Cp4.1LG15g01730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG15g01730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionisoflavone reductase homolog
LocationCp4.1LG15: 1447840 .. 1453394 (+)
RNA-Seq ExpressionCp4.1LG15g01730
SyntenyCp4.1LG15g01730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCGAAGCAAGAGAGAGTTTGAAGGCTATAAAGGGAGAAGGTGAGTTTGATTGGAAGAGGTAAAGGAAAGATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATTGTAAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGTCTCGACATTGAGAAGCTTCAGCTCCTGCTCTCCTTTAAGAGACAGGGGGCGGTTCTCGTGCCCGCCTCTTTCTCGGACTTTCACAGCCTTGTCGACGCAGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCAAGGTTTTCTTTCTTACCCTCTTCCACTTTCCTATTCATATTCATATTTAGGATTGAAATTTACGTGTAGGACACTGAGCAGTGTACCAACGAGGACACTGGCCCCCAAGGGAGTGGATTGTGAGATCCCATATCAATTAGAGAGGGGAATGAAGCATTCTTATAAGAGTGTGGAAACCTCTCTTTAGTAGACCTAGACACGTTTTAAAACCGTGAGGCTCATAGTGATACGTAATGGACCAAACCGGACAATATCTACTGGCGGCTGACTTAGGCTGTTACAAATGATATCAGAGTCAGACACTAGGCAAGATACCAGCGAGGATGCTGGGACCCCAAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGGAACAAATCATTCCTTATAAGGGTGTAGAAACCTCAACCTAGTAGATGACACGTTTTAAAATCATGAGGCTAACGATGATACGTAATGGACCAAAGCATACAATATCTGTTTGCAGTGGGCTTGAGCTGTTAGAGTTGACTTTGATCCTACTCTTAAAAAGATTAAAAATATTTAAAGTTTGTAATCGAAAAATATTTCTTACTGAACACTAGTCACGCTCTTTTTTTAATTCAAATTTAACAAACATATCAAGTATTAAACACATTTTTTACGATTGATAAACGAAATAGACACAAACTTTATACTTCATAAACTAAACTCTTAACCTACAATAAAATTTCTCGAGACTCAATTCGCTTTATCTTTTAGGTTGATCAGTGATTTAATATAAACGAACAGACTAATTTATCGAAATTCTTGATACAGAGATTCTTACCATCAGAGTACGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCCGGAAGAGAAACATTTGATCAAAAAATGATGATAAGAAAAGCAATAGAAGAAGCCAACATTCCTTTCACCTACGTCTCAGCCAATTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGCACACTCTTGTTCCTCCAACACATCAAGTTACCCTTTATGGAGATGGCAACGTTAAAGGTAACCCACCACCAATTATTCTTAATATAAACTCGAACGCTTCTCTTTTTATTTTCCAATGCTAGGTGTCGATGAGGTCGGTAGTCAAACTTTTAACGTTTTGGTCAATGATATATTGTTAAAGAATAATGTTGTTTAAATATAGAGATGTTCATTTAATTCACGAGTCCCATCGTATATGGAGTGAAAAATCTCCGTTTAAATAGAGAATGGGAAGGGAATAGAAAGAGATTTCTCTCTATTAGCTAAACGGGTATTTCACGTTGCCCTTCCCACCCTGCCCACCCCAAGCCCTGCAAATTCCAAGGGGAGGATCTCGATTTCGATCTCCGTGAAAATAAGTGAGAAATTTTATAAGAATGGGGATTGATCCCGTCCCCACTCTATTCTGTAGACATCTTAATTGAAATATAAATAATTTTATGACAAAATTTACAGCGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCACACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTATTAGAAAAGAACCGTGTTTCTGCTCAAGATTTTCTTACGAGGATGAAAGGTAAAGTTTTATATAATATATATAACACTCAAATTTTATATATAATGAAAATGTGATGTCCACCTCGGTTGGAAAGGAGAACAAAACACCCTTTGTAAGGGGTGTGGAAACCTTTCCCTAACATAGTTGTTTTAAAAATCTTGAGAGAAAGTCTAAAAATGACAATATCTGTTAGCGGTGGGCCTAAGCTGTTACAAATGGTATCAGAACCAGACACCAGGCGATTGCCAGTGAGGAGGCTGTTCCCCGAAGAGGGTAGACACGATGCGGTGTGCTAGTAAGGATGTTGGGCCCCAAAGGGGGGTGAATTATTATATCCCTCATCTGTTGGAGAGGAGAGCGAAACATCCTTTATAAGGGTGTGGAAACCTCTCCCAGTAAGGATGTTAGGCCCTGAAAGGGGGTGGATTGTGATATCCCACATCGGTTGGAGAGGAGAGTGAAACACCCTTTATAAGGGTGTGAAAACCTCCCGAAGGGGGGTGGATTGTGATGTCCCACATCGGTTGGGGAGGAGAGCGAAACACCATTTATAAGGGTGTGAAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGATTCAGGAGGTGGATTGTGATGTCCCACATCGGTTGGGGAGGAGAGCGAAACACCATTTATAAGGGTGTGAAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGATTCAGGAGGTGGATTGTGATGTCCCACATCGGTTGGGGAGGAGAGCGAAACACCATTTATAAGGGTGTGAAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGATTCAGGAGGTGGATTGTGATGTCCCACATCGGTTGGGGAGGAGAGCGAAACACCATTTATAAGGGTGTAAAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGAGAGAAAGTCTTAACGGTGGACCTGTATTCAAAAATAAAAAATAAAAAAGTATAATATTGATGCAGATCTGGACATAGCACAGCAGGCTGGAGTGGGACATTTCTATCACATATTCTTTGAAGGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAAGTGAACTACACTCGCATGGATGACTATCTCAAGATCTTCTTATGAGCACTTTAATGATTATCAACTTTATATGCAAAATGAAAATACTGTTAAATTTCTACTCTTTTTTCTTTTTAGTTTCCTTGTCGTGCCAAAAATATTTGGATTTGGATCCAATCCGTTACTTCATGACTTACTTCTCGATGAATGATATACTACCTACTATGTCAGTCGGTTGGTAGGCATGTTCAATAAATAATATGTGTTCCCAAAGAGCAATTCCTTTAGCTATGGCCTACTAGAGATTCCACATCGATTTGGGAGGAAAACGAAAAAAATTTTATAAGGGTATGAAAACTTCTTCCTAGCAAACGCGTTTAAGACCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAGAGGACAATATCTACTAGCGGTAGGCTTGAGCCGTTACATACGGTATCTAGTTGAAATTTCGAATTTGTTGAGTGCACTTATTAAGAAAAATATTTGGATTTGGATTCAATCCATTACTTCATGACATACTTCTCGATGGATGATATACTTACCTACTATACCGATCAGATGGTAGGCAAGTTCAATAAAGAACATGAGTTCCCAAGGAACAATTCCTTTAGCTAAGGCCTATTATTATAGTGTGAGATTCCACGTCGATTGGGGAGGAGAAGGAAAGGGTGTGAAAACCTCTTCCTAGCAGACGCGTTTTAAAATTCTTGAGAGAAAGCCGGAAAGGGAAAGTCCAAATATGACAATATCTGCTAGCGGAAGGTTTGGGCCGTTACATACAGTTTCTAGTCGAAATTTCGAATTCGTCGAGTGAGTTTAATAAGAAAAACTCGACCTTAAAACAAGGATCATTACCAAAACAAATATGTCGATATTTTACAACGAGACAAATGAGGGAAAAAACGTGAACATTATCATACCATACTTTAGTGATGGCCAAGAGTTGCTATGGTTACTTCAACCTTTTCAAATTCTTCATTCATATGAACAAAGGCAAGTGGCATATGCAGAAGAAGAGGTTATGAACTTACCATAGATTCTTCGAACGTTGCTCGACAAACTTCGAAATAGGTGGCGGAGAAAACGCTTCAAAAGCTGTATCCTGAAATGATCTACACTGGCATGGTTGATCACCTGAAGATGTTTTCATGAGGACAGCAAATAAAAGGTTGTGAAGTTATCTACCAAGTTAGCATAAACAGTATACATGCAATACAAAGCAAACCAACACAGATGTTAATTTCTTTACCCGTTTTGTTTAGTTTGATTCCCAGTATCTGCACTAAAACTTTACAATGTCAATTTATTTCCTTTTCGTTCTAACTTCGGTTTCGGTTTCCTTTCCATCGACTTTCTTAATGAGATATTTTTCTATTGGGAAAGATTAATATCATGTTTGGAATGATTTTTAATGACTTTTAAAAAAAAGTCGTCATTCCAAATAGGCCCTAATCCCTAAAGCATGTCTAGGATACTAGTATTTGATCCATCTCTTCGTGCTAATCAATTCTAAAGAAAGATTGATTAGATTATCCCCTACTTAAGGTTATAATGAATAATTAGCCGTCTTGATATGAAATGTCAAAATACCTAAATCATTTTTCTAAAATTAAATATACAATGTACTCATACAATTATTGGGTTTGACCCTATTGAACTAGTGATGATATTTTAACATGGACCCAGTCTAATACGCACAGAAGTAATTTCAACTAAGTTGCAACAACCGTAGTATCTTTATCATTTATCTCATTCTAAACTATTTCTTATAAAGTTTAAAACAGAATTGGAGAACTAACATGTTTGTGACAGAAAACAAAACTTGGAATTTCAGGACATGGGGCATGAATATATTGGATGAGATAAATGGAAGTTCTTGCTCCCTCCCAAAAATAATCTACGATTACATGTAATGATGCAAATGTCACTGAAGTATGTATTTTATGGCATGAGTATTACCTCGAACAAGGAAGCACTTTGGCCTCGAGTATTTCGGCCCCATTTGAACTGTAATCATAGCCACCTTTTGAATTCAAATCAAAGTTGATCATATTCCTTTCAACATTAAACTCGTTTGTGTCTTCTTCTCCAGCTTCCACTTTGCACATCTGGATGGGTGAGTCGCCCGTCTTCTTACAAGTATCGTTTACAGTCGTCATGCTTCCTCTCATTTCTTGTTTCTTAATTTTTTCCATTTGATCTGACAAAGCCACGTCAAAGCCTAATAGCTGCATTCCTGTGCTTTCAAGACTACCAAGGTCACTGTTAATGGCAAGTGCCAGACTCTTTTGTGCTTTCTCTAAGATATCTTGCAAGTATTTTCCTTGAGCTTCTATTCTCATTTGTAGTTTATTTTGTACCTGTCTTTACAATAGCCAACAAAGTAA

mRNA sequence

AAGCGAAGCAAGAGAGAGTTTGAAGGCTATAAAGGGAGAAGGTGAGTTTGATTGGAAGAGGTAAAGGAAAGATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATTGTAAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGTCTCGACATTGAGAAGCTTCAGCTCCTGCTCTCCTTTAAGAGACAGGGGGCGGTTCTCGTGCCCGCCTCTTTCTCGGACTTTCACAGCCTTGTCGACGCAGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCAAGAGATTCTTACCATCAGAGTACGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCCGGAAGAGAAACATTTGATCAAAAAATGATGATAAGAAAAGCAATAGAAGAAGCCAACATTCCTTTCACCTACGTCTCAGCCAATTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGCACACTCTTGTTCCTCCAACACATCAAGTTACCCTTTATGGAGATGGCAACGTTAAAGCGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCACACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTATTAGAAAAGAACCGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAACCAACAAAGTAA

Coding sequence (CDS)

ATGAAGGTTCTTGTGGTGGGTGGGACTGGTTATTTGGGGAAGAGGATTGTAAAGGCCAGCCTTTTGGAGGGGCACCAGACGTATGTGGTTCAACGGCCTGAGATTGGTCTCGACATTGAGAAGCTTCAGCTCCTGCTCTCCTTTAAGAGACAGGGGGCGGTTCTCGTGCCCGCCTCTTTCTCGGACTTTCACAGCCTTGTCGACGCAGTGAAGCGAGTCGACGTCGTGATATCGGCCATGTCCGGTGTCCATTTCAGGTCACATAACCTTCTCCTCCAACTCAAGCTTGTTGAAGCCATTCAAGTTGCTGGCAATGTCAAGAGATTCTTACCATCAGAGTACGGAATAGACCCAGCAAGAATGAGAGATGCACTCGAACCCGGAAGAGAAACATTTGATCAAAAAATGATGATAAGAAAAGCAATAGAAGAAGCCAACATTCCTTTCACCTACGTCTCAGCCAATTGCTTTGCCGGTTACTTTGCCGGTAGCCTGTCTCAAATGCACACTCTTGTTCCTCCAACACATCAAGTTACCCTTTATGGAGATGGCAACGTTAAAGCGGTATACGTGGACGAAGATGACATTGCAACGTACATCGTGAAGGCAGCAACAGATCCTCGAACTATGAACAAAACAGTGTATATTAGGCCTCCACACAACATTCTCTCCCAAAGAGAACTGATTCAGAAATGGGAAGCACTTTCTGGAAAAGTATTAGAAAAGAACCGTTGTTTGACAAATTTTGAAATAGGAGAAGGAGAAGAAGAAGCTTCAAAGCTTTATCCAGAACCAACAAAGTAA

Protein sequence

MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFSDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARMRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLYGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLEKNRCLTNFEIGEGEEEASKLYPEPTK
Homology
BLAST of Cp4.1LG15g01730 vs. ExPASy Swiss-Prot
Match: P52581 (Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 1.4e-110
Identity = 203/294 (69.05%), Postives = 231/294 (78.57%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           KVLVVGGTGY+G+RIVKASL  GH+T+++QRPEIGLDIEKLQ+LLSFK+QGA+LV ASFS
Sbjct: 5   KVLVVGGTGYVGRRIVKASLEHGHETFILQRPEIGLDIEKLQILLSFKKQGAILVEASFS 64

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLVDAVK VDVVI  MSGVHFRSHNLL QLKLVEAI+ AGN+KRFLPSE+G+DPA M
Sbjct: 65  DHKSLVDAVKLVDVVICTMSGVHFRSHNLLTQLKLVEAIKDAGNIKRFLPSEFGMDPALM 124

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLY 181
             ALEPGR TFD+KM +RKAIEEANIPFTY+SANCFAGYFAG+LSQM TL+PP  +V LY
Sbjct: 125 GHALEPGRVTFDEKMTVRKAIEEANIPFTYISANCFAGYFAGNLSQMKTLLPPRDKVLLY 184

Query: 182 GDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLE 241
           GDGNVK VY+DEDD+ATY +K   DPRT+NKTVY+RPP NIL+ +ELI+KWE L GK LE
Sbjct: 185 GDGNVKPVYMDEDDVATYTIKTIDDPRTLNKTVYLRPPENILTHKELIEKWEELIGKQLE 244

Query: 242 KNR-------------------------------CLTNFEIGEGEEEASKLYPE 265
           KN                                CLTNFEIGE  EEAS+LYPE
Sbjct: 245 KNSISEKDFLSTLKGLDFASQVGVGHFYHIFYEGCLTNFEIGENGEEASELYPE 298

BLAST of Cp4.1LG15g01730 vs. ExPASy Swiss-Prot
Match: A3R052 (Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PLR_Lp1 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 2.2e-103
Identity = 195/295 (66.10%), Postives = 223/295 (75.59%), Query Frame = 0

Query: 3   VLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFSD 62
           VLVVGGTGY+GKRIV ASL  GH TYV++RP  GLDIEKLQLLLSFK++GA LV ASFSD
Sbjct: 6   VLVVGGTGYIGKRIVSASLYLGHDTYVLKRPGTGLDIEKLQLLLSFKKRGAHLVEASFSD 65

Query: 63  FHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARMR 122
             SLV AV+ VDVVI  MSGVHFRSHN+LLQLKLVEAI+ AGNVKRF+PSE+G+DPARM 
Sbjct: 66  HDSLVRAVRLVDVVICTMSGVHFRSHNILLQLKLVEAIKEAGNVKRFIPSEFGMDPARMG 125

Query: 123 DALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLYG 182
            A+EPGRETFDQKM++RKAIEEANIP TY+SANCFAGYF G+LSQ+ TL PP+ +V +YG
Sbjct: 126 QAMEPGRETFDQKMVVRKAIEEANIPHTYISANCFAGYFVGNLSQLGTLTPPSDKVIIYG 185

Query: 183 DGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLEK 242
           DGNVK VYVDEDD+A Y +KA  D RT+NKTVY+RPP N++SQREL+  WE LSG  LEK
Sbjct: 186 DGNVKVVYVDEDDVAKYTIKAIEDDRTVNKTVYLRPPENMMSQRELVAVWEKLSGNQLEK 245

Query: 243 -------------------------------NRCLTNFEIG--EGEEEASKLYPE 265
                                            CLTNFEI    GEEEAS+LYPE
Sbjct: 246 IELPPQDFLALMEGTTVAEQAGIGHFYHIFYEGCLTNFEINAENGEEEASRLYPE 300

BLAST of Cp4.1LG15g01730 vs. ExPASy Swiss-Prot
Match: E6Y2X0 (Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=4006 GN=PLR_Lu2 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.2e-99
Identity = 186/294 (63.27%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           KVLV+GGTGYLGKR+V ASL  GH+TYV+QRPEIG+DIEK+QLLLSFK+ GA LV  SF+
Sbjct: 23  KVLVIGGTGYLGKRLVTASLAAGHETYVLQRPEIGVDIEKIQLLLSFKKAGASLVSGSFN 82

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D+ SLVDAVK VDVVI A+SGVH RSH +LLQLKLV+AI+ AGNVKRFLPSE+G DPA M
Sbjct: 83  DYRSLVDAVKLVDVVICAVSGVHIRSHQILLQLKLVDAIKEAGNVKRFLPSEFGTDPATM 142

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLY 181
            +A+EPGR TFD KM++RKAIEEA IPFTY+SANCFAGYF G L Q   ++P   QVTL 
Sbjct: 143 ENAMEPGRVTFDDKMVVRKAIEEAGIPFTYISANCFAGYFLGGLCQPGFILPSREQVTLL 202

Query: 182 GDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLE 241
           GDGN KAVYVDEDDIA Y +K   DPRT+NKTVYI+PP N+LSQRE++  WE   GK L+
Sbjct: 203 GDGNQKAVYVDEDDIARYTIKMIDDPRTLNKTVYIKPPKNVLSQREVVGIWEKYIGKELK 262

Query: 242 KNR-------------------------------CLTNFEIGEGEEEASKLYPE 265
           K                                 CLTNFEIG+   EA+KLYPE
Sbjct: 263 KTTLSVEEFLAMMKEQDYAEQVGLTHYYHVCYEGCLTNFEIGDEAGEATKLYPE 316

BLAST of Cp4.1LG15g01730 vs. ExPASy Swiss-Prot
Match: Q9FVQ6 (Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 3.0e-97
Identity = 184/294 (62.59%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVG TGY+GKRIV+A L EGH+TYV+QRPEIGL+IEK+QL LSFK+ GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNVKRFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLY 181
             AL PGRETFDQKM +R+AIE A IP+TYV   CFA YFAG+LSQM TL+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLE 241
           GDGNVK V+ DEDDIA Y  K   DPRT+NKTV IRPP N+L+Q EL+Q WE L+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KNR-------------------------------CLTNFEIGEGEEEASKLYPE 265
           K                                 CLT+ E+GE +EEAS LYP+
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGE-DEEASSLYPD 303

BLAST of Cp4.1LG15g01730 vs. ExPASy Swiss-Prot
Match: P93143 (Bifunctional pinoresinol-lariciresinol reductase OS=Forsythia intermedia OX=55183 GN=PLR_Fi1 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 2.6e-96
Identity = 179/295 (60.68%), Postives = 221/295 (74.92%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           KVL++GGTGYLG+R+VKASL +GH+TY++ RPEIG+DI+K+++L+SFK QGA LV  SF 
Sbjct: 5   KVLIIGGTGYLGRRLVKASLAQGHETYILHRPEIGVDIDKVEMLISFKMQGAHLVSGSFK 64

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           DF+SLV+AVK VDVVISA+SGVH RSH +LLQLKLVEAI+ AGNVKRFLPSE+G+DPA+ 
Sbjct: 65  DFNSLVEAVKLVDVVISAISGVHIRSHQILLQLKLVEAIKEAGNVKRFLPSEFGMDPAKF 124

Query: 122 RD-ALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 181
            D A+EPG+ T D+KM++RKAIE+A IPFTYVSANCFAGYF G L Q   ++P    V +
Sbjct: 125 MDTAMEPGKVTLDEKMVVRKAIEKAGIPFTYVSANCFAGYFLGGLCQFGKILPSRDFVII 184

Query: 182 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 241
           +GDGN KA+Y +EDDIATY +K   DPRT+NKT+YI PP NILSQRE++Q WE L GK L
Sbjct: 185 HGDGNKKAIYNNEDDIATYAIKTINDPRTLNKTIYISPPKNILSQREVVQTWEKLIGKEL 244

Query: 242 EK-------------------------------NRCLTNFEIGEGEEEASKLYPE 265
           +K                                 CLT+FEIG+ EEEASKLYPE
Sbjct: 245 QKITLSKEDFLASVKELEYAQQVGLSHYHDVNYQGCLTSFEIGD-EEEASKLYPE 298

BLAST of Cp4.1LG15g01730 vs. NCBI nr
Match: XP_023511514.1 (isoflavone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 506 bits (1304), Expect = 8.64e-180
Identity = 264/295 (89.49%), Postives = 264/295 (89.49%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKNR                               CLTNFEIGEGEEEASKLYPE
Sbjct: 241 EKNRVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPE 295

BLAST of Cp4.1LG15g01730 vs. NCBI nr
Match: XP_022963989.1 (isoflavone reductase homolog [Cucurbita moschata])

HSP 1 Score: 491 bits (1263), Expect = 1.52e-173
Identity = 257/295 (87.12%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKM++RKAIEEANIPFTYVSANCFAGYFAGSLSQMHTL PPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLAPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRP  NILSQRELIQKWEALS KVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPRDNILSQRELIQKWEALSEKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKN                                CLTNFEIGEGEEEASKLYPE
Sbjct: 241 EKNSVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPE 295

BLAST of Cp4.1LG15g01730 vs. NCBI nr
Match: XP_022967298.1 (isoflavone reductase homolog [Cucurbita maxima])

HSP 1 Score: 490 bits (1262), Expect = 2.16e-173
Identity = 257/295 (87.12%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKM IRKAIEEANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRPP NILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKNR                               CLTNFEIGEGEEEAS+LYPE
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPE 295

BLAST of Cp4.1LG15g01730 vs. NCBI nr
Match: KAG6571626.1 (hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 484 bits (1247), Expect = 4.17e-171
Identity = 254/295 (86.10%), Postives = 257/295 (87.12%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNV+RFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVERFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKM IRKAIE+ANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQV L
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEDANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVAL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRPP NILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKN                                CLTNFEIGEGEEEASKLYPE
Sbjct: 241 EKNSVSAQDFLARMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPE 295

BLAST of Cp4.1LG15g01730 vs. NCBI nr
Match: XP_022963983.1 (isoflavone reductase homolog [Cucurbita moschata])

HSP 1 Score: 480 bits (1235), Expect = 2.71e-169
Identity = 247/295 (83.73%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGG+GYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN+KRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           M+DALEPGRETFDQKM +RKAIEEANIPFTYVSANCFAGYFAGSLS+MHTLVPPTHQVTL
Sbjct: 121 MKDALEPGRETFDQKMTVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATY+VKAATDPRTMNKTVYIRPPHNILSQRE+++KWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EK                                 C+TNFE+ E +EEASKLYPE
Sbjct: 241 EKKSISAQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVRE-DEEASKLYPE 294

BLAST of Cp4.1LG15g01730 vs. ExPASy TrEMBL
Match: A0A6J1HLT4 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 7.38e-174
Identity = 257/295 (87.12%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKM++RKAIEEANIPFTYVSANCFAGYFAGSLSQMHTL PPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLAPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRP  NILSQRELIQKWEALS KVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPRDNILSQRELIQKWEALSEKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKN                                CLTNFEIGEGEEEASKLYPE
Sbjct: 241 EKNSVSAQDFLTRMKDLDIAQQAGVGHFYHIFFEGCLTNFEIGEGEEEASKLYPE 295

BLAST of Cp4.1LG15g01730 vs. ExPASy TrEMBL
Match: A0A6J1HUP1 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 1.05e-173
Identity = 257/295 (87.12%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           MRDALEPGRETFDQKM IRKAIEEANIPFTYVSANCFAGYFAGSLSQM +LVPPTHQVTL
Sbjct: 121 MRDALEPGRETFDQKMTIRKAIEEANIPFTYVSANCFAGYFAGSLSQMQSLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVK VYVDEDDIATYIVKAATDPRTMNKTVYIRPP NILSQRELIQKWEALSGKVL
Sbjct: 181 YGDGNVKVVYVDEDDIATYIVKAATDPRTMNKTVYIRPPDNILSQRELIQKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EKNR                               CLTNFEIGEGEEEAS+LYPE
Sbjct: 241 EKNRVSAQDFLARMKDLDIAQQAGVGHFYHLFFEGCLTNFEIGEGEEEASRLYPE 295

BLAST of Cp4.1LG15g01730 vs. ExPASy TrEMBL
Match: A0A6J1HJH3 (isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 SV=1)

HSP 1 Score: 480 bits (1235), Expect = 1.31e-169
Identity = 247/295 (83.73%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGG+GYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN+KRFLPSEYGIDPAR
Sbjct: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           M+DALEPGRETFDQKM +RKAIEEANIPFTYVSANCFAGYFAGSLS+MHTLVPPTHQVTL
Sbjct: 121 MKDALEPGRETFDQKMTVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATY+VKAATDPRTMNKTVYIRPPHNILSQRE+++KWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EK                                 C+TNFE+ E +EEASKLYPE
Sbjct: 241 EKKSISAQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVRE-DEEASKLYPE 294

BLAST of Cp4.1LG15g01730 vs. ExPASy TrEMBL
Match: A0A6J1HWD5 (isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV=1)

HSP 1 Score: 478 bits (1229), Expect = 1.08e-168
Identity = 246/295 (83.39%), Postives = 259/295 (87.80%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           MKVLVVGG+GYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF
Sbjct: 1   MKVLVVGGSGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SDF SLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGN+KRFLPSEYGIDPAR
Sbjct: 61  SDFQSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNIKRFLPSEYGIDPAR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           M+DALEPGRETFDQKM++RKAIEEANIPFTYVSANCFAGYFAGSLS+MHTLVPPTHQVTL
Sbjct: 121 MKDALEPGRETFDQKMIVRKAIEEANIPFTYVSANCFAGYFAGSLSEMHTLVPPTHQVTL 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVKAVYVDEDDIATY+VKAATDPRTMNKTVYIRPPHNILSQRE+++KWEALSGKVL
Sbjct: 181 YGDGNVKAVYVDEDDIATYVVKAATDPRTMNKTVYIRPPHNILSQREMVEKWEALSGKVL 240

Query: 241 EKNR-------------------------------CLTNFEIGEGEEEASKLYPE 264
           EK                                 C+TNFE+ E +EEASKLYPE
Sbjct: 241 EKKSVSPQDFLARMKDLDLGQRAGLGHFYHIFFDGCMTNFEVRE-DEEASKLYPE 294

BLAST of Cp4.1LG15g01730 vs. ExPASy TrEMBL
Match: A0A5A7VJ56 (Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold392G00250 PE=4 SV=1)

HSP 1 Score: 446 bits (1147), Expect = 3.43e-156
Identity = 229/295 (77.63%), Postives = 247/295 (83.73%), Query Frame = 0

Query: 1   MKVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASF 60
           M+VLVVGGTGYLGKRIVKASLLEGH+TYVVQRPEIGLDIEKLQLLLSFKRQGA+LVPASF
Sbjct: 1   MRVLVVGGTGYLGKRIVKASLLEGHETYVVQRPEIGLDIEKLQLLLSFKRQGAILVPASF 60

Query: 61  SDFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPAR 120
           SD  SLVDAVKRVDVVISA+SGVHFRSH++LLQLKLVEAIQ AGN+KRFLPSEYGIDP+R
Sbjct: 61  SDIQSLVDAVKRVDVVISALSGVHFRSHSILLQLKLVEAIQAAGNIKRFLPSEYGIDPSR 120

Query: 121 MRDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTL 180
           M +A+E GR+TFDQKM+IRKAIEEANIPFTYVSANCFAGYFA +LSQM TLVPPTHQVT+
Sbjct: 121 MGNAIEQGRDTFDQKMVIRKAIEEANIPFTYVSANCFAGYFAANLSQMETLVPPTHQVTV 180

Query: 181 YGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVL 240
           YGDGNVK +Y DEDDIA Y+VKAATDPRT+NKTVYIRPP NILSQRELIQ WE LSGKVL
Sbjct: 181 YGDGNVKVIYTDEDDIARYVVKAATDPRTLNKTVYIRPPQNILSQRELIQIWEKLSGKVL 240

Query: 241 EK-------------------------------NRCLTNFEIGEGEEEASKLYPE 264
           EK                                 CLTNFEIGE  EEASKL+PE
Sbjct: 241 EKISVSAQDFLANIKDLDEAQQAGAGHFYEIFFKGCLTNFEIGEAAEEASKLFPE 295

BLAST of Cp4.1LG15g01730 vs. TAIR 10
Match: AT1G32100.1 (pinoresinol reductase 1 )

HSP 1 Score: 356.3 bits (913), Expect = 2.1e-98
Identity = 184/294 (62.59%), Postives = 216/294 (73.47%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVG TGY+GKRIV+A L EGH+TYV+QRPEIGL+IEK+QL LSFK+ GA +V  SFS
Sbjct: 11  RVLVVGATGYIGKRIVRACLAEGHETYVLQRPEIGLEIEKVQLFLSFKKLGARIVEGSFS 70

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV AVK VDVV+SAMSGVHFRSHN+L+QLKLVEAI+ AGNVKRFLPSE+G+DP RM
Sbjct: 71  DHQSLVSAVKLVDVVVSAMSGVHFRSHNILVQLKLVEAIKEAGNVKRFLPSEFGMDPPRM 130

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLY 181
             AL PGRETFDQKM +R+AIE A IP+TYV   CFA YFAG+LSQM TL+PP  +V +Y
Sbjct: 131 GHALPPGRETFDQKMEVRQAIEAAGIPYTYVVGACFAAYFAGNLSQMVTLLPPKEKVNIY 190

Query: 182 GDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLE 241
           GDGNVK V+ DEDDIA Y  K   DPRT+NKTV IRPP N+L+Q EL+Q WE L+GK LE
Sbjct: 191 GDGNVKVVFADEDDIAKYTAKTLNDPRTLNKTVNIRPPDNVLTQLELVQIWEKLTGKELE 250

Query: 242 KNR-------------------------------CLTNFEIGEGEEEASKLYPE 265
           K                                 CLT+ E+GE +EEAS LYP+
Sbjct: 251 KTNIAAQDFLANIEQMEIPHQAGIGHFYHIFYEGCLTDHEVGE-DEEASSLYPD 303

BLAST of Cp4.1LG15g01730 vs. TAIR 10
Match: AT4G13660.1 (pinoresinol reductase 2 )

HSP 1 Score: 338.2 bits (866), Expect = 6.0e-93
Identity = 177/294 (60.20%), Postives = 212/294 (72.11%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           +VLVVGGTG LG+RIV A L EGH+TYV+QRPEIG+DIEK+QLLLSFKR GA LV  SFS
Sbjct: 11  RVLVVGGTGSLGRRIVSACLAEGHETYVLQRPEIGVDIEKVQLLLSFKRLGAHLVEGSFS 70

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV AVK+VDVV+SAMSGVHFR+HN+ +QLKLV AI+ AGNVKRFLPSE+G+DP+RM
Sbjct: 71  DHQSLVSAVKQVDVVVSAMSGVHFRTHNIPVQLKLVAAIKEAGNVKRFLPSEFGMDPSRM 130

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMHTLVPPTHQVTLY 181
             A+ PG ETFDQKM IR AI+ A I  TY+   CFA YF G+LSQM TL PP ++V +Y
Sbjct: 131 GHAMPPGSETFDQKMEIRNAIKAAGISHTYLVGACFAAYFGGNLSQMGTLFPPKNKVDIY 190

Query: 182 GDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKVLE 241
           GDGNVK V+VDEDD+A Y  K   DPRT+NKTVY+RP  NIL+Q EL+Q WE L+ K LE
Sbjct: 191 GDGNVKVVFVDEDDMAKYTAKTLNDPRTLNKTVYVRPTDNILTQMELVQIWEKLTEKELE 250

Query: 242 K-------------------------------NRCLTNFEIGEGEEEASKLYPE 265
           K                                 CLT+ E+G+ +EEA+KLYP+
Sbjct: 251 KTYVSGNDFLADIEDKEISHQAGLGHFYHIYYEGCLTDHEVGD-DEEATKLYPD 303

BLAST of Cp4.1LG15g01730 vs. TAIR 10
Match: AT1G75280.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 208.4 bits (529), Expect = 7.2e-54
Identity = 112/243 (46.09%), Postives = 150/243 (61.73%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTGY+GK +V+AS   GH T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYIGKFLVEASAKAGHSTFALVREATLSDPVKGKTVQSFKDLGVTILHGDLN 66

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV A+K+VDVVIS +      S  +L Q K++ AI+ AGNVKRFLPSE+G+D  R 
Sbjct: 67  DHESLVKAIKQVDVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGVDVDR- 126

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMH--TLVPPTHQVT 181
             A+EP +  F  K+ IR+ IE   IP+TY    CF GY+  +L Q       PP  +VT
Sbjct: 127 TSAVEPAKSAFAGKIQIRRTIEAEGIPYTYAVTGCFGGYYLPTLVQFEPGLTSPPRDKVT 186

Query: 182 LYGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKV 241
           + GDGN KAV   E+DIA Y +KA  DPRT+NK +YI+P +N LS  E++  WE   GK 
Sbjct: 187 ILGDGNAKAVINKEEDIAAYTIKAVDDPRTLNKILYIKPSNNTLSMNEIVTLWEKKIGKS 243

Query: 242 LEK 243
           LEK
Sbjct: 247 LEK 243

BLAST of Cp4.1LG15g01730 vs. TAIR 10
Match: AT1G75290.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.1e-53
Identity = 110/243 (45.27%), Postives = 154/243 (63.37%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTG++GK I++AS+  GH T  + R     D  K + + +FK  G  L+    +
Sbjct: 7   KILVIGGTGHIGKLIIEASVKAGHSTLALVREASLSDPNKGKTVQNFKDFGVTLLHGDLN 66

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV A+K+ DVVIS +      S  +L Q K++ AI+ AGNVKRFLPSE+G+D  + 
Sbjct: 67  DHESLVKAIKQADVVISTVG-----SMQILDQTKIISAIKEAGNVKRFLPSEFGMDVDK- 126

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQMH--TLVPPTHQVT 181
             A+EP +  F +K+  R+ IE   IP+TY+  N FAGY+  +L Q+      PP  +V 
Sbjct: 127 SSAVEPAKSAFGRKLQTRRDIEAEGIPYTYLVTNYFAGYYLPTLVQLEPGLTSPPRDKVK 186

Query: 182 LYGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQRELIQKWEALSGKV 241
           ++GDGNVKAV   E+DIA Y +KA  DPRT+NKT+YI PP+N LS  E++  WE   GK 
Sbjct: 187 IFGDGNVKAVINKEEDIAAYTIKAVDDPRTLNKTLYINPPNNTLSMNEIVTLWEKKIGKS 243

Query: 242 LEK 243
           +EK
Sbjct: 247 VEK 243

BLAST of Cp4.1LG15g01730 vs. TAIR 10
Match: AT1G75300.1 (NmrA-like negative transcriptional regulator family protein )

HSP 1 Score: 201.4 bits (511), Expect = 8.8e-52
Identity = 113/257 (43.97%), Postives = 155/257 (60.31%), Query Frame = 0

Query: 2   KVLVVGGTGYLGKRIVKASLLEGHQTYVVQRPEIGLDIEKLQLLLSFKRQGAVLVPASFS 61
           K+LV+GGTGY+G+ IV+ S   G+ T+ + R     D  K + + SFK  G  ++    +
Sbjct: 7   KILVIGGTGYMGEFIVEGSAKAGNPTFALVREASLSDPVKSKTIQSFKDLGVTILHGDLN 66

Query: 62  DFHSLVDAVKRVDVVISAMSGVHFRSHNLLLQLKLVEAIQVAGNVKRFLPSEYGIDPARM 121
           D  SLV A+K+VDVVIS +         +  Q K++ AI+ AGNVKRFLP+E+GID  R 
Sbjct: 67  DHESLVKAIKQVDVVISTIG-----HKQIFDQTKIISAIKEAGNVKRFLPAEFGIDVER- 126

Query: 122 RDALEPGRETFDQKMMIRKAIEEANIPFTYVSANCFAGYFAGSLSQM------HTL---- 181
             A+EP +  F  K+ IR+AIE   IP+TYV +NC AG++  +L Q       HT     
Sbjct: 127 TSAVEPAKSLFAGKVQIRRAIEAEGIPYTYVVSNCSAGFYLRTLLQFESGLISHTRDKAI 186

Query: 182 ------VPPTHQVTLYGDGNVKAVYVDEDDIATYIVKAATDPRTMNKTVYIRPPHNILSQ 241
                 VPP  +VT+ GDGN K V   E+D+A Y++KA  D RT+NKT+YI PP+NILS 
Sbjct: 187 IFGDKNVPPRDKVTILGDGNAKVVINKEEDVAAYMIKAVDDLRTLNKTLYISPPNNILSM 246

Query: 242 RELIQKWEALSGKVLEK 243
            E++  WE   GK LEK
Sbjct: 247 NEMVTLWEKKIGKSLEK 257

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P525811.4e-11069.05Isoflavone reductase homolog OS=Lupinus albus OX=3870 PE=2 SV=1[more]
A3R0522.2e-10366.10Bifunctional pinoresinol-lariciresinol reductase OS=Linum perenne OX=35941 GN=PL... [more]
E6Y2X03.2e-9963.27Bifunctional pinoresinol-lariciresinol reductase 2 OS=Linum usitatissimum OX=400... [more]
Q9FVQ63.0e-9762.59Pinoresinol reductase 1 OS=Arabidopsis thaliana OX=3702 GN=PRR1 PE=1 SV=1[more]
P931432.6e-9660.68Bifunctional pinoresinol-lariciresinol reductase OS=Forsythia intermedia OX=5518... [more]
Match NameE-valueIdentityDescription
XP_023511514.18.64e-18089.49isoflavone reductase homolog [Cucurbita pepo subsp. pepo][more]
XP_022963989.11.52e-17387.12isoflavone reductase homolog [Cucurbita moschata][more]
XP_022967298.12.16e-17387.12isoflavone reductase homolog [Cucurbita maxima][more]
KAG6571626.14.17e-17186.10hypothetical protein SDJN03_28354, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022963983.12.71e-16983.73isoflavone reductase homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1HLT47.38e-17487.12isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464122 PE=4 ... [more]
A0A6J1HUP11.05e-17387.12isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466861 PE=4 SV... [more]
A0A6J1HJH31.31e-16983.73isoflavone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111464118 PE=4 ... [more]
A0A6J1HWD51.08e-16883.39isoflavone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111466862 PE=4 SV... [more]
A0A5A7VJ563.43e-15677.63Isoflavone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
AT1G32100.12.1e-9862.59pinoresinol reductase 1 [more]
AT4G13660.16.0e-9360.20pinoresinol reductase 2 [more]
AT1G75280.17.2e-5446.09NmrA-like negative transcriptional regulator family protein [more]
AT1G75290.12.1e-5345.27NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G75300.18.8e-5243.97NmrA-like negative transcriptional regulator family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 1..180
e-value: 5.2E-48
score: 165.3
NoneNo IPR availableGENE3D3.90.25.10coord: 190..248
e-value: 1.3E-13
score: 53.1
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 244..264
NoneNo IPR availablePANTHERPTHR43349:SF47BIFUNCTIONAL PINORESINOL-LARICIRESINOL REDUCTASE-LIKEcoord: 2..243
NoneNo IPR availablePANTHERPTHR43349PINORESINOL REDUCTASE-RELATEDcoord: 244..264
coord: 2..243
NoneNo IPR availableCDDcd05259PCBER_SDR_acoord: 2..244
e-value: 2.48E-63
score: 198.296
IPR008030NmrA-like domainPFAMPF05368NmrAcoord: 3..238
e-value: 1.7E-57
score: 194.6
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 2..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG15g01730.1Cp4.1LG15g01730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009807 lignan biosynthetic process
molecular_function GO:0016491 oxidoreductase activity