPPRDKVVIHGDGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSE 540
           SL Q   L V  PP DKV I GDG  KAIFN E D+A++ IKAVDDP TLNKILY++P +
Sbjct: 481 SLAQPYLLEVATPPEDKVTILGDG--KAIFNVERDVATYIIKAVDDPRTLNKILYVRPPQ 530

Query: 541 NVLSINQLVALWENKIGIILHKTFVSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNR 600
           N  S   LVALWE K G IL K++VSE QLL +I EA++PLNL L+  YS+FVKGD TN 
Sbjct: 541 NTYSCVDLVALWEQKCGKILDKSYVSEQQLLNDIREASFPLNLRLSIFYSVFVKGDQTNF 530

Query: 601 EIEASVGLEATSLYPDVKYMTVDEYLNQFV 624
           +IE S G+EAT LYPDV+Y TVDE+LNQFV
Sbjct: 601 DIEPSFGVEATKLYPDVEYTTVDEFLNQFV 530

BLAST of Cp4.1LG15g05440 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 350.5 bits (898), Expect = 2.8e-96
Identity = 177/305 (58.03%), Postives = 218/305 (71.48%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++LV+G TGYIGKF+VEAS K GH TFALVR++ LSDP K   +Q FK +GV ++ GDL
Sbjct: 6   SKILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDL 65

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
            DHESLVKAIKQVDVVIST                       RF PSEFG DV+    V 
Sbjct: 66  NDHESLVKAIKQVDVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDRTSAVE 125

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQL--GVTAPPRDKVVIHGDG 523
           P KS F  K+QIRR +EAEGIPYTY ++ CF G+ +P+L Q   G+T+PPRDKV I GDG
Sbjct: 126 PAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVTILGDG 185

Query: 524 NPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTF 583
           N KA+ N EEDIA++TIKAVDDP TLNKILYIKPS N LS+N++V LWE KIG  L KT 
Sbjct: 186 NAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKSLEKTH 245

Query: 584 VSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDE 624
           + E QLLK+I E+  P+N++L+  +++FV GD TN  IE S G+EA+ LYPDVKY +VDE
Sbjct: 246 LPEEQLLKSIQESPIPINVVLSINHAVFVNGD-TNISIEPSFGVEASELYPDVKYTSVDE 305

BLAST of Cp4.1LG15g05440 vs. TAIR 10
Match: AT4G39230.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 334.0 bits (855), Expect = 2.7e-91
Identity = 169/304 (55.59%), Postives = 212/304 (69.74%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++L +G TGYIGK+IVEAS + GH T  LVR+S L+ P++S  I+ FK++GV  + GDL
Sbjct: 5   SKILFIGGTGYIGKYIVEASARSGHPTLVLVRNSTLTSPSRSSTIENFKNLGVQFLLGDL 64

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
            DH SLV +IKQ DVVIST                       RFFPSEFG DV+ V  V 
Sbjct: 65  DDHTSLVNSIKQADVVISTVGHSLLGHQYKIISAIKEAGNVKRFFPSEFGNDVDRVFTVE 124

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQLGVTAPPRDKVVIHGDGNP 523
           P KS + +K +IRR +EAEGIPYTYV    F G+ +P+L Q G T+ PRDKV++ GDGNP
Sbjct: 125 PAKSAYATKAKIRRTIEAEGIPYTYVSCNFFAGYFLPTLAQPGATSAPRDKVIVLGDGNP 184

Query: 524 KAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTFVS 583
           KA+FN EEDI ++TI AVDDP TLNKILYI+P  N  S N LV+LWENKIG  L + +V 
Sbjct: 185 KAVFNKEEDIGTYTINAVDDPRTLNKILYIRPPMNTYSFNDLVSLWENKIGKTLERIYVP 244

Query: 584 EHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDEYL 625
           E QLLK I E++ PLN+ML+  + +FVKG HT+ EIE S G+EA+ LYPDVKY TVDE L
Sbjct: 245 EEQLLKQIIESSPPLNVMLSLCHCVFVKGGHTSFEIEPSFGVEASELYPDVKYTTVDEIL 304

BLAST of Cp4.1LG15g05440 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 1.7e-90
Identity = 166/307 (54.07%), Postives = 213/307 (69.38%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++LV+G TG+IGK I+EASVK GH T ALVR+++LSDP K   +Q FK  GV ++ GDL
Sbjct: 6   SKILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDL 65

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
            DHESLVKAIKQ DVVIST                       RF PSEFG DV+    V 
Sbjct: 66  NDHESLVKAIKQADVVISTVGSMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDKSSAVE 125

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQL--GVTAPPRDKVVIHGDG 523
           P KS F  K+Q RR +EAEGIPYTY+++  F G+ +P+L QL  G+T+PPRDKV I GDG
Sbjct: 126 PAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVKIFGDG 185

Query: 524 NPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHKTF 583
           N KA+ N EEDIA++TIKAVDDP TLNK LYI P  N LS+N++V LWE KIG  + K +
Sbjct: 186 NVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKSVEKIY 245

Query: 584 VSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTVDE 626
           +SE Q+ K+I E+  P N++L+  +++FVKGD TN  IE S G EA+ LYPD+KY ++DE
Sbjct: 246 MSEEQIFKSIQESPVPFNVLLSINHAVFVKGDQTNFTIEPSFGFEASELYPDIKYTSIDE 305

BLAST of Cp4.1LG15g05440 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 312.4 bits (799), Expect = 8.3e-85
Identity = 166/319 (52.04%), Postives = 208/319 (65.20%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++LV+G TGY+G+FIVE S K G+ TFALVR+++LSDP KS  IQ FK +GV ++ GDL
Sbjct: 6   SKILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDL 65

Query: 404 FDHESLVKAIKQVDVVIST-----------------------RFFPSEFGYDVENVHGVG 463
            DHESLVKAIKQVDVVIST                       RF P+EFG DVE    V 
Sbjct: 66  NDHESLVKAIKQVDVVISTIGHKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVERTSAVE 125

Query: 464 PVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQL----------------GV 523
           P KS+F  KVQIRRA+EAEGIPYTYV+S C  GF + +L Q                   
Sbjct: 126 PAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAIIFGDK 185

Query: 524 TAPPRDKVVIHGDGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVA 583
             PPRDKV I GDGN K + N EED+A++ IKAVDD  TLNK LYI P  N+LS+N++V 
Sbjct: 186 NVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSMNEMVT 245

Query: 584 LWENKIGIILHKTFVSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEA 624
           LWE KIG  L KT +SE Q+LK+I     P+++  +  +++FVKGD T+  IE   G EA
Sbjct: 246 LWEKKIGKSLEKTHISEEQILKSIQ---VPIDVFKSINHAVFVKGDQTSFTIEPWFGEEA 305

BLAST of Cp4.1LG15g05440 vs. TAIR 10
Match: AT1G19540.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 307.4 bits (786), Expect = 2.7e-83
Identity = 159/308 (51.62%), Postives = 208/308 (67.53%), Query Frame = 0

Query: 344 NQVLVVGSTGYIGKFIVEASVKYGHRTFALVRDSALSDPAKSPIIQKFKSIGVIVVSGDL 403
           +++LV+G+TG IGK +VE S K GH TFALVR+++LSDP K+ ++++FK +GV ++ G L
Sbjct: 3   SKILVIGATGLIGKVLVEESAKSGHATFALVREASLSDPVKAQLVERFKDLGVTILYGSL 62

Query: 404 FDHESLVKAIKQVDVVIST-------------------------RFFPSEFGYDVENVHG 463
            D ESLVKAIKQVDVVIS                          RF PSEFG DV+    
Sbjct: 63  SDKESLVKAIKQVDVVISAVGRFQTEILNQTNIIDAIKESGNVKRFLPSEFGNDVDRTVA 122

Query: 464 VGPVKSIFESKVQIRRAVEAEGIPYTYVLSYCFNGFCVPSLGQ--LGVTAPPRDKVVIHG 523
           + P  S F +K QIRRA+EA  IPYTYV+S CF G  VP LGQ  L + +PPRDKV I+ 
Sbjct: 123 IEPTLSEFITKAQIRRAIEAAKIPYTYVVSGCFAGLFVPCLGQCHLRLRSPPRDKVSIYD 182

Query: 524 DGNPKAIFNNEEDIASFTIKAVDDPTTLNKILYIKPSENVLSINQLVALWENKIGIILHK 583
            GN KAI N EEDI ++T+KAVDDP TLNKILYI P   ++S N +V LWE KIG  L K
Sbjct: 183 TGNGKAIVNTEEDIVAYTLKAVDDPRTLNKILYIHPPNYIVSQNDMVGLWEEKIGKTLEK 242

Query: 584 TFVSEHQLLKNIHEAAYPLNLMLAFEYSIFVKGDHTNREIEASVGLEATSLYPDVKYMTV 625
           T+VSE +LLK I E+  P++ ++   ++I VK D T+  I+ S G+EA+ LYP+VKY +V
Sbjct: 243 TYVSEEELLKTIQESKPPMDFLVGLIHTILVKSDFTSFTIDPSFGVEASELYPEVKYTSV 302