Cp4.1LG15g01020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG15g01020 vs. ExPASy Swiss-Prot
Match: O64495 (Subtilisin-like protease SBT1.2 OS=Arabidopsis thaliana OX=3702 GN=SBT1.2 PE=2 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 1.4e-301
Identity = 511/767 (66.62%), Postives = 626/767 (81.62%), Query Frame = 0

Query: 13  FLCFLLLQVQANASNL---QTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL----SVEED 72
           FLC + L   +++S +   QTY++QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 73  CSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGV---C 132
            SSRLLYSY +A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS+KFLG+    
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 133 TQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRK 192
             GV  KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SSSCNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 193 LIGAKFFIKGHHVASSPPS--DTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 252
           LIGA+FFI+GH VA+SP    +  +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 253 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAI 312
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 313 GSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGE 372
           G+FRA++ GISVICAAGNNGP +SSVAN APW++TIGAGTLDR FPA+VRL+NGK + GE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 373 SMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           S+YPG     A +E+E++Y+TGG  GSE CL+GSLPRE++ GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 492
           VKE+GG AMILANTEIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 493 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFS 552
           VIGR+RAP VAQFS+RGPS  NPS LKPD+IAPGVNIIAAWPQNL PTGLP DSRR NF+
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 553 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 612
           VMSGTSMSCPHVSGI ALI SA+P W+PAAIKSA+MTTAD+ DR GK I DGNKPA VFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 613 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 672
           +GAGHVNP +AI+PGLVY+I+P DY+ +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 673 LNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNES 732
           LNYPS+AV+FK G T++M++R++TNVGSPNS+Y V V APEG+ V V P+RLVFKHV+++
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 733 LSYKVWFMSEKRREGRKVSS-TEGHLTWLHSENSKYRVRSPIVVTWK 767
           LSY+VWF+ +K+  G KV+S  +G LTW++S N   RVRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Cp4.1LG15g01020 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.2e-186
Identity = 364/764 (47.64%), Postives = 485/764 (63.48%), Query Frame = 0

Query: 11  TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRL 70
           TI   FL L +   A   +TY+I+++      S F +   W+ S L        +  S L
Sbjct: 12  TIITTFLFLLLHTTAK--KTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSL 71

Query: 71  LYSYSNAMEGFAAQLSETELE-YLKKLPDVVAVRADRKYEIQTTYSHKFLGVCTQ-GVCY 130
           LY+Y+ +  GF+A L  TE +  L     ++ +  D  Y + TT + +FLG+ ++ GV  
Sbjct: 72  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 131

Query: 131 KSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKF 190
             S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ 
Sbjct: 132 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 191

Query: 191 FIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPG 250
           F KG  +AS     + +E VSPRD  GHGTHTS+TAAG++V  AS  G  AG A+GMA  
Sbjct: 192 FSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 251

Query: 251 AHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFRAVQH 310
           A +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D IAIG+F A++ 
Sbjct: 252 ARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMER 311

Query: 311 GISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYPGNKF 370
           G+ V C+AGN+GPT++SVANVAPW+ T+GAGTLDR FPA   L NGK + G S+Y G   
Sbjct: 312 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--V 371

Query: 371 NQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAA 430
              TK LELVY  G    S LCL GSL    V GK+VVCDRGVN R EKG +V+++GG  
Sbjct: 372 GMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 431

Query: 431 MILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRTRAP 490
           MI+ANT  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +P
Sbjct: 432 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 491

Query: 491 SVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFSVMSGTSMS 550
            VA FSSRGP+   P  LKPDVI PGVNI+A W   + PTGL +DSRR+ F++MSGTSMS
Sbjct: 492 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 551

Query: 551 CPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDG--NKPASVFAMGAGHV 610
           CPH+SG+A L+ +AHP+W+P+AIKSA+MTTA V D    P+ D   N  ++ +A G+GHV
Sbjct: 552 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 611

Query: 611 NPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPS 670
           +P +A+ PGLVYDI   +Y+  LC L YT   I  I+   +V+C K  + +    LNYPS
Sbjct: 612 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPS 671

Query: 671 MAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNESLSYKV 730
            +V+F  G      +R++TNVG+ +SVY+V V     V + VKP +L FK V E   Y V
Sbjct: 672 FSVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTV 731

Query: 731 WFMSEKRREGRKVSSTE----GHLTWLHSENSKYRVRSPIVVTW 766
            F+S+K      VS T     G +TW    N ++ VRSP+  +W
Sbjct: 732 TFVSKK-----GVSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Cp4.1LG15g01020 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 7.4e-181
Identity = 352/761 (46.25%), Postives = 486/761 (63.86%), Query Frame = 0

Query: 12  IFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLL 71
           + LC     V +++S+  TY++ +    + SS F+    W+ S L    S+ +  S+ LL
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLR---SISD--SAELL 72

Query: 72  YSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGV--CTQGVCYK 131
           Y+Y NA+ GF+ +L++ E + L   P V++V  + +YE+ TT +  FLG+   T  +  +
Sbjct: 73  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPE 132

Query: 132 SSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFF 191
           +      +VGVLDTGVWPES S+SD    PIP  W+GGC+ G +F +S CNRKLIGA+FF
Sbjct: 133 AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFF 192

Query: 192 IKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGA 251
            +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V GAS+ G  +G A+GMAP A
Sbjct: 193 ARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 252

Query: 252 HIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFRAVQHG 311
            +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +AIG+F A++ G
Sbjct: 253 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 312

Query: 312 ISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYPGNKFN 371
           I V C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NGK   G S++ G    
Sbjct: 313 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL- 372

Query: 372 QATKELELVYL--TGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGA 431
              K L  +Y           LC+ G+L  E+V+GK+V+CDRG+N R +KG +VK +GG 
Sbjct: 373 -PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 432

Query: 432 AMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRTRA 491
            MILANT  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   GTV+G   +
Sbjct: 433 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 492

Query: 492 PSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFSVMSGTSM 551
           P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW     PTGL  DSRR  F+++SGTSM
Sbjct: 493 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 552

Query: 552 SCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNKPASVFAMGAGH 611
           SCPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T + GKP+LD    KP++ F  GAGH
Sbjct: 553 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 612

Query: 612 VNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYPS 671
           V+PT A +PGL+YD+   DY+  LC L YT  +I  ++  N +C      +    LNYPS
Sbjct: 613 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVA-DLNYPS 672

Query: 672 MAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTA-PEGVSVRVKPQRLVFKHVNESLSYK 731
            AV    G  +   +R +T+VG   + Y V+VT+   GV + V+P  L FK  NE  SY 
Sbjct: 673 FAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 732

Query: 732 VWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTW 766
           V F  +  +     S++ G + W    + K+ V SP+ ++W
Sbjct: 733 VTFTVDSSKPSG--SNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Cp4.1LG15g01020 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 2.8e-180
Identity = 346/776 (44.59%), Postives = 489/776 (63.02%), Query Frame = 0

Query: 13  FLCFLLLQV-----QANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCS 72
           F  F LL +      A++SN  TY++ +  +    S+F +   W+ S L    S      
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEA-KPSIFPTHFHWYTSSLASLTS----SP 64

Query: 73  SRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGVCT---Q 132
             ++++Y     GF+A+L+  +   L   P V++V  ++   + TT S +FLG+ +    
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 133 GVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLI 192
           G+  +S  G   ++GV+DTGVWPE PSF D  + P+P KW+G C   QDF  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 193 GAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQG 252
           GA+FF  G+   +   ++T  E+ SPRDS GHGTHT+S +AG  V  AS  G   GVA G
Sbjct: 185 GARFFCGGYEATNGKMNETT-EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAG 244

Query: 253 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFR 312
           MAP A +A YKVCW SGCY SDI+AA D+A+ DGVD++SLS+GG  +P++ D IAIG+F 
Sbjct: 245 MAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFG 304

Query: 313 AVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYP 372
           A+  GI V  +AGN GP   +V NVAPW+TT+GAGT+DR FPA V+L NGK I G S+Y 
Sbjct: 305 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 364

Query: 373 GNKFNQATKELELVY----LTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           G   +   +   LVY    L G    S LCL+GSL    V+GK+V+CDRG+N R+ KG+I
Sbjct: 365 GPGLDPG-RMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 424

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTTNPKAR 492
           V+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ +P A 
Sbjct: 425 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 484

Query: 493 IQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDS 552
           I F GT +G   AP VA FS+RGP+P  P  LKPDVIAPG+NI+AAWP  + P+G+  D+
Sbjct: 485 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 544

Query: 553 RRSNFSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGN- 612
           RR+ F+++SGTSM+CPHVSG+AAL+ +AHP W+PAAI+SA++TTA   D  G+P++D + 
Sbjct: 545 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 604

Query: 613 -KPASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKV 672
              +SV   G+GHV+PT+A+DPGLVYDI  YDY+  LC   YT + I  IT     C   
Sbjct: 605 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 664

Query: 673 LQMNKGFTLNYPSMAVVFKHGTTSKMVS---RQLTNVGSPNSVYEVEVTAPEGVSVRVKP 732
            +      LNYPS +VVF+    SKM +   R +TNVG  +SVYE+++  P G +V V+P
Sbjct: 665 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 724

Query: 733 QRLVFKHVNESLSYKVWFMSEKRREGRKVSSTE-GHLTWLHSENSKYRVRSPIVVT 765
           ++L F+ V + LS+ V   + + +     ++ E GH+ W    + K  V SP+VVT
Sbjct: 725 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVVT 770

BLAST of Cp4.1LG15g01020 vs. ExPASy Swiss-Prot
Match: Q84WS0 (Subtilisin-like protease SBT1.1 OS=Arabidopsis thaliana OX=3702 GN=SBTI1.1 PE=1 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 2.7e-175
Identity = 354/781 (45.33%), Postives = 476/781 (60.95%), Query Frame = 0

Query: 2   DFNSQMGFPTIFLCFLLLQVQAN-ASNLQTYMIQ---LHPQGLTSSVFESKLQWHLSFLE 61
           D    M F +  + F L+   +N +S  QTY+I       + + +S+F S          
Sbjct: 14  DNRPMMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTSTKHIVTSLFNSL--------- 73

Query: 62  QTLSVEEDCSS--RLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYS 121
           QT ++ +D  S   + Y Y NAM GF+A L++ +L+ +K     ++   D    + TTYS
Sbjct: 74  QTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYS 133

Query: 122 HKFLGV-CTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDF 181
           H+FLG+    G+  ++S+    I+G++DTG+ PE  SF D+ M P+P +WRG C EG +F
Sbjct: 134 HEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNF 193

Query: 182 NSSSCNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASV 241
           +SS CN+K+IGA  F KG+       ++T  ++ S RD+ GHGTHT+STAAG  V  A+ 
Sbjct: 194 SSSECNKKIIGASAFYKGYESIVGKINETT-DFRSTRDAQGHGTHTASTAAGDIVPKANY 253

Query: 242 FGNGAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFF 301
           FG   G+A GM   + IA YK CW  GC S+D++AA+D AI DGVD++SLSLGG   PF+
Sbjct: 254 FGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFY 313

Query: 302 DDNIAIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG 361
            D IAI  F A+Q  I V C+AGN+GPT S+V+N APW+ T+ A   DRTFPAIVR+ N 
Sbjct: 314 VDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNR 373

Query: 362 KAIPGESMYPGNKFNQATKELELVY--LTGGQMGSELCLKGSLPREQVEGKMVVCDRGVN 421
           K++ G S+Y G    ++ K L L +    G + G+  C++ SL RE VEGK+V+C RG +
Sbjct: 374 KSLVGSSLYKG----KSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS 433

Query: 422 GRSEKGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPK 481
           GR+ KG+ VK SGGAAM+L +TE   EE L D HVLPA  +GF++   L  Y+    N  
Sbjct: 434 GRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANAT 493

Query: 482 ARIQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPE 541
           A ++F GT  G T AP VA FSSRGPS   P   KPD+ APG+NI+A W    +P+ L  
Sbjct: 494 ASVRFRGTAYGAT-APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRS 553

Query: 542 DSRRSNFSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD- 601
           D RR  F+++SGTSM+CPH+SGIAALI S H  W+PA IKSAIMTTA +TD   +PI D 
Sbjct: 554 DPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDR 613

Query: 602 ----GNKPASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNV 661
                   A+ FA GAG+V+PTRA+DPGLVYD    DY+ +LC L YT   I + +  N 
Sbjct: 614 GAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNY 673

Query: 662 SCHKVLQMNKGFTLNYPSMAVVFKHGTTSKMV--SRQLTNVGSPNSVYEVEVTAPEGVSV 721
           +C     +     LNYPS AV   +G   K V   R +TNVGSP   Y V V  P+GV V
Sbjct: 674 TCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKV 733

Query: 722 RVKPQRLVFKHVNESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTW 767
           RV+P+ L F+   E LSY V + +E  R     SS+ G L W+     KY VRSPI VTW
Sbjct: 734 RVEPKVLKFQKARERLSYTVTYDAEASRNSS--SSSFGVLVWI---CDKYNVRSPIAVTW 774

BLAST of Cp4.1LG15g01020 vs. NCBI nr
Match: XP_023553603.1 (subtilisin-like protease SBT1.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1538 bits (3981), Expect = 0.0
Identity = 767/767 (100.00%), Postives = 767/767 (100.00%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCN 180
           VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCN
Sbjct: 121 VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCN 180

Query: 181 RKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 240
           RKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG
Sbjct: 181 RKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 240

Query: 241 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAI 300
           VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAI
Sbjct: 241 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAI 300

Query: 301 GSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGE 360
           GSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGE
Sbjct: 301 GSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGE 360

Query: 361 SMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 420
           SMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI
Sbjct: 361 SMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 420

Query: 421 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 480
           VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT
Sbjct: 421 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 480

Query: 481 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFS 540
           VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFS
Sbjct: 481 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFS 540

Query: 541 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 600
           VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA
Sbjct: 541 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 600

Query: 601 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 660
           MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT
Sbjct: 601 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 660

Query: 661 LNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNES 720
           LNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNES
Sbjct: 661 LNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNES 720

Query: 721 LSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           LSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 LSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767

BLAST of Cp4.1LG15g01020 vs. NCBI nr
Match: XP_022963543.1 (subtilisin-like protease SBT1.2 [Cucurbita moschata])

HSP 1 Score: 1506 bits (3898), Expect = 0.0
Identity = 754/769 (98.05%), Postives = 762/769 (99.09%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDF SQM F TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFYSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSF DSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+AIP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSM+VVFKHGTTSKMVSR+LTNVGSPNSVYEVEVTAPEGVSVRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of Cp4.1LG15g01020 vs. NCBI nr
Match: XP_022967188.1 (subtilisin-like protease SBT1.2 [Cucurbita maxima])

HSP 1 Score: 1494 bits (3869), Expect = 0.0
Identity = 748/769 (97.27%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQM F TIFLCFL LQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMVFSTIFLCFLWLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASSPPSD VQEYVSPRDSHGHGTHTSSTAAGA VAGASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGALVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
            GVAQGMAPG HIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 VGVAQGMAPGTHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+ IP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEPIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSM+CPHVSGIAALI SAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMACPHVSGIAALIQSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKP+DYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPFDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSMAVVFKHGTTSKMVSR+LTNVGSPNSVYEVEVTAPEGVSVRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           +SLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 QSLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of Cp4.1LG15g01020 vs. NCBI nr
Match: KAG6571549.1 (Subtilisin-like protease 1.2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1489 bits (3854), Expect = 0.0
Identity = 747/763 (97.90%), Postives = 754/763 (98.82%), Query Frame = 0

Query: 7   MGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDC 66
           M F TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDC
Sbjct: 1   MVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDC 60

Query: 67  SSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG--VCTQ 126
           SSRLLYSYSNAMEGFAAQLSETELEYLKKL DVVAVRADRKYEIQTTYSHKFLG  VCTQ
Sbjct: 61  SSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLGLSVCTQ 120

Query: 127 GVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLI 186
           GVCYKSSMGQGAIVGVLDTGVWPESPSF DSKMPPIPQKWRGGCQEGQDFNSSSCNRKLI
Sbjct: 121 GVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLI 180

Query: 187 GAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQG 246
           GAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQG
Sbjct: 181 GAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQG 240

Query: 247 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFR 306
           MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+IAIGSFR
Sbjct: 241 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSIAIGSFR 300

Query: 307 AVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYP 366
           A+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSN +AIPGESMYP
Sbjct: 301 AMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNREAIPGESMYP 360

Query: 367 GNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKES 426
           GNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKES
Sbjct: 361 GNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKES 420

Query: 427 GGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGR 486
           GGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFGGTVIGR
Sbjct: 421 GGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFGGTVIGR 480

Query: 487 TRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFSVMSG 546
           TRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSNF+VMSG
Sbjct: 481 TRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSNFTVMSG 540

Query: 547 TSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFAMGAG 606
           TSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFAMGAG
Sbjct: 541 TSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFAMGAG 600

Query: 607 HVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYP 666
           HVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYP
Sbjct: 601 HVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYP 660

Query: 667 SMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNESLSYK 726
           SM+VVFKHGTTSKMVSR LTNVGSPNSVYEVEVTAPEGVSVRVKP+RLVFKHVNESLSYK
Sbjct: 661 SMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVNESLSYK 720

Query: 727 VWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           VWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 VWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 763

BLAST of Cp4.1LG15g01020 vs. NCBI nr
Match: KAG7011294.1 (Subtilisin-like protease SBT1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1410 bits (3650), Expect = 0.0
Identity = 709/726 (97.66%), Postives = 718/726 (98.90%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQM F TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKL DVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLADVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASSPPSD VQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+AIP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGG+MGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGKMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSM+VVFKHGTTSKMVSR LTNVGSPNSVYEVEVTAPEGVSV+VKP+RLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRWLTNVGSPNSVYEVEVTAPEGVSVQVKPRRLVFKHVN 720

Query: 721 ESLSYK 724
           ESLSYK
Sbjct: 721 ESLSYK 726

BLAST of Cp4.1LG15g01020 vs. ExPASy TrEMBL
Match: A0A6J1HI32 (subtilisin-like protease SBT1.2 OS=Cucurbita moschata OX=3662 GN=LOC111463837 PE=3 SV=1)

HSP 1 Score: 1506 bits (3898), Expect = 0.0
Identity = 754/769 (98.05%), Postives = 762/769 (99.09%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDF SQM F TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFYSQMVFSTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSF DSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFGDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+AIP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEAIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTT+PKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTDPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSM+VVFKHGTTSKMVSR+LTNVGSPNSVYEVEVTAPEGVSVRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMSVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of Cp4.1LG15g01020 vs. ExPASy TrEMBL
Match: A0A6J1HUC6 (subtilisin-like protease SBT1.2 OS=Cucurbita maxima OX=3661 GN=LOC111466803 PE=3 SV=1)

HSP 1 Score: 1494 bits (3869), Expect = 0.0
Identity = 748/769 (97.27%), Postives = 757/769 (98.44%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFNSQM F TIFLCFL LQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL
Sbjct: 1   MDFNSQMVFSTIFLCFLWLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG
Sbjct: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS
Sbjct: 121 LSVCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASSPPSD VQEYVSPRDSHGHGTHTSSTAAGA VAGASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSPPSDIVQEYVSPRDSHGHGTHTSSTAAGALVAGASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
            GVAQGMAPG HIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 VGVAQGMAPGTHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG+ IP
Sbjct: 301 AIGSFRAMQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGEPIP 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGL EDSRRSN
Sbjct: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLLEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSM+CPHVSGIAALI SAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV
Sbjct: 541 FTVMSGTSMACPHVSGIAALIQSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPTRAIDPGLVYDIKP+DYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTRAIDPGLVYDIKPFDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSMAVVFKHGTTSKMVSR+LTNVGSPNSVYEVEVTAPEGVSVRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMAVVFKHGTTSKMVSRRLTNVGSPNSVYEVEVTAPEGVSVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           +SLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN
Sbjct: 721 QSLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 769

BLAST of Cp4.1LG15g01020 vs. ExPASy TrEMBL
Match: A0A5A7T534 (Subtilisin-like protease SBT1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold154G00500 PE=3 SV=1)

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 691/769 (89.86%), Postives = 727/769 (94.54%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++MGF  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             V TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISV+CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNG+AI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT LPEDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSM+V+FKHGTTSKMVSR+LTNVGS NS+YEV+VTAPEGV VRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           +SL+YKVWFMSEK +EGRKV  TEG LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 QSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Cp4.1LG15g01020 vs. ExPASy TrEMBL
Match: A0A1S3BHA2 (subtilisin-like protease SBT1.2 OS=Cucumis melo OX=3656 GN=LOC103489836 PE=3 SV=1)

HSP 1 Score: 1384 bits (3581), Expect = 0.0
Identity = 691/769 (89.86%), Postives = 727/769 (94.54%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++MGF  +FL FL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQTL
Sbjct: 1   MDFNTRMGF-LLFLYFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQTL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQL+ETELEYLKKLPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLTETELEYLKKLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             V TQGV  KSSMGQGAIVG+LDTGVWPESPSFSDSKMPPIPQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGVWQKSSMGQGAIVGLLDTGVWPESPSFSDSKMPPIPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD VQEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVVQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAI DGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAISDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISV+CAAGNNGPTQSSVANVAPWITTIGAGTLDR FPAIVRLSNG+AI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPTQSSVANVAPWITTIGAGTLDRRFPAIVRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PT LPEDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTSLPEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHKVLQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKVLQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPSM+V+FKHGTTSKMVSR+LTNVGS NS+YEV+VTAPEGV VRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSMSVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           +SL+YKVWFMSEK +EGRKV  TEG LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 QSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Cp4.1LG15g01020 vs. ExPASy TrEMBL
Match: A0A0A0LDY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G610820 PE=3 SV=1)

HSP 1 Score: 1382 bits (3578), Expect = 0.0
Identity = 686/769 (89.21%), Postives = 728/769 (94.67%), Query Frame = 0

Query: 1   MDFNSQMGFPTIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL 60
           MDFN++MGF  +FLCFL L VQ N S LQTY+IQLHP GL +SVF+SKLQWHLSFLEQ+L
Sbjct: 1   MDFNARMGF-LLFLCFLSLLVQPNTSTLQTYIIQLHPHGLITSVFDSKLQWHLSFLEQSL 60

Query: 61  SVEEDCSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLG 120
           S EED SSRLLYSYSNAMEGFAAQLSETELEYLK+LPDVVAVR DRKY+IQTTYSHKFLG
Sbjct: 61  SAEEDSSSRLLYSYSNAMEGFAAQLSETELEYLKRLPDVVAVREDRKYQIQTTYSHKFLG 120

Query: 121 --VCTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSS 180
             V TQG+  KSSMGQGAIVGVLDTGVWPESPSFSDSKMPP+PQKWRG CQEGQDFNSS+
Sbjct: 121 LSVGTQGLRQKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPVPQKWRGACQEGQDFNSSN 180

Query: 181 CNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNG 240
           CNRKLIGAKFFIKGHHVASS PSD  QEYVSPRDSHGHGTHTSSTAAGASVA ASVFGNG
Sbjct: 181 CNRKLIGAKFFIKGHHVASSLPSDVAQEYVSPRDSHGHGTHTSSTAAGASVADASVFGNG 240

Query: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNI 300
           AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDD+I
Sbjct: 241 AGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDSI 300

Query: 301 AIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIP 360
           AIGSFRA+QHGISV+CAAGNNGP QSSVANVAPWITTIGAGTLDR FPAI+RLSNG+AI 
Sbjct: 301 AIGSFRAMQHGISVVCAAGNNGPIQSSVANVAPWITTIGAGTLDRRFPAIIRLSNGEAIY 360

Query: 361 GESMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKG 420
           GESMYPGNKF QATKELE+VYLTGGQMG ELCLKGSLPRE+V+GKMVVCDRGVNGRSEKG
Sbjct: 361 GESMYPGNKFKQATKELEVVYLTGGQMGGELCLKGSLPREKVQGKMVVCDRGVNGRSEKG 420

Query: 421 QIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFG 480
           QIVKESGGAAMILAN+EINLEEDLVDVHVLPATLIGFAEANRLKAYINTT+NPKARIQFG
Sbjct: 421 QIVKESGGAAMILANSEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTSNPKARIQFG 480

Query: 481 GTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSN 540
           GTVIGR+RAPSVAQFSSRGPS +NPSTLKPDVIAPGVNIIAAWPQNL PTGLPEDSRRSN
Sbjct: 481 GTVIGRSRAPSVAQFSSRGPSLSNPSTLKPDVIAPGVNIIAAWPQNLGPTGLPEDSRRSN 540

Query: 541 FSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASV 600
           F+VMSGTSM+CPHVSGI ALIHSAHPKWTPAAIKSAIMTTADVTD FGK ILDGNKPA V
Sbjct: 541 FTVMSGTSMACPHVSGITALIHSAHPKWTPAAIKSAIMTTADVTDHFGKQILDGNKPADV 600

Query: 601 FAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKG 660
           FAMGAGHVNPT+AIDPGLVYDIKPY+Y+IHLC LGYTHSEIFIITHMNVSCHK+LQMNKG
Sbjct: 601 FAMGAGHVNPTKAIDPGLVYDIKPYEYIIHLCALGYTHSEIFIITHMNVSCHKILQMNKG 660

Query: 661 FTLNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVN 720
           FTLNYPS++V+FKHGTTSKMVSR+LTNVGS NS+YEV+VTAPEGV VRVKP+RLVFKHVN
Sbjct: 661 FTLNYPSISVIFKHGTTSKMVSRRLTNVGSTNSIYEVKVTAPEGVRVRVKPRRLVFKHVN 720

Query: 721 ESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTWKN 767
           +SL+YKVWFMSEK +EGRKV  TEG LTW+H ENSKY+VRSPIVVTWKN
Sbjct: 721 QSLNYKVWFMSEKGKEGRKVRFTEGDLTWIHCENSKYKVRSPIVVTWKN 768

BLAST of Cp4.1LG15g01020 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 1036.6 bits (2679), Expect = 1.0e-302
Identity = 511/767 (66.62%), Postives = 626/767 (81.62%), Query Frame = 0

Query: 13  FLCFLLLQVQANASNL---QTYMIQLHPQGLTSSVFESKLQWHLSFLEQTL----SVEED 72
           FLC + L   +++S +   QTY++QLHP   T+  F SK  WHLSFL++ +      EE+
Sbjct: 7   FLCIIFLLFCSSSSEILQKQTYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEE 66

Query: 73  CSSRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGV---C 132
            SSRLLYSY +A+EGFAAQL+E+E E L+  P+VVAVR D   ++QTTYS+KFLG+    
Sbjct: 67  PSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFG 126

Query: 133 TQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRK 192
             GV  KS  GQG I+GVLDTGVWPESPSF D+ MP IP+KW+G CQEG+ F+SSSCNRK
Sbjct: 127 NSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRK 186

Query: 193 LIGAKFFIKGHHVASSPPS--DTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAG 252
           LIGA+FFI+GH VA+SP    +  +EY+S RDS GHGTHT+ST  G+SV+ A+V GNGAG
Sbjct: 187 LIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAG 246

Query: 253 VAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAI 312
           VA+GMAPGAHIAVYKVCWF+GCYSSDI+AA+D AI+D VD+LSLSLGGFP+P +DD IAI
Sbjct: 247 VARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAI 306

Query: 313 GSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGE 372
           G+FRA++ GISVICAAGNNGP +SSVAN APW++TIGAGTLDR FPA+VRL+NGK + GE
Sbjct: 307 GTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGE 366

Query: 373 SMYPGNKFNQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           S+YPG     A +E+E++Y+TGG  GSE CL+GSLPRE++ GKMV+CDRGVNGRSEKG+ 
Sbjct: 367 SLYPGKGIKNAGREVEVIYVTGGDKGSEFCLRGSLPREEIRGKMVICDRGVNGRSEKGEA 426

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGT 492
           VKE+GG AMILANTEIN EED +DVH+LPATLIG+ E+  LKAY+N T  PKARI FGGT
Sbjct: 427 VKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGT 486

Query: 493 VIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFS 552
           VIGR+RAP VAQFS+RGPS  NPS LKPD+IAPGVNIIAAWPQNL PTGLP DSRR NF+
Sbjct: 487 VIGRSRAPEVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSRRVNFT 546

Query: 553 VMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGNKPASVFA 612
           VMSGTSMSCPHVSGI ALI SA+P W+PAAIKSA+MTTAD+ DR GK I DGNKPA VFA
Sbjct: 547 VMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGNKPAGVFA 606

Query: 613 MGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFT 672
           +GAGHVNP +AI+PGLVY+I+P DY+ +LC LG+T S+I  ITH NVSC+ +L+ N GF+
Sbjct: 607 IGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFS 666

Query: 673 LNYPSMAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNES 732
           LNYPS+AV+FK G T++M++R++TNVGSPNS+Y V V APEG+ V V P+RLVFKHV+++
Sbjct: 667 LNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVKAPEGIKVIVNPKRLVFKHVDQT 726

Query: 733 LSYKVWFMSEKRREGRKVSS-TEGHLTWLHSENSKYRVRSPIVVTWK 767
           LSY+VWF+ +K+  G KV+S  +G LTW++S N   RVRSPI VT K
Sbjct: 727 LSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRVRSPISVTLK 773

BLAST of Cp4.1LG15g01020 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 654.8 bits (1688), Expect = 8.4e-188
Identity = 364/764 (47.64%), Postives = 485/764 (63.48%), Query Frame = 0

Query: 11  TIFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRL 70
           TI   FL L +   A   +TY+I+++      S F +   W+ S L        +  S L
Sbjct: 12  TIITTFLFLLLHTTAK--KTYIIRVNHSDKPES-FLTHHDWYTSQL--------NSESSL 71

Query: 71  LYSYSNAMEGFAAQLSETELE-YLKKLPDVVAVRADRKYEIQTTYSHKFLGVCTQ-GVCY 130
           LY+Y+ +  GF+A L  TE +  L     ++ +  D  Y + TT + +FLG+ ++ GV  
Sbjct: 72  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 131

Query: 131 KSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKF 190
             S   G I+GVLDTGVWPES SF D+ MP IP KW+G C+ G DF+S  CN+KLIGA+ 
Sbjct: 132 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 191

Query: 191 FIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPG 250
           F KG  +AS     + +E VSPRD  GHGTHTS+TAAG++V  AS  G  AG A+GMA  
Sbjct: 192 FSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 251

Query: 251 AHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFRAVQH 310
           A +A YKVCW +GC+ SDI+AAMD AI DGVD+LSLSLGG   P++ D IAIG+F A++ 
Sbjct: 252 ARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMER 311

Query: 311 GISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYPGNKF 370
           G+ V C+AGN+GPT++SVANVAPW+ T+GAGTLDR FPA   L NGK + G S+Y G   
Sbjct: 312 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSG--V 371

Query: 371 NQATKELELVYLTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGAA 430
              TK LELVY  G    S LCL GSL    V GK+VVCDRGVN R EKG +V+++GG  
Sbjct: 372 GMGTKPLELVYNKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 431

Query: 431 MILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRTRAP 490
           MI+ANT  + EE + D H+LPA  +G    + L+ Y+ + + P A + F GTV+    +P
Sbjct: 432 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 491

Query: 491 SVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFSVMSGTSMS 550
            VA FSSRGP+   P  LKPDVI PGVNI+A W   + PTGL +DSRR+ F++MSGTSMS
Sbjct: 492 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 551

Query: 551 CPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDG--NKPASVFAMGAGHV 610
           CPH+SG+A L+ +AHP+W+P+AIKSA+MTTA V D    P+ D   N  ++ +A G+GHV
Sbjct: 552 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 611

Query: 611 NPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIF-IITHMNVSCHKVLQMNKGFTLNYPS 670
           +P +A+ PGLVYDI   +Y+  LC L YT   I  I+   +V+C K  + +    LNYPS
Sbjct: 612 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK--KFSDPGQLNYPS 671

Query: 671 MAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTAPEGVSVRVKPQRLVFKHVNESLSYKV 730
            +V+F  G      +R++TNVG+ +SVY+V V     V + VKP +L FK V E   Y V
Sbjct: 672 FSVLF-GGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTV 731

Query: 731 WFMSEKRREGRKVSSTE----GHLTWLHSENSKYRVRSPIVVTW 766
            F+S+K      VS T     G +TW    N ++ VRSP+  +W
Sbjct: 732 TFVSKK-----GVSMTNKAEFGSITW---SNPQHEVRSPVAFSW 751

BLAST of Cp4.1LG15g01020 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 635.6 bits (1638), Expect = 5.3e-182
Identity = 352/761 (46.25%), Postives = 486/761 (63.86%), Query Frame = 0

Query: 12  IFLCFLLLQVQANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCSSRLL 71
           + LC     V +++S+  TY++ +    + SS F+    W+ S L    S+ +  S+ LL
Sbjct: 13  LLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSS-FDLHSNWYDSSLR---SISD--SAELL 72

Query: 72  YSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGV--CTQGVCYK 131
           Y+Y NA+ GF+ +L++ E + L   P V++V  + +YE+ TT +  FLG+   T  +  +
Sbjct: 73  YTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPE 132

Query: 132 SSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLIGAKFF 191
           +      +VGVLDTGVWPES S+SD    PIP  W+GGC+ G +F +S CNRKLIGA+FF
Sbjct: 133 AGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFF 192

Query: 192 IKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQGMAPGA 251
            +G+  ++  P D  +E  SPRD  GHGTHTSSTAAG+ V GAS+ G  +G A+GMAP A
Sbjct: 193 ARGYE-STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRA 252

Query: 252 HIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFRAVQHG 311
            +AVYKVCW  GC+SSDI+AA+D AI D V++LS+SLGG    ++ D +AIG+F A++ G
Sbjct: 253 RVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERG 312

Query: 312 ISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYPGNKFN 371
           I V C+AGN GP+ SS++NVAPWITT+GAGTLDR FPA+  L NGK   G S++ G    
Sbjct: 313 ILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEAL- 372

Query: 372 QATKELELVYL--TGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQIVKESGGA 431
              K L  +Y           LC+ G+L  E+V+GK+V+CDRG+N R +KG +VK +GG 
Sbjct: 373 -PDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGV 432

Query: 432 AMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPKARIQFGGTVIGRTRA 491
            MILANT  N EE + D H+LPAT +G    + ++ Y+ T  NP A I   GTV+G   +
Sbjct: 433 GMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPS 492

Query: 492 PSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDSRRSNFSVMSGTSM 551
           P VA FSSRGP+   P+ LKPD+IAPGVNI+AAW     PTGL  DSRR  F+++SGTSM
Sbjct: 493 PVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSM 552

Query: 552 SCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD--GNKPASVFAMGAGH 611
           SCPHVSG+AAL+ S HP+W+PAAI+SA+MTTA  T + GKP+LD    KP++ F  GAGH
Sbjct: 553 SCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGH 612

Query: 612 VNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKVLQMNKGFTLNYPS 671
           V+PT A +PGL+YD+   DY+  LC L YT  +I  ++  N +C      +    LNYPS
Sbjct: 613 VSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVA-DLNYPS 672

Query: 672 MAVVFKHGTTSKMVSRQLTNVGSPNSVYEVEVTA-PEGVSVRVKPQRLVFKHVNESLSYK 731
            AV    G  +   +R +T+VG   + Y V+VT+   GV + V+P  L FK  NE  SY 
Sbjct: 673 FAVNV-DGVGAYKYTRTVTSVGGAGT-YSVKVTSETTGVKISVEPAVLNFKEANEKKSYT 732

Query: 732 VWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTW 766
           V F  +  +     S++ G + W    + K+ V SP+ ++W
Sbjct: 733 VTFTVDSSKPSG--SNSFGSIEW---SDGKHVVGSPVAISW 756

BLAST of Cp4.1LG15g01020 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 633.6 bits (1633), Expect = 2.0e-181
Identity = 346/776 (44.59%), Postives = 489/776 (63.02%), Query Frame = 0

Query: 13  FLCFLLLQV-----QANASNLQTYMIQLHPQGLTSSVFESKLQWHLSFLEQTLSVEEDCS 72
           F  F LL +      A++SN  TY++ +  +    S+F +   W+ S L    S      
Sbjct: 5   FYFFFLLTLSSPSSSASSSNSLTYIVHVDHEA-KPSIFPTHFHWYTSSLASLTS----SP 64

Query: 73  SRLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYSHKFLGVCT---Q 132
             ++++Y     GF+A+L+  +   L   P V++V  ++   + TT S +FLG+ +    
Sbjct: 65  PSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKA 124

Query: 133 GVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDFNSSSCNRKLI 192
           G+  +S  G   ++GV+DTGVWPE PSF D  + P+P KW+G C   QDF  S+CNRKL+
Sbjct: 125 GLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLV 184

Query: 193 GAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASVFGNGAGVAQG 252
           GA+FF  G+   +   ++T  E+ SPRDS GHGTHT+S +AG  V  AS  G   GVA G
Sbjct: 185 GARFFCGGYEATNGKMNETT-EFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAG 244

Query: 253 MAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFFDDNIAIGSFR 312
           MAP A +A YKVCW SGCY SDI+AA D+A+ DGVD++SLS+GG  +P++ D IAIG+F 
Sbjct: 245 MAPKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFG 304

Query: 313 AVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNGKAIPGESMYP 372
           A+  GI V  +AGN GP   +V NVAPW+TT+GAGT+DR FPA V+L NGK I G S+Y 
Sbjct: 305 AIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYG 364

Query: 373 GNKFNQATKELELVY----LTGGQMGSELCLKGSLPREQVEGKMVVCDRGVNGRSEKGQI 432
           G   +   +   LVY    L G    S LCL+GSL    V+GK+V+CDRG+N R+ KG+I
Sbjct: 365 GPGLDPG-RMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEI 424

Query: 433 VKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYIN------TTTNPKAR 492
           V+++GG  MI+AN   + E  + D HVLPAT +G +  + ++ YI+      ++ +P A 
Sbjct: 425 VRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTAT 484

Query: 493 IQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPEDS 552
           I F GT +G   AP VA FS+RGP+P  P  LKPDVIAPG+NI+AAWP  + P+G+  D+
Sbjct: 485 IVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDN 544

Query: 553 RRSNFSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILDGN- 612
           RR+ F+++SGTSM+CPHVSG+AAL+ +AHP W+PAAI+SA++TTA   D  G+P++D + 
Sbjct: 545 RRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDEST 604

Query: 613 -KPASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNVSCHKV 672
              +SV   G+GHV+PT+A+DPGLVYDI  YDY+  LC   YT + I  IT     C   
Sbjct: 605 GNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGA 664

Query: 673 LQMNKGFTLNYPSMAVVFKHGTTSKMVS---RQLTNVGSPNSVYEVEVTAPEGVSVRVKP 732
            +      LNYPS +VVF+    SKM +   R +TNVG  +SVYE+++  P G +V V+P
Sbjct: 665 RRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEP 724

Query: 733 QRLVFKHVNESLSYKVWFMSEKRREGRKVSSTE-GHLTWLHSENSKYRVRSPIVVT 765
           ++L F+ V + LS+ V   + + +     ++ E GH+ W    + K  V SP+VVT
Sbjct: 725 EKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVW---SDGKRNVTSPLVVT 770

BLAST of Cp4.1LG15g01020 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 617.1 bits (1590), Expect = 1.9e-176
Identity = 354/781 (45.33%), Postives = 476/781 (60.95%), Query Frame = 0

Query: 2   DFNSQMGFPTIFLCFLLLQVQAN-ASNLQTYMIQ---LHPQGLTSSVFESKLQWHLSFLE 61
           D    M F +  + F L+   +N +S  QTY+I       + + +S+F S          
Sbjct: 14  DNRPMMFFRSFIVFFFLIFFASNVSSRKQTYVIHTVTTSTKHIVTSLFNSL--------- 73

Query: 62  QTLSVEEDCSS--RLLYSYSNAMEGFAAQLSETELEYLKKLPDVVAVRADRKYEIQTTYS 121
           QT ++ +D  S   + Y Y NAM GF+A L++ +L+ +K     ++   D    + TTYS
Sbjct: 74  QTENINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYS 133

Query: 122 HKFLGV-CTQGVCYKSSMGQGAIVGVLDTGVWPESPSFSDSKMPPIPQKWRGGCQEGQDF 181
           H+FLG+    G+  ++S+    I+G++DTG+ PE  SF D+ M P+P +WRG C EG +F
Sbjct: 134 HEFLGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNF 193

Query: 182 NSSSCNRKLIGAKFFIKGHHVASSPPSDTVQEYVSPRDSHGHGTHTSSTAAGASVAGASV 241
           +SS CN+K+IGA  F KG+       ++T  ++ S RD+ GHGTHT+STAAG  V  A+ 
Sbjct: 194 SSSECNKKIIGASAFYKGYESIVGKINETT-DFRSTRDAQGHGTHTASTAAGDIVPKANY 253

Query: 242 FGNGAGVAQGMAPGAHIAVYKVCWFSGCYSSDIVAAMDSAIRDGVDILSLSLGGFPLPFF 301
           FG   G+A GM   + IA YK CW  GC S+D++AA+D AI DGVD++SLSLGG   PF+
Sbjct: 254 FGQAKGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFY 313

Query: 302 DDNIAIGSFRAVQHGISVICAAGNNGPTQSSVANVAPWITTIGAGTLDRTFPAIVRLSNG 361
            D IAI  F A+Q  I V C+AGN+GPT S+V+N APW+ T+ A   DRTFPAIVR+ N 
Sbjct: 314 VDPIAIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNR 373

Query: 362 KAIPGESMYPGNKFNQATKELELVY--LTGGQMGSELCLKGSLPREQVEGKMVVCDRGVN 421
           K++ G S+Y G    ++ K L L +    G + G+  C++ SL RE VEGK+V+C RG +
Sbjct: 374 KSLVGSSLYKG----KSLKNLPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS 433

Query: 422 GRSEKGQIVKESGGAAMILANTEINLEEDLVDVHVLPATLIGFAEANRLKAYINTTTNPK 481
           GR+ KG+ VK SGGAAM+L +TE   EE L D HVLPA  +GF++   L  Y+    N  
Sbjct: 434 GRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANAT 493

Query: 482 ARIQFGGTVIGRTRAPSVAQFSSRGPSPTNPSTLKPDVIAPGVNIIAAWPQNLAPTGLPE 541
           A ++F GT  G T AP VA FSSRGPS   P   KPD+ APG+NI+A W    +P+ L  
Sbjct: 494 ASVRFRGTAYGAT-APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRS 553

Query: 542 DSRRSNFSVMSGTSMSCPHVSGIAALIHSAHPKWTPAAIKSAIMTTADVTDRFGKPILD- 601
           D RR  F+++SGTSM+CPH+SGIAALI S H  W+PA IKSAIMTTA +TD   +PI D 
Sbjct: 554 DPRRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDR 613

Query: 602 ----GNKPASVFAMGAGHVNPTRAIDPGLVYDIKPYDYVIHLCVLGYTHSEIFIITHMNV 661
                   A+ FA GAG+V+PTRA+DPGLVYD    DY+ +LC L YT   I + +  N 
Sbjct: 614 GAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNY 673

Query: 662 SCHKVLQMNKGFTLNYPSMAVVFKHGTTSKMV--SRQLTNVGSPNSVYEVEVTAPEGVSV 721
           +C     +     LNYPS AV   +G   K V   R +TNVGSP   Y V V  P+GV V
Sbjct: 674 TCASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKV 733

Query: 722 RVKPQRLVFKHVNESLSYKVWFMSEKRREGRKVSSTEGHLTWLHSENSKYRVRSPIVVTW 767
           RV+P+ L F+   E LSY V + +E  R     SS+ G L W+     KY VRSPI VTW
Sbjct: 734 RVEPKVLKFQKARERLSYTVTYDAEASRNSS--SSSFGVLVWI---CDKYNVRSPIAVTW 774